MD16G1001100.v1.1

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001164
Physical Location & Seq
Reverse (-)
63429 .. 66701
3273 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1001100.v1.1.491

Sequence Viewer

Length: 2118 bp
ATGGAGAGAAGCCTTAGCAAGGGCAGTGATAACCAGAGTGACGACTTGGATGCCAAGAAAGGCACTGCCGGAAAGACCAAAAAGAAGTTGAATGTTGAGGAGGCGTTCAAGGCCACGGCGGTGCCGCCGTGGACCAAGCAGATTACGGTGAGGTCGATGGTCACCAGCTTCATTCTCAGCATCGTCTTCAACTTCATCGTCTGCAAGTTGAACCTCACCACTGGTGTTATTCCCTCGCTCAACGTCGCCGCCGGACTGCTCGGCTTCGCCGTGGTGAAAGGCTACACCGCCATGATCGACAAGTGCGGCTTCTTGAAGCAGCCTTTCACTCGCCAGGAAAACACCGTCATTCAGACTTGCGTCGTCGCCTCTTCCGGGATCGCCTTTAGCAGTGGAACTGCAAGTTATTTACTGGGAATGAGTGCAAAGATTGCTGCGCAAGGAGATCTAGGCAACACCCCGATCAACATAAAGAAGCTTGATGTTGGATGGATGATTGGGTTTCTCTTCGCTGTCAGCTTCGTCGGGTTGTTTTCGATTATGCCCCTCAGGAAGATGATGATCCTGAAGTACAAGTTAACGTACCCGAGTGGAACTGCAACTGCATACCTCATCAACAGCTTTCACACACCCAAAGGAGCTAAGCTGGCAAAGAAACAGGTTGCTGTGCTCTTCAAAAGCTTCTGTTTCAGCTTTGCGTTTGCCTTCTTCCAGTGGTTTTTCGCTGCTGCTGACGGCTGCGGATTTTCCAGCTTCCCAACATTTGGTCTTAAAGCCTTTGCTAACAAGTTTTACTTTGATTTTTCAGCGACTTATGTTGGCGTTGGCATGATCTGCCCTATCATGGTCAATGTATCTTTGCTTGTTGGAGCCATTATTTCATGGGGAATCATGTGGCCCTTGATTGAGACAAAGAAAGGTATCTGGTACAGCGCTAATCTATCCGCCAGCAGTCTCCACGGCATCCAAGGATACAGGGTTTTTATTGCTATAGCGATGATGCTTGGCGACGGTTTGTTCCATGTAGTCTACATGCTCTTCATGACCACAAAAAGTCTCGCGACACAAAGATCAGAGAAGAAACTTGAGTCGTCTTCATCACCACCTGTAAATCTTGACAGTGTTGAAGCCGGAGGGTCCTCCGAAGTTGTCTCGGCAAACTATGATGAACAACGAAGAATCGAGTACTTCTTGAAAGACCAAATCCCCAGCTGGGTTGCTTTCTCTGGCTACATTGTGCTCGCAGCCATATCCATCAGTGTGGTACCCTTGATCTTCCCCCAACTGAAATGGTACCATGTGCTGGTTGCATATCTGATTGCCCCAGTCTTGGCCTTCTGCAATGCCTACGGCTGTGGCCTCACTGATTGGTCTCTTGCCTCCAACTATGGCAAATTTGCCATCATAATCTTCAGCGCTTGGGTTGGCCTTGACCACGGTGGTGTCATTGCCGGCCTTGCTTCTTGTGGTGTGATGATGAGCATTGTCGCGACCGCTTCTGATCTCATGCAAGACTTCAAGACAGGATACCTCACGCTCTCGTCTCCTCGATCCATGTTCTTCAGCCAAGTCTTTGGCACAGCCATGGGATGCGTCATGTCTCCATTAGTATTCTGGGTTTTCTACAAAGCTTACCCACTTGGTGATCCAGACGGCCCTTATCCTGCACCCTACGGCCTAATGTACCGCGGCATTGCACTCCTTGGAGTTGAGGGTGTCGGTTCCCTCCCCAAAAACTGTGTCGTGCTTGCAGTCTCGTTTTTCGCAGCTGCAGTCGCCATAAACATAATCATCGAGCTGTTACAGCGTTATGAAACCAAGTACAGGATCTATAGGTTCATTCCCAGCCCAATGTGCATGGCGATTCCATTCTACCTGGGTTCCTACTTCGCCATTGACATGTGCGTGGGAAGCTTGATCCTCGTCCTGTGGCGGAGGAAGAACAGGCAAAAGGCCGATGACTTTGGGCCAGCTGTGGCGTCGGGACTCATCTGCGGTGATTCATTGTGGGGCGTTCCGGCTGCTATCCTGGCCATTGCGTCTGTCAAAGCTCCTATCTGCATGAAGTTCCTCTCTGCTTCTGCCAACAACAAAGTTGATTCCCTATTAGGTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

706

Amino Acids

76.53

Weight (kDa)

9.24

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 47 - 677 2.5e-158 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 438
Acc65I GGTACC 2 cut(s) 1264, 1293
AccB1I GGYRCC 3 cut(s) 121, 1264, 1293
AccB7I CCANNNNNTGG 2 cut(s) 764, 1303
AccI GTMKAC 1 cut(s) 1029
AccII CGCG 3 cut(s) 1061, 1490, 1689
AclWI GGATC 6 cut(s) 386, 556, 1545, 1640, 1835, 1912
AcoI YGGCCR 1 cut(s) 2031
AcsI RAATTY 1 cut(s) 1394
AcuI CTGAAG 3 cut(s) 587, 1396, 1546
AcyI GRCGYC 1 cut(s) 1979
AdeI CACNNNGTG 1 cut(s) 1643
AfaI GTAC 8 cut(s) 572, 584, 929, 1187, 1266, 1295, 1685, 1823
AfeI AGCGCT 2 cut(s) 934, 1417
AfiI CCNNNNNNNGG 8 cut(s) 19, 375, 764, 844, 1213, 1303, 1330, 1824
AflIII ACRYGT 1 cut(s) 1899
AgsI TTSAA 9 cut(s) 91, 109, 190, 211, 316, 676, 1127, 1195, 1519
AjnI CCWGG 3 cut(s) 333, 1875, 2028
AleI CACNNNNGTG 2 cut(s) 119, 1440
Alw21I GWGCWC 2 cut(s) 672, 1242
Alw26I GTCTC 8 cut(s) 902, 959, 1061, 1156, 1377, 1548, 1605, 1759
AlwI GGATC 6 cut(s) 386, 556, 1545, 1640, 1835, 1912
Ama87I CYCGRG 1 cut(s) 586
Aor51HI AGCGCT 2 cut(s) 934, 1417
ApeKI GCWGC 9 cut(s) 319, 434, 725, 728, 738, 1244, 1766, 1769, 2021
ApoI RAATTY 1 cut(s) 1394
ArsI GACNNNNNNTTYG 2 cut(s) 1725, 1757
Asp718I GGTACC 2 cut(s) 1264, 1293
AspLEI GCGC 3 cut(s) 439, 935, 1418
AspS9I GGNCC 5 cut(s) 132, 897, 1137, 1655, 1967
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 7 cut(s) 154, 160, 208, 286, 1092, 1655, 2009
AvaI CYCGRG 1 cut(s) 586
AvaII GGWCC 2 cut(s) 132, 1137
AxyI CCTNAGG 1 cut(s) 548
BalI TGGCCA 1 cut(s) 2033
BanI GGYRCC 3 cut(s) 121, 1264, 1293
BbsI GAAGAC 2 cut(s) 178, 1086
Bbv12I GWGCWC 2 cut(s) 672, 1242
BbvI GCAGC 9 cut(s) 331, 421, 712, 715, 725, 1256, 1756, 1778, 2008
BccI CCATC 4 cut(s) 151, 483, 1262, 1409
BceAI ACGGC 8 cut(s) 112, 132, 254, 751, 976, 1366, 1669, 1690
BcgI CGANNNNNNTGC 2 cut(s) 32, 66
BciT130I CCWGG 3 cut(s) 335, 1877, 2030
BciVI GTATCC 2 cut(s) 965, 1520
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 8 cut(s) 902, 959, 1061, 1156, 1377, 1548, 1605, 1759
BfaI CTAG 2 cut(s) 449, 2116
BfmI CTRYAG 3 cut(s) 990, 1770, 1831
BfoI RGCGCY 2 cut(s) 936, 1419
BfuI GTATCC 2 cut(s) 965, 1520
BglII AGATCT 1 cut(s) 445
BlpI GCTNAGC 1 cut(s) 642
BmcAI AGTACT 1 cut(s) 1187
Bme1390I CCNGG 4 cut(s) 335, 376, 1877, 2030
Bme18I GGWCC 2 cut(s) 132, 1137
BmeT110I CYCGRG 1 cut(s) 586
BmgT120I GGNCC 5 cut(s) 132, 897, 1137, 1655, 1967
BmiI GGNNCC 7 cut(s) 123, 871, 1138, 1266, 1295, 1723, 1882
BmrFI CCNGG 4 cut(s) 335, 376, 1877, 2030
BmrI ACTGGG 2 cut(s) 422, 1319
BmsI GCATC 5 cut(s) 40, 189, 972, 990, 1580
BmuI ACTGGG 2 cut(s) 422, 1319
BoxI GACNNNNGTC 1 cut(s) 359
BpiI GAAGAC 2 cut(s) 178, 1086
Bpu10I CCTNAGC 1 cut(s) 14
Bpu1102I GCTNAGC 1 cut(s) 642
BpuEI CTTGAG 1 cut(s) 1106
BpuMI CCSGG 1 cut(s) 376
BsaBI GATNNNNATC 1 cut(s) 560
BsaHI GRCGYC 1 cut(s) 1979
BsaI GGTCTC 1 cut(s) 1377
Bsc4I CCNNNNNNNGG 8 cut(s) 19, 375, 764, 844, 1213, 1303, 1330, 1824
Bse118I RCCGGY 1 cut(s) 1451
Bse1I ACTGG 4 cut(s) 226, 417, 712, 1325
Bse21I CCTNAGG 1 cut(s) 548
Bse3DI GCAATG 4 cut(s) 1348, 1446, 1692, 2034
Bse8I GATNNNNATC 1 cut(s) 560
BseBI CCWGG 3 cut(s) 335, 1877, 2030
BseGI GGATG 5 cut(s) 55, 494, 498, 963, 1595
BseJI GATNNNNATC 1 cut(s) 560
BseLI CCNNNNNNNGG 8 cut(s) 19, 375, 764, 844, 1213, 1303, 1330, 1824
BseMI GCAATG 4 cut(s) 1348, 1446, 1692, 2034
BseMII CTCAG 2 cut(s) 190, 562
BseNI ACTGG 4 cut(s) 226, 417, 712, 1325
BseRI GAGGAG 2 cut(s) 113, 1536
BseXI GCAGC 9 cut(s) 331, 421, 712, 715, 725, 1256, 1756, 1778, 2008
BseYI CCCAGC 3 cut(s) 1208, 1212, 1844
BsgI GTGCAG 1 cut(s) 1650
Bsh1236I CGCG 3 cut(s) 1061, 1490, 1689
Bsh1285I CGRYCG 1 cut(s) 1494
BshNI GGYRCC 3 cut(s) 121, 1264, 1293
BsiEI CGRYCG 1 cut(s) 1494
BsiHKAI GWGCWC 2 cut(s) 672, 1242
BsiHKCI CYCGRG 1 cut(s) 586
BsiSI CCGG 6 cut(s) 69, 252, 375, 1131, 1452, 2018
BslFI GGGAC 1 cut(s) 1998
BslI CCNNNNNNNGG 8 cut(s) 19, 375, 764, 844, 1213, 1303, 1330, 1824
BsmAI GTCTC 8 cut(s) 902, 959, 1061, 1156, 1377, 1548, 1605, 1759
BsmBI CGTCTC 1 cut(s) 1548
BsmFI GGGAC 1 cut(s) 1998
Bso31I GGTCTC 1 cut(s) 1377
BsoBI CYCGRG 1 cut(s) 586
Bsp1286I GDGCHC 2 cut(s) 672, 1242
Bsp1720I GCTNAGC 1 cut(s) 642
Bsp19I CCATGG 1 cut(s) 1584
Bsp68I TCGCGA 2 cut(s) 1061, 1490
BspCNI CTCAG 2 cut(s) 189, 561
BspFNI CGCG 3 cut(s) 1061, 1490, 1689
BspHI TCATGA 1 cut(s) 1041
BspLI GGNNCC 7 cut(s) 123, 871, 1138, 1266, 1295, 1723, 1882
BspMAI CTGCAG 1 cut(s) 1774
BspPI GGATC 6 cut(s) 386, 556, 1545, 1640, 1835, 1912
BspQI GCTCTTC 2 cut(s) 677, 1043
BspT107I GGYRCC 3 cut(s) 121, 1264, 1293
BspTNI GGTCTC 1 cut(s) 1377
BsrDI GCAATG 4 cut(s) 1348, 1446, 1692, 2034
BsrFI RCCGGY 1 cut(s) 1451
BsrI ACTGG 4 cut(s) 226, 417, 712, 1325
BssAI RCCGGY 1 cut(s) 1451
BssNI GRCGYC 1 cut(s) 1979
BssT1I CCWWGG 3 cut(s) 967, 1584, 1702
Bst2UI CCWGG 3 cut(s) 335, 1877, 2030
Bst4CI ACNGT 6 cut(s) 148, 346, 1013, 1121, 1439, 1739
Bst6I CTCTTC 4 cut(s) 376, 512, 677, 1043
BstACI GRCGYC 1 cut(s) 1979
BstAPI GCANNNNNTGC 2 cut(s) 431, 834
BstC8I GCNNGC 6 cut(s) 648, 949, 1242, 1453, 1749, 1971
BstDEI CTNAG 4 cut(s) 14, 176, 548, 642
BstDSI CCRYGG 7 cut(s) 114, 128, 270, 958, 1435, 1584, 1687
BstEII GGTNACC 1 cut(s) 160
BstENI CCTNNNNNAGG 1 cut(s) 17
BstF5I GGATG 5 cut(s) 55, 494, 498, 963, 1595
BstFNI CGCG 3 cut(s) 1061, 1490, 1689
BstH2I RGCGCY 2 cut(s) 936, 1419
BstHHI GCGC 3 cut(s) 439, 935, 1418
BstMAI GTCTC 8 cut(s) 902, 959, 1061, 1156, 1377, 1548, 1605, 1759
BstMCI CGRYCG 1 cut(s) 1494
BstNI CCWGG 3 cut(s) 335, 1877, 2030
BstNSI RCATGY 2 cut(s) 1036, 1903
BstPAI GACNNNNGTC 1 cut(s) 359
BstPI GGTNACC 1 cut(s) 160
BstSCI CCNGG 4 cut(s) 333, 374, 1875, 2028
BstSFI CTRYAG 3 cut(s) 990, 1770, 1831
BstUI CGCG 3 cut(s) 1061, 1490, 1689
BstV1I GCAGC 9 cut(s) 331, 421, 712, 715, 725, 1256, 1756, 1778, 2008
BstV2I GAAGAC 2 cut(s) 178, 1086
BstX2I RGATCY 2 cut(s) 445, 1827
BstXI CCANNNNNNTGG 2 cut(s) 1261, 1574
BstYI RGATCY 2 cut(s) 445, 1827
Bsu36I CCTNAGG 1 cut(s) 548
BsuI GTATCC 2 cut(s) 965, 1520
BtgI CCRYGG 7 cut(s) 114, 128, 270, 958, 1435, 1584, 1687
BtgZI GCGATG 1 cut(s) 1010
BtsCI GGATG 5 cut(s) 55, 494, 498, 963, 1595
BtsI GCAGTG 3 cut(s) 31, 63, 397
BtsIMutI CAGTG 8 cut(s) 31, 63, 219, 397, 719, 1126, 1264, 1362
BtuMI TCGCGA 2 cut(s) 1061, 1490
Cac8I GCNNGC 6 cut(s) 648, 949, 1242, 1453, 1749, 1971
CciI TCATGA 1 cut(s) 1041
CfoI GCGC 3 cut(s) 439, 935, 1418
Cfr10I RCCGGY 1 cut(s) 1451
Cfr13I GGNCC 5 cut(s) 132, 897, 1137, 1655, 1967
Cfr42I CCGCGG 1 cut(s) 1690
CseI GACGC 4 cut(s) 349, 1582, 1968, 2028
Csp6I GTAC 8 cut(s) 571, 583, 928, 1186, 1265, 1294, 1684, 1822
CviQI GTAC 8 cut(s) 571, 583, 928, 1186, 1265, 1294, 1684, 1822
DdeI CTNAG 4 cut(s) 14, 176, 548, 642
DraIII CACNNNGTG 1 cut(s) 1643
EaeI YGGCCR 1 cut(s) 2031
Eam1104I CTCTTC 4 cut(s) 376, 512, 677, 1043
EarI CTCTTC 4 cut(s) 376, 512, 677, 1043
EciI GGCGGA 2 cut(s) 934, 1948
Eco130I CCWWGG 3 cut(s) 967, 1584, 1702
Eco31I GGTCTC 1 cut(s) 1377
Eco47I GGWCC 2 cut(s) 132, 1137
Eco47III AGCGCT 2 cut(s) 934, 1417
Eco57I CTGAAG 3 cut(s) 587, 1396, 1546
Eco81I CCTNAGG 1 cut(s) 548
Eco88I CYCGRG 1 cut(s) 586
Eco91I GGTNACC 1 cut(s) 160
EcoNI CCTNNNNNAGG 1 cut(s) 17
EcoO109I RGGNCCY 1 cut(s) 1137
EcoO65I GGTNACC 1 cut(s) 160
EcoRII CCWGG 3 cut(s) 333, 1875, 2028
EcoT14I CCWWGG 3 cut(s) 967, 1584, 1702
ErhI CCWWGG 3 cut(s) 967, 1584, 1702
Esp3I CGTCTC 1 cut(s) 1548
FalI AAGNNNNNCTT 4 cut(s) 293, 325, 779, 811
FaqI GGGAC 1 cut(s) 1998
FblI GTMKAC 1 cut(s) 1029
FokI GGATG 5 cut(s) 62, 501, 505, 950, 1602
FspBI CTAG 2 cut(s) 449, 2116
FspI TGCGCA 1 cut(s) 438
GlaI GCGC 3 cut(s) 438, 934, 1417
GsaI CCCAGC 3 cut(s) 1212, 1216, 1848
HaeII RGCGCY 2 cut(s) 936, 1419
HapII CCGG 6 cut(s) 69, 252, 375, 1131, 1452, 2018
HgaI GACGC 4 cut(s) 349, 1582, 1968, 2028
HhaI GCGC 3 cut(s) 439, 935, 1418
Hin1I GRCGYC 1 cut(s) 1979
Hin6I GCGC 3 cut(s) 437, 933, 1416
HinP1I GCGC 3 cut(s) 437, 933, 1416
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HindIII AAGCTT 4 cut(s) 476, 679, 1629, 1912
HinfI GANTC 7 cut(s) 888, 1088, 1179, 1864, 1986, 2000, 2099
HpaI GTTAAC 1 cut(s) 579
HpaII CCGG 6 cut(s) 69, 252, 375, 1131, 1452, 2018
HphI GGTGA 7 cut(s) 154, 160, 208, 286, 1092, 1655, 2009
Hpy166II GTNNAC 3 cut(s) 132, 579, 1030
Hpy188I TCNGA 5 cut(s) 354, 1075, 1144, 1317, 1501
Hpy8I GTNNAC 3 cut(s) 132, 579, 1030
Hpy99I CGWCG 6 cut(s) 248, 365, 368, 527, 1013, 1984
HpyAV CCTTC 2 cut(s) 715, 1345
HpyCH4III ACNGT 6 cut(s) 148, 346, 1013, 1121, 1439, 1739
HpyCH4IV ACGT 2 cut(s) 243, 581
HpyF3I CTNAG 4 cut(s) 14, 176, 548, 642
HpySE526I ACGT 2 cut(s) 243, 581
Hsp92I GRCGYC 1 cut(s) 1979
HspAI GCGC 3 cut(s) 437, 933, 1416
KpnI GGTACC 2 cut(s) 1268, 1297
KroI GCCGGC 1 cut(s) 1451
KroNI GCCGGC 1 cut(s) 1453
KspAI GTTAAC 1 cut(s) 579
KspI CCGCGG 1 cut(s) 1690
LguI GCTCTTC 2 cut(s) 677, 1043
LmnI GCTCC 3 cut(s) 638, 869, 2056
Lsp1109I GCAGC 9 cut(s) 331, 421, 712, 715, 725, 1256, 1756, 1778, 2008
LweI GCATC 5 cut(s) 40, 189, 972, 990, 1580
MaeI CTAG 2 cut(s) 449, 2116
MaeII ACGT 2 cut(s) 243, 581
MaeIII GTNAC 3 cut(s) 38, 160, 1800
MflI RGATCY 2 cut(s) 445, 1827
MhlI GDGCHC 2 cut(s) 672, 1242
MlsI TGGCCA 1 cut(s) 2033
MluCI AATT 1 cut(s) 1394
MluNI TGGCCA 1 cut(s) 2033
MlyI GAGTC 2 cut(s) 1097, 1980
MmeI TCCRAC 3 cut(s) 466, 847, 1407
Mox20I TGGCCA 1 cut(s) 2033
MroNI GCCGGC 1 cut(s) 1451
MscI TGGCCA 1 cut(s) 2033
MseI TTAA 2 cut(s) 578, 771
MslI CAYNNNNRTG 7 cut(s) 119, 290, 1259, 1440, 1583, 1898, 1904
Msp20I TGGCCA 1 cut(s) 2033
MspA1I CMGCKG 4 cut(s) 1212, 1689, 1769, 1973
MspI CCGG 6 cut(s) 69, 252, 375, 1131, 1452, 2018
MspR9I CCNGG 4 cut(s) 335, 376, 1877, 2030
MvaI CCWGG 3 cut(s) 335, 1877, 2030
MvnI CGCG 3 cut(s) 1061, 1490, 1689
NaeI GCCGGC 1 cut(s) 1453
NciI CCSGG 1 cut(s) 376
NcoI CCATGG 1 cut(s) 1584
NgoMIV GCCGGC 1 cut(s) 1451
NlaIV GGNNCC 7 cut(s) 123, 871, 1138, 1266, 1295, 1723, 1882
NmeAIII GCCGAG 2 cut(s) 240, 1133
NmuCI GTSAC 2 cut(s) 38, 160
NruI TCGCGA 2 cut(s) 1061, 1490
NsbI TGCGCA 1 cut(s) 438
NspI RCATGY 2 cut(s) 1036, 1903
OliI CACNNNNGTG 2 cut(s) 119, 1440
PagI TCATGA 1 cut(s) 1041
PciI ACATGT 1 cut(s) 1899
PciSI GCTCTTC 2 cut(s) 677, 1043
PcsI WCGNNNNNNNCGW 2 cut(s) 152, 267
PdiI GCCGGC 1 cut(s) 1453
PfeI GAWTC 5 cut(s) 888, 1179, 1864, 2000, 2099
PflMI CCANNNNNTGG 2 cut(s) 764, 1303
PfoI TCCNGGA 1 cut(s) 374
PleI GAGTC 2 cut(s) 1096, 1980
PpsI GAGTC 2 cut(s) 1096, 1980
PpuMI RGGWCCY 1 cut(s) 1137
PscI ACATGT 1 cut(s) 1899
PshAI GACNNNNGTC 1 cut(s) 359
Psp5II RGGWCCY 1 cut(s) 1137
Psp6I CCWGG 3 cut(s) 333, 1875, 2028
PspEI GGTNACC 1 cut(s) 160
PspFI CCCAGC 3 cut(s) 1208, 1212, 1844
PspGI CCWGG 3 cut(s) 333, 1875, 2028
PspN4I GGNNCC 7 cut(s) 123, 871, 1138, 1266, 1295, 1723, 1882
PspPI GGNCC 5 cut(s) 132, 897, 1137, 1655, 1967
PspPPI RGGWCCY 1 cut(s) 1137
PstI CTGCAG 1 cut(s) 1774
PsuI RGATCY 2 cut(s) 445, 1827
PvuII CAGCTG 3 cut(s) 1212, 1769, 1973
RruI TCGCGA 2 cut(s) 1061, 1490
RsaI GTAC 8 cut(s) 572, 584, 929, 1187, 1266, 1295, 1685, 1823
RsaNI GTAC 8 cut(s) 571, 583, 928, 1186, 1265, 1294, 1684, 1822
RseI CAYNNNNRTG 7 cut(s) 119, 290, 1259, 1440, 1583, 1898, 1904
SacII CCGCGG 1 cut(s) 1690
SapI GCTCTTC 2 cut(s) 677, 1043
SaqAI TTAA 2 cut(s) 578, 771
Sau96I GGNCC 5 cut(s) 132, 897, 1137, 1655, 1967
ScaI AGTACT 1 cut(s) 1187
SchI GAGTC 2 cut(s) 1097, 1980
ScrFI CCNGG 4 cut(s) 335, 376, 1877, 2030
SduI GDGCHC 2 cut(s) 672, 1242
SfaNI GCATC 5 cut(s) 40, 189, 972, 990, 1580
SfcI CTRYAG 3 cut(s) 990, 1770, 1831
Sfr303I CCGCGG 1 cut(s) 1690
SgrBI CCGCGG 1 cut(s) 1690
SinI GGWCC 2 cut(s) 132, 1137
SmiMI CAYNNNNRTG 7 cut(s) 119, 290, 1259, 1440, 1583, 1898, 1904
SmlI CTYRAG 1 cut(s) 1085
SmoI CTYRAG 1 cut(s) 1085
Sse9I AATT 1 cut(s) 1394
SspMI CTAG 2 cut(s) 449, 2116
StyD4I CCNGG 4 cut(s) 333, 374, 1875, 2028
StyI CCWWGG 3 cut(s) 967, 1584, 1702
TaaI ACNGT 6 cut(s) 148, 346, 1013, 1121, 1439, 1739
TaiI ACGT 2 cut(s) 246, 584
TaqI TCGA 6 cut(s) 155, 297, 536, 1182, 1549, 1794
TasI AATT 1 cut(s) 1394
TatI WGTACW 3 cut(s) 570, 1185, 1821
TauI GCSGC 4 cut(s) 127, 251, 309, 1692
TfiI GAWTC 5 cut(s) 888, 1179, 1864, 2000, 2099
Tru1I TTAA 2 cut(s) 578, 771
Tru9I TTAA 2 cut(s) 578, 771
TscAI CASTG 8 cut(s) 31, 70, 226, 397, 719, 1126, 1264, 1369
TseFI GTSAC 2 cut(s) 38, 160
TseI GCWGC 9 cut(s) 319, 434, 725, 728, 738, 1244, 1766, 1769, 2021
Tsp45I GTSAC 2 cut(s) 38, 160
TspRI CASTG 8 cut(s) 31, 70, 226, 397, 719, 1126, 1264, 1369
Van91I CCANNNNNTGG 2 cut(s) 764, 1303
VpaK11BI GGWCC 2 cut(s) 132, 1137
XagI CCTNNNNNAGG 1 cut(s) 17
XapI RAATTY 1 cut(s) 1394
XceI RCATGY 2 cut(s) 1036, 1903
XcmI CCANNNNNNNNNTGG 1 cut(s) 1611
XmiI GTMKAC 1 cut(s) 1029
XspI CTAG 2 cut(s) 449, 2116
ZrmI AGTACT 1 cut(s) 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.