Rroxscaffold_5G00386960

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001164
Physical Location & Seq
Reverse (-)
65685658 .. 65687530
1873 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386960.1

Sequence Viewer

Length: 1704 bp
ATGAGTGGAACGATAGCTGCTCAAGGAGATGAAGGCAACACCCCAATCAACGTGAAGAACCCTCACATTGGATGGATGATTGGATTTCTCTTTACTGTCAGCTTCCTCGGCTTGTTTTCCATTATGCCGATGAGAAAGATGATGATCATGGAGTACAAGTTAACCTACCCAAGTGGAACTGCAACTGCATACCTCATTAACAGTTTTCACACCCCCAAAGGAGCCAAGTTAGCAAAGAAACAAGTTGGCGTCCTGTTCAAAAGCTTCTGCTTTAGCTTTGTGTGGGCCTTTTTCCAATGGTTCTACACAGCCGCTGATGGCTGCGGATTTGCTAGCTTCCCAACATTTGGTCTCCAAGCTTATGCACAGAAGTTTTACTTTGACTTCTCATCGACATACATTGGTGTGGGAATGATTTGCCCTTACATGGTGAATATCTCTTTGCTTGTCGGATCAATACTTTCATGGGGAATCATGTGGCCGTTGATTGAGAAACATAAAGGTACCTGGTACAGTGCTGATCTCTCAGCGAGCAGTCTCCACGGTATCCAAGGTTACAGGGTTTTCATTGCTATAGCCATGATGCTTGGGGATGGTTTATACCATGTAGCTTACATGCTCCTTGTGACCTTCCAAAATTTATGTTGTACACCTAAAACAAAAGAAGAAATGAAATTAGAGTCATCATCCCCACCGTCCATAAACCCTGCAAGTACTGAAGCTGGAGTAGAAGTTTTGAGTGAAAACTATGACGAGGAGCGAAGAGTTGAGTATTTCTTGAAAGACGCAATTTCTCACTGGGTTGCTCTCGGTGGATATGTTGCCCTTGCCATTATATCCATCATTACCGTACCAAGCATTTTCCGCCAATTAAAATGGTACCATATCTTTGTTGCTTATTTGGTTGCTCCAATTTTGGCATTCTGTAATTCCTATGGTGCTGGTCTCACCGATTGGTCCCTCGCCTCCAACTATGGAAAATTCGCCATCATAATCTTCAGTGCTTGGGTTGGCCCTGAAGGTGGTGTTGTTGCTGGGCTTGCTGCTTGTGGTGTCATGATGAGCATTGTCGCTACTGCTTCTGATCTTATGCAGGACTTCAAGACTGGATACCTTACGCTTTCCTCTCCTCGCTCTATGTTCTTCAGCCAAGTTCTTGGCACGGCCATGGGTTGTCTCATGTCCCCTTTGGTATTCTGGATTTTCTACAAAGCTTATCCACTAGGCGATCCAAATGGTTCGTATCCTGCACCGTTTGCACTAATGTACCGTGGAATGGCTCTTTTAGGAGTTGAAGGCGTTGGTTCACTTCCCAAAAATTGTGTGAAACTTGCAGTCTCATTTTTTGTAGCTGCTGTGGTCATAAATTTGATTACTGAGCTTCTAAAGCGTTATGAGACCAAGTACAGGTTGTACAGATTTATTCCTAGCCCAATGTGCATGGCCATCCCATTTTATCTTGGTTCATACTTTGCCATTGACATGTGTTTGGGGAGCTTGATTCTCTTCTTGTGGCAAAGGAAGAACAAACAAAAGGCCGATGACTTTGCACCAGCAGTCGCATCTGGACTCATTTGCGGTGAATCTTTGTGGGGTATTCCAGCTGCTATCCTTTCACTGGCAAATATGAAACCTCCGATCTGTATGAAGTTCCTCTCTGCTTCTGCCAATGAAAAAGTCGAAAAACTATTATCTGGTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

567

Amino Acids

62.52

Weight (kDa)

8.69

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 19 - 538 1.7e-135 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 503, 879
AccB1I GGYRCC 2 cut(s) 503, 879
AccB7I CCANNNNNTGG 1 cut(s) 347
AciI CCGC 4 cut(s) 312, 324, 865, 1578
AclWI GGATC 2 cut(s) 460, 1223
AcoI YGGCCR 3 cut(s) 479, 1164, 1443
AcsI RAATTY 3 cut(s) 637, 980, 1366
AcuI CTGAAG 4 cut(s) 738, 982, 1038, 1129
AcyI GRCGYC 1 cut(s) 249
AfiI CCNNNNNNNGG 7 cut(s) 68, 347, 427, 1022, 1276, 1407, 1618
AflIII ACRYGT 1 cut(s) 1482
AgsI TTSAA 4 cut(s) 259, 781, 1102, 1295
AjnI CCWGG 1 cut(s) 506
Alw26I GTCTC 6 cut(s) 356, 542, 950, 1181, 1342, 1391
AlwI GGATC 2 cut(s) 460, 1223
AlwNI CAGNNNCTG 1 cut(s) 314
AoxI GGCC 6 cut(s) 285, 479, 1012, 1164, 1443, 1536
ApeKI GCWGC 5 cut(s) 17, 321, 1043, 1352, 1604
ApoI RAATTY 3 cut(s) 637, 980, 1366
Asp718I GGTACC 2 cut(s) 503, 879
AspS9I GGNCC 3 cut(s) 285, 957, 1013
AsuHPI GGTGA 3 cut(s) 442, 940, 1592
AsuNHI GCTAGC 1 cut(s) 332
AvaII GGWCC 1 cut(s) 957
BaeI ACNNNNGTAYC 2 cut(s) 1250, 1283
BalI TGGCCA 1 cut(s) 1445
BanI GGYRCC 2 cut(s) 503, 879
BbvI GCAGC 5 cut(s) 4, 308, 1030, 1339, 1591
BccI CCATC 6 cut(s) 66, 311, 587, 848, 995, 1454
BceAI ACGGC 2 cut(s) 466, 1179
BcgI CGANNNNNNTGC 2 cut(s) 1529, 1563
BciT130I CCWGG 1 cut(s) 508
BciVI GTATCC 3 cut(s) 557, 1103, 1254
BclI TGATCA 1 cut(s) 144
BcoDI GTCTC 6 cut(s) 356, 542, 950, 1181, 1342, 1391
BfaI CTAG 3 cut(s) 333, 1223, 1428
BfmI CTRYAG 1 cut(s) 573
BfuI GTATCC 3 cut(s) 557, 1103, 1254
BisI GCNGC 6 cut(s) 18, 312, 322, 1044, 1353, 1605
BlsI GCNGC 6 cut(s) 19, 313, 323, 1045, 1354, 1606
BmcAI AGTACT 1 cut(s) 715
Bme1390I CCNGG 1 cut(s) 508
Bme18I GGWCC 1 cut(s) 957
BmgT120I GGNCC 3 cut(s) 285, 957, 1013
BmiI GGNNCC 4 cut(s) 223, 505, 881, 959
BmrFI CCNGG 1 cut(s) 508
BmrI ACTGGG 1 cut(s) 808
BmsI GCATC 2 cut(s) 573, 1571
BmtI GCTAGC 1 cut(s) 336
BmuI ACTGGG 1 cut(s) 808
BpmI CTGGAG 1 cut(s) 744
BpuEI CTTGAG 1 cut(s) 6
BsaBI GATNNNNATC 1 cut(s) 143
BsaHI GRCGYC 1 cut(s) 249
BsaI GGTCTC 3 cut(s) 356, 950, 1391
BsaJI CCNNGG 5 cut(s) 106, 541, 550, 1167, 1270
BsaXI ACNNNNNCTCC 4 cut(s) 143, 173, 717, 747
Bsc4I CCNNNNNNNGG 7 cut(s) 68, 347, 427, 1022, 1276, 1407, 1618
Bse1I ACTGG 3 cut(s) 803, 1111, 1623
Bse3DI GCAATG 1 cut(s) 567
Bse8I GATNNNNATC 1 cut(s) 143
BseBI CCWGG 1 cut(s) 508
BseDI CCNNGG 5 cut(s) 106, 541, 550, 1167, 1270
BseGI GGATG 5 cut(s) 77, 81, 598, 686, 1446
BseJI GATNNNNATC 1 cut(s) 143
BseLI CCNNNNNNNGG 7 cut(s) 68, 347, 427, 1022, 1276, 1407, 1618
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 2 cut(s) 540, 1368
BseNI ACTGG 3 cut(s) 803, 1111, 1623
BseRI GAGGAG 2 cut(s) 770, 1119
BseXI GCAGC 5 cut(s) 4, 308, 1030, 1339, 1591
BseYI CCCAGC 1 cut(s) 1034
BsgI GTGCAG 1 cut(s) 1233
BshFI GGCC 6 cut(s) 287, 481, 1014, 1166, 1445, 1538
BshNI GGYRCC 2 cut(s) 503, 879
BslFI GGGAC 2 cut(s) 943, 1168
BslI CCNNNNNNNGG 7 cut(s) 68, 347, 427, 1022, 1276, 1407, 1618
BsmAI GTCTC 6 cut(s) 356, 542, 950, 1181, 1342, 1391
BsmFI GGGAC 2 cut(s) 943, 1168
BsmI GAATGC 1 cut(s) 920
BsnI GGCC 6 cut(s) 287, 481, 1014, 1166, 1445, 1538
Bso31I GGTCTC 3 cut(s) 356, 950, 1391
Bsp1407I TGTACA 2 cut(s) 647, 1413
Bsp143I GATC 6 cut(s) 144, 452, 520, 1084, 1228, 1638
Bsp19I CCATGG 1 cut(s) 1167
BspACI CCGC 4 cut(s) 312, 324, 865, 1578
BspANI GGCC 6 cut(s) 287, 481, 1014, 1166, 1445, 1538
BspCNI CTCAG 2 cut(s) 539, 1369
BspHI TCATGA 1 cut(s) 1056
BspLI GGNNCC 4 cut(s) 223, 505, 881, 959
BspOI GCTAGC 1 cut(s) 336
BspPI GGATC 2 cut(s) 460, 1223
BspT107I GGYRCC 2 cut(s) 503, 879
BspTNI GGTCTC 3 cut(s) 356, 950, 1391
BsrDI GCAATG 1 cut(s) 567
BsrGI TGTACA 2 cut(s) 647, 1413
BsrI ACTGG 3 cut(s) 803, 1111, 1623
BssECI CCNNGG 5 cut(s) 106, 541, 550, 1167, 1270
BssMI GATC 6 cut(s) 144, 452, 520, 1084, 1228, 1638
BssNI GRCGYC 1 cut(s) 249
BssT1I CCWWGG 2 cut(s) 550, 1167
Bst2UI CCWGG 1 cut(s) 508
Bst4CI ACNGT 8 cut(s) 97, 203, 515, 545, 696, 850, 1254, 1271
Bst6I CTCTTC 2 cut(s) 757, 1511
BstACI GRCGYC 1 cut(s) 249
BstAPI GCANNNNNTGC 1 cut(s) 1256
BstAUI TGTACA 2 cut(s) 647, 1413
BstC8I GCNNGC 3 cut(s) 334, 532, 1041
BstDEI CTNAG 2 cut(s) 526, 1377
BstDSI CCRYGG 3 cut(s) 541, 1167, 1270
BstF5I GGATG 5 cut(s) 77, 81, 598, 686, 1446
BstKTI GATC 6 cut(s) 147, 455, 523, 1087, 1231, 1641
BstMAI GTCTC 6 cut(s) 356, 542, 950, 1181, 1342, 1391
BstMBI GATC 6 cut(s) 144, 452, 520, 1084, 1228, 1638
BstMWI GCNNNNNNNGC 7 cut(s) 108, 230, 864, 1040, 1256, 1387, 1437
BstNI CCWGG 1 cut(s) 508
BstNSI RCATGY 2 cut(s) 619, 1486
BstSCI CCNGG 1 cut(s) 506
BstSFI CTRYAG 1 cut(s) 573
BstV1I GCAGC 5 cut(s) 4, 308, 1030, 1339, 1591
BstXI CCANNNNNNTGG 1 cut(s) 1157
BsuI GTATCC 3 cut(s) 557, 1103, 1254
BsuRI GGCC 6 cut(s) 287, 481, 1014, 1166, 1445, 1538
BtgI CCRYGG 3 cut(s) 541, 1167, 1270
BtsCI GGATG 5 cut(s) 77, 81, 598, 686, 1446
BtsIMutI CAGTG 4 cut(s) 520, 796, 1006, 1616
Cac8I GCNNGC 3 cut(s) 334, 532, 1041
CaiI CAGNNNCTG 1 cut(s) 314
CciI TCATGA 1 cut(s) 1056
Cfr13I GGNCC 3 cut(s) 285, 957, 1013
CseI GACGC 2 cut(s) 238, 794
CsiI ACCWGGT 1 cut(s) 506
DdeI CTNAG 2 cut(s) 526, 1377
DpnI GATC 6 cut(s) 146, 454, 522, 1086, 1230, 1640
DpnII GATC 6 cut(s) 144, 452, 520, 1084, 1228, 1638
EaeI YGGCCR 3 cut(s) 479, 1164, 1443
Eam1104I CTCTTC 2 cut(s) 757, 1511
EarI CTCTTC 2 cut(s) 757, 1511
EciI GGCGGA 1 cut(s) 854
Eco130I CCWWGG 2 cut(s) 550, 1167
Eco31I GGTCTC 3 cut(s) 356, 950, 1391
Eco47I GGWCC 1 cut(s) 957
Eco57I CTGAAG 4 cut(s) 738, 982, 1038, 1129
EcoRII CCWGG 1 cut(s) 506
EcoT14I CCWWGG 2 cut(s) 550, 1167
ErhI CCWWGG 2 cut(s) 550, 1167
FalI AAGNNNNNCTT 2 cut(s) 362, 394
FaqI GGGAC 2 cut(s) 943, 1168
FbaI TGATCA 1 cut(s) 144
Fnu4HI GCNGC 6 cut(s) 18, 312, 322, 1044, 1353, 1605
FokI GGATG 5 cut(s) 84, 88, 605, 673, 1433
Fsp4HI GCNGC 6 cut(s) 18, 312, 322, 1044, 1353, 1605
FspBI CTAG 3 cut(s) 333, 1223, 1428
GluI GCNGC 6 cut(s) 18, 312, 322, 1044, 1353, 1605
GsaI CCCAGC 1 cut(s) 1038
GsuI CTGGAG 1 cut(s) 744
HaeIII GGCC 6 cut(s) 287, 481, 1014, 1166, 1445, 1538
HgaI GACGC 2 cut(s) 238, 794
Hin1I GRCGYC 1 cut(s) 249
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HindIII AAGCTT 3 cut(s) 262, 357, 1212
HinfI GANTC 5 cut(s) 471, 680, 1501, 1569, 1583
HpaI GTTAAC 1 cut(s) 162
HphI GGTGA 3 cut(s) 442, 940, 1592
Hpy166II GTNNAC 3 cut(s) 162, 650, 1307
Hpy188I TCNGA 3 cut(s) 452, 1084, 1638
Hpy188III TCNNGA 5 cut(s) 778, 1057, 1102, 1198, 1566
Hpy8I GTNNAC 3 cut(s) 162, 650, 1307
HpyAV CCTTC 4 cut(s) 26, 640, 1013, 1289
HpyCH4III ACNGT 8 cut(s) 97, 203, 515, 545, 696, 850, 1254, 1271
HpyCH4IV ACGT 1 cut(s) 51
HpyF10VI GCNNNNNNNGC 7 cut(s) 108, 230, 864, 1040, 1256, 1387, 1437
HpyF3I CTNAG 2 cut(s) 526, 1377
HpySE526I ACGT 1 cut(s) 51
Hsp92I GRCGYC 1 cut(s) 249
KpnI GGTACC 2 cut(s) 507, 883
Ksp22I TGATCA 1 cut(s) 144
KspAI GTTAAC 1 cut(s) 162
Kzo9I GATC 6 cut(s) 144, 452, 520, 1084, 1228, 1638
LmnI GCTCC 5 cut(s) 221, 624, 757, 913, 1494
Lsp1109I GCAGC 5 cut(s) 4, 308, 1030, 1339, 1591
LweI GCATC 2 cut(s) 573, 1571
MabI ACCWGGT 1 cut(s) 506
MaeI CTAG 3 cut(s) 333, 1223, 1428
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 2 cut(s) 554, 625
MalI GATC 6 cut(s) 146, 454, 522, 1086, 1230, 1640
MboI GATC 6 cut(s) 144, 452, 520, 1084, 1228, 1638
MboII GAAGA 7 cut(s) 67, 677, 774, 988, 1135, 1498, 1534
MlsI TGGCCA 1 cut(s) 1445
MluCI AATT 9 cut(s) 637, 674, 789, 869, 912, 928, 980, 1318, 1366
MluNI TGGCCA 1 cut(s) 1445
MlyI GAGTC 2 cut(s) 689, 1563
MmeI TCCRAC 2 cut(s) 430, 993
Mox20I TGGCCA 1 cut(s) 1445
MscI TGGCCA 1 cut(s) 1445
MseI TTAA 3 cut(s) 161, 198, 872
MslI CAYNNNNRTG 3 cut(s) 404, 1166, 1481
Msp20I TGGCCA 1 cut(s) 1445
MspA1I CMGCKG 2 cut(s) 314, 1604
MspR9I CCNGG 1 cut(s) 508
Mva1269I GAATGC 1 cut(s) 920
MvaI CCWGG 1 cut(s) 508
MwoI GCNNNNNNNGC 7 cut(s) 108, 230, 864, 1040, 1256, 1387, 1437
NcoI CCATGG 1 cut(s) 1167
NdeII GATC 6 cut(s) 144, 452, 520, 1084, 1228, 1638
NheI GCTAGC 1 cut(s) 332
NlaIV GGNNCC 4 cut(s) 223, 505, 881, 959
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 1 cut(s) 625
NspI RCATGY 2 cut(s) 619, 1486
PagI TCATGA 1 cut(s) 1056
PciI ACATGT 1 cut(s) 1482
PctI GAATGC 1 cut(s) 920
PfeI GAWTC 3 cut(s) 471, 1501, 1583
PflMI CCANNNNNTGG 1 cut(s) 347
PkrI GCNGC 6 cut(s) 19, 313, 323, 1045, 1354, 1606
PleI GAGTC 2 cut(s) 688, 1563
PpsI GAGTC 2 cut(s) 688, 1563
PscI ACATGT 1 cut(s) 1482
Psp6I CCWGG 1 cut(s) 506
PspFI CCCAGC 1 cut(s) 1034
PspGI CCWGG 1 cut(s) 506
PspN4I GGNNCC 4 cut(s) 223, 505, 881, 959
PspPI GGNCC 3 cut(s) 285, 957, 1013
PstNI CAGNNNCTG 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 1604
RseI CAYNNNNRTG 3 cut(s) 404, 1166, 1481
SaqAI TTAA 3 cut(s) 161, 198, 872
SatI GCNGC 6 cut(s) 18, 312, 322, 1044, 1353, 1605
Sau3AI GATC 6 cut(s) 144, 452, 520, 1084, 1228, 1638
Sau96I GGNCC 3 cut(s) 285, 957, 1013
ScaI AGTACT 1 cut(s) 715
SchI GAGTC 2 cut(s) 689, 1563
ScrFI CCNGG 1 cut(s) 508
SexAI ACCWGGT 1 cut(s) 506
SfaNI GCATC 2 cut(s) 573, 1571
SfcI CTRYAG 1 cut(s) 573
SinI GGWCC 1 cut(s) 957
SmiMI CAYNNNNRTG 3 cut(s) 404, 1166, 1481
SmlI CTYRAG 1 cut(s) 21
SmoI CTYRAG 1 cut(s) 21
Sse9I AATT 9 cut(s) 637, 674, 789, 869, 912, 928, 980, 1318, 1366
SsiI CCGC 4 cut(s) 312, 324, 865, 1578
SspMI CTAG 3 cut(s) 333, 1223, 1428
StyD4I CCNGG 1 cut(s) 506
StyI CCWWGG 2 cut(s) 550, 1167
TaaI ACNGT 8 cut(s) 97, 203, 515, 545, 696, 850, 1254, 1271
TaiI ACGT 1 cut(s) 54
TaqI TCGA 2 cut(s) 392, 1680
TasI AATT 9 cut(s) 637, 674, 789, 869, 912, 928, 980, 1318, 1366
TatI WGTACW 5 cut(s) 153, 647, 713, 1404, 1413
TauI GCSGC 1 cut(s) 314
TfiI GAWTC 3 cut(s) 471, 1501, 1583
Tru1I TTAA 3 cut(s) 161, 198, 872
Tru9I TTAA 3 cut(s) 161, 198, 872
TscAI CASTG 4 cut(s) 520, 803, 1006, 1623
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 5 cut(s) 17, 321, 1043, 1352, 1604
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 8 cut(s) 45, 453, 556, 686, 1455, 1643, 1661, 1686
TspRI CASTG 4 cut(s) 520, 803, 1006, 1623
Van91I CCANNNNNTGG 1 cut(s) 347
VpaK11BI GGWCC 1 cut(s) 957
XapI RAATTY 3 cut(s) 637, 980, 1366
XceI RCATGY 2 cut(s) 619, 1486
XspI CTAG 3 cut(s) 333, 1223, 1428
ZrmI AGTACT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.