Rh2DG328200

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001164
Physical Location & Seq
Forward (+)
41507229 .. 41509698
2470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG328200.1

Sequence Viewer

Length: 2175 bp
ATGGAGACTAACGATTTCGACAATGTTCAAGCTGGAAAACAAATGGTTCAGGCTAGAGACTCGACTGAAAAGGCATTCAGAGACACAGAGGTGCCCCCATGGACCATGCAAATAACGGTCAGGTCTATGGTCACAAGCTTTTTTCTTAGCATCATCTTCAACTTCATCGTGTGCAAGCTCAATCTTACCACCGGTGTAATACCATCTCTAAACGTCGCAGCGGGGCTGTTGGGATTCGCACTGCTGAAAGGATACACTGCCTTGCTTCAAAAGTTAGGTCTGTTGAAGCAGCCTTTCACTCGTCAAGAAAACACAGTGGTTCAAACCTGTGTTGTGGCGTCGTCTGGGATAGCCTTCAGCAGTGGCACGGCGAGTTATTTACTAGGTATGAGCCCTCAGGTAGCTGCTCAGGGAGATGCTGGAAACACTCTAATCAACATTAAGAGGCTTTCGATTCCCTGGATGACGGGATATCTCTTCACAGTCAGCTTCGCTGGGCTTTTTTCGATAATAGCCTTGAGAGAGATGATGATCCTCAAAAGAAAACTGACCTACCCAAGTGGAACAGCAACAGCGCACCTCATCAACAGCTTCCACACCCCCAAAGGAGCTAGGATGGCAGCGAGACAAGTTTCTGCTCTCTTCAAGAGCTGCTGCGGCAGCTTTGTCTGGTCTTTTTTCCAGTGGTTTTTCGCAGCTTCGGATGGCTGTGGGTTTTCTAGCTTTCCAATTTTTGGTCTAAAAGCGTATAAACAAAGGTTTTACTTCGACTTCTCAACCACATATATTGGTTGTGGAATGATCACCTCTTACATGGTGAACATATCCATGCTCGTTGGGGCTGTGATATCATGGGGAATCATGTGGCCTTTGATTGAACAAAAGAAAGGTATCTGGTATGGAGCTCATCTTTCTGCAAGTAGTCTTCATGGAATCCAAGGTTACAGGGTCTTTATCTCCATAGGCATGATACTTGGTGATGGACTATACCATGTTATGTACCATGCTGGTTATATGGGTTTCATTCTCATTAAAAATCTCTATCTCATTAAAAATCTCTATAGCAACATTCTCATTAAAAATCTCTTTAGCAATAAACCAGAAGAAAAGAAAGTAGAGTTGGCATCACCTTCGCCGCCACCACTATCATTATCCAATATGGAAGCTGGCGGATTGCCCTCATCAGAAACTGAGCTGAGTGAAAACTACGACGAGGAACGAAGAACTGAGTACTTCTTGAAAGACCAAATTCCACTCTCGGTTGCTGTCGCTGGCTACATTGCTCTTGCAGGCATATCAATTATTGTCCTGCCATTCATTTTCCACCAACTAAAATGGTATCATGTTCTGACAGCTTACTTAATCGCTCCGGTTCTGGCCTTCTGCAATGCCTATGGCTGCGGCCTCACAGATTGGTCCCTTGCTTCCAACTACGGCAAAATCGCAATCCTGATCTTCAGTGCTTGGGTTGGCCTTGAAAAAGGTGGTGTCATTGCTGGCCTCGCTTCTTGTGGTGCAATGATGAGCATTGTCTCGACAGCCTCCGATCTCATGCAAGACTTCAAGACTGGATACCTAACTCTCTCGTCTCCTCGATCCATGTTCTTCAGCCAAGTTTTCGGGACAGCCATGGGGTGTGTCATGTCGCCTTTGATATTCTGGTTCTTCTACAAAGCTTACCGAGTTGGTGATCCCACGGGATCATATCCTGCACCCTATGGCCTAATGTACCGTGGCATTGCACTCCTCGGAGTTGAGGGCTTTGGCTCACTCCCTAAACACTGCGTCACACTTGCAGTCTCCTTTTTTGCATTTGCCATTGCCATTAACATAGTAACCGAGCTTTTGAAGCGTTACGAGACCAAGTACAAGATCTACAGGTTTGTTCCGAATGCAATGGCCATGGCAGTCCCATTCTATCTCGGCTCCTACTTCGTGATTGACATGTGTTTGGGAAGCTTGGTCCTTTTCTGCTGGAAGTTATGGAACAAAAAACAGGCGGAGGATTTGGCACCGGCGGTTGCATCTGGTCTCATCTGTGGCGAGTCTATGTGGGGTGTTCCGGCTGCGATCCTATCTCTGGCCAATGTCAAAGCACCAATATGCATGAAGTTCCTCTCTTACTCGGACAACGATAGAGTTGATTCGTTTTTATCTTCATCAGGATCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

724

Amino Acids

79.61

Weight (kDa)

8.96

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 37 - 692 1.3e-147 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 91, 2011
AccB7I CCANNNNNTGG 1 cut(s) 734
AciI CCGC 7 cut(s) 221, 657, 1136, 1170, 1401, 2000, 2018
AclWI GGATC 6 cut(s) 526, 1590, 1685, 1708, 2065, 2173
AcoI YGGCCR 2 cut(s) 1899, 2082
AcsI RAATTY 1 cut(s) 1248
AcuI CTGAAG 3 cut(s) 340, 1441, 1591
AcyI GRCGYC 1 cut(s) 338
AfaI GTAC 4 cut(s) 1001, 1232, 1730, 1868
AfiI CCNNNNNNNGG 2 cut(s) 734, 2080
AflIII ACRYGT 1 cut(s) 1944
AgeI ACCGGT 1 cut(s) 191
AjnI CCWGG 1 cut(s) 458
AjuI GAANNNNNNNTTGG 2 cut(s) 1103, 1135
AleI CACNNNNGTG 1 cut(s) 89
Alw21I GWGCWC 1 cut(s) 907
Alw26I GTCTC 8 cut(s) 51, 75, 619, 1537, 1593, 1804, 1853, 2036
AlwI GGATC 6 cut(s) 526, 1590, 1685, 1708, 2065, 2173
AlwNI CAGNNNCTG 1 cut(s) 1190
AoxI GGCC 8 cut(s) 866, 1377, 1402, 1471, 1498, 1720, 1899, 2082
ApoI RAATTY 1 cut(s) 1248
ArsI GACNNNNNNTTYG 2 cut(s) 262, 294
AsiGI ACCGGT 1 cut(s) 191
AspLEI GCGC 1 cut(s) 577
AspS9I GGNCC 3 cut(s) 102, 1416, 1963
AsuHPI GGTGA 5 cut(s) 796, 829, 989, 1119, 1700
AvaII GGWCC 3 cut(s) 102, 1416, 1963
AxyI CCTNAGG 1 cut(s) 396
BaeGI GKGCMC 1 cut(s) 96
BalI TGGCCA 2 cut(s) 1901, 2084
BanI GGYRCC 2 cut(s) 91, 2011
BanII GRGCYC 2 cut(s) 395, 907
BbsI GAAGAC 1 cut(s) 917
Bbv12I GWGCWC 1 cut(s) 907
BccI CCATC 4 cut(s) 211, 610, 698, 974
BceAI ACGGC 2 cut(s) 384, 1450
BcgI CGANNNNNNTGC 2 cut(s) 1113, 1147
BciT130I CCWGG 1 cut(s) 460
BciVI GTATCC 2 cut(s) 245, 1565
BclI TGATCA 1 cut(s) 801
BcoDI GTCTC 8 cut(s) 51, 75, 619, 1537, 1593, 1804, 1853, 2036
BfaI CTAG 6 cut(s) 54, 383, 612, 720, 2169, 2173
BfmI CTRYAG 2 cut(s) 1060, 1876
BfuI GTATCC 2 cut(s) 245, 1565
BglII AGATCT 1 cut(s) 1872
BmcAI AGTACT 1 cut(s) 1232
Bme1390I CCNGG 1 cut(s) 460
Bme18I GGWCC 3 cut(s) 102, 1416, 1963
BmgT120I GGNCC 3 cut(s) 102, 1416, 1963
BmiI GGNNCC 4 cut(s) 93, 1418, 1927, 2013
BmrFI CCNGG 1 cut(s) 460
BmsI GCATC 4 cut(s) 159, 406, 1133, 2033
BpiI GAAGAC 1 cut(s) 917
Bpu10I CCTNAGC 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 538
BsaBI GATNNNNATC 1 cut(s) 530
BsaHI GRCGYC 1 cut(s) 338
BsaI GGTCTC 2 cut(s) 1853, 2036
BsaJI CCNNGG 8 cut(s) 98, 458, 937, 1629, 1695, 1732, 1747, 1902
BsaWI WCCGGW 2 cut(s) 191, 1369
Bsc4I CCNNNNNNNGG 2 cut(s) 734, 2080
Bse118I RCCGGY 2 cut(s) 191, 2014
Bse1I ACTGG 2 cut(s) 682, 1573
Bse21I CCTNAGG 1 cut(s) 396
Bse3DI GCAATG 7 cut(s) 1278, 1393, 1491, 1524, 1737, 1818, 1902
Bse8I GATNNNNATC 1 cut(s) 530
BseBI CCWGG 1 cut(s) 460
BseDI CCNNGG 8 cut(s) 98, 458, 937, 1629, 1695, 1732, 1747, 1902
BseGI GGATG 3 cut(s) 468, 621, 709
BseJI GATNNNNATC 1 cut(s) 530
BseLI CCNNNNNNNGG 2 cut(s) 734, 2080
BseMI GCAATG 7 cut(s) 1278, 1393, 1491, 1524, 1737, 1818, 1902
BseMII CTCAG 5 cut(s) 410, 422, 1182, 1187, 1218
BseNI ACTGG 2 cut(s) 682, 1573
BseRI GAGGAG 2 cut(s) 1581, 1736
BseSI GKGCMC 1 cut(s) 96
BseYI CCCAGC 1 cut(s) 494
BsgI GTGCAG 1 cut(s) 1695
BshFI GGCC 8 cut(s) 868, 1379, 1404, 1473, 1500, 1722, 1901, 2084
BshNI GGYRCC 2 cut(s) 91, 2011
BshTI ACCGGT 1 cut(s) 191
BsiHKAI GWGCWC 1 cut(s) 907
BsiSI CCGG 4 cut(s) 192, 1370, 2015, 2063
BslFI GGGAC 3 cut(s) 1402, 1636, 1895
BslI CCNNNNNNNGG 2 cut(s) 734, 2080
BsmAI GTCTC 8 cut(s) 51, 75, 619, 1537, 1593, 1804, 1853, 2036
BsmBI CGTCTC 1 cut(s) 1593
BsmFI GGGAC 3 cut(s) 1402, 1636, 1895
BsmI GAATGC 2 cut(s) 74, 1897
BsnI GGCC 8 cut(s) 868, 1379, 1404, 1473, 1500, 1722, 1901, 2084
Bso31I GGTCTC 2 cut(s) 1853, 2036
Bsp1286I GDGCHC 3 cut(s) 96, 395, 907
Bsp19I CCATGG 3 cut(s) 98, 1629, 1902
BspACI CCGC 7 cut(s) 221, 657, 1136, 1170, 1401, 2000, 2018
BspANI GGCC 8 cut(s) 868, 1379, 1404, 1473, 1500, 1722, 1901, 2084
BspCNI CTCAG 5 cut(s) 409, 421, 1183, 1188, 1219
BspLI GGNNCC 4 cut(s) 93, 1418, 1927, 2013
BspPI GGATC 6 cut(s) 526, 1590, 1685, 1708, 2065, 2173
BspT107I GGYRCC 2 cut(s) 91, 2011
BspTNI GGTCTC 2 cut(s) 1853, 2036
BsrDI GCAATG 7 cut(s) 1278, 1393, 1491, 1524, 1737, 1818, 1902
BsrFI RCCGGY 2 cut(s) 191, 2014
BsrI ACTGG 2 cut(s) 682, 1573
BssAI RCCGGY 2 cut(s) 191, 2014
BssECI CCNNGG 8 cut(s) 98, 458, 937, 1629, 1695, 1732, 1747, 1902
BssNI GRCGYC 1 cut(s) 338
BssT1I CCWWGG 4 cut(s) 98, 937, 1629, 1902
Bst2UI CCWGG 1 cut(s) 460
Bst4CI ACNGT 4 cut(s) 118, 316, 484, 1733
Bst6I CTCTTC 2 cut(s) 482, 647
BstACI GRCGYC 1 cut(s) 338
BstC8I GCNNGC 5 cut(s) 176, 1168, 1273, 1291, 1498
BstDEI CTNAG 6 cut(s) 146, 396, 408, 1191, 1196, 1227
BstDSI CCRYGG 5 cut(s) 98, 1629, 1695, 1732, 1902
BstF5I GGATG 3 cut(s) 468, 621, 709
BstHHI GCGC 1 cut(s) 577
BstMAI GTCTC 8 cut(s) 51, 75, 619, 1537, 1593, 1804, 1853, 2036
BstMWI GCNNNNNNNGC 5 cut(s) 617, 657, 660, 1502, 1849
BstNI CCWGG 1 cut(s) 460
BstNSI RCATGY 1 cut(s) 1948
BstSCI CCNGG 1 cut(s) 458
BstSFI CTRYAG 2 cut(s) 1060, 1876
BstSLI GKGCMC 1 cut(s) 96
BstV2I GAAGAC 1 cut(s) 917
BstX2I RGATCY 2 cut(s) 1872, 2165
BstYI RGATCY 2 cut(s) 1872, 2165
Bsu36I CCTNAGG 1 cut(s) 396
BsuI GTATCC 2 cut(s) 245, 1565
BsuRI GGCC 8 cut(s) 868, 1379, 1404, 1473, 1500, 1722, 1901, 2084
BtgI CCRYGG 5 cut(s) 98, 1629, 1695, 1732, 1902
BtsCI GGATG 3 cut(s) 468, 621, 709
BtsI GCAGTG 4 cut(s) 239, 255, 367, 1780
BtsIMutI CAGTG 7 cut(s) 239, 255, 321, 367, 689, 1465, 1780
Cac8I GCNNGC 5 cut(s) 176, 1168, 1273, 1291, 1498
CaiI CAGNNNCTG 1 cut(s) 1190
CfoI GCGC 1 cut(s) 577
Cfr10I RCCGGY 2 cut(s) 191, 2014
Cfr13I GGNCC 3 cut(s) 102, 1416, 1963
CseI GACGC 2 cut(s) 327, 1774
Csp6I GTAC 4 cut(s) 1000, 1231, 1729, 1867
CspAI ACCGGT 1 cut(s) 191
CspCI CAANNNNNGTGG 2 cut(s) 769, 804
CviQI GTAC 4 cut(s) 1000, 1231, 1729, 1867
DdeI CTNAG 6 cut(s) 146, 396, 408, 1191, 1196, 1227
EaeI YGGCCR 2 cut(s) 1899, 2082
Eam1104I CTCTTC 2 cut(s) 482, 647
EarI CTCTTC 2 cut(s) 482, 647
EciI GGCGGA 2 cut(s) 1185, 2015
Ecl136II GAGCTC 1 cut(s) 905
Eco130I CCWWGG 4 cut(s) 98, 937, 1629, 1902
Eco24I GRGCYC 2 cut(s) 395, 907
Eco31I GGTCTC 2 cut(s) 1853, 2036
Eco32I GATATC 2 cut(s) 473, 849
Eco47I GGWCC 3 cut(s) 102, 1416, 1963
Eco53kI GAGCTC 1 cut(s) 905
Eco57I CTGAAG 3 cut(s) 340, 1441, 1591
Eco81I CCTNAGG 1 cut(s) 396
EcoICRI GAGCTC 1 cut(s) 905
EcoRII CCWGG 1 cut(s) 458
EcoRV GATATC 2 cut(s) 473, 849
EcoT14I CCWWGG 4 cut(s) 98, 937, 1629, 1902
EcoT22I ATGCAT 1 cut(s) 2108
EcoT38I GRGCYC 2 cut(s) 395, 907
ErhI CCWWGG 4 cut(s) 98, 937, 1629, 1902
Esp3I CGTCTC 1 cut(s) 1593
FaqI GGGAC 3 cut(s) 1402, 1636, 1895
FauI CCCGC 1 cut(s) 214
FbaI TGATCA 1 cut(s) 801
FokI GGATG 3 cut(s) 475, 628, 716
FriOI GRGCYC 2 cut(s) 395, 907
FspBI CTAG 6 cut(s) 54, 383, 612, 720, 2169, 2173
GlaI GCGC 1 cut(s) 576
GsaI CCCAGC 1 cut(s) 498
HaeIII GGCC 8 cut(s) 868, 1379, 1404, 1473, 1500, 1722, 1901, 2084
HapII CCGG 4 cut(s) 192, 1370, 2015, 2063
HgaI GACGC 2 cut(s) 327, 1774
HhaI GCGC 1 cut(s) 577
Hin1I GRCGYC 1 cut(s) 338
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HindIII AAGCTT 3 cut(s) 136, 1674, 1957
HinfI GANTC 7 cut(s) 59, 234, 454, 858, 933, 2045, 2144
HpaII CCGG 4 cut(s) 192, 1370, 2015, 2063
HphI GGTGA 5 cut(s) 796, 829, 989, 1119, 1700
Hpy166II GTNNAC 1 cut(s) 820
Hpy188I TCNGA 8 cut(s) 80, 703, 1186, 1350, 1546, 1751, 1890, 2128
Hpy188III TCNNGA 9 cut(s) 305, 646, 1237, 1450, 1534, 1564, 1621, 1936, 2163
Hpy8I GTNNAC 1 cut(s) 820
Hpy99I CGWCG 3 cut(s) 218, 343, 1214
HpyAV CCTTC 3 cut(s) 364, 1140, 1390
HpyCH4III ACNGT 4 cut(s) 118, 316, 484, 1733
HpyCH4IV ACGT 1 cut(s) 213
HpyF10VI GCNNNNNNNGC 5 cut(s) 617, 657, 660, 1502, 1849
HpyF3I CTNAG 6 cut(s) 146, 396, 408, 1191, 1196, 1227
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 1 cut(s) 338
HspAI GCGC 1 cut(s) 575
Ksp22I TGATCA 1 cut(s) 801
LmnI GCTCC 4 cut(s) 608, 902, 1372, 1931
LweI GCATC 4 cut(s) 159, 406, 1133, 2033
MaeI CTAG 6 cut(s) 54, 383, 612, 720, 2169, 2173
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 5 cut(s) 130, 941, 1786, 1834, 1853
MflI RGATCY 2 cut(s) 1872, 2165
MhlI GDGCHC 3 cut(s) 96, 395, 907
MlsI TGGCCA 2 cut(s) 1901, 2084
MluCI AATT 3 cut(s) 729, 1248, 1299
MluNI TGGCCA 2 cut(s) 1901, 2084
MlyI GAGTC 2 cut(s) 53, 2054
MmeI TCCRAC 1 cut(s) 1452
Mox20I TGGCCA 2 cut(s) 1901, 2084
Mph1103I ATGCAT 1 cut(s) 2108
MscI TGGCCA 2 cut(s) 1901, 2084
MseI TTAA 6 cut(s) 441, 1032, 1050, 1077, 1361, 1827
MslI CAYNNNNRTG 4 cut(s) 89, 827, 965, 2101
Msp20I TGGCCA 2 cut(s) 1901, 2084
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 4 cut(s) 192, 1370, 2015, 2063
MspR9I CCNGG 1 cut(s) 460
Mva1269I GAATGC 2 cut(s) 74, 1897
MvaI CCWGG 1 cut(s) 460
MwoI GCNNNNNNNGC 5 cut(s) 617, 657, 660, 1502, 1849
NcoI CCATGG 3 cut(s) 98, 1629, 1902
NlaIV GGNNCC 4 cut(s) 93, 1418, 1927, 2013
NmeAIII GCCGAG 1 cut(s) 1902
NmuCI GTSAC 2 cut(s) 130, 1786
NsiI ATGCAT 1 cut(s) 2108
NspI RCATGY 1 cut(s) 1948
OliI CACNNNNGTG 1 cut(s) 89
PciI ACATGT 1 cut(s) 1944
PctI GAATGC 2 cut(s) 74, 1897
PfeI GAWTC 5 cut(s) 234, 454, 858, 933, 2144
PflMI CCANNNNNTGG 1 cut(s) 734
PinAI ACCGGT 1 cut(s) 191
PleI GAGTC 2 cut(s) 53, 2053
PpsI GAGTC 2 cut(s) 53, 2053
PscI ACATGT 1 cut(s) 1944
Psp124BI GAGCTC 1 cut(s) 907
Psp6I CCWGG 1 cut(s) 458
PspFI CCCAGC 1 cut(s) 494
PspGI CCWGG 1 cut(s) 458
PspN4I GGNNCC 4 cut(s) 93, 1418, 1927, 2013
PspPI GGNCC 3 cut(s) 102, 1416, 1963
PstNI CAGNNNCTG 1 cut(s) 1190
PsuI RGATCY 2 cut(s) 1872, 2165
RsaI GTAC 4 cut(s) 1001, 1232, 1730, 1868
RsaNI GTAC 4 cut(s) 1000, 1231, 1729, 1867
RseI CAYNNNNRTG 4 cut(s) 89, 827, 965, 2101
SacI GAGCTC 1 cut(s) 907
SaqAI TTAA 6 cut(s) 441, 1032, 1050, 1077, 1361, 1827
Sau96I GGNCC 3 cut(s) 102, 1416, 1963
ScaI AGTACT 1 cut(s) 1232
SchI GAGTC 2 cut(s) 53, 2054
ScrFI CCNGG 1 cut(s) 460
SduI GDGCHC 3 cut(s) 96, 395, 907
SfaNI GCATC 4 cut(s) 159, 406, 1133, 2033
SfcI CTRYAG 2 cut(s) 1060, 1876
SgrAI CRCCGGYG 2 cut(s) 191, 2014
SinI GGWCC 3 cut(s) 102, 1416, 1963
SmiMI CAYNNNNRTG 4 cut(s) 89, 827, 965, 2101
SmlI CTYRAG 1 cut(s) 517
SmoI CTYRAG 1 cut(s) 517
Sse9I AATT 3 cut(s) 729, 1248, 1299
SsiI CCGC 7 cut(s) 221, 657, 1136, 1170, 1401, 2000, 2018
SspMI CTAG 6 cut(s) 54, 383, 612, 720, 2169, 2173
SstI GAGCTC 1 cut(s) 907
StyD4I CCNGG 1 cut(s) 458
StyI CCWWGG 4 cut(s) 98, 937, 1629, 1902
TaaI ACNGT 4 cut(s) 118, 316, 484, 1733
TaiI ACGT 1 cut(s) 216
TaqI TCGA 7 cut(s) 18, 62, 452, 506, 768, 1535, 1594
TasI AATT 3 cut(s) 729, 1248, 1299
TatI WGTACW 2 cut(s) 1230, 1866
TauI GCSGC 3 cut(s) 660, 1138, 1404
TfiI GAWTC 5 cut(s) 234, 454, 858, 933, 2144
Tru1I TTAA 6 cut(s) 441, 1032, 1050, 1077, 1361, 1827
Tru9I TTAA 6 cut(s) 441, 1032, 1050, 1077, 1361, 1827
TscAI CASTG 7 cut(s) 246, 262, 321, 367, 689, 1465, 1787
TseFI GTSAC 2 cut(s) 130, 1786
Tsp45I GTSAC 2 cut(s) 130, 1786
TspDTI ATGAA 6 cut(s) 154, 917, 1012, 1306, 2123, 2148
TspRI CASTG 7 cut(s) 246, 262, 321, 367, 689, 1465, 1787
Van91I CCANNNNNTGG 1 cut(s) 734
VpaK11BI GGWCC 3 cut(s) 102, 1416, 1963
XapI RAATTY 1 cut(s) 1248
XceI RCATGY 1 cut(s) 1948
XspI CTAG 6 cut(s) 54, 383, 612, 720, 2169, 2173
ZrmI AGTACT 1 cut(s) 1232
Zsp2I ATGCAT 1 cut(s) 2108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.