MD13G1006700.v1.1

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001164
Physical Location & Seq
Forward (+)
424284 .. 427292
3009 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1006700.v1.1.491

Sequence Viewer

Length: 2121 bp
ATGGAGAGAAGCCTTAGCAAAGGCAGTGATACCCAGAGTGACAACTCGGATGCCAAACAGAAGGGCACTGCCGGAAATACCCAAAAGAAGGTGAATGTGGAGGAGGCGTTCAAGTACACGGCGGTGCCGCCCTGGACCAAGCAGATTACGGTGAGGTCGATGGTCACCAGCTTCATCCTCAGCATTGTCTTCAACTTCATCGTCTGCAAGTTGAACCTCACCACCGGCGTCATTCCCTCGCTCAACGTTGCCGCGGGACTGCTCGGTTTCGCGGTGGTGAAAGGCTACACCGCCATGATCGACAAGTGCGGTTTCTTGAAGCAGCCTTTCACTCGCCAGGAAAACACCGTCATCCAGACTTGCGTCGTCGCCTCTTCCGGGATCGCCTTTAGCAGTGGAACTGCAAGTTATTTACTGGGAATGAGTGCAAAGATTGCTGCGCAAGGAGATGAAGGCAACACCCCAATCAACATAAAGAAACTTGATGTTGGATGGATGATTGGGTTTCTCTTCGCTGTCAGCTTCGTCGGGTTGTTTTCGATCATGCCCCTCAGAAAGATGATGATCATGAAGTACAAGTTAACGTACCCGAGTGGAACTGCAACTGCATACCTCATCAACAGCTTTCACACACCCAAAGGGGCTAAGCTGGCAAAGAAACAAGTTGCTGTGCTCTTCAAAACCTTCTGCTTCACCTTTGTGTTTGCCTTTTTCCAGTGGTTTTTCGCTGCTGCTGATGGCTGCGGATTTTCCAGCTTCCCAACATTTGGTCTCAAAGCCTATGCGCACAAGTTTTACTTTGATTTCTCATCGACATATGTTGGTGTAGGCATGATTTGCCCTATCATGGTCAATGTATCTTTGCTTGTTGGAGCCATTATTTCATGGGGAATCATGTGGCCCCTGATTGAGACAAAGAAAGGTATCTGGTACAACGCTAATCTATCCGCCAGCAGTCTCCATGGCATCCAAGGATACAGGGTCGTTTTTATTGCTATAGCCATGATGCTTGGTGATGGTTTATTCCATGTAATCTACATGCTCTTCATGACCACGAAAAGTCTCATGGCACACAGATCAGAGAAGAAACTAGAGACATCATCACCATCCGTAAACCATGACAGTGTTGAAGCGGGAGGGTCCTCAGAAGTTGTGTCTGCAGACTATGGTGAAGAACGAAGAATCGAGTACTTCTTGAAAGACCAAATCCCTAGCTGGGTTGCTTTCTCTGGCTACATTGTGCTGGCAGCCATATCCATCGGTGTAGTACCCTTGATCTTCCCCCAGCTGAAATGGTACCATGTGCTGGTTGCTTATCTGATTGCCCCAGTCTTGGCCTTCTGCAATGCCTACGGCTGTGGCCTCACCGATTGGTCTCTTGCCTCCAACTATGGCAAATTCGCCATCATAATCTTCAGCTCTTGGGTTGGCCTTGACCGTGGTGGTGTCATTGCCGGCCTTGCTTCTTGCGGTGTGACGATGAGCATTGTCTCGACCGCTTCTGATCTCATGCAAGACTTCAAGACAGGATACCTCACGCTCTCTTCTCCTCGCTCTATGTTCTTCAGCCAAGTCTTTGGCACAGCAATGGGATGCGTCATGTCTCCATTAATATTCTGGTTTTTCTACAAAGCTTACTCAATTGGCAATCCAGACGGCCCTTATCCTGCACCCTACGGCCTAATGTACCGCGGCATTGCACTCCTTGGAGTTGAGGGTGTCGGTTCCCTCCCCAAAAACTGCGTGACGCTTGCAGTCTCGTTTTTTGCAGCTGCAGTCGCGATGAACATAATCAGTGAGCTATTGCAGCGTTACGAAACAAAGTACAGGATCCATAGGTTCATTCCGAACCCAATGTGCATGGCCATCCCATTCTACCTGGGTTCCTACTTTGCCATTGACATGTGCGTGGGAAGCTTGATCCTCTACTTGTGGCGGAGCAAGAACAGGCAAAAGGCCAATGACTATGGGCCAGCTGTGGCGTCGGGTCTCATCTGCGGTGAATCTTTGTGGGGCGTTCCGGCTGCTATACTGTCCCTTGCGTCTGTCAAACCTCCTATCTGCATGAAGTTCCTCTCTGCTTCTGTCAACAAGAAAGTTGATTCCCTATTACGTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

707

Amino Acids

77.14

Weight (kDa)

9.26

Isoelectric Point (pI)

32.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 48 - 678 1.5e-155 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 441, 786
Acc65I GGTACC 1 cut(s) 1296
AccB1I GGYRCC 2 cut(s) 124, 1296
AccB7I CCANNNNNTGG 2 cut(s) 767, 1306
AccII CGCG 4 cut(s) 254, 272, 1692, 1781
AclI AACGTT 1 cut(s) 246
AclWI GGATC 4 cut(s) 389, 1825, 1838, 1915
AcoI YGGCCR 1 cut(s) 1863
AcsI RAATTY 1 cut(s) 1397
AcuI CTGAAG 2 cut(s) 1399, 1549
AcyI GRCGYC 2 cut(s) 228, 1982
AfaI GTAC 9 cut(s) 116, 575, 587, 932, 1190, 1269, 1298, 1688, 1826
AfiI CCNNNNNNNGG 7 cut(s) 88, 378, 767, 847, 1216, 1306, 1333
AflIII ACRYGT 1 cut(s) 1902
AgsI TTSAA 8 cut(s) 112, 193, 214, 319, 679, 1130, 1198, 1522
AjnI CCWGG 3 cut(s) 131, 336, 1878
AleI CACNNNNGTG 2 cut(s) 122, 698
AloI GAACNNNNNNTCC 2 cut(s) 92, 124
Alw21I GWGCWC 1 cut(s) 675
AlwI GGATC 4 cut(s) 389, 1825, 1838, 1915
Ama87I CYCGRG 1 cut(s) 589
ApoI RAATTY 1 cut(s) 1397
AseI ATTAAT 1 cut(s) 1610
Asp718I GGTACC 1 cut(s) 1296
AspLEI GCGC 2 cut(s) 442, 787
AspS9I GGNCC 5 cut(s) 135, 900, 1140, 1658, 1970
AsuC2I CCSGG 1 cut(s) 379
AvaI CYCGRG 1 cut(s) 589
AvaII GGWCC 2 cut(s) 135, 1140
BaeGI GKGCMC 1 cut(s) 68
BalI TGGCCA 1 cut(s) 1865
BamHI GGATCC 1 cut(s) 1830
BanI GGYRCC 2 cut(s) 124, 1296
BbsI GAAGAC 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 675
BbvCI CCTCAGC 1 cut(s) 179
BccI CCATC 8 cut(s) 154, 486, 731, 1010, 1114, 1265, 1412, 1874
BceAI ACGGC 4 cut(s) 135, 1369, 1672, 1693
BciT130I CCWGG 3 cut(s) 133, 338, 1880
BciVI GTATCC 2 cut(s) 968, 1523
BclI TGATCA 1 cut(s) 564
BcnI CCSGG 1 cut(s) 379
BfaI CTAG 3 cut(s) 1091, 1212, 2119
BfmI CTRYAG 3 cut(s) 996, 1158, 1773
BfuI GTATCC 2 cut(s) 968, 1523
BlpI GCTNAGC 1 cut(s) 645
BmcAI AGTACT 1 cut(s) 1190
Bme1390I CCNGG 4 cut(s) 133, 338, 379, 1880
Bme18I GGWCC 2 cut(s) 135, 1140
BmeT110I CYCGRG 1 cut(s) 589
BmgT120I GGNCC 5 cut(s) 135, 900, 1140, 1658, 1970
BmiI GGNNCC 8 cut(s) 126, 874, 902, 1141, 1298, 1726, 1832, 1885
BmrFI CCNGG 4 cut(s) 133, 338, 379, 1880
BmrI ACTGGG 2 cut(s) 425, 1322
BmsI GCATC 4 cut(s) 40, 975, 996, 1583
BmuI ACTGGG 2 cut(s) 425, 1322
BoxI GACNNNNGTC 1 cut(s) 362
BpiI GAAGAC 1 cut(s) 181
Bpu10I CCTNAGC 2 cut(s) 14, 179
Bpu1102I GCTNAGC 1 cut(s) 645
BpuMI CCSGG 1 cut(s) 379
BsaAI YACGTR 1 cut(s) 2114
BsaBI GATNNNNATC 1 cut(s) 563
BsaHI GRCGYC 2 cut(s) 228, 1982
BsaI GGTCTC 3 cut(s) 776, 1380, 1994
BsaJI CCNNGG 8 cut(s) 131, 252, 961, 970, 1438, 1690, 1705, 1879
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 7 cut(s) 88, 378, 767, 847, 1216, 1306, 1333
Bse118I RCCGGY 2 cut(s) 224, 1454
Bse1I ACTGG 3 cut(s) 420, 715, 1328
Bse3DI GCAATG 4 cut(s) 1351, 1449, 1593, 1695
Bse8I GATNNNNATC 1 cut(s) 563
BseBI CCWGG 3 cut(s) 133, 338, 1880
BseDI CCNNGG 8 cut(s) 131, 252, 961, 970, 1438, 1690, 1705, 1879
BseGI GGATG 9 cut(s) 55, 174, 351, 497, 501, 966, 1106, 1598, 1866
BseJI GATNNNNATC 1 cut(s) 563
BseLI CCNNNNNNNGG 7 cut(s) 88, 378, 767, 847, 1216, 1306, 1333
BseMI GCAATG 4 cut(s) 1351, 1449, 1593, 1695
BseMII CTCAG 3 cut(s) 193, 565, 1158
BseNI ACTGG 3 cut(s) 420, 715, 1328
BseRI GAGGAG 2 cut(s) 116, 1539
BseSI GKGCMC 1 cut(s) 68
BseYI CCCAGC 2 cut(s) 1215, 1284
BsgI GTGCAG 1 cut(s) 1653
Bsh1236I CGCG 4 cut(s) 254, 272, 1692, 1781
Bsh1285I CGRYCG 1 cut(s) 1497
BshNI GGYRCC 2 cut(s) 124, 1296
BsiEI CGRYCG 1 cut(s) 1497
BsiHKAI GWGCWC 1 cut(s) 675
BsiHKCI CYCGRG 1 cut(s) 589
BsiSI CCGG 5 cut(s) 72, 225, 378, 1455, 2021
BslFI GGGAC 2 cut(s) 270, 2020
BslI CCNNNNNNNGG 7 cut(s) 88, 378, 767, 847, 1216, 1306, 1333
BsmFI GGGAC 2 cut(s) 270, 2020
Bso31I GGTCTC 3 cut(s) 776, 1380, 1994
BsoBI CYCGRG 1 cut(s) 589
Bsp1286I GDGCHC 2 cut(s) 68, 675
Bsp143I GATC 9 cut(s) 297, 381, 540, 564, 1076, 1275, 1504, 1830, 1920
Bsp1720I GCTNAGC 1 cut(s) 645
Bsp19I CCATGG 1 cut(s) 961
Bsp68I TCGCGA 1 cut(s) 1781
BspCNI CTCAG 3 cut(s) 192, 564, 1157
BspFNI CGCG 4 cut(s) 254, 272, 1692, 1781
BspHI TCATGA 2 cut(s) 567, 1047
BspLI GGNNCC 8 cut(s) 126, 874, 902, 1141, 1298, 1726, 1832, 1885
BspMAI CTGCAG 2 cut(s) 1162, 1777
BspPI GGATC 4 cut(s) 389, 1825, 1838, 1915
BspQI GCTCTTC 2 cut(s) 680, 1049
BspT107I GGYRCC 2 cut(s) 124, 1296
BspTNI GGTCTC 3 cut(s) 776, 1380, 1994
BsrDI GCAATG 4 cut(s) 1351, 1449, 1593, 1695
BsrFI RCCGGY 2 cut(s) 224, 1454
BsrI ACTGG 3 cut(s) 420, 715, 1328
BssAI RCCGGY 2 cut(s) 224, 1454
BssECI CCNNGG 8 cut(s) 131, 252, 961, 970, 1438, 1690, 1705, 1879
BssMI GATC 9 cut(s) 297, 381, 540, 564, 1076, 1275, 1504, 1830, 1920
BssNI GRCGYC 2 cut(s) 228, 1982
BssT1I CCWWGG 3 cut(s) 961, 970, 1705
Bst2UI CCWGG 3 cut(s) 133, 338, 1880
Bst4CI ACNGT 5 cut(s) 151, 349, 1124, 1439, 2034
Bst6I CTCTTC 5 cut(s) 379, 515, 680, 1049, 1549
BstACI GRCGYC 2 cut(s) 228, 1982
BstAPI GCANNNNNTGC 2 cut(s) 434, 837
BstBAI YACGTR 1 cut(s) 2114
BstC8I GCNNGC 6 cut(s) 651, 952, 1245, 1456, 1752, 1974
BstDEI CTNAG 5 cut(s) 14, 179, 551, 645, 1144
BstDSI CCRYGG 4 cut(s) 252, 961, 1438, 1690
BstEII GGTNACC 1 cut(s) 163
BstF5I GGATG 9 cut(s) 55, 174, 351, 497, 501, 966, 1106, 1598, 1866
BstFNI CGCG 4 cut(s) 254, 272, 1692, 1781
BstHHI GCGC 2 cut(s) 442, 787
BstKTI GATC 9 cut(s) 300, 384, 543, 567, 1079, 1278, 1507, 1833, 1923
BstMBI GATC 9 cut(s) 297, 381, 540, 564, 1076, 1275, 1504, 1830, 1920
BstMCI CGRYCG 1 cut(s) 1497
BstMWI GCNNNNNNNGC 7 cut(s) 434, 650, 837, 1460, 1778, 1807, 1914
BstNI CCWGG 3 cut(s) 133, 338, 1880
BstNSI RCATGY 2 cut(s) 1042, 1906
BstPAI GACNNNNGTC 1 cut(s) 362
BstPI GGTNACC 1 cut(s) 163
BstSCI CCNGG 4 cut(s) 131, 336, 377, 1878
BstSFI CTRYAG 3 cut(s) 996, 1158, 1773
BstSLI GKGCMC 1 cut(s) 68
BstSNI TACGTA 1 cut(s) 2114
BstUI CGCG 4 cut(s) 254, 272, 1692, 1781
BstV2I GAAGAC 1 cut(s) 181
BstX2I RGATCY 1 cut(s) 1830
BstXI CCANNNNNNTGG 1 cut(s) 1577
BstYI RGATCY 1 cut(s) 1830
BsuI GTATCC 2 cut(s) 968, 1523
BtgI CCRYGG 4 cut(s) 252, 961, 1438, 1690
BtgZI GCGATG 1 cut(s) 1796
BtsCI GGATG 9 cut(s) 55, 174, 351, 497, 501, 966, 1106, 1598, 1866
BtsI GCAGTG 3 cut(s) 31, 66, 400
BtsIMutI CAGTG 6 cut(s) 31, 66, 400, 722, 1129, 1801
BtuMI TCGCGA 1 cut(s) 1781
Cac8I GCNNGC 6 cut(s) 651, 952, 1245, 1456, 1752, 1974
CciI TCATGA 2 cut(s) 567, 1047
CfoI GCGC 2 cut(s) 442, 787
Cfr10I RCCGGY 2 cut(s) 224, 1454
Cfr13I GGNCC 5 cut(s) 135, 900, 1140, 1658, 1970
Cfr42I CCGCGG 2 cut(s) 255, 1693
CseI GACGC 6 cut(s) 217, 352, 1585, 1756, 1971, 2031
Csp6I GTAC 9 cut(s) 115, 574, 586, 931, 1189, 1268, 1297, 1687, 1825
CviQI GTAC 9 cut(s) 115, 574, 586, 931, 1189, 1268, 1297, 1687, 1825
DdeI CTNAG 5 cut(s) 14, 179, 551, 645, 1144
DpnI GATC 9 cut(s) 299, 383, 542, 566, 1078, 1277, 1506, 1832, 1922
DpnII GATC 9 cut(s) 297, 381, 540, 564, 1076, 1275, 1504, 1830, 1920
EaeI YGGCCR 1 cut(s) 1863
Eam1104I CTCTTC 5 cut(s) 379, 515, 680, 1049, 1549
EarI CTCTTC 5 cut(s) 379, 515, 680, 1049, 1549
EciI GGCGGA 2 cut(s) 937, 1951
Eco105I TACGTA 1 cut(s) 2114
Eco130I CCWWGG 3 cut(s) 961, 970, 1705
Eco31I GGTCTC 3 cut(s) 776, 1380, 1994
Eco47I GGWCC 2 cut(s) 135, 1140
Eco57I CTGAAG 2 cut(s) 1399, 1549
Eco88I CYCGRG 1 cut(s) 589
Eco91I GGTNACC 1 cut(s) 163
EcoO109I RGGNCCY 1 cut(s) 1140
EcoO65I GGTNACC 1 cut(s) 163
EcoRII CCWGG 3 cut(s) 131, 336, 1878
EcoT14I CCWWGG 3 cut(s) 961, 970, 1705
ErhI CCWWGG 3 cut(s) 961, 970, 1705
FalI AAGNNNNNCTT 2 cut(s) 782, 814
FaqI GGGAC 2 cut(s) 270, 2020
FauI CCCGC 2 cut(s) 247, 1126
FauNDI CATATG 1 cut(s) 817
FbaI TGATCA 1 cut(s) 564
FokI GGATG 9 cut(s) 62, 161, 338, 504, 508, 953, 1093, 1605, 1853
FspAI RTGCGCAY 1 cut(s) 786
FspBI CTAG 3 cut(s) 1091, 1212, 2119
FspI TGCGCA 2 cut(s) 441, 786
GlaI GCGC 2 cut(s) 441, 786
GsaI CCCAGC 2 cut(s) 1219, 1288
HapII CCGG 5 cut(s) 72, 225, 378, 1455, 2021
HgaI GACGC 6 cut(s) 217, 352, 1585, 1756, 1971, 2031
HhaI GCGC 2 cut(s) 442, 787
Hin1I GRCGYC 2 cut(s) 228, 1982
Hin6I GCGC 2 cut(s) 440, 785
HinP1I GCGC 2 cut(s) 440, 785
HincII GTYRAC 2 cut(s) 582, 2089
HindII GTYRAC 2 cut(s) 582, 2089
HindIII AAGCTT 2 cut(s) 1632, 1915
HinfI GANTC 4 cut(s) 891, 1182, 2003, 2102
HpaI GTTAAC 1 cut(s) 582
HpaII CCGG 5 cut(s) 72, 225, 378, 1455, 2021
Hpy166II GTNNAC 4 cut(s) 117, 582, 1114, 2089
Hpy188I TCNGA 7 cut(s) 49, 554, 1081, 1147, 1320, 1504, 1848
Hpy188III TCNNGA 9 cut(s) 316, 355, 568, 1048, 1195, 1492, 1522, 1652, 1780
Hpy8I GTNNAC 4 cut(s) 117, 582, 1114, 2089
Hpy99I CGWCG 4 cut(s) 368, 371, 530, 1987
HpyAV CCTTC 5 cut(s) 55, 82, 446, 694, 1348
HpyCH4III ACNGT 5 cut(s) 151, 349, 1124, 1439, 2034
HpyCH4IV ACGT 3 cut(s) 246, 584, 2113
HpyF10VI GCNNNNNNNGC 7 cut(s) 434, 650, 837, 1460, 1778, 1807, 1914
HpyF3I CTNAG 5 cut(s) 14, 179, 551, 645, 1144
HpySE526I ACGT 3 cut(s) 246, 584, 2113
Hsp92I GRCGYC 2 cut(s) 228, 1982
HspAI GCGC 2 cut(s) 440, 785
KpnI GGTACC 1 cut(s) 1300
KroI GCCGGC 1 cut(s) 1454
KroNI GCCGGC 1 cut(s) 1456
Ksp22I TGATCA 1 cut(s) 564
KspAI GTTAAC 1 cut(s) 582
KspI CCGCGG 2 cut(s) 255, 1693
Kzo9I GATC 9 cut(s) 297, 381, 540, 564, 1076, 1275, 1504, 1830, 1920
LguI GCTCTTC 2 cut(s) 680, 1049
LmnI GCTCC 2 cut(s) 872, 1938
LweI GCATC 4 cut(s) 40, 975, 996, 1583
MaeI CTAG 3 cut(s) 1091, 1212, 2119
MaeII ACGT 3 cut(s) 246, 584, 2113
MaeIII GTNAC 5 cut(s) 38, 163, 1474, 1744, 1811
MalI GATC 9 cut(s) 299, 383, 542, 566, 1078, 1277, 1506, 1832, 1922
MboI GATC 9 cut(s) 297, 381, 540, 564, 1076, 1275, 1504, 1830, 1920
MfeI CAATTG 1 cut(s) 1641
MflI RGATCY 1 cut(s) 1830
MhlI GDGCHC 2 cut(s) 68, 675
MlsI TGGCCA 1 cut(s) 1865
MluCI AATT 2 cut(s) 1397, 1641
MluNI TGGCCA 1 cut(s) 1865
MmeI TCCRAC 3 cut(s) 469, 850, 1410
Mox20I TGGCCA 1 cut(s) 1865
MroNI GCCGGC 1 cut(s) 1454
MscI TGGCCA 1 cut(s) 1865
MseI TTAA 2 cut(s) 581, 1610
MslI CAYNNNNRTG 7 cut(s) 122, 293, 698, 1122, 1586, 1901, 1907
Msp20I TGGCCA 1 cut(s) 1865
MspA1I CMGCKG 5 cut(s) 254, 1288, 1692, 1772, 1976
MspI CCGG 5 cut(s) 72, 225, 378, 1455, 2021
MspR9I CCNGG 4 cut(s) 133, 338, 379, 1880
MunI CAATTG 1 cut(s) 1641
MvaI CCWGG 3 cut(s) 133, 338, 1880
MvnI CGCG 4 cut(s) 254, 272, 1692, 1781
MwoI GCNNNNNNNGC 7 cut(s) 434, 650, 837, 1460, 1778, 1807, 1914
NaeI GCCGGC 1 cut(s) 1456
NciI CCSGG 1 cut(s) 379
NcoI CCATGG 1 cut(s) 961
NdeI CATATG 1 cut(s) 817
NdeII GATC 9 cut(s) 297, 381, 540, 564, 1076, 1275, 1504, 1830, 1920
NgoMIV GCCGGC 1 cut(s) 1454
NlaIV GGNNCC 8 cut(s) 126, 874, 902, 1141, 1298, 1726, 1832, 1885
NmuCI GTSAC 4 cut(s) 38, 163, 1474, 1744
NruI TCGCGA 1 cut(s) 1781
NsbI TGCGCA 2 cut(s) 441, 786
NspI RCATGY 2 cut(s) 1042, 1906
OliI CACNNNNGTG 2 cut(s) 122, 698
PagI TCATGA 2 cut(s) 567, 1047
PciI ACATGT 1 cut(s) 1902
PciSI GCTCTTC 2 cut(s) 680, 1049
PcsI WCGNNNNNNNCGW 1 cut(s) 155
PdiI GCCGGC 1 cut(s) 1456
PfeI GAWTC 4 cut(s) 891, 1182, 2003, 2102
PflMI CCANNNNNTGG 2 cut(s) 767, 1306
PfoI TCCNGGA 1 cut(s) 377
Ppu21I YACGTR 1 cut(s) 2114
PpuMI RGGWCCY 1 cut(s) 1140
PscI ACATGT 1 cut(s) 1902
PshAI GACNNNNGTC 1 cut(s) 362
PshBI ATTAAT 1 cut(s) 1610
Psp1406I AACGTT 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 1140
Psp6I CCWGG 3 cut(s) 131, 336, 1878
PspEI GGTNACC 1 cut(s) 163
PspFI CCCAGC 2 cut(s) 1215, 1284
PspGI CCWGG 3 cut(s) 131, 336, 1878
PspN4I GGNNCC 8 cut(s) 126, 874, 902, 1141, 1298, 1726, 1832, 1885
PspPI GGNCC 5 cut(s) 135, 900, 1140, 1658, 1970
PspPPI RGGWCCY 1 cut(s) 1140
PstI CTGCAG 2 cut(s) 1162, 1777
PsuI RGATCY 1 cut(s) 1830
PvuII CAGCTG 3 cut(s) 1288, 1772, 1976
RruI TCGCGA 1 cut(s) 1781
RsaI GTAC 9 cut(s) 116, 575, 587, 932, 1190, 1269, 1298, 1688, 1826
RsaNI GTAC 9 cut(s) 115, 574, 586, 931, 1189, 1268, 1297, 1687, 1825
RseI CAYNNNNRTG 7 cut(s) 122, 293, 698, 1122, 1586, 1901, 1907
SacII CCGCGG 2 cut(s) 255, 1693
SapI GCTCTTC 2 cut(s) 680, 1049
SaqAI TTAA 2 cut(s) 581, 1610
Sau3AI GATC 9 cut(s) 297, 381, 540, 564, 1076, 1275, 1504, 1830, 1920
Sau96I GGNCC 5 cut(s) 135, 900, 1140, 1658, 1970
ScaI AGTACT 1 cut(s) 1190
ScrFI CCNGG 4 cut(s) 133, 338, 379, 1880
SduI GDGCHC 2 cut(s) 68, 675
SfaNI GCATC 4 cut(s) 40, 975, 996, 1583
SfcI CTRYAG 3 cut(s) 996, 1158, 1773
Sfr303I CCGCGG 2 cut(s) 255, 1693
SgrAI CRCCGGYG 1 cut(s) 224
SgrBI CCGCGG 2 cut(s) 255, 1693
SinI GGWCC 2 cut(s) 135, 1140
SmiMI CAYNNNNRTG 7 cut(s) 122, 293, 698, 1122, 1586, 1901, 1907
SnaBI TACGTA 1 cut(s) 2114
Sse9I AATT 2 cut(s) 1397, 1641
SspI AATATT 1 cut(s) 1614
SspMI CTAG 3 cut(s) 1091, 1212, 2119
StyD4I CCNGG 4 cut(s) 131, 336, 377, 1878
StyI CCWWGG 3 cut(s) 961, 970, 1705
TaaI ACNGT 5 cut(s) 151, 349, 1124, 1439, 2034
TaiI ACGT 3 cut(s) 249, 587, 2116
TaqI TCGA 6 cut(s) 158, 300, 539, 812, 1185, 1493
TasI AATT 2 cut(s) 1397, 1641
TatI WGTACW 4 cut(s) 114, 573, 1188, 1824
TauI GCSGC 3 cut(s) 130, 254, 1695
TfiI GAWTC 4 cut(s) 891, 1182, 2003, 2102
Tru1I TTAA 2 cut(s) 581, 1610
Tru9I TTAA 2 cut(s) 581, 1610
TscAI CASTG 6 cut(s) 31, 73, 400, 722, 1129, 1801
TseFI GTSAC 4 cut(s) 38, 163, 1474, 1744
Tsp45I GTSAC 4 cut(s) 38, 163, 1474, 1744
TspDTI ATGAA 9 cut(s) 163, 187, 465, 584, 873, 1036, 1799, 1831, 2081
TspGWI ACGGA 1 cut(s) 1099
TspRI CASTG 6 cut(s) 31, 73, 400, 722, 1129, 1801
Van91I CCANNNNNTGG 2 cut(s) 767, 1306
VpaK11BI GGWCC 2 cut(s) 135, 1140
VspI ATTAAT 1 cut(s) 1610
XapI RAATTY 1 cut(s) 1397
XceI RCATGY 2 cut(s) 1042, 1906
XcmI CCANNNNNNNNNTGG 1 cut(s) 1614
XspI CTAG 3 cut(s) 1091, 1212, 2119
ZrmI AGTACT 1 cut(s) 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.