Rorug03G0150700

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001177
Physical Location & Seq
Forward (+)
12419463 .. 12420062
600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0150700.1

Sequence Viewer

Length: 600 bp
ATGGACAACCAACTCAATCTATATGATGTTGCTGGCGGTGATGGAAATCTTTCATCAGATGGGAGATGGCATCCTTGTCTTTTCTTCAGCCCTAATTACTCCCAGGAGGAATTTCTTCAGGGATTATTATCTTCATCATGTGGGAGGGATGAGGCTGATTTCTCGAGCATTCACTGGAATGAGGATGAACTTGTTGAGGAGACTTATGAGGGAGAAGAACAGTTGCCATATTATGGCAGTGGAAATTGGTTCAGCGAATACAAGCACTCTTTTTGTGACTCTTGGGGAGACGGATACTTCTCCCTTGGGAATGGAATTCAGAAAAATGAAGAAGGCAGTGAGTTGAAGGAGGTAGAAGAAGACTCCGGGCAATGTGAGCAACAGTATGAATATAATTATGGGCAAGATGTCACACAAGAAGACTACCCTACTTCTGATTCGACTACTTGGTCTTGTGATTCTTGGTTTGCCCATGGAGGGAAAACTGATTCCCATGGCTACAATGATCAAGAATCCAGAACTTCACATTCCTACTATCCGCTGGAGATTGGGGTTTATGAAAGTATTTTTGGCTATTGGCCTTGCTTGTATCGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

199

Amino Acids

22.95

Weight (kDa)

4.05

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 448
AccB7I CCANNNNNTGG 1 cut(s) 233
AciI CCGC 2 cut(s) 36, 539
AcsI RAATTY 2 cut(s) 110, 315
AcuI CTGAAG 2 cut(s) 70, 101
AfiI CCNNNNNNNGG 2 cut(s) 233, 477
AgsI TTSAA 1 cut(s) 346
AjnI CCWGG 1 cut(s) 102
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
Alw26I GTCTC 2 cut(s) 194, 282
Ama87I CYCGRG 1 cut(s) 163
AoxI GGCC 1 cut(s) 578
ApoI RAATTY 2 cut(s) 110, 315
Asp700I GAANNNNTTC 2 cut(s) 49, 114
AsuC2I CCSGG 1 cut(s) 367
AsuHPI GGTGA 1 cut(s) 50
AvaI CYCGRG 1 cut(s) 163
BbsI GAAGAC 2 cut(s) 366, 426
BccI CCATC 3 cut(s) 35, 53, 60
BciT130I CCWGG 1 cut(s) 104
BciVI GTATCC 1 cut(s) 287
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 1 cut(s) 367
BcoDI GTCTC 2 cut(s) 194, 282
BfuI GTATCC 1 cut(s) 287
Bme1390I CCNGG 2 cut(s) 104, 367
BmeT110I CYCGRG 1 cut(s) 163
BmrFI CCNGG 2 cut(s) 104, 367
BmsI GCATC 1 cut(s) 79
BpiI GAAGAC 2 cut(s) 366, 426
BpmI CTGGAG 1 cut(s) 563
BpuMI CCSGG 1 cut(s) 367
Bsa29I ATCGAT 1 cut(s) 592
BsaBI GATNNNNATC 2 cut(s) 45, 127
BsaJI CCNNGG 4 cut(s) 102, 304, 472, 493
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 477
Bse1I ACTGG 1 cut(s) 179
Bse3DI GCAATG 1 cut(s) 377
Bse8I GATNNNNATC 2 cut(s) 45, 127
BseBI CCWGG 1 cut(s) 104
BseCI ATCGAT 1 cut(s) 592
BseDI CCNNGG 4 cut(s) 102, 304, 472, 493
BseGI GGATG 3 cut(s) 70, 154, 190
BseJI GATNNNNATC 2 cut(s) 45, 127
BseLI CCNNNNNNNGG 2 cut(s) 233, 477
BseMI GCAATG 1 cut(s) 377
BseNI ACTGG 1 cut(s) 179
BseRI GAGGAG 1 cut(s) 212
BshFI GGCC 1 cut(s) 580
BshVI ATCGAT 1 cut(s) 592
BsiHKCI CYCGRG 1 cut(s) 163
BsiSI CCGG 1 cut(s) 366
BslI CCNNNNNNNGG 2 cut(s) 233, 477
BsmAI GTCTC 2 cut(s) 194, 282
BsmBI CGTCTC 1 cut(s) 282
BsmI GAATGC 1 cut(s) 168
BsnI GGCC 1 cut(s) 580
BsoBI CYCGRG 1 cut(s) 163
Bsp143I GATC 1 cut(s) 505
Bsp19I CCATGG 2 cut(s) 472, 493
BspACI CCGC 2 cut(s) 36, 539
BspANI GGCC 1 cut(s) 580
BspDI ATCGAT 1 cut(s) 592
BsrDI GCAATG 1 cut(s) 377
BsrI ACTGG 1 cut(s) 179
BssECI CCNNGG 4 cut(s) 102, 304, 472, 493
BssMI GATC 1 cut(s) 505
BssT1I CCWWGG 3 cut(s) 304, 472, 493
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 2 cut(s) 222, 384
BstC8I GCNNGC 1 cut(s) 34
BstDSI CCRYGG 2 cut(s) 472, 493
BstF5I GGATG 3 cut(s) 70, 154, 190
BstKTI GATC 1 cut(s) 508
BstMAI GTCTC 2 cut(s) 194, 282
BstMBI GATC 1 cut(s) 505
BstMWI GCNNNNNNNGC 1 cut(s) 376
BstNI CCWGG 1 cut(s) 104
BstSCI CCNGG 2 cut(s) 102, 365
BstV2I GAAGAC 2 cut(s) 366, 426
Bsu15I ATCGAT 1 cut(s) 592
BsuI GTATCC 1 cut(s) 287
BsuRI GGCC 1 cut(s) 580
BsuTUI ATCGAT 1 cut(s) 592
BtgI CCRYGG 2 cut(s) 472, 493
BtsCI GGATG 3 cut(s) 70, 154, 190
BtsI GCAGTG 2 cut(s) 244, 343
BtsIMutI CAGTG 3 cut(s) 172, 244, 343
Cac8I GCNNGC 1 cut(s) 34
ClaI ATCGAT 1 cut(s) 592
CviAII CATG 3 cut(s) 138, 473, 494
CviJI RGCY 5 cut(s) 90, 155, 498, 573, 580
CviKI_1 RGCY 5 cut(s) 90, 155, 498, 573, 580
DpnI GATC 1 cut(s) 507
DpnII GATC 1 cut(s) 505
DrdI GACNNNNNNGTC 1 cut(s) 448
DseDI GACNNNNNNGTC 1 cut(s) 448
Eco130I CCWWGG 3 cut(s) 304, 472, 493
Eco57I CTGAAG 2 cut(s) 70, 101
Eco88I CYCGRG 1 cut(s) 163
EcoRI GAATTC 1 cut(s) 315
EcoRII CCWGG 1 cut(s) 102
EcoT14I CCWWGG 3 cut(s) 304, 472, 493
ErhI CCWWGG 3 cut(s) 304, 472, 493
Esp3I CGTCTC 1 cut(s) 282
FaeI CATG 3 cut(s) 141, 476, 497
FatI CATG 3 cut(s) 137, 472, 493
FbaI TGATCA 1 cut(s) 505
FokI GGATG 3 cut(s) 57, 161, 197
GsuI CTGGAG 1 cut(s) 563
HaeIII GGCC 1 cut(s) 580
HapII CCGG 1 cut(s) 366
Hin1II CATG 3 cut(s) 141, 476, 497
HinfI GANTC 6 cut(s) 278, 362, 437, 458, 488, 512
HpaII CCGG 1 cut(s) 366
HphI GGTGA 1 cut(s) 50
Hpy188I TCNGA 3 cut(s) 58, 321, 436
Hpy188III TCNNGA 3 cut(s) 163, 509, 516
HpyAV CCTTC 2 cut(s) 326, 340
HpyCH4III ACNGT 2 cut(s) 222, 384
HpyF10VI GCNNNNNNNGC 1 cut(s) 376
Hsp92II CATG 3 cut(s) 141, 476, 497
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 1 cut(s) 505
LpnPI CCDG 8 cut(s) 18, 89, 104, 116, 160, 379, 527, 529
LweI GCATC 1 cut(s) 79
MaeIII GTNAC 2 cut(s) 275, 409
MalI GATC 1 cut(s) 507
MboI GATC 1 cut(s) 505
MboII GAAGA 8 cut(s) 76, 107, 123, 227, 341, 368, 371, 431
MluCI AATT 5 cut(s) 94, 110, 244, 315, 394
MlyI GAGTC 2 cut(s) 272, 356
MnlI CCTC 8 cut(s) 100, 138, 145, 175, 190, 202, 343, 470
MroXI GAANNNNTTC 2 cut(s) 49, 114
MslI CAYNNNNRTG 1 cut(s) 177
MspA1I CMGCKG 1 cut(s) 541
MspI CCGG 1 cut(s) 366
MspR9I CCNGG 2 cut(s) 104, 367
Mva1269I GAATGC 1 cut(s) 168
MvaI CCWGG 1 cut(s) 104
MwoI GCNNNNNNNGC 1 cut(s) 376
NciI CCSGG 1 cut(s) 367
NcoI CCATGG 2 cut(s) 472, 493
NdeII GATC 1 cut(s) 505
NlaIII CATG 3 cut(s) 141, 476, 497
NmuCI GTSAC 2 cut(s) 275, 409
PaeR7I CTCGAG 1 cut(s) 163
PctI GAATGC 1 cut(s) 168
PdmI GAANNNNTTC 2 cut(s) 49, 114
PfeI GAWTC 4 cut(s) 437, 458, 488, 512
PflMI CCANNNNNTGG 1 cut(s) 233
PleI GAGTC 2 cut(s) 272, 356
PpsI GAGTC 2 cut(s) 272, 356
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
RseI CAYNNNNRTG 1 cut(s) 177
Sau3AI GATC 1 cut(s) 505
SchI GAGTC 2 cut(s) 272, 356
ScrFI CCNGG 2 cut(s) 104, 367
SetI ASST 1 cut(s) 354
SfaNI GCATC 1 cut(s) 79
Sfr274I CTCGAG 1 cut(s) 163
SlaI CTCGAG 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 177
SmlI CTYRAG 1 cut(s) 163
SmoI CTYRAG 1 cut(s) 163
Sse9I AATT 5 cut(s) 94, 110, 244, 315, 394
SsiI CCGC 2 cut(s) 36, 539
StyD4I CCNGG 2 cut(s) 102, 365
StyI CCWWGG 3 cut(s) 304, 472, 493
TaaI ACNGT 2 cut(s) 222, 384
TaqI TCGA 3 cut(s) 164, 440, 592
TasI AATT 5 cut(s) 94, 110, 244, 315, 394
TfiI GAWTC 4 cut(s) 437, 458, 488, 512
TscAI CASTG 3 cut(s) 179, 244, 343
TseFI GTSAC 2 cut(s) 275, 409
Tsp45I GTSAC 2 cut(s) 275, 409
TspDTI ATGAA 6 cut(s) 42, 123, 201, 342, 402, 573
TspGWI ACGGA 1 cut(s) 306
TspRI CASTG 3 cut(s) 179, 244, 343
Van91I CCANNNNNTGG 1 cut(s) 233
XapI RAATTY 2 cut(s) 110, 315
XhoI CTCGAG 1 cut(s) 163
XmnI GAANNNNTTC 2 cut(s) 49, 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.