MD12G1092000.v1.1

Rer1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002176
Physical Location & Seq
Reverse (-)
11608843 .. 11609037
195 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1092000.v1.1.491

Sequence Viewer

Length: 195 bp
ATGCGTGTGTACTATGTCCTGGGGTTCTATGATGTCTCCTATGGCTTGGGGATCTACGTCTTGAATCTCTTGATTGGTTTCTTCTCGCCACAGGTTGACCCGGAGCTTGAGTCCATGGATGGGCCTTCATTGCCAACAAGTGGTTATGATGAGTTTAAGCCTTTTGTTCGCCACCTTCCCGAGTTTAAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006890 GO:0008150 GO:0016192 GO:0048193 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rer1

Protein Analysis

65

Amino Acids

7.4

Weight (kDa)

4.5

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rer1 PF03248 1 - 64 1.1e-21 Rer1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 140
AclWI GGATC 1 cut(s) 59
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 2 cut(s) 120, 140
AgsI TTSAA 1 cut(s) 64
AjnI CCWGG 1 cut(s) 18
AluBI AGCT 1 cut(s) 106
AluI AGCT 1 cut(s) 106
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 1 cut(s) 59
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 122
AsuC2I CCSGG 1 cut(s) 101
AvaI CYCGRG 1 cut(s) 179
BccI CCATC 1 cut(s) 113
BciT130I CCWGG 1 cut(s) 20
BcnI CCSGG 1 cut(s) 101
BcoDI GTCTC 1 cut(s) 40
Bme1390I CCNGG 2 cut(s) 20, 101
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 122
BmrFI CCNGG 2 cut(s) 20, 101
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 101
BsaJI CCNNGG 2 cut(s) 19, 114
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 140
Bse3DI GCAATG 1 cut(s) 128
BseBI CCWGG 1 cut(s) 20
BseDI CCNNGG 2 cut(s) 19, 114
BseGI GGATG 1 cut(s) 124
BseLI CCNNNNNNNGG 2 cut(s) 120, 140
BseMI GCAATG 1 cut(s) 128
BshFI GGCC 1 cut(s) 124
BsiHKCI CYCGRG 1 cut(s) 179
BsiSI CCGG 1 cut(s) 101
BslI CCNNNNNNNGG 2 cut(s) 120, 140
BsmAI GTCTC 1 cut(s) 40
BsnI GGCC 1 cut(s) 124
BsoBI CYCGRG 1 cut(s) 179
Bsp143I GATC 1 cut(s) 51
Bsp19I CCATGG 1 cut(s) 114
BspANI GGCC 1 cut(s) 124
BspPI GGATC 1 cut(s) 59
BsrDI GCAATG 1 cut(s) 128
BssECI CCNNGG 2 cut(s) 19, 114
BssMI GATC 1 cut(s) 51
BssT1I CCWWGG 1 cut(s) 114
Bst2UI CCWGG 1 cut(s) 20
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 1 cut(s) 124
BstKTI GATC 1 cut(s) 54
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 1 cut(s) 51
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstNI CCWGG 1 cut(s) 20
BstSCI CCNGG 2 cut(s) 18, 99
BstX2I RGATCY 1 cut(s) 51
BstYI RGATCY 1 cut(s) 51
BsuRI GGCC 1 cut(s) 124
BtgI CCRYGG 1 cut(s) 114
BtsCI GGATG 1 cut(s) 124
Cfr13I GGNCC 1 cut(s) 122
Csp6I GTAC 1 cut(s) 10
CviAII CATG 1 cut(s) 115
CviJI RGCY 4 cut(s) 45, 106, 124, 160
CviKI_1 RGCY 4 cut(s) 45, 106, 124, 160
CviQI GTAC 1 cut(s) 10
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 114
Eco88I CYCGRG 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 18
EcoT14I CCWWGG 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 1 cut(s) 118
FaiI YATR 5 cut(s) 15, 30, 42, 116, 147
FatI CATG 1 cut(s) 114
FokI GGATG 1 cut(s) 131
HaeIII GGCC 1 cut(s) 124
HapII CCGG 1 cut(s) 101
Hin1II CATG 1 cut(s) 118
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 2 cut(s) 64, 110
HpaII CCGG 1 cut(s) 101
Hpy166II GTNNAC 2 cut(s) 10, 97
Hpy188III TCNNGA 3 cut(s) 61, 70, 179
Hpy8I GTNNAC 2 cut(s) 10, 97
HpyAV CCTTC 2 cut(s) 135, 185
HpyCH4IV ACGT 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 1 cut(s) 118
Kzo9I GATC 1 cut(s) 51
LmnI GCTCC 1 cut(s) 103
LpnPI CCDG 4 cut(s) 5, 32, 77, 114
MaeII ACGT 1 cut(s) 57
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 1 cut(s) 73
MflI RGATCY 1 cut(s) 51
MlyI GAGTC 1 cut(s) 119
MseI TTAA 2 cut(s) 156, 186
MspI CCGG 1 cut(s) 101
MspR9I CCNGG 2 cut(s) 20, 101
MvaI CCWGG 1 cut(s) 20
MwoI GCNNNNNNNGC 1 cut(s) 130
NciI CCSGG 1 cut(s) 101
NcoI CCATGG 1 cut(s) 114
NdeII GATC 1 cut(s) 51
NlaIII CATG 1 cut(s) 118
PfeI GAWTC 1 cut(s) 64
PflMI CCANNNNNTGG 1 cut(s) 140
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
Psp6I CCWGG 1 cut(s) 18
PspGI CCWGG 1 cut(s) 18
PspPI GGNCC 1 cut(s) 122
PsuI RGATCY 1 cut(s) 51
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
SaqAI TTAA 2 cut(s) 156, 186
Sau3AI GATC 1 cut(s) 51
Sau96I GGNCC 1 cut(s) 122
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 2 cut(s) 20, 101
SetI ASST 4 cut(s) 60, 96, 108, 177
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
StyD4I CCNGG 2 cut(s) 18, 99
StyI CCWWGG 1 cut(s) 114
TaiI ACGT 1 cut(s) 60
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 1 cut(s) 64
Tru1I TTAA 2 cut(s) 156, 186
Tru9I TTAA 2 cut(s) 156, 186
TspDTI ATGAA 1 cut(s) 117
Van91I CCANNNNNTGG 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.