Rh3CG225300

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0001177
Physical Location & Seq
Reverse (-)
21128091 .. 21129563
1473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG225300.1

Sequence Viewer

Length: 684 bp
ATGTCAGAGAAACAAGCCCACTTGAATGGAGCCAACTACGGCCCATCCGTCCCGCCACGAGAGACCTACCACCGCCCCGGCCGCGGTGGAGGTCTCCTCAGCTGCTGCTGCAGCTGCATCTTCAGTCTCGTCTTCAAGCTCATCTTCGCCGCAGTTGTCTTCTTGGGCTTAGCCTTCCTCGTCTTCTGGCTCATAGTCAGGCCAACCGGAGTCAAGTTCCACGTCACGGATGCCACCCTCACCCAGTTCAACTTCTCCTCCGACAACAACCTCCACTACAATCTAGCTGCCAACCTCACCATCCGAAACCCCAACAAGAAGATCGGCATCTACTACGATCGCATCGAAGCCAGAGCCCTGTACGAGGGGCAAAGGTTCAGTACGGTTACTCTCACGCCGTTTTACCAAGGCCATAAAACTACAAACGTTCTGAACCCGGTGTTTCAGGGACAACAATTGATTGTTGGATCGAACTTGCTTGAGGAGTTTAATCAACAGAAGAGTGCAGGGGTTTACGAGATTGAGATGAAGCTTTATCTGAGGATTCGGTTCAAGTTGGGTAGAATCAAGACCGGAAAGTTCAAGCCTAGAATTGAGTGTGACTTGAAGGTTCCTTTGAACCAGAATGGGAATTCAGTTGGTACCTTTCAGACTGAGAAGTGCTCCATTGATTACTTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

227

Amino Acids

25.7

Weight (kDa)

9.47

Isoelectric Point (pI)

31.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 99 - 195 1.1e-10 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 641
AccB1I GGYRCC 1 cut(s) 641
AccII CGCG 1 cut(s) 84
AciI CCGC 5 cut(s) 53, 73, 82, 84, 150
AclI AACGTT 1 cut(s) 426
AclWI GGATC 1 cut(s) 475
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 1 cut(s) 631
AcuI CTGAAG 1 cut(s) 106
AfaI GTAC 3 cut(s) 362, 382, 643
AfiI CCNNNNNNNGG 3 cut(s) 83, 226, 364
AgsI TTSAA 8 cut(s) 25, 136, 250, 553, 583, 607, 619, 679
AjiI CACGTC 1 cut(s) 223
AluBI AGCT 5 cut(s) 102, 114, 139, 287, 532
AluI AGCT 5 cut(s) 102, 114, 139, 287, 532
Alw21I GWGCWC 1 cut(s) 665
Alw26I GTCTC 3 cut(s) 56, 98, 131
AlwI GGATC 1 cut(s) 475
AlwNI CAGNNNCTG 1 cut(s) 105
AoxI GGCC 4 cut(s) 40, 79, 200, 409
ApeKI GCWGC 6 cut(s) 102, 105, 108, 111, 114, 287
ApoI RAATTY 1 cut(s) 631
Asp718I GGTACC 1 cut(s) 641
AspS9I GGNCC 1 cut(s) 41
AsuC2I CCSGG 2 cut(s) 78, 437
AsuHPI GGTGA 2 cut(s) 232, 289
BanI GGYRCC 1 cut(s) 641
BanII GRGCYC 1 cut(s) 358
BauI CACGAG 1 cut(s) 57
BbsI GAAGAC 3 cut(s) 124, 151, 175
Bbv12I GWGCWC 1 cut(s) 665
BbvCI CCTCAGC 1 cut(s) 98
BbvI GCAGC 6 cut(s) 89, 92, 95, 101, 123, 274
BccI CCATC 2 cut(s) 52, 308
BceAI ACGGC 2 cut(s) 55, 382
BcnI CCSGG 2 cut(s) 78, 437
BcoDI GTCTC 3 cut(s) 56, 98, 131
BfaI CTAG 2 cut(s) 284, 588
BfmI CTRYAG 1 cut(s) 109
BisI GCNGC 8 cut(s) 82, 103, 106, 109, 112, 115, 150, 288
BlpI GCTNAGC 1 cut(s) 169
BlsI GCNGC 8 cut(s) 83, 104, 107, 110, 113, 116, 151, 289
Bme1390I CCNGG 2 cut(s) 78, 437
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 1 cut(s) 41
BmiI GGNNCC 3 cut(s) 31, 612, 643
BmrFI CCNGG 2 cut(s) 78, 437
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 4 cut(s) 126, 220, 336, 351
BmuI ACTGGG 1 cut(s) 238
BpiI GAAGAC 3 cut(s) 124, 151, 175
BplI GAGNNNNNCTC 4 cut(s) 81, 113, 647, 679
Bpu10I CCTNAGC 1 cut(s) 98
Bpu1102I GCTNAGC 1 cut(s) 169
BpuEI CTTGAG 1 cut(s) 500
BpuMI CCSGG 2 cut(s) 78, 437
BsaBI GATNNNNATC 1 cut(s) 326
BsaI GGTCTC 2 cut(s) 56, 98
BsaJI CCNNGG 3 cut(s) 76, 82, 406
BsaWI WCCGGW 2 cut(s) 206, 572
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 226, 364
Bse1I ACTGG 1 cut(s) 244
Bse8I GATNNNNATC 1 cut(s) 326
BseDI CCNNGG 3 cut(s) 76, 82, 406
BseGI GGATG 3 cut(s) 44, 235, 300
BseJI GATNNNNATC 1 cut(s) 326
BseLI CCNNNNNNNGG 3 cut(s) 83, 226, 364
BseMII CTCAG 3 cut(s) 112, 530, 645
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 3 cut(s) 86, 247, 497
BseX3I CGGCCG 1 cut(s) 79
BseXI GCAGC 6 cut(s) 89, 92, 95, 101, 123, 274
BsgI GTGCAG 1 cut(s) 525
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 2 cut(s) 82, 340
BshFI GGCC 4 cut(s) 42, 81, 202, 411
BshNI GGYRCC 1 cut(s) 641
BsiEI CGRYCG 2 cut(s) 82, 340
BsiHKAI GWGCWC 1 cut(s) 665
BsiSI CCGG 4 cut(s) 78, 207, 437, 573
BslFI GGGAC 2 cut(s) 35, 462
BslI CCNNNNNNNGG 3 cut(s) 83, 226, 364
BsmAI GTCTC 3 cut(s) 56, 98, 131
BsmFI GGGAC 2 cut(s) 35, 462
BsnI GGCC 4 cut(s) 42, 81, 202, 411
Bso31I GGTCTC 2 cut(s) 56, 98
Bsp1286I GDGCHC 2 cut(s) 358, 665
Bsp143I GATC 3 cut(s) 321, 337, 467
Bsp1720I GCTNAGC 1 cut(s) 169
BspACI CCGC 5 cut(s) 53, 73, 82, 84, 150
BspANI GGCC 4 cut(s) 42, 81, 202, 411
BspCNI CTCAG 3 cut(s) 111, 531, 646
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 3 cut(s) 31, 612, 643
BspMAI CTGCAG 1 cut(s) 113
BspPI GGATC 1 cut(s) 475
BspT107I GGYRCC 1 cut(s) 641
BspTNI GGTCTC 2 cut(s) 56, 98
BsrI ACTGG 1 cut(s) 244
BssECI CCNNGG 3 cut(s) 76, 82, 406
BssMI GATC 3 cut(s) 321, 337, 467
BssSI CACGAG 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 406
Bst2BI CACGAG 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 385
Bst6I CTCTTC 1 cut(s) 494
BstDEI CTNAG 4 cut(s) 98, 169, 539, 654
BstDSI CCRYGG 1 cut(s) 82
BstENI CCTNNNNNAGG 1 cut(s) 362
BstF5I GGATG 3 cut(s) 44, 235, 300
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 3 cut(s) 324, 340, 470
BstMAI GTCTC 3 cut(s) 56, 98, 131
BstMBI GATC 3 cut(s) 321, 337, 467
BstMCI CGRYCG 2 cut(s) 82, 340
BstMWI GCNNNNNNNGC 4 cut(s) 81, 108, 111, 114
BstSCI CCNGG 2 cut(s) 76, 435
BstSFI CTRYAG 1 cut(s) 109
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 6 cut(s) 89, 92, 95, 101, 123, 274
BstV2I GAAGAC 3 cut(s) 124, 151, 175
BstXI CCANNNNNNTGG 1 cut(s) 26
BstZI CGGCCG 1 cut(s) 79
BsuRI GGCC 4 cut(s) 42, 81, 202, 411
BtgI CCRYGG 1 cut(s) 82
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 3 cut(s) 44, 235, 300
CaiI CAGNNNCTG 1 cut(s) 105
Cfr13I GGNCC 1 cut(s) 41
Cfr42I CCGCGG 1 cut(s) 85
Csp6I GTAC 3 cut(s) 361, 381, 642
CviQI GTAC 3 cut(s) 361, 381, 642
DdeI CTNAG 4 cut(s) 98, 169, 539, 654
DpnI GATC 3 cut(s) 323, 339, 469
DpnII GATC 3 cut(s) 321, 337, 467
EaeI YGGCCR 1 cut(s) 79
EagI CGGCCG 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
EclXI CGGCCG 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 406
Eco24I GRGCYC 1 cut(s) 358
Eco31I GGTCTC 2 cut(s) 56, 98
Eco52I CGGCCG 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 106
EcoNI CCTNNNNNAGG 1 cut(s) 362
EcoRI GAATTC 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 406
EcoT38I GRGCYC 1 cut(s) 358
ErhI CCWWGG 1 cut(s) 406
FaiI YATR 2 cut(s) 194, 414
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FaqI GGGAC 2 cut(s) 35, 462
FauI CCCGC 1 cut(s) 60
Fnu4HI GCNGC 8 cut(s) 82, 103, 106, 109, 112, 115, 150, 288
FokI GGATG 3 cut(s) 31, 242, 287
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 8 cut(s) 82, 103, 106, 109, 112, 115, 150, 288
FspBI CTAG 2 cut(s) 284, 588
GluI GCNGC 8 cut(s) 82, 103, 106, 109, 112, 115, 150, 288
HaeIII GGCC 4 cut(s) 42, 81, 202, 411
HapII CCGG 4 cut(s) 78, 207, 437, 573
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 3 cut(s) 210, 544, 564
HpaII CCGG 4 cut(s) 78, 207, 437, 573
HphI GGTGA 2 cut(s) 232, 289
Hpy166II GTNNAC 1 cut(s) 514
Hpy188I TCNGA 6 cut(s) 7, 262, 305, 432, 540, 651
Hpy188III TCNNGA 1 cut(s) 568
Hpy8I GTNNAC 1 cut(s) 514
HpyAV CCTTC 2 cut(s) 184, 601
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 2 cut(s) 222, 426
HpyCH4V TGCA 3 cut(s) 111, 117, 506
HpyF10VI GCNNNNNNNGC 4 cut(s) 81, 108, 111, 114
HpyF3I CTNAG 4 cut(s) 98, 169, 539, 654
HpySE526I ACGT 2 cut(s) 222, 426
KpnI GGTACC 1 cut(s) 645
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 3 cut(s) 321, 337, 467
LmnI GCTCC 2 cut(s) 29, 668
Lsp1109I GCAGC 6 cut(s) 89, 92, 95, 101, 123, 274
LweI GCATC 4 cut(s) 126, 220, 336, 351
MaeI CTAG 2 cut(s) 284, 588
MaeII ACGT 2 cut(s) 222, 426
MaeIII GTNAC 3 cut(s) 223, 385, 599
MalI GATC 3 cut(s) 323, 339, 469
MboI GATC 3 cut(s) 321, 337, 467
MboII GAAGA 7 cut(s) 112, 124, 136, 151, 175, 331, 511
MfeI CAATTG 2 cut(s) 455, 679
MhlI GDGCHC 2 cut(s) 358, 665
MluCI AATT 4 cut(s) 455, 591, 631, 679
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 2 cut(s) 285, 445
MseI TTAA 1 cut(s) 489
MslI CAYNNNNRTG 1 cut(s) 24
MspA1I CMGCKG 3 cut(s) 84, 102, 114
MspI CCGG 4 cut(s) 78, 207, 437, 573
MspR9I CCNGG 2 cut(s) 78, 437
MunI CAATTG 2 cut(s) 455, 679
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 4 cut(s) 81, 108, 111, 114
NciI CCSGG 2 cut(s) 78, 437
NdeII GATC 3 cut(s) 321, 337, 467
NlaIV GGNNCC 3 cut(s) 31, 612, 643
NmuCI GTSAC 2 cut(s) 223, 599
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 342
PfeI GAWTC 2 cut(s) 544, 564
PkrI GCNGC 8 cut(s) 83, 104, 107, 110, 113, 116, 151, 289
Ple19I CGATCG 1 cut(s) 340
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Psp1406I AACGTT 1 cut(s) 426
PspN4I GGNNCC 3 cut(s) 31, 612, 643
PspPI GGNCC 1 cut(s) 41
PstI CTGCAG 1 cut(s) 113
PstNI CAGNNNCTG 1 cut(s) 105
PvuI CGATCG 1 cut(s) 340
PvuII CAGCTG 2 cut(s) 102, 114
RsaI GTAC 3 cut(s) 362, 382, 643
RsaNI GTAC 3 cut(s) 361, 381, 642
RseI CAYNNNNRTG 1 cut(s) 24
SacII CCGCGG 1 cut(s) 85
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 8 cut(s) 82, 103, 106, 109, 112, 115, 150, 288
Sau3AI GATC 3 cut(s) 321, 337, 467
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 2 cut(s) 78, 437
SduI GDGCHC 2 cut(s) 358, 665
SfaNI GCATC 4 cut(s) 126, 220, 336, 351
SfcI CTRYAG 1 cut(s) 109
Sfr303I CCGCGG 1 cut(s) 85
SgrBI CCGCGG 1 cut(s) 85
SmiMI CAYNNNNRTG 1 cut(s) 24
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 4 cut(s) 455, 591, 631, 679
SsiI CCGC 5 cut(s) 53, 73, 82, 84, 150
SspMI CTAG 2 cut(s) 284, 588
StyD4I CCNGG 2 cut(s) 76, 435
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 2 cut(s) 225, 429
TaqI TCGA 2 cut(s) 345, 470
TasI AATT 4 cut(s) 455, 591, 631, 679
TauI GCSGC 2 cut(s) 84, 152
TfiI GAWTC 2 cut(s) 544, 564
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TseFI GTSAC 2 cut(s) 223, 599
TseI GCWGC 6 cut(s) 102, 105, 108, 111, 114, 287
Tsp45I GTSAC 2 cut(s) 223, 599
TspDTI ATGAA 1 cut(s) 542
TspGWI ACGGA 2 cut(s) 37, 242
XagI CCTNNNNNAGG 1 cut(s) 362
XapI RAATTY 1 cut(s) 631
XspI CTAG 2 cut(s) 284, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.