Rmu_sc0005037.1_g000008

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005037.1
OG0001164
Physical Location & Seq
Reverse (-)
26121 .. 30271
4151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005037.1_g000008.1.cds

Sequence Viewer

Length: 1593 bp
ctgttggattctggtcgctcgtgtttcggtgggcaaagaagtctgcagggttttggagattgtcttgggctgttggtagaccagatctcagaggatgtcgcgggtctttcggaggtggaggaagaccagtggatcagagaggtcgctacgatctttaggggttcgcctattctccagattccgacttggaggggcagcaagttggctgacgttgttcctcgtcggtggatccagacgtgcaggtgctcgatgactttgcttggtttgctgcttgaggtcatcatggccaggctccgttgtggtcggccaccgaagggttggacggcggcagcgagggcttggctactgggggctcgggctactatctttgctgctcgggtttgggcttgggcttttgggcctgagctcaaacttgggacgaggaacgaagaattgatcgagtactttttgaaagaccaaattccgagctggattgctgctgctggctacattgctcttgcaatcatatcaattattgtcctgccattcatgttccaccaactaaaatggtatcacgttgcgatagcttacatcatcgctctggttctagccttctgtaatgcctatggttgcggcctcacagattggtctcttgcttccaaccacggcaaaatcacaattctcatcttcagtgcttggcttggccgtgaaaaaggtggagtcattgctggccttgcttcctgtggtgtaatgatgagcattgtctcaacagcctcggatctcatgcaagacttcaagtccggatacctaactctctcttctcctcgattcatctggttcttctacaaagcttaccgagttggtgatccaaaaggctcatatcctgcaccctatggcataatgtaccgcggtattgcactcctcggagttgaggacatcggctcacaccccaaacactgcgccacgcttgcagtctcattttttgcatttgccattgccattaacatagtaaccgagattttaaagcgttacgagaccaagtacaagatctacagatttgttccgaacgcaatggccatggcagtcccgttctatctcggctcctacttcatgattcacggtgttccagcggcgatcctatctcttgccaatgtcaaagcaccgatatgcatgaagttcctctcttactcggacaacgatggagttgattcatttttatcttcatcaggatctaggagatctctgtgtcattgtctacaaaatattcccgttcttctgatcgataaagaaaaccaagaaacaagattatcatggcgccttgtgttcctgttgttgatcttcgaaggaccgacacaaagagagttggtggctgcgaatgatcatgacgatgatactctgcaatcgcaaaacaaggtgcaaagagtagatgctaatgaaccaaccccaattgctctcgacgatcaaccaaacaatcttgagcagacgaatgacatgccgcctgtgcttgaagaaacccagtttgctgatattgaagagatgaatctcggattgcagcacttactaatggcagcaaatcatgctgatattgaacagaggccagagggtatggcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

530

Amino Acids

58.96

Weight (kDa)

5.98

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 231
Acc36I ACCTGC 1 cut(s) 231
AccB1I GGYRCC 1 cut(s) 1293
AccI GTMKAC 2 cut(s) 78, 1234
AccII CGCG 2 cut(s) 101, 888
AccIII TCCGGA 1 cut(s) 779
AciI CCGC 7 cut(s) 101, 326, 612, 886, 888, 1109, 1475
AclWI GGATC 7 cut(s) 140, 223, 236, 765, 839, 1108, 1216
AcoI YGGCCR 4 cut(s) 285, 305, 682, 1053
AcsI RAATTY 1 cut(s) 459
AcuI CTGAAG 1 cut(s) 652
AcyI GRCGYC 1 cut(s) 1294
AfaI GTAC 3 cut(s) 443, 884, 1022
AfiI CCNNNNNNNGG 1 cut(s) 314
AgsI TTSAA 5 cut(s) 451, 775, 1487, 1511, 1568
AhdI GACNNNNNGTC 1 cut(s) 775
AjiI CACGTC 1 cut(s) 237
AjnI CCWGG 1 cut(s) 287
AluBI AGCT 4 cut(s) 406, 468, 566, 830
AluI AGCT 4 cut(s) 406, 468, 566, 830
Alw21I GWGCWC 2 cut(s) 248, 408
Alw26I GTCTC 4 cut(s) 633, 748, 958, 1007
AlwI GGATC 7 cut(s) 140, 223, 236, 765, 839, 1108, 1216
Ama87I CYCGRG 2 cut(s) 354, 375
Aor13HI TCCGGA 1 cut(s) 779
AoxI GGCC 8 cut(s) 285, 305, 398, 613, 682, 709, 1053, 1574
ApeKI GCWGC 9 cut(s) 195, 268, 329, 371, 476, 479, 1349, 1531, 1547
ApoI RAATTY 1 cut(s) 459
AspLEI GCGC 2 cut(s) 941, 1296
AspS9I GGNCC 2 cut(s) 398, 1325
AsuHPI GGTGA 1 cut(s) 854
AsuII TTCGAA 1 cut(s) 1320
AvaI CYCGRG 2 cut(s) 354, 375
AvaII GGWCC 1 cut(s) 1325
BalI TGGCCA 2 cut(s) 287, 1055
BamHI GGATCC 1 cut(s) 228
BanI GGYRCC 1 cut(s) 1293
BanII GRGCYC 2 cut(s) 355, 408
BauI CACGAG 1 cut(s) 19
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 2 cut(s) 248, 408
BbvI GCAGC 9 cut(s) 207, 255, 341, 358, 463, 466, 1336, 1543, 1559
BccI CCATC 1 cut(s) 1172
BceAI ACGGC 3 cut(s) 339, 661, 669
BcgI CGANNNNNNTGC 4 cut(s) 238, 272, 1453, 1487
BciT130I CCWGG 1 cut(s) 289
BciVI GTATCC 1 cut(s) 776
BclI TGATCA 1 cut(s) 1357
BcoDI GTCTC 4 cut(s) 633, 748, 958, 1007
BfaI CTAG 2 cut(s) 587, 1212
BfmI CTRYAG 2 cut(s) 44, 1030
BfoI RGCGCY 1 cut(s) 1297
BfuAI ACCTGC 1 cut(s) 231
BfuI GTATCC 1 cut(s) 776
BglII AGATCT 3 cut(s) 84, 1026, 1217
BmcAI AGTACT 1 cut(s) 443
Bme1390I CCNGG 1 cut(s) 289
Bme18I GGWCC 1 cut(s) 1325
BmeRI GACNNNNNGTC 1 cut(s) 775
BmeT110I CYCGRG 2 cut(s) 354, 375
BmgBI CACGTC 1 cut(s) 237
BmgT120I GGNCC 2 cut(s) 398, 1325
BmiI GGNNCC 4 cut(s) 230, 293, 1081, 1295
BmrFI CCNGG 1 cut(s) 289
BmrI ACTGGG 2 cut(s) 356, 1489
BmsI GCATC 1 cut(s) 1396
BmuI ACTGGG 2 cut(s) 356, 1489
BpiI GAAGAC 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 158
Bpu10I CCTNAGC 1 cut(s) 402
Bpu14I TTCGAA 1 cut(s) 1320
BpuEI CTTGAG 2 cut(s) 293, 1475
Bsa29I ATCGAT 1 cut(s) 1260
BsaHI GRCGYC 1 cut(s) 1294
BsaI GGTCTC 2 cut(s) 633, 1007
BsaJI CCNNGG 5 cut(s) 643, 753, 886, 901, 1056
BsaWI WCCGGW 1 cut(s) 779
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse1I ACTGG 3 cut(s) 127, 351, 1495
Bse3DI GCAATG 4 cut(s) 489, 702, 972, 1056
BseAI TCCGGA 1 cut(s) 779
BseBI CCWGG 1 cut(s) 289
BseCI ATCGAT 1 cut(s) 1260
BseDI CCNNGG 5 cut(s) 643, 753, 886, 901, 1056
BseGI GGATG 1 cut(s) 100
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMI GCAATG 4 cut(s) 489, 702, 972, 1056
BseMII CTCAG 2 cut(s) 102, 393
BseNI ACTGG 3 cut(s) 127, 351, 1495
BseRI GAGGAG 2 cut(s) 792, 890
BseXI GCAGC 9 cut(s) 207, 255, 341, 358, 463, 466, 1336, 1543, 1559
BsgI GTGCAG 2 cut(s) 259, 849
Bsh1236I CGCG 2 cut(s) 101, 888
BshFI GGCC 8 cut(s) 287, 307, 400, 615, 684, 711, 1055, 1576
BshNI GGYRCC 1 cut(s) 1293
BshVI ATCGAT 1 cut(s) 1260
BsiHKAI GWGCWC 2 cut(s) 248, 408
BsiHKCI CYCGRG 2 cut(s) 354, 375
BsiSI CCGG 1 cut(s) 780
BslFI GGGAC 2 cut(s) 430, 1049
BslI CCNNNNNNNGG 1 cut(s) 314
BsmAI GTCTC 4 cut(s) 633, 748, 958, 1007
BsmFI GGGAC 2 cut(s) 430, 1049
BsnI GGCC 8 cut(s) 287, 307, 400, 615, 684, 711, 1055, 1576
Bso31I GGTCTC 2 cut(s) 633, 1007
BsoBI CYCGRG 2 cut(s) 354, 375
Bsp119I TTCGAA 1 cut(s) 1320
Bsp1286I GDGCHC 3 cut(s) 248, 355, 408
Bsp13I TCCGGA 1 cut(s) 779
Bsp19I CCATGG 1 cut(s) 1056
BspACI CCGC 7 cut(s) 101, 326, 612, 886, 888, 1109, 1475
BspANI GGCC 8 cut(s) 287, 307, 400, 615, 684, 711, 1055, 1576
BspCNI CTCAG 2 cut(s) 101, 394
BspDI ATCGAT 1 cut(s) 1260
BspEI TCCGGA 1 cut(s) 779
BspFNI CGCG 2 cut(s) 101, 888
BspHI TCATGA 2 cut(s) 1089, 1360
BspLI GGNNCC 4 cut(s) 230, 293, 1081, 1295
BspMAI CTGCAG 1 cut(s) 48
BspMI ACCTGC 1 cut(s) 231
BspPI GGATC 7 cut(s) 140, 223, 236, 765, 839, 1108, 1216
BspT104I TTCGAA 1 cut(s) 1320
BspT107I GGYRCC 1 cut(s) 1293
BspTNI GGTCTC 2 cut(s) 633, 1007
BsrDI GCAATG 4 cut(s) 489, 702, 972, 1056
BsrI ACTGG 3 cut(s) 127, 351, 1495
BssECI CCNNGG 5 cut(s) 643, 753, 886, 901, 1056
BssNI GRCGYC 1 cut(s) 1294
BssSI CACGAG 1 cut(s) 19
BssT1I CCWWGG 1 cut(s) 1056
Bst2BI CACGAG 1 cut(s) 19
Bst2UI CCWGG 1 cut(s) 289
Bst4CI ACNGT 1 cut(s) 1100
Bst6I CTCTTC 2 cut(s) 802, 1506
BstACI GRCGYC 1 cut(s) 1294
BstAPI GCANNNNNTGC 1 cut(s) 1556
BstBI TTCGAA 1 cut(s) 1320
BstC8I GCNNGC 3 cut(s) 484, 709, 948
BstDEI CTNAG 2 cut(s) 88, 402
BstDSI CCRYGG 3 cut(s) 643, 886, 1056
BstF5I GGATG 1 cut(s) 100
BstFNI CGCG 2 cut(s) 101, 888
BstH2I RGCGCY 1 cut(s) 1297
BstHHI GCGC 2 cut(s) 941, 1296
BstMAI GTCTC 4 cut(s) 633, 748, 958, 1007
BstMWI GCNNNNNNNGC 6 cut(s) 265, 335, 713, 947, 1480, 1556
BstNI CCWGG 1 cut(s) 289
BstNSI RCATGY 1 cut(s) 1474
BstSCI CCNGG 1 cut(s) 287
BstSFI CTRYAG 2 cut(s) 44, 1030
BstUI CGCG 2 cut(s) 101, 888
BstV1I GCAGC 9 cut(s) 207, 255, 341, 358, 463, 466, 1336, 1543, 1559
BstV2I GAAGAC 1 cut(s) 129
BstX2I RGATCY 6 cut(s) 84, 228, 757, 1026, 1208, 1217
BstYI RGATCY 6 cut(s) 84, 228, 757, 1026, 1208, 1217
Bsu15I ATCGAT 1 cut(s) 1260
BsuI GTATCC 1 cut(s) 776
BsuRI GGCC 8 cut(s) 287, 307, 400, 615, 684, 711, 1055, 1576
BsuTUI ATCGAT 1 cut(s) 1260
BtgI CCRYGG 3 cut(s) 643, 886, 1056
BtgZI GCGATG 1 cut(s) 559
BtrI CACGTC 1 cut(s) 237
BtsCI GGATG 1 cut(s) 100
BtsI GCAGTG 1 cut(s) 934
BtsIMutI CAGTG 3 cut(s) 134, 676, 934
BveI ACCTGC 1 cut(s) 231
Cac8I GCNNGC 3 cut(s) 484, 709, 948
CciI TCATGA 2 cut(s) 1089, 1360
CfoI GCGC 2 cut(s) 941, 1296
Cfr13I GGNCC 2 cut(s) 398, 1325
Cfr42I CCGCGG 1 cut(s) 889
ClaI ATCGAT 1 cut(s) 1260
Csp6I GTAC 3 cut(s) 442, 883, 1021
CviQI GTAC 3 cut(s) 442, 883, 1021
DdeI CTNAG 2 cut(s) 88, 402
DinI GGCGCC 1 cut(s) 1295
DraI TTTAAA 1 cut(s) 1002
DriI GACNNNNNGTC 1 cut(s) 775
EaeI YGGCCR 4 cut(s) 285, 305, 682, 1053
Eam1104I CTCTTC 2 cut(s) 802, 1506
Eam1105I GACNNNNNGTC 1 cut(s) 775
EarI CTCTTC 2 cut(s) 802, 1506
Ecl136II GAGCTC 1 cut(s) 406
Eco130I CCWWGG 1 cut(s) 1056
Eco24I GRGCYC 2 cut(s) 355, 408
Eco31I GGTCTC 2 cut(s) 633, 1007
Eco47I GGWCC 1 cut(s) 1325
Eco53kI GAGCTC 1 cut(s) 406
Eco57I CTGAAG 1 cut(s) 652
Eco88I CYCGRG 2 cut(s) 354, 375
EcoICRI GAGCTC 1 cut(s) 406
EcoRII CCWGG 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 1056
EcoT22I ATGCAT 1 cut(s) 1151
EcoT38I GRGCYC 2 cut(s) 355, 408
EgeI GGCGCC 1 cut(s) 1295
EheI GGCGCC 1 cut(s) 1295
ErhI CCWWGG 1 cut(s) 1056
FaqI GGGAC 2 cut(s) 430, 1049
FauI CCCGC 1 cut(s) 94
FbaI TGATCA 1 cut(s) 1357
FblI GTMKAC 2 cut(s) 78, 1234
FokI GGATG 1 cut(s) 107
FriOI GRGCYC 2 cut(s) 355, 408
FspBI CTAG 2 cut(s) 587, 1212
GlaI GCGC 2 cut(s) 940, 1295
GsuI CTGGAG 1 cut(s) 158
HaeII RGCGCY 1 cut(s) 1297
HaeIII GGCC 8 cut(s) 287, 307, 400, 615, 684, 711, 1055, 1576
HapII CCGG 1 cut(s) 780
HhaI GCGC 2 cut(s) 941, 1296
Hin1I GRCGYC 1 cut(s) 1294
Hin6I GCGC 2 cut(s) 939, 1294
HinP1I GCGC 2 cut(s) 939, 1294
HindIII AAGCTT 1 cut(s) 828
HinfI GANTC 7 cut(s) 8, 178, 699, 807, 1093, 1187, 1519
HpaII CCGG 1 cut(s) 780
HphI GGTGA 1 cut(s) 854
Hpy166II GTNNAC 2 cut(s) 79, 1235
Hpy188III TCNNGA 8 cut(s) 175, 232, 780, 1090, 1206, 1361, 1433, 1454
Hpy8I GTNNAC 2 cut(s) 79, 1235
Hpy99I CGWCG 2 cut(s) 225, 1439
HpyAV CCTTC 3 cut(s) 307, 601, 1316
HpyCH4III ACNGT 1 cut(s) 1100
HpyCH4IV ACGT 3 cut(s) 210, 236, 555
HpyF10VI GCNNNNNNNGC 6 cut(s) 265, 335, 713, 947, 1480, 1556
HpyF3I CTNAG 2 cut(s) 88, 402
HpySE526I ACGT 3 cut(s) 210, 236, 555
Hsp92I GRCGYC 1 cut(s) 1294
HspAI GCGC 2 cut(s) 939, 1294
KasI GGCGCC 1 cut(s) 1293
Kpn2I TCCGGA 1 cut(s) 779
Ksp22I TGATCA 1 cut(s) 1357
KspI CCGCGG 1 cut(s) 889
LmnI GCTCC 2 cut(s) 297, 1085
Lsp1109I GCAGC 9 cut(s) 207, 255, 341, 358, 463, 466, 1336, 1543, 1559
LweI GCATC 1 cut(s) 1396
MaeI CTAG 2 cut(s) 587, 1212
MaeII ACGT 3 cut(s) 210, 236, 555
MaeIII GTNAC 2 cut(s) 988, 1007
MfeI CAATTG 1 cut(s) 1425
MflI RGATCY 6 cut(s) 84, 228, 757, 1026, 1208, 1217
MhlI GDGCHC 3 cut(s) 248, 355, 408
MlsI TGGCCA 2 cut(s) 287, 1055
MluCI AATT 5 cut(s) 431, 459, 510, 657, 1425
MluNI TGGCCA 2 cut(s) 287, 1055
Mly113I GGCGCC 1 cut(s) 1294
MlyI GAGTC 1 cut(s) 708
MmeI TCCRAC 3 cut(s) 206, 299, 663
Mox20I TGGCCA 2 cut(s) 287, 1055
Mph1103I ATGCAT 1 cut(s) 1151
MroI TCCGGA 1 cut(s) 779
MscI TGGCCA 2 cut(s) 287, 1055
MseI TTAA 3 cut(s) 981, 1001, 1591
MslI CAYNNNNRTG 2 cut(s) 1144, 1365
Msp20I TGGCCA 2 cut(s) 287, 1055
MspA1I CMGCKG 2 cut(s) 888, 1109
MspI CCGG 1 cut(s) 780
MspR9I CCNGG 1 cut(s) 289
MunI CAATTG 1 cut(s) 1425
MvaI CCWGG 1 cut(s) 289
MvnI CGCG 2 cut(s) 101, 888
MwoI GCNNNNNNNGC 6 cut(s) 265, 335, 713, 947, 1480, 1556
NarI GGCGCC 1 cut(s) 1294
NcoI CCATGG 1 cut(s) 1056
NlaIV GGNNCC 4 cut(s) 230, 293, 1081, 1295
NmeAIII GCCGAG 1 cut(s) 1056
NsiI ATGCAT 1 cut(s) 1151
NspI RCATGY 1 cut(s) 1474
NspV TTCGAA 1 cut(s) 1320
PagI TCATGA 2 cut(s) 1089, 1360
PaqCI CACCTGC 1 cut(s) 231
PcsI WCGNNNNNNNCGW 1 cut(s) 329
PfeI GAWTC 6 cut(s) 8, 178, 807, 1093, 1187, 1519
PleI GAGTC 1 cut(s) 707
PluTI GGCGCC 1 cut(s) 1297
PpsI GAGTC 1 cut(s) 707
Psp124BI GAGCTC 1 cut(s) 408
Psp6I CCWGG 1 cut(s) 287
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 4 cut(s) 230, 293, 1081, 1295
PspPI GGNCC 2 cut(s) 398, 1325
PstI CTGCAG 1 cut(s) 48
PsuI RGATCY 6 cut(s) 84, 228, 757, 1026, 1208, 1217
RsaI GTAC 3 cut(s) 443, 884, 1022
RsaNI GTAC 3 cut(s) 442, 883, 1021
RseI CAYNNNNRTG 2 cut(s) 1144, 1365
SacI GAGCTC 1 cut(s) 408
SacII CCGCGG 1 cut(s) 889
SaqAI TTAA 3 cut(s) 981, 1001, 1591
Sau96I GGNCC 2 cut(s) 398, 1325
ScaI AGTACT 1 cut(s) 443
SchI GAGTC 1 cut(s) 708
ScrFI CCNGG 1 cut(s) 289
SduI GDGCHC 3 cut(s) 248, 355, 408
SfaNI GCATC 1 cut(s) 1396
SfcI CTRYAG 2 cut(s) 44, 1030
SfoI GGCGCC 1 cut(s) 1295
Sfr303I CCGCGG 1 cut(s) 889
SfuI TTCGAA 1 cut(s) 1320
SgrBI CCGCGG 1 cut(s) 889
SinI GGWCC 1 cut(s) 1325
SmiMI CAYNNNNRTG 2 cut(s) 1144, 1365
SmlI CTYRAG 2 cut(s) 272, 1454
SmoI CTYRAG 2 cut(s) 272, 1454
Sse9I AATT 5 cut(s) 431, 459, 510, 657, 1425
SsiI CCGC 7 cut(s) 101, 326, 612, 886, 888, 1109, 1475
SspDI GGCGCC 1 cut(s) 1293
SspI AATATT 1 cut(s) 1243
SspMI CTAG 2 cut(s) 587, 1212
SstI GAGCTC 1 cut(s) 408
StyD4I CCNGG 1 cut(s) 287
StyI CCWWGG 1 cut(s) 1056
TaaI ACNGT 1 cut(s) 1100
TaiI ACGT 3 cut(s) 213, 239, 558
TaqI TCGA 6 cut(s) 248, 438, 805, 1260, 1320, 1434
TaqII GACCGA 1 cut(s) 1342
TasI AATT 5 cut(s) 431, 459, 510, 657, 1425
TatI WGTACW 2 cut(s) 441, 1020
TauI GCSGC 4 cut(s) 329, 615, 1112, 1477
TfiI GAWTC 6 cut(s) 8, 178, 807, 1093, 1187, 1519
Tru1I TTAA 3 cut(s) 981, 1001, 1591
Tru9I TTAA 3 cut(s) 981, 1001, 1591
TscAI CASTG 3 cut(s) 134, 676, 941
TseI GCWGC 9 cut(s) 195, 268, 329, 371, 476, 479, 1349, 1531, 1547
TspDTI ATGAA 8 cut(s) 517, 799, 1078, 1166, 1179, 1191, 1428, 1532
TspGWI ACGGA 1 cut(s) 284
TspRI CASTG 3 cut(s) 134, 676, 941
VpaK11BI GGWCC 1 cut(s) 1325
XapI RAATTY 1 cut(s) 459
XceI RCATGY 1 cut(s) 1474
XcmI CCANNNNNNNNNTGG 1 cut(s) 315
XmiI GTMKAC 2 cut(s) 78, 1234
XspI CTAG 2 cut(s) 587, 1212
ZrmI AGTACT 1 cut(s) 443
Zsp2I ATGCAT 1 cut(s) 1151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.