RLG00000018751

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001164
Physical Location & Seq
Forward (+)
35339103 .. 35340280
1178 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018751

Sequence Viewer

Length: 1116 bp
ATGCTCGTTGGGGCTGTGATATCATGGGGAATCATGTGGCCTTTGATTGAACAAAAGAAAGGTATCTGGTATGGAGCTCATCTTTCTGCAAGTAGTCTTCATGGAATCCAAGGTTACAGGGTCTTTATCTCCATAGGCATGATACTTGGTGATGGACTATACCATGTTATGTACCATGCTGGTTATATGGGTTTCATTGTCATTAAAAGTTTCTTTAGCAAGAAACCAGAAGAAATGAAAGGAGAGTTGGCATCGCCTTCCCCGCCACCAGTATCATTATCCAAGATGGAAGCTGGATTGCCCTCATCAAAAACTGAGCTGAGTGAAAACTACGACGAGGACAAAGAACTGAGCATATCAATTATTGTCCTGCCATTCATTTTCCACCAACTAAAACTGTATCATGTTGTGACAACTTACATCATCGCTCCGGTTCTGGCCCTCTGCAATGCCTATGGTTGCGGCCTCACAGATTGGTCCCTTGCTTCCAACTACGGCAGAGTCGCAATCCTGATCTTCAGTGCTTGGGTTGGCCCTGAAAAAGGTGGTGTCATTGCTGGCCTCGCTTTTTGTGGTGTAATGATGAGCATTGTCTCGACAGCCTCCGATCTCATGCAAGACTTCAAGACTGGATACCTAACTCTCTCGTCTCCTCGAGCCATGTTCTTCAGCCAAGTTTTCAGGACAGCCATGGGGTGTGTCATGTCACCTTTGATATTCTGGTTCTTCTACAAGGCTTACCGAGTTGAGGATCCCACGGGCTCATATCCTGCACCCTATGACCTAATGTACCGTGGCATTGCACTCCTCAGAGTTGAGGGCTTTGGCTCACTCCCTAAACACTGCGTCACACTTGCAGTCTCCTTTTTCGCATTTGCCATTGCCATTAACATAGTAACCAAGCTTTTGAAGCGTTACGAGACCAAGTACAAGATCTACAGGTTTGTTCCAAACGCAATGGCCATGGCAGTCCCATTCTATCTCGGCTCCTACTTCGTGATTGACATGTGTTTGGGAAGCCTGGTCCTTATCTGCTGGAAGTTACGGAAAAAAAAGCAGGCGGAGGATTTGGCACCGGCGGTTGCATCTGGTCTCATATGTGGCGAGTCTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

372

Amino Acids

41.1

Weight (kDa)

8.84

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 1 - 71 7.7e-13 OPT oligopeptide transporter protein
OPT PF03169 118 - 370 1.4e-60 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1072
AciI CCGC 4 cut(s) 263, 462, 1061, 1079
AclWI GGATC 2 cut(s) 746, 759
AcoI YGGCCR 1 cut(s) 960
AcuI CTGAAG 2 cut(s) 502, 652
AfaI GTAC 3 cut(s) 173, 791, 929
AfiI CCNNNNNNNGG 2 cut(s) 542, 748
AflIII ACRYGT 1 cut(s) 1005
AgsI TTSAA 3 cut(s) 50, 625, 910
AjnI CCWGG 1 cut(s) 1020
AjuI GAANNNNNNNTTGG 2 cut(s) 230, 262
AluBI AGCT 4 cut(s) 77, 293, 319, 904
AluI AGCT 4 cut(s) 77, 293, 319, 904
Alw21I GWGCWC 1 cut(s) 79
Alw26I GTCTC 5 cut(s) 598, 654, 865, 914, 1097
AlwI GGATC 2 cut(s) 746, 759
Ama87I CYCGRG 1 cut(s) 654
AoxI GGCC 6 cut(s) 38, 438, 463, 532, 559, 960
AspS9I GGNCC 4 cut(s) 439, 477, 533, 1024
AsuHPI GGTGA 2 cut(s) 161, 699
AvaI CYCGRG 1 cut(s) 654
AvaII GGWCC 2 cut(s) 477, 1024
BalI TGGCCA 1 cut(s) 962
BamHI GGATCC 1 cut(s) 751
BanI GGYRCC 1 cut(s) 1072
BanII GRGCYC 2 cut(s) 79, 764
BbsI GAAGAC 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 79
BccI CCATC 2 cut(s) 146, 280
BceAI ACGGC 1 cut(s) 511
BciT130I CCWGG 1 cut(s) 1022
BciVI GTATCC 1 cut(s) 626
BcoDI GTCTC 5 cut(s) 598, 654, 865, 914, 1097
BfmI CTRYAG 1 cut(s) 937
BfuI GTATCC 1 cut(s) 626
BglII AGATCT 1 cut(s) 933
BisI GCNGC 1 cut(s) 463
BlsI GCNGC 1 cut(s) 464
Bme1390I CCNGG 1 cut(s) 1022
Bme18I GGWCC 2 cut(s) 477, 1024
BmeT110I CYCGRG 1 cut(s) 654
BmgT120I GGNCC 4 cut(s) 439, 477, 533, 1024
BmiI GGNNCC 4 cut(s) 479, 753, 988, 1074
BmrFI CCNGG 1 cut(s) 1022
BmsI GCATC 2 cut(s) 260, 1094
BpiI GAAGAC 1 cut(s) 89
BsaI GGTCTC 2 cut(s) 914, 1097
BsaJI CCNNGG 5 cut(s) 109, 690, 756, 793, 963
BsaWI WCCGGW 1 cut(s) 430
Bsc4I CCNNNNNNNGG 2 cut(s) 542, 748
Bse118I RCCGGY 1 cut(s) 1075
Bse1I ACTGG 2 cut(s) 269, 634
Bse3DI GCAATG 5 cut(s) 454, 552, 798, 879, 963
BseBI CCWGG 1 cut(s) 1022
BseDI CCNNGG 5 cut(s) 109, 690, 756, 793, 963
BseLI CCNNNNNNNGG 2 cut(s) 542, 748
BseMI GCAATG 5 cut(s) 454, 552, 798, 879, 963
BseMII CTCAG 4 cut(s) 306, 311, 341, 823
BseNI ACTGG 2 cut(s) 269, 634
BseRI GAGGAG 2 cut(s) 642, 797
BsgI GTGCAG 1 cut(s) 756
BshFI GGCC 6 cut(s) 40, 440, 465, 534, 561, 962
BshNI GGYRCC 1 cut(s) 1072
BsiHKAI GWGCWC 1 cut(s) 79
BsiHKCI CYCGRG 1 cut(s) 654
BsiSI CCGG 2 cut(s) 431, 1076
BslFI GGGAC 2 cut(s) 463, 956
BslI CCNNNNNNNGG 2 cut(s) 542, 748
BsmAI GTCTC 5 cut(s) 598, 654, 865, 914, 1097
BsmBI CGTCTC 1 cut(s) 654
BsmFI GGGAC 2 cut(s) 463, 956
BsnI GGCC 6 cut(s) 40, 440, 465, 534, 561, 962
Bso31I GGTCTC 2 cut(s) 914, 1097
BsoBI CYCGRG 1 cut(s) 654
Bsp1286I GDGCHC 2 cut(s) 79, 764
Bsp143I GATC 4 cut(s) 513, 607, 751, 933
Bsp19I CCATGG 2 cut(s) 690, 963
BspACI CCGC 4 cut(s) 263, 462, 1061, 1079
BspANI GGCC 6 cut(s) 40, 440, 465, 534, 561, 962
BspCNI CTCAG 4 cut(s) 307, 312, 342, 822
BspLI GGNNCC 4 cut(s) 479, 753, 988, 1074
BspPI GGATC 2 cut(s) 746, 759
BspT107I GGYRCC 1 cut(s) 1072
BspTNI GGTCTC 2 cut(s) 914, 1097
BsrDI GCAATG 5 cut(s) 454, 552, 798, 879, 963
BsrFI RCCGGY 1 cut(s) 1075
BsrI ACTGG 2 cut(s) 269, 634
BssAI RCCGGY 1 cut(s) 1075
BssECI CCNNGG 5 cut(s) 109, 690, 756, 793, 963
BssMI GATC 4 cut(s) 513, 607, 751, 933
BssT1I CCWWGG 3 cut(s) 109, 690, 963
Bst2UI CCWGG 1 cut(s) 1022
Bst4CI ACNGT 2 cut(s) 399, 794
BstC8I GCNNGC 2 cut(s) 559, 1059
BstDEI CTNAG 4 cut(s) 315, 320, 350, 809
BstDSI CCRYGG 4 cut(s) 690, 756, 793, 963
BstENI CCTNNNNNAGG 1 cut(s) 540
BstKTI GATC 4 cut(s) 516, 610, 754, 936
BstMAI GTCTC 5 cut(s) 598, 654, 865, 914, 1097
BstMBI GATC 4 cut(s) 513, 607, 751, 933
BstMWI GCNNNNNNNGC 3 cut(s) 262, 563, 910
BstNI CCWGG 1 cut(s) 1022
BstNSI RCATGY 1 cut(s) 1009
BstSCI CCNGG 1 cut(s) 1020
BstSFI CTRYAG 1 cut(s) 937
BstV2I GAAGAC 1 cut(s) 89
BstX2I RGATCY 2 cut(s) 751, 933
BstYI RGATCY 2 cut(s) 751, 933
BsuI GTATCC 1 cut(s) 626
BsuRI GGCC 6 cut(s) 40, 440, 465, 534, 561, 962
BtgI CCRYGG 4 cut(s) 690, 756, 793, 963
BtgZI GCGATG 2 cut(s) 237, 409
BtsI GCAGTG 1 cut(s) 841
BtsIMutI CAGTG 2 cut(s) 526, 841
Cac8I GCNNGC 2 cut(s) 559, 1059
Cfr10I RCCGGY 1 cut(s) 1075
Cfr13I GGNCC 4 cut(s) 439, 477, 533, 1024
CseI GACGC 1 cut(s) 835
Csp6I GTAC 3 cut(s) 172, 790, 928
CviQI GTAC 3 cut(s) 172, 790, 928
DdeI CTNAG 4 cut(s) 315, 320, 350, 809
DpnI GATC 4 cut(s) 515, 609, 753, 935
DpnII GATC 4 cut(s) 513, 607, 751, 933
EaeI YGGCCR 1 cut(s) 960
EciI GGCGGA 1 cut(s) 1076
Ecl136II GAGCTC 1 cut(s) 77
Eco130I CCWWGG 3 cut(s) 109, 690, 963
Eco24I GRGCYC 2 cut(s) 79, 764
Eco31I GGTCTC 2 cut(s) 914, 1097
Eco32I GATATC 1 cut(s) 21
Eco47I GGWCC 2 cut(s) 477, 1024
Eco53kI GAGCTC 1 cut(s) 77
Eco57I CTGAAG 2 cut(s) 502, 652
Eco88I CYCGRG 1 cut(s) 654
EcoICRI GAGCTC 1 cut(s) 77
EcoNI CCTNNNNNAGG 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 1020
EcoRV GATATC 1 cut(s) 21
EcoT14I CCWWGG 3 cut(s) 109, 690, 963
EcoT38I GRGCYC 2 cut(s) 79, 764
ErhI CCWWGG 3 cut(s) 109, 690, 963
Esp3I CGTCTC 1 cut(s) 654
FaqI GGGAC 2 cut(s) 463, 956
FauI CCCGC 1 cut(s) 270
FauNDI CATATG 1 cut(s) 1097
Fnu4HI GCNGC 1 cut(s) 463
FriOI GRGCYC 2 cut(s) 79, 764
Fsp4HI GCNGC 1 cut(s) 463
GluI GCNGC 1 cut(s) 463
HaeIII GGCC 6 cut(s) 40, 440, 465, 534, 561, 962
HapII CCGG 2 cut(s) 431, 1076
HgaI GACGC 1 cut(s) 835
HindIII AAGCTT 1 cut(s) 902
HinfI GANTC 4 cut(s) 30, 105, 501, 1106
HpaII CCGG 2 cut(s) 431, 1076
HphI GGTGA 2 cut(s) 161, 699
Hpy188I TCNGA 2 cut(s) 607, 812
Hpy188III TCNNGA 5 cut(s) 511, 595, 625, 682, 997
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 1 cut(s) 267
HpyCH4III ACNGT 2 cut(s) 399, 794
HpyCH4V TGCA 7 cut(s) 89, 447, 616, 773, 803, 857, 1085
HpyF10VI GCNNNNNNNGC 3 cut(s) 262, 563, 910
HpyF3I CTNAG 4 cut(s) 315, 320, 350, 809
Kzo9I GATC 4 cut(s) 513, 607, 751, 933
LmnI GCTCC 3 cut(s) 74, 433, 992
LweI GCATC 2 cut(s) 260, 1094
MaeIII GTNAC 7 cut(s) 113, 409, 705, 847, 895, 914, 1041
MalI GATC 4 cut(s) 515, 609, 753, 935
MboI GATC 4 cut(s) 513, 607, 751, 933
MboII GAAGA 5 cut(s) 89, 242, 508, 658, 718
MflI RGATCY 2 cut(s) 751, 933
MhlI GDGCHC 2 cut(s) 79, 764
MlsI TGGCCA 1 cut(s) 962
MluCI AATT 1 cut(s) 360
MluNI TGGCCA 1 cut(s) 962
MlyI GAGTC 2 cut(s) 510, 1115
MmeI TCCRAC 1 cut(s) 513
Mox20I TGGCCA 1 cut(s) 962
MscI TGGCCA 1 cut(s) 962
MseI TTAA 2 cut(s) 204, 888
MslI CAYNNNNRTG 1 cut(s) 137
Msp20I TGGCCA 1 cut(s) 962
MspI CCGG 2 cut(s) 431, 1076
MspR9I CCNGG 1 cut(s) 1022
MvaI CCWGG 1 cut(s) 1022
MwoI GCNNNNNNNGC 3 cut(s) 262, 563, 910
NcoI CCATGG 2 cut(s) 690, 963
NdeI CATATG 1 cut(s) 1097
NdeII GATC 4 cut(s) 513, 607, 751, 933
NlaIV GGNNCC 4 cut(s) 479, 753, 988, 1074
NmeAIII GCCGAG 1 cut(s) 963
NmuCI GTSAC 3 cut(s) 409, 705, 847
NspI RCATGY 1 cut(s) 1009
PaeR7I CTCGAG 1 cut(s) 654
PciI ACATGT 1 cut(s) 1005
PfeI GAWTC 2 cut(s) 30, 105
PkrI GCNGC 1 cut(s) 464
PleI GAGTC 2 cut(s) 509, 1114
PpsI GAGTC 2 cut(s) 509, 1114
PscI ACATGT 1 cut(s) 1005
Psp124BI GAGCTC 1 cut(s) 79
Psp6I CCWGG 1 cut(s) 1020
PspGI CCWGG 1 cut(s) 1020
PspN4I GGNNCC 4 cut(s) 479, 753, 988, 1074
PspPI GGNCC 4 cut(s) 439, 477, 533, 1024
PspXI VCTCGAGB 1 cut(s) 654
PsuI RGATCY 2 cut(s) 751, 933
RsaI GTAC 3 cut(s) 173, 791, 929
RsaNI GTAC 3 cut(s) 172, 790, 928
RseI CAYNNNNRTG 1 cut(s) 137
SacI GAGCTC 1 cut(s) 79
SaqAI TTAA 2 cut(s) 204, 888
SatI GCNGC 1 cut(s) 463
Sau3AI GATC 4 cut(s) 513, 607, 751, 933
Sau96I GGNCC 4 cut(s) 439, 477, 533, 1024
SchI GAGTC 2 cut(s) 510, 1115
ScrFI CCNGG 1 cut(s) 1022
SduI GDGCHC 2 cut(s) 79, 764
SfaNI GCATC 2 cut(s) 260, 1094
SfcI CTRYAG 1 cut(s) 937
Sfr274I CTCGAG 1 cut(s) 654
SgrAI CRCCGGYG 1 cut(s) 1075
SinI GGWCC 2 cut(s) 477, 1024
SlaI CTCGAG 1 cut(s) 654
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 654
SmoI CTYRAG 1 cut(s) 654
Sse9I AATT 1 cut(s) 360
SsiI CCGC 4 cut(s) 263, 462, 1061, 1079
SstI GAGCTC 1 cut(s) 79
StyD4I CCNGG 1 cut(s) 1020
StyI CCWWGG 3 cut(s) 109, 690, 963
TaaI ACNGT 2 cut(s) 399, 794
TaqI TCGA 2 cut(s) 596, 655
TasI AATT 1 cut(s) 360
TatI WGTACW 1 cut(s) 927
TauI GCSGC 1 cut(s) 465
TfiI GAWTC 2 cut(s) 30, 105
Tru1I TTAA 2 cut(s) 204, 888
Tru9I TTAA 2 cut(s) 204, 888
TscAI CASTG 2 cut(s) 526, 848
TseFI GTSAC 3 cut(s) 409, 705, 847
Tsp45I GTSAC 3 cut(s) 409, 705, 847
TspDTI ATGAA 4 cut(s) 89, 184, 251, 367
TspGWI ACGGA 1 cut(s) 1060
TspRI CASTG 2 cut(s) 526, 848
VpaK11BI GGWCC 2 cut(s) 477, 1024
XagI CCTNNNNNAGG 1 cut(s) 540
XceI RCATGY 1 cut(s) 1009
XhoI CTCGAG 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.