Rh4DG441800

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001164
Physical Location & Seq
Reverse (-)
64321776 .. 64325552
3777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG441800.1

Sequence Viewer

Length: 1833 bp
ATGGGATTATCATCAAGTAAATTAGAGGTTGTTTGTAAAGTTCTTACTATATGTTATTCATCTTATAACTTCAATTTCTTACTTAGGATTGATGTAAATATAGGTGGAACCGCAAGTTATATACTGGGTATGAGTGGAACGATAGCTGCTCAAGGAGATGAAGGCAACACCCCAATCAACGTGAAGAACCCTCACATTGGATGGATGGTTGGATTTCTCTTTACTGTCAGCTTCCTCGGCTTGTTTTCCATTATGCCCATGAGAAAGATAATGATCATGGACTACAAGTTAACCTACCCAAGTGGAACTGCAACTGCATACCTCATTAACAGTTTTCACACCCCCAAAGGAGCCAAGTTAGCAAAGAAACAAGTTGGCGTCCTGTTCAAAAGCTTCTGCTTTAGCTTTGTGTGGGCCTTTTTCCAATGGTTCTATACAGCCGCTGATGGCTGCGGATTTGCTAGCTTCCCAACATTTGGTCTCCAAGCTTATGCGCAGAAGTTTTACTTTGACTTCTCATCGACATACATTGGTGTGGGAATGATTTGCCCTTACATGGTGAACATCTCTTTGCTTGTCGGATCAATTCTTTCATGGGGAATCATGTGGCCTTTGATTGAGAAACATAAAGGTAGCTGGTACAGTGCTGATCTCTCACCGAGCAGTCTCCACGGTATCCAAGGATACAGGGTTTTCATTGCTATAGCCATGATGCTCGGGGATGGTTTATACCATGTAGCTTACATGCTCTTTGTGACCTTCCAAAATTTATGCTGTACACCTAAAACAAAAGAAGAAATGAAATTAGAGTCATCATCCCCACCGTCCATAAACCCTGCAAGTACTGAAGCTGGAGTAGAAGTTTTGAGTGAAAACTATGACGAGGAGCGAAGAGTTGAGTATTTCTTGAAAGACGCAATTTCTCACTGGGTTGCTTTTGGTGGATATGTTGCTCTTGCCATTATATCCATCATTACCGTACCAAGCATTTTCCGCCAATTAAAATGGTATCATATCTTTATTGCTTATTTGGTTGCTCCAGTTTTGGCATTCTGTAATTCCTATGGTGCTGGTCTCACCGATTGGTCCCTCGCCTCCAACTATGGAAAATTCGCCATCATAATCTTCAGTGCTTGGGTTGGCCCTGAAGGTGGTGTTGTTGCTGGGCTTGCTGCTTGTGGTGTCATGATGAGCATTGTCGCTACTGCTTCTGATCTTATGCAGGACTTCAAGACTGGATACCTTACGCTTTCCTCTCCTCGCTCTATGTTCTTCAGCCAAGTTCTTGGCACGGCCATGGGTTGTCTCATGTCCCCTTTGGTATTCTGGATTTTCTACAAAGCTTATCCACTAGGCGATCCAAATGGTTCGTATCCTGCACCGTTTGCACTAATGTACCGTGGAATGGCTCTTTTAGGAGTTGAAGGCGTTGGTTCACTTCCCAAAAATTGTCTGAAACTTGCAGTCTCATTTTTTGTAGCTGCTGTTGTCATAAATTTGATTACTGAGCTTCTAAAGCGTTATGAGACCAAGTACAGGTTGTACAGATTTATTCCTAGCCCAATGTGCATGGCCATCCCATTTTATCTTGGTTCATACTTTGCCATTGACATGTGTTTGGGGAGCTTGATTCTCTTCTTGTGGCAAAGGATGAACAAACAAAAGGCCGATGACTTTGCACCAGCAGTCGCATCTGGACTCATTTGCGGTGAATCTTTGTGGGGTATTCCAGCTGCTATCCTTTCACTGGCAAATGTGAAACCTCCGATCTGTATGAAGTTCCTCTCTGCTTCTGCCAATGAAAAAGTCGAAAAACTTTTATCTGGTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

610

Amino Acids

67.21

Weight (kDa)

8.63

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 32 - 581 8.6e-137 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 66
Acc16I TGCGCA 1 cut(s) 495
AccB7I CCANNNNNTGG 1 cut(s) 476
AciI CCGC 5 cut(s) 111, 441, 453, 994, 1707
AclWI GGATC 2 cut(s) 589, 1352
AcoI YGGCCR 2 cut(s) 1293, 1572
AcsI RAATTY 3 cut(s) 766, 1109, 1495
AcuI CTGAAG 4 cut(s) 867, 1111, 1167, 1258
AcyI GRCGYC 1 cut(s) 378
AfaI GTAC 7 cut(s) 641, 778, 844, 981, 1397, 1535, 1544
AfiI CCNNNNNNNGG 7 cut(s) 197, 476, 556, 1151, 1405, 1536, 1747
AflIII ACRYGT 1 cut(s) 1611
AgsI TTSAA 5 cut(s) 73, 388, 910, 1231, 1424
Alw26I GTCTC 6 cut(s) 485, 671, 1079, 1310, 1471, 1520
AlwI GGATC 2 cut(s) 589, 1352
AlwNI CAGNNNCTG 1 cut(s) 443
Ama87I CYCGRG 1 cut(s) 716
AoxI GGCC 6 cut(s) 414, 608, 1141, 1293, 1572, 1665
ApeKI GCWGC 5 cut(s) 146, 450, 1172, 1481, 1733
ApoI RAATTY 3 cut(s) 766, 1109, 1495
AspLEI GCGC 1 cut(s) 496
AspS9I GGNCC 3 cut(s) 414, 1086, 1142
AsuHPI GGTGA 4 cut(s) 571, 648, 1069, 1721
AsuNHI GCTAGC 1 cut(s) 461
AvaI CYCGRG 1 cut(s) 716
AvaII GGWCC 1 cut(s) 1086
BaeI ACNNNNGTAYC 2 cut(s) 1379, 1412
BalI TGGCCA 1 cut(s) 1574
BbvI GCAGC 5 cut(s) 133, 437, 1159, 1468, 1720
BccI CCATC 7 cut(s) 195, 199, 440, 716, 977, 1124, 1583
BceAI ACGGC 1 cut(s) 1308
BcgI CGANNNNNNTGC 2 cut(s) 1658, 1692
BciVI GTATCC 4 cut(s) 677, 686, 1232, 1383
BclI TGATCA 1 cut(s) 273
BcoDI GTCTC 6 cut(s) 485, 671, 1079, 1310, 1471, 1520
BfaI CTAG 3 cut(s) 462, 1352, 1557
BfmI CTRYAG 1 cut(s) 702
BfuI GTATCC 4 cut(s) 677, 686, 1232, 1383
BisI GCNGC 6 cut(s) 147, 441, 451, 1173, 1482, 1734
BlsI GCNGC 6 cut(s) 148, 442, 452, 1174, 1483, 1735
BmcAI AGTACT 1 cut(s) 844
Bme18I GGWCC 1 cut(s) 1086
BmeT110I CYCGRG 1 cut(s) 716
BmgT120I GGNCC 3 cut(s) 414, 1086, 1142
BmiI GGNNCC 3 cut(s) 109, 352, 1088
BmrI ACTGGG 2 cut(s) 134, 937
BmsI GCATC 2 cut(s) 702, 1700
BmtI GCTAGC 1 cut(s) 465
BmuI ACTGGG 2 cut(s) 134, 937
BpmI CTGGAG 2 cut(s) 873, 1023
BpuEI CTTGAG 1 cut(s) 135
BsaBI GATNNNNATC 2 cut(s) 10, 272
BsaHI GRCGYC 1 cut(s) 378
BsaI GGTCTC 3 cut(s) 485, 1079, 1520
BsaJI CCNNGG 5 cut(s) 235, 670, 679, 1296, 1399
BsaXI ACNNNNNCTCC 2 cut(s) 846, 876
Bsc4I CCNNNNNNNGG 7 cut(s) 197, 476, 556, 1151, 1405, 1536, 1747
Bse1I ACTGG 5 cut(s) 129, 932, 1040, 1240, 1752
Bse3DI GCAATG 1 cut(s) 696
Bse8I GATNNNNATC 2 cut(s) 10, 272
BseDI CCNNGG 5 cut(s) 235, 670, 679, 1296, 1399
BseGI GGATG 6 cut(s) 206, 210, 727, 815, 1575, 1656
BseJI GATNNNNATC 2 cut(s) 10, 272
BseLI CCNNNNNNNGG 7 cut(s) 197, 476, 556, 1151, 1405, 1536, 1747
BseMI GCAATG 1 cut(s) 696
BseMII CTCAG 1 cut(s) 1497
BseNI ACTGG 5 cut(s) 129, 932, 1040, 1240, 1752
BseRI GAGGAG 2 cut(s) 899, 1248
BseXI GCAGC 5 cut(s) 133, 437, 1159, 1468, 1720
BseYI CCCAGC 1 cut(s) 1163
BsgI GTGCAG 1 cut(s) 1362
BshFI GGCC 6 cut(s) 416, 610, 1143, 1295, 1574, 1667
BsiHKCI CYCGRG 1 cut(s) 716
BslFI GGGAC 2 cut(s) 1072, 1297
BslI CCNNNNNNNGG 7 cut(s) 197, 476, 556, 1151, 1405, 1536, 1747
BsmAI GTCTC 6 cut(s) 485, 671, 1079, 1310, 1471, 1520
BsmFI GGGAC 2 cut(s) 1072, 1297
BsmI GAATGC 1 cut(s) 1049
BsnI GGCC 6 cut(s) 416, 610, 1143, 1295, 1574, 1667
Bso31I GGTCTC 3 cut(s) 485, 1079, 1520
BsoBI CYCGRG 1 cut(s) 716
Bsp1407I TGTACA 2 cut(s) 776, 1542
Bsp143I GATC 6 cut(s) 273, 581, 649, 1213, 1357, 1767
Bsp19I CCATGG 1 cut(s) 1296
BspACI CCGC 5 cut(s) 111, 441, 453, 994, 1707
BspANI GGCC 6 cut(s) 416, 610, 1143, 1295, 1574, 1667
BspCNI CTCAG 1 cut(s) 1498
BspHI TCATGA 1 cut(s) 1185
BspLI GGNNCC 3 cut(s) 109, 352, 1088
BspOI GCTAGC 1 cut(s) 465
BspPI GGATC 2 cut(s) 589, 1352
BspTNI GGTCTC 3 cut(s) 485, 1079, 1520
BsrDI GCAATG 1 cut(s) 696
BsrGI TGTACA 2 cut(s) 776, 1542
BsrI ACTGG 5 cut(s) 129, 932, 1040, 1240, 1752
BssECI CCNNGG 5 cut(s) 235, 670, 679, 1296, 1399
BssMI GATC 6 cut(s) 273, 581, 649, 1213, 1357, 1767
BssNI GRCGYC 1 cut(s) 378
BssT1I CCWWGG 2 cut(s) 679, 1296
Bst4CI ACNGT 8 cut(s) 226, 332, 644, 674, 825, 979, 1383, 1400
Bst6I CTCTTC 2 cut(s) 886, 1640
BstACI GRCGYC 1 cut(s) 378
BstAPI GCANNNNNTGC 1 cut(s) 1385
BstAUI TGTACA 2 cut(s) 776, 1542
BstC8I GCNNGC 2 cut(s) 463, 1170
BstDEI CTNAG 2 cut(s) 83, 1506
BstDSI CCRYGG 3 cut(s) 670, 1296, 1399
BstF5I GGATG 6 cut(s) 206, 210, 727, 815, 1575, 1656
BstHHI GCGC 1 cut(s) 496
BstKTI GATC 6 cut(s) 276, 584, 652, 1216, 1360, 1770
BstMAI GTCTC 6 cut(s) 485, 671, 1079, 1310, 1471, 1520
BstMBI GATC 6 cut(s) 273, 581, 649, 1213, 1357, 1767
BstMWI GCNNNNNNNGC 7 cut(s) 237, 359, 993, 1169, 1385, 1516, 1566
BstNSI RCATGY 2 cut(s) 748, 1615
BstSFI CTRYAG 1 cut(s) 702
BstV1I GCAGC 5 cut(s) 133, 437, 1159, 1468, 1720
BstXI CCANNNNNNTGG 1 cut(s) 1286
BsuI GTATCC 4 cut(s) 677, 686, 1232, 1383
BsuRI GGCC 6 cut(s) 416, 610, 1143, 1295, 1574, 1667
BtgI CCRYGG 3 cut(s) 670, 1296, 1399
BtsCI GGATG 6 cut(s) 206, 210, 727, 815, 1575, 1656
BtsIMutI CAGTG 4 cut(s) 649, 925, 1135, 1745
Cac8I GCNNGC 2 cut(s) 463, 1170
CaiI CAGNNNCTG 1 cut(s) 443
CciI TCATGA 1 cut(s) 1185
CfoI GCGC 1 cut(s) 496
Cfr13I GGNCC 3 cut(s) 414, 1086, 1142
CseI GACGC 2 cut(s) 367, 923
Csp6I GTAC 7 cut(s) 640, 777, 843, 980, 1396, 1534, 1543
CviQI GTAC 7 cut(s) 640, 777, 843, 980, 1396, 1534, 1543
DdeI CTNAG 2 cut(s) 83, 1506
DpnI GATC 6 cut(s) 275, 583, 651, 1215, 1359, 1769
DpnII GATC 6 cut(s) 273, 581, 649, 1213, 1357, 1767
EaeI YGGCCR 2 cut(s) 1293, 1572
Eam1104I CTCTTC 2 cut(s) 886, 1640
EarI CTCTTC 2 cut(s) 886, 1640
EciI GGCGGA 1 cut(s) 983
Eco130I CCWWGG 2 cut(s) 679, 1296
Eco31I GGTCTC 3 cut(s) 485, 1079, 1520
Eco47I GGWCC 1 cut(s) 1086
Eco57I CTGAAG 4 cut(s) 867, 1111, 1167, 1258
Eco88I CYCGRG 1 cut(s) 716
EcoT14I CCWWGG 2 cut(s) 679, 1296
ErhI CCWWGG 2 cut(s) 679, 1296
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FaqI GGGAC 2 cut(s) 1072, 1297
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 6 cut(s) 147, 441, 451, 1173, 1482, 1734
FokI GGATG 6 cut(s) 213, 217, 734, 802, 1562, 1663
Fsp4HI GCNGC 6 cut(s) 147, 441, 451, 1173, 1482, 1734
FspBI CTAG 3 cut(s) 462, 1352, 1557
FspI TGCGCA 1 cut(s) 495
GlaI GCGC 1 cut(s) 495
GluI GCNGC 6 cut(s) 147, 441, 451, 1173, 1482, 1734
GsaI CCCAGC 1 cut(s) 1167
GsuI CTGGAG 2 cut(s) 873, 1023
HaeIII GGCC 6 cut(s) 416, 610, 1143, 1295, 1574, 1667
HgaI GACGC 2 cut(s) 367, 923
HhaI GCGC 1 cut(s) 496
Hin1I GRCGYC 1 cut(s) 378
Hin6I GCGC 1 cut(s) 494
HinP1I GCGC 1 cut(s) 494
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HindIII AAGCTT 3 cut(s) 391, 486, 1341
HinfI GANTC 5 cut(s) 600, 809, 1630, 1698, 1712
HpaI GTTAAC 1 cut(s) 291
HphI GGTGA 4 cut(s) 571, 648, 1069, 1721
Hpy166II GTNNAC 4 cut(s) 291, 562, 779, 1436
Hpy188I TCNGA 4 cut(s) 581, 1213, 1455, 1767
Hpy188III TCNNGA 5 cut(s) 907, 1186, 1231, 1327, 1695
Hpy8I GTNNAC 4 cut(s) 291, 562, 779, 1436
HpyAV CCTTC 4 cut(s) 155, 769, 1142, 1418
HpyCH4III ACNGT 8 cut(s) 226, 332, 644, 674, 825, 979, 1383, 1400
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 9 cut(s) 311, 317, 839, 1222, 1379, 1388, 1463, 1569, 1679
HpyF10VI GCNNNNNNNGC 7 cut(s) 237, 359, 993, 1169, 1385, 1516, 1566
HpyF3I CTNAG 2 cut(s) 83, 1506
HpySE526I ACGT 1 cut(s) 180
Hsp92I GRCGYC 1 cut(s) 378
HspAI GCGC 1 cut(s) 494
Ksp22I TGATCA 1 cut(s) 273
KspAI GTTAAC 1 cut(s) 291
Kzo9I GATC 6 cut(s) 273, 581, 649, 1213, 1357, 1767
LmnI GCTCC 4 cut(s) 350, 886, 1042, 1623
Lsp1109I GCAGC 5 cut(s) 133, 437, 1159, 1468, 1720
LweI GCATC 2 cut(s) 702, 1700
MaeI CTAG 3 cut(s) 462, 1352, 1557
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 1 cut(s) 754
MalI GATC 6 cut(s) 275, 583, 651, 1215, 1359, 1769
MboI GATC 6 cut(s) 273, 581, 649, 1213, 1357, 1767
MboII GAAGA 6 cut(s) 196, 806, 903, 1117, 1264, 1627
MlsI TGGCCA 1 cut(s) 1574
MluNI TGGCCA 1 cut(s) 1574
MlyI GAGTC 2 cut(s) 818, 1692
MmeI TCCRAC 3 cut(s) 190, 559, 1122
Mox20I TGGCCA 1 cut(s) 1574
MscI TGGCCA 1 cut(s) 1574
MseI TTAA 3 cut(s) 290, 327, 1001
MslI CAYNNNNRTG 3 cut(s) 533, 1295, 1610
Msp20I TGGCCA 1 cut(s) 1574
MspA1I CMGCKG 2 cut(s) 443, 1733
Mva1269I GAATGC 1 cut(s) 1049
MwoI GCNNNNNNNGC 7 cut(s) 237, 359, 993, 1169, 1385, 1516, 1566
NcoI CCATGG 1 cut(s) 1296
NdeII GATC 6 cut(s) 273, 581, 649, 1213, 1357, 1767
NheI GCTAGC 1 cut(s) 461
NlaIV GGNNCC 3 cut(s) 109, 352, 1088
NmeAIII GCCGAG 1 cut(s) 216
NmuCI GTSAC 1 cut(s) 754
NsbI TGCGCA 1 cut(s) 495
NspI RCATGY 2 cut(s) 748, 1615
PagI TCATGA 1 cut(s) 1185
PciI ACATGT 1 cut(s) 1611
PctI GAATGC 1 cut(s) 1049
PfeI GAWTC 3 cut(s) 600, 1630, 1712
PflMI CCANNNNNTGG 1 cut(s) 476
PkrI GCNGC 6 cut(s) 148, 442, 452, 1174, 1483, 1735
PleI GAGTC 2 cut(s) 817, 1692
PpsI GAGTC 2 cut(s) 817, 1692
PscI ACATGT 1 cut(s) 1611
PsiI TTATAA 1 cut(s) 66
PspFI CCCAGC 1 cut(s) 1163
PspN4I GGNNCC 3 cut(s) 109, 352, 1088
PspPI GGNCC 3 cut(s) 414, 1086, 1142
PstNI CAGNNNCTG 1 cut(s) 443
PvuII CAGCTG 1 cut(s) 1733
RsaI GTAC 7 cut(s) 641, 778, 844, 981, 1397, 1535, 1544
RsaNI GTAC 7 cut(s) 640, 777, 843, 980, 1396, 1534, 1543
RseI CAYNNNNRTG 3 cut(s) 533, 1295, 1610
SaqAI TTAA 3 cut(s) 290, 327, 1001
SatI GCNGC 6 cut(s) 147, 441, 451, 1173, 1482, 1734
Sau3AI GATC 6 cut(s) 273, 581, 649, 1213, 1357, 1767
Sau96I GGNCC 3 cut(s) 414, 1086, 1142
ScaI AGTACT 1 cut(s) 844
SchI GAGTC 2 cut(s) 818, 1692
SfaNI GCATC 2 cut(s) 702, 1700
SfcI CTRYAG 1 cut(s) 702
SinI GGWCC 1 cut(s) 1086
SmiMI CAYNNNNRTG 3 cut(s) 533, 1295, 1610
SmlI CTYRAG 1 cut(s) 150
SmoI CTYRAG 1 cut(s) 150
SsiI CCGC 5 cut(s) 111, 441, 453, 994, 1707
SspMI CTAG 3 cut(s) 462, 1352, 1557
StyI CCWWGG 2 cut(s) 679, 1296
TaaI ACNGT 8 cut(s) 226, 332, 644, 674, 825, 979, 1383, 1400
TaiI ACGT 1 cut(s) 183
TaqI TCGA 2 cut(s) 521, 1809
TatI WGTACW 4 cut(s) 776, 842, 1533, 1542
TauI GCSGC 1 cut(s) 443
TfiI GAWTC 3 cut(s) 600, 1630, 1712
Tru1I TTAA 3 cut(s) 290, 327, 1001
Tru9I TTAA 3 cut(s) 290, 327, 1001
TscAI CASTG 4 cut(s) 649, 932, 1135, 1752
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 5 cut(s) 146, 450, 1172, 1481, 1733
Tsp45I GTSAC 1 cut(s) 754
TspDTI ATGAA 9 cut(s) 48, 174, 582, 685, 815, 1584, 1667, 1790, 1815
TspRI CASTG 4 cut(s) 649, 932, 1135, 1752
Van91I CCANNNNNTGG 1 cut(s) 476
VpaK11BI GGWCC 1 cut(s) 1086
XapI RAATTY 3 cut(s) 766, 1109, 1495
XceI RCATGY 2 cut(s) 748, 1615
XspI CTAG 3 cut(s) 462, 1352, 1557
ZrmI AGTACT 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.