pycom12g14360

Aldo-keto reductase family 4 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002704
Physical Location & Seq
Forward (+)
16934707 .. 16936810
2104 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g14360.7

Sequence Viewer

Length: 948 bp
ATGGCGAAAGCAATTCGGTTCTTTGAGCTCAGCACTGGGGCCAAGATTCCGTCAGTCGGACTGGGAACCTGGCAGGCCGAGCCTGGCGTCGTCGCTGCTGCCGTCACCGCAGCCATTAAGATCGGATATAGGCATATTGATTGTGCTCAAGCTTATGGAAATGAAAAGGAGATTGGGTTTGCTCTGAAGAAGCTTTACGATGATGGTGTAGTGAAGCGGGAGGACTTGTGGATCACTTCAAAACTCTGGTGTAGCAATCATGCACCAGAAGATGTGCCAAAGGCACTAGATAGTACCCTGCAGGACTTGCAACTCGATTATCTCGATCTGTATCTGATCCACTGGCCTGTCAAGATGAAGAGTGGTTCAGTTGGTTTTAAGCCTGAAAACCTGGACCAACCTGACATTCCCAGTACTTGGAAAGCACTGGAGGCAGCATATGATTCTGGCAAGGCCAGGGCTATTGGGGTTAGCAATTTTTCTACCAAAAAGCTAGGAGACCTGTTAGCAGTGGCGCGCATCCCTCCTGCTGTTAATCAAGTGGAAAGCCACCCTCAATGGCAGCAGCAGAAGCTGCATGAATTCTGTAAATCCAAGGGAGTCCACTTAAGTGGATATTCACCACTGGGATCCCCAGGCACAAGTAGCATTAAGGGTGATGTCCTTAAGAATCCAATCCTATTGTCGGTAGCAGAGAAGCTGGCAAAGACTCCTGCTCAAGTGGCTATCCGGTGGGGATTACAGTTGGGCCACAGTGTTCTGCCTAAGAGCACAAAGGGGGAAAGGATCAAGGAAAACTTTGATGTTTTTGACTGGTCTATACCTGAAGACTTGCTTGCAAACTTCGCTGAAGTAAAGCAGGAGAGGCTACTTAAAGGTACTGGATTCGTCAACGACACGCACGGTGCCTACAAGACCATCGAGGAACTCTGGGACGGCGAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00040 ko00561 ko00930 ko01100 ko01110 ko01120 ko01130 ko01220 map00010 map00040 map00561 map00930 map01100 map01110 map01120 map01130 map01220
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

316

Amino Acids

34.66

Weight (kDa)

6.72

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 905
AccB7I CCANNNNNTGG 1 cut(s) 417
AccII CGCG 1 cut(s) 517
AciI CCGC 2 cut(s) 108, 217
AclWI GGATC 5 cut(s) 239, 331, 624, 637, 794
AcsI RAATTY 1 cut(s) 581
AcuI CTGAAG 3 cut(s) 206, 846, 870
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 3 cut(s) 295, 415, 880
AfiI CCNNNNNNNGG 3 cut(s) 56, 417, 685
AflII CTTAAG 2 cut(s) 607, 665
AgsI TTSAA 1 cut(s) 240
AjnI CCWGG 5 cut(s) 68, 82, 390, 455, 634
AjuI GAANNNNNNNTTGG 4 cut(s) 156, 188, 390, 422
AleI CACNNNNGTG 1 cut(s) 609
AluBI AGCT 6 cut(s) 28, 152, 193, 493, 574, 700
AluI AGCT 6 cut(s) 28, 152, 193, 493, 574, 700
Alw21I GWGCWC 3 cut(s) 30, 148, 773
Alw26I GTCTC 1 cut(s) 492
AlwI GGATC 5 cut(s) 239, 331, 624, 637, 794
AlwNI CAGNNNCTG 1 cut(s) 574
AoxI GGCC 5 cut(s) 39, 75, 344, 453, 748
ApeKI GCWGC 7 cut(s) 95, 98, 110, 434, 562, 565, 574
ApoI RAATTY 1 cut(s) 581
AspLEI GCGC 2 cut(s) 517, 519
AspS9I GGNCC 3 cut(s) 39, 394, 748
AsuHPI GGTGA 3 cut(s) 97, 612, 668
AvaII GGWCC 1 cut(s) 394
BamHI GGATCC 1 cut(s) 629
BanI GGYRCC 1 cut(s) 905
BanII GRGCYC 1 cut(s) 30
BarI GAAGNNNNNNTAC 2 cut(s) 179, 211
BbsI GAAGAC 1 cut(s) 834
Bbv12I GWGCWC 3 cut(s) 30, 148, 773
BbvI GCAGC 7 cut(s) 82, 85, 122, 446, 561, 574, 577
BccI CCATC 2 cut(s) 197, 926
BceAI ACGGC 1 cut(s) 86
BciT130I CCWGG 5 cut(s) 70, 84, 392, 457, 636
BcoDI GTCTC 1 cut(s) 492
BfaI CTAG 2 cut(s) 287, 494
BfmI CTRYAG 1 cut(s) 299
BfrI CTTAAG 2 cut(s) 607, 665
BisI GCNGC 7 cut(s) 96, 99, 111, 435, 563, 566, 575
BlpI GCTNAGC 1 cut(s) 29
BlsI GCNGC 7 cut(s) 97, 100, 112, 436, 564, 567, 576
BmcAI AGTACT 1 cut(s) 415
Bme1390I CCNGG 5 cut(s) 70, 84, 392, 457, 636
Bme18I GGWCC 1 cut(s) 394
BmgT120I GGNCC 3 cut(s) 39, 394, 748
BmiI GGNNCC 4 cut(s) 40, 67, 631, 907
BmrFI CCNGG 5 cut(s) 70, 84, 392, 457, 636
BmrI ACTGGG 4 cut(s) 45, 71, 405, 635
BmsI GCATC 1 cut(s) 528
BmuI ACTGGG 4 cut(s) 45, 71, 405, 635
BpiI GAAGAC 1 cut(s) 834
BpmI CTGGAG 1 cut(s) 449
Bpu1102I GCTNAGC 1 cut(s) 29
BpuEI CTTGAG 2 cut(s) 132, 702
BsaBI GATNNNNATC 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 87
BsaI GGTCTC 1 cut(s) 492
BsaJI CCNNGG 3 cut(s) 456, 594, 634
BsaWI WCCGGW 1 cut(s) 729
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 212, 242
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 417, 685
Bse1I ACTGG 8 cut(s) 40, 66, 347, 411, 432, 630, 818, 886
Bse8I GATNNNNATC 1 cut(s) 330
BseBI CCWGG 5 cut(s) 70, 84, 392, 457, 636
BseDI CCNNGG 3 cut(s) 456, 594, 634
BseGI GGATG 1 cut(s) 519
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 3 cut(s) 56, 417, 685
BseMII CTCAG 1 cut(s) 43
BseNI ACTGG 8 cut(s) 40, 66, 347, 411, 432, 630, 818, 886
BsePI GCGCGC 1 cut(s) 515
BseXI GCAGC 7 cut(s) 82, 85, 122, 446, 561, 574, 577
Bsh1236I CGCG 1 cut(s) 517
BshFI GGCC 5 cut(s) 41, 77, 346, 455, 750
BshNI GGYRCC 1 cut(s) 905
BsiHKAI GWGCWC 3 cut(s) 30, 148, 773
BsiSI CCGG 1 cut(s) 730
BslI CCNNNNNNNGG 3 cut(s) 56, 417, 685
BsmAI GTCTC 1 cut(s) 492
BsnI GGCC 5 cut(s) 41, 77, 346, 455, 750
Bso31I GGTCTC 1 cut(s) 492
Bsp1286I GDGCHC 3 cut(s) 30, 148, 773
Bsp143I GATC 6 cut(s) 120, 231, 325, 336, 629, 786
Bsp1720I GCTNAGC 1 cut(s) 29
BspACI CCGC 2 cut(s) 108, 217
BspANI GGCC 5 cut(s) 41, 77, 346, 455, 750
BspCNI CTCAG 1 cut(s) 42
BspFNI CGCG 1 cut(s) 517
BspLI GGNNCC 4 cut(s) 40, 67, 631, 907
BspMAI CTGCAG 1 cut(s) 303
BspPI GGATC 5 cut(s) 239, 331, 624, 637, 794
BspT107I GGYRCC 1 cut(s) 905
BspTI CTTAAG 2 cut(s) 607, 665
BspTNI GGTCTC 1 cut(s) 492
BsrI ACTGG 8 cut(s) 40, 66, 347, 411, 432, 630, 818, 886
BssECI CCNNGG 3 cut(s) 456, 594, 634
BssHII GCGCGC 1 cut(s) 515
BssMI GATC 6 cut(s) 120, 231, 325, 336, 629, 786
BssNI GRCGYC 1 cut(s) 87
BssT1I CCWWGG 1 cut(s) 594
Bst2UI CCWGG 5 cut(s) 70, 84, 392, 457, 636
Bst4CI ACNGT 3 cut(s) 744, 755, 905
Bst6I CTCTTC 1 cut(s) 353
BstACI GRCGYC 1 cut(s) 87
BstAFI CTTAAG 2 cut(s) 607, 665
BstAPI GCANNNNNTGC 2 cut(s) 307, 574
BstC8I GCNNGC 4 cut(s) 75, 517, 702, 837
BstDEI CTNAG 2 cut(s) 29, 765
BstF5I GGATG 1 cut(s) 519
BstFNI CGCG 1 cut(s) 517
BstHHI GCGC 2 cut(s) 517, 519
BstKTI GATC 6 cut(s) 123, 234, 328, 339, 632, 789
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 6 cut(s) 120, 231, 325, 336, 629, 786
BstNI CCWGG 5 cut(s) 70, 84, 392, 457, 636
BstSCI CCNGG 5 cut(s) 68, 82, 390, 455, 634
BstSFI CTRYAG 1 cut(s) 299
BstUI CGCG 1 cut(s) 517
BstV1I GCAGC 7 cut(s) 82, 85, 122, 446, 561, 574, 577
BstV2I GAAGAC 1 cut(s) 834
BstX2I RGATCY 1 cut(s) 629
BstXI CCANNNNNNTGG 1 cut(s) 611
BstYI RGATCY 1 cut(s) 629
BsuRI GGCC 5 cut(s) 41, 77, 346, 455, 750
BtsCI GGATG 1 cut(s) 519
BtsI GCAGTG 1 cut(s) 516
BtsIMutI CAGTG 6 cut(s) 33, 340, 425, 516, 623, 760
Cac8I GCNNGC 4 cut(s) 75, 517, 702, 837
CaiI CAGNNNCTG 1 cut(s) 574
CfoI GCGC 2 cut(s) 517, 519
Cfr13I GGNCC 3 cut(s) 39, 394, 748
CseI GACGC 1 cut(s) 76
Csp6I GTAC 3 cut(s) 294, 414, 879
CviAII CATG 2 cut(s) 260, 578
CviQI GTAC 3 cut(s) 294, 414, 879
DdeI CTNAG 2 cut(s) 29, 765
DpnI GATC 6 cut(s) 122, 233, 327, 338, 631, 788
DpnII GATC 6 cut(s) 120, 231, 325, 336, 629, 786
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 594
Eco24I GRGCYC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 492
Eco47I GGWCC 1 cut(s) 394
Eco53kI GAGCTC 1 cut(s) 28
Eco57I CTGAAG 3 cut(s) 206, 846, 870
EcoICRI GAGCTC 1 cut(s) 28
EcoRI GAATTC 1 cut(s) 581
EcoRII CCWGG 5 cut(s) 68, 82, 390, 455, 634
EcoT14I CCWWGG 1 cut(s) 594
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 2 cut(s) 263, 581
FaiI YATR 8 cut(s) 129, 135, 156, 261, 439, 441, 579, 821
FalI AAGNNNNNCTT 4 cut(s) 782, 814, 819, 851
FatI CATG 2 cut(s) 259, 577
FauI CCCGC 1 cut(s) 210
FauNDI CATATG 1 cut(s) 439
Fnu4HI GCNGC 7 cut(s) 96, 99, 111, 435, 563, 566, 575
FokI GGATG 1 cut(s) 506
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 7 cut(s) 96, 99, 111, 435, 563, 566, 575
FspBI CTAG 2 cut(s) 287, 494
GlaI GCGC 2 cut(s) 516, 518
GluI GCNGC 7 cut(s) 96, 99, 111, 435, 563, 566, 575
GsuI CTGGAG 1 cut(s) 449
HaeIII GGCC 5 cut(s) 41, 77, 346, 455, 750
HapII CCGG 1 cut(s) 730
HgaI GACGC 1 cut(s) 76
HhaI GCGC 2 cut(s) 517, 519
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 2 cut(s) 263, 581
Hin6I GCGC 2 cut(s) 515, 517
HinP1I GCGC 2 cut(s) 515, 517
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HindIII AAGCTT 2 cut(s) 150, 191
HinfI GANTC 6 cut(s) 46, 443, 600, 670, 709, 885
HpaII CCGG 1 cut(s) 730
HphI GGTGA 3 cut(s) 97, 612, 668
Hpy166II GTNNAC 2 cut(s) 604, 892
Hpy188I TCNGA 4 cut(s) 59, 125, 186, 336
Hpy188III TCNNGA 2 cut(s) 323, 352
Hpy8I GTNNAC 2 cut(s) 604, 892
Hpy99I CGWCG 2 cut(s) 92, 95
HpyCH4III ACNGT 3 cut(s) 744, 755, 905
HpyCH4V TGCA 5 cut(s) 263, 301, 310, 577, 839
HpyF3I CTNAG 2 cut(s) 29, 765
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 2 cut(s) 263, 581
HspAI GCGC 2 cut(s) 515, 517
Kzo9I GATC 6 cut(s) 120, 231, 325, 336, 629, 786
Lsp1109I GCAGC 7 cut(s) 82, 85, 122, 446, 561, 574, 577
LweI GCATC 1 cut(s) 528
MaeI CTAG 2 cut(s) 287, 494
MaeIII GTNAC 1 cut(s) 103
MalI GATC 6 cut(s) 122, 233, 327, 338, 631, 788
MboI GATC 6 cut(s) 120, 231, 325, 336, 629, 786
MboII GAAGA 4 cut(s) 199, 281, 370, 839
MflI RGATCY 1 cut(s) 629
MhlI GDGCHC 3 cut(s) 30, 148, 773
MluCI AATT 4 cut(s) 12, 475, 581, 941
MlyI GAGTC 2 cut(s) 609, 703
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 6 cut(s) 214, 424, 534, 564, 858, 916
MseI TTAA 7 cut(s) 117, 378, 534, 608, 651, 666, 873
MslI CAYNNNNRTG 1 cut(s) 609
MspCI CTTAAG 2 cut(s) 607, 665
MspI CCGG 1 cut(s) 730
MspR9I CCNGG 5 cut(s) 70, 84, 392, 457, 636
MvaI CCWGG 5 cut(s) 70, 84, 392, 457, 636
MvnI CGCG 1 cut(s) 517
NdeI CATATG 1 cut(s) 439
NdeII GATC 6 cut(s) 120, 231, 325, 336, 629, 786
NlaIII CATG 2 cut(s) 263, 581
NlaIV GGNNCC 4 cut(s) 40, 67, 631, 907
NmeAIII GCCGAG 1 cut(s) 103
NmuCI GTSAC 1 cut(s) 103
OliI CACNNNNGTG 1 cut(s) 609
PauI GCGCGC 1 cut(s) 515
PcsI WCGNNNNNNNCGW 2 cut(s) 99, 321
PfeI GAWTC 4 cut(s) 46, 443, 670, 885
PflMI CCANNNNNTGG 1 cut(s) 417
PkrI GCNGC 7 cut(s) 97, 100, 112, 436, 564, 567, 576
PleI GAGTC 2 cut(s) 608, 703
PpsI GAGTC 2 cut(s) 608, 703
Psp124BI GAGCTC 1 cut(s) 30
Psp6I CCWGG 5 cut(s) 68, 82, 390, 455, 634
PspGI CCWGG 5 cut(s) 68, 82, 390, 455, 634
PspN4I GGNNCC 4 cut(s) 40, 67, 631, 907
PspPI GGNCC 3 cut(s) 39, 394, 748
PstI CTGCAG 1 cut(s) 303
PstNI CAGNNNCTG 1 cut(s) 574
PsuI RGATCY 1 cut(s) 629
PteI GCGCGC 1 cut(s) 515
RsaI GTAC 3 cut(s) 295, 415, 880
RsaNI GTAC 3 cut(s) 294, 414, 879
RseI CAYNNNNRTG 1 cut(s) 609
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 7 cut(s) 117, 378, 534, 608, 651, 666, 873
SatI GCNGC 7 cut(s) 96, 99, 111, 435, 563, 566, 575
Sau3AI GATC 6 cut(s) 120, 231, 325, 336, 629, 786
Sau96I GGNCC 3 cut(s) 39, 394, 748
SbfI CCTGCAGG 1 cut(s) 303
ScaI AGTACT 1 cut(s) 415
SchI GAGTC 2 cut(s) 609, 703
ScrFI CCNGG 5 cut(s) 70, 84, 392, 457, 636
SdaI CCTGCAGG 1 cut(s) 303
SduI GDGCHC 3 cut(s) 30, 148, 773
SfaNI GCATC 1 cut(s) 528
SfcI CTRYAG 1 cut(s) 299
SinI GGWCC 1 cut(s) 394
SmiMI CAYNNNNRTG 1 cut(s) 609
SmlI CTYRAG 4 cut(s) 147, 607, 665, 717
SmoI CTYRAG 4 cut(s) 147, 607, 665, 717
Sse8387I CCTGCAGG 1 cut(s) 303
Sse9I AATT 4 cut(s) 12, 475, 581, 941
SsiI CCGC 2 cut(s) 108, 217
SspMI CTAG 2 cut(s) 287, 494
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 5 cut(s) 68, 82, 390, 455, 634
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 3 cut(s) 744, 755, 905
TaqI TCGA 3 cut(s) 315, 324, 921
TasI AATT 4 cut(s) 12, 475, 581, 941
TatI WGTACW 1 cut(s) 413
TfiI GAWTC 4 cut(s) 46, 443, 670, 885
Tru1I TTAA 7 cut(s) 117, 378, 534, 608, 651, 666, 873
Tru9I TTAA 7 cut(s) 117, 378, 534, 608, 651, 666, 873
TscAI CASTG 6 cut(s) 40, 347, 432, 516, 630, 760
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 7 cut(s) 95, 98, 110, 434, 562, 565, 574
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 3 cut(s) 177, 371, 594
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 6 cut(s) 40, 347, 432, 516, 630, 760
Van91I CCANNNNNTGG 1 cut(s) 417
Vha464I CTTAAG 2 cut(s) 607, 665
VpaK11BI GGWCC 1 cut(s) 394
XapI RAATTY 1 cut(s) 581
XspI CTAG 2 cut(s) 287, 494
ZrmI AGTACT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.