MD13G1011900.v1.1

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002592
Physical Location & Seq
Forward (+)
705687 .. 705965
279 bp
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UTR
Exon/CDS
Intron
MD13G1011900.v1.1.491

Sequence Viewer

Length: 279 bp
ATGACTAAGAGGACGAAGAAGGCCGGTATCGTTGGGAAATATGGAACCCGTTACGGTGCTAGTCTGCGTAAGCAGATTAAGAAAATGGAAGTCAGTCAGCACAGCAAGTATTTCTGTGAATTCTGTGGCAAGTATGCTGTGAAGAGAAAGGCTGTTGGAATCTGGGGATGTAAGGACTGCGGAAAAGTTAAGGCGGGCGGTGCCTACACCTTGAACACTGCTAGTGCTGTGACGGTTAGGAGCACCATTAGGAGGCTGAGGGAGCAAACCGAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L37ae

Protein Analysis

93

Amino Acids

10.26

Weight (kDa)

10.24

Isoelectric Point (pI)

31.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L37ae PF01780 4 - 88 1.6e-39 Ribosomal L37ae protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 200
AciI CCGC 3 cut(s) 180, 194, 198
AcsI RAATTY 1 cut(s) 119
AfiI CCNNNNNNNGG 1 cut(s) 252
AgsI TTSAA 1 cut(s) 214
Alw21I GWGCWC 1 cut(s) 245
AoxI GGCC 1 cut(s) 21
ApoI RAATTY 1 cut(s) 119
BanI GGYRCC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 245
BbvCI CCTCAGC 1 cut(s) 257
BfaI CTAG 2 cut(s) 60, 222
BmiI GGNNCC 2 cut(s) 46, 202
Bpu10I CCTNAGC 1 cut(s) 257
Bsc4I CCNNNNNNNGG 1 cut(s) 252
Bse118I RCCGGY 1 cut(s) 23
BseGI GGATG 1 cut(s) 173
BseLI CCNNNNNNNGG 1 cut(s) 252
BseMII CTCAG 1 cut(s) 248
BshFI GGCC 1 cut(s) 23
BshNI GGYRCC 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 245
BsiSI CCGG 1 cut(s) 24
BslI CCNNNNNNNGG 1 cut(s) 252
BsnI GGCC 1 cut(s) 23
Bsp1286I GDGCHC 1 cut(s) 245
BspACI CCGC 3 cut(s) 180, 194, 198
BspANI GGCC 1 cut(s) 23
BspCNI CTCAG 1 cut(s) 249
BspLI GGNNCC 2 cut(s) 46, 202
BspT107I GGYRCC 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 23
BssAI RCCGGY 1 cut(s) 23
Bst4CI ACNGT 2 cut(s) 56, 235
Bst6I CTCTTC 1 cut(s) 137
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 2 cut(s) 6, 257
BstF5I GGATG 1 cut(s) 173
BstMWI GCNNNNNNNGC 2 cut(s) 200, 262
BsuRI GGCC 1 cut(s) 23
BtsCI GGATG 1 cut(s) 173
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 1 cut(s) 216
Cac8I GCNNGC 1 cut(s) 196
Cfr10I RCCGGY 1 cut(s) 23
CviJI RGCY 3 cut(s) 23, 152, 256
CviKI_1 RGCY 3 cut(s) 23, 152, 256
DdeI CTNAG 2 cut(s) 6, 257
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 119
FaiI YATR 2 cut(s) 42, 135
FauI CCCGC 1 cut(s) 187
FokI GGATG 1 cut(s) 180
FspBI CTAG 2 cut(s) 60, 222
HaeIII GGCC 1 cut(s) 23
HapII CCGG 1 cut(s) 24
HinfI GANTC 1 cut(s) 159
HpaII CCGG 1 cut(s) 24
HpyAV CCTTC 1 cut(s) 13
HpyCH4III ACNGT 2 cut(s) 56, 235
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 262
HpyF3I CTNAG 2 cut(s) 6, 257
LmnI GCTCC 2 cut(s) 240, 262
LpnPI CCDG 2 cut(s) 37, 148
MaeI CTAG 2 cut(s) 60, 222
MaeIII GTNAC 2 cut(s) 50, 229
MboII GAAGA 2 cut(s) 28, 154
MhlI GDGCHC 1 cut(s) 245
MluCI AATT 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 136
MnlI CCTC 3 cut(s) 3, 246, 252
MseI TTAA 3 cut(s) 78, 189, 277
MspI CCGG 1 cut(s) 24
MwoI GCNNNNNNNGC 2 cut(s) 200, 262
NlaIV GGNNCC 2 cut(s) 46, 202
NmuCI GTSAC 1 cut(s) 229
PfeI GAWTC 1 cut(s) 159
PspN4I GGNNCC 2 cut(s) 46, 202
SaqAI TTAA 3 cut(s) 78, 189, 277
SduI GDGCHC 1 cut(s) 245
SetI ASST 1 cut(s) 212
SgeI CNNG 9 cut(s) 36, 60, 72, 118, 142, 175, 207, 223, 234
Sse9I AATT 1 cut(s) 119
SsiI CCGC 3 cut(s) 180, 194, 198
SspMI CTAG 2 cut(s) 60, 222
TaaI ACNGT 2 cut(s) 56, 235
TasI AATT 1 cut(s) 119
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 3 cut(s) 78, 189, 277
Tru9I TTAA 3 cut(s) 78, 189, 277
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 229
TspRI CASTG 1 cut(s) 223
XapI RAATTY 1 cut(s) 119
XspI CTAG 2 cut(s) 60, 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.