Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001381

Physical Location & Seq

Genomic Coordinates Forward (+)

1067882 .. 1071207

Gene Structure 3326 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1013900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 837 bp

ATGGCGGCCACAGTGACCTCTCAGTCCTCTCTCATACAATCAAAACCCGCTCTCTCTTCAAAAACCCTGCAAAACCCTCGTCCTACCTCCTTTGCCGTGCCCTCCAATTCCCTTTCACGACTGCAATTTGGTGTGAAATTGGGAAATGGGTTCGCCGCAAAAGCTTCAATGGCCGGCTCCGAACAGAACTCGAAGCCCCCCGTTGCCGAAAAGAGGGTCAAGAATCCCATCATTGTCATTGACAATTATGATAGCTTTACCTACAATCTCTGCCAGTATATGGGTGAGTTGGGATGTCTCTTTGAAGTCTATCGGAACGATGAACTAAGTGTTGATGAACTAAAAAGGAAAAATCCCAGAGGAGTGCTAATCTCTCCTGGGCCAGGCACCCCTCAAGATTCTGGAATATCGTTGCAAACTGTGTTGGAGCTAGGGCCCATTGTACCCTTATTCGGTGTATGTATGGGTTTGCAGTGCATCGGGGAGGCTTTTGGAGGGAAAATTGTTCGTTCTCCATTTGGCGTCATGCATGGGAAGAGCTCTCCTGTATATTATAACGAGGAGGAGGAAGATAGCTTGTTTTCGGGGTTAACAAACCCTTTCACGGCTGGCAGATATCACAGTCTTGTAATCGATAAGGACAGTTTCCCAGGTGAAGAACTGGAAATTACAGCATGGACAGAGGATGGACTGATAATGGCTGCTCGTCACAAGAAATATAAGCATCTTCAAGGGGTTCAGTTCCATCCTGAAAGTATCATAACTTCTGAAGGCAAGACAATTGTGTGTAACTTTATTAAATCGATAGAGAAAAAAGAGTCTGAATCTGAGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000162 GO:0003674 GO:0003824 GO:0004049 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005950 GO:0006082 GO:0006520 GO:0006568 GO:0006576 GO:0006586 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009308 GO:0009309 GO:0009889 GO:0009987 GO:0010600 GO:0010817 GO:0016053 GO:0016829 GO:0016830 GO:0016833 GO:0018130 GO:0019222 GO:0019438 GO:0019752 GO:0031323 GO:0031326 GO:0032350 GO:0032991 GO:0034641 GO:0042401 GO:0042430 GO:0042435 GO:0043436 GO:0044106 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046219 GO:0046394 GO:0046483 GO:0046885 GO:0050789 GO:0050794 GO:0065007 GO:0065008 GO:0071704 GO:0090354 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00400 ko00405 ko01100 ko01110 ko01130 ko01230 ko02024 ko02025 map00400 map00405 map01100 map01110 map01130 map01230 map02024 map02025

Pfam Domains

Protein Families

GATase

Protein Analysis

279

Amino Acids

30.37

Weight (kDa)

6.01

Isoelectric Point (pI)

55.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase	PF00117	79 - 266	1.6e-52	Glutamine amidotransferase class-I
Peptidase_C26	PF07722	148 - 251	2.1e-11	Peptidase C26

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001381)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24807 AT1G24909 AT1G25083 AT1G25155 AT1G25220 AT1G25220 AT5G57890
fragaria_vesca	FvH4_4g33060
malus_domestica	MD13G1016400.v1.1 MD16G1013900.v1.1
prunus_persica	Prupe.1G337100_v2.0.a1
pyrus_communis	pycom13g01410 pycom16g01310
rosa_chinensis	RchiOBHm_Chr3g0486701 RchiOBHm_Chr3g0486721 RchiOBHm_Chr4g0388381 RchiOBHm_Chr4g0388411 RchiOBHm_Chr4g0388431 RchiOBHm_Chr4g0442101 RchiOBHm_Chr4g0442111
rosa_laevigata	RLG00000010056
rosa_multiflora	Rmu_sc0000753.1_g000008 Rmu_sc0001949.1_g000032 Rmu_sc0001949.1_g000033 Rmu_sc0002501.1_g000003 Rmu_sc0015680.1_g000001 Rmu_sc0018709.1_g000002 Rmu_sc0018709.1_g000003
rosa_roxburghii	Rroxscaffold_4G00314730 Rroxscaffold_4G00314740 Rroxscaffold_4G00314750 Rroxscaffold_4G00314770 Rroxscaffold_4G00314790 Rroxscaffold_4G00314820 Rroxscaffold_5G00334930 Rroxscaffold_5G00383020
rosa_rugosa	Rorug04G0338900 Rorug04G0338900 Rorug04G0339000
rosa_samantha	Rh3AG276100 Rh3AG276300 Rh4AG022400 Rh4AG390400 Rh4BG016000 Rh4BG016100 Rh4BG016300 Rh4BG404000 Rh4CG023200 Rh4CG023400 Rh4CG419000 Rh4DG397000
rosa_wichuraiana	Rw4G001550 Rw4G033740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	555
AasI	GACNNNNNNGTC	1 cut(s)	22
AccB1I	GGYRCC	1 cut(s)	386
AccB7I	CCANNNNNTGG	1 cut(s)	280
AccBSI	CCGCTC	1 cut(s)	50
AciI	CCGC	3 cut(s)	5, 48, 156
AcoI	YGGCCR	2 cut(s)	6, 171
AcuI	CTGAAG	1 cut(s)	789
AcyI	GRCGYC	1 cut(s)	522
AfaI	GTAC	1 cut(s)	444
AfiI	CCNNNNNNNGG	5 cut(s)	213, 280, 383, 452, 604
AgsI	TTSAA	4 cut(s)	60, 168, 305, 731
AjnI	CCWGG	3 cut(s)	376, 382, 649
AluBI	AGCT	5 cut(s)	164, 255, 430, 540, 576
AluI	AGCT	5 cut(s)	164, 255, 430, 540, 576
Alw21I	GWGCWC	1 cut(s)	542
Alw26I	GTCTC	1 cut(s)	302
AoxI	GGCC	4 cut(s)	6, 171, 380, 434
ApaI	GGGCCC	1 cut(s)	438
ApeKI	GCWGC	1 cut(s)	701
ArsI	GACNNNNNNTTYG	4 cut(s)	64, 96, 201, 233
AspS9I	GGNCC	3 cut(s)	380, 434, 435
AsuHPI	GGTGA	2 cut(s)	296, 665
BaeGI	GKGCMC	2 cut(s)	102, 438
BanI	GGYRCC	1 cut(s)	386
BanII	GRGCYC	2 cut(s)	438, 542
BarI	GAAGNNNNNNTAC	2 cut(s)	748, 780
Bbv12I	GWGCWC	1 cut(s)	542
BbvI	GCAGC	1 cut(s)	688
BccI	CCATC	3 cut(s)	236, 680, 753
BceAI	ACGGC	2 cut(s)	80, 621
BcgI	CGANNNNNNTGC	2 cut(s)	59, 93
BciT130I	CCWGG	3 cut(s)	378, 384, 651
BcoDI	GTCTC	1 cut(s)	302
BfaI	CTAG	1 cut(s)	431
BisI	GCNGC	3 cut(s)	6, 156, 702
BlsI	GCNGC	3 cut(s)	7, 157, 703
Bme1390I	CCNGG	3 cut(s)	378, 384, 651
BmgT120I	GGNCC	3 cut(s)	380, 434, 435
BmiI	GGNNCC	3 cut(s)	178, 388, 436
BmrFI	CCNGG	3 cut(s)	378, 384, 651
BmsI	GCATC	2 cut(s)	486, 733
BpuEI	CTTGAG	1 cut(s)	378
Bsa29I	ATCGAT	2 cut(s)	633, 803
BsaHI	GRCGYC	1 cut(s)	522
BsaJI	CCNNGG	2 cut(s)	377, 649
Bsc4I	CCNNNNNNNGG	5 cut(s)	213, 280, 383, 452, 604
Bse118I	RCCGGY	1 cut(s)	173
Bse1I	ACTGG	2 cut(s)	274, 666
BseBI	CCWGG	3 cut(s)	378, 384, 651
BseCI	ATCGAT	2 cut(s)	633, 803
BseDI	CCNNGG	2 cut(s)	377, 649
BseGI	GGATG	3 cut(s)	299, 691, 745
BseLI	CCNNNNNNNGG	5 cut(s)	213, 280, 383, 452, 604
BseMII	CTCAG	2 cut(s)	35, 819
BseNI	ACTGG	2 cut(s)	274, 666
BseRI	GAGGAG	3 cut(s)	375, 575, 578
BseSI	GKGCMC	2 cut(s)	102, 438
BseXI	GCAGC	1 cut(s)	688
BshFI	GGCC	4 cut(s)	8, 173, 382, 436
BshNI	GGYRCC	1 cut(s)	386
BshVI	ATCGAT	2 cut(s)	633, 803
BsiHKAI	GWGCWC	1 cut(s)	542
BsiSI	CCGG	1 cut(s)	174
BslI	CCNNNNNNNGG	5 cut(s)	213, 280, 383, 452, 604
BsmAI	GTCTC	1 cut(s)	302
BsnI	GGCC	4 cut(s)	8, 173, 382, 436
Bsp120I	GGGCCC	1 cut(s)	434
Bsp1286I	GDGCHC	3 cut(s)	102, 438, 542
BspACI	CCGC	3 cut(s)	5, 48, 156
BspANI	GGCC	4 cut(s)	8, 173, 382, 436
BspCNI	CTCAG	2 cut(s)	34, 820
BspDI	ATCGAT	2 cut(s)	633, 803
BspLI	GGNNCC	3 cut(s)	178, 388, 436
BspQI	GCTCTTC	1 cut(s)	530
BspT107I	GGYRCC	1 cut(s)	386
BsrBI	CCGCTC	1 cut(s)	50
BsrFI	RCCGGY	1 cut(s)	173
BsrI	ACTGG	2 cut(s)	274, 666
BssAI	RCCGGY	1 cut(s)	173
BssECI	CCNNGG	2 cut(s)	377, 649
BssNI	GRCGYC	1 cut(s)	522
Bst2UI	CCWGG	3 cut(s)	378, 384, 651
Bst4CI	ACNGT	4 cut(s)	13, 421, 623, 644
Bst6I	CTCTTC	2 cut(s)	61, 530
BstACI	GRCGYC	1 cut(s)	522
BstC8I	GCNNGC	2 cut(s)	175, 610
BstDEI	CTNAG	3 cut(s)	21, 326, 828
BstENI	CCTNNNNNAGG	1 cut(s)	381
BstF5I	GGATG	3 cut(s)	299, 691, 745
BstMAI	GTCTC	1 cut(s)	302
BstMWI	GCNNNNNNNGC	2 cut(s)	161, 170
BstNI	CCWGG	3 cut(s)	378, 384, 651
BstSCI	CCNGG	3 cut(s)	376, 382, 649
BstSLI	GKGCMC	2 cut(s)	102, 438
BstV1I	GCAGC	1 cut(s)	688
Bsu15I	ATCGAT	2 cut(s)	633, 803
BsuRI	GGCC	4 cut(s)	8, 173, 382, 436
BsuTUI	ATCGAT	2 cut(s)	633, 803
BtsCI	GGATG	3 cut(s)	299, 691, 745
BtsI	GCAGTG	1 cut(s)	479
BtsIMutI	CAGTG	2 cut(s)	18, 479
Cac8I	GCNNGC	2 cut(s)	175, 610
Cfr10I	RCCGGY	1 cut(s)	173
Cfr13I	GGNCC	3 cut(s)	380, 434, 435
ClaI	ATCGAT	2 cut(s)	633, 803
CseI	GACGC	1 cut(s)	511
Csp6I	GTAC	1 cut(s)	443
CviAII	CATG	3 cut(s)	526, 530, 675
CviQI	GTAC	1 cut(s)	443
DdeI	CTNAG	3 cut(s)	21, 326, 828
DrdI	GACNNNNNNGTC	1 cut(s)	22
DseDI	GACNNNNNNGTC	1 cut(s)	22
EaeI	YGGCCR	2 cut(s)	6, 171
Eam1104I	CTCTTC	2 cut(s)	61, 530
EarI	CTCTTC	2 cut(s)	61, 530
Ecl136II	GAGCTC	1 cut(s)	540
Eco24I	GRGCYC	2 cut(s)	438, 542
Eco32I	GATATC	1 cut(s)	617
Eco53kI	GAGCTC	1 cut(s)	540
Eco57I	CTGAAG	1 cut(s)	789
EcoICRI	GAGCTC	1 cut(s)	540
EcoNI	CCTNNNNNAGG	1 cut(s)	381
EcoO109I	RGGNCCY	1 cut(s)	434
EcoRII	CCWGG	3 cut(s)	376, 382, 649
EcoRV	GATATC	1 cut(s)	617
EcoT22I	ATGCAT	1 cut(s)	531
EcoT38I	GRGCYC	2 cut(s)	438, 542
FaeI	CATG	3 cut(s)	529, 533, 678
FatI	CATG	3 cut(s)	525, 529, 674
FauI	CCCGC	1 cut(s)	55
Fnu4HI	GCNGC	3 cut(s)	6, 156, 702
FokI	GGATG	3 cut(s)	306, 698, 732
FriOI	GRGCYC	2 cut(s)	438, 542
Fsp4HI	GCNGC	3 cut(s)	6, 156, 702
FspBI	CTAG	1 cut(s)	431
GluI	GCNGC	3 cut(s)	6, 156, 702
HaeIII	GGCC	4 cut(s)	8, 173, 382, 436
HapII	CCGG	1 cut(s)	174
HgaI	GACGC	1 cut(s)	511
Hin1I	GRCGYC	1 cut(s)	522
Hin1II	CATG	3 cut(s)	529, 533, 678
HincII	GTYRAC	1 cut(s)	591
HindII	GTYRAC	1 cut(s)	591
HindIII	AAGCTT	1 cut(s)	162
HinfI	GANTC	4 cut(s)	223, 398, 818, 824
HpaI	GTTAAC	1 cut(s)	591
HpaII	CCGG	1 cut(s)	174
HphI	GGTGA	2 cut(s)	296, 665
Hpy166II	GTNNAC	1 cut(s)	591
Hpy188I	TCNGA	5 cut(s)	181, 315, 769, 823, 829
Hpy188III	TCNNGA	5 cut(s)	117, 220, 395, 402, 749
Hpy8I	GTNNAC	1 cut(s)	591
HpyAV	CCTTC	1 cut(s)	764
HpyCH4III	ACNGT	4 cut(s)	13, 421, 623, 644
HpyCH4V	TGCA	6 cut(s)	70, 124, 415, 472, 477, 529
HpyF10VI	GCNNNNNNNGC	2 cut(s)	161, 170
HpyF3I	CTNAG	3 cut(s)	21, 326, 828
Hsp92I	GRCGYC	1 cut(s)	522
Hsp92II	CATG	3 cut(s)	529, 533, 678
KroI	GCCGGC	1 cut(s)	173
KroNI	GCCGGC	1 cut(s)	175
KspAI	GTTAAC	1 cut(s)	591
LguI	GCTCTTC	1 cut(s)	530
LmnI	GCTCC	2 cut(s)	182, 427
Lsp1109I	GCAGC	1 cut(s)	688
LweI	GCATC	2 cut(s)	486, 733
MaeI	CTAG	1 cut(s)	431
MaeIII	GTNAC	3 cut(s)	13, 707, 788
MbiI	CCGCTC	1 cut(s)	50
MboII	GAAGA	5 cut(s)	48, 547, 581, 668, 719
MfeI	CAATTG	1 cut(s)	780
MhlI	GDGCHC	3 cut(s)	102, 438, 542
MluCI	AATT	8 cut(s)	106, 125, 137, 244, 501, 666, 780, 832
MlyI	GAGTC	1 cut(s)	827
MmeI	TCCRAC	1 cut(s)	405
Mph1103I	ATGCAT	1 cut(s)	531
MroNI	GCCGGC	1 cut(s)	173
MseI	TTAA	2 cut(s)	590, 798
MspI	CCGG	1 cut(s)	174
MspR9I	CCNGG	3 cut(s)	378, 384, 651
MunI	CAATTG	1 cut(s)	780
MvaI	CCWGG	3 cut(s)	378, 384, 651
MwoI	GCNNNNNNNGC	2 cut(s)	161, 170
NaeI	GCCGGC	1 cut(s)	175
NgoMIV	GCCGGC	1 cut(s)	173
NlaIII	CATG	3 cut(s)	529, 533, 678
NlaIV	GGNNCC	3 cut(s)	178, 388, 436
NmuCI	GTSAC	2 cut(s)	13, 707
NsiI	ATGCAT	1 cut(s)	531
PciSI	GCTCTTC	1 cut(s)	530
PdiI	GCCGGC	1 cut(s)	175
PfeI	GAWTC	3 cut(s)	223, 398, 824
PflMI	CCANNNNNTGG	1 cut(s)	280
PkrI	GCNGC	3 cut(s)	7, 157, 703
PleI	GAGTC	1 cut(s)	826
PpsI	GAGTC	1 cut(s)	826
PsiI	TTATAA	1 cut(s)	555
Psp124BI	GAGCTC	1 cut(s)	542
Psp6I	CCWGG	3 cut(s)	376, 382, 649
PspGI	CCWGG	3 cut(s)	376, 382, 649
PspN4I	GGNNCC	3 cut(s)	178, 388, 436
PspOMI	GGGCCC	1 cut(s)	434
PspPI	GGNCC	3 cut(s)	380, 434, 435
RsaI	GTAC	1 cut(s)	444
RsaNI	GTAC	1 cut(s)	443
SacI	GAGCTC	1 cut(s)	542
SapI	GCTCTTC	1 cut(s)	530
SaqAI	TTAA	2 cut(s)	590, 798
SatI	GCNGC	3 cut(s)	6, 156, 702
Sau96I	GGNCC	3 cut(s)	380, 434, 435
SchI	GAGTC	1 cut(s)	827
ScrFI	CCNGG	3 cut(s)	378, 384, 651
SduI	GDGCHC	3 cut(s)	102, 438, 542
SetI	ASST	9 cut(s)	20, 89, 166, 257, 263, 432, 542, 578, 655
SfaNI	GCATC	2 cut(s)	486, 733
SmlI	CTYRAG	1 cut(s)	393
SmoI	CTYRAG	1 cut(s)	393
Sse9I	AATT	8 cut(s)	106, 125, 137, 244, 501, 666, 780, 832
SsiI	CCGC	3 cut(s)	5, 48, 156
SspMI	CTAG	1 cut(s)	431
SstI	GAGCTC	1 cut(s)	542
StyD4I	CCNGG	3 cut(s)	376, 382, 649
TaaI	ACNGT	4 cut(s)	13, 421, 623, 644
TaqI	TCGA	3 cut(s)	191, 633, 803
TasI	AATT	8 cut(s)	106, 125, 137, 244, 501, 666, 780, 832
TauI	GCSGC	2 cut(s)	8, 158
TfiI	GAWTC	3 cut(s)	223, 398, 824
Tru1I	TTAA	2 cut(s)	590, 798
Tru9I	TTAA	2 cut(s)	590, 798
TscAI	CASTG	2 cut(s)	18, 479
TseFI	GTSAC	2 cut(s)	13, 707
TseI	GCWGC	1 cut(s)	701
Tsp45I	GTSAC	2 cut(s)	13, 707
TspDTI	ATGAA	2 cut(s)	336, 351
TspRI	CASTG	2 cut(s)	18, 479
Van91I	CCANNNNNTGG	1 cut(s)	280
XagI	CCTNNNNNAGG	1 cut(s)	381
XspI	CTAG	1 cut(s)	431
Zsp2I	ATGCAT	1 cut(s)	531

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.