Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002566

Physical Location & Seq

Genomic Coordinates Forward (+)

50298381 .. 50306000

Gene Structure 7620 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1402100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 669 bp

ATGATTCTCCCCTCGTTAGAAAAGGTGGGCACTCTAGACGCGCAGAGAGTGGTTCATCAACACCAAGGATGGCGGGTGATCTCATGTATGTGGTTACATGCTGGAGTTTTCCATGTGCTTGCCAATATGCTGAGTCTTGTATTCATTGGAATTCGGCTTGAGCAAGAATTTGGATTTGTTCGAATTGGTTTCCTATATCTCATGTCTGGTTTTGGTGGGAGTTTGCTTTCAGCTCTTTTTATTCAATATGGTATTTCTGTTGGTGCTTCTGGTGCACTTTTTGGTTTACTAGGAAGCATGCTTTCAGAGCTCACATCAAATTGGACAATGTATGTAAATAAGTTTCAGATTTCGATCCGCATGGGAATTCTACCACACGTGGACAACTTTGCTCATATTGGAGGATTTGTCTCTGGATTTCTTCTTGGATTTTTGTTTTTGATCTGCCCCCAGTTTAAATGGCTTACCCAACGGAATGCTCCCCCTGGACGTGTTACTACTCCAGTGAAATCTAAACACAAGACGTATCAATATGTATTGTGGGTCCTCTCTTTGATACTATTGATTGTTGGGTATACTCTTGGCTTGGTTGCTCTTTTTCGAGGGTTGAGCCAGATAGGGAATCAGCTGAACTTAACGTGCGTAAGCAATGGTAGAAATGGGACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0002791 GO:0002792 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0006928 GO:0008150 GO:0008283 GO:0009894 GO:0009966 GO:0009987 GO:0010646 GO:0012505 GO:0016020 GO:0016477 GO:0019222 GO:0023051 GO:0030162 GO:0031090 GO:0031323 GO:0031329 GO:0031984 GO:0032268 GO:0032879 GO:0032880 GO:0040011 GO:0042058 GO:0042175 GO:0042176 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048870 GO:0050708 GO:0050709 GO:0050789 GO:0050794 GO:0051046 GO:0051048 GO:0051049 GO:0051051 GO:0051171 GO:0051179 GO:0051223 GO:0051224 GO:0051246 GO:0051674 GO:0060255 GO:0061136 GO:0065007 GO:0070201 GO:0080090 GO:0090087 GO:0098588 GO:0098791 GO:0098827 GO:1901184 GO:1903050 GO:1903362 GO:1903530 GO:1903531 GO:1904950

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rhomboid

Protein Analysis

223

Amino Acids

24.57

Weight (kDa)

9.89

Isoelectric Point (pI)

23.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rhomboid	PF01694	21 - 147	1.8e-34	Rhomboid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002566)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G29050 AT2G29050
fragaria_vesca	FvH4_3g36420 FvH4_3g36420 FvH4_3g36420 FvH4_3g36420
malus_domestica	MD00G1013900.v1.1 MD00G1178600.v1.1 MD00G1178800.v1.1 MD03G1090500.v1.1 MD11G1100200.v1.1 MD11G1101200.v1.1 MD11G1101800.v1.1 MD11G1102100.v1.1 MD11G1104300.v1.1 MD11G1105000.v1.1 MD11G1106000.v1.1 MD11G1106300.v1.1 MD11G1106700.v1.1 MD11G1106900.v1.1 MD11G1107000.v1.1 MD11G1107900.v1.1 MD11G1261200.v1.1 MD15G1402100.v1.1
prunus_persica	Prupe.6G074600_v2.0.a1
pyrus_communis	pycom03g07200 pycom11g08440
rosa_chinensis	RchiOBHm_Chr5g0065311
rosa_laevigata	RLG00000035780 RLG00000035781 RLG00000035782
rosa_multiflora	Rmu_sc0009942.1_g000006 Rmu_sc0013827.1_g000001
rosa_roxburghii	Rroxscaffold_1G00015870
rosa_rugosa	Rorug05G0371200
rosa_samantha	Rh5AG429000 Rh5BG444300 Rh5CG466500 Rh5DG456600
rosa_wichuraiana	Rw5G040270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	575
AccII	CGCG	1 cut(s)	41
AciI	CCGC	2 cut(s)	73, 358
AclWI	GGATC	1 cut(s)	349
AcsI	RAATTY	3 cut(s)	150, 167, 366
AcvI	CACGTG	1 cut(s)	379
AflIII	ACRYGT	2 cut(s)	376, 490
AgsI	TTSAA	1 cut(s)	245
AjiI	CACGTC	1 cut(s)	491
AjnI	CCWGG	1 cut(s)	484
AluBI	AGCT	3 cut(s)	233, 310, 628
AluI	AGCT	3 cut(s)	233, 310, 628
Alw21I	GWGCWC	2 cut(s)	277, 312
Alw26I	GTCTC	1 cut(s)	415
Alw44I	GTGCAC	1 cut(s)	273
AlwI	GGATC	1 cut(s)	349
ApaLI	GTGCAC	1 cut(s)	273
ApoI	RAATTY	3 cut(s)	150, 167, 366
AspLEI	GCGC	1 cut(s)	43
AspS9I	GGNCC	1 cut(s)	544
AsuHPI	GGTGA	1 cut(s)	88
AsuII	TTCGAA	1 cut(s)	181
AvaII	GGWCC	1 cut(s)	544
BaeGI	GKGCMC	2 cut(s)	32, 277
BanII	GRGCYC	1 cut(s)	312
BbrPI	CACGTG	1 cut(s)	379
Bbv12I	GWGCWC	2 cut(s)	277, 312
BccI	CCATC	1 cut(s)	63
BciT130I	CCWGG	1 cut(s)	486
BcoDI	GTCTC	1 cut(s)	415
BfaI	CTAG	2 cut(s)	35, 290
Bme1390I	CCNGG	1 cut(s)	486
Bme18I	GGWCC	1 cut(s)	544
BmgBI	CACGTC	1 cut(s)	491
BmgT120I	GGNCC	1 cut(s)	544
BmiI	GGNNCC	1 cut(s)	545
BmrFI	CCNGG	1 cut(s)	486
BmrI	ACTGGG	1 cut(s)	445
BmuI	ACTGGG	1 cut(s)	445
BpmI	CTGGAG	2 cut(s)	123, 486
Bpu14I	TTCGAA	1 cut(s)	181
BpuEI	CTTGAG	1 cut(s)	179
BsaAI	YACGTR	1 cut(s)	379
BsaBI	GATNNNNATC	1 cut(s)	353
BsaJI	CCNNGG	2 cut(s)	64, 484
BsaXI	ACNNNNNCTCC	2 cut(s)	393, 423
Bse1I	ACTGG	2 cut(s)	451, 503
Bse3DI	GCAATG	1 cut(s)	655
Bse8I	GATNNNNATC	1 cut(s)	353
BseBI	CCWGG	1 cut(s)	486
BseDI	CCNNGG	2 cut(s)	64, 484
BseGI	GGATG	1 cut(s)	74
BseJI	GATNNNNATC	1 cut(s)	353
BseMI	GCAATG	1 cut(s)	655
BseMII	CTCAG	1 cut(s)	122
BseNI	ACTGG	2 cut(s)	451, 503
BseSI	GKGCMC	2 cut(s)	32, 277
Bsh1236I	CGCG	1 cut(s)	41
BsiHKAI	GWGCWC	2 cut(s)	277, 312
BsmAI	GTCTC	1 cut(s)	415
BsmI	GAATGC	1 cut(s)	481
Bsp119I	TTCGAA	1 cut(s)	181
Bsp1286I	GDGCHC	3 cut(s)	32, 277, 312
Bsp143I	GATC	3 cut(s)	78, 354, 441
BspACI	CCGC	2 cut(s)	73, 358
BspCNI	CTCAG	1 cut(s)	123
BspFNI	CGCG	1 cut(s)	41
BspLI	GGNNCC	1 cut(s)	545
BspPI	GGATC	1 cut(s)	349
BspT104I	TTCGAA	1 cut(s)	181
BsrDI	GCAATG	1 cut(s)	655
BsrI	ACTGG	2 cut(s)	451, 503
BssECI	CCNNGG	2 cut(s)	64, 484
BssMI	GATC	3 cut(s)	78, 354, 441
BssNAI	GTATAC	1 cut(s)	576
BssT1I	CCWWGG	1 cut(s)	64
Bst1107I	GTATAC	1 cut(s)	576
Bst2UI	CCWGG	1 cut(s)	486
BstBAI	YACGTR	1 cut(s)	379
BstBI	TTCGAA	1 cut(s)	181
BstC8I	GCNNGC	2 cut(s)	120, 299
BstDEI	CTNAG	1 cut(s)	131
BstF5I	GGATG	1 cut(s)	74
BstFNI	CGCG	1 cut(s)	41
BstHHI	GCGC	1 cut(s)	43
BstKTI	GATC	3 cut(s)	81, 357, 444
BstMAI	GTCTC	1 cut(s)	415
BstMBI	GATC	3 cut(s)	78, 354, 441
BstMWI	GCNNNNNNNGC	2 cut(s)	272, 307
BstNI	CCWGG	1 cut(s)	486
BstNSI	RCATGY	2 cut(s)	101, 301
BstSCI	CCNGG	1 cut(s)	484
BstSLI	GKGCMC	2 cut(s)	32, 277
BstUI	CGCG	1 cut(s)	41
BstZ17I	GTATAC	1 cut(s)	576
BtrI	CACGTC	1 cut(s)	491
BtsCI	GGATG	1 cut(s)	74
BtsIMutI	CAGTG	1 cut(s)	510
Cac8I	GCNNGC	2 cut(s)	120, 299
CfoI	GCGC	1 cut(s)	43
Cfr13I	GGNCC	1 cut(s)	544
CseI	GACGC	1 cut(s)	47
CviAII	CATG	6 cut(s)	84, 98, 113, 202, 298, 361
CviJI	RGCY	7 cut(s)	157, 233, 310, 463, 585, 612, 628
CviKI_1	RGCY	7 cut(s)	157, 233, 310, 463, 585, 612, 628
DdeI	CTNAG	1 cut(s)	131
DpnI	GATC	3 cut(s)	80, 356, 443
DpnII	GATC	3 cut(s)	78, 354, 441
DraI	TTTAAA	1 cut(s)	457
Ecl136II	GAGCTC	1 cut(s)	310
Eco130I	CCWWGG	1 cut(s)	64
Eco24I	GRGCYC	1 cut(s)	312
Eco47I	GGWCC	1 cut(s)	544
Eco53kI	GAGCTC	1 cut(s)	310
Eco72I	CACGTG	1 cut(s)	379
EcoICRI	GAGCTC	1 cut(s)	310
EcoO109I	RGGNCCY	1 cut(s)	544
EcoRI	GAATTC	2 cut(s)	150, 366
EcoRII	CCWGG	1 cut(s)	484
EcoT14I	CCWWGG	1 cut(s)	64
EcoT38I	GRGCYC	1 cut(s)	312
ErhI	CCWWGG	1 cut(s)	64
FaeI	CATG	6 cut(s)	87, 101, 116, 205, 301, 364
FatI	CATG	6 cut(s)	83, 97, 112, 201, 297, 360
FauI	CCCGC	1 cut(s)	66
FblI	GTMKAC	1 cut(s)	575
FokI	GGATG	1 cut(s)	81
FriOI	GRGCYC	1 cut(s)	312
FspBI	CTAG	2 cut(s)	35, 290
GlaI	GCGC	1 cut(s)	42
GsuI	CTGGAG	2 cut(s)	123, 486
HgaI	GACGC	1 cut(s)	47
HhaI	GCGC	1 cut(s)	43
Hin1II	CATG	6 cut(s)	87, 101, 116, 205, 301, 364
Hin6I	GCGC	1 cut(s)	41
HinP1I	GCGC	1 cut(s)	41
HinfI	GANTC	3 cut(s)	4, 133, 622
HphI	GGTGA	1 cut(s)	88
Hpy166II	GTNNAC	4 cut(s)	275, 287, 382, 576
Hpy188I	TCNGA	2 cut(s)	307, 348
Hpy188III	TCNNGA	2 cut(s)	35, 414
Hpy8I	GTNNAC	4 cut(s)	275, 287, 382, 576
HpyCH4IV	ACGT	4 cut(s)	378, 490, 524, 638
HpyCH4V	TGCA	1 cut(s)	275
HpyF10VI	GCNNNNNNNGC	2 cut(s)	272, 307
HpyF3I	CTNAG	1 cut(s)	131
HpySE526I	ACGT	4 cut(s)	378, 490, 524, 638
Hsp92II	CATG	6 cut(s)	87, 101, 116, 205, 301, 364
HspAI	GCGC	1 cut(s)	41
Kzo9I	GATC	3 cut(s)	78, 354, 441
LmnI	GCTCC	1 cut(s)	484
LpnPI	CCDG	9 cut(s)	87, 192, 255, 399, 464, 471, 498, 516, 626
MaeI	CTAG	2 cut(s)	35, 290
MaeII	ACGT	4 cut(s)	378, 490, 524, 638
MaeIII	GTNAC	2 cut(s)	93, 493
MalI	GATC	3 cut(s)	80, 356, 443
MboI	GATC	3 cut(s)	78, 354, 441
MboII	GAAGA	1 cut(s)	413
MhlI	GDGCHC	3 cut(s)	32, 277, 312
MluCI	AATT	5 cut(s)	150, 167, 183, 319, 366
MlyI	GAGTC	1 cut(s)	142
MnlI	CCTC	4 cut(s)	22, 395, 557, 596
MseI	TTAA	2 cut(s)	456, 635
MslI	CAYNNNNRTG	1 cut(s)	88
MspA1I	CMGCKG	1 cut(s)	628
MspR9I	CCNGG	1 cut(s)	486
Mva1269I	GAATGC	1 cut(s)	481
MvaI	CCWGG	1 cut(s)	486
MvnI	CGCG	1 cut(s)	41
MwoI	GCNNNNNNNGC	2 cut(s)	272, 307
NdeII	GATC	3 cut(s)	78, 354, 441
NlaIII	CATG	6 cut(s)	87, 101, 116, 205, 301, 364
NlaIV	GGNNCC	1 cut(s)	545
NspI	RCATGY	2 cut(s)	101, 301
NspV	TTCGAA	1 cut(s)	181
PaeI	GCATGC	1 cut(s)	301
PctI	GAATGC	1 cut(s)	481
PfeI	GAWTC	2 cut(s)	4, 622
PleI	GAGTC	1 cut(s)	141
PmaCI	CACGTG	1 cut(s)	379
PmlI	CACGTG	1 cut(s)	379
PpsI	GAGTC	1 cut(s)	141
Ppu21I	YACGTR	1 cut(s)	379
PpuMI	RGGWCCY	1 cut(s)	544
Psp124BI	GAGCTC	1 cut(s)	312
Psp5II	RGGWCCY	1 cut(s)	544
Psp6I	CCWGG	1 cut(s)	484
PspCI	CACGTG	1 cut(s)	379
PspGI	CCWGG	1 cut(s)	484
PspN4I	GGNNCC	1 cut(s)	545
PspPI	GGNCC	1 cut(s)	544
PspPPI	RGGWCCY	1 cut(s)	544
PvuII	CAGCTG	1 cut(s)	628
RseI	CAYNNNNRTG	1 cut(s)	88
SacI	GAGCTC	1 cut(s)	312
SaqAI	TTAA	2 cut(s)	456, 635
Sau3AI	GATC	3 cut(s)	78, 354, 441
Sau96I	GGNCC	1 cut(s)	544
SchI	GAGTC	1 cut(s)	142
ScrFI	CCNGG	1 cut(s)	486
SduI	GDGCHC	3 cut(s)	32, 277, 312
SetI	ASST	8 cut(s)	27, 235, 312, 381, 493, 527, 630, 641
SfuI	TTCGAA	1 cut(s)	181
SinI	GGWCC	1 cut(s)	544
SmiMI	CAYNNNNRTG	1 cut(s)	88
SmlI	CTYRAG	1 cut(s)	158
SmoI	CTYRAG	1 cut(s)	158
SphI	GCATGC	1 cut(s)	301
Sse9I	AATT	5 cut(s)	150, 167, 183, 319, 366
SsiI	CCGC	2 cut(s)	73, 358
SspMI	CTAG	2 cut(s)	35, 290
SstI	GAGCTC	1 cut(s)	312
StyD4I	CCNGG	1 cut(s)	484
StyI	CCWWGG	1 cut(s)	64
TaiI	ACGT	4 cut(s)	381, 493, 527, 641
TaqI	TCGA	3 cut(s)	181, 353, 601
TasI	AATT	5 cut(s)	150, 167, 183, 319, 366
TfiI	GAWTC	2 cut(s)	4, 622
Tru1I	TTAA	2 cut(s)	456, 635
Tru9I	TTAA	2 cut(s)	456, 635
TscAI	CASTG	1 cut(s)	510
TspDTI	ATGAA	2 cut(s)	44, 133
TspGWI	ACGGA	1 cut(s)	487
TspRI	CASTG	1 cut(s)	510
VneI	GTGCAC	1 cut(s)	273
VpaK11BI	GGWCC	1 cut(s)	544
XapI	RAATTY	3 cut(s)	150, 167, 366
XbaI	TCTAGA	1 cut(s)	34
XceI	RCATGY	2 cut(s)	101, 301
XmiI	GTMKAC	1 cut(s)	575
XspI	CTAG	2 cut(s)	35, 290

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.