Rh5BG210500

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001164
Physical Location & Seq
Reverse (-)
24414131 .. 24416519
2389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG210500.1

Sequence Viewer

Length: 2124 bp
ATGAAGACTGAAGATTTCGACAATGATCAAGCTGGAACACAAATGGTTCAGGCTCAAAATGACTCGACCGAAAAGGCATTCAGAGACACGGAGGTGCCTCCATGGCACAAGCAAATAACTGTGAGGTCTATGGTCACAAGTTTTTTTCTTAGCATCATCTTCAACTTCATCGTGTGCAAGCTCAATCTCACCACTGGTGTAATACCATCTCTAAATGTTGCGGCGGGGCTGCTGGGATTCGCAATCATGAAAGGATACACTGCCTTACTTGACAAGTTAGGTCTGTTGAAGCAGCCTTTCACCCGTCAAGAAAACACAGTGATTCAAACCTGTGTTGTGGCCTCGTCTGGGATAGCCTTTAGCAGCGGCATGGGGAGTTATTTACTAGGGATGAGCCCTCTAGTAGCTGCTCAGGGAGATGCAGGCAACACTCTAATCAACATCAAGAGGCTTGCGATTCCGTGGATGACAGGATATCTCTTCACAGTCAGCTTCGTTGGGCTGTTTTCAATGATACCCTTGAGAAAGATGATGATCATTGATAGAAAACTAACGTACCCAAGTGGAACAGCAACAGCGCACCTCATCAACAGCTTCCACACCCCCAAAGGAGCCAAGAAGGCAGCGAGACAGGTTGCTGTTCTCTTCAAGAGCTGCTGCGGCAGCTTTGCGTGGTCCTTTTTTCAGTGGTTTTTCGCGGCTTCGGATGGCTGTGGGTTTACTAGCTTTCCAATTTTCGGTCTCAAAGCCTACAAACAGAGGTTTTACTTCGACTTCTCAACCACATATATTGGTTGTGGAATGATCACCTCTTACATGGTGAACGTATCCATGCTCGTTGGAGCTGTGATATCATGGGGAATCATGTGGCCTTTGATTGAACAAAAGAAAGGTATCTGGTATGGTGCTCATCTTTCTGCTAGTAGTCTTCATGGAATCCAAGGTTACAGGGTCTTTATTTCCATAGCAATGATACTTGGTGATGGTCTATACCATGTTATGTACCATGCTGGTTATGCGGTTTTCATTATCATAAAAAGTCGGTTCAGCAATAAACCAGAAGAAAAGAAAGTAGAGTTGTCATCGCCACCACCTCTACCATTAACCAATATGGATGCTGGATTGCGCTCATCAGAAACTGAACTGAGTGAAAACTACGACGAGCAAAGAAGAACTGAGTACTTCTTGAAGGACCAAATTCCGCGCCGCTTTGCTGTCTCTGGCTACGTTGCTCTTGCAGCCATATCTTCCGGTGTCCTTCCATTAGTGTTTCACCAACTAAAATGGTATCATGTTGCCCTAGCTTACTCCATCGCTCCGGTTCTGGCCTTCTGCAATGCCTATGGCTGCGGCCTCACAGATTGGTCCCTCGCTTCCAACTACGGCAAAATCGCAATCCTGATCTTCAGTGCTTGTGTTGGCCCTGAAAAAGGTGGTGTCATTGCTGGCCTCGCTTCTTGTGGTGTAATGATGAGCATTGTCTCAACAGCCTCGGATCTCATGCAAGACTTCAAGACTGGATATCTAACTCTCGCGTCTCCTCGATCCATGTTCGTTAGCCAAGTTTTCGGGACAGCCATGGGGTGTGTCATGTCGCCTTTGATATTCTGGTTCTTCTACAAAGCTTACCGAGTTGGCGATCCAACGGGCTCGTATCCTGCACCCTATGGCCTAATGTACCGTGGAATAGCACTCCTCGGAGTTGAGGGCTTTGGCTCACTCCCCAAACACTGCGTTGCACTTGCAGTCTCCTTTTTCGCATTTGCCATTGCCATTAACGTAGTAACCGAGCTTTTGAAGCGTTACGAGACCAAGTACAAGATCTACAGATTTGTTCCAAATGCAATGGCCATGGCAGTCCCGTTCTATCTCGGCTCCTACTTCGTGATTGACATGTGCATAGGAAGCTTGATCCTTTTCGGCTGGAAGTTATGGAACAAACAACAGGCTGAGGATTTAGCACCGGCGGTTGCATCTGGTCTCATTTGTGGCGAGTCTATGTGGGGTGTTCCAGCTGCGATCCTATCTCTTGCCAATGTCAAAGCACCAATATGCATGAAGTTCCTCTCTTACTCAGACAATGATAGAGTTGATTCCTTTTTATCTTCATCAGGATCTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

707

Amino Acids

77.61

Weight (kDa)

9.05

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 38 - 675 2.8e-151 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 94
AccII CGCG 3 cut(s) 698, 1204, 1535
AclWI GGATC 6 cut(s) 1503, 1539, 1634, 1906, 2014, 2122
AcoI YGGCCR 1 cut(s) 1848
AcsI RAATTY 1 cut(s) 1197
AcuI CTGAAG 2 cut(s) 30, 1390
AfaI GTAC 5 cut(s) 557, 1004, 1181, 1679, 1817
AfiI CCNNNNNNNGG 3 cut(s) 348, 737, 1430
AflIII ACRYGT 1 cut(s) 1893
AgsI TTSAA 9 cut(s) 163, 289, 326, 510, 649, 881, 1189, 1513, 1798
AleI CACNNNNGTG 1 cut(s) 92
Alw21I GWGCWC 1 cut(s) 910
Alw26I GTCTC 9 cut(s) 78, 622, 746, 1222, 1486, 1542, 1753, 1802, 1985
AlwI GGATC 6 cut(s) 1503, 1539, 1634, 1906, 2014, 2122
AlwNI CAGNNNCTG 1 cut(s) 1139
AoxI GGCC 8 cut(s) 339, 869, 1326, 1351, 1420, 1447, 1669, 1848
ApoI RAATTY 1 cut(s) 1197
AspLEI GCGC 3 cut(s) 580, 1128, 1206
AspS9I GGNCC 4 cut(s) 675, 1192, 1365, 1421
AsuHPI GGTGA 6 cut(s) 181, 292, 799, 832, 992, 1265
AvaII GGWCC 3 cut(s) 675, 1192, 1365
BaeI ACNNNNGTAYC 2 cut(s) 539, 572
BalI TGGCCA 1 cut(s) 1850
BanI GGYRCC 1 cut(s) 94
BanII GRGCYC 2 cut(s) 398, 1652
BbsI GAAGAC 2 cut(s) 11, 920
Bbv12I GWGCWC 1 cut(s) 910
BbvCI CCTCAGC 1 cut(s) 1950
BccI CCATC 4 cut(s) 214, 701, 977, 1319
BceAI ACGGC 1 cut(s) 1399
BciVI GTATCC 3 cut(s) 248, 838, 1665
BclI TGATCA 3 cut(s) 25, 534, 804
BcoDI GTCTC 9 cut(s) 78, 622, 746, 1222, 1486, 1542, 1753, 1802, 1985
BfaI CTAG 5 cut(s) 386, 401, 723, 921, 1301
BfmI CTRYAG 1 cut(s) 1825
BfuI GTATCC 3 cut(s) 248, 838, 1665
BglI GCCNNNNNGGC 2 cut(s) 103, 620
BglII AGATCT 1 cut(s) 1821
BmcAI AGTACT 1 cut(s) 1181
Bme18I GGWCC 3 cut(s) 675, 1192, 1365
BmgT120I GGNCC 4 cut(s) 675, 1192, 1365, 1421
BmiI GGNNCC 4 cut(s) 96, 613, 1367, 1876
BmsI GCATC 4 cut(s) 162, 409, 1105, 1982
BpiI GAAGAC 2 cut(s) 11, 920
Bpu10I CCTNAGC 2 cut(s) 411, 1950
BpuEI CTTGAG 1 cut(s) 541
BsaBI GATNNNNATC 1 cut(s) 533
BsaI GGTCTC 3 cut(s) 746, 1802, 1985
BsaJI CCNNGG 8 cut(s) 101, 461, 940, 1491, 1578, 1681, 1696, 1851
BsaWI WCCGGW 2 cut(s) 1250, 1318
Bsc4I CCNNNNNNNGG 3 cut(s) 348, 737, 1430
Bse118I RCCGGY 1 cut(s) 1963
Bse1I ACTGG 2 cut(s) 199, 1522
Bse3DI GCAATG 5 cut(s) 975, 1342, 1440, 1767, 1851
Bse8I GATNNNNATC 1 cut(s) 533
BseDI CCNNGG 8 cut(s) 101, 461, 940, 1491, 1578, 1681, 1696, 1851
BseGI GGATG 4 cut(s) 396, 471, 712, 1120
BseJI GATNNNNATC 1 cut(s) 533
BseLI CCNNNNNNNGG 3 cut(s) 348, 737, 1430
BseMI GCAATG 5 cut(s) 975, 1342, 1440, 1767, 1851
BseMII CTCAG 5 cut(s) 425, 1136, 1167, 1941, 2088
BseNI ACTGG 2 cut(s) 199, 1522
BseRI GAGGAG 2 cut(s) 1530, 1685
BseYI CCCAGC 1 cut(s) 232
BsgI GTGCAG 1 cut(s) 1644
Bsh1236I CGCG 3 cut(s) 698, 1204, 1535
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
BshNI GGYRCC 1 cut(s) 94
BsiEI CGRYCG 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 910
BsiSI CCGG 3 cut(s) 1251, 1319, 1964
BslFI GGGAC 3 cut(s) 1351, 1585, 1844
BslI CCNNNNNNNGG 3 cut(s) 348, 737, 1430
BsmAI GTCTC 9 cut(s) 78, 622, 746, 1222, 1486, 1542, 1753, 1802, 1985
BsmBI CGTCTC 1 cut(s) 1542
BsmFI GGGAC 3 cut(s) 1351, 1585, 1844
BsmI GAATGC 1 cut(s) 77
BsnI GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
Bso31I GGTCTC 3 cut(s) 746, 1802, 1985
Bsp1286I GDGCHC 3 cut(s) 398, 910, 1652
Bsp19I CCATGG 3 cut(s) 101, 1578, 1851
BspANI GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
BspCNI CTCAG 5 cut(s) 424, 1137, 1168, 1942, 2087
BspFNI CGCG 3 cut(s) 698, 1204, 1535
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 4 cut(s) 96, 613, 1367, 1876
BspPI GGATC 6 cut(s) 1503, 1539, 1634, 1906, 2014, 2122
BspT107I GGYRCC 1 cut(s) 94
BspTNI GGTCTC 3 cut(s) 746, 1802, 1985
BsrDI GCAATG 5 cut(s) 975, 1342, 1440, 1767, 1851
BsrFI RCCGGY 1 cut(s) 1963
BsrI ACTGG 2 cut(s) 199, 1522
BssAI RCCGGY 1 cut(s) 1963
BssECI CCNNGG 8 cut(s) 101, 461, 940, 1491, 1578, 1681, 1696, 1851
BssT1I CCWWGG 4 cut(s) 101, 940, 1578, 1851
Bst4CI ACNGT 4 cut(s) 121, 319, 487, 1682
Bst6I CTCTTC 2 cut(s) 485, 650
BstC8I GCNNGC 4 cut(s) 179, 424, 453, 1447
BstDEI CTNAG 7 cut(s) 149, 411, 1145, 1176, 1950, 2074, 2118
BstDSI CCRYGG 5 cut(s) 101, 461, 1578, 1681, 1851
BstENI CCTNNNNNAGG 1 cut(s) 1428
BstF5I GGATG 4 cut(s) 396, 471, 712, 1120
BstFNI CGCG 3 cut(s) 698, 1204, 1535
BstHHI GCGC 3 cut(s) 580, 1128, 1206
BstMAI GTCTC 9 cut(s) 78, 622, 746, 1222, 1486, 1542, 1753, 1802, 1985
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 9 cut(s) 103, 620, 660, 663, 1016, 1238, 1451, 1798, 1905
BstNSI RCATGY 1 cut(s) 1897
BstSFI CTRYAG 1 cut(s) 1825
BstUI CGCG 3 cut(s) 698, 1204, 1535
BstV2I GAAGAC 2 cut(s) 11, 920
BstX2I RGATCY 3 cut(s) 1495, 1821, 2114
BstYI RGATCY 3 cut(s) 1495, 1821, 2114
BsuI GTATCC 3 cut(s) 248, 838, 1665
BsuRI GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
BtgI CCRYGG 5 cut(s) 101, 461, 1578, 1681, 1851
BtgZI GCGATG 2 cut(s) 1068, 1297
BtsCI GGATG 4 cut(s) 396, 471, 712, 1120
BtsI GCAGTG 2 cut(s) 258, 1729
BtsIMutI CAGTG 6 cut(s) 192, 258, 324, 692, 1414, 1729
Cac8I GCNNGC 4 cut(s) 179, 424, 453, 1447
CaiI CAGNNNCTG 1 cut(s) 1139
CciI TCATGA 1 cut(s) 246
CfoI GCGC 3 cut(s) 580, 1128, 1206
Cfr10I RCCGGY 1 cut(s) 1963
Cfr13I GGNCC 4 cut(s) 675, 1192, 1365, 1421
CseI GACGC 1 cut(s) 1524
Csp6I GTAC 5 cut(s) 556, 1003, 1180, 1678, 1816
CspCI CAANNNNNGTGG 2 cut(s) 772, 807
CviQI GTAC 5 cut(s) 556, 1003, 1180, 1678, 1816
DdeI CTNAG 7 cut(s) 149, 411, 1145, 1176, 1950, 2074, 2118
EaeI YGGCCR 1 cut(s) 1848
Eam1104I CTCTTC 2 cut(s) 485, 650
EarI CTCTTC 2 cut(s) 485, 650
Eco130I CCWWGG 4 cut(s) 101, 940, 1578, 1851
Eco24I GRGCYC 2 cut(s) 398, 1652
Eco31I GGTCTC 3 cut(s) 746, 1802, 1985
Eco32I GATATC 3 cut(s) 476, 852, 1523
Eco47I GGWCC 3 cut(s) 675, 1192, 1365
Eco57I CTGAAG 2 cut(s) 30, 1390
EcoNI CCTNNNNNAGG 1 cut(s) 1428
EcoRV GATATC 3 cut(s) 476, 852, 1523
EcoT14I CCWWGG 4 cut(s) 101, 940, 1578, 1851
EcoT22I ATGCAT 1 cut(s) 2057
EcoT38I GRGCYC 2 cut(s) 398, 1652
ErhI CCWWGG 4 cut(s) 101, 940, 1578, 1851
Esp3I CGTCTC 1 cut(s) 1542
FaqI GGGAC 3 cut(s) 1351, 1585, 1844
FauI CCCGC 1 cut(s) 217
FbaI TGATCA 3 cut(s) 25, 534, 804
FokI GGATG 4 cut(s) 403, 478, 719, 1127
FriOI GRGCYC 2 cut(s) 398, 1652
FspBI CTAG 5 cut(s) 386, 401, 723, 921, 1301
GlaI GCGC 3 cut(s) 579, 1127, 1205
GsaI CCCAGC 1 cut(s) 236
HaeIII GGCC 8 cut(s) 341, 871, 1328, 1353, 1422, 1449, 1671, 1850
HapII CCGG 3 cut(s) 1251, 1319, 1964
HgaI GACGC 1 cut(s) 1524
HhaI GCGC 3 cut(s) 580, 1128, 1206
Hin6I GCGC 3 cut(s) 578, 1126, 1204
HinP1I GCGC 3 cut(s) 578, 1126, 1204
HindIII AAGCTT 2 cut(s) 1623, 1906
HinfI GANTC 8 cut(s) 62, 237, 322, 457, 861, 936, 1994, 2093
HpaII CCGG 3 cut(s) 1251, 1319, 1964
HphI GGTGA 6 cut(s) 181, 292, 799, 832, 992, 1265
Hpy166II GTNNAC 2 cut(s) 720, 823
Hpy188I TCNGA 6 cut(s) 83, 706, 1135, 1495, 1700, 2077
Hpy8I GTNNAC 2 cut(s) 720, 823
Hpy99I CGWCG 1 cut(s) 1163
HpyAV CCTTC 4 cut(s) 613, 1183, 1268, 1339
HpyCH4III ACNGT 4 cut(s) 121, 319, 487, 1682
HpyCH4IV ACGT 4 cut(s) 554, 825, 1227, 1779
HpyF10VI GCNNNNNNNGC 9 cut(s) 103, 620, 660, 663, 1016, 1238, 1451, 1798, 1905
HpyF3I CTNAG 7 cut(s) 149, 411, 1145, 1176, 1950, 2074, 2118
HpySE526I ACGT 4 cut(s) 554, 825, 1227, 1779
HspAI GCGC 3 cut(s) 578, 1126, 1204
Ksp22I TGATCA 3 cut(s) 25, 534, 804
LmnI GCTCC 4 cut(s) 611, 842, 1321, 1880
LweI GCATC 4 cut(s) 162, 409, 1105, 1982
MaeI CTAG 5 cut(s) 386, 401, 723, 921, 1301
MaeII ACGT 4 cut(s) 554, 825, 1227, 1779
MaeIII GTNAC 4 cut(s) 133, 944, 1783, 1802
MflI RGATCY 3 cut(s) 1495, 1821, 2114
MhlI GDGCHC 3 cut(s) 398, 910, 1652
MlsI TGGCCA 1 cut(s) 1850
MluCI AATT 2 cut(s) 732, 1197
MluNI TGGCCA 1 cut(s) 1850
MlyI GAGTC 2 cut(s) 56, 2003
MmeI TCCRAC 3 cut(s) 820, 1401, 1667
Mox20I TGGCCA 1 cut(s) 1850
Mph1103I ATGCAT 1 cut(s) 2057
MscI TGGCCA 1 cut(s) 1850
MseI TTAA 2 cut(s) 1103, 1776
MslI CAYNNNNRTG 3 cut(s) 92, 968, 2050
Msp20I TGGCCA 1 cut(s) 1850
MspA1I CMGCKG 2 cut(s) 366, 2015
MspI CCGG 3 cut(s) 1251, 1319, 1964
Mva1269I GAATGC 1 cut(s) 77
MvnI CGCG 3 cut(s) 698, 1204, 1535
MwoI GCNNNNNNNGC 9 cut(s) 103, 620, 660, 663, 1016, 1238, 1451, 1798, 1905
NcoI CCATGG 3 cut(s) 101, 1578, 1851
NlaIV GGNNCC 4 cut(s) 96, 613, 1367, 1876
NmeAIII GCCGAG 1 cut(s) 1851
NmuCI GTSAC 1 cut(s) 133
NsiI ATGCAT 1 cut(s) 2057
NspI RCATGY 1 cut(s) 1897
OliI CACNNNNGTG 1 cut(s) 92
PagI TCATGA 1 cut(s) 246
PciI ACATGT 1 cut(s) 1893
PcsI WCGNNNNNNNCGW 1 cut(s) 1785
PctI GAATGC 1 cut(s) 77
PfeI GAWTC 6 cut(s) 237, 322, 457, 861, 936, 2093
PleI GAGTC 2 cut(s) 56, 2002
PpsI GAGTC 2 cut(s) 56, 2002
PscI ACATGT 1 cut(s) 1893
PspFI CCCAGC 1 cut(s) 232
PspN4I GGNNCC 4 cut(s) 96, 613, 1367, 1876
PspPI GGNCC 4 cut(s) 675, 1192, 1365, 1421
PstNI CAGNNNCTG 1 cut(s) 1139
PsuI RGATCY 3 cut(s) 1495, 1821, 2114
PvuII CAGCTG 1 cut(s) 2015
RsaI GTAC 5 cut(s) 557, 1004, 1181, 1679, 1817
RsaNI GTAC 5 cut(s) 556, 1003, 1180, 1678, 1816
RseI CAYNNNNRTG 3 cut(s) 92, 968, 2050
SaqAI TTAA 2 cut(s) 1103, 1776
Sau96I GGNCC 4 cut(s) 675, 1192, 1365, 1421
ScaI AGTACT 1 cut(s) 1181
SchI GAGTC 2 cut(s) 56, 2003
SduI GDGCHC 3 cut(s) 398, 910, 1652
SfaNI GCATC 4 cut(s) 162, 409, 1105, 1982
SfcI CTRYAG 1 cut(s) 1825
SgrAI CRCCGGYG 1 cut(s) 1963
SinI GGWCC 3 cut(s) 675, 1192, 1365
SmiMI CAYNNNNRTG 3 cut(s) 92, 968, 2050
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
Sse9I AATT 2 cut(s) 732, 1197
SspMI CTAG 5 cut(s) 386, 401, 723, 921, 1301
StyI CCWWGG 4 cut(s) 101, 940, 1578, 1851
TaaI ACNGT 4 cut(s) 121, 319, 487, 1682
TaiI ACGT 4 cut(s) 557, 828, 1230, 1782
TaqI TCGA 4 cut(s) 18, 65, 771, 1543
TaqII GACCGA 2 cut(s) 83, 728
TasI AATT 2 cut(s) 732, 1197
TatI WGTACW 2 cut(s) 1179, 1815
TauI GCSGC 6 cut(s) 224, 369, 663, 701, 1209, 1353
TfiI GAWTC 6 cut(s) 237, 322, 457, 861, 936, 2093
Tru1I TTAA 2 cut(s) 1103, 1776
Tru9I TTAA 2 cut(s) 1103, 1776
TscAI CASTG 6 cut(s) 199, 265, 324, 692, 1414, 1736
TseFI GTSAC 1 cut(s) 133
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 7 cut(s) 17, 157, 263, 920, 1015, 2072, 2097
TspGWI ACGGA 2 cut(s) 104, 450
TspRI CASTG 6 cut(s) 199, 265, 324, 692, 1414, 1736
VpaK11BI GGWCC 3 cut(s) 675, 1192, 1365
XagI CCTNNNNNAGG 1 cut(s) 1428
XapI RAATTY 1 cut(s) 1197
XceI RCATGY 1 cut(s) 1897
XspI CTAG 5 cut(s) 386, 401, 723, 921, 1301
ZrmI AGTACT 1 cut(s) 1181
Zsp2I ATGCAT 1 cut(s) 2057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.