Rorug03G0150600

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001177
Physical Location & Seq
Reverse (-)
12419349 .. 12422433
3085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0150600.1

Sequence Viewer

Length: 678 bp
ATGCCGATTCGGAATCAGCTGAATTCCAGGGTTCCGATCAAGTTCCTGGCCTTCTGCGTCTTCCTCAGCTGCTTTCCAGGATGCATACTATCGGCGATTGTGACGCTGGATTCGATTGAGATATACAGCACGCACGAGCTGTTGAAAAGGCCGACGGTTTATTTCAAATGCAAAGGGGATCAGAACAAGACGGTGTTGCCGGACGTCAAGGAAAAGAAGGTCTTGTATAAATTCAACGGCCAAGAGTCTTGGCAGCCTTTGACAGAGCTCGAAGGTAAGAAGTGTAAGCGGTGTGGATTTTATGAGGAGGATACATTCAAATCAGATGACGTATTTGAGGAGTGGGAGTTGTGTCCTTCTGATTTCGATGGTCCTGATGGAAAATTCCGGCGAATCACAAAAGACGAATTCAATGCAACTTTTGTGTGTTCGGAGTGTGACATGACTGGATCCAAATCCAATAGCAAGTCGCATGAAGAAGCAAAGGGAAGGGGATTGCATATTGCTCTAGTTCTATTAATCGTTGCTGTCGTGTTAGCACTGTGTATCGCTGGAGGAGTAGCTGGATACAAGCACTGGCAAAAGAAGAAGCGACAGCAAGAACAGGCTCGGTTCTTAAAGCTGTTTGAAGATGGTGATGAAATTGAGGATGAATTAGGGCTTGATCATGTAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

225

Amino Acids

25.67

Weight (kDa)

6.01

Isoelectric Point (pI)

46.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7953 PF25829 31 - 144 6.4e-54 Domain of unknown function (DUF7953)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 207
AciI CCGC 1 cut(s) 289
AclWI GGATC 3 cut(s) 186, 444, 457
AcoI YGGCCR 1 cut(s) 238
AcsI RAATTY 4 cut(s) 22, 230, 383, 407
AcyI GRCGYC 1 cut(s) 204
AgsI TTSAA 6 cut(s) 145, 166, 235, 319, 412, 629
AjnI CCWGG 3 cut(s) 26, 45, 76
AluBI AGCT 6 cut(s) 19, 69, 139, 268, 563, 622
AluI AGCT 6 cut(s) 19, 69, 139, 268, 563, 622
Alw21I GWGCWC 1 cut(s) 270
AlwI GGATC 3 cut(s) 186, 444, 457
AoxI GGCC 3 cut(s) 48, 149, 238
ApeKI GCWGC 2 cut(s) 69, 253
ApoI RAATTY 4 cut(s) 22, 230, 383, 407
ArsI GACNNNNNNTTYG 2 cut(s) 94, 126
AseI ATTAAT 1 cut(s) 518
AspS9I GGNCC 1 cut(s) 371
AsuHPI GGTGA 1 cut(s) 647
AvaII GGWCC 1 cut(s) 371
BamHI GGATCC 1 cut(s) 449
BanII GRGCYC 1 cut(s) 270
BauI CACGAG 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 52
Bbv12I GWGCWC 1 cut(s) 270
BbvCI CCTCAGC 1 cut(s) 65
BbvI GCAGC 2 cut(s) 56, 265
BccI CCATC 3 cut(s) 362, 371, 626
BceAI ACGGC 1 cut(s) 253
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BciT130I CCWGG 3 cut(s) 28, 47, 78
BciVI GTATCC 2 cut(s) 304, 560
BclI TGATCA 1 cut(s) 664
BfaI CTAG 1 cut(s) 509
BfuI GTATCC 2 cut(s) 304, 560
BisI GCNGC 2 cut(s) 70, 254
BlsI GCNGC 2 cut(s) 71, 255
Bme1390I CCNGG 3 cut(s) 28, 47, 78
Bme18I GGWCC 1 cut(s) 371
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 2 cut(s) 33, 451
BmrFI CCNGG 3 cut(s) 28, 47, 78
BmsI GCATC 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 52
BpmI CTGGAG 1 cut(s) 573
Bpu10I CCTNAGC 1 cut(s) 65
BsaBI GATNNNNATC 1 cut(s) 454
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 27
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bse1I ACTGG 2 cut(s) 451, 581
Bse8I GATNNNNATC 1 cut(s) 454
BseBI CCWGG 3 cut(s) 28, 47, 78
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 2 cut(s) 86, 655
BseJI GATNNNNATC 1 cut(s) 454
BseMII CTCAG 1 cut(s) 79
BseNI ACTGG 2 cut(s) 451, 581
BseRI GAGGAG 3 cut(s) 320, 353, 570
BseXI GCAGC 2 cut(s) 56, 265
BshFI GGCC 3 cut(s) 50, 151, 240
BsiHKAI GWGCWC 1 cut(s) 270
BsiSI CCGG 2 cut(s) 200, 388
BsnI GGCC 3 cut(s) 50, 151, 240
Bsp1286I GDGCHC 1 cut(s) 270
Bsp143I GATC 4 cut(s) 36, 178, 449, 664
BspACI CCGC 1 cut(s) 289
BspANI GGCC 3 cut(s) 50, 151, 240
BspCNI CTCAG 1 cut(s) 78
BspLI GGNNCC 2 cut(s) 33, 451
BspPI GGATC 3 cut(s) 186, 444, 457
BsrI ACTGG 2 cut(s) 451, 581
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 4 cut(s) 36, 178, 449, 664
BssNI GRCGYC 1 cut(s) 204
BssSI CACGAG 1 cut(s) 134
Bst2BI CACGAG 1 cut(s) 134
Bst2UI CCWGG 3 cut(s) 28, 47, 78
Bst4CI ACNGT 3 cut(s) 157, 193, 543
BstACI GRCGYC 1 cut(s) 204
BstC8I GCNNGC 1 cut(s) 131
BstDEI CTNAG 1 cut(s) 65
BstF5I GGATG 2 cut(s) 86, 655
BstKTI GATC 4 cut(s) 39, 181, 452, 667
BstMBI GATC 4 cut(s) 36, 178, 449, 664
BstNI CCWGG 3 cut(s) 28, 47, 78
BstSCI CCNGG 3 cut(s) 26, 45, 76
BstV1I GCAGC 2 cut(s) 56, 265
BstV2I GAAGAC 1 cut(s) 52
BstX2I RGATCY 1 cut(s) 449
BstYI RGATCY 1 cut(s) 449
BsuI GTATCC 2 cut(s) 304, 560
BsuRI GGCC 3 cut(s) 50, 151, 240
BtsCI GGATG 2 cut(s) 86, 655
BtsIMutI CAGTG 2 cut(s) 539, 574
Cac8I GCNNGC 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 371
CseI GACGC 2 cut(s) 46, 112
CviAII CATG 3 cut(s) 442, 473, 668
DdeI CTNAG 1 cut(s) 65
DpnI GATC 4 cut(s) 38, 180, 451, 666
DpnII GATC 4 cut(s) 36, 178, 449, 664
EaeI YGGCCR 1 cut(s) 238
Ecl136II GAGCTC 1 cut(s) 268
Eco24I GRGCYC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 371
Eco53kI GAGCTC 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 268
EcoRI GAATTC 2 cut(s) 22, 407
EcoRII CCWGG 3 cut(s) 26, 45, 76
EcoT22I ATGCAT 1 cut(s) 86
EcoT38I GRGCYC 1 cut(s) 270
FaeI CATG 3 cut(s) 445, 476, 671
FaiI YATR 9 cut(s) 86, 124, 228, 303, 443, 474, 501, 669, 676
FalI AAGNNNNNCTT 2 cut(s) 206, 238
FatI CATG 3 cut(s) 441, 472, 667
FbaI TGATCA 1 cut(s) 664
Fnu4HI GCNGC 2 cut(s) 70, 254
FokI GGATG 2 cut(s) 93, 662
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 2 cut(s) 70, 254
FspBI CTAG 1 cut(s) 509
GluI GCNGC 2 cut(s) 70, 254
GsuI CTGGAG 1 cut(s) 573
HaeIII GGCC 3 cut(s) 50, 151, 240
HapII CCGG 2 cut(s) 200, 388
HgaI GACGC 2 cut(s) 46, 112
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 3 cut(s) 445, 476, 671
HinfI GANTC 5 cut(s) 7, 13, 110, 245, 393
HpaII CCGG 2 cut(s) 200, 388
HphI GGTGA 1 cut(s) 647
Hpy188I TCNGA 6 cut(s) 12, 36, 183, 325, 361, 433
Hpy188III TCNNGA 1 cut(s) 374
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 5 cut(s) 61, 211, 266, 366, 483
HpyCH4III ACNGT 3 cut(s) 157, 193, 543
HpyCH4IV ACGT 2 cut(s) 204, 330
HpyCH4V TGCA 4 cut(s) 84, 171, 416, 499
HpyF3I CTNAG 1 cut(s) 65
HpySE526I ACGT 2 cut(s) 204, 330
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 3 cut(s) 445, 476, 671
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 4 cut(s) 36, 178, 449, 664
Lsp1109I GCAGC 2 cut(s) 56, 265
LweI GCATC 1 cut(s) 71
MaeI CTAG 1 cut(s) 509
MaeII ACGT 2 cut(s) 204, 330
MaeIII GTNAC 2 cut(s) 100, 437
MalI GATC 4 cut(s) 38, 180, 451, 666
MboI GATC 4 cut(s) 36, 178, 449, 664
MboII GAAGA 4 cut(s) 52, 488, 598, 641
MflI RGATCY 1 cut(s) 449
MhlI GDGCHC 1 cut(s) 270
MluCI AATT 6 cut(s) 22, 230, 383, 407, 642, 653
MlyI GAGTC 1 cut(s) 254
MnlI CCTC 6 cut(s) 74, 298, 301, 331, 548, 640
Mph1103I ATGCAT 1 cut(s) 86
MseI TTAA 2 cut(s) 518, 617
MspA1I CMGCKG 2 cut(s) 19, 69
MspI CCGG 2 cut(s) 200, 388
MspR9I CCNGG 3 cut(s) 28, 47, 78
MvaI CCWGG 3 cut(s) 28, 47, 78
NdeII GATC 4 cut(s) 36, 178, 449, 664
NlaIII CATG 3 cut(s) 445, 476, 671
NlaIV GGNNCC 2 cut(s) 33, 451
NmuCI GTSAC 2 cut(s) 100, 437
NsiI ATGCAT 1 cut(s) 86
PcsI WCGNNNNNNNCGW 2 cut(s) 110, 528
PfeI GAWTC 4 cut(s) 7, 13, 110, 393
PfoI TCCNGGA 1 cut(s) 76
PkrI GCNGC 2 cut(s) 71, 255
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
PshBI ATTAAT 1 cut(s) 518
Psp124BI GAGCTC 1 cut(s) 270
Psp6I CCWGG 3 cut(s) 26, 45, 76
PspGI CCWGG 3 cut(s) 26, 45, 76
PspN4I GGNNCC 2 cut(s) 33, 451
PspPI GGNCC 1 cut(s) 371
PsuI RGATCY 1 cut(s) 449
PvuII CAGCTG 2 cut(s) 19, 69
SacI GAGCTC 1 cut(s) 270
SaqAI TTAA 2 cut(s) 518, 617
SatI GCNGC 2 cut(s) 70, 254
Sau3AI GATC 4 cut(s) 36, 178, 449, 664
Sau96I GGNCC 1 cut(s) 371
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 3 cut(s) 28, 47, 78
SduI GDGCHC 1 cut(s) 270
SfaNI GCATC 1 cut(s) 71
SinI GGWCC 1 cut(s) 371
Sse9I AATT 6 cut(s) 22, 230, 383, 407, 642, 653
SsiI CCGC 1 cut(s) 289
SspMI CTAG 1 cut(s) 509
SstI GAGCTC 1 cut(s) 270
StyD4I CCNGG 3 cut(s) 26, 45, 76
TaaI ACNGT 3 cut(s) 157, 193, 543
TaiI ACGT 2 cut(s) 207, 333
TaqI TCGA 3 cut(s) 113, 270, 366
TasI AATT 6 cut(s) 22, 230, 383, 407, 642, 653
TfiI GAWTC 4 cut(s) 7, 13, 110, 393
Tru1I TTAA 2 cut(s) 518, 617
Tru9I TTAA 2 cut(s) 518, 617
TscAI CASTG 2 cut(s) 546, 581
TseFI GTSAC 2 cut(s) 100, 437
TseI GCWGC 2 cut(s) 69, 253
Tsp45I GTSAC 2 cut(s) 100, 437
TspDTI ATGAA 3 cut(s) 489, 654, 666
TspRI CASTG 2 cut(s) 546, 581
VpaK11BI GGWCC 1 cut(s) 371
VspI ATTAAT 1 cut(s) 518
XapI RAATTY 4 cut(s) 22, 230, 383, 407
XspI CTAG 1 cut(s) 509
ZraI GACGTC 1 cut(s) 205
Zsp2I ATGCAT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.