pycom14g03260

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001177
Physical Location & Seq
Reverse (-)
2564412 .. 2565514
1103 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g03260.1

Sequence Viewer

Length: 1020 bp
ATGTTTTATCGGACGATTTTTAGATATAATTTGCAAGGACTACCACGAACCACCTCAATTCCCCAATGCTCACAAATCACCATCCCTCCACACGCTGATAGTCTCTTAAACAAAGACTTCCCACACACACACAAAACGTACCCCTTCTGCCTTCTAAAGCAACCGCGTCGAGTTGATTTTCTCCACCCACCTCCCCACGCGGCCTTGCCACATTCCGCCCTTATATATACTCACACTTCACCTTCTTCACCTCTCATTCTTCTCAGCCACACCCATCTCACTACTCTCTATATCCATTACAAATTGTTTGTTGCTCCTCCGACGTCCTCCGCCATGTCAGAGAAACAAGCCCATTTAAACGGGGCCTACTACGGCCCCTCAATCCCTCCCAAATCCCAGTCCTACCATCGCCCTGGCCGCGGTGGCGGCGGATGCCTCGGCTGCTGCTGCAGCTGCATCTTCGGCCTTGTCTTCAAGCTCATCATGACCGCCGTCGTTTTCATGGGATTGGCGTTCTTCGTCTTTTGGCTCATCGTCCGCCCTAACCGTGTCAAGTTCCACGTCACGGAAGCCACCCTCACGCAGTTCAACTTCTCCAACGACAACACCCTCCACTACAATCTCGCCCTCAACCTCACCATCCGGAACCCTAACAAGAAGATCGGTATTTACTACGACCGCATCGAGACCCGGGCCTTTTACGAGGATCAGAGGTTCAGCACGGTTCCCTCGACGCCTTTCTACCAAGGGCACAAGAAAACGAACGTGCTGAACCCCGTGTTCCAAGGCCAGCAAGTGGTTCAAGGCTCAAAGGTGCTTTCCGAGTACAATCAGCAGAAGAGTACCGGGATTTACGAGATCGATTTGAAAATTTATCTTCGGATTCGGTTCAAATTCGGATGGATCAAGACTGGCAAGTTCAAGCCCAAGATTGAATGCGACTTGAAGGTTCCATTGAATCAGAACGGAAGTTCAGTTGGCACATTCCAGACTACCAAGTGCGACGTTGATTACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

340

Amino Acids

38.7

Weight (kDa)

9.62

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 212 - 310 1.9e-11 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 326
AccB7I CCANNNNNTGG 1 cut(s) 796
AccII CGCG 3 cut(s) 166, 200, 420
AccIII TCCGGA 1 cut(s) 642
AclWI GGATC 2 cut(s) 714, 911
AcoI YGGCCR 1 cut(s) 415
AcsI RAATTY 2 cut(s) 870, 893
AcyI GRCGYC 2 cut(s) 323, 734
AfaI GTAC 3 cut(s) 140, 827, 844
AfiI CCNNNNNNNGG 3 cut(s) 419, 565, 796
AgsI TTSAA 9 cut(s) 475, 589, 803, 868, 892, 922, 935, 946, 958
AjiI CACGTC 1 cut(s) 562
AjnI CCWGG 1 cut(s) 412
AloI GAACNNNNNNTCC 2 cut(s) 698, 730
AluBI AGCT 2 cut(s) 453, 478
AluI AGCT 2 cut(s) 453, 478
Alw26I GTCTC 2 cut(s) 107, 680
AlwI GGATC 2 cut(s) 714, 911
Ama87I CYCGRG 1 cut(s) 690
Aor13HI TCCGGA 1 cut(s) 642
AoxI GGCC 7 cut(s) 201, 363, 373, 415, 463, 693, 787
ApeKI GCWGC 5 cut(s) 441, 444, 447, 450, 453
ApoI RAATTY 2 cut(s) 870, 893
AspS9I GGNCC 3 cut(s) 363, 374, 693
AsuC2I CCSGG 3 cut(s) 691, 692, 847
AsuHPI GGTGA 4 cut(s) 70, 231, 240, 628
AvaI CYCGRG 1 cut(s) 690
BaeGI GKGCMC 1 cut(s) 753
BbsI GAAGAC 1 cut(s) 463
BbvI GCAGC 5 cut(s) 428, 431, 434, 440, 462
BccI CCATC 5 cut(s) 89, 282, 414, 647, 894
BceAI ACGGC 2 cut(s) 388, 476
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BciT130I CCWGG 1 cut(s) 414
BcnI CCSGG 3 cut(s) 691, 692, 847
BcoDI GTCTC 2 cut(s) 107, 680
BfmI CTRYAG 1 cut(s) 448
BglI GCCNNNNNGGC 1 cut(s) 423
BisI GCNGC 8 cut(s) 201, 418, 427, 442, 445, 448, 451, 454
BlsI GCNGC 8 cut(s) 202, 419, 428, 443, 446, 449, 452, 455
Bme1390I CCNGG 4 cut(s) 414, 691, 692, 847
BmeT110I CYCGRG 1 cut(s) 690
BmgBI CACGTC 1 cut(s) 562
BmgT120I GGNCC 3 cut(s) 363, 374, 693
BmiI GGNNCC 5 cut(s) 364, 376, 647, 726, 951
BmrFI CCNGG 4 cut(s) 414, 691, 692, 847
BmrI ACTGGG 1 cut(s) 391
BmsI GCATC 3 cut(s) 422, 465, 690
BmuI ACTGGG 1 cut(s) 391
BoxI GACNNNNGTC 1 cut(s) 491
BpiI GAAGAC 1 cut(s) 463
BpuMI CCSGG 3 cut(s) 691, 692, 847
Bsa29I ATCGAT 1 cut(s) 861
BsaHI GRCGYC 2 cut(s) 323, 734
BsaI GGTCTC 1 cut(s) 680
BsaJI CCNNGG 6 cut(s) 412, 418, 436, 690, 745, 784
BsaWI WCCGGW 1 cut(s) 642
BsaXI ACNNNNNCTCC 2 cut(s) 70, 100
Bsc4I CCNNNNNNNGG 3 cut(s) 419, 565, 796
Bse1I ACTGG 2 cut(s) 397, 916
BseAI TCCGGA 1 cut(s) 642
BseBI CCWGG 1 cut(s) 414
BseCI ATCGAT 1 cut(s) 861
BseDI CCNNGG 6 cut(s) 412, 418, 436, 690, 745, 784
BseGI GGATG 4 cut(s) 81, 437, 639, 905
BseLI CCNNNNNNNGG 3 cut(s) 419, 565, 796
BseMII CTCAG 1 cut(s) 277
BseNI ACTGG 2 cut(s) 397, 916
BseRI GAGGAG 1 cut(s) 306
BseSI GKGCMC 1 cut(s) 753
BseXI GCAGC 5 cut(s) 428, 431, 434, 440, 462
Bsh1236I CGCG 3 cut(s) 166, 200, 420
Bsh1285I CGRYCG 1 cut(s) 679
BshFI GGCC 7 cut(s) 203, 365, 375, 417, 465, 695, 789
BshVI ATCGAT 1 cut(s) 861
BsiEI CGRYCG 1 cut(s) 679
BsiHKCI CYCGRG 1 cut(s) 690
BsiSI CCGG 3 cut(s) 643, 691, 846
BslI CCNNNNNNNGG 3 cut(s) 419, 565, 796
BsmAI GTCTC 2 cut(s) 107, 680
BsmI GAATGC 1 cut(s) 941
BsnI GGCC 7 cut(s) 203, 365, 375, 417, 465, 695, 789
Bso31I GGTCTC 1 cut(s) 680
BsoBI CYCGRG 1 cut(s) 690
Bsp1286I GDGCHC 1 cut(s) 753
Bsp13I TCCGGA 1 cut(s) 642
Bsp143I GATC 4 cut(s) 660, 706, 858, 903
BspANI GGCC 7 cut(s) 203, 365, 375, 417, 465, 695, 789
BspCNI CTCAG 1 cut(s) 276
BspDI ATCGAT 1 cut(s) 861
BspEI TCCGGA 1 cut(s) 642
BspFNI CGCG 3 cut(s) 166, 200, 420
BspHI TCATGA 1 cut(s) 483
BspLI GGNNCC 5 cut(s) 364, 376, 647, 726, 951
BspMAI CTGCAG 1 cut(s) 452
BspPI GGATC 2 cut(s) 714, 911
BspTNI GGTCTC 1 cut(s) 680
BsrI ACTGG 2 cut(s) 397, 916
BssECI CCNNGG 6 cut(s) 412, 418, 436, 690, 745, 784
BssMI GATC 4 cut(s) 660, 706, 858, 903
BssNI GRCGYC 2 cut(s) 323, 734
BssT1I CCWWGG 2 cut(s) 745, 784
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 2 cut(s) 548, 724
Bst6I CTCTTC 1 cut(s) 833
BstACI GRCGYC 2 cut(s) 323, 734
BstC8I GCNNGC 1 cut(s) 791
BstDEI CTNAG 1 cut(s) 263
BstDSI CCRYGG 1 cut(s) 418
BstF5I GGATG 4 cut(s) 81, 437, 639, 905
BstFNI CGCG 3 cut(s) 166, 200, 420
BstKTI GATC 4 cut(s) 663, 709, 861, 906
BstMAI GTCTC 2 cut(s) 107, 680
BstMBI GATC 4 cut(s) 660, 706, 858, 903
BstMCI CGRYCG 1 cut(s) 679
BstMWI GCNNNNNNNGC 9 cut(s) 417, 423, 426, 432, 441, 447, 450, 453, 462
BstNI CCWGG 1 cut(s) 414
BstPAI GACNNNNGTC 1 cut(s) 491
BstSCI CCNGG 4 cut(s) 412, 689, 690, 845
BstSFI CTRYAG 1 cut(s) 448
BstSLI GKGCMC 1 cut(s) 753
BstUI CGCG 3 cut(s) 166, 200, 420
BstV1I GCAGC 5 cut(s) 428, 431, 434, 440, 462
BstV2I GAAGAC 1 cut(s) 463
BstXI CCANNNNNNTGG 1 cut(s) 413
Bsu15I ATCGAT 1 cut(s) 861
BsuRI GGCC 7 cut(s) 203, 365, 375, 417, 465, 695, 789
BsuTUI ATCGAT 1 cut(s) 861
BtgI CCRYGG 1 cut(s) 418
BtgZI GCGATG 1 cut(s) 392
BtrI CACGTC 1 cut(s) 562
BtsCI GGATG 4 cut(s) 81, 437, 639, 905
Cac8I GCNNGC 1 cut(s) 791
CciI TCATGA 1 cut(s) 483
Cfr13I GGNCC 3 cut(s) 363, 374, 693
Cfr42I CCGCGG 1 cut(s) 421
Cfr9I CCCGGG 1 cut(s) 690
ClaI ATCGAT 1 cut(s) 861
CseI GACGC 2 cut(s) 155, 742
Csp6I GTAC 3 cut(s) 139, 826, 843
CviAII CATG 3 cut(s) 334, 484, 502
CviQI GTAC 3 cut(s) 139, 826, 843
DdeI CTNAG 1 cut(s) 263
DpnI GATC 4 cut(s) 662, 708, 860, 905
DpnII GATC 4 cut(s) 660, 706, 858, 903
DraI TTTAAA 1 cut(s) 357
EaeI YGGCCR 1 cut(s) 415
Eam1104I CTCTTC 1 cut(s) 833
EarI CTCTTC 1 cut(s) 833
EciI GGCGGA 4 cut(s) 205, 319, 444, 527
Eco130I CCWWGG 2 cut(s) 745, 784
Eco31I GGTCTC 1 cut(s) 680
Eco88I CYCGRG 1 cut(s) 690
EcoO109I RGGNCCY 1 cut(s) 363
EcoRII CCWGG 1 cut(s) 412
EcoT14I CCWWGG 2 cut(s) 745, 784
ErhI CCWWGG 2 cut(s) 745, 784
FaeI CATG 3 cut(s) 337, 487, 505
FaiI YATR 8 cut(s) 27, 224, 226, 228, 291, 335, 485, 503
FatI CATG 3 cut(s) 333, 483, 501
Fnu4HI GCNGC 8 cut(s) 201, 418, 427, 442, 445, 448, 451, 454
FokI GGATG 4 cut(s) 68, 444, 626, 912
Fsp4HI GCNGC 8 cut(s) 201, 418, 427, 442, 445, 448, 451, 454
GluI GCNGC 8 cut(s) 201, 418, 427, 442, 445, 448, 451, 454
HaeIII GGCC 7 cut(s) 203, 365, 375, 417, 465, 695, 789
HapII CCGG 3 cut(s) 643, 691, 846
HgaI GACGC 2 cut(s) 155, 742
Hin1I GRCGYC 2 cut(s) 323, 734
Hin1II CATG 3 cut(s) 337, 487, 505
HinfI GANTC 2 cut(s) 883, 958
HpaII CCGG 3 cut(s) 643, 691, 846
HphI GGTGA 4 cut(s) 70, 231, 240, 628
Hpy188I TCNGA 8 cut(s) 12, 321, 340, 711, 823, 882, 899, 963
Hpy188III TCNNGA 5 cut(s) 484, 643, 685, 907, 988
Hpy99I CGWCG 5 cut(s) 171, 325, 497, 736, 1007
HpyAV CCTTC 4 cut(s) 154, 161, 252, 940
HpyCH4III ACNGT 2 cut(s) 548, 724
HpyCH4IV ACGT 5 cut(s) 137, 323, 561, 765, 1005
HpyCH4V TGCA 3 cut(s) 34, 450, 456
HpyF10VI GCNNNNNNNGC 9 cut(s) 417, 423, 426, 432, 441, 447, 450, 453, 462
HpyF3I CTNAG 1 cut(s) 263
HpySE526I ACGT 5 cut(s) 137, 323, 561, 765, 1005
Hsp92I GRCGYC 2 cut(s) 323, 734
Hsp92II CATG 3 cut(s) 337, 487, 505
Kpn2I TCCGGA 1 cut(s) 642
KspI CCGCGG 1 cut(s) 421
Kzo9I GATC 4 cut(s) 660, 706, 858, 903
LmnI GCTCC 1 cut(s) 319
LpnPI CCDG 9 cut(s) 399, 410, 426, 656, 704, 803, 859, 897, 1001
Lsp1109I GCAGC 5 cut(s) 428, 431, 434, 440, 462
LweI GCATC 3 cut(s) 422, 465, 690
MaeII ACGT 5 cut(s) 137, 323, 561, 765, 1005
MaeIII GTNAC 1 cut(s) 562
MalI GATC 4 cut(s) 662, 708, 860, 905
MboI GATC 4 cut(s) 660, 706, 858, 903
MboII GAAGA 8 cut(s) 237, 251, 451, 463, 508, 670, 850, 869
MhlI GDGCHC 1 cut(s) 753
MluCI AATT 5 cut(s) 28, 57, 302, 870, 893
MmeI TCCRAC 2 cut(s) 344, 621
MroI TCCGGA 1 cut(s) 642
MseI TTAA 2 cut(s) 107, 356
MspA1I CMGCKG 2 cut(s) 420, 453
MspI CCGG 3 cut(s) 643, 691, 846
MspR9I CCNGG 4 cut(s) 414, 691, 692, 847
Mva1269I GAATGC 1 cut(s) 941
MvaI CCWGG 1 cut(s) 414
MvnI CGCG 3 cut(s) 166, 200, 420
MwoI GCNNNNNNNGC 9 cut(s) 417, 423, 426, 432, 441, 447, 450, 453, 462
NciI CCSGG 3 cut(s) 691, 692, 847
NdeII GATC 4 cut(s) 660, 706, 858, 903
NlaIII CATG 3 cut(s) 337, 487, 505
NlaIV GGNNCC 5 cut(s) 364, 376, 647, 726, 951
NmeAIII GCCGAG 1 cut(s) 417
NmuCI GTSAC 1 cut(s) 562
PagI TCATGA 1 cut(s) 483
PcsI WCGNNNNNNNCGW 2 cut(s) 681, 728
PctI GAATGC 1 cut(s) 941
PfeI GAWTC 2 cut(s) 883, 958
PflMI CCANNNNNTGG 1 cut(s) 796
PkrI GCNGC 8 cut(s) 202, 419, 428, 443, 446, 449, 452, 455
PshAI GACNNNNGTC 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 412
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 5 cut(s) 364, 376, 647, 726, 951
PspPI GGNCC 3 cut(s) 363, 374, 693
PstI CTGCAG 1 cut(s) 452
PvuII CAGCTG 1 cut(s) 453
RsaI GTAC 3 cut(s) 140, 827, 844
RsaNI GTAC 3 cut(s) 139, 826, 843
SacII CCGCGG 1 cut(s) 421
SaqAI TTAA 2 cut(s) 107, 356
SatI GCNGC 8 cut(s) 201, 418, 427, 442, 445, 448, 451, 454
Sau3AI GATC 4 cut(s) 660, 706, 858, 903
Sau96I GGNCC 3 cut(s) 363, 374, 693
ScrFI CCNGG 4 cut(s) 414, 691, 692, 847
SduI GDGCHC 1 cut(s) 753
SfaNI GCATC 3 cut(s) 422, 465, 690
SfcI CTRYAG 1 cut(s) 448
Sfr303I CCGCGG 1 cut(s) 421
SgrBI CCGCGG 1 cut(s) 421
SmaI CCCGGG 1 cut(s) 692
Sse9I AATT 5 cut(s) 28, 57, 302, 870, 893
StyD4I CCNGG 4 cut(s) 412, 689, 690, 845
StyI CCWWGG 2 cut(s) 745, 784
TaaI ACNGT 2 cut(s) 548, 724
TaiI ACGT 5 cut(s) 140, 326, 564, 768, 1008
TaqI TCGA 4 cut(s) 169, 684, 731, 861
TasI AATT 5 cut(s) 28, 57, 302, 870, 893
TatI WGTACW 1 cut(s) 825
TauI GCSGC 3 cut(s) 203, 420, 429
TfiI GAWTC 2 cut(s) 883, 958
Tru1I TTAA 2 cut(s) 107, 356
Tru9I TTAA 2 cut(s) 107, 356
TseFI GTSAC 1 cut(s) 562
TseI GCWGC 5 cut(s) 441, 444, 447, 450, 453
Tsp45I GTSAC 1 cut(s) 562
TspDTI ATGAA 1 cut(s) 490
TspGWI ACGGA 2 cut(s) 581, 981
TspMI CCCGGG 1 cut(s) 690
Van91I CCANNNNNTGG 1 cut(s) 796
XapI RAATTY 2 cut(s) 870, 893
XmaI CCCGGG 1 cut(s) 690
ZraI GACGTC 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.