MD12G1149800.v1.1

Aldo-keto reductase family 4 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002704
Physical Location & Seq
Forward (+)
23012766 .. 23015244
2479 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1149800.v1.1.491

Sequence Viewer

Length: 948 bp
ATGGCGAAAGCAATTCGGTTCTTTGAGCTCAGCACTGGGGCCAAGATTCCGTCAGTCGGACTGGGAACTTGGCAGGCCGAGCCTGGCGTCGTCGCTGCCGCCGTCACCGCCGCCATTAAGATCGGATATAGGCATATTGATTGTGCTCAAGCTTATGGAAATGAAAAGGAGATTGGGTTTGCTCTGAAGAAGCTTTACGATGATGGTGTAGTGAAGCGGGAGGACTTGTGGATCACTTCAAAACTCTGGTGTAGTAATCATGCACCAGAAGATGTGCCAAAGGCACTAGATAGTACCCTGCAGGACTTGCAACTCGATTATCTCGATCTATATCTGATCCACTGGCCAGTCAAGATGAAGAGTGGTTCAGTTGGTTTTAAGCCTGAAAACCTTGACCAACCTGACATTCCCAGTACTTGGAAAGCACTGGAGGCAGCATATGATTCTGGCAAGGCCAGGGCTATTGGGGTTAGCAATTTTTCTACCAAAAAGCTAGGAGACCTGTTAGCGGTGGCGCGCATCCCTCCTGCTGTAAATCAAGTGGAAAGCCACCCTCAATGGCAGCAGCAGAAGCTACATGAATTCTGTAAATCCAAGGGAGTCCACTTAAGTGGATATTCACCACTGGGATCACCAGGCACAAGTAGCCTTAAGGGTGATGTCCTTAAGAATCCAATCCTATTGTCGGTAGCAGAGAAGCTGGGAAAGACTCCTGCTCAAGTGGCTATCCGGTGGGGATTACAGTCAGGCCACAGTGTTCTACCTAAGAGCACAAAGGAGGAAAGGATCAAGGAAAACTTTGATGTTTTTGACTGGTCTATACCTGAAGATTTGCTTGCAAACTTCGCTGAAGTAAAGCAGGAGAGGCTACTTAAAGGTACTGGATTCGTCAACGACACGCACGGTGCCTACAAGACCATCGAGGAACTCTGGGACGGCGAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00040 ko00561 ko00930 ko01100 ko01110 ko01120 ko01130 ko01220 map00010 map00040 map00561 map00930 map01100 map01110 map01120 map01130 map01220
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

316

Amino Acids

34.69

Weight (kDa)

6.46

Isoelectric Point (pI)

34.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 19 - 284 5.5e-53 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 905
AccB7I CCANNNNNTGG 1 cut(s) 417
AccII CGCG 1 cut(s) 517
AciI CCGC 5 cut(s) 99, 108, 111, 217, 509
AclWI GGATC 4 cut(s) 239, 331, 637, 794
AcoI YGGCCR 1 cut(s) 344
AcsI RAATTY 1 cut(s) 581
AcuI CTGAAG 3 cut(s) 206, 846, 870
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 3 cut(s) 295, 415, 880
AfiI CCNNNNNNNGG 4 cut(s) 56, 417, 508, 685
AflII CTTAAG 3 cut(s) 607, 650, 665
AgsI TTSAA 1 cut(s) 240
AjnI CCWGG 3 cut(s) 82, 455, 634
AjuI GAANNNNNNNTTGG 4 cut(s) 156, 188, 390, 422
AleI CACNNNNGTG 1 cut(s) 609
AluBI AGCT 6 cut(s) 28, 152, 193, 493, 574, 700
AluI AGCT 6 cut(s) 28, 152, 193, 493, 574, 700
Alw21I GWGCWC 3 cut(s) 30, 148, 773
Alw26I GTCTC 1 cut(s) 492
AlwI GGATC 4 cut(s) 239, 331, 637, 794
AoxI GGCC 5 cut(s) 39, 75, 344, 453, 748
ApeKI GCWGC 4 cut(s) 95, 434, 562, 565
ApoI RAATTY 1 cut(s) 581
AspLEI GCGC 2 cut(s) 517, 519
AspS9I GGNCC 1 cut(s) 39
AsuHPI GGTGA 4 cut(s) 97, 612, 624, 668
BalI TGGCCA 1 cut(s) 346
BanI GGYRCC 1 cut(s) 905
BanII GRGCYC 1 cut(s) 30
BarI GAAGNNNNNNTAC 2 cut(s) 179, 211
Bbv12I GWGCWC 3 cut(s) 30, 148, 773
BbvI GCAGC 4 cut(s) 82, 446, 574, 577
BccI CCATC 2 cut(s) 197, 926
BceAI ACGGC 1 cut(s) 86
BciT130I CCWGG 3 cut(s) 84, 457, 636
BcoDI GTCTC 1 cut(s) 492
BfaI CTAG 2 cut(s) 287, 494
BfmI CTRYAG 1 cut(s) 299
BfrI CTTAAG 3 cut(s) 607, 650, 665
BisI GCNGC 6 cut(s) 96, 99, 111, 435, 563, 566
BlpI GCTNAGC 1 cut(s) 29
BlsI GCNGC 6 cut(s) 97, 100, 112, 436, 564, 567
BmcAI AGTACT 1 cut(s) 415
Bme1390I CCNGG 3 cut(s) 84, 457, 636
BmgT120I GGNCC 1 cut(s) 39
BmiI GGNNCC 2 cut(s) 40, 907
BmrFI CCNGG 3 cut(s) 84, 457, 636
BmrI ACTGGG 4 cut(s) 45, 71, 405, 635
BmsI GCATC 1 cut(s) 528
BmuI ACTGGG 4 cut(s) 45, 71, 405, 635
BpmI CTGGAG 1 cut(s) 449
Bpu1102I GCTNAGC 1 cut(s) 29
BpuEI CTTGAG 2 cut(s) 132, 702
BsaBI GATNNNNATC 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 87
BsaI GGTCTC 1 cut(s) 492
BsaJI CCNNGG 2 cut(s) 456, 594
BsaWI WCCGGW 1 cut(s) 729
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 212, 242
Bsc4I CCNNNNNNNGG 4 cut(s) 56, 417, 508, 685
Bse1I ACTGG 9 cut(s) 40, 66, 347, 347, 411, 432, 630, 818, 886
Bse8I GATNNNNATC 1 cut(s) 330
BseBI CCWGG 3 cut(s) 84, 457, 636
BseDI CCNNGG 2 cut(s) 456, 594
BseGI GGATG 1 cut(s) 519
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 4 cut(s) 56, 417, 508, 685
BseMII CTCAG 1 cut(s) 43
BseNI ACTGG 9 cut(s) 40, 66, 347, 347, 411, 432, 630, 818, 886
BsePI GCGCGC 1 cut(s) 515
BseXI GCAGC 4 cut(s) 82, 446, 574, 577
BseYI CCCAGC 1 cut(s) 700
Bsh1236I CGCG 1 cut(s) 517
BshFI GGCC 5 cut(s) 41, 77, 346, 455, 750
BshNI GGYRCC 1 cut(s) 905
BsiHKAI GWGCWC 3 cut(s) 30, 148, 773
BsiSI CCGG 1 cut(s) 730
BslI CCNNNNNNNGG 4 cut(s) 56, 417, 508, 685
BsmAI GTCTC 1 cut(s) 492
BsnI GGCC 5 cut(s) 41, 77, 346, 455, 750
Bso31I GGTCTC 1 cut(s) 492
Bsp1286I GDGCHC 3 cut(s) 30, 148, 773
Bsp143I GATC 6 cut(s) 120, 231, 325, 336, 629, 786
Bsp1720I GCTNAGC 1 cut(s) 29
BspACI CCGC 5 cut(s) 99, 108, 111, 217, 509
BspANI GGCC 5 cut(s) 41, 77, 346, 455, 750
BspCNI CTCAG 1 cut(s) 42
BspFNI CGCG 1 cut(s) 517
BspLI GGNNCC 2 cut(s) 40, 907
BspMAI CTGCAG 1 cut(s) 303
BspPI GGATC 4 cut(s) 239, 331, 637, 794
BspT107I GGYRCC 1 cut(s) 905
BspTI CTTAAG 3 cut(s) 607, 650, 665
BspTNI GGTCTC 1 cut(s) 492
BsrI ACTGG 9 cut(s) 40, 66, 347, 347, 411, 432, 630, 818, 886
BssECI CCNNGG 2 cut(s) 456, 594
BssHII GCGCGC 1 cut(s) 515
BssMI GATC 6 cut(s) 120, 231, 325, 336, 629, 786
BssNI GRCGYC 1 cut(s) 87
BssT1I CCWWGG 1 cut(s) 594
Bst2UI CCWGG 3 cut(s) 84, 457, 636
Bst4CI ACNGT 3 cut(s) 744, 755, 905
Bst6I CTCTTC 1 cut(s) 353
BstACI GRCGYC 1 cut(s) 87
BstAFI CTTAAG 3 cut(s) 607, 650, 665
BstAPI GCANNNNNTGC 1 cut(s) 307
BstC8I GCNNGC 3 cut(s) 75, 517, 837
BstDEI CTNAG 2 cut(s) 29, 765
BstF5I GGATG 1 cut(s) 519
BstFNI CGCG 1 cut(s) 517
BstHHI GCGC 2 cut(s) 517, 519
BstKTI GATC 6 cut(s) 123, 234, 328, 339, 632, 789
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 6 cut(s) 120, 231, 325, 336, 629, 786
BstMWI GCNNNNNNNGC 9 cut(s) 79, 107, 307, 431, 571, 645, 722, 845, 865
BstNI CCWGG 3 cut(s) 84, 457, 636
BstSCI CCNGG 3 cut(s) 82, 455, 634
BstSFI CTRYAG 1 cut(s) 299
BstUI CGCG 1 cut(s) 517
BstV1I GCAGC 4 cut(s) 82, 446, 574, 577
BstXI CCANNNNNNTGG 1 cut(s) 611
BsuRI GGCC 5 cut(s) 41, 77, 346, 455, 750
BtsCI GGATG 1 cut(s) 519
BtsIMutI CAGTG 5 cut(s) 33, 340, 425, 623, 760
Cac8I GCNNGC 3 cut(s) 75, 517, 837
CfoI GCGC 2 cut(s) 517, 519
Cfr13I GGNCC 1 cut(s) 39
CseI GACGC 1 cut(s) 76
Csp6I GTAC 3 cut(s) 294, 414, 879
CviAII CATG 2 cut(s) 260, 578
CviQI GTAC 3 cut(s) 294, 414, 879
DdeI CTNAG 2 cut(s) 29, 765
DpnI GATC 6 cut(s) 122, 233, 327, 338, 631, 788
DpnII GATC 6 cut(s) 120, 231, 325, 336, 629, 786
EaeI YGGCCR 1 cut(s) 344
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 594
Eco24I GRGCYC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 492
Eco53kI GAGCTC 1 cut(s) 28
Eco57I CTGAAG 3 cut(s) 206, 846, 870
EcoICRI GAGCTC 1 cut(s) 28
EcoRI GAATTC 1 cut(s) 581
EcoRII CCWGG 3 cut(s) 82, 455, 634
EcoT14I CCWWGG 1 cut(s) 594
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 2 cut(s) 263, 581
FaiI YATR 9 cut(s) 129, 135, 156, 261, 331, 439, 441, 579, 821
FalI AAGNNNNNCTT 4 cut(s) 782, 814, 819, 851
FatI CATG 2 cut(s) 259, 577
FauI CCCGC 1 cut(s) 210
FauNDI CATATG 1 cut(s) 439
Fnu4HI GCNGC 6 cut(s) 96, 99, 111, 435, 563, 566
FokI GGATG 1 cut(s) 506
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 6 cut(s) 96, 99, 111, 435, 563, 566
FspBI CTAG 2 cut(s) 287, 494
GlaI GCGC 2 cut(s) 516, 518
GluI GCNGC 6 cut(s) 96, 99, 111, 435, 563, 566
GsaI CCCAGC 1 cut(s) 704
GsuI CTGGAG 1 cut(s) 449
HaeIII GGCC 5 cut(s) 41, 77, 346, 455, 750
HapII CCGG 1 cut(s) 730
HgaI GACGC 1 cut(s) 76
HhaI GCGC 2 cut(s) 517, 519
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 2 cut(s) 263, 581
Hin6I GCGC 2 cut(s) 515, 517
HinP1I GCGC 2 cut(s) 515, 517
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HindIII AAGCTT 2 cut(s) 150, 191
HinfI GANTC 6 cut(s) 46, 443, 600, 670, 709, 885
HpaII CCGG 1 cut(s) 730
HphI GGTGA 4 cut(s) 97, 612, 624, 668
Hpy166II GTNNAC 2 cut(s) 604, 892
Hpy188I TCNGA 4 cut(s) 59, 125, 186, 336
Hpy188III TCNNGA 2 cut(s) 323, 352
Hpy8I GTNNAC 2 cut(s) 604, 892
Hpy99I CGWCG 2 cut(s) 92, 95
HpyCH4III ACNGT 3 cut(s) 744, 755, 905
HpyCH4V TGCA 4 cut(s) 263, 301, 310, 839
HpyF10VI GCNNNNNNNGC 9 cut(s) 79, 107, 307, 431, 571, 645, 722, 845, 865
HpyF3I CTNAG 2 cut(s) 29, 765
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 2 cut(s) 263, 581
HspAI GCGC 2 cut(s) 515, 517
Kzo9I GATC 6 cut(s) 120, 231, 325, 336, 629, 786
Lsp1109I GCAGC 4 cut(s) 82, 446, 574, 577
LweI GCATC 1 cut(s) 528
MaeI CTAG 2 cut(s) 287, 494
MaeIII GTNAC 1 cut(s) 103
MalI GATC 6 cut(s) 122, 233, 327, 338, 631, 788
MboI GATC 6 cut(s) 120, 231, 325, 336, 629, 786
MboII GAAGA 4 cut(s) 199, 281, 370, 839
MhlI GDGCHC 3 cut(s) 30, 148, 773
MlsI TGGCCA 1 cut(s) 346
MluCI AATT 4 cut(s) 12, 475, 581, 941
MluNI TGGCCA 1 cut(s) 346
MlyI GAGTC 2 cut(s) 609, 703
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 7 cut(s) 214, 424, 534, 564, 772, 858, 916
Mox20I TGGCCA 1 cut(s) 346
MscI TGGCCA 1 cut(s) 346
MseI TTAA 6 cut(s) 117, 378, 608, 651, 666, 873
MslI CAYNNNNRTG 1 cut(s) 609
Msp20I TGGCCA 1 cut(s) 346
MspCI CTTAAG 3 cut(s) 607, 650, 665
MspI CCGG 1 cut(s) 730
MspR9I CCNGG 3 cut(s) 84, 457, 636
MvaI CCWGG 3 cut(s) 84, 457, 636
MvnI CGCG 1 cut(s) 517
MwoI GCNNNNNNNGC 9 cut(s) 79, 107, 307, 431, 571, 645, 722, 845, 865
NdeI CATATG 1 cut(s) 439
NdeII GATC 6 cut(s) 120, 231, 325, 336, 629, 786
NlaIII CATG 2 cut(s) 263, 581
NlaIV GGNNCC 2 cut(s) 40, 907
NmeAIII GCCGAG 1 cut(s) 103
NmuCI GTSAC 1 cut(s) 103
OliI CACNNNNGTG 1 cut(s) 609
PauI GCGCGC 1 cut(s) 515
PcsI WCGNNNNNNNCGW 2 cut(s) 99, 321
PfeI GAWTC 4 cut(s) 46, 443, 670, 885
PflMI CCANNNNNTGG 1 cut(s) 417
PkrI GCNGC 6 cut(s) 97, 100, 112, 436, 564, 567
PleI GAGTC 2 cut(s) 608, 703
PpsI GAGTC 2 cut(s) 608, 703
Psp124BI GAGCTC 1 cut(s) 30
Psp6I CCWGG 3 cut(s) 82, 455, 634
PspFI CCCAGC 1 cut(s) 700
PspGI CCWGG 3 cut(s) 82, 455, 634
PspN4I GGNNCC 2 cut(s) 40, 907
PspPI GGNCC 1 cut(s) 39
PstI CTGCAG 1 cut(s) 303
PteI GCGCGC 1 cut(s) 515
RsaI GTAC 3 cut(s) 295, 415, 880
RsaNI GTAC 3 cut(s) 294, 414, 879
RseI CAYNNNNRTG 1 cut(s) 609
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 6 cut(s) 117, 378, 608, 651, 666, 873
SatI GCNGC 6 cut(s) 96, 99, 111, 435, 563, 566
Sau3AI GATC 6 cut(s) 120, 231, 325, 336, 629, 786
Sau96I GGNCC 1 cut(s) 39
SbfI CCTGCAGG 1 cut(s) 303
ScaI AGTACT 1 cut(s) 415
SchI GAGTC 2 cut(s) 609, 703
ScrFI CCNGG 3 cut(s) 84, 457, 636
SdaI CCTGCAGG 1 cut(s) 303
SduI GDGCHC 3 cut(s) 30, 148, 773
SfaNI GCATC 1 cut(s) 528
SfcI CTRYAG 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 609
SmlI CTYRAG 5 cut(s) 147, 607, 650, 665, 717
SmoI CTYRAG 5 cut(s) 147, 607, 650, 665, 717
Sse8387I CCTGCAGG 1 cut(s) 303
Sse9I AATT 4 cut(s) 12, 475, 581, 941
SsiI CCGC 5 cut(s) 99, 108, 111, 217, 509
SspMI CTAG 2 cut(s) 287, 494
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 82, 455, 634
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 3 cut(s) 744, 755, 905
TaqI TCGA 3 cut(s) 315, 324, 921
TasI AATT 4 cut(s) 12, 475, 581, 941
TatI WGTACW 1 cut(s) 413
TauI GCSGC 2 cut(s) 101, 113
TfiI GAWTC 4 cut(s) 46, 443, 670, 885
Tru1I TTAA 6 cut(s) 117, 378, 608, 651, 666, 873
Tru9I TTAA 6 cut(s) 117, 378, 608, 651, 666, 873
TscAI CASTG 5 cut(s) 40, 347, 432, 630, 760
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 4 cut(s) 95, 434, 562, 565
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 3 cut(s) 177, 371, 594
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 5 cut(s) 40, 347, 432, 630, 760
Van91I CCANNNNNTGG 1 cut(s) 417
Vha464I CTTAAG 3 cut(s) 607, 650, 665
XapI RAATTY 1 cut(s) 581
XspI CTAG 2 cut(s) 287, 494
ZrmI AGTACT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.