Rh2CG290700

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001164
Physical Location & Seq
Forward (+)
32866660 .. 32868297
1638 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG290700.1

Sequence Viewer

Length: 1638 bp
ATGAAGACTGACGATTTCGACAATGTTCAAGCTGGAAAACAAATGGTTCAGGCTAGAGACTCGACTGAAAAGGCATTCAGAGACACAGAGGTGCCCCCATGGACCATGCAAATAACGGTCAGGTCTATGGTCACAAGCTTTTTTCTTAGCATCATCTTCAACTTCATCGTGTGCAAGCTCAATCTTACCACCGGTGTAATACCATCTCTAAACGTCGCAGCGGGGCTGTTGGGATTCGCACTGCTGAAAGGATACACTGCCTTGCTTCAAAAGTTAGGTCTGTTGAAGCAGCCTTTCACTCGTCAAGAAAACACAGTGGTTCAAACCTGTGTTGTGGCGTCGTCTGGGATAGCCTTCAGCAGTGGCACGGCGAGTTATTTACTAGGTATGAGCCCTCAGGTAGCTGCTCAGGGAGATGCTGGAAACACTCTAATCAACATTAAGAGGCTTTCGATTCCCTGGATGATAGGATATCTCTTCACAGTCTGCTTCGCTGGGCTTTTTTCGATAATAGCCTTGAGAGAGATGATGATCCTCAAAAGAAAATTGACCTACCCAAGTGGAACAGCAACAGCGCACCTCATCAACAACTTCCACACCCCCAAAGGAGCTAGGATGGCAGCGAGACAAGTTTCTGCTCTCTTCAAGAGCTGCTGCTACAGCTTTGTCTGGTCTTTTTTCCAGTGGTTTTTCGCAGCTTCAGATGGCTGTGGGTTTGCTAGCTTTCCAATTTTTGGTCTAAAAGCGTATAAACAAAGGTTTTACTTCGACTTCTCAACCACATATATTGGTTGTGGAATGATCACCTCTTACATGGTGAACATATCCATGCTCGTTGGGGTTGTGATATCATGGGGAATCATGTGGCCTTTGATTGAACAAAAGAAAGGTATCTGGTATGGAGCTCATCTTTCTGCAAGTAGTCTTCATGGAATCCAAGGTTACAGGGTCTTTATCTCCATAGGCATGATACTTGGTGATGGACTATACCATGTTATGTACCATGCTGGTTATATGGGTTTCATTCTCATTAAAAATCTCTTTAGTAATAAACCAGAAGAAAAGAAAGTAGAGTTGGCATCGCCTTTGCCGCCACCACTATCATTATCCAATATGGAAGCTGGCGGATTGCCCTCATCAGAAACTGAGCTGAGTGAAAACTACGACGAGGAACGAAGAACTGAGTACTTCTTGAAAGATCAAATTCCACTCTCGGTTGCTGTCGCTGGCTACATTGCTCTTGCAGGCATATCAATTATTGTCCTGCCATTCATTTTCCACCAACTAAAATGGTATCATGTTCTGACAGCTTACTTAATCGCTCCGGTTCTGGCCTTCTGCAATGCCTATGGCTGCGGCCTCACAGATTGGTCCCTTGCTTCCAACTACGGCAAAATCGCAATCCTGATCTTCAGTGCTTGGGCTGGCCTTGAAAAAGGTGGTGTCATTGCTAGCCTCGCTTCTTGTGGTGCAATGATGAGCATTGTCTCAACAGCCTCCGATCTCATGCAAGACTTCAAGACTGGATACCTAACTCTCTCGTCTCCTCGATTCATGTTCTTCAGCCAAGTTTTCGGGACAGCCATGGGGTGTGTCATGTCGCCTTTGATATTCTGGTTCTTCTACAAAGCTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

545

Amino Acids

60.19

Weight (kDa)

8.93

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 37 - 545 5.2e-119 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 91
AccB7I CCANNNNNTGG 1 cut(s) 734
AciI CCGC 4 cut(s) 221, 1091, 1125, 1356
AclWI GGATC 1 cut(s) 526
AcsI RAATTY 1 cut(s) 1203
AcuI CTGAAG 4 cut(s) 340, 684, 1396, 1546
AcyI GRCGYC 1 cut(s) 338
AfaI GTAC 2 cut(s) 1001, 1187
AfiI CCNNNNNNNGG 1 cut(s) 734
AgeI ACCGGT 1 cut(s) 191
AjnI CCWGG 1 cut(s) 458
AjuI GAANNNNNNNTTGG 2 cut(s) 1058, 1090
AleI CACNNNNGTG 1 cut(s) 89
Alw21I GWGCWC 1 cut(s) 907
Alw26I GTCTC 5 cut(s) 51, 75, 619, 1492, 1548
AlwI GGATC 1 cut(s) 526
AlwNI CAGNNNCTG 1 cut(s) 1145
AoxI GGCC 4 cut(s) 866, 1332, 1357, 1426
ApeKI GCWGC 8 cut(s) 218, 289, 404, 620, 651, 654, 695, 1353
ApoI RAATTY 1 cut(s) 1203
ArsI GACNNNNNNTTYG 2 cut(s) 262, 294
AsiGI ACCGGT 1 cut(s) 191
AspLEI GCGC 1 cut(s) 577
AspS9I GGNCC 2 cut(s) 102, 1371
AsuHPI GGTGA 3 cut(s) 796, 829, 989
AsuNHI GCTAGC 2 cut(s) 719, 1451
AvaII GGWCC 2 cut(s) 102, 1371
AxyI CCTNAGG 1 cut(s) 396
BaeGI GKGCMC 1 cut(s) 96
BanI GGYRCC 1 cut(s) 91
BanII GRGCYC 2 cut(s) 395, 907
BbsI GAAGAC 2 cut(s) 11, 917
Bbv12I GWGCWC 1 cut(s) 907
BbvI GCAGC 8 cut(s) 230, 301, 391, 632, 638, 641, 707, 1340
BccI CCATC 4 cut(s) 211, 610, 698, 974
BceAI ACGGC 2 cut(s) 384, 1405
BciT130I CCWGG 1 cut(s) 460
BciVI GTATCC 2 cut(s) 245, 1520
BclI TGATCA 1 cut(s) 801
BcoDI GTCTC 5 cut(s) 51, 75, 619, 1492, 1548
BfaI CTAG 5 cut(s) 54, 383, 612, 720, 1452
BfmI CTRYAG 1 cut(s) 658
BfuI GTATCC 2 cut(s) 245, 1520
BmcAI AGTACT 1 cut(s) 1187
Bme1390I CCNGG 1 cut(s) 460
Bme18I GGWCC 2 cut(s) 102, 1371
BmgT120I GGNCC 2 cut(s) 102, 1371
BmiI GGNNCC 2 cut(s) 93, 1373
BmrFI CCNGG 1 cut(s) 460
BmsI GCATC 3 cut(s) 159, 406, 1088
BmtI GCTAGC 2 cut(s) 723, 1455
BpiI GAAGAC 2 cut(s) 11, 917
Bpu10I CCTNAGC 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 538
BsaBI GATNNNNATC 1 cut(s) 530
BsaHI GRCGYC 1 cut(s) 338
BsaJI CCNNGG 4 cut(s) 98, 458, 937, 1584
BsaWI WCCGGW 2 cut(s) 191, 1324
Bsc4I CCNNNNNNNGG 1 cut(s) 734
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 2 cut(s) 682, 1528
Bse21I CCTNAGG 1 cut(s) 396
Bse3DI GCAATG 4 cut(s) 1233, 1348, 1446, 1479
Bse8I GATNNNNATC 1 cut(s) 530
BseBI CCWGG 1 cut(s) 460
BseDI CCNNGG 4 cut(s) 98, 458, 937, 1584
BseGI GGATG 2 cut(s) 468, 621
BseJI GATNNNNATC 1 cut(s) 530
BseLI CCNNNNNNNGG 1 cut(s) 734
BseMI GCAATG 4 cut(s) 1233, 1348, 1446, 1479
BseMII CTCAG 5 cut(s) 410, 422, 1137, 1142, 1173
BseNI ACTGG 2 cut(s) 682, 1528
BseRI GAGGAG 1 cut(s) 1536
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 8 cut(s) 230, 301, 391, 632, 638, 641, 707, 1340
BseYI CCCAGC 1 cut(s) 494
BshFI GGCC 4 cut(s) 868, 1334, 1359, 1428
BshNI GGYRCC 1 cut(s) 91
BshTI ACCGGT 1 cut(s) 191
BsiHKAI GWGCWC 1 cut(s) 907
BsiSI CCGG 2 cut(s) 192, 1325
BslFI GGGAC 2 cut(s) 1357, 1591
BslI CCNNNNNNNGG 1 cut(s) 734
BsmAI GTCTC 5 cut(s) 51, 75, 619, 1492, 1548
BsmBI CGTCTC 1 cut(s) 1548
BsmFI GGGAC 2 cut(s) 1357, 1591
BsmI GAATGC 1 cut(s) 74
BsnI GGCC 4 cut(s) 868, 1334, 1359, 1428
Bsp1286I GDGCHC 3 cut(s) 96, 395, 907
Bsp143I GATC 5 cut(s) 531, 801, 1198, 1407, 1501
Bsp19I CCATGG 2 cut(s) 98, 1584
BspACI CCGC 4 cut(s) 221, 1091, 1125, 1356
BspANI GGCC 4 cut(s) 868, 1334, 1359, 1428
BspCNI CTCAG 5 cut(s) 409, 421, 1138, 1143, 1174
BspLI GGNNCC 2 cut(s) 93, 1373
BspOI GCTAGC 2 cut(s) 723, 1455
BspPI GGATC 1 cut(s) 526
BspT107I GGYRCC 1 cut(s) 91
BsrDI GCAATG 4 cut(s) 1233, 1348, 1446, 1479
BsrFI RCCGGY 1 cut(s) 191
BsrI ACTGG 2 cut(s) 682, 1528
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 4 cut(s) 98, 458, 937, 1584
BssMI GATC 5 cut(s) 531, 801, 1198, 1407, 1501
BssNI GRCGYC 1 cut(s) 338
BssT1I CCWWGG 3 cut(s) 98, 937, 1584
Bst2UI CCWGG 1 cut(s) 460
Bst4CI ACNGT 3 cut(s) 118, 316, 484
Bst6I CTCTTC 2 cut(s) 482, 647
BstACI GRCGYC 1 cut(s) 338
BstC8I GCNNGC 7 cut(s) 176, 721, 1123, 1228, 1246, 1426, 1453
BstDEI CTNAG 6 cut(s) 146, 396, 408, 1146, 1151, 1182
BstDSI CCRYGG 2 cut(s) 98, 1584
BstF5I GGATG 2 cut(s) 468, 621
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 5 cut(s) 534, 804, 1201, 1410, 1504
BstMAI GTCTC 5 cut(s) 51, 75, 619, 1492, 1548
BstMBI GATC 5 cut(s) 531, 801, 1198, 1407, 1501
BstMWI GCNNNNNNNGC 4 cut(s) 617, 660, 1090, 1457
BstNI CCWGG 1 cut(s) 460
BstSCI CCNGG 1 cut(s) 458
BstSFI CTRYAG 1 cut(s) 658
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 8 cut(s) 230, 301, 391, 632, 638, 641, 707, 1340
BstV2I GAAGAC 2 cut(s) 11, 917
Bsu36I CCTNAGG 1 cut(s) 396
BsuI GTATCC 2 cut(s) 245, 1520
BsuRI GGCC 4 cut(s) 868, 1334, 1359, 1428
BtgI CCRYGG 2 cut(s) 98, 1584
BtgZI GCGATG 1 cut(s) 1065
BtsCI GGATG 2 cut(s) 468, 621
BtsI GCAGTG 3 cut(s) 239, 255, 367
BtsIMutI CAGTG 6 cut(s) 239, 255, 321, 367, 689, 1420
Cac8I GCNNGC 7 cut(s) 176, 721, 1123, 1228, 1246, 1426, 1453
CaiI CAGNNNCTG 1 cut(s) 1145
CfoI GCGC 1 cut(s) 577
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 2 cut(s) 102, 1371
CseI GACGC 1 cut(s) 327
Csp6I GTAC 2 cut(s) 1000, 1186
CspAI ACCGGT 1 cut(s) 191
CspCI CAANNNNNGTGG 2 cut(s) 769, 804
CviQI GTAC 2 cut(s) 1000, 1186
DdeI CTNAG 6 cut(s) 146, 396, 408, 1146, 1151, 1182
DpnI GATC 5 cut(s) 533, 803, 1200, 1409, 1503
DpnII GATC 5 cut(s) 531, 801, 1198, 1407, 1501
Eam1104I CTCTTC 2 cut(s) 482, 647
EarI CTCTTC 2 cut(s) 482, 647
EciI GGCGGA 1 cut(s) 1140
Ecl136II GAGCTC 1 cut(s) 905
Eco130I CCWWGG 3 cut(s) 98, 937, 1584
Eco24I GRGCYC 2 cut(s) 395, 907
Eco32I GATATC 2 cut(s) 473, 849
Eco47I GGWCC 2 cut(s) 102, 1371
Eco53kI GAGCTC 1 cut(s) 905
Eco57I CTGAAG 4 cut(s) 340, 684, 1396, 1546
Eco81I CCTNAGG 1 cut(s) 396
EcoICRI GAGCTC 1 cut(s) 905
EcoRII CCWGG 1 cut(s) 458
EcoRV GATATC 2 cut(s) 473, 849
EcoT14I CCWWGG 3 cut(s) 98, 937, 1584
EcoT38I GRGCYC 2 cut(s) 395, 907
ErhI CCWWGG 3 cut(s) 98, 937, 1584
Esp3I CGTCTC 1 cut(s) 1548
FaqI GGGAC 2 cut(s) 1357, 1591
FauI CCCGC 1 cut(s) 214
FbaI TGATCA 1 cut(s) 801
FokI GGATG 2 cut(s) 475, 628
FriOI GRGCYC 2 cut(s) 395, 907
FspBI CTAG 5 cut(s) 54, 383, 612, 720, 1452
GlaI GCGC 1 cut(s) 576
GsaI CCCAGC 1 cut(s) 498
HaeIII GGCC 4 cut(s) 868, 1334, 1359, 1428
HapII CCGG 2 cut(s) 192, 1325
HgaI GACGC 1 cut(s) 327
HhaI GCGC 1 cut(s) 577
Hin1I GRCGYC 1 cut(s) 338
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HindIII AAGCTT 2 cut(s) 136, 1629
HinfI GANTC 6 cut(s) 59, 234, 454, 858, 933, 1551
HpaII CCGG 2 cut(s) 192, 1325
HphI GGTGA 3 cut(s) 796, 829, 989
Hpy166II GTNNAC 1 cut(s) 820
Hpy188I TCNGA 5 cut(s) 80, 703, 1141, 1305, 1501
Hpy188III TCNNGA 6 cut(s) 305, 646, 1192, 1405, 1519, 1576
Hpy8I GTNNAC 1 cut(s) 820
Hpy99I CGWCG 3 cut(s) 218, 343, 1169
HpyAV CCTTC 2 cut(s) 364, 1345
HpyCH4III ACNGT 3 cut(s) 118, 316, 484
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 7 cut(s) 109, 174, 917, 1244, 1341, 1472, 1510
HpyF10VI GCNNNNNNNGC 4 cut(s) 617, 660, 1090, 1457
HpyF3I CTNAG 6 cut(s) 146, 396, 408, 1146, 1151, 1182
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 1 cut(s) 338
HspAI GCGC 1 cut(s) 575
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 5 cut(s) 531, 801, 1198, 1407, 1501
LmnI GCTCC 3 cut(s) 608, 902, 1327
Lsp1109I GCAGC 8 cut(s) 230, 301, 391, 632, 638, 641, 707, 1340
LweI GCATC 3 cut(s) 159, 406, 1088
MaeI CTAG 5 cut(s) 54, 383, 612, 720, 1452
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 2 cut(s) 130, 941
MalI GATC 5 cut(s) 533, 803, 1200, 1409, 1503
MboI GATC 5 cut(s) 531, 801, 1198, 1407, 1501
MhlI GDGCHC 3 cut(s) 96, 395, 907
MluCI AATT 4 cut(s) 545, 729, 1203, 1254
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 1 cut(s) 1407
MseI TTAA 3 cut(s) 441, 1032, 1316
MslI CAYNNNNRTG 3 cut(s) 89, 827, 965
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 2 cut(s) 192, 1325
MspR9I CCNGG 1 cut(s) 460
Mva1269I GAATGC 1 cut(s) 74
MvaI CCWGG 1 cut(s) 460
MwoI GCNNNNNNNGC 4 cut(s) 617, 660, 1090, 1457
NcoI CCATGG 2 cut(s) 98, 1584
NdeII GATC 5 cut(s) 531, 801, 1198, 1407, 1501
NheI GCTAGC 2 cut(s) 719, 1451
NlaIV GGNNCC 2 cut(s) 93, 1373
NmuCI GTSAC 1 cut(s) 130
OliI CACNNNNGTG 1 cut(s) 89
PctI GAATGC 1 cut(s) 74
PfeI GAWTC 5 cut(s) 234, 454, 858, 933, 1551
PflMI CCANNNNNTGG 1 cut(s) 734
PinAI ACCGGT 1 cut(s) 191
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
Psp124BI GAGCTC 1 cut(s) 907
Psp6I CCWGG 1 cut(s) 458
PspFI CCCAGC 1 cut(s) 494
PspGI CCWGG 1 cut(s) 458
PspN4I GGNNCC 2 cut(s) 93, 1373
PspPI GGNCC 2 cut(s) 102, 1371
PstNI CAGNNNCTG 1 cut(s) 1145
RsaI GTAC 2 cut(s) 1001, 1187
RsaNI GTAC 2 cut(s) 1000, 1186
RseI CAYNNNNRTG 3 cut(s) 89, 827, 965
SacI GAGCTC 1 cut(s) 907
SaqAI TTAA 3 cut(s) 441, 1032, 1316
Sau3AI GATC 5 cut(s) 531, 801, 1198, 1407, 1501
Sau96I GGNCC 2 cut(s) 102, 1371
ScaI AGTACT 1 cut(s) 1187
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 1 cut(s) 460
SduI GDGCHC 3 cut(s) 96, 395, 907
SfaNI GCATC 3 cut(s) 159, 406, 1088
SfcI CTRYAG 1 cut(s) 658
SgrAI CRCCGGYG 1 cut(s) 191
SinI GGWCC 2 cut(s) 102, 1371
SmiMI CAYNNNNRTG 3 cut(s) 89, 827, 965
SmlI CTYRAG 1 cut(s) 517
SmoI CTYRAG 1 cut(s) 517
Sse9I AATT 4 cut(s) 545, 729, 1203, 1254
SsiI CCGC 4 cut(s) 221, 1091, 1125, 1356
SspMI CTAG 5 cut(s) 54, 383, 612, 720, 1452
SstI GAGCTC 1 cut(s) 907
StyD4I CCNGG 1 cut(s) 458
StyI CCWWGG 3 cut(s) 98, 937, 1584
TaaI ACNGT 3 cut(s) 118, 316, 484
TaiI ACGT 1 cut(s) 216
TaqI TCGA 6 cut(s) 18, 62, 452, 506, 768, 1549
TasI AATT 4 cut(s) 545, 729, 1203, 1254
TatI WGTACW 1 cut(s) 1185
TauI GCSGC 2 cut(s) 1093, 1359
TfiI GAWTC 5 cut(s) 234, 454, 858, 933, 1551
Tru1I TTAA 3 cut(s) 441, 1032, 1316
Tru9I TTAA 3 cut(s) 441, 1032, 1316
TscAI CASTG 6 cut(s) 246, 262, 321, 367, 689, 1420
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 8 cut(s) 218, 289, 404, 620, 651, 654, 695, 1353
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 6 cut(s) 17, 154, 917, 1012, 1261, 1543
TspRI CASTG 6 cut(s) 246, 262, 321, 367, 689, 1420
Van91I CCANNNNNTGG 1 cut(s) 734
VpaK11BI GGWCC 2 cut(s) 102, 1371
XapI RAATTY 1 cut(s) 1203
XspI CTAG 5 cut(s) 54, 383, 612, 720, 1452
ZrmI AGTACT 1 cut(s) 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.