MD15G1258000.v1.1

Rer1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002176
Physical Location & Seq
Forward (+)
21940245 .. 21942462
2218 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1258000.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGGGATAGAAAATAATCCGGATTTGATGGAGGGAATTGGAAGTGAGGGCGGAGCAGCGGCGGCGCCACTGGCCCAATGGCGGAGCGATTTTGCGAGAGCATTCCAATACTATTTGGATCGGTCAACGCCTCACACTGTGAATAGATGGCTTGGAACCCTAGTTTTGGCTGTGATTTACATTCTGCGTGTGTACTATGTTCAGGGGTTCTATGTTGTCTCCTATGGCCTGGGGATCTACGTCTTGAATCTCTTGATTGGTTTCTTCTCGCCAAAGGTTGACCCGGAGCTTGAGTCCGTGGATGGGCCTTCATTGCCAACAAGTGGTTCTGATGAGTTTAAGCCTTTTGTTCGCCGCCTTCCCGAGTTTAAGTTTTGGTATTCCATCACAAAGGCATTTGTTGTTGCATTCATCATGACCTTTATTTCTCTGCTGGATGTACCTGTTTTCTGGCCAATACTGCTATGCTACTGGATTGTTCTGTTTGTCCTCACAATGAAACGACAGATCCTACACATGATCAAATACAAATATGTTCCATTTGATATCGGAAAGAGGCGATACACTGGAAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006890 GO:0008150 GO:0016192 GO:0048193 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rer1

Protein Analysis

193

Amino Acids

22.13

Weight (kDa)

9.23

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rer1 PF03248 27 - 189 5.7e-76 Rer1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 64
AccB7I CCANNNNNTGG 1 cut(s) 323
AccIII TCCGGA 1 cut(s) 19
AciI CCGC 5 cut(s) 51, 59, 62, 82, 355
AclWI GGATC 3 cut(s) 126, 242, 502
AcoI YGGCCR 1 cut(s) 452
AcyI GRCGYC 1 cut(s) 65
AdeI CACNNNGTG 1 cut(s) 139
AfaI GTAC 2 cut(s) 194, 441
AfiI CCNNNNNNNGG 4 cut(s) 81, 166, 303, 323
AgsI TTSAA 1 cut(s) 247
AjnI CCWGG 1 cut(s) 228
AjuI GAANNNNNNNTTGG 2 cut(s) 310, 342
AluBI AGCT 1 cut(s) 289
AluI AGCT 1 cut(s) 289
Alw26I GTCTC 1 cut(s) 223
AlwI GGATC 3 cut(s) 126, 242, 502
Ama87I CYCGRG 1 cut(s) 362
Aor13HI TCCGGA 1 cut(s) 19
AoxI GGCC 4 cut(s) 72, 226, 305, 452
ApeKI GCWGC 1 cut(s) 56
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 2 cut(s) 73, 305
AsuC2I CCSGG 1 cut(s) 284
AvaI CYCGRG 1 cut(s) 362
BalI TGGCCA 1 cut(s) 454
BanI GGYRCC 1 cut(s) 64
BbvI GCAGC 1 cut(s) 68
BccI CCATC 4 cut(s) 22, 141, 296, 392
BciT130I CCWGG 1 cut(s) 230
BclI TGATCA 1 cut(s) 519
BcnI CCSGG 1 cut(s) 284
BcoDI GTCTC 1 cut(s) 223
BfaI CTAG 1 cut(s) 161
BfoI RGCGCY 1 cut(s) 68
BisI GCNGC 4 cut(s) 57, 60, 63, 355
BlsI GCNGC 4 cut(s) 58, 61, 64, 356
Bme1390I CCNGG 2 cut(s) 230, 284
BmeT110I CYCGRG 1 cut(s) 362
BmgT120I GGNCC 2 cut(s) 73, 305
BmiI GGNNCC 2 cut(s) 66, 157
BmrFI CCNGG 2 cut(s) 230, 284
BpuEI CTTGAG 1 cut(s) 311
BpuMI CCSGG 1 cut(s) 284
BsaHI GRCGYC 1 cut(s) 65
BsaJI CCNNGG 2 cut(s) 229, 297
BsaWI WCCGGW 1 cut(s) 19
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 4 cut(s) 81, 166, 303, 323
Bse1I ACTGG 3 cut(s) 75, 476, 571
Bse3DI GCAATG 1 cut(s) 311
BseAI TCCGGA 1 cut(s) 19
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 229, 297
BseGI GGATG 2 cut(s) 307, 442
BseLI CCNNNNNNNGG 4 cut(s) 81, 166, 303, 323
BseMI GCAATG 1 cut(s) 311
BseNI ACTGG 3 cut(s) 75, 476, 571
BseXI GCAGC 1 cut(s) 68
BshFI GGCC 4 cut(s) 74, 228, 307, 454
BshNI GGYRCC 1 cut(s) 64
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 2 cut(s) 20, 284
BslI CCNNNNNNNGG 4 cut(s) 81, 166, 303, 323
BsmAI GTCTC 1 cut(s) 223
BsmI GAATGC 2 cut(s) 101, 407
BsnI GGCC 4 cut(s) 74, 228, 307, 454
BsoBI CYCGRG 1 cut(s) 362
Bsp13I TCCGGA 1 cut(s) 19
Bsp143I GATC 4 cut(s) 118, 234, 507, 519
BspACI CCGC 5 cut(s) 51, 59, 62, 82, 355
BspANI GGCC 4 cut(s) 74, 228, 307, 454
BspEI TCCGGA 1 cut(s) 19
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 2 cut(s) 66, 157
BspPI GGATC 3 cut(s) 126, 242, 502
BspT107I GGYRCC 1 cut(s) 64
BsrDI GCAATG 1 cut(s) 311
BsrI ACTGG 3 cut(s) 75, 476, 571
BssECI CCNNGG 2 cut(s) 229, 297
BssMI GATC 4 cut(s) 118, 234, 507, 519
BssNI GRCGYC 1 cut(s) 65
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 139
BstACI GRCGYC 1 cut(s) 65
BstDSI CCRYGG 1 cut(s) 297
BstF5I GGATG 2 cut(s) 307, 442
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 4 cut(s) 121, 237, 510, 522
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 4 cut(s) 118, 234, 507, 519
BstMWI GCNNNNNNNGC 4 cut(s) 62, 71, 313, 460
BstNI CCWGG 1 cut(s) 230
BstSCI CCNGG 2 cut(s) 228, 282
BstV1I GCAGC 1 cut(s) 68
BstX2I RGATCY 2 cut(s) 234, 507
BstYI RGATCY 2 cut(s) 234, 507
BsuRI GGCC 4 cut(s) 74, 228, 307, 454
BtgI CCRYGG 1 cut(s) 297
BtsCI GGATG 2 cut(s) 307, 442
BtsIMutI CAGTG 3 cut(s) 68, 135, 564
CciI TCATGA 1 cut(s) 414
CfoI GCGC 1 cut(s) 67
Cfr13I GGNCC 2 cut(s) 73, 305
Csp6I GTAC 2 cut(s) 193, 440
CviAII CATG 2 cut(s) 415, 517
CviJI RGCY 8 cut(s) 74, 151, 170, 228, 289, 307, 343, 454
CviKI_1 RGCY 8 cut(s) 74, 151, 170, 228, 289, 307, 343, 454
CviQI GTAC 2 cut(s) 193, 440
DinI GGCGCC 1 cut(s) 66
DpnI GATC 4 cut(s) 120, 236, 509, 521
DpnII GATC 4 cut(s) 118, 234, 507, 519
DraIII CACNNNGTG 1 cut(s) 139
EaeI YGGCCR 1 cut(s) 452
EciI GGCGGA 2 cut(s) 66, 97
Eco32I GATATC 1 cut(s) 547
Eco88I CYCGRG 1 cut(s) 362
EcoRII CCWGG 1 cut(s) 228
EcoRV GATATC 1 cut(s) 547
EgeI GGCGCC 1 cut(s) 66
EheI GGCGCC 1 cut(s) 66
FaeI CATG 2 cut(s) 418, 520
FaiI YATR 7 cut(s) 198, 213, 225, 416, 466, 518, 534
FatI CATG 2 cut(s) 414, 516
FbaI TGATCA 1 cut(s) 519
Fnu4HI GCNGC 4 cut(s) 57, 60, 63, 355
FokI GGATG 2 cut(s) 314, 449
Fsp4HI GCNGC 4 cut(s) 57, 60, 63, 355
FspBI CTAG 1 cut(s) 161
GlaI GCGC 1 cut(s) 66
GluI GCNGC 4 cut(s) 57, 60, 63, 355
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 4 cut(s) 74, 228, 307, 454
HapII CCGG 2 cut(s) 20, 284
HhaI GCGC 1 cut(s) 67
Hin1I GRCGYC 1 cut(s) 65
Hin1II CATG 2 cut(s) 418, 520
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HincII GTYRAC 2 cut(s) 126, 280
HindII GTYRAC 2 cut(s) 126, 280
HinfI GANTC 2 cut(s) 247, 293
HpaII CCGG 2 cut(s) 20, 284
Hpy166II GTNNAC 3 cut(s) 126, 193, 280
Hpy188I TCNGA 2 cut(s) 331, 551
Hpy188III TCNNGA 5 cut(s) 20, 244, 253, 362, 415
Hpy8I GTNNAC 3 cut(s) 126, 193, 280
HpyAV CCTTC 2 cut(s) 318, 368
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 1 cut(s) 407
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 71, 313, 460
HpySE526I ACGT 1 cut(s) 240
Hsp92I GRCGYC 1 cut(s) 65
Hsp92II CATG 2 cut(s) 418, 520
HspAI GCGC 1 cut(s) 65
KasI GGCGCC 1 cut(s) 64
Kpn2I TCCGGA 1 cut(s) 19
Ksp22I TGATCA 1 cut(s) 519
Kzo9I GATC 4 cut(s) 118, 234, 507, 519
LmnI GCTCC 3 cut(s) 53, 84, 286
Lsp1109I GCAGC 1 cut(s) 68
MaeI CTAG 1 cut(s) 161
MaeII ACGT 1 cut(s) 240
MalI GATC 4 cut(s) 120, 236, 509, 521
MboI GATC 4 cut(s) 118, 234, 507, 519
MboII GAAGA 1 cut(s) 256
MflI RGATCY 2 cut(s) 234, 507
MlsI TGGCCA 1 cut(s) 454
MluCI AATT 1 cut(s) 36
MluNI TGGCCA 1 cut(s) 454
Mly113I GGCGCC 1 cut(s) 65
MlyI GAGTC 1 cut(s) 302
MnlI CCTC 5 cut(s) 25, 40, 141, 500, 549
Mox20I TGGCCA 1 cut(s) 454
MroI TCCGGA 1 cut(s) 19
MscI TGGCCA 1 cut(s) 454
MseI TTAA 2 cut(s) 339, 369
Msp20I TGGCCA 1 cut(s) 454
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 2 cut(s) 20, 284
MspR9I CCNGG 2 cut(s) 230, 284
Mva1269I GAATGC 2 cut(s) 101, 407
MvaI CCWGG 1 cut(s) 230
MwoI GCNNNNNNNGC 4 cut(s) 62, 71, 313, 460
NarI GGCGCC 1 cut(s) 65
NciI CCSGG 1 cut(s) 284
NdeII GATC 4 cut(s) 118, 234, 507, 519
NlaIII CATG 2 cut(s) 418, 520
NlaIV GGNNCC 2 cut(s) 66, 157
PagI TCATGA 1 cut(s) 414
PctI GAATGC 2 cut(s) 101, 407
PfeI GAWTC 1 cut(s) 247
PflMI CCANNNNNTGG 1 cut(s) 323
PkrI GCNGC 4 cut(s) 58, 61, 64, 356
PleI GAGTC 1 cut(s) 301
PluTI GGCGCC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 301
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 2 cut(s) 66, 157
PspPI GGNCC 2 cut(s) 73, 305
PsuI RGATCY 2 cut(s) 234, 507
RsaI GTAC 2 cut(s) 194, 441
RsaNI GTAC 2 cut(s) 193, 440
SaqAI TTAA 2 cut(s) 339, 369
SatI GCNGC 4 cut(s) 57, 60, 63, 355
Sau3AI GATC 4 cut(s) 118, 234, 507, 519
Sau96I GGNCC 2 cut(s) 73, 305
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 2 cut(s) 230, 284
SetI ASST 5 cut(s) 243, 279, 291, 422, 445
SfoI GGCGCC 1 cut(s) 66
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 1 cut(s) 36
SsiI CCGC 5 cut(s) 51, 59, 62, 82, 355
SspDI GGCGCC 1 cut(s) 64
SspMI CTAG 1 cut(s) 161
StyD4I CCNGG 2 cut(s) 228, 282
TaaI ACNGT 1 cut(s) 139
TaiI ACGT 1 cut(s) 243
TaqII GACCGA 1 cut(s) 111
TasI AATT 1 cut(s) 36
TatI WGTACW 1 cut(s) 192
TauI GCSGC 3 cut(s) 62, 65, 357
TfiI GAWTC 1 cut(s) 247
Tru1I TTAA 2 cut(s) 339, 369
Tru9I TTAA 2 cut(s) 339, 369
TscAI CASTG 3 cut(s) 75, 142, 571
TseI GCWGC 1 cut(s) 56
TspDTI ATGAA 3 cut(s) 300, 400, 512
TspGWI ACGGA 1 cut(s) 286
TspRI CASTG 3 cut(s) 75, 142, 571
Van91I CCANNNNNTGG 1 cut(s) 323
XcmI CCANNNNNNNNNTGG 1 cut(s) 75
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.