Rw3G011520

Aldo-keto reductase family 4 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002704
Physical Location & Seq
Reverse (-)
11199246 .. 11201169
1924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G011520.1

Sequence Viewer

Length: 948 bp
ATGGCGAATGCGATTCGATTCTTTGAGCTGAACACCGGAGCGAAGATTCCGGCCGTCGGTTTGGGGACGTGGCAGGCCGAGCCCGGCGTCGTTGGCGATGCTGTCACCGCCGCCATCAAGGTTGGGTATAGGCATATTGATTGTGCTCAAATTTATGGAAACGAAAAAGAGATTGGTTGTGCTCTGAAGAAGCTATATGATGATGGTGTAGTGAAGCGTGAGGACTTGTGGATCACTTCAAAACTCTGGTGTAGTAATCATGCACCAGAAGATGTGCCAAAGGCACTAGATGGCACCCTGCAGGACTTGCAAAGTGACTATCTCGATCTGTACCTGATTCACTGGCCAGTCAGCATGAAAAAGGGCTCAACAGGCTTTAAACCTGAAAACTTTTCTCAACCCGACCTACCAAGTACTTGGAAAGCAATGGAAGCACTGTATGATTCTGGCAAGGCCAAGGCTATAGGAGTTAGCAATTTTTCCACAAAAAAACTTGGAGATCTGTTATCGGTGGCACGCATACCTCCTGCTGTTAACCAAGTGGAATTGCACCCCCAATGGCAGCAGCCAAAGCTAAAAGCATTCTGCCAATCTAAGGGAGTCCACTTAACTGGGTATTCGCCATTGGGATCCCCAGGTACAAGTAGTATTAAGGGTGATGTCCTTAAGAATCCGGTTCTTGCATTGGTAGCAGAGAAGCTGGGCAAGACTCCTGCACAAGTGGCTCTCCGTTGGGGGCTACAGTCGGGTCACAGTGTGCTGCCTAAGAGCACAAAGGAGGCGAGGATCAAGGAGAATTTTGATGTTTTTGATTGGTCCATACCTGAGGACTTGGTTTCGAAGTTTTCTGATATAAAGCAGGAGAGGCTGCTTAAAGGCACTGGATTCGTCAATGAGACTCACGGTTTCTACAAGAACATTGAGGAACTTTGGGATGGAGAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00040 ko00561 ko00930 ko01100 ko01110 ko01120 ko01130 ko01220 map00010 map00040 map00561 map00930 map01100 map01110 map01120 map01130 map01220
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

315

Amino Acids

34.69

Weight (kDa)

7.05

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 19 - 283 1.6e-51 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 293
AciI CCGC 2 cut(s) 108, 111
AclWI GGATC 4 cut(s) 239, 624, 637, 794
AcoI YGGCCR 2 cut(s) 51, 344
AcsI RAATTY 2 cut(s) 150, 796
AcuI CTGAAG 1 cut(s) 206
AcyI GRCGYC 1 cut(s) 87
AdeI CACNNNGTG 1 cut(s) 757
AfaI GTAC 3 cut(s) 332, 415, 640
AfiI CCNNNNNNNGG 2 cut(s) 56, 595
AflII CTTAAG 1 cut(s) 665
AgsI TTSAA 1 cut(s) 240
AjiI CACGTC 1 cut(s) 69
AjnI CCWGG 1 cut(s) 634
AjuI GAANNNNNNNTTGG 2 cut(s) 156, 188
AluBI AGCT 4 cut(s) 28, 193, 574, 700
AluI AGCT 4 cut(s) 28, 193, 574, 700
Alw21I GWGCWC 3 cut(s) 148, 184, 773
Alw26I GTCTC 1 cut(s) 890
AlwI GGATC 4 cut(s) 239, 624, 637, 794
AoxI GGCC 4 cut(s) 51, 75, 344, 453
ApeKI GCWGC 4 cut(s) 562, 565, 760, 868
ApoI RAATTY 2 cut(s) 150, 796
AspS9I GGNCC 1 cut(s) 816
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 2 cut(s) 97, 668
AsuII TTCGAA 1 cut(s) 839
AvaII GGWCC 1 cut(s) 816
AxyI CCTNAGG 1 cut(s) 825
BalI TGGCCA 1 cut(s) 346
BamHI GGATCC 1 cut(s) 629
BanI GGYRCC 1 cut(s) 293
BanII GRGCYC 2 cut(s) 84, 368
Bbv12I GWGCWC 3 cut(s) 148, 184, 773
BbvI GCAGC 4 cut(s) 574, 577, 747, 855
BccI CCATC 4 cut(s) 122, 197, 284, 929
BceAI ACGGC 1 cut(s) 38
BcgI CGANNNNNNTGC 2 cut(s) 868, 902
BciT130I CCWGG 1 cut(s) 636
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 1 cut(s) 890
BfaI CTAG 1 cut(s) 287
BfmI CTRYAG 3 cut(s) 299, 462, 740
BfrI CTTAAG 1 cut(s) 665
BglII AGATCT 1 cut(s) 499
BisI GCNGC 5 cut(s) 111, 563, 566, 761, 869
BlsI GCNGC 5 cut(s) 112, 564, 567, 762, 870
BmcAI AGTACT 1 cut(s) 415
Bme1390I CCNGG 2 cut(s) 84, 636
Bme18I GGWCC 1 cut(s) 816
BmgBI CACGTC 1 cut(s) 69
BmgT120I GGNCC 1 cut(s) 816
BmiI GGNNCC 2 cut(s) 295, 631
BmrFI CCNGG 2 cut(s) 84, 636
BmrI ACTGGG 1 cut(s) 621
BmsI GCATC 1 cut(s) 88
BmuI ACTGGG 1 cut(s) 621
Bpu14I TTCGAA 1 cut(s) 839
BpuMI CCSGG 1 cut(s) 84
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 2 cut(s) 456, 634
BsaWI WCCGGW 2 cut(s) 35, 673
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 595
Bse1I ACTGG 4 cut(s) 347, 347, 616, 886
Bse21I CCTNAGG 1 cut(s) 825
Bse3DI GCAATG 1 cut(s) 432
BseBI CCWGG 1 cut(s) 636
BseDI CCNNGG 2 cut(s) 456, 634
BseGI GGATG 1 cut(s) 940
BseLI CCNNNNNNNGG 2 cut(s) 56, 595
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 1 cut(s) 816
BseNI ACTGG 4 cut(s) 347, 347, 616, 886
BseX3I CGGCCG 1 cut(s) 51
BseXI GCAGC 4 cut(s) 574, 577, 747, 855
BseYI CCCAGC 1 cut(s) 700
BsgI GTGCAG 1 cut(s) 699
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 4 cut(s) 53, 77, 346, 455
BshNI GGYRCC 1 cut(s) 293
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 3 cut(s) 148, 184, 773
BsiSI CCGG 4 cut(s) 36, 50, 84, 674
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 2 cut(s) 56, 595
BsmAI GTCTC 1 cut(s) 890
BsmFI GGGAC 1 cut(s) 79
BsmI GAATGC 2 cut(s) 13, 581
BsnI GGCC 4 cut(s) 53, 77, 346, 455
Bsp119I TTCGAA 1 cut(s) 839
Bsp1286I GDGCHC 5 cut(s) 84, 148, 184, 368, 773
Bsp143I GATC 5 cut(s) 231, 325, 499, 629, 786
BspACI CCGC 2 cut(s) 108, 111
BspANI GGCC 4 cut(s) 53, 77, 346, 455
BspCNI CTCAG 1 cut(s) 817
BspLI GGNNCC 2 cut(s) 295, 631
BspMAI CTGCAG 1 cut(s) 303
BspPI GGATC 4 cut(s) 239, 624, 637, 794
BspT104I TTCGAA 1 cut(s) 839
BspT107I GGYRCC 1 cut(s) 293
BspTI CTTAAG 1 cut(s) 665
BsrDI GCAATG 1 cut(s) 432
BsrI ACTGG 4 cut(s) 347, 347, 616, 886
BssECI CCNNGG 2 cut(s) 456, 634
BssMI GATC 5 cut(s) 231, 325, 499, 629, 786
BssNI GRCGYC 1 cut(s) 87
BssT1I CCWWGG 1 cut(s) 456
Bst2UI CCWGG 1 cut(s) 636
Bst4CI ACNGT 4 cut(s) 438, 744, 755, 905
BstACI GRCGYC 1 cut(s) 87
BstAFI CTTAAG 1 cut(s) 665
BstAPI GCANNNNNTGC 1 cut(s) 307
BstBI TTCGAA 1 cut(s) 839
BstC8I GCNNGC 2 cut(s) 75, 517
BstDEI CTNAG 3 cut(s) 594, 765, 825
BstF5I GGATG 1 cut(s) 940
BstKTI GATC 5 cut(s) 234, 328, 502, 632, 789
BstMAI GTCTC 1 cut(s) 890
BstMBI GATC 5 cut(s) 231, 325, 499, 629, 786
BstMCI CGRYCG 1 cut(s) 54
BstNI CCWGG 1 cut(s) 636
BstSCI CCNGG 2 cut(s) 82, 634
BstSFI CTRYAG 3 cut(s) 299, 462, 740
BstV1I GCAGC 4 cut(s) 574, 577, 747, 855
BstX2I RGATCY 2 cut(s) 499, 629
BstXI CCANNNNNNTGG 2 cut(s) 417, 611
BstYI RGATCY 2 cut(s) 499, 629
BstZI CGGCCG 1 cut(s) 51
Bsu36I CCTNAGG 1 cut(s) 825
BsuRI GGCC 4 cut(s) 53, 77, 346, 455
BtgZI GCGATG 1 cut(s) 111
BtrI CACGTC 1 cut(s) 69
BtsCI GGATG 1 cut(s) 940
BtsIMutI CAGTG 4 cut(s) 340, 434, 760, 879
Cac8I GCNNGC 2 cut(s) 75, 517
Cfr13I GGNCC 1 cut(s) 816
CseI GACGC 1 cut(s) 76
Csp6I GTAC 3 cut(s) 331, 414, 639
CviAII CATG 2 cut(s) 260, 355
CviQI GTAC 3 cut(s) 331, 414, 639
DdeI CTNAG 3 cut(s) 594, 765, 825
DpnI GATC 5 cut(s) 233, 327, 501, 631, 788
DpnII GATC 5 cut(s) 231, 325, 499, 629, 786
DraI TTTAAA 1 cut(s) 379
DraIII CACNNNGTG 1 cut(s) 757
EaeI YGGCCR 2 cut(s) 51, 344
EagI CGGCCG 1 cut(s) 51
EclXI CGGCCG 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 456
Eco24I GRGCYC 2 cut(s) 84, 368
Eco47I GGWCC 1 cut(s) 816
Eco52I CGGCCG 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 206
Eco81I CCTNAGG 1 cut(s) 825
EcoRII CCWGG 1 cut(s) 634
EcoT14I CCWWGG 1 cut(s) 456
EcoT38I GRGCYC 2 cut(s) 84, 368
ErhI CCWWGG 1 cut(s) 456
FaeI CATG 2 cut(s) 263, 358
FaqI GGGAC 1 cut(s) 79
FatI CATG 2 cut(s) 259, 354
Fnu4HI GCNGC 5 cut(s) 111, 563, 566, 761, 869
FriOI GRGCYC 2 cut(s) 84, 368
Fsp4HI GCNGC 5 cut(s) 111, 563, 566, 761, 869
FspBI CTAG 1 cut(s) 287
GluI GCNGC 5 cut(s) 111, 563, 566, 761, 869
GsaI CCCAGC 1 cut(s) 704
HaeIII GGCC 4 cut(s) 53, 77, 346, 455
HapII CCGG 4 cut(s) 36, 50, 84, 674
HgaI GACGC 1 cut(s) 76
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 2 cut(s) 263, 358
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HpaI GTTAAC 1 cut(s) 535
HpaII CCGG 4 cut(s) 36, 50, 84, 674
HphI GGTGA 2 cut(s) 97, 668
Hpy166II GTNNAC 2 cut(s) 535, 604
Hpy188I TCNGA 2 cut(s) 186, 850
Hpy188III TCNNGA 1 cut(s) 323
Hpy8I GTNNAC 2 cut(s) 535, 604
Hpy99I CGWCG 2 cut(s) 59, 92
HpyCH4III ACNGT 4 cut(s) 438, 744, 755, 905
HpyCH4IV ACGT 1 cut(s) 68
HpyCH4V TGCA 6 cut(s) 263, 301, 310, 550, 683, 716
HpyF3I CTNAG 3 cut(s) 594, 765, 825
HpySE526I ACGT 1 cut(s) 68
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 2 cut(s) 263, 358
KspAI GTTAAC 1 cut(s) 535
Kzo9I GATC 5 cut(s) 231, 325, 499, 629, 786
LmnI GCTCC 1 cut(s) 38
Lsp1109I GCAGC 4 cut(s) 574, 577, 747, 855
LweI GCATC 1 cut(s) 88
MaeI CTAG 1 cut(s) 287
MaeII ACGT 1 cut(s) 68
MaeIII GTNAC 3 cut(s) 103, 314, 749
MalI GATC 5 cut(s) 233, 327, 501, 631, 788
MboI GATC 5 cut(s) 231, 325, 499, 629, 786
MboII GAAGA 3 cut(s) 55, 199, 281
MflI RGATCY 2 cut(s) 499, 629
MhlI GDGCHC 5 cut(s) 84, 148, 184, 368, 773
MlsI TGGCCA 1 cut(s) 346
MluCI AATT 5 cut(s) 150, 475, 545, 796, 941
MluNI TGGCCA 1 cut(s) 346
MlyI GAGTC 3 cut(s) 609, 703, 892
MnlI CCTC 7 cut(s) 214, 534, 772, 777, 820, 858, 916
Mox20I TGGCCA 1 cut(s) 346
MscI TGGCCA 1 cut(s) 346
MseI TTAA 6 cut(s) 378, 534, 608, 651, 666, 873
Msp20I TGGCCA 1 cut(s) 346
MspCI CTTAAG 1 cut(s) 665
MspI CCGG 4 cut(s) 36, 50, 84, 674
MspR9I CCNGG 2 cut(s) 84, 636
Mva1269I GAATGC 2 cut(s) 13, 581
MvaI CCWGG 1 cut(s) 636
NciI CCSGG 1 cut(s) 84
NdeII GATC 5 cut(s) 231, 325, 499, 629, 786
NlaIII CATG 2 cut(s) 263, 358
NlaIV GGNNCC 2 cut(s) 295, 631
NmeAIII GCCGAG 1 cut(s) 103
NmuCI GTSAC 3 cut(s) 103, 314, 749
NspV TTCGAA 1 cut(s) 839
PctI GAATGC 2 cut(s) 13, 581
PfeI GAWTC 7 cut(s) 13, 18, 46, 337, 443, 670, 885
PkrI GCNGC 5 cut(s) 112, 564, 567, 762, 870
PleI GAGTC 3 cut(s) 608, 703, 892
PpsI GAGTC 3 cut(s) 608, 703, 892
Psp6I CCWGG 1 cut(s) 634
PspFI CCCAGC 1 cut(s) 700
PspGI CCWGG 1 cut(s) 634
PspN4I GGNNCC 2 cut(s) 295, 631
PspPI GGNCC 1 cut(s) 816
PstI CTGCAG 1 cut(s) 303
PsuI RGATCY 2 cut(s) 499, 629
RsaI GTAC 3 cut(s) 332, 415, 640
RsaNI GTAC 3 cut(s) 331, 414, 639
SaqAI TTAA 6 cut(s) 378, 534, 608, 651, 666, 873
SatI GCNGC 5 cut(s) 111, 563, 566, 761, 869
Sau3AI GATC 5 cut(s) 231, 325, 499, 629, 786
Sau96I GGNCC 1 cut(s) 816
SbfI CCTGCAGG 1 cut(s) 303
ScaI AGTACT 1 cut(s) 415
SchI GAGTC 3 cut(s) 609, 703, 892
ScrFI CCNGG 2 cut(s) 84, 636
SdaI CCTGCAGG 1 cut(s) 303
SduI GDGCHC 5 cut(s) 84, 148, 184, 368, 773
SfaNI GCATC 1 cut(s) 88
SfcI CTRYAG 3 cut(s) 299, 462, 740
SfuI TTCGAA 1 cut(s) 839
SinI GGWCC 1 cut(s) 816
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
Sse8387I CCTGCAGG 1 cut(s) 303
Sse9I AATT 5 cut(s) 150, 475, 545, 796, 941
SsiI CCGC 2 cut(s) 108, 111
SspMI CTAG 1 cut(s) 287
StyD4I CCNGG 2 cut(s) 82, 634
StyI CCWWGG 1 cut(s) 456
TaaI ACNGT 4 cut(s) 438, 744, 755, 905
TaiI ACGT 1 cut(s) 71
TaqI TCGA 3 cut(s) 16, 324, 839
TasI AATT 5 cut(s) 150, 475, 545, 796, 941
TatI WGTACW 1 cut(s) 413
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 7 cut(s) 13, 18, 46, 337, 443, 670, 885
Tru1I TTAA 6 cut(s) 378, 534, 608, 651, 666, 873
Tru9I TTAA 6 cut(s) 378, 534, 608, 651, 666, 873
TscAI CASTG 4 cut(s) 347, 441, 760, 886
TseFI GTSAC 3 cut(s) 103, 314, 749
TseI GCWGC 4 cut(s) 562, 565, 760, 868
Tsp45I GTSAC 3 cut(s) 103, 314, 749
TspDTI ATGAA 1 cut(s) 371
TspGWI ACGGA 1 cut(s) 719
TspRI CASTG 4 cut(s) 347, 441, 760, 886
Vha464I CTTAAG 1 cut(s) 665
VpaK11BI GGWCC 1 cut(s) 816
XapI RAATTY 2 cut(s) 150, 796
XspI CTAG 1 cut(s) 287
ZrmI AGTACT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.