Rmu_ssc0000144.1_g000013

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000144.1
OG0001164
Physical Location & Seq
Forward (+)
64969 .. 67664
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000144.1_g000013.1.cds

Sequence Viewer

Length: 2091 bp
atggagagaaagaagagcgatgactttgatcaggatggaaatcaagcacagaacaagattttggtggaagcggcattcaggaacaccccggtgccgccgtggacgaagcaaattactgtgagggcaatggccacgagctttattctgagcattgtcttcaacttcattgtttgcaagcttaacctcaccactggtgtcataccttcgctcaacgtcgccgccgggttgttgggttttgcggtgttgagagcttacaccatcgctctcaccaaggttggcctcttgaagcagcctttcactcgccaagaaaattgtgttatccaaacatgtgtggtcgcctcatccgggattgcattcagcagtggaaccgcaagttatatactgggtatgagtggtacgatagctgctcaaggagatgaaggcaacaccccaatcaacgtgaagaaccctcacattggatggatgattggatttctctttactgtcagcttcctcggcttgttttccattatgcccatgagaaagatgatgatcatggagtacaagttaacctacccaagtggaactgcaactgcatacctcattaacagttttcacacccccaaaggagccaagttagcaaagaaacaagttggcgtcctgttcaaaagcttctgctttagctttgtgtgggcctttttccaatggttctatacagccgctgatggctgcggatttgctagcttcccaacatttggtctccaagcttatgcacagaagttttactttgacttctcatcgacatacgttggtgtgggaatgatttgcccttacatggtgaacatctctttgcttgtcggatcaatactttcatggggaatcatgtggccgttgattgagaaacataaaggtaactggtacagtgctgatctctcagcgagcagtctccacggtatccaaggttacagggttttcattgctatagccatgatgcttggggatggtttataccatgtagcttacatgctctttgtgaccttccaaaatttatgttgtacacctaaaacaaaagaagaaatgaaattagagtcatcatccccaccgtccataaaccctgcaagtactgaagctggagtagaagttttgagtgaaaagtatgacgaggagcgaagagttgagtatttcttgaaagacgcaatttctcactgggttgcttttggtggatatgttgctcttgccattatatccatcattaccgtaccaagcattttccgccaattaaaatggtaccatatctttattgcttatttggttgctccagttttggcattctgtaattcctatggtactggtctcaccgactggtccctcgcctccaactatggaaaattcgccatcataatcttcagtgcttgggttggccctgaaggtggtgttgttgctgggcttgctgcttgtggtgtcatgatgagcattgtcgctactgcttctgatcttatgcaggacttcaagactggataccttacgctttcctctcctcgctccatgttcttcagccaagttcttggcacggccatgggttgtctcatgtcccctttggtattctggattttctacaaagcttatccactaggcgatccaaatggttcgtatcctgcaccgtttgcactaatgtaccgtggaatggctcttttaggagttgaaggcgttggttcacttcccaaaaattgtgtgaaacttgcagtctcattttttgtagctgctgttgtcataaatttgattactgagcttctaaagcgttatgagactaagtacaggttgtacagatttattcctagcccaatgtgcatggccatcccattttatcttggttcatactttgccattgacatgtgtttggggagcttgattctcttcttgtggcaaagaatgaacaaacaaaaggccgatgactttgcaccagcagtcgcatctggactcatttgcggggaatctttgtggggtattccagctgctatcctttcactggccaatgtgaaacctccgatctgtatgaagttcctttctgcttctgccaatgaaaaagtcgaaaaacttttatctggtcattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

696

Amino Acids

76.52

Weight (kDa)

8.9

Isoelectric Point (pI)

35.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1266
AccB1I GGYRCC 2 cut(s) 91, 1266
AccB7I CCANNNNNTGG 1 cut(s) 734
AciI CCGC 9 cut(s) 71, 95, 219, 239, 369, 699, 711, 1252, 1965
AclWI GGATC 2 cut(s) 847, 1610
AcoI YGGCCR 5 cut(s) 129, 866, 1551, 1830, 2007
AcsI RAATTY 3 cut(s) 1024, 1367, 1753
AcuI CTGAAG 4 cut(s) 1125, 1369, 1425, 1516
AcyI GRCGYC 1 cut(s) 636
AfiI CCNNNNNNNGG 7 cut(s) 345, 455, 734, 814, 1409, 1663, 2005
AflIII ACRYGT 2 cut(s) 326, 1869
AgsI TTSAA 6 cut(s) 160, 286, 646, 1168, 1489, 1682
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AleI CACNNNNGTG 1 cut(s) 89
Alw26I GTCTC 6 cut(s) 743, 929, 1337, 1568, 1729, 1778
AlwI GGATC 2 cut(s) 847, 1610
AlwNI CAGNNNCTG 1 cut(s) 701
AoxI GGCC 9 cut(s) 129, 277, 672, 866, 1399, 1551, 1830, 1923, 2007
ApeKI GCWGC 6 cut(s) 289, 404, 708, 1430, 1739, 1991
ApoI RAATTY 3 cut(s) 1024, 1367, 1753
Asp718I GGTACC 1 cut(s) 1266
AspS9I GGNCC 3 cut(s) 672, 1344, 1400
AsuC2I CCSGG 3 cut(s) 89, 223, 346
AsuHPI GGTGA 4 cut(s) 178, 259, 829, 1327
AsuNHI GCTAGC 1 cut(s) 719
AvaII GGWCC 1 cut(s) 1344
BaeI ACNNNNGTAYC 2 cut(s) 1637, 1670
BalI TGGCCA 3 cut(s) 131, 1832, 2009
BanI GGYRCC 2 cut(s) 91, 1266
BauI CACGAG 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 6 cut(s) 301, 391, 695, 1417, 1726, 1978
BccI CCATC 8 cut(s) 29, 266, 453, 698, 974, 1235, 1382, 1841
BceAI ACGGC 3 cut(s) 82, 853, 1566
BcgI CGANNNNNNTGC 2 cut(s) 1916, 1950
BciVI GTATCC 3 cut(s) 944, 1490, 1641
BclI TGATCA 2 cut(s) 28, 531
BcnI CCSGG 3 cut(s) 89, 223, 346
BcoDI GTCTC 6 cut(s) 743, 929, 1337, 1568, 1729, 1778
BfaI CTAG 3 cut(s) 720, 1610, 1815
BfmI CTRYAG 1 cut(s) 960
BfuI GTATCC 3 cut(s) 944, 1490, 1641
BmcAI AGTACT 1 cut(s) 1102
Bme1390I CCNGG 3 cut(s) 89, 223, 346
Bme18I GGWCC 1 cut(s) 1344
BmgT120I GGNCC 3 cut(s) 672, 1344, 1400
BmiI GGNNCC 5 cut(s) 93, 367, 610, 1268, 1346
BmrFI CCNGG 3 cut(s) 89, 223, 346
BmrI ACTGGG 2 cut(s) 392, 1195
BmsI GCATC 2 cut(s) 960, 1958
BmtI GCTAGC 1 cut(s) 723
BmuI ACTGGG 2 cut(s) 392, 1195
BpiI GAAGAC 1 cut(s) 148
BpmI CTGGAG 2 cut(s) 1131, 1281
BpuEI CTTGAG 1 cut(s) 393
BpuMI CCSGG 3 cut(s) 89, 223, 346
BsaBI GATNNNNATC 2 cut(s) 39, 530
BsaHI GRCGYC 1 cut(s) 636
BsaI GGTCTC 2 cut(s) 743, 1337
BsaJI CCNNGG 8 cut(s) 87, 98, 270, 493, 928, 937, 1554, 1657
BsaXI ACNNNNNCTCC 4 cut(s) 530, 560, 1104, 1134
Bsc4I CCNNNNNNNGG 7 cut(s) 345, 455, 734, 814, 1409, 1663, 2005
Bse1I ACTGG 9 cut(s) 196, 387, 899, 1190, 1298, 1333, 1346, 1498, 2010
Bse3DI GCAATG 2 cut(s) 132, 954
Bse8I GATNNNNATC 2 cut(s) 39, 530
BseDI CCNNGG 8 cut(s) 87, 98, 270, 493, 928, 937, 1554, 1657
BseGI GGATG 7 cut(s) 40, 341, 464, 468, 985, 1073, 1833
BseJI GATNNNNATC 2 cut(s) 39, 530
BseLI CCNNNNNNNGG 7 cut(s) 345, 455, 734, 814, 1409, 1663, 2005
BseMI GCAATG 2 cut(s) 132, 954
BseMII CTCAG 3 cut(s) 137, 927, 1755
BseNI ACTGG 9 cut(s) 196, 387, 899, 1190, 1298, 1333, 1346, 1498, 2010
BseRI GAGGAG 2 cut(s) 1157, 1506
BseXI GCAGC 6 cut(s) 301, 391, 695, 1417, 1726, 1978
BseYI CCCAGC 1 cut(s) 1421
BsgI GTGCAG 1 cut(s) 1620
BshFI GGCC 9 cut(s) 131, 279, 674, 868, 1401, 1553, 1832, 1925, 2009
BshNI GGYRCC 2 cut(s) 91, 1266
BsiSI CCGG 3 cut(s) 89, 222, 345
BslFI GGGAC 2 cut(s) 1330, 1555
BslI CCNNNNNNNGG 7 cut(s) 345, 455, 734, 814, 1409, 1663, 2005
BsmAI GTCTC 6 cut(s) 743, 929, 1337, 1568, 1729, 1778
BsmFI GGGAC 2 cut(s) 1330, 1555
BsmI GAATGC 3 cut(s) 74, 353, 1307
BsnI GGCC 9 cut(s) 131, 279, 674, 868, 1401, 1553, 1832, 1925, 2009
Bso31I GGTCTC 2 cut(s) 743, 1337
Bsp1407I TGTACA 2 cut(s) 1034, 1800
Bsp143I GATC 7 cut(s) 28, 531, 839, 907, 1471, 1615, 2025
Bsp19I CCATGG 1 cut(s) 1554
BspACI CCGC 9 cut(s) 71, 95, 219, 239, 369, 699, 711, 1252, 1965
BspANI GGCC 9 cut(s) 131, 279, 674, 868, 1401, 1553, 1832, 1925, 2009
BspCNI CTCAG 3 cut(s) 138, 926, 1756
BspHI TCATGA 1 cut(s) 1443
BspLI GGNNCC 5 cut(s) 93, 367, 610, 1268, 1346
BspOI GCTAGC 1 cut(s) 723
BspPI GGATC 2 cut(s) 847, 1610
BspQI GCTCTTC 1 cut(s) 8
BspT107I GGYRCC 2 cut(s) 91, 1266
BspTNI GGTCTC 2 cut(s) 743, 1337
BsrDI GCAATG 2 cut(s) 132, 954
BsrGI TGTACA 2 cut(s) 1034, 1800
BsrI ACTGG 9 cut(s) 196, 387, 899, 1190, 1298, 1333, 1346, 1498, 2010
BssECI CCNNGG 8 cut(s) 87, 98, 270, 493, 928, 937, 1554, 1657
BssMI GATC 7 cut(s) 28, 531, 839, 907, 1471, 1615, 2025
BssNI GRCGYC 1 cut(s) 636
BssSI CACGAG 1 cut(s) 133
BssT1I CCWWGG 3 cut(s) 270, 937, 1554
Bst2BI CACGAG 1 cut(s) 133
Bst4CI ACNGT 9 cut(s) 118, 484, 590, 902, 932, 1083, 1237, 1641, 1658
Bst6I CTCTTC 3 cut(s) 8, 1144, 1898
BstACI GRCGYC 1 cut(s) 636
BstAPI GCANNNNNTGC 1 cut(s) 1643
BstAUI TGTACA 2 cut(s) 1034, 1800
BstC8I GCNNGC 4 cut(s) 176, 721, 919, 1428
BstDEI CTNAG 4 cut(s) 146, 913, 1764, 1788
BstDSI CCRYGG 4 cut(s) 98, 928, 1554, 1657
BstF5I GGATG 7 cut(s) 40, 341, 464, 468, 985, 1073, 1833
BstKTI GATC 7 cut(s) 31, 534, 842, 910, 1474, 1618, 2028
BstMAI GTCTC 6 cut(s) 743, 929, 1337, 1568, 1729, 1778
BstMBI GATC 7 cut(s) 28, 531, 839, 907, 1471, 1615, 2025
BstMWI GCNNNNNNNGC 7 cut(s) 495, 617, 1251, 1427, 1643, 1774, 1824
BstNSI RCATGY 3 cut(s) 330, 1006, 1873
BstSCI CCNGG 3 cut(s) 87, 221, 344
BstSFI CTRYAG 1 cut(s) 960
BstV1I GCAGC 6 cut(s) 301, 391, 695, 1417, 1726, 1978
BstV2I GAAGAC 1 cut(s) 148
BstXI CCANNNNNNTGG 1 cut(s) 1544
BsuI GTATCC 3 cut(s) 944, 1490, 1641
BsuRI GGCC 9 cut(s) 131, 279, 674, 868, 1401, 1553, 1832, 1925, 2009
BtgI CCRYGG 4 cut(s) 98, 928, 1554, 1657
BtgZI GCGATG 2 cut(s) 33, 244
BtsCI GGATG 7 cut(s) 40, 341, 464, 468, 985, 1073, 1833
BtsI GCAGTG 1 cut(s) 367
BtsIMutI CAGTG 6 cut(s) 189, 367, 907, 1183, 1393, 2003
Cac8I GCNNGC 4 cut(s) 176, 721, 919, 1428
CaiI CAGNNNCTG 1 cut(s) 701
CciI TCATGA 1 cut(s) 1443
Cfr13I GGNCC 3 cut(s) 672, 1344, 1400
CseI GACGC 2 cut(s) 625, 1181
DdeI CTNAG 4 cut(s) 146, 913, 1764, 1788
DpnI GATC 7 cut(s) 30, 533, 841, 909, 1473, 1617, 2027
DpnII GATC 7 cut(s) 28, 531, 839, 907, 1471, 1615, 2025
EaeI YGGCCR 5 cut(s) 129, 866, 1551, 1830, 2007
Eam1104I CTCTTC 3 cut(s) 8, 1144, 1898
EarI CTCTTC 3 cut(s) 8, 1144, 1898
EciI GGCGGA 1 cut(s) 1241
Eco130I CCWWGG 3 cut(s) 270, 937, 1554
Eco31I GGTCTC 2 cut(s) 743, 1337
Eco47I GGWCC 1 cut(s) 1344
Eco57I CTGAAG 4 cut(s) 1125, 1369, 1425, 1516
EcoT14I CCWWGG 3 cut(s) 270, 937, 1554
ErhI CCWWGG 3 cut(s) 270, 937, 1554
FalI AAGNNNNNCTT 2 cut(s) 749, 781
FaqI GGGAC 2 cut(s) 1330, 1555
FauI CCCGC 1 cut(s) 1958
FbaI TGATCA 2 cut(s) 28, 531
FokI GGATG 7 cut(s) 47, 328, 471, 475, 992, 1060, 1820
FspBI CTAG 3 cut(s) 720, 1610, 1815
GsaI CCCAGC 1 cut(s) 1425
GsuI CTGGAG 2 cut(s) 1131, 1281
HaeIII GGCC 9 cut(s) 131, 279, 674, 868, 1401, 1553, 1832, 1925, 2009
HapII CCGG 3 cut(s) 89, 222, 345
HgaI GACGC 2 cut(s) 625, 1181
Hin1I GRCGYC 1 cut(s) 636
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HindIII AAGCTT 4 cut(s) 176, 649, 744, 1599
HinfI GANTC 5 cut(s) 858, 1067, 1888, 1956, 1970
HpaI GTTAAC 1 cut(s) 549
HpaII CCGG 3 cut(s) 89, 222, 345
HphI GGTGA 4 cut(s) 178, 259, 829, 1327
Hpy166II GTNNAC 5 cut(s) 102, 549, 820, 1037, 1694
Hpy188I TCNGA 4 cut(s) 147, 839, 1471, 2025
Hpy188III TCNNGA 8 cut(s) 32, 79, 283, 1165, 1444, 1489, 1585, 1953
Hpy8I GTNNAC 5 cut(s) 102, 549, 820, 1037, 1694
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 5 cut(s) 213, 413, 1027, 1400, 1676
HpyCH4III ACNGT 9 cut(s) 118, 484, 590, 902, 932, 1083, 1237, 1641, 1658
HpyCH4IV ACGT 3 cut(s) 213, 438, 786
HpyF10VI GCNNNNNNNGC 7 cut(s) 495, 617, 1251, 1427, 1643, 1774, 1824
HpyF3I CTNAG 4 cut(s) 146, 913, 1764, 1788
HpySE526I ACGT 3 cut(s) 213, 438, 786
Hsp92I GRCGYC 1 cut(s) 636
KpnI GGTACC 1 cut(s) 1270
Ksp22I TGATCA 2 cut(s) 28, 531
KspAI GTTAAC 1 cut(s) 549
Kzo9I GATC 7 cut(s) 28, 531, 839, 907, 1471, 1615, 2025
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 5 cut(s) 608, 1144, 1300, 1526, 1881
Lsp1109I GCAGC 6 cut(s) 301, 391, 695, 1417, 1726, 1978
LweI GCATC 2 cut(s) 960, 1958
MaeI CTAG 3 cut(s) 720, 1610, 1815
MaeII ACGT 3 cut(s) 213, 438, 786
MaeIII GTNAC 3 cut(s) 890, 941, 1012
MalI GATC 7 cut(s) 30, 533, 841, 909, 1473, 1617, 2027
MboI GATC 7 cut(s) 28, 531, 839, 907, 1471, 1615, 2025
MboII GAAGA 8 cut(s) 25, 148, 454, 1064, 1161, 1375, 1522, 1885
MlsI TGGCCA 3 cut(s) 131, 1832, 2009
MluNI TGGCCA 3 cut(s) 131, 1832, 2009
MlyI GAGTC 2 cut(s) 1076, 1950
MmeI TCCRAC 2 cut(s) 817, 1380
Mox20I TGGCCA 3 cut(s) 131, 1832, 2009
MscI TGGCCA 3 cut(s) 131, 1832, 2009
MseI TTAA 4 cut(s) 180, 548, 585, 1259
MslI CAYNNNNRTG 3 cut(s) 89, 1553, 1868
Msp20I TGGCCA 3 cut(s) 131, 1832, 2009
MspA1I CMGCKG 2 cut(s) 701, 1991
MspI CCGG 3 cut(s) 89, 222, 345
MspR9I CCNGG 3 cut(s) 89, 223, 346
Mva1269I GAATGC 3 cut(s) 74, 353, 1307
MwoI GCNNNNNNNGC 7 cut(s) 495, 617, 1251, 1427, 1643, 1774, 1824
NciI CCSGG 3 cut(s) 89, 223, 346
NcoI CCATGG 1 cut(s) 1554
NdeII GATC 7 cut(s) 28, 531, 839, 907, 1471, 1615, 2025
NheI GCTAGC 1 cut(s) 719
NlaIV GGNNCC 5 cut(s) 93, 367, 610, 1268, 1346
NmeAIII GCCGAG 1 cut(s) 474
NmuCI GTSAC 1 cut(s) 1012
NspI RCATGY 3 cut(s) 330, 1006, 1873
OliI CACNNNNGTG 1 cut(s) 89
PagI TCATGA 1 cut(s) 1443
PciI ACATGT 2 cut(s) 326, 1869
PciSI GCTCTTC 1 cut(s) 8
PctI GAATGC 3 cut(s) 74, 353, 1307
PfeI GAWTC 3 cut(s) 858, 1888, 1970
PflMI CCANNNNNTGG 1 cut(s) 734
PfoI TCCNGGA 1 cut(s) 344
PleI GAGTC 2 cut(s) 1075, 1950
PpsI GAGTC 2 cut(s) 1075, 1950
PscI ACATGT 2 cut(s) 326, 1869
PspFI CCCAGC 1 cut(s) 1421
PspN4I GGNNCC 5 cut(s) 93, 367, 610, 1268, 1346
PspPI GGNCC 3 cut(s) 672, 1344, 1400
PstNI CAGNNNCTG 1 cut(s) 701
PvuII CAGCTG 1 cut(s) 1991
RseI CAYNNNNRTG 3 cut(s) 89, 1553, 1868
SapI GCTCTTC 1 cut(s) 8
SaqAI TTAA 4 cut(s) 180, 548, 585, 1259
Sau3AI GATC 7 cut(s) 28, 531, 839, 907, 1471, 1615, 2025
Sau96I GGNCC 3 cut(s) 672, 1344, 1400
ScaI AGTACT 1 cut(s) 1102
SchI GAGTC 2 cut(s) 1076, 1950
ScrFI CCNGG 3 cut(s) 89, 223, 346
SfaNI GCATC 2 cut(s) 960, 1958
SfcI CTRYAG 1 cut(s) 960
SinI GGWCC 1 cut(s) 1344
SmiMI CAYNNNNRTG 3 cut(s) 89, 1553, 1868
SmlI CTYRAG 1 cut(s) 408
SmoI CTYRAG 1 cut(s) 408
SsiI CCGC 9 cut(s) 71, 95, 219, 239, 369, 699, 711, 1252, 1965
SspMI CTAG 3 cut(s) 720, 1610, 1815
StyD4I CCNGG 3 cut(s) 87, 221, 344
StyI CCWWGG 3 cut(s) 270, 937, 1554
TaaI ACNGT 9 cut(s) 118, 484, 590, 902, 932, 1083, 1237, 1641, 1658
TaiI ACGT 3 cut(s) 216, 441, 789
TaqI TCGA 2 cut(s) 779, 2067
TatI WGTACW 5 cut(s) 540, 1034, 1100, 1791, 1800
TauI GCSGC 4 cut(s) 74, 97, 221, 701
TfiI GAWTC 3 cut(s) 858, 1888, 1970
Tru1I TTAA 4 cut(s) 180, 548, 585, 1259
Tru9I TTAA 4 cut(s) 180, 548, 585, 1259
TscAI CASTG 6 cut(s) 196, 367, 907, 1190, 1393, 2010
TseFI GTSAC 1 cut(s) 1012
TseI GCWGC 6 cut(s) 289, 404, 708, 1430, 1739, 1991
Tsp45I GTSAC 1 cut(s) 1012
TspDTI ATGAA 9 cut(s) 154, 432, 840, 943, 1073, 1842, 1925, 2048, 2073
TspRI CASTG 6 cut(s) 196, 367, 907, 1190, 1393, 2010
Van91I CCANNNNNTGG 1 cut(s) 734
VpaK11BI GGWCC 1 cut(s) 1344
XapI RAATTY 3 cut(s) 1024, 1367, 1753
XceI RCATGY 3 cut(s) 330, 1006, 1873
XspI CTAG 3 cut(s) 720, 1610, 1815
ZrmI AGTACT 1 cut(s) 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.