Rw2G024350

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001164
Physical Location & Seq
Forward (+)
35984996 .. 35987174
2179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G024350.1

Sequence Viewer

Length: 2046 bp
ATGGAGACTGACGATTTCGACAATGTTCAAGCTGGAAAACAAATGGTTCAGGCTAGAGACTCGACTGAAAAGGCATTCAGAGACACAGAGGTGCCCCCATGGACCATGCAAATAACGGTCAGGTCTATGGTCACAAGCTTTTTTCTTAGCATCATCTTCAACTTCATCGTGTGCAAGCTCAATCTTACCACCGGTGTAATACCATCTCTAAACGTCGCAGCGGGGCTGTTGGGATTCGCACTGCTGAAAGGATACACTGCCTTGCTTCAAAAGTTAGGTCTGTTGAAGCAGCCTTTCACTCGTCAAGAAAACACTGTGGTTCAAACCTGTGTTGTGGCGTCGTCTGGGATAGCCTTCAGCAGTGGCACGGCGAGTTATTTACTAGGTATGAGCCCTCAGGTAGCTGCTCAGGGAGATGCTGGAAACACTCTAATCAACATTAAGAGGCTTTCGATTCCCTGGATGACAGGATATCTCTTCACAGTCAGCTTCGCTGGCCTTTTTTCGATAATAGCCTTGAGAGAGATGATGATCCTCAAAAGAAAACTGACCTACCCAAGTGGAACAGCAACAGCACACCTCATCAACAGCTTCCACACCCCCAAAGGAGCTAGGATGGCAGCGAGACAAGTTTCTGCTCTCTTCAAGAGCTGCTGCGGCAGCTTTGTCTGGTCTTTTTTCCAGTGGTTTTTCGCAGCTTCGGATGGCTGTGGGTTTGCTAGCTTTCCAATTTTTGGTCTAAAACTCGTTGGGGCTGTGATATCATGGGGAATCATGTGGCCTTTGATTGAACAAAAGAAAGGTATCTGGTATGGAGCTCATCTTTCTGCAAGTAGCATGATACTTGGTGATGGACTATACCATGTTATGTACCATGCTGGTTATATGGGTTTCATTCTCATTAAAAATCTCTATCTCGTTAAAAATCTCTATAGCAACATTGTCATTAAAAATCTCTTTAGCAATAAACCAGAAGAAAAGAAAGTAGAGTTGGCATCGCCTTTGCCGCCACCACTATCATTATCCAATATGGAAGCTGGCGGATTGCCCTCATCAGAAACTGAGCTGAGTGAAAACTACGACGAGGAACGAAGAACTGAGTACTTCTTGAAAGACCAAATTCCACTCTCGGTTGCTGTCGCTGGCTACATTGCTCTTGCAGGCATATCAATTATTGTCCTGCCATTCATTTTCCACCAACTAAAATGGTATCATGTTCTGACAGCTTACTTAATCGCTCCGGTTCTGGCCTTCTGCAATGCCTATGGCTGCGGCCTCACAGATTGGTCCCTTGCTTCCAACTACGGCAAAATCGCAATCCTGATCTTCAGTGCTTGGGTTGGCCTTGAAAAAGGTGGTGTCATTGCTGGCCTCGCTTCTTGTGGTGCAATGATGAGCATTGTCTCGACAGCCTCCGATCTCATGCAAGACTTCAAGACTGGATACCTAACTCTCTCGTCTCCTCGATCCATGTTCTTCAGCCAAGTTTTCGGGACAGCCATGGGGTGTGTCATGTCGCCTTTGATATTCTGGTTCTTCTACAAAGCTTACCGAGTTGGTGATCCCACGGGATCATATCCTGCACCCTATGGCCTAATGTACCGTGGCATTGCACTCCTCGGAGTTGAGGGCTTTGGCTCACTCCCTAAACACTGCGTCACACTTGCAGTCTCCTTTTTCGCATTTGCCATTGCCATTAACATAGTAACCGAGCTTTTGAAGCGTTACGAGACCAAGTACAAGATCTACAGGTTTGTTCCGAATGCAATGGCCATGGCAGTCCCATTCTATCTCGGTTCCTACTTCGTGATTGACATGTGTTTGGGAAGCTTGGTCCTTTTCTGCTGGAAGTTATGGAACAAAAAACAGGCGGAGGATTTGGCACCGGCGGTTGCATCTGGTCTCATCTGTGGCGAGTCTATGTGGGGTGTTCCGGCTGCGATCCTATCTCTGGCCAATGTCAAAGCACCAATATGCATGAAGTTCCTCTCTTACTCGGACAACGATAGAGTTGATTCGTTTTTATCTTCATCAGGATCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

681

Amino Acids

74.63

Weight (kDa)

8.85

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 37 - 248 1.8e-41 OPT oligopeptide transporter protein
OPT PF03169 249 - 649 1.1e-81 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 91, 1882
AccB7I CCANNNNNTGG 1 cut(s) 734
AciI CCGC 7 cut(s) 221, 657, 1007, 1041, 1272, 1871, 1889
AclWI GGATC 6 cut(s) 526, 1461, 1556, 1579, 1936, 2044
AcoI YGGCCR 2 cut(s) 1770, 1953
AcsI RAATTY 1 cut(s) 1119
AcuI CTGAAG 3 cut(s) 340, 1312, 1462
AcyI GRCGYC 1 cut(s) 338
AfaI GTAC 4 cut(s) 872, 1103, 1601, 1739
AfiI CCNNNNNNNGG 2 cut(s) 734, 1951
AflIII ACRYGT 1 cut(s) 1815
AgeI ACCGGT 1 cut(s) 191
AjnI CCWGG 1 cut(s) 458
AjuI GAANNNNNNNTTGG 2 cut(s) 974, 1006
AleI CACNNNNGTG 1 cut(s) 89
Alw21I GWGCWC 1 cut(s) 820
Alw26I GTCTC 8 cut(s) 51, 75, 619, 1408, 1464, 1675, 1724, 1907
AlwI GGATC 6 cut(s) 526, 1461, 1556, 1579, 1936, 2044
AlwNI CAGNNNCTG 1 cut(s) 1061
AoxI GGCC 9 cut(s) 496, 779, 1248, 1273, 1342, 1369, 1591, 1770, 1953
ApoI RAATTY 1 cut(s) 1119
ArsI GACNNNNNNTTYG 2 cut(s) 262, 294
AsiGI ACCGGT 1 cut(s) 191
AspS9I GGNCC 3 cut(s) 102, 1287, 1834
AsuHPI GGTGA 2 cut(s) 860, 1571
AsuNHI GCTAGC 1 cut(s) 719
AvaII GGWCC 3 cut(s) 102, 1287, 1834
AxyI CCTNAGG 1 cut(s) 396
BaeGI GKGCMC 1 cut(s) 96
BalI TGGCCA 2 cut(s) 1772, 1955
BanI GGYRCC 2 cut(s) 91, 1882
BanII GRGCYC 2 cut(s) 395, 820
Bbv12I GWGCWC 1 cut(s) 820
BccI CCATC 4 cut(s) 211, 610, 698, 845
BceAI ACGGC 2 cut(s) 384, 1321
BciT130I CCWGG 1 cut(s) 460
BciVI GTATCC 2 cut(s) 245, 1436
BcoDI GTCTC 8 cut(s) 51, 75, 619, 1408, 1464, 1675, 1724, 1907
BfaI CTAG 6 cut(s) 54, 383, 612, 720, 2040, 2044
BfmI CTRYAG 2 cut(s) 931, 1747
BfuI GTATCC 2 cut(s) 245, 1436
BglII AGATCT 1 cut(s) 1743
BmcAI AGTACT 1 cut(s) 1103
Bme1390I CCNGG 1 cut(s) 460
Bme18I GGWCC 3 cut(s) 102, 1287, 1834
BmgT120I GGNCC 3 cut(s) 102, 1287, 1834
BmiI GGNNCC 4 cut(s) 93, 1289, 1798, 1884
BmrFI CCNGG 1 cut(s) 460
BmsI GCATC 4 cut(s) 159, 406, 1004, 1904
BmtI GCTAGC 1 cut(s) 723
Bpu10I CCTNAGC 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 538
BsaBI GATNNNNATC 1 cut(s) 530
BsaHI GRCGYC 1 cut(s) 338
BsaI GGTCTC 2 cut(s) 1724, 1907
BsaJI CCNNGG 7 cut(s) 98, 458, 1500, 1566, 1603, 1618, 1773
BsaWI WCCGGW 2 cut(s) 191, 1240
Bsc4I CCNNNNNNNGG 2 cut(s) 734, 1951
Bse118I RCCGGY 2 cut(s) 191, 1885
Bse1I ACTGG 2 cut(s) 682, 1444
Bse21I CCTNAGG 1 cut(s) 396
Bse3DI GCAATG 7 cut(s) 1149, 1264, 1362, 1395, 1608, 1689, 1773
Bse8I GATNNNNATC 1 cut(s) 530
BseBI CCWGG 1 cut(s) 460
BseDI CCNNGG 7 cut(s) 98, 458, 1500, 1566, 1603, 1618, 1773
BseGI GGATG 3 cut(s) 468, 621, 709
BseJI GATNNNNATC 1 cut(s) 530
BseLI CCNNNNNNNGG 2 cut(s) 734, 1951
BseMI GCAATG 7 cut(s) 1149, 1264, 1362, 1395, 1608, 1689, 1773
BseMII CTCAG 5 cut(s) 410, 422, 1053, 1058, 1089
BseNI ACTGG 2 cut(s) 682, 1444
BseRI GAGGAG 2 cut(s) 1452, 1607
BseSI GKGCMC 1 cut(s) 96
BsgI GTGCAG 1 cut(s) 1566
BshFI GGCC 9 cut(s) 498, 781, 1250, 1275, 1344, 1371, 1593, 1772, 1955
BshNI GGYRCC 2 cut(s) 91, 1882
BshTI ACCGGT 1 cut(s) 191
BsiHKAI GWGCWC 1 cut(s) 820
BsiSI CCGG 4 cut(s) 192, 1241, 1886, 1934
BslFI GGGAC 3 cut(s) 1273, 1507, 1766
BslI CCNNNNNNNGG 2 cut(s) 734, 1951
BsmAI GTCTC 8 cut(s) 51, 75, 619, 1408, 1464, 1675, 1724, 1907
BsmBI CGTCTC 1 cut(s) 1464
BsmFI GGGAC 3 cut(s) 1273, 1507, 1766
BsmI GAATGC 2 cut(s) 74, 1768
BsnI GGCC 9 cut(s) 498, 781, 1250, 1275, 1344, 1371, 1593, 1772, 1955
Bso31I GGTCTC 2 cut(s) 1724, 1907
Bsp1286I GDGCHC 3 cut(s) 96, 395, 820
Bsp143I GATC 9 cut(s) 531, 1323, 1417, 1466, 1561, 1571, 1743, 1941, 2036
Bsp19I CCATGG 3 cut(s) 98, 1500, 1773
BspACI CCGC 7 cut(s) 221, 657, 1007, 1041, 1272, 1871, 1889
BspANI GGCC 9 cut(s) 498, 781, 1250, 1275, 1344, 1371, 1593, 1772, 1955
BspCNI CTCAG 5 cut(s) 409, 421, 1054, 1059, 1090
BspLI GGNNCC 4 cut(s) 93, 1289, 1798, 1884
BspOI GCTAGC 1 cut(s) 723
BspPI GGATC 6 cut(s) 526, 1461, 1556, 1579, 1936, 2044
BspT107I GGYRCC 2 cut(s) 91, 1882
BspTNI GGTCTC 2 cut(s) 1724, 1907
BsrDI GCAATG 7 cut(s) 1149, 1264, 1362, 1395, 1608, 1689, 1773
BsrFI RCCGGY 2 cut(s) 191, 1885
BsrI ACTGG 2 cut(s) 682, 1444
BssAI RCCGGY 2 cut(s) 191, 1885
BssECI CCNNGG 7 cut(s) 98, 458, 1500, 1566, 1603, 1618, 1773
BssMI GATC 9 cut(s) 531, 1323, 1417, 1466, 1561, 1571, 1743, 1941, 2036
BssNI GRCGYC 1 cut(s) 338
BssT1I CCWWGG 3 cut(s) 98, 1500, 1773
Bst2UI CCWGG 1 cut(s) 460
Bst4CI ACNGT 4 cut(s) 118, 316, 484, 1604
Bst6I CTCTTC 2 cut(s) 482, 647
BstACI GRCGYC 1 cut(s) 338
BstC8I GCNNGC 7 cut(s) 176, 496, 721, 1039, 1144, 1162, 1369
BstDEI CTNAG 6 cut(s) 146, 396, 408, 1062, 1067, 1098
BstDSI CCRYGG 5 cut(s) 98, 1500, 1566, 1603, 1773
BstF5I GGATG 3 cut(s) 468, 621, 709
BstKTI GATC 9 cut(s) 534, 1326, 1420, 1469, 1564, 1574, 1746, 1944, 2039
BstMAI GTCTC 8 cut(s) 51, 75, 619, 1408, 1464, 1675, 1724, 1907
BstMBI GATC 9 cut(s) 531, 1323, 1417, 1466, 1561, 1571, 1743, 1941, 2036
BstMWI GCNNNNNNNGC 7 cut(s) 495, 617, 657, 660, 1006, 1373, 1720
BstNI CCWGG 1 cut(s) 460
BstNSI RCATGY 1 cut(s) 1819
BstSCI CCNGG 1 cut(s) 458
BstSFI CTRYAG 2 cut(s) 931, 1747
BstSLI GKGCMC 1 cut(s) 96
BstX2I RGATCY 2 cut(s) 1743, 2036
BstYI RGATCY 2 cut(s) 1743, 2036
Bsu36I CCTNAGG 1 cut(s) 396
BsuI GTATCC 2 cut(s) 245, 1436
BsuRI GGCC 9 cut(s) 498, 781, 1250, 1275, 1344, 1371, 1593, 1772, 1955
BtgI CCRYGG 5 cut(s) 98, 1500, 1566, 1603, 1773
BtgZI GCGATG 1 cut(s) 981
BtsCI GGATG 3 cut(s) 468, 621, 709
BtsI GCAGTG 4 cut(s) 239, 255, 367, 1651
BtsIMutI CAGTG 7 cut(s) 239, 255, 312, 367, 689, 1336, 1651
Cac8I GCNNGC 7 cut(s) 176, 496, 721, 1039, 1144, 1162, 1369
CaiI CAGNNNCTG 1 cut(s) 1061
Cfr10I RCCGGY 2 cut(s) 191, 1885
Cfr13I GGNCC 3 cut(s) 102, 1287, 1834
CseI GACGC 2 cut(s) 327, 1645
Csp6I GTAC 4 cut(s) 871, 1102, 1600, 1738
CspAI ACCGGT 1 cut(s) 191
CviQI GTAC 4 cut(s) 871, 1102, 1600, 1738
DdeI CTNAG 6 cut(s) 146, 396, 408, 1062, 1067, 1098
DpnI GATC 9 cut(s) 533, 1325, 1419, 1468, 1563, 1573, 1745, 1943, 2038
DpnII GATC 9 cut(s) 531, 1323, 1417, 1466, 1561, 1571, 1743, 1941, 2036
EaeI YGGCCR 2 cut(s) 1770, 1953
Eam1104I CTCTTC 2 cut(s) 482, 647
EarI CTCTTC 2 cut(s) 482, 647
EciI GGCGGA 2 cut(s) 1056, 1886
Ecl136II GAGCTC 1 cut(s) 818
Eco130I CCWWGG 3 cut(s) 98, 1500, 1773
Eco24I GRGCYC 2 cut(s) 395, 820
Eco31I GGTCTC 2 cut(s) 1724, 1907
Eco32I GATATC 2 cut(s) 473, 762
Eco47I GGWCC 3 cut(s) 102, 1287, 1834
Eco53kI GAGCTC 1 cut(s) 818
Eco57I CTGAAG 3 cut(s) 340, 1312, 1462
Eco81I CCTNAGG 1 cut(s) 396
EcoICRI GAGCTC 1 cut(s) 818
EcoRII CCWGG 1 cut(s) 458
EcoRV GATATC 2 cut(s) 473, 762
EcoT14I CCWWGG 3 cut(s) 98, 1500, 1773
EcoT22I ATGCAT 1 cut(s) 1979
EcoT38I GRGCYC 2 cut(s) 395, 820
ErhI CCWWGG 3 cut(s) 98, 1500, 1773
Esp3I CGTCTC 1 cut(s) 1464
FaqI GGGAC 3 cut(s) 1273, 1507, 1766
FauI CCCGC 1 cut(s) 214
FokI GGATG 3 cut(s) 475, 628, 716
FriOI GRGCYC 2 cut(s) 395, 820
FspBI CTAG 6 cut(s) 54, 383, 612, 720, 2040, 2044
HaeIII GGCC 9 cut(s) 498, 781, 1250, 1275, 1344, 1371, 1593, 1772, 1955
HapII CCGG 4 cut(s) 192, 1241, 1886, 1934
HgaI GACGC 2 cut(s) 327, 1645
Hin1I GRCGYC 1 cut(s) 338
HindIII AAGCTT 3 cut(s) 136, 1545, 1828
HinfI GANTC 6 cut(s) 59, 234, 454, 771, 1916, 2015
HpaII CCGG 4 cut(s) 192, 1241, 1886, 1934
HphI GGTGA 2 cut(s) 860, 1571
Hpy188I TCNGA 8 cut(s) 80, 703, 1057, 1221, 1417, 1622, 1761, 1999
Hpy188III TCNNGA 9 cut(s) 305, 646, 1108, 1321, 1405, 1435, 1492, 1807, 2034
Hpy99I CGWCG 3 cut(s) 218, 343, 1085
HpyAV CCTTC 2 cut(s) 364, 1261
HpyCH4III ACNGT 4 cut(s) 118, 316, 484, 1604
HpyCH4IV ACGT 1 cut(s) 213
HpyF10VI GCNNNNNNNGC 7 cut(s) 495, 617, 657, 660, 1006, 1373, 1720
HpyF3I CTNAG 6 cut(s) 146, 396, 408, 1062, 1067, 1098
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 1 cut(s) 338
Kzo9I GATC 9 cut(s) 531, 1323, 1417, 1466, 1561, 1571, 1743, 1941, 2036
LmnI GCTCC 3 cut(s) 608, 815, 1243
LweI GCATC 4 cut(s) 159, 406, 1004, 1904
MaeI CTAG 6 cut(s) 54, 383, 612, 720, 2040, 2044
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 4 cut(s) 130, 1657, 1705, 1724
MalI GATC 9 cut(s) 533, 1325, 1419, 1468, 1563, 1573, 1745, 1943, 2038
MboI GATC 9 cut(s) 531, 1323, 1417, 1466, 1561, 1571, 1743, 1941, 2036
MboII GAAGA 9 cut(s) 148, 469, 634, 986, 1104, 1318, 1468, 1528, 2019
MflI RGATCY 2 cut(s) 1743, 2036
MhlI GDGCHC 3 cut(s) 96, 395, 820
MlsI TGGCCA 2 cut(s) 1772, 1955
MluCI AATT 3 cut(s) 729, 1119, 1170
MluNI TGGCCA 2 cut(s) 1772, 1955
MlyI GAGTC 2 cut(s) 53, 1925
MmeI TCCRAC 1 cut(s) 1323
Mox20I TGGCCA 2 cut(s) 1772, 1955
Mph1103I ATGCAT 1 cut(s) 1979
MscI TGGCCA 2 cut(s) 1772, 1955
MseI TTAA 6 cut(s) 441, 903, 921, 948, 1232, 1698
MslI CAYNNNNRTG 2 cut(s) 89, 1972
Msp20I TGGCCA 2 cut(s) 1772, 1955
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 4 cut(s) 192, 1241, 1886, 1934
MspR9I CCNGG 1 cut(s) 460
Mva1269I GAATGC 2 cut(s) 74, 1768
MvaI CCWGG 1 cut(s) 460
MwoI GCNNNNNNNGC 7 cut(s) 495, 617, 657, 660, 1006, 1373, 1720
NcoI CCATGG 3 cut(s) 98, 1500, 1773
NdeII GATC 9 cut(s) 531, 1323, 1417, 1466, 1561, 1571, 1743, 1941, 2036
NheI GCTAGC 1 cut(s) 719
NlaIV GGNNCC 4 cut(s) 93, 1289, 1798, 1884
NmuCI GTSAC 2 cut(s) 130, 1657
NsiI ATGCAT 1 cut(s) 1979
NspI RCATGY 1 cut(s) 1819
OliI CACNNNNGTG 1 cut(s) 89
PciI ACATGT 1 cut(s) 1815
PctI GAATGC 2 cut(s) 74, 1768
PfeI GAWTC 4 cut(s) 234, 454, 771, 2015
PflMI CCANNNNNTGG 1 cut(s) 734
PinAI ACCGGT 1 cut(s) 191
PleI GAGTC 2 cut(s) 53, 1924
PpsI GAGTC 2 cut(s) 53, 1924
PscI ACATGT 1 cut(s) 1815
Psp124BI GAGCTC 1 cut(s) 820
Psp6I CCWGG 1 cut(s) 458
PspGI CCWGG 1 cut(s) 458
PspN4I GGNNCC 4 cut(s) 93, 1289, 1798, 1884
PspPI GGNCC 3 cut(s) 102, 1287, 1834
PstNI CAGNNNCTG 1 cut(s) 1061
PsuI RGATCY 2 cut(s) 1743, 2036
RsaI GTAC 4 cut(s) 872, 1103, 1601, 1739
RsaNI GTAC 4 cut(s) 871, 1102, 1600, 1738
RseI CAYNNNNRTG 2 cut(s) 89, 1972
SacI GAGCTC 1 cut(s) 820
SaqAI TTAA 6 cut(s) 441, 903, 921, 948, 1232, 1698
Sau3AI GATC 9 cut(s) 531, 1323, 1417, 1466, 1561, 1571, 1743, 1941, 2036
Sau96I GGNCC 3 cut(s) 102, 1287, 1834
ScaI AGTACT 1 cut(s) 1103
SchI GAGTC 2 cut(s) 53, 1925
ScrFI CCNGG 1 cut(s) 460
SduI GDGCHC 3 cut(s) 96, 395, 820
SfaNI GCATC 4 cut(s) 159, 406, 1004, 1904
SfcI CTRYAG 2 cut(s) 931, 1747
SgrAI CRCCGGYG 2 cut(s) 191, 1885
SinI GGWCC 3 cut(s) 102, 1287, 1834
SmiMI CAYNNNNRTG 2 cut(s) 89, 1972
SmlI CTYRAG 1 cut(s) 517
SmoI CTYRAG 1 cut(s) 517
Sse9I AATT 3 cut(s) 729, 1119, 1170
SsiI CCGC 7 cut(s) 221, 657, 1007, 1041, 1272, 1871, 1889
SspMI CTAG 6 cut(s) 54, 383, 612, 720, 2040, 2044
SstI GAGCTC 1 cut(s) 820
StyD4I CCNGG 1 cut(s) 458
StyI CCWWGG 3 cut(s) 98, 1500, 1773
TaaI ACNGT 4 cut(s) 118, 316, 484, 1604
TaiI ACGT 1 cut(s) 216
TaqI TCGA 6 cut(s) 18, 62, 452, 506, 1406, 1465
TasI AATT 3 cut(s) 729, 1119, 1170
TatI WGTACW 2 cut(s) 1101, 1737
TauI GCSGC 3 cut(s) 660, 1009, 1275
TfiI GAWTC 4 cut(s) 234, 454, 771, 2015
Tru1I TTAA 6 cut(s) 441, 903, 921, 948, 1232, 1698
Tru9I TTAA 6 cut(s) 441, 903, 921, 948, 1232, 1698
TscAI CASTG 7 cut(s) 246, 262, 319, 367, 689, 1336, 1658
TseFI GTSAC 2 cut(s) 130, 1657
Tsp45I GTSAC 2 cut(s) 130, 1657
TspDTI ATGAA 5 cut(s) 154, 883, 1177, 1994, 2019
TspRI CASTG 7 cut(s) 246, 262, 319, 367, 689, 1336, 1658
Van91I CCANNNNNTGG 1 cut(s) 734
VpaK11BI GGWCC 3 cut(s) 102, 1287, 1834
XapI RAATTY 1 cut(s) 1119
XceI RCATGY 1 cut(s) 1819
XspI CTAG 6 cut(s) 54, 383, 612, 720, 2040, 2044
ZrmI AGTACT 1 cut(s) 1103
Zsp2I ATGCAT 1 cut(s) 1979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.