pycom15g22560

Rer1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002176
Physical Location & Seq
Forward (+)
16682839 .. 16684299
1461 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g22560.3

Sequence Viewer

Length: 660 bp
ATGGAGGGAATTGGAAGTGAGGGCGGAGCAGCGGCGGCGCCACTGGCCCAATGGCGGAGCGATTTTGCGAGAGCATTCCAGTACTATTTGGATCGGTCAACGCCTCACACTGTGAATAGATGGCTTGGAACCCTAGTTTTGGCTATGATTTACATTCTGCGTGTGTACCATGTTCAGGGGTTCTATGTTGTCTCCTATGGCCTGGGGATCTACGTCTTGAATCTCTTGATTGGTTTCTTCTCGCCGAAGGTTGACCCGGAGCTTGAGTCCGTGGATGGGCCTTCATTGCCAACAAGTGGTTCTGATGAGTTTAAGCCTTTTGTTCGCCGCCTTCCCGAGTTTAAGTTCTGGTATTCCATCACAAAGGCATTTGTTGTTGCATTCATCATGACCTTTATTTCTCTGCTGGATGTACCTGTTTTCTGGCCAATACTGCTATGCTACTGGATTGTTCTGTTTGCCCTCACAATGAAACGGCAGATCCTACACATGATCAAATACAAATATGTTCCATTTGATATCGGGAAGAGGGTGAGTAAACATTTCTCATTCTTTCTCCCCATCTTTGTTGTAGGCCCTCGTATCGATACACTGGAAAAAAGTAGTCTGAATGGGAGCGGCTTTTCTACAAATCTGAAATCAAATTCTGCTGATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006890 GO:0008150 GO:0016192 GO:0048193 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rer1

Protein Analysis

220

Amino Acids

24.93

Weight (kDa)

9.25

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 37
AccB7I CCANNNNNTGG 1 cut(s) 296
AccBSI CCGCTC 1 cut(s) 618
AciI CCGC 6 cut(s) 24, 32, 35, 55, 328, 618
AclWI GGATC 3 cut(s) 99, 215, 475
AcoI YGGCCR 1 cut(s) 425
AcsI RAATTY 1 cut(s) 643
AcyI GRCGYC 1 cut(s) 38
AdeI CACNNNGTG 1 cut(s) 112
AfaI GTAC 3 cut(s) 83, 167, 414
AfiI CCNNNNNNNGG 5 cut(s) 54, 139, 175, 276, 296
AgsI TTSAA 1 cut(s) 220
AjnI CCWGG 1 cut(s) 201
AjuI GAANNNNNNNTTGG 2 cut(s) 283, 315
AluBI AGCT 1 cut(s) 262
AluI AGCT 1 cut(s) 262
Alw26I GTCTC 1 cut(s) 196
AlwI GGATC 3 cut(s) 99, 215, 475
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 5 cut(s) 45, 199, 278, 425, 574
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 643
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 3 cut(s) 46, 278, 575
AsuC2I CCSGG 1 cut(s) 257
AsuHPI GGTGA 1 cut(s) 544
AvaI CYCGRG 1 cut(s) 335
BalI TGGCCA 1 cut(s) 427
BanI GGYRCC 1 cut(s) 37
BbvI GCAGC 1 cut(s) 41
BccI CCATC 4 cut(s) 114, 269, 365, 569
BceAI ACGGC 1 cut(s) 491
BciT130I CCWGG 1 cut(s) 203
BclI TGATCA 1 cut(s) 492
BcnI CCSGG 1 cut(s) 257
BcoDI GTCTC 1 cut(s) 196
BfaI CTAG 1 cut(s) 134
BfoI RGCGCY 1 cut(s) 41
BisI GCNGC 5 cut(s) 30, 33, 36, 328, 619
BlsI GCNGC 5 cut(s) 31, 34, 37, 329, 620
BmcAI AGTACT 1 cut(s) 83
Bme1390I CCNGG 2 cut(s) 203, 257
BmeT110I CYCGRG 1 cut(s) 335
BmgT120I GGNCC 3 cut(s) 46, 278, 575
BmiI GGNNCC 2 cut(s) 39, 130
BmrFI CCNGG 2 cut(s) 203, 257
BpuEI CTTGAG 1 cut(s) 284
BpuMI CCSGG 1 cut(s) 257
Bsa29I ATCGAT 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 202, 270
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bsc4I CCNNNNNNNGG 5 cut(s) 54, 139, 175, 276, 296
Bse1I ACTGG 4 cut(s) 48, 79, 449, 597
Bse3DI GCAATG 1 cut(s) 284
BseBI CCWGG 1 cut(s) 203
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 2 cut(s) 202, 270
BseGI GGATG 2 cut(s) 280, 415
BseLI CCNNNNNNNGG 5 cut(s) 54, 139, 175, 276, 296
BseMI GCAATG 1 cut(s) 284
BseNI ACTGG 4 cut(s) 48, 79, 449, 597
BseXI GCAGC 1 cut(s) 41
BshFI GGCC 5 cut(s) 47, 201, 280, 427, 576
BshNI GGYRCC 1 cut(s) 37
BshVI ATCGAT 1 cut(s) 585
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 1 cut(s) 257
BslI CCNNNNNNNGG 5 cut(s) 54, 139, 175, 276, 296
BsmAI GTCTC 1 cut(s) 196
BsmI GAATGC 2 cut(s) 74, 380
BsnI GGCC 5 cut(s) 47, 201, 280, 427, 576
BsoBI CYCGRG 1 cut(s) 335
Bsp143I GATC 4 cut(s) 91, 207, 480, 492
BspACI CCGC 6 cut(s) 24, 32, 35, 55, 328, 618
BspANI GGCC 5 cut(s) 47, 201, 280, 427, 576
BspDI ATCGAT 1 cut(s) 585
BspHI TCATGA 1 cut(s) 387
BspLI GGNNCC 2 cut(s) 39, 130
BspPI GGATC 3 cut(s) 99, 215, 475
BspT107I GGYRCC 1 cut(s) 37
BsrBI CCGCTC 1 cut(s) 618
BsrDI GCAATG 1 cut(s) 284
BsrI ACTGG 4 cut(s) 48, 79, 449, 597
BssECI CCNNGG 2 cut(s) 202, 270
BssMI GATC 4 cut(s) 91, 207, 480, 492
BssNI GRCGYC 1 cut(s) 38
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 112
Bst6I CTCTTC 1 cut(s) 521
BstACI GRCGYC 1 cut(s) 38
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 2 cut(s) 280, 415
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 4 cut(s) 94, 210, 483, 495
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 4 cut(s) 91, 207, 480, 492
BstMWI GCNNNNNNNGC 4 cut(s) 35, 44, 286, 433
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 2 cut(s) 201, 255
BstV1I GCAGC 1 cut(s) 41
BstX2I RGATCY 2 cut(s) 207, 480
BstYI RGATCY 2 cut(s) 207, 480
Bsu15I ATCGAT 1 cut(s) 585
BsuRI GGCC 5 cut(s) 47, 201, 280, 427, 576
BsuTUI ATCGAT 1 cut(s) 585
BtgI CCRYGG 1 cut(s) 270
BtsCI GGATG 2 cut(s) 280, 415
BtsIMutI CAGTG 3 cut(s) 41, 108, 590
CciI TCATGA 1 cut(s) 387
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 3 cut(s) 46, 278, 575
ClaI ATCGAT 1 cut(s) 585
Csp6I GTAC 3 cut(s) 82, 166, 413
CviAII CATG 3 cut(s) 170, 388, 490
CviQI GTAC 3 cut(s) 82, 166, 413
DinI GGCGCC 1 cut(s) 39
DpnI GATC 4 cut(s) 93, 209, 482, 494
DpnII GATC 4 cut(s) 91, 207, 480, 492
DraIII CACNNNGTG 1 cut(s) 112
EaeI YGGCCR 1 cut(s) 425
Eam1104I CTCTTC 1 cut(s) 521
EarI CTCTTC 1 cut(s) 521
EciI GGCGGA 2 cut(s) 39, 70
Eco32I GATATC 1 cut(s) 520
Eco88I CYCGRG 1 cut(s) 335
EcoO109I RGGNCCY 1 cut(s) 575
EcoRII CCWGG 1 cut(s) 201
EcoRV GATATC 1 cut(s) 520
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
FaeI CATG 3 cut(s) 173, 391, 493
FaiI YATR 8 cut(s) 146, 171, 186, 198, 389, 439, 491, 507
FatI CATG 3 cut(s) 169, 387, 489
FbaI TGATCA 1 cut(s) 492
Fnu4HI GCNGC 5 cut(s) 30, 33, 36, 328, 619
FokI GGATG 2 cut(s) 287, 422
Fsp4HI GCNGC 5 cut(s) 30, 33, 36, 328, 619
FspBI CTAG 1 cut(s) 134
GlaI GCGC 1 cut(s) 39
GluI GCNGC 5 cut(s) 30, 33, 36, 328, 619
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 5 cut(s) 47, 201, 280, 427, 576
HapII CCGG 1 cut(s) 257
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 3 cut(s) 173, 391, 493
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 2 cut(s) 99, 253
HindII GTYRAC 2 cut(s) 99, 253
HinfI GANTC 2 cut(s) 220, 266
HpaII CCGG 1 cut(s) 257
HphI GGTGA 1 cut(s) 544
Hpy166II GTNNAC 4 cut(s) 99, 166, 253, 539
Hpy188I TCNGA 3 cut(s) 304, 609, 636
Hpy188III TCNNGA 5 cut(s) 217, 226, 335, 388, 523
Hpy8I GTNNAC 4 cut(s) 99, 166, 253, 539
HpyAV CCTTC 3 cut(s) 241, 291, 341
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 1 cut(s) 380
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 44, 286, 433
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 3 cut(s) 173, 391, 493
HspAI GCGC 1 cut(s) 38
KasI GGCGCC 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 492
Kzo9I GATC 4 cut(s) 91, 207, 480, 492
LmnI GCTCC 4 cut(s) 26, 57, 259, 615
Lsp1109I GCAGC 1 cut(s) 41
MaeI CTAG 1 cut(s) 134
MaeII ACGT 1 cut(s) 213
MalI GATC 4 cut(s) 93, 209, 482, 494
MbiI CCGCTC 1 cut(s) 618
MboI GATC 4 cut(s) 91, 207, 480, 492
MboII GAAGA 2 cut(s) 229, 538
MflI RGATCY 2 cut(s) 207, 480
MlsI TGGCCA 1 cut(s) 427
MluCI AATT 2 cut(s) 9, 643
MluNI TGGCCA 1 cut(s) 427
Mly113I GGCGCC 1 cut(s) 38
MlyI GAGTC 1 cut(s) 275
MnlI CCTC 5 cut(s) 13, 114, 473, 522, 588
Mox20I TGGCCA 1 cut(s) 427
MscI TGGCCA 1 cut(s) 427
MseI TTAA 2 cut(s) 312, 342
Msp20I TGGCCA 1 cut(s) 427
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 1 cut(s) 257
MspR9I CCNGG 2 cut(s) 203, 257
Mva1269I GAATGC 2 cut(s) 74, 380
MvaI CCWGG 1 cut(s) 203
MwoI GCNNNNNNNGC 4 cut(s) 35, 44, 286, 433
NarI GGCGCC 1 cut(s) 38
NciI CCSGG 1 cut(s) 257
NdeII GATC 4 cut(s) 91, 207, 480, 492
NlaIII CATG 3 cut(s) 173, 391, 493
NlaIV GGNNCC 2 cut(s) 39, 130
PagI TCATGA 1 cut(s) 387
PctI GAATGC 2 cut(s) 74, 380
PfeI GAWTC 1 cut(s) 220
PflMI CCANNNNNTGG 1 cut(s) 296
PkrI GCNGC 5 cut(s) 31, 34, 37, 329, 620
PleI GAGTC 1 cut(s) 274
PluTI GGCGCC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 2 cut(s) 39, 130
PspPI GGNCC 3 cut(s) 46, 278, 575
PsuI RGATCY 2 cut(s) 207, 480
RsaI GTAC 3 cut(s) 83, 167, 414
RsaNI GTAC 3 cut(s) 82, 166, 413
SaqAI TTAA 2 cut(s) 312, 342
SatI GCNGC 5 cut(s) 30, 33, 36, 328, 619
Sau3AI GATC 4 cut(s) 91, 207, 480, 492
Sau96I GGNCC 3 cut(s) 46, 278, 575
ScaI AGTACT 1 cut(s) 83
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 2 cut(s) 203, 257
SetI ASST 5 cut(s) 216, 252, 264, 395, 418
SfoI GGCGCC 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 2 cut(s) 9, 643
SsiI CCGC 6 cut(s) 24, 32, 35, 55, 328, 618
SspDI GGCGCC 1 cut(s) 37
SspMI CTAG 1 cut(s) 134
StyD4I CCNGG 2 cut(s) 201, 255
TaaI ACNGT 1 cut(s) 112
TaiI ACGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 585
TaqII GACCGA 1 cut(s) 84
TasI AATT 2 cut(s) 9, 643
TatI WGTACW 1 cut(s) 81
TauI GCSGC 4 cut(s) 35, 38, 330, 621
TfiI GAWTC 1 cut(s) 220
Tru1I TTAA 2 cut(s) 312, 342
Tru9I TTAA 2 cut(s) 312, 342
TscAI CASTG 3 cut(s) 48, 115, 597
TseI GCWGC 1 cut(s) 29
TspDTI ATGAA 3 cut(s) 273, 373, 485
TspGWI ACGGA 1 cut(s) 259
TspRI CASTG 3 cut(s) 48, 115, 597
Van91I CCANNNNNTGG 1 cut(s) 296
XapI RAATTY 1 cut(s) 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 48
XspI CTAG 1 cut(s) 134
ZrmI AGTACT 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.