Rw6G030860

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001164
Physical Location & Seq
Forward (+)
54913804 .. 54917318
3515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G030860.1

Sequence Viewer

Length: 1353 bp
ATGATCAAGCTGAAAAACAAATGGCTCAGGCTAAGGACTCTGATTGAAAAGGCATTAAGAGATAGGGAGGTGCCGCCATGGACCAAGCAAATAACTATGAGGTCTATGGTCGCAAGCTTTTTTCTTAGCATCATTTTCCACTTTCTCATGTGCAAGCTTAATCTTACCACTAATGTCATATGCAAGGTTCGAAAAATCCTACTATCTTGGCTGCTCGGGTTTGGGCTTGGGCTTGGAAAGTGGCTTATGGATAAGGAATTAACTGACTCCTATTTGTTTGTTGGGAAGTGGCTCTCTGTTGGTACAGCAATTGCGCGCCTGGCTAATGGGGATGCTAATCAATGTGATTCGACCCTAGCTAGCTACACCCTTGGCTGTGGGTTTGCTAGCTTTCCAATTTTTGGTCAAAGCCTGCAAACAGCTGTGATATCATGGGGAATCATGTGGTCTTTGATTGAACAAAAGAAAGGTATCTGGTACAGTGCTCATCTTTCTGCAAGTAGTCTTCATGGAATCCAAGGTTACAGGTACTTTTTGAAAGACCAAATTCCGAGCTGGATTGCTGCTGCTGGCTACATTGCTCTTGCAATCATATCAATTATTGTCCTGCCATTCATGTTCCACCAACTAAAATGGTATCACGTTGCGAGAGCTTACATCATCGCTCTGGTTCTAGCCTTCTGTAGTGCCTATGGTTGCGGCCTCACAGATTGGTCTCTTGCTTCCTACCACGGCAAAATCGCAATTCTCATCTTCATCATTGCTGGCCTTGCTTCCTGTGGTGTAATGATGAGCATTGTCTCAACAGCTAGCCTCGGATCTCATGCAAGACTTCAAGTCCGGATACCTAACTCTCTCTTCTCCTCTATCCATGTTCCTTACCGAGTTGGTGATTCAACAGGCTCATATCCTGCACCCTATGGCCTAATGTACCGCGGTATTGCACTCCTCGGAGTTGAGGGCATCGGCTCACTCCCCAAACACTGCGCCACGCTTGCAGTCTCATTTTTTGCATTTGCCATTGCCATTAACATAGTAACCGAGATTTTGAAGCGTTACGAGACCAAGTACAAGATCTACAGATTTGTTCCGATCGCAATGGCCATGGCAGTCCCGTTCTATCTCGGCTCCTACTTCATGATTGACAACAAAAAACAAGCTGAGGATTTAGCACCGGCGGTTGCGTCTGGTCTCATCTGTGGTGAGTCTATATGGGGTGTTCCAGCGGCGATCCTATCTCTTGCCAATGTCAAAGCACCGATATGCATGAAGTTCCTCTCTTACTCGGACAACGATGGAGTTGATTCATTTTTATCTTCATCAGGATCTAGGCAGTGTTCAAGTGGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

450

Amino Acids

49.55

Weight (kDa)

9.41

Isoelectric Point (pI)

30.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 179 - 269 6.4e-14 OPT oligopeptide transporter protein
OPT PF03169 295 - 412 8.7e-12 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 70
AccB7I CCANNNNNTGG 1 cut(s) 401
AccII CGCG 2 cut(s) 316, 936
AccIII TCCGGA 1 cut(s) 840
AciI CCGC 6 cut(s) 74, 699, 934, 936, 1178, 1226
AclWI GGATC 3 cut(s) 826, 1225, 1333
AcoI YGGCCR 1 cut(s) 1101
AcsI RAATTY 1 cut(s) 546
AfaI GTAC 5 cut(s) 304, 479, 530, 932, 1070
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 7 cut(s) 47, 458, 538, 836, 897, 1051, 1341
AhdI GACNNNNNGTC 1 cut(s) 836
AjnI CCWGG 1 cut(s) 318
Alw21I GWGCWC 1 cut(s) 487
Alw26I GTCTC 5 cut(s) 720, 805, 1006, 1055, 1196
AlwI GGATC 3 cut(s) 826, 1225, 1333
Ama87I CYCGRG 1 cut(s) 215
Aor13HI TCCGGA 1 cut(s) 840
AoxI GGCC 4 cut(s) 700, 766, 922, 1101
ApeKI GCWGC 3 cut(s) 211, 563, 566
ApoI RAATTY 1 cut(s) 546
AspLEI GCGC 3 cut(s) 316, 318, 989
AspS9I GGNCC 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 902, 1214
AsuII TTCGAA 1 cut(s) 190
AsuNHI GCTAGC 3 cut(s) 359, 386, 809
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 1 cut(s) 81
BalI TGGCCA 1 cut(s) 1103
BanI GGYRCC 1 cut(s) 70
BbsI GAAGAC 1 cut(s) 497
Bbv12I GWGCWC 1 cut(s) 487
BbvCI CCTCAGC 1 cut(s) 1161
BbvI GCAGC 3 cut(s) 198, 550, 553
BccI CCATC 1 cut(s) 1289
BceAI ACGGC 1 cut(s) 748
BciT130I CCWGG 1 cut(s) 320
BciVI GTATCC 1 cut(s) 837
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 5 cut(s) 720, 805, 1006, 1055, 1196
BfaI CTAG 6 cut(s) 356, 360, 387, 674, 810, 1329
BfmI CTRYAG 2 cut(s) 682, 1078
BfuI GTATCC 1 cut(s) 837
BglII AGATCT 1 cut(s) 1074
BisI GCNGC 6 cut(s) 74, 212, 564, 567, 700, 1227
BlsI GCNGC 6 cut(s) 75, 213, 565, 568, 701, 1228
Bme1390I CCNGG 1 cut(s) 320
Bme18I GGWCC 1 cut(s) 81
BmeRI GACNNNNNGTC 1 cut(s) 836
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 1 cut(s) 81
BmiI GGNNCC 2 cut(s) 72, 1129
BmrFI CCNGG 1 cut(s) 320
BmsI GCATC 3 cut(s) 138, 322, 972
BmtI GCTAGC 3 cut(s) 363, 390, 813
BpiI GAAGAC 1 cut(s) 497
Bpu10I CCTNAGC 3 cut(s) 26, 32, 1161
Bpu14I TTCGAA 1 cut(s) 190
BsaBI GATNNNNATC 1 cut(s) 336
BsaI GGTCTC 3 cut(s) 720, 1055, 1196
BsaJI CCNNGG 8 cut(s) 77, 370, 517, 730, 814, 934, 949, 1104
BsaWI WCCGGW 1 cut(s) 840
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse118I RCCGGY 1 cut(s) 1174
Bse3DI GCAATG 4 cut(s) 576, 759, 1020, 1104
Bse8I GATNNNNATC 1 cut(s) 336
BseAI TCCGGA 1 cut(s) 840
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 8 cut(s) 77, 370, 517, 730, 814, 934, 949, 1104
BseGI GGATG 1 cut(s) 337
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 4 cut(s) 576, 759, 1020, 1104
BseMII CTCAG 2 cut(s) 40, 1152
BsePI GCGCGC 1 cut(s) 314
BseRI GAGGAG 2 cut(s) 853, 938
BseXI GCAGC 3 cut(s) 198, 550, 553
BsgI GTGCAG 1 cut(s) 897
Bsh1236I CGCG 2 cut(s) 316, 936
Bsh1285I CGRYCG 1 cut(s) 1095
BshFI GGCC 4 cut(s) 702, 768, 924, 1103
BshNI GGYRCC 1 cut(s) 70
BsiEI CGRYCG 1 cut(s) 1095
BsiHKAI GWGCWC 1 cut(s) 487
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 2 cut(s) 841, 1175
BslFI GGGAC 1 cut(s) 1097
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 5 cut(s) 720, 805, 1006, 1055, 1196
BsmFI GGGAC 1 cut(s) 1097
BsnI GGCC 4 cut(s) 702, 768, 924, 1103
Bso31I GGTCTC 3 cut(s) 720, 1055, 1196
BsoBI CYCGRG 1 cut(s) 215
Bsp119I TTCGAA 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 487
Bsp13I TCCGGA 1 cut(s) 840
Bsp143I GATC 6 cut(s) 3, 818, 1074, 1092, 1230, 1325
Bsp19I CCATGG 2 cut(s) 77, 1104
BspACI CCGC 6 cut(s) 74, 699, 934, 936, 1178, 1226
BspANI GGCC 4 cut(s) 702, 768, 924, 1103
BspCNI CTCAG 2 cut(s) 39, 1153
BspEI TCCGGA 1 cut(s) 840
BspFNI CGCG 2 cut(s) 316, 936
BspHI TCATGA 1 cut(s) 1137
BspLI GGNNCC 2 cut(s) 72, 1129
BspOI GCTAGC 3 cut(s) 363, 390, 813
BspPI GGATC 3 cut(s) 826, 1225, 1333
BspT104I TTCGAA 1 cut(s) 190
BspT107I GGYRCC 1 cut(s) 70
BspTNI GGTCTC 3 cut(s) 720, 1055, 1196
BsrDI GCAATG 4 cut(s) 576, 759, 1020, 1104
BsrFI RCCGGY 1 cut(s) 1174
BssAI RCCGGY 1 cut(s) 1174
BssECI CCNNGG 8 cut(s) 77, 370, 517, 730, 814, 934, 949, 1104
BssHII GCGCGC 1 cut(s) 314
BssMI GATC 6 cut(s) 3, 818, 1074, 1092, 1230, 1325
BssT1I CCWWGG 4 cut(s) 77, 370, 517, 1104
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 1 cut(s) 482
Bst6I CTCTTC 1 cut(s) 863
BstBI TTCGAA 1 cut(s) 190
BstDEI CTNAG 4 cut(s) 26, 32, 125, 1161
BstDSI CCRYGG 4 cut(s) 77, 730, 934, 1104
BstF5I GGATG 1 cut(s) 337
BstFNI CGCG 2 cut(s) 316, 936
BstHHI GCGC 3 cut(s) 316, 318, 989
BstKTI GATC 6 cut(s) 6, 821, 1077, 1095, 1233, 1328
BstMAI GTCTC 5 cut(s) 720, 805, 1006, 1055, 1196
BstMBI GATC 6 cut(s) 3, 818, 1074, 1092, 1230, 1325
BstMCI CGRYCG 1 cut(s) 1095
BstMWI GCNNNNNNNGC 3 cut(s) 320, 770, 995
BstNI CCWGG 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 318
BstSFI CTRYAG 2 cut(s) 682, 1078
BstUI CGCG 2 cut(s) 316, 936
BstV1I GCAGC 3 cut(s) 198, 550, 553
BstV2I GAAGAC 1 cut(s) 497
BstX2I RGATCY 3 cut(s) 818, 1074, 1325
BstYI RGATCY 3 cut(s) 818, 1074, 1325
BsuI GTATCC 1 cut(s) 837
BsuRI GGCC 4 cut(s) 702, 768, 924, 1103
BtgI CCRYGG 4 cut(s) 77, 730, 934, 1104
BtgZI GCGATG 1 cut(s) 646
BtsCI GGATG 1 cut(s) 337
BtsI GCAGTG 2 cut(s) 982, 1340
BtsIMutI CAGTG 3 cut(s) 487, 982, 1340
CciI TCATGA 1 cut(s) 1137
CfoI GCGC 3 cut(s) 316, 318, 989
Cfr10I RCCGGY 1 cut(s) 1174
Cfr13I GGNCC 1 cut(s) 81
Cfr42I CCGCGG 1 cut(s) 937
CseI GACGC 1 cut(s) 1173
Csp6I GTAC 5 cut(s) 303, 478, 529, 931, 1069
CviQI GTAC 5 cut(s) 303, 478, 529, 931, 1069
DdeI CTNAG 4 cut(s) 26, 32, 125, 1161
DpnI GATC 6 cut(s) 5, 820, 1076, 1094, 1232, 1327
DpnII GATC 6 cut(s) 3, 818, 1074, 1092, 1230, 1325
DriI GACNNNNNGTC 1 cut(s) 836
EaeI YGGCCR 1 cut(s) 1101
Eam1104I CTCTTC 1 cut(s) 863
Eam1105I GACNNNNNGTC 1 cut(s) 836
EarI CTCTTC 1 cut(s) 863
Eco130I CCWWGG 4 cut(s) 77, 370, 517, 1104
Eco31I GGTCTC 3 cut(s) 720, 1055, 1196
Eco32I GATATC 1 cut(s) 429
Eco47I GGWCC 1 cut(s) 81
Eco88I CYCGRG 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 318
EcoRV GATATC 1 cut(s) 429
EcoT14I CCWWGG 4 cut(s) 77, 370, 517, 1104
EcoT22I ATGCAT 1 cut(s) 1268
ErhI CCWWGG 4 cut(s) 77, 370, 517, 1104
FaqI GGGAC 1 cut(s) 1097
FauNDI CATATG 1 cut(s) 179
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 6 cut(s) 74, 212, 564, 567, 700, 1227
FokI GGATG 1 cut(s) 344
Fsp4HI GCNGC 6 cut(s) 74, 212, 564, 567, 700, 1227
FspBI CTAG 6 cut(s) 356, 360, 387, 674, 810, 1329
GlaI GCGC 3 cut(s) 315, 317, 988
GluI GCNGC 6 cut(s) 74, 212, 564, 567, 700, 1227
HaeIII GGCC 4 cut(s) 702, 768, 924, 1103
HapII CCGG 2 cut(s) 841, 1175
HgaI GACGC 1 cut(s) 1173
HhaI GCGC 3 cut(s) 316, 318, 989
Hin6I GCGC 3 cut(s) 314, 316, 987
HinP1I GCGC 3 cut(s) 314, 316, 987
HindIII AAGCTT 2 cut(s) 115, 155
HinfI GANTC 8 cut(s) 37, 266, 347, 438, 513, 893, 1205, 1304
HpaII CCGG 2 cut(s) 841, 1175
HphI GGTGA 2 cut(s) 902, 1214
Hpy188I TCNGA 6 cut(s) 42, 552, 818, 953, 1092, 1288
Hpy188III TCNNGA 3 cut(s) 841, 1138, 1323
HpyAV CCTTC 1 cut(s) 688
HpyCH4III ACNGT 1 cut(s) 482
HpyCH4IV ACGT 1 cut(s) 642
HpyF10VI GCNNNNNNNGC 3 cut(s) 320, 770, 995
HpyF3I CTNAG 4 cut(s) 26, 32, 125, 1161
HpySE526I ACGT 1 cut(s) 642
HspAI GCGC 3 cut(s) 314, 316, 987
Kpn2I TCCGGA 1 cut(s) 840
Ksp22I TGATCA 1 cut(s) 3
KspI CCGCGG 1 cut(s) 937
Kzo9I GATC 6 cut(s) 3, 818, 1074, 1092, 1230, 1325
LmnI GCTCC 1 cut(s) 1133
Lsp1109I GCAGC 3 cut(s) 198, 550, 553
LweI GCATC 3 cut(s) 138, 322, 972
MaeI CTAG 6 cut(s) 356, 360, 387, 674, 810, 1329
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 3 cut(s) 521, 1036, 1055
MalI GATC 6 cut(s) 5, 820, 1076, 1094, 1232, 1327
MboI GATC 6 cut(s) 3, 818, 1074, 1092, 1230, 1325
MboII GAAGA 4 cut(s) 497, 745, 850, 1308
MfeI CAATTG 1 cut(s) 309
MflI RGATCY 3 cut(s) 818, 1074, 1325
MhlI GDGCHC 1 cut(s) 487
MlsI TGGCCA 1 cut(s) 1103
MluCI AATT 6 cut(s) 257, 309, 396, 546, 597, 744
MluNI TGGCCA 1 cut(s) 1103
MlyI GAGTC 3 cut(s) 31, 260, 1214
MnlI CCTC 9 cut(s) 61, 93, 713, 824, 874, 952, 959, 1156, 1286
Mox20I TGGCCA 1 cut(s) 1103
Mph1103I ATGCAT 1 cut(s) 1268
MroI TCCGGA 1 cut(s) 840
MscI TGGCCA 1 cut(s) 1103
MseI TTAA 4 cut(s) 56, 159, 260, 1029
MslI CAYNNNNRTG 1 cut(s) 1261
Msp20I TGGCCA 1 cut(s) 1103
MspA1I CMGCKG 3 cut(s) 422, 936, 1226
MspI CCGG 2 cut(s) 841, 1175
MspR9I CCNGG 1 cut(s) 320
MunI CAATTG 1 cut(s) 309
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 2 cut(s) 316, 936
MwoI GCNNNNNNNGC 3 cut(s) 320, 770, 995
NcoI CCATGG 2 cut(s) 77, 1104
NdeI CATATG 1 cut(s) 179
NdeII GATC 6 cut(s) 3, 818, 1074, 1092, 1230, 1325
NheI GCTAGC 3 cut(s) 359, 386, 809
NlaIV GGNNCC 2 cut(s) 72, 1129
NmeAIII GCCGAG 1 cut(s) 1104
NsiI ATGCAT 1 cut(s) 1268
NspV TTCGAA 1 cut(s) 190
PagI TCATGA 1 cut(s) 1137
PauI GCGCGC 1 cut(s) 314
PfeI GAWTC 5 cut(s) 347, 438, 513, 893, 1304
PflMI CCANNNNNTGG 1 cut(s) 401
PkrI GCNGC 6 cut(s) 75, 213, 565, 568, 701, 1228
Ple19I CGATCG 1 cut(s) 1095
PleI GAGTC 3 cut(s) 31, 260, 1213
PpsI GAGTC 3 cut(s) 31, 260, 1213
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 72, 1129
PspPI GGNCC 1 cut(s) 81
PsuI RGATCY 3 cut(s) 818, 1074, 1325
PteI GCGCGC 1 cut(s) 314
PvuI CGATCG 1 cut(s) 1095
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 5 cut(s) 304, 479, 530, 932, 1070
RsaNI GTAC 5 cut(s) 303, 478, 529, 931, 1069
RseI CAYNNNNRTG 1 cut(s) 1261
SacII CCGCGG 1 cut(s) 937
SaqAI TTAA 4 cut(s) 56, 159, 260, 1029
SatI GCNGC 6 cut(s) 74, 212, 564, 567, 700, 1227
Sau3AI GATC 6 cut(s) 3, 818, 1074, 1092, 1230, 1325
Sau96I GGNCC 1 cut(s) 81
SchI GAGTC 3 cut(s) 31, 260, 1214
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 1 cut(s) 487
SfaNI GCATC 3 cut(s) 138, 322, 972
SfcI CTRYAG 2 cut(s) 682, 1078
Sfr303I CCGCGG 1 cut(s) 937
SfuI TTCGAA 1 cut(s) 190
SgrAI CRCCGGYG 1 cut(s) 1174
SgrBI CCGCGG 1 cut(s) 937
SinI GGWCC 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 1261
Sse9I AATT 6 cut(s) 257, 309, 396, 546, 597, 744
SsiI CCGC 6 cut(s) 74, 699, 934, 936, 1178, 1226
SspMI CTAG 6 cut(s) 356, 360, 387, 674, 810, 1329
StyD4I CCNGG 1 cut(s) 318
StyI CCWWGG 4 cut(s) 77, 370, 517, 1104
TaaI ACNGT 1 cut(s) 482
TaiI ACGT 1 cut(s) 645
TaqI TCGA 2 cut(s) 190, 350
TasI AATT 6 cut(s) 257, 309, 396, 546, 597, 744
TatI WGTACW 1 cut(s) 1068
TauI GCSGC 3 cut(s) 76, 702, 1229
TfiI GAWTC 5 cut(s) 347, 438, 513, 893, 1304
Tru1I TTAA 4 cut(s) 56, 159, 260, 1029
Tru9I TTAA 4 cut(s) 56, 159, 260, 1029
TscAI CASTG 3 cut(s) 487, 989, 1340
TseI GCWGC 3 cut(s) 211, 563, 566
TspDTI ATGAA 7 cut(s) 497, 604, 745, 1126, 1283, 1296, 1308
TspRI CASTG 3 cut(s) 487, 989, 1340
Van91I CCANNNNNTGG 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 81
XapI RAATTY 1 cut(s) 546
XspI CTAG 6 cut(s) 356, 360, 387, 674, 810, 1329
Zsp2I ATGCAT 1 cut(s) 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.