RLG00000023826

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001177
Physical Location & Seq
Forward (+)
28793579 .. 28795332
1754 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023826

Sequence Viewer

Length: 987 bp
ATGTCAGAGAAACAAGCCCACTTGAATGGAGCCTATTACGGCCCATCAATCCCGCCGAGAGAGAACTACCACCGCCCCGGCCGCGGCGGAGGTCCCCTCAGCTGCTGCTGTAGCTGCATCTTCGGTCTCGTATTCAAGCTCATCTTCGCCGCAGTTGTCTTCATGGGGTTAGCCTTCCTCGTCTTCTGGCTCATAGTCCGCCCCACCAGAGTCAAGTTCCACGTAACAGATGCCACTCTCACACAATTCAACTTCTCCACCGACAGCAACCTGCACTACAATCTCGCTCTCAACCTCACCATCCGAAACCCCAACAAGAAAATCGGCGTTTACTATGATCGCATCGAAGCCAGAGCTCTTTACGAGGACCAGAGGTTTAGTGCGGTCACTCTCACCCCATTTTACCAAGGCCACAAAACCACCAACGTTTTGAACCCGGTGTTTCAGGGACAGCAATTGATTGTTGGATCCAACTTTCTTGAGGAGTTTAATCAACAGAAGACTGCTGGAGTTTACGAGATCGAGATGAAGCTTTATCTGAGGATTCGGTTCAAGTTGGGTAGAATCAAGACTGGAAAGTTCAAGCCTAGAGTTGAATGTGACTTGAAGGGGACTTCATTTCTTGTTTATTCAGATGCATTGAGAGGCCAGGGTTATTTCCAATCGAAATTATGGATGACAAAAATTGCCACTCTAAAAGTGTTCATACCACAAGTGTATCGAGCGCAAATACTGGAAATACATAGCTTAATAGCCACCGGAGACTGCCTCATGGTTTTCTGGAGCTACGCCGGAGCTCCCAATAAGGAGTGTGACAGATCGCCAGCGTCCATGTCGACTAGAGCCACCTCCAGTGGCCAACAAGATATGGGTGCAAAACACAGAGCCACCTCCAGTGGCCAACAAGATATGGGTGCAAAACAGCCCATAGGTCGACTACAAGACCTTCATAGTGCATGTGAGGAGGACACACACTCTCACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

329

Amino Acids

37.02

Weight (kDa)

9.38

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 99 - 199 4.5e-10 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 279
AccI GTMKAC 2 cut(s) 836, 934
AccII CGCG 1 cut(s) 84
AciI CCGC 8 cut(s) 53, 73, 82, 84, 87, 150, 199, 383
AclI AACGTT 1 cut(s) 426
AclWI GGATC 2 cut(s) 462, 475
AcoI YGGCCR 3 cut(s) 79, 856, 898
AfiI CCNNNNNNNGG 1 cut(s) 83
AgsI TTSAA 8 cut(s) 25, 136, 250, 433, 553, 583, 596, 607
AjnI CCWGG 1 cut(s) 648
AluBI AGCT 8 cut(s) 102, 114, 139, 356, 532, 747, 786, 797
AluI AGCT 8 cut(s) 102, 114, 139, 356, 532, 747, 786, 797
Alw21I GWGCWC 2 cut(s) 358, 799
Alw26I GTCTC 2 cut(s) 131, 756
AlwI GGATC 2 cut(s) 462, 475
AlwNI CAGNNNCTG 1 cut(s) 105
AoxI GGCC 6 cut(s) 40, 79, 409, 646, 856, 898
ApeKI GCWGC 3 cut(s) 102, 105, 114
AspLEI GCGC 1 cut(s) 727
AspS9I GGNCC 3 cut(s) 41, 92, 367
AsuC2I CCSGG 2 cut(s) 78, 437
AsuHPI GGTGA 3 cut(s) 289, 385, 971
AvaII GGWCC 2 cut(s) 92, 367
BaeI ACNNNNGTAYC 2 cut(s) 701, 734
BalI TGGCCA 2 cut(s) 858, 900
BamHI GGATCC 1 cut(s) 467
BanII GRGCYC 2 cut(s) 358, 799
BbsI GAAGAC 3 cut(s) 151, 175, 506
Bbv12I GWGCWC 2 cut(s) 358, 799
BbvCI CCTCAGC 1 cut(s) 98
BbvI GCAGC 3 cut(s) 89, 92, 101
BccI CCATC 2 cut(s) 52, 308
BceAI ACGGC 1 cut(s) 55
BciT130I CCWGG 1 cut(s) 650
BcnI CCSGG 2 cut(s) 78, 437
BcoDI GTCTC 2 cut(s) 131, 756
BfaI CTAG 2 cut(s) 588, 840
BfmI CTRYAG 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 279
BisI GCNGC 6 cut(s) 82, 85, 103, 106, 115, 150
BlsI GCNGC 6 cut(s) 83, 86, 104, 107, 116, 151
Bme1390I CCNGG 3 cut(s) 78, 437, 650
Bme18I GGWCC 2 cut(s) 92, 367
BmgT120I GGNCC 3 cut(s) 41, 92, 367
BmiI GGNNCC 3 cut(s) 31, 94, 469
BmrFI CCNGG 3 cut(s) 78, 437, 650
BmsI GCATC 4 cut(s) 126, 220, 351, 625
BpiI GAAGAC 3 cut(s) 151, 175, 506
BplI GAGNNNNNCTC 4 cut(s) 81, 113, 753, 785
BpmI CTGGAG 4 cut(s) 528, 802, 835, 877
Bpu10I CCTNAGC 1 cut(s) 98
BpuEI CTTGAG 1 cut(s) 500
BpuMI CCSGG 2 cut(s) 78, 437
BsaAI YACGTR 1 cut(s) 223
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 4 cut(s) 76, 82, 406, 649
BsaWI WCCGGW 1 cut(s) 758
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 5 cut(s) 577, 738, 852, 894, 982
BseBI CCWGG 1 cut(s) 650
BseDI CCNNGG 4 cut(s) 76, 82, 406, 649
BseGI GGATG 2 cut(s) 300, 681
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMII CTCAG 2 cut(s) 112, 530
BseNI ACTGG 5 cut(s) 577, 738, 852, 894, 982
BseRI GAGGAG 2 cut(s) 497, 977
BseX3I CGGCCG 1 cut(s) 79
BseXI GCAGC 3 cut(s) 89, 92, 101
BsgI GTGCAG 1 cut(s) 257
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 6 cut(s) 42, 81, 411, 648, 858, 900
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 2 cut(s) 358, 799
BsiSI CCGG 4 cut(s) 78, 437, 759, 792
BslFI GGGAC 3 cut(s) 78, 462, 625
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 2 cut(s) 131, 756
BsmFI GGGAC 3 cut(s) 78, 462, 625
BsnI GGCC 6 cut(s) 42, 81, 411, 648, 858, 900
Bso31I GGTCTC 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 358, 799
Bsp143I GATC 4 cut(s) 337, 467, 519, 818
BspACI CCGC 8 cut(s) 53, 73, 82, 84, 87, 150, 199, 383
BspANI GGCC 6 cut(s) 42, 81, 411, 648, 858, 900
BspCNI CTCAG 2 cut(s) 111, 531
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 3 cut(s) 31, 94, 469
BspMI ACCTGC 1 cut(s) 279
BspPI GGATC 2 cut(s) 462, 475
BspTNI GGTCTC 1 cut(s) 131
BsrI ACTGG 5 cut(s) 577, 738, 852, 894, 982
BssECI CCNNGG 4 cut(s) 76, 82, 406, 649
BssMI GATC 4 cut(s) 337, 467, 519, 818
BssT1I CCWWGG 1 cut(s) 406
Bst2UI CCWGG 1 cut(s) 650
BstBAI YACGTR 1 cut(s) 223
BstC8I GCNNGC 1 cut(s) 825
BstDEI CTNAG 2 cut(s) 98, 539
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 2 cut(s) 300, 681
BstFNI CGCG 1 cut(s) 84
BstHHI GCGC 1 cut(s) 727
BstKTI GATC 4 cut(s) 340, 470, 522, 821
BstMAI GTCTC 2 cut(s) 131, 756
BstMBI GATC 4 cut(s) 337, 467, 519, 818
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 3 cut(s) 81, 111, 114
BstNI CCWGG 1 cut(s) 650
BstNSI RCATGY 1 cut(s) 960
BstSCI CCNGG 3 cut(s) 76, 435, 648
BstSFI CTRYAG 1 cut(s) 109
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 3 cut(s) 89, 92, 101
BstV2I GAAGAC 3 cut(s) 151, 175, 506
BstX2I RGATCY 1 cut(s) 467
BstXI CCANNNNNNTGG 1 cut(s) 26
BstYI RGATCY 1 cut(s) 467
BstZI CGGCCG 1 cut(s) 79
BsuRI GGCC 6 cut(s) 42, 81, 411, 648, 858, 900
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 2 cut(s) 300, 681
BtsIMutI CAGTG 2 cut(s) 859, 901
BveI ACCTGC 1 cut(s) 279
Cac8I GCNNGC 1 cut(s) 825
CaiI CAGNNNCTG 1 cut(s) 105
CfoI GCGC 1 cut(s) 727
Cfr13I GGNCC 3 cut(s) 41, 92, 367
Cfr42I CCGCGG 1 cut(s) 85
CseI GACGC 1 cut(s) 816
CviAII CATG 4 cut(s) 163, 772, 832, 957
DdeI CTNAG 2 cut(s) 98, 539
DpnI GATC 4 cut(s) 339, 469, 521, 820
DpnII GATC 4 cut(s) 337, 467, 519, 818
EaeI YGGCCR 3 cut(s) 79, 856, 898
EagI CGGCCG 1 cut(s) 79
EciI GGCGGA 2 cut(s) 102, 188
Ecl136II GAGCTC 2 cut(s) 356, 797
EclXI CGGCCG 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 406
Eco24I GRGCYC 2 cut(s) 358, 799
Eco31I GGTCTC 1 cut(s) 131
Eco47I GGWCC 2 cut(s) 92, 367
Eco52I CGGCCG 1 cut(s) 79
Eco53kI GAGCTC 2 cut(s) 356, 797
EcoICRI GAGCTC 2 cut(s) 356, 797
EcoO109I RGGNCCY 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 648
EcoT14I CCWWGG 1 cut(s) 406
EcoT22I ATGCAT 1 cut(s) 640
EcoT38I GRGCYC 2 cut(s) 358, 799
ErhI CCWWGG 1 cut(s) 406
FaeI CATG 4 cut(s) 166, 775, 835, 960
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FaqI GGGAC 3 cut(s) 78, 462, 625
FatI CATG 4 cut(s) 162, 771, 831, 956
FauI CCCGC 1 cut(s) 60
FblI GTMKAC 2 cut(s) 836, 934
Fnu4HI GCNGC 6 cut(s) 82, 85, 103, 106, 115, 150
FokI GGATG 2 cut(s) 287, 688
FriOI GRGCYC 2 cut(s) 358, 799
Fsp4HI GCNGC 6 cut(s) 82, 85, 103, 106, 115, 150
FspBI CTAG 2 cut(s) 588, 840
GlaI GCGC 1 cut(s) 726
GluI GCNGC 6 cut(s) 82, 85, 103, 106, 115, 150
GsuI CTGGAG 4 cut(s) 528, 802, 835, 877
HaeIII GGCC 6 cut(s) 42, 81, 411, 648, 858, 900
HapII CCGG 4 cut(s) 78, 437, 759, 792
HgaI GACGC 1 cut(s) 816
HhaI GCGC 1 cut(s) 727
Hin1II CATG 4 cut(s) 166, 775, 835, 960
Hin6I GCGC 1 cut(s) 725
HinP1I GCGC 1 cut(s) 725
HincII GTYRAC 2 cut(s) 837, 935
HindII GTYRAC 2 cut(s) 837, 935
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 3 cut(s) 210, 544, 564
HpaII CCGG 4 cut(s) 78, 437, 759, 792
HphI GGTGA 3 cut(s) 289, 385, 971
Hpy166II GTNNAC 4 cut(s) 331, 514, 837, 935
Hpy188I TCNGA 4 cut(s) 7, 305, 540, 634
Hpy188III TCNNGA 4 cut(s) 479, 523, 568, 781
Hpy8I GTNNAC 4 cut(s) 331, 514, 837, 935
HpyAV CCTTC 3 cut(s) 184, 601, 956
HpyCH4IV ACGT 2 cut(s) 222, 426
HpyCH4V TGCA 6 cut(s) 117, 274, 638, 875, 917, 956
HpyF10VI GCNNNNNNNGC 3 cut(s) 81, 111, 114
HpyF3I CTNAG 2 cut(s) 98, 539
HpySE526I ACGT 2 cut(s) 222, 426
Hsp92II CATG 4 cut(s) 166, 775, 835, 960
HspAI GCGC 1 cut(s) 725
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 4 cut(s) 337, 467, 519, 818
LmnI GCTCC 4 cut(s) 29, 783, 794, 802
Lsp1109I GCAGC 3 cut(s) 89, 92, 101
LweI GCATC 4 cut(s) 126, 220, 351, 625
MaeI CTAG 2 cut(s) 588, 840
MaeII ACGT 2 cut(s) 222, 426
MaeIII GTNAC 4 cut(s) 223, 385, 599, 812
MalI GATC 4 cut(s) 339, 469, 521, 820
MboI GATC 4 cut(s) 337, 467, 519, 818
MboII GAAGA 5 cut(s) 112, 136, 151, 175, 511
MfeI CAATTG 1 cut(s) 455
MflI RGATCY 1 cut(s) 467
MhlI GDGCHC 2 cut(s) 358, 799
MlsI TGGCCA 2 cut(s) 858, 900
MluCI AATT 4 cut(s) 245, 455, 668, 684
MluNI TGGCCA 2 cut(s) 858, 900
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 2 cut(s) 445, 495
Mox20I TGGCCA 2 cut(s) 858, 900
Mph1103I ATGCAT 1 cut(s) 640
MscI TGGCCA 2 cut(s) 858, 900
MseI TTAA 2 cut(s) 489, 749
MslI CAYNNNNRTG 1 cut(s) 24
Msp20I TGGCCA 2 cut(s) 858, 900
MspA1I CMGCKG 2 cut(s) 84, 102
MspI CCGG 4 cut(s) 78, 437, 759, 792
MspR9I CCNGG 3 cut(s) 78, 437, 650
MunI CAATTG 1 cut(s) 455
MvaI CCWGG 1 cut(s) 650
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 3 cut(s) 81, 111, 114
NciI CCSGG 2 cut(s) 78, 437
NdeII GATC 4 cut(s) 337, 467, 519, 818
NlaIII CATG 4 cut(s) 166, 775, 835, 960
NlaIV GGNNCC 3 cut(s) 31, 94, 469
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 3 cut(s) 385, 599, 812
NsiI ATGCAT 1 cut(s) 640
NspI RCATGY 1 cut(s) 960
PfeI GAWTC 2 cut(s) 544, 564
PkrI GCNGC 6 cut(s) 83, 86, 104, 107, 116, 151
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Ppu21I YACGTR 1 cut(s) 223
PpuMI RGGWCCY 1 cut(s) 92
Psp124BI GAGCTC 2 cut(s) 358, 799
Psp1406I AACGTT 1 cut(s) 426
Psp5II RGGWCCY 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 648
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 3 cut(s) 31, 94, 469
PspPI GGNCC 3 cut(s) 41, 92, 367
PspPPI RGGWCCY 1 cut(s) 92
PstNI CAGNNNCTG 1 cut(s) 105
PsuI RGATCY 1 cut(s) 467
PvuII CAGCTG 1 cut(s) 102
RseI CAYNNNNRTG 1 cut(s) 24
SacI GAGCTC 2 cut(s) 358, 799
SacII CCGCGG 1 cut(s) 85
SalI GTCGAC 2 cut(s) 835, 933
SaqAI TTAA 2 cut(s) 489, 749
SatI GCNGC 6 cut(s) 82, 85, 103, 106, 115, 150
Sau3AI GATC 4 cut(s) 337, 467, 519, 818
Sau96I GGNCC 3 cut(s) 41, 92, 367
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 3 cut(s) 78, 437, 650
SduI GDGCHC 2 cut(s) 358, 799
SfaNI GCATC 4 cut(s) 126, 220, 351, 625
SfcI CTRYAG 1 cut(s) 109
Sfr303I CCGCGG 1 cut(s) 85
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 2 cut(s) 92, 367
SmiMI CAYNNNNRTG 1 cut(s) 24
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 4 cut(s) 245, 455, 668, 684
SsiI CCGC 8 cut(s) 53, 73, 82, 84, 87, 150, 199, 383
SspMI CTAG 2 cut(s) 588, 840
SstI GAGCTC 2 cut(s) 358, 799
StyD4I CCNGG 3 cut(s) 76, 435, 648
StyI CCWWGG 1 cut(s) 406
TaiI ACGT 2 cut(s) 225, 429
TaqI TCGA 6 cut(s) 345, 522, 665, 721, 836, 934
TaqII GACCGA 1 cut(s) 113
TasI AATT 4 cut(s) 245, 455, 668, 684
TauI GCSGC 3 cut(s) 84, 87, 152
TfiI GAWTC 2 cut(s) 544, 564
Tru1I TTAA 2 cut(s) 489, 749
Tru9I TTAA 2 cut(s) 489, 749
TscAI CASTG 2 cut(s) 859, 901
TseFI GTSAC 3 cut(s) 385, 599, 812
TseI GCWGC 3 cut(s) 102, 105, 114
Tsp45I GTSAC 3 cut(s) 385, 599, 812
TspDTI ATGAA 5 cut(s) 151, 542, 606, 694, 938
TspRI CASTG 2 cut(s) 859, 901
VpaK11BI GGWCC 2 cut(s) 92, 367
XceI RCATGY 1 cut(s) 960
XmiI GTMKAC 2 cut(s) 836, 934
XspI CTAG 2 cut(s) 588, 840
Zsp2I ATGCAT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.