pycom13g00560

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001164
Physical Location & Seq
Forward (+)
353831 .. 355262
1432 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g00560.2

Sequence Viewer

Length: 1287 bp
ATGATTTGCCCTATCATGGTCAACGTATCTTTGCTTGTTGGAGCCATTATTTCATGGGGAATCATGTGGCCCTTGATTGAGACAAAGAAAGGTATCTGGTACAACGCTAATCTATCCGCCAGCAGTCTCCATGGCATCCAAGGATACAGGGTTTTTATTGCTATAGCCATGATGCTTGGTGACGGTTTGTTCCATGTAGTCTACATGCTCTTCATGACCACGAAAAGTCTCATGACACACAACTCAGAGAAGAAACTAGAGACATCATCACCATCTGTAAACCATGACAGTGTTGAAGCGGGAGGGTCCTCAGAAGTTGTATCTGCAGACTATGGTGAAGAACGGAGAATCGAGTACTTCTTGAAAGACCAAATCCCCAGCTGGGTTGCTTTCTCTGGCTACATTGTGCTTGCAGCCATATCCATCGGTGTAGTACCCTTGATCTTCCCCCAGCTGAAATGGTACCATGTGTTGGTTGCTTATCTGATTGCCCCAGTCTTGGCCTTCTGCAATGCCTACGGCTGTGGCCTCACCGATTGGTCTCTTGCCTCCAACTATGGCAAATTCGCCATCATAATCTTCAGCTCTTGGGTTGGCCTTGACCGTGGTGGTGTCATTGCCGGCCTTGCTTCTTGCGGTGTGATGATGAGTATTGTTTCAACCGCTTCTGATCTCATGCAAGACTTCAAGACAGGATACCTCACGCTGTCGTCTCCTCGCTCTATGTTCTTCAGCCAAGTCTTTGGCACAGCAATGGGATGCGTCATGTCTCCATTAATATTCTGGTTTTTCTACAAAGCTTACTCAATTGGCAATCCAGACGGCCCTTATCCTGCACCCTACGGCCTAATGTACCGCGGCATTGCACTCCTTGGAGTTGAGGGTGTCGGTTCCCTCCCCAAAAACTGCGTGACGCTTGCAGTCTCGTTTTTTGCAGCTGCAGTCGCGATGAACATAATCAATGAGCTATTGCAGCGTTACGAAACAAAGTACAGGATCCATAGGTTCATTCCGAACCCAATGTGCATGGCCATCCCATTCTACCTGGGTTCCTACTTCGCCATTGACATGTGCGTGGGAAGCTTGATCCTCTACTTGTGGCGGAGGAAGAACAGGCAAAAGGCCAATGACTACGGGCCAGCTGTGGCGTCGGGTCTCATCTGCGGTGAATCTTTGTGGGGCGTTCCGGCTGCTATACTGTCCCTTGCGTCTGTCAAACCTCCTATCTGCATGAAGTTCCTCTCTGCTTCTGTCAACAAGAAAGTTGATTCCCTATTACGTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

429

Amino Acids

46.94

Weight (kDa)

8.78

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 462
AccB1I GGYRCC 1 cut(s) 462
AccB7I CCANNNNNTGG 1 cut(s) 472
AccI GTMKAC 1 cut(s) 201
AccII CGCG 2 cut(s) 858, 947
AciI CCGC 8 cut(s) 117, 299, 636, 663, 856, 858, 1102, 1164
AclWI GGATC 3 cut(s) 991, 1004, 1081
AcoI YGGCCR 1 cut(s) 1029
AcsI RAATTY 1 cut(s) 563
AcuI CTGAAG 2 cut(s) 565, 715
AcyI GRCGYC 1 cut(s) 1148
AfaI GTAC 6 cut(s) 101, 356, 435, 464, 854, 992
AfiI CCNNNNNNNGG 4 cut(s) 16, 382, 472, 499
AflIII ACRYGT 1 cut(s) 1068
AgsI TTSAA 4 cut(s) 296, 364, 660, 688
AjnI CCWGG 1 cut(s) 1044
AluBI AGCT 9 cut(s) 381, 454, 585, 800, 938, 967, 1083, 1142, 1284
AluI AGCT 9 cut(s) 381, 454, 585, 800, 938, 967, 1083, 1142, 1284
Alw26I GTCTC 9 cut(s) 74, 131, 233, 254, 546, 717, 774, 928, 1160
AlwI GGATC 3 cut(s) 991, 1004, 1081
ApeKI GCWGC 5 cut(s) 413, 935, 938, 973, 1190
ApoI RAATTY 1 cut(s) 563
AseI ATTAAT 1 cut(s) 776
Asp718I GGTACC 1 cut(s) 462
AspS9I GGNCC 4 cut(s) 69, 306, 824, 1136
AsuHPI GGTGA 5 cut(s) 191, 261, 347, 523, 1178
AvaII GGWCC 1 cut(s) 306
BalI TGGCCA 1 cut(s) 1031
BamHI GGATCC 1 cut(s) 996
BanI GGYRCC 1 cut(s) 462
BbvI GCAGC 5 cut(s) 425, 925, 947, 985, 1177
BccI CCATC 4 cut(s) 280, 431, 578, 1040
BceAI ACGGC 3 cut(s) 535, 838, 859
BciT130I CCWGG 1 cut(s) 1046
BciVI GTATCC 2 cut(s) 137, 689
BcoDI GTCTC 9 cut(s) 74, 131, 233, 254, 546, 717, 774, 928, 1160
BfaI CTAG 2 cut(s) 257, 1285
BfmI CTRYAG 3 cut(s) 162, 324, 939
BfuI GTATCC 2 cut(s) 137, 689
BisI GCNGC 6 cut(s) 414, 859, 936, 939, 974, 1191
BlsI GCNGC 6 cut(s) 415, 860, 937, 940, 975, 1192
BmcAI AGTACT 1 cut(s) 356
Bme1390I CCNGG 1 cut(s) 1046
Bme18I GGWCC 1 cut(s) 306
BmgT120I GGNCC 4 cut(s) 69, 306, 824, 1136
BmiI GGNNCC 6 cut(s) 43, 307, 464, 892, 998, 1051
BmrFI CCNGG 1 cut(s) 1046
BmrI ACTGGG 1 cut(s) 488
BmsI GCATC 3 cut(s) 144, 162, 749
BmuI ACTGGG 1 cut(s) 488
BsaAI YACGTR 1 cut(s) 1280
BsaHI GRCGYC 1 cut(s) 1148
BsaI GGTCTC 2 cut(s) 546, 1160
BsaJI CCNNGG 6 cut(s) 130, 139, 604, 856, 871, 1045
Bsc4I CCNNNNNNNGG 4 cut(s) 16, 382, 472, 499
Bse118I RCCGGY 1 cut(s) 620
Bse1I ACTGG 1 cut(s) 494
Bse3DI GCAATG 4 cut(s) 517, 615, 759, 861
BseBI CCWGG 1 cut(s) 1046
BseDI CCNNGG 6 cut(s) 130, 139, 604, 856, 871, 1045
BseGI GGATG 3 cut(s) 135, 764, 1032
BseLI CCNNNNNNNGG 4 cut(s) 16, 382, 472, 499
BseMI GCAATG 4 cut(s) 517, 615, 759, 861
BseMII CTCAG 2 cut(s) 258, 324
BseNI ACTGG 1 cut(s) 494
BseRI GAGGAG 1 cut(s) 705
BseXI GCAGC 5 cut(s) 425, 925, 947, 985, 1177
BseYI CCCAGC 3 cut(s) 377, 381, 450
BsgI GTGCAG 1 cut(s) 819
Bsh1236I CGCG 2 cut(s) 858, 947
BshNI GGYRCC 1 cut(s) 462
BsiSI CCGG 2 cut(s) 621, 1187
BslFI GGGAC 1 cut(s) 1186
BslI CCNNNNNNNGG 4 cut(s) 16, 382, 472, 499
BsmAI GTCTC 9 cut(s) 74, 131, 233, 254, 546, 717, 774, 928, 1160
BsmBI CGTCTC 1 cut(s) 717
BsmFI GGGAC 1 cut(s) 1186
Bso31I GGTCTC 2 cut(s) 546, 1160
Bsp143I GATC 4 cut(s) 441, 670, 996, 1086
Bsp19I CCATGG 1 cut(s) 130
Bsp68I TCGCGA 1 cut(s) 947
BspACI CCGC 8 cut(s) 117, 299, 636, 663, 856, 858, 1102, 1164
BspCNI CTCAG 2 cut(s) 257, 323
BspFNI CGCG 2 cut(s) 858, 947
BspHI TCATGA 2 cut(s) 213, 231
BspLI GGNNCC 6 cut(s) 43, 307, 464, 892, 998, 1051
BspMAI CTGCAG 2 cut(s) 328, 943
BspPI GGATC 3 cut(s) 991, 1004, 1081
BspQI GCTCTTC 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 462
BspTNI GGTCTC 2 cut(s) 546, 1160
BsrDI GCAATG 4 cut(s) 517, 615, 759, 861
BsrFI RCCGGY 1 cut(s) 620
BsrI ACTGG 1 cut(s) 494
BssAI RCCGGY 1 cut(s) 620
BssECI CCNNGG 6 cut(s) 130, 139, 604, 856, 871, 1045
BssMI GATC 4 cut(s) 441, 670, 996, 1086
BssNI GRCGYC 1 cut(s) 1148
BssT1I CCWWGG 3 cut(s) 130, 139, 871
Bst2UI CCWGG 1 cut(s) 1046
Bst4CI ACNGT 4 cut(s) 185, 290, 605, 1200
Bst6I CTCTTC 1 cut(s) 215
BstACI GRCGYC 1 cut(s) 1148
BstBAI YACGTR 1 cut(s) 1280
BstC8I GCNNGC 5 cut(s) 121, 411, 622, 918, 1140
BstDEI CTNAG 2 cut(s) 244, 310
BstDSI CCRYGG 3 cut(s) 130, 604, 856
BstF5I GGATG 3 cut(s) 135, 764, 1032
BstFNI CGCG 2 cut(s) 858, 947
BstKTI GATC 4 cut(s) 444, 673, 999, 1089
BstMAI GTCTC 9 cut(s) 74, 131, 233, 254, 546, 717, 774, 928, 1160
BstMBI GATC 4 cut(s) 441, 670, 996, 1086
BstMWI GCNNNNNNNGC 4 cut(s) 626, 944, 973, 1080
BstNI CCWGG 1 cut(s) 1046
BstNSI RCATGY 2 cut(s) 208, 1072
BstSCI CCNGG 1 cut(s) 1044
BstSFI CTRYAG 3 cut(s) 162, 324, 939
BstSNI TACGTA 1 cut(s) 1280
BstUI CGCG 2 cut(s) 858, 947
BstV1I GCAGC 5 cut(s) 425, 925, 947, 985, 1177
BstX2I RGATCY 1 cut(s) 996
BstXI CCANNNNNNTGG 1 cut(s) 743
BstYI RGATCY 1 cut(s) 996
BsuI GTATCC 2 cut(s) 137, 689
BtgI CCRYGG 3 cut(s) 130, 604, 856
BtgZI GCGATG 1 cut(s) 962
BtsCI GGATG 3 cut(s) 135, 764, 1032
BtsIMutI CAGTG 1 cut(s) 295
BtuMI TCGCGA 1 cut(s) 947
Cac8I GCNNGC 5 cut(s) 121, 411, 622, 918, 1140
CciI TCATGA 2 cut(s) 213, 231
Cfr10I RCCGGY 1 cut(s) 620
Cfr13I GGNCC 4 cut(s) 69, 306, 824, 1136
Cfr42I CCGCGG 1 cut(s) 859
CseI GACGC 4 cut(s) 751, 922, 1137, 1197
Csp6I GTAC 6 cut(s) 100, 355, 434, 463, 853, 991
CviQI GTAC 6 cut(s) 100, 355, 434, 463, 853, 991
DdeI CTNAG 2 cut(s) 244, 310
DpnI GATC 4 cut(s) 443, 672, 998, 1088
DpnII GATC 4 cut(s) 441, 670, 996, 1086
EaeI YGGCCR 1 cut(s) 1029
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
EciI GGCGGA 2 cut(s) 106, 1117
Eco105I TACGTA 1 cut(s) 1280
Eco130I CCWWGG 3 cut(s) 130, 139, 871
Eco31I GGTCTC 2 cut(s) 546, 1160
Eco47I GGWCC 1 cut(s) 306
Eco57I CTGAAG 2 cut(s) 565, 715
EcoO109I RGGNCCY 1 cut(s) 306
EcoRII CCWGG 1 cut(s) 1044
EcoT14I CCWWGG 3 cut(s) 130, 139, 871
ErhI CCWWGG 3 cut(s) 130, 139, 871
Esp3I CGTCTC 1 cut(s) 717
FaqI GGGAC 1 cut(s) 1186
FauI CCCGC 1 cut(s) 292
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 6 cut(s) 414, 859, 936, 939, 974, 1191
FokI GGATG 3 cut(s) 122, 771, 1019
Fsp4HI GCNGC 6 cut(s) 414, 859, 936, 939, 974, 1191
FspBI CTAG 2 cut(s) 257, 1285
GluI GCNGC 6 cut(s) 414, 859, 936, 939, 974, 1191
GsaI CCCAGC 3 cut(s) 381, 385, 454
HapII CCGG 2 cut(s) 621, 1187
HgaI GACGC 4 cut(s) 751, 922, 1137, 1197
Hin1I GRCGYC 1 cut(s) 1148
HincII GTYRAC 2 cut(s) 22, 1255
HindII GTYRAC 2 cut(s) 22, 1255
HindIII AAGCTT 2 cut(s) 798, 1081
HinfI GANTC 4 cut(s) 60, 348, 1169, 1268
HpaII CCGG 2 cut(s) 621, 1187
HphI GGTGA 5 cut(s) 191, 261, 347, 523, 1178
Hpy166II GTNNAC 4 cut(s) 22, 202, 280, 1255
Hpy188I TCNGA 5 cut(s) 247, 313, 486, 670, 1014
Hpy188III TCNNGA 6 cut(s) 214, 232, 361, 688, 818, 946
Hpy8I GTNNAC 4 cut(s) 22, 202, 280, 1255
Hpy99I CGWCG 1 cut(s) 1153
HpyAV CCTTC 1 cut(s) 514
HpyCH4III ACNGT 4 cut(s) 185, 290, 605, 1200
HpyCH4IV ACGT 2 cut(s) 24, 1279
HpyF10VI GCNNNNNNNGC 4 cut(s) 626, 944, 973, 1080
HpyF3I CTNAG 2 cut(s) 244, 310
HpySE526I ACGT 2 cut(s) 24, 1279
Hsp92I GRCGYC 1 cut(s) 1148
KpnI GGTACC 1 cut(s) 466
KroI GCCGGC 1 cut(s) 620
KroNI GCCGGC 1 cut(s) 622
KspI CCGCGG 1 cut(s) 859
Kzo9I GATC 4 cut(s) 441, 670, 996, 1086
LguI GCTCTTC 1 cut(s) 215
LmnI GCTCC 1 cut(s) 41
Lsp1109I GCAGC 5 cut(s) 425, 925, 947, 985, 1177
LweI GCATC 3 cut(s) 144, 162, 749
MaeI CTAG 2 cut(s) 257, 1285
MaeII ACGT 2 cut(s) 24, 1279
MaeIII GTNAC 3 cut(s) 179, 910, 977
MalI GATC 4 cut(s) 443, 672, 998, 1088
MboI GATC 4 cut(s) 441, 670, 996, 1086
MboII GAAGA 7 cut(s) 202, 262, 350, 436, 571, 721, 1120
MfeI CAATTG 1 cut(s) 807
MflI RGATCY 1 cut(s) 996
MlsI TGGCCA 1 cut(s) 1031
MluCI AATT 2 cut(s) 563, 807
MluNI TGGCCA 1 cut(s) 1031
MmeI TCCRAC 2 cut(s) 19, 576
Mox20I TGGCCA 1 cut(s) 1031
MroNI GCCGGC 1 cut(s) 620
MscI TGGCCA 1 cut(s) 1031
MseI TTAA 1 cut(s) 776
MslI CAYNNNNRTG 4 cut(s) 288, 752, 1067, 1073
Msp20I TGGCCA 1 cut(s) 1031
MspA1I CMGCKG 5 cut(s) 381, 454, 858, 938, 1142
MspI CCGG 2 cut(s) 621, 1187
MspR9I CCNGG 1 cut(s) 1046
MunI CAATTG 1 cut(s) 807
MvaI CCWGG 1 cut(s) 1046
MvnI CGCG 2 cut(s) 858, 947
MwoI GCNNNNNNNGC 4 cut(s) 626, 944, 973, 1080
NaeI GCCGGC 1 cut(s) 622
NcoI CCATGG 1 cut(s) 130
NdeII GATC 4 cut(s) 441, 670, 996, 1086
NgoMIV GCCGGC 1 cut(s) 620
NlaIV GGNNCC 6 cut(s) 43, 307, 464, 892, 998, 1051
NmuCI GTSAC 2 cut(s) 179, 910
NruI TCGCGA 1 cut(s) 947
NspI RCATGY 2 cut(s) 208, 1072
PagI TCATGA 2 cut(s) 213, 231
PciI ACATGT 1 cut(s) 1068
PciSI GCTCTTC 1 cut(s) 215
PdiI GCCGGC 1 cut(s) 622
PfeI GAWTC 4 cut(s) 60, 348, 1169, 1268
PflMI CCANNNNNTGG 1 cut(s) 472
PkrI GCNGC 6 cut(s) 415, 860, 937, 940, 975, 1192
Ppu21I YACGTR 1 cut(s) 1280
PpuMI RGGWCCY 1 cut(s) 306
PscI ACATGT 1 cut(s) 1068
PshBI ATTAAT 1 cut(s) 776
Psp5II RGGWCCY 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 1044
PspFI CCCAGC 3 cut(s) 377, 381, 450
PspGI CCWGG 1 cut(s) 1044
PspN4I GGNNCC 6 cut(s) 43, 307, 464, 892, 998, 1051
PspPI GGNCC 4 cut(s) 69, 306, 824, 1136
PspPPI RGGWCCY 1 cut(s) 306
PstI CTGCAG 2 cut(s) 328, 943
PsuI RGATCY 1 cut(s) 996
PvuII CAGCTG 4 cut(s) 381, 454, 938, 1142
RruI TCGCGA 1 cut(s) 947
RsaI GTAC 6 cut(s) 101, 356, 435, 464, 854, 992
RsaNI GTAC 6 cut(s) 100, 355, 434, 463, 853, 991
RseI CAYNNNNRTG 4 cut(s) 288, 752, 1067, 1073
SacII CCGCGG 1 cut(s) 859
SapI GCTCTTC 1 cut(s) 215
SaqAI TTAA 1 cut(s) 776
SatI GCNGC 6 cut(s) 414, 859, 936, 939, 974, 1191
Sau3AI GATC 4 cut(s) 441, 670, 996, 1086
Sau96I GGNCC 4 cut(s) 69, 306, 824, 1136
ScaI AGTACT 1 cut(s) 356
ScrFI CCNGG 1 cut(s) 1046
SfaNI GCATC 3 cut(s) 144, 162, 749
SfcI CTRYAG 3 cut(s) 162, 324, 939
Sfr303I CCGCGG 1 cut(s) 859
SgrBI CCGCGG 1 cut(s) 859
SinI GGWCC 1 cut(s) 306
SmiMI CAYNNNNRTG 4 cut(s) 288, 752, 1067, 1073
SnaBI TACGTA 1 cut(s) 1280
Sse9I AATT 2 cut(s) 563, 807
SsiI CCGC 8 cut(s) 117, 299, 636, 663, 856, 858, 1102, 1164
SspI AATATT 1 cut(s) 780
SspMI CTAG 2 cut(s) 257, 1285
StyD4I CCNGG 1 cut(s) 1044
StyI CCWWGG 3 cut(s) 130, 139, 871
TaaI ACNGT 4 cut(s) 185, 290, 605, 1200
TaiI ACGT 2 cut(s) 27, 1282
TaqI TCGA 1 cut(s) 351
TasI AATT 2 cut(s) 563, 807
TatI WGTACW 2 cut(s) 354, 990
TauI GCSGC 1 cut(s) 861
TfiI GAWTC 4 cut(s) 60, 348, 1169, 1268
Tru1I TTAA 1 cut(s) 776
Tru9I TTAA 1 cut(s) 776
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 2 cut(s) 179, 910
TseI GCWGC 5 cut(s) 413, 935, 938, 973, 1190
Tsp45I GTSAC 2 cut(s) 179, 910
TspDTI ATGAA 5 cut(s) 42, 202, 965, 997, 1247
TspGWI ACGGA 1 cut(s) 358
TspRI CASTG 1 cut(s) 295
Van91I CCANNNNNTGG 1 cut(s) 472
VpaK11BI GGWCC 1 cut(s) 306
VspI ATTAAT 1 cut(s) 776
XapI RAATTY 1 cut(s) 563
XceI RCATGY 2 cut(s) 208, 1072
XcmI CCANNNNNNNNNTGG 1 cut(s) 780
XmiI GTMKAC 1 cut(s) 201
XspI CTAG 2 cut(s) 257, 1285
ZrmI AGTACT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.