Rroxscaffold_7G00173620

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001164
Physical Location & Seq
Reverse (-)
13582074 .. 13584009
1936 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00173620.1

Sequence Viewer

Length: 975 bp
ATGATCAAGCTAGAAAACATATGGCTCAAGCTAAGAAGGACTCTTATTAAAAAGGCATTAAGAGACAAGGAGGTGCCGCCATGGACCAAACAAATAACTATGAGATCTATGGTCGCAAGCTTTTTTCTTAGCATCATTTTTCACTTCCTCGTGTGCAAGCTTAATCTTACAACTAGTGTCATATGGTATATCGCGGCGGGGTTGTTGGGATTCACACTGCTGAAAGGATACACTGTCTTGCTTCAAAAGTTAGGTATGTTGAAGCAACCTTTCACTAGTCAAGAAAACACAGTGATTCAAATCTTTGTTATGGGTTCGTCTGCAATAGCCTTCAGCAGCGACATGACAAATTATTTACCATGGATGAGCCCTCTTGTAGCTGCTCAGGGAGATGCAGGCAACACTTTAATCAACATCAAGAGGCCTTCAATTCCATGGATGACGGTACATGATGAACGAAGAATTGATCGAGTACTTTTGAAAGACCAAATTCCGAGCTGGATTGCTGCTGCTGTCTACATTGCTCTTGCAATCATATCAATTATTGTCCTGCCATTCATGTTCCACCAACTAAAATGGTATCACGTTGCGATAGCTTACATCATCGCTCTGGTTCTAGCCTTCTGTAATGCCTATGATTGCGGCCTCACAGATTGGTCTCTTGCTTCCAACTACGGCAAAATCGCAATTCTCATCTTCAGTGCTTGGCTTGGCCTTGAAAAAGGTGGTGTCACTGCTGGCCTTGCTTCTTGTGGTGTAATGATGAGCATTGTCTCAACAGCCTCGGATCTCATGCAAGACTTCAAGACCGGATACCTAACTCTCTCTTCTCCTCGATCCATGTTCGTTAGCCAAGTTTTCGGGACAGCCATGGGGTGTGTCATGTCGCCTTTGATATTCTGGTTCTTCTACAAAGCTTACCGAGTTGGTGATCCAACAGGCTCATATCCTGCACGGCTGCACCCTATGGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

324

Amino Acids

36.26

Weight (kDa)

9.51

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 31 - 122 3.3e-10 OPT oligopeptide transporter protein
OPT PF03169 147 - 318 3.9e-47 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 73
AccI GTMKAC 1 cut(s) 516
AccII CGCG 1 cut(s) 194
AciI CCGC 4 cut(s) 77, 194, 197, 642
AclWI GGATC 3 cut(s) 795, 831, 926
AcsI RAATTY 1 cut(s) 489
AcuI CTGAAG 2 cut(s) 316, 682
AfaI GTAC 2 cut(s) 447, 474
AgsI TTSAA 7 cut(s) 245, 262, 299, 429, 481, 719, 805
AhlI ACTAGT 2 cut(s) 173, 275
AluBI AGCT 8 cut(s) 10, 31, 120, 160, 380, 498, 596, 917
AluI AGCT 8 cut(s) 10, 31, 120, 160, 380, 498, 596, 917
Alw26I GTCTC 3 cut(s) 57, 663, 778
AlwI GGATC 3 cut(s) 795, 831, 926
AoxI GGCC 4 cut(s) 422, 643, 712, 739
ApeKI GCWGC 5 cut(s) 336, 380, 506, 509, 958
ApoI RAATTY 1 cut(s) 489
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 941
AvaII GGWCC 1 cut(s) 84
BanI GGYRCC 1 cut(s) 73
BanII GRGCYC 1 cut(s) 371
BauI CACGAG 1 cut(s) 149
BbvI GCAGC 5 cut(s) 348, 367, 493, 496, 945
BceAI ACGGC 2 cut(s) 691, 971
BciVI GTATCC 2 cut(s) 221, 806
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 3 cut(s) 57, 663, 778
BcuI ACTAGT 2 cut(s) 173, 275
BfaI CTAG 4 cut(s) 11, 174, 276, 617
BfuI GTATCC 2 cut(s) 221, 806
BglII AGATCT 1 cut(s) 104
BisI GCNGC 8 cut(s) 77, 195, 337, 381, 507, 510, 643, 959
BlsI GCNGC 8 cut(s) 78, 196, 338, 382, 508, 511, 644, 960
BmcAI AGTACT 1 cut(s) 474
Bme18I GGWCC 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 1 cut(s) 75
BmsI GCATC 2 cut(s) 141, 382
Bpu10I CCTNAGC 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 11
BsaBI GATNNNNATC 1 cut(s) 299
BsaI GGTCTC 1 cut(s) 663
BsaJI CCNNGG 5 cut(s) 80, 359, 434, 783, 870
BsaWI WCCGGW 1 cut(s) 809
Bse3DI GCAATG 1 cut(s) 519
Bse8I GATNNNNATC 1 cut(s) 299
BseDI CCNNGG 5 cut(s) 80, 359, 434, 783, 870
BseGI GGATG 2 cut(s) 369, 444
BseJI GATNNNNATC 1 cut(s) 299
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 1 cut(s) 398
BseRI GAGGAG 1 cut(s) 822
BseXI GCAGC 5 cut(s) 348, 367, 493, 496, 945
BsgI GTGCAG 2 cut(s) 936, 944
Bsh1236I CGCG 1 cut(s) 194
BshFI GGCC 4 cut(s) 424, 645, 714, 741
BshNI GGYRCC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 810
BslFI GGGAC 1 cut(s) 877
BsmAI GTCTC 3 cut(s) 57, 663, 778
BsmFI GGGAC 1 cut(s) 877
BsnI GGCC 4 cut(s) 424, 645, 714, 741
Bso31I GGTCTC 1 cut(s) 663
Bsp1286I GDGCHC 1 cut(s) 371
Bsp143I GATC 6 cut(s) 3, 104, 466, 787, 836, 931
Bsp19I CCATGG 4 cut(s) 80, 359, 434, 870
BspACI CCGC 4 cut(s) 77, 194, 197, 642
BspANI GGCC 4 cut(s) 424, 645, 714, 741
BspCNI CTCAG 1 cut(s) 397
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 1 cut(s) 75
BspPI GGATC 3 cut(s) 795, 831, 926
BspT107I GGYRCC 1 cut(s) 73
BspTNI GGTCTC 1 cut(s) 663
BsrDI GCAATG 1 cut(s) 519
BssECI CCNNGG 5 cut(s) 80, 359, 434, 783, 870
BssMI GATC 6 cut(s) 3, 104, 466, 787, 836, 931
BssSI CACGAG 1 cut(s) 149
BssT1I CCWWGG 4 cut(s) 80, 359, 434, 870
Bst2BI CACGAG 1 cut(s) 149
Bst4CI ACNGT 3 cut(s) 235, 292, 445
Bst6I CTCTTC 1 cut(s) 832
BstC8I GCNNGC 4 cut(s) 118, 158, 397, 739
BstDEI CTNAG 3 cut(s) 32, 128, 384
BstDSI CCRYGG 4 cut(s) 80, 359, 434, 870
BstF5I GGATG 2 cut(s) 369, 444
BstFNI CGCG 1 cut(s) 194
BstKTI GATC 6 cut(s) 6, 107, 469, 790, 839, 934
BstMAI GTCTC 3 cut(s) 57, 663, 778
BstMBI GATC 6 cut(s) 3, 104, 466, 787, 836, 931
BstMWI GCNNNNNNNGC 1 cut(s) 743
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 5 cut(s) 348, 367, 493, 496, 945
BstX2I RGATCY 2 cut(s) 104, 787
BstYI RGATCY 2 cut(s) 104, 787
BsuI GTATCC 2 cut(s) 221, 806
BsuRI GGCC 4 cut(s) 424, 645, 714, 741
BtgI CCRYGG 4 cut(s) 80, 359, 434, 870
BtgZI GCGATG 1 cut(s) 589
BtsCI GGATG 2 cut(s) 369, 444
BtsI GCAGTG 2 cut(s) 215, 732
BtsIMutI CAGTG 5 cut(s) 215, 231, 297, 706, 732
Cac8I GCNNGC 4 cut(s) 118, 158, 397, 739
Cfr13I GGNCC 1 cut(s) 84
Csp6I GTAC 2 cut(s) 446, 473
CviQI GTAC 2 cut(s) 446, 473
DdeI CTNAG 3 cut(s) 32, 128, 384
DpnI GATC 6 cut(s) 5, 106, 468, 789, 838, 933
DpnII GATC 6 cut(s) 3, 104, 466, 787, 836, 931
Eam1104I CTCTTC 1 cut(s) 832
EarI CTCTTC 1 cut(s) 832
Eco130I CCWWGG 4 cut(s) 80, 359, 434, 870
Eco147I AGGCCT 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 371
Eco31I GGTCTC 1 cut(s) 663
Eco47I GGWCC 1 cut(s) 84
Eco57I CTGAAG 2 cut(s) 316, 682
EcoT14I CCWWGG 4 cut(s) 80, 359, 434, 870
EcoT38I GRGCYC 1 cut(s) 371
ErhI CCWWGG 4 cut(s) 80, 359, 434, 870
FaqI GGGAC 1 cut(s) 877
FauI CCCGC 1 cut(s) 190
FauNDI CATATG 2 cut(s) 20, 182
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 8 cut(s) 77, 195, 337, 381, 507, 510, 643, 959
FokI GGATG 2 cut(s) 376, 451
FriOI GRGCYC 1 cut(s) 371
Fsp4HI GCNGC 8 cut(s) 77, 195, 337, 381, 507, 510, 643, 959
FspBI CTAG 4 cut(s) 11, 174, 276, 617
GluI GCNGC 8 cut(s) 77, 195, 337, 381, 507, 510, 643, 959
HaeIII GGCC 4 cut(s) 424, 645, 714, 741
HapII CCGG 1 cut(s) 810
HindIII AAGCTT 3 cut(s) 118, 158, 915
HinfI GANTC 3 cut(s) 40, 210, 295
HpaII CCGG 1 cut(s) 810
HphI GGTGA 1 cut(s) 941
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 2 cut(s) 495, 787
Hpy188III TCNNGA 4 cut(s) 281, 418, 805, 862
Hpy8I GTNNAC 1 cut(s) 517
HpyAV CCTTC 4 cut(s) 30, 340, 435, 631
HpyCH4III ACNGT 3 cut(s) 235, 292, 445
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 7 cut(s) 156, 323, 395, 530, 796, 953, 961
HpyF10VI GCNNNNNNNGC 1 cut(s) 743
HpyF3I CTNAG 3 cut(s) 32, 128, 384
HpySE526I ACGT 1 cut(s) 585
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 6 cut(s) 3, 104, 466, 787, 836, 931
Lsp1109I GCAGC 5 cut(s) 348, 367, 493, 496, 945
LweI GCATC 2 cut(s) 141, 382
MaeI CTAG 4 cut(s) 11, 174, 276, 617
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 1 cut(s) 730
MalI GATC 6 cut(s) 5, 106, 468, 789, 838, 933
MboI GATC 6 cut(s) 3, 104, 466, 787, 836, 931
MboII GAAGA 4 cut(s) 471, 688, 819, 898
MflI RGATCY 2 cut(s) 104, 787
MhlI GDGCHC 1 cut(s) 371
MluCI AATT 6 cut(s) 349, 429, 462, 489, 540, 687
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 2 cut(s) 693, 959
MnlI CCTC 7 cut(s) 64, 158, 381, 414, 656, 793, 843
MseI TTAA 4 cut(s) 48, 59, 162, 407
MspI CCGG 1 cut(s) 810
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 1 cut(s) 743
NcoI CCATGG 4 cut(s) 80, 359, 434, 870
NdeI CATATG 2 cut(s) 20, 182
NdeII GATC 6 cut(s) 3, 104, 466, 787, 836, 931
NlaIV GGNNCC 1 cut(s) 75
NmuCI GTSAC 1 cut(s) 730
PceI AGGCCT 1 cut(s) 424
PfeI GAWTC 2 cut(s) 210, 295
PkrI GCNGC 8 cut(s) 78, 196, 338, 382, 508, 511, 644, 960
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
PspN4I GGNNCC 1 cut(s) 75
PspPI GGNCC 1 cut(s) 84
PsuI RGATCY 2 cut(s) 104, 787
RsaI GTAC 2 cut(s) 447, 474
RsaNI GTAC 2 cut(s) 446, 473
SaqAI TTAA 4 cut(s) 48, 59, 162, 407
SatI GCNGC 8 cut(s) 77, 195, 337, 381, 507, 510, 643, 959
Sau3AI GATC 6 cut(s) 3, 104, 466, 787, 836, 931
Sau96I GGNCC 1 cut(s) 84
ScaI AGTACT 1 cut(s) 474
SchI GAGTC 1 cut(s) 34
SduI GDGCHC 1 cut(s) 371
SfaNI GCATC 2 cut(s) 141, 382
SinI GGWCC 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
SpeI ACTAGT 2 cut(s) 173, 275
Sse9I AATT 6 cut(s) 349, 429, 462, 489, 540, 687
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 4 cut(s) 77, 194, 197, 642
SspMI CTAG 4 cut(s) 11, 174, 276, 617
StuI AGGCCT 1 cut(s) 424
StyI CCWWGG 4 cut(s) 80, 359, 434, 870
TaaI ACNGT 3 cut(s) 235, 292, 445
TaiI ACGT 1 cut(s) 588
TaqI TCGA 2 cut(s) 469, 835
TasI AATT 6 cut(s) 349, 429, 462, 489, 540, 687
TatI WGTACW 1 cut(s) 472
TauI GCSGC 3 cut(s) 79, 197, 645
TfiI GAWTC 2 cut(s) 210, 295
Tru1I TTAA 4 cut(s) 48, 59, 162, 407
Tru9I TTAA 4 cut(s) 48, 59, 162, 407
TscAI CASTG 5 cut(s) 222, 238, 297, 706, 739
TseFI GTSAC 1 cut(s) 730
TseI GCWGC 5 cut(s) 336, 380, 506, 509, 958
Tsp45I GTSAC 1 cut(s) 730
TspDTI ATGAA 2 cut(s) 468, 547
TspRI CASTG 5 cut(s) 222, 238, 297, 706, 739
VpaK11BI GGWCC 1 cut(s) 84
XapI RAATTY 1 cut(s) 489
XmiI GTMKAC 1 cut(s) 516
XspI CTAG 4 cut(s) 11, 174, 276, 617
ZrmI AGTACT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.