Rh3DG141800

Aldo-keto reductase family 4 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002704
Physical Location & Seq
Reverse (-)
12118465 .. 12119571
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG141800.1

Sequence Viewer

Length: 588 bp
ATGCATTGGCCAGTCAGCATGAAAAAGGGGTCAGTTGGCTCTGATCCTGAAAAATTTACCACACCTAACATACCCAGTACATGGAAAGCAATGGAGGCACTGTATGATTCAGGCAAGGCCAGGGCAATTGGAGTCAGCAATTTCTCATCAAAGAAGCTTGGAGAACTGTTAGAGGTGGCACGAATACATCCAGCAGTTAATCAAGTTGAGTTGCACCCTCAATGGCAGCAAGCTAAGCTGCATTCTTTCTGTAAATCTAAGGGAATCCACTTAACTGGATATGCACCATTGGGAAGGCAAAAGGTGCTTGAACATTCTATTGTGAATATGGTGGCAGAAAAATTGGGCAAGACTCCTGCACAAGTAGTTCTTCGGTGGGGGATGCAAATGGGTCACAGTGTAGTGCCTAAAAGCACAAATCAGATGAGGATTCAGGAAAATTTTAATATTTTTGATTGGTCTATACCTGATAACTTGTTTGCCAAGTTTTCTGAAATTGAGCAGGAGAGGCTGCTTAAAGGTACTGCATTCGTCCACAAGACTCACGGTTTTTACAAGACCATTGAGGAACTCTGGGATGGAGAGTAG

Protein Analysis

195

Amino Acids

22.08

Weight (kDa)

9.28

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 2 - 166 2.4e-24 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 81
AclWI GGATC 1 cut(s) 38
AcoI YGGCCR 1 cut(s) 8
AcsI RAATTY 2 cut(s) 53, 439
AfaI GTAC 2 cut(s) 79, 523
AfiI CCNNNNNNNGG 1 cut(s) 81
AgsI TTSAA 1 cut(s) 311
AjnI CCWGG 1 cut(s) 119
AluBI AGCT 3 cut(s) 157, 233, 238
AluI AGCT 3 cut(s) 157, 233, 238
AlwI GGATC 1 cut(s) 38
AoxI GGCC 2 cut(s) 8, 117
ApeKI GCWGC 3 cut(s) 226, 238, 511
ApoI RAATTY 2 cut(s) 53, 439
BalI TGGCCA 1 cut(s) 10
BbvI GCAGC 3 cut(s) 225, 238, 498
BccI CCATC 1 cut(s) 572
BciT130I CCWGG 1 cut(s) 121
BisI GCNGC 3 cut(s) 227, 239, 512
BlpI GCTNAGC 1 cut(s) 234
BlsI GCNGC 3 cut(s) 228, 240, 513
Bme1390I CCNGG 1 cut(s) 121
BmrFI CCNGG 1 cut(s) 121
BmrI ACTGGG 1 cut(s) 69
BmsI GCATC 1 cut(s) 372
BmuI ACTGGG 1 cut(s) 69
Bpu1102I GCTNAGC 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 120
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 1 cut(s) 81
Bse1I ACTGG 3 cut(s) 11, 75, 280
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 3 cut(s) 187, 387, 583
BseLI CCNNNNNNNGG 1 cut(s) 81
BseMI GCAATG 1 cut(s) 96
BseNI ACTGG 3 cut(s) 11, 75, 280
BseXI GCAGC 3 cut(s) 225, 238, 498
BsgI GTGCAG 1 cut(s) 342
BshFI GGCC 2 cut(s) 10, 119
BslI CCNNNNNNNGG 1 cut(s) 81
BsmI GAATGC 2 cut(s) 241, 527
BsnI GGCC 2 cut(s) 10, 119
Bsp143I GATC 1 cut(s) 43
Bsp1720I GCTNAGC 1 cut(s) 234
BspANI GGCC 2 cut(s) 10, 119
BspPI GGATC 1 cut(s) 38
BsrDI GCAATG 1 cut(s) 96
BsrI ACTGG 3 cut(s) 11, 75, 280
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 1 cut(s) 43
Bst2UI CCWGG 1 cut(s) 121
Bst4CI ACNGT 4 cut(s) 102, 168, 398, 548
BstAPI GCANNNNNTGC 1 cut(s) 304
BstC8I GCNNGC 1 cut(s) 231
BstDEI CTNAG 2 cut(s) 234, 258
BstF5I GGATG 3 cut(s) 187, 387, 583
BstKTI GATC 1 cut(s) 46
BstMBI GATC 1 cut(s) 43
BstMWI GCNNNNNNNGC 4 cut(s) 95, 235, 304, 508
BstNI CCWGG 1 cut(s) 121
BstSCI CCNGG 1 cut(s) 119
BstV1I GCAGC 3 cut(s) 225, 238, 498
BstXI CCANNNNNNTGG 1 cut(s) 275
BsuRI GGCC 2 cut(s) 10, 119
BtsCI GGATG 3 cut(s) 187, 387, 583
BtsIMutI CAGTG 2 cut(s) 98, 403
Cac8I GCNNGC 1 cut(s) 231
Csp6I GTAC 2 cut(s) 78, 522
CviAII CATG 2 cut(s) 19, 81
CviJI RGCY 7 cut(s) 10, 39, 119, 157, 233, 238, 511
CviKI_1 RGCY 7 cut(s) 10, 39, 119, 157, 233, 238, 511
CviQI GTAC 2 cut(s) 78, 522
DdeI CTNAG 2 cut(s) 234, 258
DpnI GATC 1 cut(s) 45
DpnII GATC 1 cut(s) 43
EaeI YGGCCR 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 119
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 22, 84
FaiI YATR 7 cut(s) 20, 71, 82, 105, 282, 329, 464
FalI AAGNNNNNCTT 2 cut(s) 354, 386
FatI CATG 2 cut(s) 18, 80
Fnu4HI GCNGC 3 cut(s) 227, 239, 512
FokI GGATG 2 cut(s) 174, 394
Fsp4HI GCNGC 3 cut(s) 227, 239, 512
GluI GCNGC 3 cut(s) 227, 239, 512
HaeIII GGCC 2 cut(s) 10, 119
Hin1II CATG 2 cut(s) 22, 84
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 6 cut(s) 107, 132, 264, 352, 430, 541
Hpy166II GTNNAC 1 cut(s) 535
Hpy188I TCNGA 3 cut(s) 43, 423, 493
Hpy188III TCNNGA 2 cut(s) 47, 434
Hpy8I GTNNAC 1 cut(s) 535
HpyAV CCTTC 1 cut(s) 288
HpyCH4III ACNGT 4 cut(s) 102, 168, 398, 548
HpyCH4V TGCA 7 cut(s) 4, 214, 241, 284, 359, 385, 527
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 235, 304, 508
HpyF3I CTNAG 2 cut(s) 234, 258
Hsp92II CATG 2 cut(s) 22, 84
Kzo9I GATC 1 cut(s) 43
Lsp1109I GCAGC 3 cut(s) 225, 238, 498
LweI GCATC 1 cut(s) 372
MaeIII GTNAC 1 cut(s) 392
MalI GATC 1 cut(s) 45
MboI GATC 1 cut(s) 43
MboII GAAGA 1 cut(s) 362
MfeI CAATTG 1 cut(s) 126
MlsI TGGCCA 1 cut(s) 10
MluCI AATT 6 cut(s) 53, 126, 139, 341, 439, 495
MluNI TGGCCA 1 cut(s) 10
MlyI GAGTC 3 cut(s) 141, 346, 535
MnlI CCTC 6 cut(s) 88, 166, 228, 420, 501, 559
Mox20I TGGCCA 1 cut(s) 10
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 10
MseI TTAA 4 cut(s) 198, 272, 444, 516
Msp20I TGGCCA 1 cut(s) 10
MspR9I CCNGG 1 cut(s) 121
MunI CAATTG 1 cut(s) 126
Mva1269I GAATGC 2 cut(s) 241, 527
MvaI CCWGG 1 cut(s) 121
MwoI GCNNNNNNNGC 4 cut(s) 95, 235, 304, 508
NdeII GATC 1 cut(s) 43
NlaIII CATG 2 cut(s) 22, 84
NmuCI GTSAC 1 cut(s) 392
NsiI ATGCAT 1 cut(s) 6
PctI GAATGC 2 cut(s) 241, 527
PfeI GAWTC 3 cut(s) 107, 264, 430
PflMI CCANNNNNTGG 1 cut(s) 81
PkrI GCNGC 3 cut(s) 228, 240, 513
PleI GAGTC 3 cut(s) 140, 346, 535
PpsI GAGTC 3 cut(s) 140, 346, 535
Psp6I CCWGG 1 cut(s) 119
PspGI CCWGG 1 cut(s) 119
RsaI GTAC 2 cut(s) 79, 523
RsaNI GTAC 2 cut(s) 78, 522
SaqAI TTAA 4 cut(s) 198, 272, 444, 516
SatI GCNGC 3 cut(s) 227, 239, 512
Sau3AI GATC 1 cut(s) 43
SchI GAGTC 3 cut(s) 141, 346, 535
ScrFI CCNGG 1 cut(s) 121
SetI ASST 8 cut(s) 67, 159, 177, 235, 240, 306, 469, 523
SfaNI GCATC 1 cut(s) 372
Sse9I AATT 6 cut(s) 53, 126, 139, 341, 439, 495
SspI AATATT 1 cut(s) 448
StyD4I CCNGG 1 cut(s) 119
TaaI ACNGT 4 cut(s) 102, 168, 398, 548
TasI AATT 6 cut(s) 53, 126, 139, 341, 439, 495
TatI WGTACW 1 cut(s) 77
TfiI GAWTC 3 cut(s) 107, 264, 430
Tru1I TTAA 4 cut(s) 198, 272, 444, 516
Tru9I TTAA 4 cut(s) 198, 272, 444, 516
TscAI CASTG 2 cut(s) 105, 403
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 3 cut(s) 226, 238, 511
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 1 cut(s) 35
TspRI CASTG 2 cut(s) 105, 403
Van91I CCANNNNNTGG 1 cut(s) 81
XapI RAATTY 2 cut(s) 53, 439
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.