Rmu_co8173216.1_g000001

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8173216.1
OG0001164
Physical Location & Seq
Forward (+)
1 .. 676
676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8173216.1_g000001.1.cds

Sequence Viewer

Length: 676 bp
cagaagaaaagaaagtagagttggcatcgcctttgccgccaccactatcattatccaatatggaagctggcggattgccctcatcagaaactgagctgagtgaaaactacgacgaggaacgaagaactgaatacttcttgaaagaccaaattccactctcggttgctgtcgctggctacattgctcttgcaggcatatcaattattgtcctgccattcattttccaccaactaaaatggtatcatgttctgacagcttacttaatcgctccggttctggccttctgcaatgcctatggctgcggcctcacagattggtcccttgcttccaactacggcaaaatcgcaatcctgatcttcagtgcttgggttggccttgaaaaaggtggtgtcattgctggcctcgcttcttgtggtgcaatgatgagcattgtctcgacagcctccgatctcatgcaagacttcaagactggatacctaactctctcgtctcctcgatccatgttcttcagccaagttttcgggacagccatggggtgtgtcatgtcgcctttgatattctggttcttctacaaagcttaccgagttggtgatcccacgggatcatatcctgcaccctatggcctaatgtaccgtggcattgcactcctcggagttgagggctttggctcactccc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

225

Amino Acids

24.41

Weight (kDa)

5.01

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 37, 71, 302
AclWI GGATC 3 cut(s) 491, 586, 609
AcsI RAATTY 1 cut(s) 149
AcuI CTGAAG 2 cut(s) 342, 492
AfaI GTAC 1 cut(s) 631
AgsI TTSAA 3 cut(s) 141, 379, 465
AjuI GAANNNNNNNTTGG 1 cut(s) 36
AluBI AGCT 4 cut(s) 67, 96, 256, 577
AluI AGCT 4 cut(s) 67, 96, 256, 577
Alw26I GTCTC 2 cut(s) 438, 494
AlwI GGATC 3 cut(s) 491, 586, 609
AlwNI CAGNNNCTG 1 cut(s) 91
AoxI GGCC 5 cut(s) 278, 303, 372, 399, 621
ApeKI GCWGC 1 cut(s) 299
ApoI RAATTY 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 317
AsuHPI GGTGA 1 cut(s) 601
AvaII GGWCC 1 cut(s) 317
BbvI GCAGC 1 cut(s) 286
BceAI ACGGC 1 cut(s) 351
BciVI GTATCC 1 cut(s) 466
BcoDI GTCTC 2 cut(s) 438, 494
BfuI GTATCC 1 cut(s) 466
BisI GCNGC 3 cut(s) 37, 300, 303
BlsI GCNGC 3 cut(s) 38, 301, 304
Bme18I GGWCC 1 cut(s) 317
BmgT120I GGNCC 1 cut(s) 317
BmiI GGNNCC 1 cut(s) 319
BmsI GCATC 1 cut(s) 34
BsaJI CCNNGG 4 cut(s) 530, 596, 633, 648
BsaWI WCCGGW 1 cut(s) 270
Bse1I ACTGG 1 cut(s) 474
Bse3DI GCAATG 5 cut(s) 179, 294, 392, 425, 638
BseDI CCNNGG 4 cut(s) 530, 596, 633, 648
BseMI GCAATG 5 cut(s) 179, 294, 392, 425, 638
BseMII CTCAG 2 cut(s) 83, 88
BseNI ACTGG 1 cut(s) 474
BseRI GAGGAG 2 cut(s) 482, 637
BseXI GCAGC 1 cut(s) 286
BsgI GTGCAG 1 cut(s) 596
BshFI GGCC 5 cut(s) 280, 305, 374, 401, 623
BsiSI CCGG 1 cut(s) 271
BslFI GGGAC 2 cut(s) 303, 537
BsmAI GTCTC 2 cut(s) 438, 494
BsmBI CGTCTC 1 cut(s) 494
BsmFI GGGAC 2 cut(s) 303, 537
BsnI GGCC 5 cut(s) 280, 305, 374, 401, 623
Bsp143I GATC 5 cut(s) 353, 447, 496, 591, 601
Bsp19I CCATGG 1 cut(s) 530
BspACI CCGC 3 cut(s) 37, 71, 302
BspANI GGCC 5 cut(s) 280, 305, 374, 401, 623
BspCNI CTCAG 2 cut(s) 84, 89
BspLI GGNNCC 1 cut(s) 319
BspPI GGATC 3 cut(s) 491, 586, 609
BsrDI GCAATG 5 cut(s) 179, 294, 392, 425, 638
BsrI ACTGG 1 cut(s) 474
BssECI CCNNGG 4 cut(s) 530, 596, 633, 648
BssMI GATC 5 cut(s) 353, 447, 496, 591, 601
BssT1I CCWWGG 1 cut(s) 530
Bst4CI ACNGT 1 cut(s) 634
BstC8I GCNNGC 4 cut(s) 69, 174, 192, 399
BstDEI CTNAG 2 cut(s) 92, 97
BstDSI CCRYGG 3 cut(s) 530, 596, 633
BstKTI GATC 5 cut(s) 356, 450, 499, 594, 604
BstMAI GTCTC 2 cut(s) 438, 494
BstMBI GATC 5 cut(s) 353, 447, 496, 591, 601
BstMWI GCNNNNNNNGC 2 cut(s) 36, 403
BstV1I GCAGC 1 cut(s) 286
BsuI GTATCC 1 cut(s) 466
BsuRI GGCC 5 cut(s) 280, 305, 374, 401, 623
BtgI CCRYGG 3 cut(s) 530, 596, 633
BtgZI GCGATG 1 cut(s) 11
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 4 cut(s) 69, 174, 192, 399
CaiI CAGNNNCTG 1 cut(s) 91
Cfr13I GGNCC 1 cut(s) 317
Csp6I GTAC 1 cut(s) 630
CviAII CATG 5 cut(s) 244, 453, 501, 531, 543
CviQI GTAC 1 cut(s) 630
DdeI CTNAG 2 cut(s) 92, 97
DpnI GATC 5 cut(s) 355, 449, 498, 593, 603
DpnII GATC 5 cut(s) 353, 447, 496, 591, 601
EciI GGCGGA 1 cut(s) 86
Eco130I CCWWGG 1 cut(s) 530
Eco47I GGWCC 1 cut(s) 317
Eco57I CTGAAG 2 cut(s) 342, 492
EcoT14I CCWWGG 1 cut(s) 530
ErhI CCWWGG 1 cut(s) 530
Esp3I CGTCTC 1 cut(s) 494
FaeI CATG 5 cut(s) 247, 456, 504, 534, 546
FaqI GGGAC 2 cut(s) 303, 537
FatI CATG 5 cut(s) 243, 452, 500, 530, 542
Fnu4HI GCNGC 3 cut(s) 37, 300, 303
Fsp4HI GCNGC 3 cut(s) 37, 300, 303
GluI GCNGC 3 cut(s) 37, 300, 303
HaeIII GGCC 5 cut(s) 280, 305, 374, 401, 623
HapII CCGG 1 cut(s) 271
Hin1II CATG 5 cut(s) 247, 456, 504, 534, 546
HindIII AAGCTT 1 cut(s) 575
HpaII CCGG 1 cut(s) 271
HphI GGTGA 1 cut(s) 601
Hpy188I TCNGA 4 cut(s) 87, 251, 447, 652
Hpy188III TCNNGA 5 cut(s) 138, 351, 435, 465, 522
Hpy99I CGWCG 1 cut(s) 115
HpyAV CCTTC 1 cut(s) 291
HpyCH4III ACNGT 1 cut(s) 634
HpyCH4V TGCA 6 cut(s) 190, 287, 418, 456, 613, 643
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 403
HpyF3I CTNAG 2 cut(s) 92, 97
Hsp92II CATG 5 cut(s) 247, 456, 504, 534, 546
Kzo9I GATC 5 cut(s) 353, 447, 496, 591, 601
LmnI GCTCC 1 cut(s) 273
Lsp1109I GCAGC 1 cut(s) 286
LweI GCATC 1 cut(s) 34
MalI GATC 5 cut(s) 355, 449, 498, 593, 603
MboI GATC 5 cut(s) 353, 447, 496, 591, 601
MboII GAAGA 5 cut(s) 16, 134, 348, 498, 558
MluCI AATT 2 cut(s) 149, 200
MmeI TCCRAC 1 cut(s) 353
MnlI CCTC 8 cut(s) 90, 108, 316, 412, 453, 503, 651, 658
MseI TTAA 1 cut(s) 262
MspI CCGG 1 cut(s) 271
MwoI GCNNNNNNNGC 2 cut(s) 36, 403
NcoI CCATGG 1 cut(s) 530
NdeII GATC 5 cut(s) 353, 447, 496, 591, 601
NlaIII CATG 5 cut(s) 247, 456, 504, 534, 546
NlaIV GGNNCC 1 cut(s) 319
PkrI GCNGC 3 cut(s) 38, 301, 304
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 1 cut(s) 317
PstNI CAGNNNCTG 1 cut(s) 91
RsaI GTAC 1 cut(s) 631
RsaNI GTAC 1 cut(s) 630
SaqAI TTAA 1 cut(s) 262
SatI GCNGC 3 cut(s) 37, 300, 303
Sau3AI GATC 5 cut(s) 353, 447, 496, 591, 601
Sau96I GGNCC 1 cut(s) 317
SetI ASST 6 cut(s) 69, 98, 258, 387, 479, 579
SfaNI GCATC 1 cut(s) 34
SinI GGWCC 1 cut(s) 317
Sse9I AATT 2 cut(s) 149, 200
SsiI CCGC 3 cut(s) 37, 71, 302
StyI CCWWGG 1 cut(s) 530
TaaI ACNGT 1 cut(s) 634
TaqI TCGA 2 cut(s) 436, 495
TasI AATT 2 cut(s) 149, 200
TauI GCSGC 2 cut(s) 39, 305
Tru1I TTAA 1 cut(s) 262
Tru9I TTAA 1 cut(s) 262
TscAI CASTG 1 cut(s) 366
TseI GCWGC 1 cut(s) 299
TspDTI ATGAA 1 cut(s) 207
TspRI CASTG 1 cut(s) 366
VpaK11BI GGWCC 1 cut(s) 317
XapI RAATTY 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.