Rh5AG211400

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001164
Physical Location & Seq
Forward (+)
24944105 .. 24952412
8308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG211400.1

Sequence Viewer

Length: 2142 bp
ATGAAGACTGAAGATTTCGACAATGATCAAGCTGGAACACAAATGGTTCAGGTTCAAAATGACTCGACCGAAAAGGCATTCAGAGACACGGAGGTGCCTCCATGGCACAAGCAAATAACTGTGAGGTCTATGGTCACAAGTTTTTTTCTTAGCATCATCTTCAACTTCATCGTGTGCAAGCTCAATCTCACCACTGGTGTAATACCATCTCTAAATGTCGCGGCGGGGCTGCTGGGATTCGCAATCATGAAAGGATACACTGCCTTACTTGACAAGTTAGGTCTGTTGAAGCAGCCATTCACTCGTCAAGAAAACACAGTGATTCAAACCTGTGTTGTGGCCTCGTCTGGGATAGCCTTTAGCAGCGGCATGGGGAGTTATTTACTAGGGATGAGCCCTCTAGTAGCTGCTCAGGGAGATGCAGGCAACACTCTAATCAACATCAAGAGGCTTGCGATTCCGTGGATGACAGGATATCTCTTCACAGTCAGCTTCGTTGGGCTGTTTTCAATGATACCCTTGAGAAAGATGATGATCATTGATAGAAAACTAACGTACCCAAGTGGAACAGCAACAGCGCACCTCATCAACAGCTTCCACACCCCCAAAGGAGCCAAGAAGGCAGCGAGACAGGTTGCTGTTCTCTTCAAGAGCTGCTGCGGCAGCTTTGCGTGGTCCTTTTTTCAGTGGTTTTTCGCGGCTTCGGATGGCTGTGGGTTTACTAGCTTTCCAATTTTCGGTCTCAAAGCCTACAAACAGAGGTTTTACTTCGACTTCTCAACCACATATATTGGTTGTGGAATGATCACCTCTTACATGGTGAACGTATCCATGCTCGTTGGAGCTGTGATATCATGGGGAATCATGTGGCCTTTGATTGAACAAAAGAAAGGTATCTGGTATGGTGCTCATCTTTCTGCAAGTAGTCTTCATGGAATCCAAGGTTACAGGGTCTTTATTTCCATAGCAATGATACTTGGTGATGGTCTATACCATGTTATGTACCATGCTGGTTATGCGCTTTTCATTATCATAAAAAGTCGGTTCAGCAATAAACCAGAAGAAAAGAAAGTAGAGTTGTCATCGCCACCACCTCTACCATTAACCAATATGGATGCTGGATTGCGCTCATCAGAAACTGAACTGAGTGAAAACTACGACGAGCAAAGAAGAACTGAGTACTTCTTGAAGGACCAAATTCCGCGCCGCTTTGCTGTCTCTGGCTACGTTGCTCTTGCAGCCATATCTGCTAGTGTCCTTCCATTACTGTTTCACCAACTAAAATGGTATCATGTTGCGCTAGCTTACTTCATCGCTCCTGTTCTGGCCTTCTGCAATGCCTATGGCTGCGGCCTCACAGATTGGTCCCTCGCTTCCAACTACGGCAAAATCGCAATCCTGATCTTCAGTGCTTGTGTTGGCCCTGAAAAAGGTGGTGTCATTGCTGGCCTCGCTTCTTGTGGTGTAATGATGAGCATTGTCTCAACAGCCTCGGATCTCATGCAAGACTTCAAGACTGGATATCTAACTCTCGCGTCTCCTCGATCCATGTTCGTTAGCCAAGTTTTCGGGACAGCCATGGGGTGTGTCATGTCGCCTATGATATTCTGGTTCTTCTACAAAGCTTACCGAGTTGGCGATCCAACGGGCTCGTATCCTGCACCCTATGGCCTAATGTACCGTGGAATAGCACTCCTCGGAGTTGAGGGCTTTGGCTCACTCCCCAAACACTGCGTTGCACTTGCAGTCTCCTTTTTCGCATTTGCCATTGCCATTAACGTAGTAACCGAGCTTTTGAAGCGTTACGAGACCAAGTACAAGATCTACAGATTTGTTCCAAATGCAATGGCCATGGCAGTCCCGTTCTATCTCGGCTCCTACTTCGTGATTGACATGTGCATAGGAAGCTTGATCCTTTTCGGCTGGAAGTTATGGAACAAACAACAGGCTGAGGATTTAGCACCGGCGGTTGCATCTGGTCTCATTTGTGGCGAATTGAGATCAATCGCAAATAAACAGAACAAATATGTCATCTTTCCTTCAGATGACGATGATGATGATGATTCTCACGTTCATGAAAATATATGGTATGTTATCAGAAATAGAGGAGTGAATCAGGGTAAAGTTTTGAAACCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

713

Amino Acids

78.91

Weight (kDa)

8.93

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 38 - 665 1.3e-148 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 94
AccII CGCG 4 cut(s) 221, 698, 1204, 1535
AciI CCGC 9 cut(s) 221, 224, 366, 660, 698, 1202, 1207, 1350, 1967
AclWI GGATC 4 cut(s) 1503, 1539, 1634, 1906
AcoI YGGCCR 1 cut(s) 1848
AcsI RAATTY 1 cut(s) 1197
AcuI CTGAAG 3 cut(s) 30, 1390, 2025
AfaI GTAC 5 cut(s) 557, 1004, 1181, 1679, 1817
AfiI CCNNNNNNNGG 3 cut(s) 348, 737, 1430
AflIII ACRYGT 1 cut(s) 1893
AleI CACNNNNGTG 1 cut(s) 92
Alw21I GWGCWC 1 cut(s) 910
Alw26I GTCTC 9 cut(s) 78, 622, 746, 1222, 1486, 1542, 1753, 1802, 1985
AlwI GGATC 4 cut(s) 1503, 1539, 1634, 1906
AlwNI CAGNNNCTG 1 cut(s) 1139
AoxI GGCC 8 cut(s) 339, 869, 1326, 1351, 1420, 1447, 1669, 1848
ApoI RAATTY 1 cut(s) 1197
AspLEI GCGC 5 cut(s) 580, 1021, 1128, 1206, 1300
AspS9I GGNCC 4 cut(s) 675, 1192, 1365, 1421
AsuHPI GGTGA 5 cut(s) 181, 799, 832, 992, 1265
AsuNHI GCTAGC 1 cut(s) 1300
AvaII GGWCC 3 cut(s) 675, 1192, 1365
BaeI ACNNNNGTAYC 2 cut(s) 539, 572
BalI TGGCCA 1 cut(s) 1850
BanI GGYRCC 1 cut(s) 94
BanII GRGCYC 2 cut(s) 398, 1652
BbsI GAAGAC 2 cut(s) 11, 920
Bbv12I GWGCWC 1 cut(s) 910
BbvCI CCTCAGC 1 cut(s) 1950
BccI CCATC 3 cut(s) 214, 701, 977
BceAI ACGGC 1 cut(s) 1399
BciVI GTATCC 3 cut(s) 248, 838, 1665
BclI TGATCA 3 cut(s) 25, 534, 804
BcoDI GTCTC 9 cut(s) 78, 622, 746, 1222, 1486, 1542, 1753, 1802, 1985
BfaI CTAG 5 cut(s) 386, 401, 723, 1251, 1301
BfmI CTRYAG 1 cut(s) 1825
BfuI GTATCC 3 cut(s) 248, 838, 1665
BglI GCCNNNNNGGC 2 cut(s) 103, 620
BglII AGATCT 1 cut(s) 1821
BmcAI AGTACT 1 cut(s) 1181
Bme18I GGWCC 3 cut(s) 675, 1192, 1365
BmgT120I GGNCC 4 cut(s) 675, 1192, 1365, 1421
BmiI GGNNCC 4 cut(s) 96, 613, 1367, 1876
BmsI GCATC 4 cut(s) 162, 409, 1105, 1982
BmtI GCTAGC 1 cut(s) 1304
BpiI GAAGAC 2 cut(s) 11, 920
Bpu10I CCTNAGC 2 cut(s) 411, 1950
BpuEI CTTGAG 1 cut(s) 541
BsaBI GATNNNNATC 1 cut(s) 533
BsaI GGTCTC 3 cut(s) 746, 1802, 1985
BsaJI CCNNGG 8 cut(s) 101, 461, 940, 1491, 1578, 1681, 1696, 1851
Bsc4I CCNNNNNNNGG 3 cut(s) 348, 737, 1430
Bse118I RCCGGY 1 cut(s) 1963
Bse1I ACTGG 2 cut(s) 199, 1522
Bse3DI GCAATG 5 cut(s) 975, 1342, 1440, 1767, 1851
Bse8I GATNNNNATC 1 cut(s) 533
BseDI CCNNGG 8 cut(s) 101, 461, 940, 1491, 1578, 1681, 1696, 1851
BseGI GGATG 4 cut(s) 396, 471, 712, 1120
BseJI GATNNNNATC 1 cut(s) 533
BseLI CCNNNNNNNGG 3 cut(s) 348, 737, 1430
BseMI GCAATG 5 cut(s) 975, 1342, 1440, 1767, 1851
BseMII CTCAG 4 cut(s) 425, 1136, 1167, 1941
BseNI ACTGG 2 cut(s) 199, 1522
BseRI GAGGAG 3 cut(s) 1530, 1685, 2121
BseYI CCCAGC 1 cut(s) 232
BsgI GTGCAG 1 cut(s) 1644
Bsh1236I CGCG 4 cut(s) 221, 698, 1204, 1535
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
BshNI GGYRCC 1 cut(s) 94
BsiEI CGRYCG 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 910
BsiSI CCGG 1 cut(s) 1964
BslFI GGGAC 3 cut(s) 1351, 1585, 1844
BslI CCNNNNNNNGG 3 cut(s) 348, 737, 1430
BsmAI GTCTC 9 cut(s) 78, 622, 746, 1222, 1486, 1542, 1753, 1802, 1985
BsmBI CGTCTC 1 cut(s) 1542
BsmFI GGGAC 3 cut(s) 1351, 1585, 1844
BsmI GAATGC 1 cut(s) 77
BsnI GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
Bso31I GGTCTC 3 cut(s) 746, 1802, 1985
Bsp1286I GDGCHC 3 cut(s) 398, 910, 1652
Bsp19I CCATGG 3 cut(s) 101, 1578, 1851
BspACI CCGC 9 cut(s) 221, 224, 366, 660, 698, 1202, 1207, 1350, 1967
BspANI GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
BspCNI CTCAG 4 cut(s) 424, 1137, 1168, 1942
BspFNI CGCG 4 cut(s) 221, 698, 1204, 1535
BspHI TCATGA 2 cut(s) 246, 2074
BspLI GGNNCC 4 cut(s) 96, 613, 1367, 1876
BspOI GCTAGC 1 cut(s) 1304
BspPI GGATC 4 cut(s) 1503, 1539, 1634, 1906
BspT107I GGYRCC 1 cut(s) 94
BspTNI GGTCTC 3 cut(s) 746, 1802, 1985
BsrDI GCAATG 5 cut(s) 975, 1342, 1440, 1767, 1851
BsrFI RCCGGY 1 cut(s) 1963
BsrI ACTGG 2 cut(s) 199, 1522
BssAI RCCGGY 1 cut(s) 1963
BssECI CCNNGG 8 cut(s) 101, 461, 940, 1491, 1578, 1681, 1696, 1851
BssT1I CCWWGG 4 cut(s) 101, 940, 1578, 1851
Bst4CI ACNGT 5 cut(s) 121, 319, 487, 1269, 1682
Bst6I CTCTTC 2 cut(s) 485, 650
BstC8I GCNNGC 5 cut(s) 179, 424, 453, 1302, 1447
BstDEI CTNAG 5 cut(s) 149, 411, 1145, 1176, 1950
BstDSI CCRYGG 5 cut(s) 101, 461, 1578, 1681, 1851
BstENI CCTNNNNNAGG 1 cut(s) 1428
BstF5I GGATG 4 cut(s) 396, 471, 712, 1120
BstFNI CGCG 4 cut(s) 221, 698, 1204, 1535
BstHHI GCGC 5 cut(s) 580, 1021, 1128, 1206, 1300
BstMAI GTCTC 9 cut(s) 78, 622, 746, 1222, 1486, 1542, 1753, 1802, 1985
BstMCI CGRYCG 1 cut(s) 69
BstNSI RCATGY 1 cut(s) 1897
BstSFI CTRYAG 1 cut(s) 1825
BstUI CGCG 4 cut(s) 221, 698, 1204, 1535
BstV2I GAAGAC 2 cut(s) 11, 920
BstX2I RGATCY 2 cut(s) 1495, 1821
BstYI RGATCY 2 cut(s) 1495, 1821
BsuI GTATCC 3 cut(s) 248, 838, 1665
BsuRI GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
BtgI CCRYGG 5 cut(s) 101, 461, 1578, 1681, 1851
BtgZI GCGATG 2 cut(s) 1068, 1297
BtsCI GGATG 4 cut(s) 396, 471, 712, 1120
BtsI GCAGTG 2 cut(s) 258, 1729
BtsIMutI CAGTG 6 cut(s) 192, 258, 324, 692, 1414, 1729
Cac8I GCNNGC 5 cut(s) 179, 424, 453, 1302, 1447
CaiI CAGNNNCTG 1 cut(s) 1139
CciI TCATGA 2 cut(s) 246, 2074
CfoI GCGC 5 cut(s) 580, 1021, 1128, 1206, 1300
Cfr10I RCCGGY 1 cut(s) 1963
Cfr13I GGNCC 4 cut(s) 675, 1192, 1365, 1421
CseI GACGC 1 cut(s) 1524
Csp6I GTAC 5 cut(s) 556, 1003, 1180, 1678, 1816
CspCI CAANNNNNGTGG 2 cut(s) 772, 807
CviQI GTAC 5 cut(s) 556, 1003, 1180, 1678, 1816
DdeI CTNAG 5 cut(s) 149, 411, 1145, 1176, 1950
EaeI YGGCCR 1 cut(s) 1848
Eam1104I CTCTTC 2 cut(s) 485, 650
EarI CTCTTC 2 cut(s) 485, 650
Eco130I CCWWGG 4 cut(s) 101, 940, 1578, 1851
Eco24I GRGCYC 2 cut(s) 398, 1652
Eco31I GGTCTC 3 cut(s) 746, 1802, 1985
Eco32I GATATC 3 cut(s) 476, 852, 1523
Eco47I GGWCC 3 cut(s) 675, 1192, 1365
Eco57I CTGAAG 3 cut(s) 30, 1390, 2025
EcoNI CCTNNNNNAGG 1 cut(s) 1428
EcoRV GATATC 3 cut(s) 476, 852, 1523
EcoT14I CCWWGG 4 cut(s) 101, 940, 1578, 1851
EcoT38I GRGCYC 2 cut(s) 398, 1652
ErhI CCWWGG 4 cut(s) 101, 940, 1578, 1851
Esp3I CGTCTC 1 cut(s) 1542
FaqI GGGAC 3 cut(s) 1351, 1585, 1844
FauI CCCGC 1 cut(s) 217
FbaI TGATCA 3 cut(s) 25, 534, 804
FokI GGATG 4 cut(s) 403, 478, 719, 1127
FriOI GRGCYC 2 cut(s) 398, 1652
FspBI CTAG 5 cut(s) 386, 401, 723, 1251, 1301
GlaI GCGC 5 cut(s) 579, 1020, 1127, 1205, 1299
GsaI CCCAGC 1 cut(s) 236
HaeIII GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
HapII CCGG 1 cut(s) 1964
HgaI GACGC 1 cut(s) 1524
HhaI GCGC 5 cut(s) 580, 1021, 1128, 1206, 1300
Hin6I GCGC 5 cut(s) 578, 1019, 1126, 1204, 1298
HinP1I GCGC 5 cut(s) 578, 1019, 1126, 1204, 1298
HindIII AAGCTT 2 cut(s) 1623, 1906
HinfI GANTC 8 cut(s) 62, 237, 322, 457, 861, 936, 2063, 2113
HpaII CCGG 1 cut(s) 1964
HphI GGTGA 5 cut(s) 181, 799, 832, 992, 1265
Hpy166II GTNNAC 2 cut(s) 720, 823
Hpy188I TCNGA 7 cut(s) 83, 706, 1135, 1495, 1700, 2044, 2099
Hpy8I GTNNAC 2 cut(s) 720, 823
Hpy99I CGWCG 1 cut(s) 1163
HpyAV CCTTC 5 cut(s) 613, 1183, 1268, 1339, 2049
HpyCH4III ACNGT 5 cut(s) 121, 319, 487, 1269, 1682
HpyCH4IV ACGT 5 cut(s) 554, 825, 1227, 1779, 2070
HpyF3I CTNAG 5 cut(s) 149, 411, 1145, 1176, 1950
HpySE526I ACGT 5 cut(s) 554, 825, 1227, 1779, 2070
HspAI GCGC 5 cut(s) 578, 1019, 1126, 1204, 1298
Ksp22I TGATCA 3 cut(s) 25, 534, 804
LmnI GCTCC 4 cut(s) 611, 842, 1321, 1880
LweI GCATC 4 cut(s) 162, 409, 1105, 1982
MaeI CTAG 5 cut(s) 386, 401, 723, 1251, 1301
MaeII ACGT 5 cut(s) 554, 825, 1227, 1779, 2070
MaeIII GTNAC 4 cut(s) 133, 944, 1783, 1802
MflI RGATCY 2 cut(s) 1495, 1821
MhlI GDGCHC 3 cut(s) 398, 910, 1652
MlsI TGGCCA 1 cut(s) 1850
MluCI AATT 3 cut(s) 732, 1197, 1994
MluNI TGGCCA 1 cut(s) 1850
MlyI GAGTC 1 cut(s) 56
MmeI TCCRAC 3 cut(s) 820, 1401, 1667
Mox20I TGGCCA 1 cut(s) 1850
MscI TGGCCA 1 cut(s) 1850
MseI TTAA 2 cut(s) 1103, 1776
MslI CAYNNNNRTG 3 cut(s) 92, 968, 2073
Msp20I TGGCCA 1 cut(s) 1850
MspA1I CMGCKG 1 cut(s) 366
MspI CCGG 1 cut(s) 1964
Mva1269I GAATGC 1 cut(s) 77
MvnI CGCG 4 cut(s) 221, 698, 1204, 1535
NcoI CCATGG 3 cut(s) 101, 1578, 1851
NheI GCTAGC 1 cut(s) 1300
NlaIV GGNNCC 4 cut(s) 96, 613, 1367, 1876
NmeAIII GCCGAG 1 cut(s) 1851
NmuCI GTSAC 1 cut(s) 133
NspI RCATGY 1 cut(s) 1897
OliI CACNNNNGTG 1 cut(s) 92
PagI TCATGA 2 cut(s) 246, 2074
PciI ACATGT 1 cut(s) 1893
PcsI WCGNNNNNNNCGW 1 cut(s) 1785
PctI GAATGC 1 cut(s) 77
PfeI GAWTC 7 cut(s) 237, 322, 457, 861, 936, 2063, 2113
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
PscI ACATGT 1 cut(s) 1893
PspFI CCCAGC 1 cut(s) 232
PspN4I GGNNCC 4 cut(s) 96, 613, 1367, 1876
PspPI GGNCC 4 cut(s) 675, 1192, 1365, 1421
PstNI CAGNNNCTG 1 cut(s) 1139
PsuI RGATCY 2 cut(s) 1495, 1821
RsaI GTAC 5 cut(s) 557, 1004, 1181, 1679, 1817
RsaNI GTAC 5 cut(s) 556, 1003, 1180, 1678, 1816
RseI CAYNNNNRTG 3 cut(s) 92, 968, 2073
SaqAI TTAA 2 cut(s) 1103, 1776
Sau96I GGNCC 4 cut(s) 675, 1192, 1365, 1421
ScaI AGTACT 1 cut(s) 1181
SchI GAGTC 1 cut(s) 56
SduI GDGCHC 3 cut(s) 398, 910, 1652
SfaNI GCATC 4 cut(s) 162, 409, 1105, 1982
SfcI CTRYAG 1 cut(s) 1825
SgrAI CRCCGGYG 1 cut(s) 1963
SinI GGWCC 3 cut(s) 675, 1192, 1365
SmiMI CAYNNNNRTG 3 cut(s) 92, 968, 2073
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
Sse9I AATT 3 cut(s) 732, 1197, 1994
SsiI CCGC 9 cut(s) 221, 224, 366, 660, 698, 1202, 1207, 1350, 1967
SspMI CTAG 5 cut(s) 386, 401, 723, 1251, 1301
StyI CCWWGG 4 cut(s) 101, 940, 1578, 1851
TaaI ACNGT 5 cut(s) 121, 319, 487, 1269, 1682
TaiI ACGT 5 cut(s) 557, 828, 1230, 1782, 2073
TaqI TCGA 4 cut(s) 18, 65, 771, 1543
TaqII GACCGA 2 cut(s) 83, 728
TasI AATT 3 cut(s) 732, 1197, 1994
TatI WGTACW 2 cut(s) 1179, 1815
TauI GCSGC 6 cut(s) 224, 369, 663, 701, 1209, 1353
TfiI GAWTC 7 cut(s) 237, 322, 457, 861, 936, 2063, 2113
Tru1I TTAA 2 cut(s) 1103, 1776
Tru9I TTAA 2 cut(s) 1103, 1776
TscAI CASTG 6 cut(s) 199, 265, 324, 692, 1414, 1736
TseFI GTSAC 1 cut(s) 133
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 8 cut(s) 17, 157, 263, 920, 1015, 1300, 2063, 2091
TspGWI ACGGA 2 cut(s) 104, 450
TspRI CASTG 6 cut(s) 199, 265, 324, 692, 1414, 1736
VpaK11BI GGWCC 3 cut(s) 675, 1192, 1365
XagI CCTNNNNNAGG 1 cut(s) 1428
XapI RAATTY 1 cut(s) 1197
XceI RCATGY 1 cut(s) 1897
XspI CTAG 5 cut(s) 386, 401, 723, 1251, 1301
ZrmI AGTACT 1 cut(s) 1181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.