Rroxscaffold_3G00251480

60S ribosomal protein L37a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002592
Physical Location & Seq
Forward (+)
45354965 .. 45361128
6164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00251480.1

Sequence Viewer

Length: 525 bp
ATGAAGAATTTGACTCTGTCCTCATGTCTGAAGGGATTGGTTCCACTGATTCTATTGTGGTTGAAACTAATGACTCAATCCCTCCCGGTAAGGAAGTTACTCAGAACATGCTTTGTAGTCAAGGGGGAACATCGTCTTAAACTTGATGAAGAAATTGGATGGATTTGCGAAGTCTGCCCTTATGTGCATCAGGAGATCAAGTCTATTATACCAGGCTTTGCTCCAAACCCCTATGGAAAAGCTGGGAAGAGATTTGATGAAGAACACAACCGGGTTGATGATCTTCAGGGTAATGACCTTGACGATTCTGGCTTTGGTTCCCAAACTAAGAGAACGAAGAAGGCTGGAATCGTCGGGAAATATGGTACCCGTTATGGTGCCAGTCTGCGTAAGCAGATTAAGAAGATGGAAGTCAGTCAGCACAGCAAGTATTTCTGTGAATTCTGCGGCAAGGTAACGCGCACTGCTGCAGCCGTTACAGTTAGAAGCACCATCAGGAGGCTGAGGGAGCAAACTGAGAGTTAA

Protein Analysis

174

Amino Acids

19.76

Weight (kDa)

9.65

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L37ae PF01780 111 - 152 1.6e-17 Ribosomal L37ae protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 365
AccB1I GGYRCC 2 cut(s) 365, 377
AccII CGCG 1 cut(s) 460
AciI CCGC 1 cut(s) 447
AcsI RAATTY 2 cut(s) 7, 440
AcuI CTGAAG 2 cut(s) 50, 269
AfaI GTAC 1 cut(s) 367
AfiI CCNNNNNNNGG 1 cut(s) 498
AgsI TTSAA 1 cut(s) 64
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 1 cut(s) 242
AluI AGCT 1 cut(s) 242
ApeKI GCWGC 2 cut(s) 467, 470
ApoI RAATTY 2 cut(s) 7, 440
Asp718I GGTACC 1 cut(s) 365
AspLEI GCGC 1 cut(s) 462
AsuC2I CCSGG 2 cut(s) 86, 272
BanI GGYRCC 2 cut(s) 365, 377
BbvCI CCTCAGC 1 cut(s) 503
BbvI GCAGC 2 cut(s) 454, 482
BccI CCATC 3 cut(s) 153, 400, 500
BceAI ACGGC 1 cut(s) 458
BciT130I CCWGG 1 cut(s) 213
BcnI CCSGG 2 cut(s) 86, 272
BfmI CTRYAG 1 cut(s) 468
BisI GCNGC 3 cut(s) 448, 468, 471
BlsI GCNGC 3 cut(s) 449, 469, 472
Bme1390I CCNGG 3 cut(s) 86, 213, 272
BmiI GGNNCC 4 cut(s) 42, 319, 367, 379
BmrFI CCNGG 3 cut(s) 86, 213, 272
BmsI GCATC 1 cut(s) 196
Bpu10I CCTNAGC 1 cut(s) 503
BpuMI CCSGG 2 cut(s) 86, 272
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 1 cut(s) 498
Bse1I ACTGG 1 cut(s) 381
BseBI CCWGG 1 cut(s) 213
BseGI GGATG 1 cut(s) 164
BseLI CCNNNNNNNGG 1 cut(s) 498
BseMII CTCAG 3 cut(s) 115, 494, 507
BseNI ACTGG 1 cut(s) 381
BseXI GCAGC 2 cut(s) 454, 482
BseYI CCCAGC 1 cut(s) 242
Bsh1236I CGCG 1 cut(s) 460
BshNI GGYRCC 2 cut(s) 365, 377
BsiSI CCGG 2 cut(s) 86, 271
BslI CCNNNNNNNGG 1 cut(s) 498
Bsp143I GATC 2 cut(s) 195, 280
BspACI CCGC 1 cut(s) 447
BspCNI CTCAG 3 cut(s) 114, 495, 508
BspFNI CGCG 1 cut(s) 460
BspLI GGNNCC 4 cut(s) 42, 319, 367, 379
BspMAI CTGCAG 1 cut(s) 472
BspT107I GGYRCC 2 cut(s) 365, 377
BsrI ACTGG 1 cut(s) 381
BssMI GATC 2 cut(s) 195, 280
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 1 cut(s) 481
Bst6I CTCTTC 1 cut(s) 242
BstDEI CTNAG 4 cut(s) 101, 327, 503, 516
BstF5I GGATG 1 cut(s) 164
BstFNI CGCG 1 cut(s) 460
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 2 cut(s) 198, 283
BstMBI GATC 2 cut(s) 195, 280
BstMWI GCNNNNNNNGC 2 cut(s) 174, 508
BstNI CCWGG 1 cut(s) 213
BstNSI RCATGY 1 cut(s) 111
BstSCI CCNGG 3 cut(s) 84, 211, 270
BstSFI CTRYAG 1 cut(s) 468
BstUI CGCG 1 cut(s) 460
BstV1I GCAGC 2 cut(s) 454, 482
BtsCI GGATG 1 cut(s) 164
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 2 cut(s) 44, 462
CfoI GCGC 1 cut(s) 462
Csp6I GTAC 1 cut(s) 366
CviAII CATG 2 cut(s) 24, 108
CviJI RGCY 6 cut(s) 216, 242, 312, 344, 473, 502
CviKI_1 RGCY 6 cut(s) 216, 242, 312, 344, 473, 502
CviQI GTAC 1 cut(s) 366
DdeI CTNAG 4 cut(s) 101, 327, 503, 516
DpnI GATC 2 cut(s) 197, 282
DpnII GATC 2 cut(s) 195, 280
Eam1104I CTCTTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 242
Eco57I CTGAAG 2 cut(s) 50, 269
EcoRI GAATTC 1 cut(s) 440
EcoRII CCWGG 1 cut(s) 211
FaeI CATG 2 cut(s) 27, 111
FaiI YATR 7 cut(s) 25, 109, 183, 209, 234, 363, 375
FatI CATG 2 cut(s) 23, 107
Fnu4HI GCNGC 3 cut(s) 448, 468, 471
FokI GGATG 1 cut(s) 171
Fsp4HI GCNGC 3 cut(s) 448, 468, 471
GlaI GCGC 1 cut(s) 461
GluI GCNGC 3 cut(s) 448, 468, 471
GsaI CCCAGC 1 cut(s) 246
HapII CCGG 2 cut(s) 86, 271
HhaI GCGC 1 cut(s) 462
Hin1II CATG 2 cut(s) 27, 111
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HinfI GANTC 5 cut(s) 13, 49, 73, 305, 348
HpaII CCGG 2 cut(s) 86, 271
Hpy188I TCNGA 2 cut(s) 30, 104
Hpy188III TCNNGA 3 cut(s) 191, 355, 496
Hpy99I CGWCG 1 cut(s) 356
HpyAV CCTTC 2 cut(s) 25, 334
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4V TGCA 2 cut(s) 187, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 174, 508
HpyF3I CTNAG 4 cut(s) 101, 327, 503, 516
Hsp92II CATG 2 cut(s) 27, 111
HspAI GCGC 1 cut(s) 460
KpnI GGTACC 1 cut(s) 369
Kzo9I GATC 2 cut(s) 195, 280
LmnI GCTCC 2 cut(s) 226, 508
Lsp1109I GCAGC 2 cut(s) 454, 482
LweI GCATC 1 cut(s) 196
MaeIII GTNAC 3 cut(s) 96, 454, 475
MalI GATC 2 cut(s) 197, 282
MboI GATC 2 cut(s) 195, 280
MboII GAAGA 7 cut(s) 16, 161, 259, 272, 275, 349, 415
MluCI AATT 3 cut(s) 7, 153, 440
MlyI GAGTC 2 cut(s) 7, 67
MnlI CCTC 4 cut(s) 31, 92, 492, 498
MseI TTAA 3 cut(s) 138, 399, 523
MspI CCGG 2 cut(s) 86, 271
MspR9I CCNGG 3 cut(s) 86, 213, 272
MvaI CCWGG 1 cut(s) 213
MvnI CGCG 1 cut(s) 460
MwoI GCNNNNNNNGC 2 cut(s) 174, 508
NciI CCSGG 2 cut(s) 86, 272
NdeII GATC 2 cut(s) 195, 280
NlaIII CATG 2 cut(s) 27, 111
NlaIV GGNNCC 4 cut(s) 42, 319, 367, 379
NspI RCATGY 1 cut(s) 111
PfeI GAWTC 3 cut(s) 49, 305, 348
PflFI GACNNNGTC 1 cut(s) 16
PkrI GCNGC 3 cut(s) 449, 469, 472
PleI GAGTC 2 cut(s) 7, 67
PpsI GAGTC 2 cut(s) 7, 67
Psp6I CCWGG 1 cut(s) 211
PspFI CCCAGC 1 cut(s) 242
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 4 cut(s) 42, 319, 367, 379
PstI CTGCAG 1 cut(s) 472
PsyI GACNNNGTC 1 cut(s) 16
RsaI GTAC 1 cut(s) 367
RsaNI GTAC 1 cut(s) 366
SaqAI TTAA 3 cut(s) 138, 399, 523
SatI GCNGC 3 cut(s) 448, 468, 471
Sau3AI GATC 2 cut(s) 195, 280
SchI GAGTC 2 cut(s) 7, 67
ScrFI CCNGG 3 cut(s) 86, 213, 272
SetI ASST 3 cut(s) 244, 300, 456
SfaNI GCATC 1 cut(s) 196
SfcI CTRYAG 1 cut(s) 468
Sse9I AATT 3 cut(s) 7, 153, 440
SsiI CCGC 1 cut(s) 447
StyD4I CCNGG 3 cut(s) 84, 211, 270
TaaI ACNGT 1 cut(s) 481
TasI AATT 3 cut(s) 7, 153, 440
TauI GCSGC 1 cut(s) 450
TfiI GAWTC 3 cut(s) 49, 305, 348
Tru1I TTAA 3 cut(s) 138, 399, 523
Tru9I TTAA 3 cut(s) 138, 399, 523
TscAI CASTG 2 cut(s) 51, 469
TseI GCWGC 2 cut(s) 467, 470
TspDTI ATGAA 3 cut(s) 17, 162, 273
TspRI CASTG 2 cut(s) 51, 469
Tth111I GACNNNGTC 1 cut(s) 16
XapI RAATTY 2 cut(s) 7, 440
XceI RCATGY 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.