MD15G1257900.v1.1

Rer1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002176
Physical Location & Seq
Forward (+)
21935715 .. 21938279
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1257900.v1.1.491

Sequence Viewer

Length: 552 bp
ATGGAGGGACTTGGAAGCGAGGGCGGAGCAGCGGCGGCGCCACTGGCCCAATGGCGGAGCGATTTTGCGAGAGCATTCCAGTACTATTTGGATCGGTCAACGCCTCACACTGTGAATAGGTGGCTAGGAACCCTAGTTGTGGCAATGATTTACATTCTGCGTGTGTACTATGTTCAGGGGTTCTATGTTGTCTCCTATGGTCTGGGGATCTACATCTTGAATCTCTTGATCGGTTTCTTCTCCCCGAAGGTTGATCCGGAGCTTGAGTCCTTGGATGGCGCTTCATTGCCAACAAGTGGTTCTGATGAGTTTAAGCCTTTTGTCCGCCGCCTTCCTGAGTTTAAGTTCTGGTATTCCATCACAAAGGCATTTGTTATTGCATTCATCATGACCTTTATTTCTCTGCTGGATGTACCCGTTTTCTGGCCAATACTGCTATGCTACTGGATTGTTCTGTTTGTCCTCACAATGAAACGACAAATCCTACACATGATCAAATACAAATATGTCCCGTTTGATATCGGAAAGAGGCGGTACTCTGGAAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006890 GO:0008150 GO:0016192 GO:0048193 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rer1

Protein Analysis

184

Amino Acids

21.17

Weight (kDa)

9.47

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rer1 PF03248 18 - 180 5.6e-76 Rer1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 37
AccB7I CCANNNNNTGG 1 cut(s) 296
AccIII TCCGGA 1 cut(s) 256
AciI CCGC 7 cut(s) 24, 32, 35, 55, 325, 328, 532
AclWI GGATC 3 cut(s) 99, 215, 248
AcoI YGGCCR 1 cut(s) 425
AcyI GRCGYC 1 cut(s) 38
AdeI CACNNNGTG 1 cut(s) 112
AfaI GTAC 4 cut(s) 83, 167, 414, 536
AfiI CCNNNNNNNGG 4 cut(s) 54, 139, 296, 423
AgsI TTSAA 1 cut(s) 220
AjuI GAANNNNNNNTTGG 2 cut(s) 283, 315
AluBI AGCT 1 cut(s) 262
AluI AGCT 1 cut(s) 262
Alw26I GTCTC 1 cut(s) 196
AlwI GGATC 3 cut(s) 99, 215, 248
Aor13HI TCCGGA 1 cut(s) 256
AoxI GGCC 2 cut(s) 45, 425
ApeKI GCWGC 1 cut(s) 29
AspLEI GCGC 2 cut(s) 40, 281
AspS9I GGNCC 1 cut(s) 46
BalI TGGCCA 1 cut(s) 427
BanI GGYRCC 1 cut(s) 37
BbvI GCAGC 1 cut(s) 41
BccI CCATC 2 cut(s) 269, 365
BclI TGATCA 1 cut(s) 492
BcoDI GTCTC 1 cut(s) 196
BfaI CTAG 2 cut(s) 125, 134
BfoI RGCGCY 2 cut(s) 41, 282
BisI GCNGC 4 cut(s) 30, 33, 36, 328
BlsI GCNGC 4 cut(s) 31, 34, 37, 329
BmcAI AGTACT 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 2 cut(s) 39, 130
BpuEI CTTGAG 1 cut(s) 284
BsaBI GATNNNNATC 1 cut(s) 212
BsaHI GRCGYC 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 270
BsaWI WCCGGW 1 cut(s) 256
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bsc4I CCNNNNNNNGG 4 cut(s) 54, 139, 296, 423
Bse1I ACTGG 3 cut(s) 48, 79, 449
Bse3DI GCAATG 2 cut(s) 150, 284
Bse8I GATNNNNATC 1 cut(s) 212
BseAI TCCGGA 1 cut(s) 256
BseDI CCNNGG 1 cut(s) 270
BseGI GGATG 2 cut(s) 280, 415
BseJI GATNNNNATC 1 cut(s) 212
BseLI CCNNNNNNNGG 4 cut(s) 54, 139, 296, 423
BseMI GCAATG 2 cut(s) 150, 284
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 3 cut(s) 48, 79, 449
BseXI GCAGC 1 cut(s) 41
BshFI GGCC 2 cut(s) 47, 427
BshNI GGYRCC 1 cut(s) 37
BsiSI CCGG 1 cut(s) 257
BslFI GGGAC 2 cut(s) 21, 494
BslI CCNNNNNNNGG 4 cut(s) 54, 139, 296, 423
BsmAI GTCTC 1 cut(s) 196
BsmFI GGGAC 2 cut(s) 21, 494
BsmI GAATGC 2 cut(s) 74, 380
BsnI GGCC 2 cut(s) 47, 427
Bsp13I TCCGGA 1 cut(s) 256
Bsp143I GATC 5 cut(s) 91, 207, 228, 253, 492
BspACI CCGC 7 cut(s) 24, 32, 35, 55, 325, 328, 532
BspANI GGCC 2 cut(s) 47, 427
BspCNI CTCAG 1 cut(s) 328
BspEI TCCGGA 1 cut(s) 256
BspHI TCATGA 1 cut(s) 387
BspLI GGNNCC 2 cut(s) 39, 130
BspPI GGATC 3 cut(s) 99, 215, 248
BspT107I GGYRCC 1 cut(s) 37
BsrDI GCAATG 2 cut(s) 150, 284
BsrI ACTGG 3 cut(s) 48, 79, 449
BssECI CCNNGG 1 cut(s) 270
BssMI GATC 5 cut(s) 91, 207, 228, 253, 492
BssNI GRCGYC 1 cut(s) 38
BssT1I CCWWGG 1 cut(s) 270
Bst4CI ACNGT 1 cut(s) 112
BstACI GRCGYC 1 cut(s) 38
BstDEI CTNAG 1 cut(s) 336
BstF5I GGATG 2 cut(s) 280, 415
BstH2I RGCGCY 2 cut(s) 41, 282
BstHHI GCGC 2 cut(s) 40, 281
BstKTI GATC 5 cut(s) 94, 210, 231, 256, 495
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 5 cut(s) 91, 207, 228, 253, 492
BstMWI GCNNNNNNNGC 3 cut(s) 35, 44, 433
BstV1I GCAGC 1 cut(s) 41
BstX2I RGATCY 1 cut(s) 207
BstYI RGATCY 1 cut(s) 207
BsuRI GGCC 2 cut(s) 47, 427
BtsCI GGATG 2 cut(s) 280, 415
BtsIMutI CAGTG 2 cut(s) 41, 108
CciI TCATGA 1 cut(s) 387
CfoI GCGC 2 cut(s) 40, 281
Cfr13I GGNCC 1 cut(s) 46
Csp6I GTAC 4 cut(s) 82, 166, 413, 535
CviAII CATG 2 cut(s) 388, 490
CviJI RGCY 5 cut(s) 47, 124, 262, 316, 427
CviKI_1 RGCY 5 cut(s) 47, 124, 262, 316, 427
CviQI GTAC 4 cut(s) 82, 166, 413, 535
DdeI CTNAG 1 cut(s) 336
DinI GGCGCC 1 cut(s) 39
DpnI GATC 5 cut(s) 93, 209, 230, 255, 494
DpnII GATC 5 cut(s) 91, 207, 228, 253, 492
DraIII CACNNNGTG 1 cut(s) 112
EaeI YGGCCR 1 cut(s) 425
EciI GGCGGA 3 cut(s) 39, 70, 314
Eco130I CCWWGG 1 cut(s) 270
Eco32I GATATC 1 cut(s) 520
EcoRV GATATC 1 cut(s) 520
EcoT14I CCWWGG 1 cut(s) 270
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 270
FaeI CATG 2 cut(s) 391, 493
FaiI YATR 7 cut(s) 171, 186, 198, 389, 439, 491, 507
FaqI GGGAC 2 cut(s) 21, 494
FatI CATG 2 cut(s) 387, 489
FbaI TGATCA 1 cut(s) 492
Fnu4HI GCNGC 4 cut(s) 30, 33, 36, 328
FokI GGATG 2 cut(s) 287, 422
Fsp4HI GCNGC 4 cut(s) 30, 33, 36, 328
FspBI CTAG 2 cut(s) 125, 134
GlaI GCGC 2 cut(s) 39, 280
GluI GCNGC 4 cut(s) 30, 33, 36, 328
HaeII RGCGCY 2 cut(s) 41, 282
HaeIII GGCC 2 cut(s) 47, 427
HapII CCGG 1 cut(s) 257
HhaI GCGC 2 cut(s) 40, 281
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 2 cut(s) 391, 493
Hin6I GCGC 2 cut(s) 38, 279
HinP1I GCGC 2 cut(s) 38, 279
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HinfI GANTC 2 cut(s) 220, 266
HpaII CCGG 1 cut(s) 257
Hpy166II GTNNAC 2 cut(s) 99, 166
Hpy188I TCNGA 2 cut(s) 304, 524
Hpy188III TCNNGA 6 cut(s) 217, 226, 257, 335, 388, 540
Hpy8I GTNNAC 2 cut(s) 99, 166
HpyAV CCTTC 2 cut(s) 241, 341
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4V TGCA 1 cut(s) 380
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 44, 433
HpyF3I CTNAG 1 cut(s) 336
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 2 cut(s) 391, 493
HspAI GCGC 2 cut(s) 38, 279
KasI GGCGCC 1 cut(s) 37
Kpn2I TCCGGA 1 cut(s) 256
Ksp22I TGATCA 1 cut(s) 492
Kzo9I GATC 5 cut(s) 91, 207, 228, 253, 492
LmnI GCTCC 3 cut(s) 26, 57, 259
Lsp1109I GCAGC 1 cut(s) 41
MaeI CTAG 2 cut(s) 125, 134
MalI GATC 5 cut(s) 93, 209, 230, 255, 494
MboI GATC 5 cut(s) 91, 207, 228, 253, 492
MboII GAAGA 1 cut(s) 229
MflI RGATCY 1 cut(s) 207
MlsI TGGCCA 1 cut(s) 427
MluNI TGGCCA 1 cut(s) 427
Mly113I GGCGCC 1 cut(s) 38
MlyI GAGTC 1 cut(s) 275
MnlI CCTC 4 cut(s) 13, 114, 473, 522
Mox20I TGGCCA 1 cut(s) 427
MroI TCCGGA 1 cut(s) 256
MscI TGGCCA 1 cut(s) 427
MseI TTAA 2 cut(s) 312, 342
Msp20I TGGCCA 1 cut(s) 427
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 1 cut(s) 257
Mva1269I GAATGC 2 cut(s) 74, 380
MwoI GCNNNNNNNGC 3 cut(s) 35, 44, 433
NarI GGCGCC 1 cut(s) 38
NdeII GATC 5 cut(s) 91, 207, 228, 253, 492
NlaIII CATG 2 cut(s) 391, 493
NlaIV GGNNCC 2 cut(s) 39, 130
PagI TCATGA 1 cut(s) 387
PctI GAATGC 2 cut(s) 74, 380
PfeI GAWTC 1 cut(s) 220
PflMI CCANNNNNTGG 1 cut(s) 296
PkrI GCNGC 4 cut(s) 31, 34, 37, 329
PleI GAGTC 1 cut(s) 274
PluTI GGCGCC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 274
PspN4I GGNNCC 2 cut(s) 39, 130
PspPI GGNCC 1 cut(s) 46
PsuI RGATCY 1 cut(s) 207
RsaI GTAC 4 cut(s) 83, 167, 414, 536
RsaNI GTAC 4 cut(s) 82, 166, 413, 535
SaqAI TTAA 2 cut(s) 312, 342
SatI GCNGC 4 cut(s) 30, 33, 36, 328
Sau3AI GATC 5 cut(s) 91, 207, 228, 253, 492
Sau96I GGNCC 1 cut(s) 46
ScaI AGTACT 1 cut(s) 83
SchI GAGTC 1 cut(s) 275
SetI ASST 4 cut(s) 122, 252, 264, 395
SfoI GGCGCC 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
SsiI CCGC 7 cut(s) 24, 32, 35, 55, 325, 328, 532
SspDI GGCGCC 1 cut(s) 37
SspMI CTAG 2 cut(s) 125, 134
StyI CCWWGG 1 cut(s) 270
TaaI ACNGT 1 cut(s) 112
TaqII GACCGA 1 cut(s) 84
TatI WGTACW 2 cut(s) 81, 165
TauI GCSGC 3 cut(s) 35, 38, 330
TfiI GAWTC 1 cut(s) 220
Tru1I TTAA 2 cut(s) 312, 342
Tru9I TTAA 2 cut(s) 312, 342
TscAI CASTG 2 cut(s) 48, 115
TseI GCWGC 1 cut(s) 29
TspDTI ATGAA 3 cut(s) 273, 373, 485
TspRI CASTG 2 cut(s) 48, 115
Van91I CCANNNNNTGG 1 cut(s) 296
XcmI CCANNNNNNNNNTGG 1 cut(s) 48
XspI CTAG 2 cut(s) 125, 134
ZrmI AGTACT 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.