Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001945

Physical Location & Seq

Genomic Coordinates Reverse (-)

5255797 .. 5260805

Gene Structure 5009 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1065000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1740 bp

ATGACGACCGATCTCAGTGATTATGCCGTCATCAAGGAAGGAGAGGCTGAGATTCTCATGCATGCTAAGAACCAAGTCTTTTACAATAAAACTCAGGTTAACAACAGAGACATATCAGTTGCTGTTCTGAGGACATTCATAACCAAACGCAAGGAGGAGCATGAAGCAAGATTGTCCAAGAAAGCAAAACCTGCACCGAAGGAGTCTGACAAAGATGCTTCAGATTCTGTTGTAGAAGAAGTACCTAATGAGCCTGCTATTTATGAAGAAAAAACCAATGGAGATTGTGAAATGGCAGCAGAGATATCTCAAGATGAACCATGTAGCATTTCAGAAAAAACAGACAAGGTCACTGAGGGAAAATGGCGTGGGGAACTGAAGCCACCAAGAGTTCTTGAGGCCCTGTCAGCTTCTGGTTTAAGAGCTCTTAGGTATGCTCGTGAAGTGGAAGGAATTGGTAAAGTTGTTGCTTTAGACAATGATAAAGGTGCAGTTGAAGCTTGTAGGAGAAATATCAAGTTCAACGGTTCAGTTGCATGTTCAAAGGTGGAATCACATCTTGCTGATGCTCGTGTGCATATGCTCACCCATCCAAAAGAATTTGATGTGGTTGATCTTGATCCTTATGGTTCGCCCTCTGTCTTCTTGGACTCTGCGGTTCAATCTGTTGTTGATGGAGGATTGCTAATGTGTACAGCAACTGATATGGCAGTGCTATGTGGTGGAAATGGGGAGGTTTGCTATTCAAAATATGGTTCCTATCCATTGAGAGGGAAGTATTGTCACGAAATGGCTTTGAGGATCCTCCTAGCTTGCATTGAGAGTCATGCAAATCGTTACAAACGGTACATTGTTCCTGTGCTATCTGTTCAGATGGACTTTTATGTTCGGGTTTTCATTCGTATCTACTCTTCTGCAAGTGCAATGAAGAACTCTCCCCTTAAGCTATCCTATGTTTATCAGTGCATTGGCTGCGATTCATTTCATCTTCAGCCTATTGGAAGGACAGTCACAAAGAACACCAGTGTAAGATATCTACCGGGTTTTGGTCCCGTTGTTCCTCAAGAATGCAGTGACTGTGGGAAGAAATTCAATATGGGTGGACCTATATGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATACTATCTGATGTGAAATCTATGAAGGAGCGGTATCCCGCTTATGATCGCATCTCTGCTGTACTAACAACAATTTCAGAGGAACTGCCTGATGTTCCTTTGTTCTTGAGTCTGCACAACCTCTGTGCCACATTAAAGTGCACTTCCCCATCTGCTGTGATTTTCCGTTCTGCTGTGATCAATGCAGGGTATCGTATATCTGGAACTCACGTAAACCCATTGGGGTTGAAATCAGATGCTCCCATGGATGTCATTTGGGACATAATGCGCTGTTGGGTGAAGAATCACCCCGTGAAACCTCAGCCAGCGGATCAGTCAGGGACCGTGATACTTGCCAAGGAACCCATCCTTCAAGCTAACTTTGCTCGAGCTGTTGCGTCTCTTAGCAAGGCACAAACCAAGAAGGTAGCACGCTTCCTTCCAAATCCTGAAAGGCACTGGGGGCCAAAGCTTAGGGCAGGTCGTACAATCACCAGCAAACACATCTCTCTCTTGGGTCCGGAGGCTGTCAATGGACATCTTAACCATGAAGATGGTGAGGAACATGATGCCAAGCGTCAAAAGATTGAAGATCAACCCACCCCAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0002940 GO:0003674 GO:0003824 GO:0004809 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008175 GO:0008757 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016423 GO:0016740 GO:0016741 GO:0030488 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TRM

Protein Analysis

580

Amino Acids

64.13

Weight (kDa)

7.82

Isoelectric Point (pI)

43.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TRM	PF02005	11 - 473	1.2e-117	N2,N2-dimethylguanosine tRNA methyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02320 AT5G15810
fragaria_vesca	FvH4_6g46450 FvH4_6g46450 FvH4_6g46450
malus_domestica	MD09G1073500.v1.1 MD17G1065000.v1.1
prunus_persica	Prupe.3G249800_v2.0.a1 Prupe.3G249800_v2.0.a1
pyrus_communis	pycom09g00090 pycom17g06500
rosa_chinensis	RchiOBHm_Chr2g0130081 RchiOBHm_Chr2g0165001
rosa_laevigata	RLG00000008503 RLG00000021506
rosa_multiflora	Rmu_co8255059.1_g000001 Rmu_sc0001097.1_g000019 Rmu_sc0002970.1_g000011 Rmu_sc0022378.1_g000001 Rmu_sc0026009.1_g000001
rosa_roxburghii	Rroxscaffold_1G00025420 Rroxscaffold_1G00038210 Rroxscaffold_1G00074000 Rroxscaffold_2G00083080 Rroxscaffold_5G00359260 Rroxscaffold_7G00169970 Rroxscaffold_7G00192270 Rroxscaffold_7G00205690
rosa_rugosa	Rorug02G0291000 Rorug02G0512100
rosa_samantha	Rh1AG215300 Rh1CG199800 Rh1DG211500 Rh2AG509800 Rh2AG580200 Rh2BG350400 Rh2BG572900 Rh2BG591500 Rh2CG495000 Rh2CG562000 Rh2DG601600 Rh4CG060300
rosa_wichuraiana	Rw0G021070 Rw2G027680 Rw2G048160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	199, 1598
AccBSI	CCGCTC	1 cut(s)	1180
AccIII	TCCGGA	1 cut(s)	1648
AciI	CCGC	4 cut(s)	656, 1180, 1188, 1457
AclWI	GGATC	4 cut(s)	614, 796, 809, 1467
AcsI	RAATTY	2 cut(s)	599, 1088
AcuI	CTGAAG	3 cut(s)	204, 398, 974
AdeI	CACNNNGTG	1 cut(s)	1441
AfaI	GTAC	5 cut(s)	243, 694, 848, 1212, 1615
AfiI	CCNNNNNNNGG	2 cut(s)	770, 1046
AflII	CTTAAG	1 cut(s)	941
AgsI	TTSAA	9 cut(s)	497, 523, 543, 662, 747, 1093, 1378, 1502, 1718
AluBI	AGCT	8 cut(s)	410, 425, 500, 812, 946, 1505, 1520, 1600
AluI	AGCT	8 cut(s)	410, 425, 500, 812, 946, 1505, 1520, 1600
Alw21I	GWGCWC	2 cut(s)	427, 1292
Alw26I	GTCTC	2 cut(s)	102, 1533
Alw44I	GTGCAC	1 cut(s)	1288
AlwI	GGATC	4 cut(s)	614, 796, 809, 1467
AlwNI	CAGNNNCTG	5 cut(s)	122, 227, 413, 701, 1077
Ama87I	CYCGRG	1 cut(s)	1515
Aor13HI	TCCGGA	1 cut(s)	1648
AoxI	GGCC	2 cut(s)	399, 1592
ApaLI	GTGCAC	1 cut(s)	1288
ApeKI	GCWGC	2 cut(s)	296, 972
ApoI	RAATTY	2 cut(s)	599, 1088
Asp700I	GAANNNNTTC	1 cut(s)	1088
AspLEI	GCGC	1 cut(s)	1419
AspS9I	GGNCC	6 cut(s)	400, 1049, 1103, 1470, 1592, 1646
AsuC2I	CCSGG	1 cut(s)	1041
AsuHPI	GGTGA	5 cut(s)	577, 1427, 1438, 1612, 1697
AvaI	CYCGRG	1 cut(s)	1515
AvaII	GGWCC	4 cut(s)	1049, 1103, 1470, 1646
BaeGI	GKGCMC	1 cut(s)	1292
BamHI	GGATCC	1 cut(s)	801
BanII	GRGCYC	1 cut(s)	427
BarI	GAAGNNNNNNTAC	2 cut(s)	895, 927
BauI	CACGAG	2 cut(s)	438, 570
BbsI	GAAGAC	1 cut(s)	634
Bbv12I	GWGCWC	2 cut(s)	427, 1292
BbvCI	CCTCAGC	1 cut(s)	1449
BbvI	GCAGC	2 cut(s)	308, 959
BccI	CCATC	6 cut(s)	597, 668, 868, 1306, 1502, 1676
BceAI	ACGGC	1 cut(s)	11
BciVI	GTATCC	1 cut(s)	1194
BclI	TGATCA	2 cut(s)	1129, 1326
BcnI	CCSGG	1 cut(s)	1041
BcoDI	GTCTC	2 cut(s)	102, 1533
BfaI	CTAG	1 cut(s)	809
BfrI	CTTAAG	1 cut(s)	941
BfuAI	ACCTGC	2 cut(s)	199, 1598
BfuI	GTATCC	1 cut(s)	1194
BisI	GCNGC	2 cut(s)	297, 973
BlsI	GCNGC	2 cut(s)	298, 974
Bme1390I	CCNGG	1 cut(s)	1041
Bme18I	GGWCC	4 cut(s)	1049, 1103, 1470, 1646
BmeT110I	CYCGRG	1 cut(s)	1515
BmgT120I	GGNCC	6 cut(s)	400, 1049, 1103, 1470, 1592, 1646
BmiI	GGNNCC	7 cut(s)	757, 803, 1051, 1471, 1491, 1593, 1647
BmrFI	CCNGG	1 cut(s)	1041
BmrI	ACTGGG	1 cut(s)	1597
BmsI	GCATC	5 cut(s)	205, 556, 1209, 1375, 1687
BmuI	ACTGGG	1 cut(s)	1597
BpiI	GAAGAC	1 cut(s)	634
Bpu10I	CCTNAGC	2 cut(s)	1449, 1601
BpuEI	CTTGAG	4 cut(s)	294, 416, 1047, 1276
BpuMI	CCSGG	1 cut(s)	1041
BsaAI	YACGTR	1 cut(s)	1360
BsaBI	GATNNNNATC	1 cut(s)	618
BsaJI	CCNNGG	2 cut(s)	1392, 1485
BsaWI	WCCGGW	1 cut(s)	1648
Bsc4I	CCNNNNNNNGG	2 cut(s)	770, 1046
Bse1I	ACTGG	2 cut(s)	1023, 1592
Bse3DI	GCAATG	1 cut(s)	930
Bse8I	GATNNNNATC	1 cut(s)	618
BseAI	TCCGGA	1 cut(s)	1648
BseDI	CCNNGG	2 cut(s)	1392, 1485
BseGI	GGATG	3 cut(s)	589, 1402, 1494
BseJI	GATNNNNATC	1 cut(s)	618
BseLI	CCNNNNNNNGG	2 cut(s)	770, 1046
BseMI	GCAATG	1 cut(s)	930
BseMII	CTCAG	6 cut(s)	28, 39, 107, 119, 345, 1463
BseNI	ACTGG	2 cut(s)	1023, 1592
BseRI	GAGGAG	1 cut(s)	170
BseSI	GKGCMC	1 cut(s)	1292
BseXI	GCAGC	2 cut(s)	308, 959
BsgI	GTGCAG	3 cut(s)	177, 510, 1247
Bsh1285I	CGRYCG	1 cut(s)	9
BshFI	GGCC	2 cut(s)	401, 1594
BsiEI	CGRYCG	1 cut(s)	9
BsiHKAI	GWGCWC	2 cut(s)	427, 1292
BsiHKCI	CYCGRG	1 cut(s)	1515
BsiSI	CCGG	2 cut(s)	1040, 1649
BslFI	GGGAC	3 cut(s)	1035, 1421, 1483
BslI	CCNNNNNNNGG	2 cut(s)	770, 1046
BsmAI	GTCTC	2 cut(s)	102, 1533
BsmBI	CGTCTC	1 cut(s)	1533
BsmFI	GGGAC	3 cut(s)	1035, 1421, 1483
BsmI	GAATGC	1 cut(s)	1073
BsnI	GGCC	2 cut(s)	401, 1594
BsoBI	CYCGRG	1 cut(s)	1515
Bsp1286I	GDGCHC	2 cut(s)	427, 1292
Bsp13I	TCCGGA	1 cut(s)	1648
Bsp1407I	TGTACA	1 cut(s)	692
Bsp143I	GATC	9 cut(s)	10, 613, 619, 801, 1129, 1195, 1326, 1459, 1720
Bsp19I	CCATGG	1 cut(s)	1392
BspACI	CCGC	4 cut(s)	656, 1180, 1188, 1457
BspANI	GGCC	2 cut(s)	401, 1594
BspCNI	CTCAG	6 cut(s)	27, 40, 106, 120, 346, 1462
BspEI	TCCGGA	1 cut(s)	1648
BspLI	GGNNCC	7 cut(s)	757, 803, 1051, 1471, 1491, 1593, 1647
BspMI	ACCTGC	2 cut(s)	199, 1598
BspPI	GGATC	4 cut(s)	614, 796, 809, 1467
BspTI	CTTAAG	1 cut(s)	941
BsrBI	CCGCTC	1 cut(s)	1180
BsrDI	GCAATG	1 cut(s)	930
BsrGI	TGTACA	1 cut(s)	692
BsrI	ACTGG	2 cut(s)	1023, 1592
BssECI	CCNNGG	2 cut(s)	1392, 1485
BssMI	GATC	9 cut(s)	10, 613, 619, 801, 1129, 1195, 1326, 1459, 1720
BssSI	CACGAG	2 cut(s)	438, 570
BssT1I	CCWWGG	2 cut(s)	1392, 1485
Bst2BI	CACGAG	2 cut(s)	438, 570
Bst4CI	ACNGT	5 cut(s)	527, 846, 1009, 1079, 1474
Bst6I	CTCTTC	1 cut(s)	916
BstAFI	CTTAAG	1 cut(s)	941
BstAPI	GCANNNNNTGC	2 cut(s)	191, 972
BstAUI	TGTACA	1 cut(s)	692
BstBAI	YACGTR	1 cut(s)	1360
BstC8I	GCNNGC	5 cut(s)	63, 255, 814, 1455, 1561
BstDSI	CCRYGG	1 cut(s)	1392
BstF5I	GGATG	3 cut(s)	589, 1402, 1494
BstHHI	GCGC	1 cut(s)	1419
BstKTI	GATC	9 cut(s)	13, 616, 622, 804, 1132, 1198, 1329, 1462, 1723
BstMAI	GTCTC	2 cut(s)	102, 1533
BstMBI	GATC	9 cut(s)	10, 613, 619, 801, 1129, 1195, 1326, 1459, 1720
BstMCI	CGRYCG	1 cut(s)	9
BstMWI	GCNNNNNNNGC	6 cut(s)	191, 407, 497, 972, 1511, 1591
BstNSI	RCATGY	2 cut(s)	65, 540
BstSCI	CCNGG	1 cut(s)	1039
BstSLI	GKGCMC	1 cut(s)	1292
BstV1I	GCAGC	2 cut(s)	308, 959
BstV2I	GAAGAC	1 cut(s)	634
BstX2I	RGATCY	1 cut(s)	801
BstXI	CCANNNNNNTGG	1 cut(s)	1682
BstYI	RGATCY	1 cut(s)	801
BsuI	GTATCC	1 cut(s)	1194
BsuRI	GGCC	2 cut(s)	401, 1594
BtgI	CCRYGG	1 cut(s)	1392
BtsCI	GGATG	3 cut(s)	589, 1402, 1494
BtsI	GCAGTG	2 cut(s)	717, 1078
BtsIMutI	CAGTG	7 cut(s)	22, 351, 717, 968, 1030, 1078, 1585
BveI	ACCTGC	2 cut(s)	199, 1598
Cac8I	GCNNGC	5 cut(s)	63, 255, 814, 1455, 1561
CaiI	CAGNNNCTG	5 cut(s)	122, 227, 413, 701, 1077
CfoI	GCGC	1 cut(s)	1419
Cfr13I	GGNCC	6 cut(s)	400, 1049, 1103, 1470, 1592, 1646
CseI	GACGC	2 cut(s)	1515, 1694
Csp6I	GTAC	5 cut(s)	242, 693, 847, 1211, 1614
CspCI	CAANNNNNGTGG	2 cut(s)	1081, 1116
CviQI	GTAC	5 cut(s)	242, 693, 847, 1211, 1614
DpnI	GATC	9 cut(s)	12, 615, 621, 803, 1131, 1197, 1328, 1461, 1722
DpnII	GATC	9 cut(s)	10, 613, 619, 801, 1129, 1195, 1326, 1459, 1720
DraIII	CACNNNGTG	1 cut(s)	1441
Eam1104I	CTCTTC	1 cut(s)	916
EarI	CTCTTC	1 cut(s)	916
Ecl136II	GAGCTC	1 cut(s)	425
Eco130I	CCWWGG	2 cut(s)	1392, 1485
Eco24I	GRGCYC	1 cut(s)	427
Eco32I	GATATC	2 cut(s)	306, 1034
Eco47I	GGWCC	4 cut(s)	1049, 1103, 1470, 1646
Eco53kI	GAGCTC	1 cut(s)	425
Eco57I	CTGAAG	3 cut(s)	204, 398, 974
Eco88I	CYCGRG	1 cut(s)	1515
EcoICRI	GAGCTC	1 cut(s)	425
EcoO109I	RGGNCCY	1 cut(s)	400
EcoRV	GATATC	2 cut(s)	306, 1034
EcoT14I	CCWWGG	2 cut(s)	1392, 1485
EcoT22I	ATGCAT	1 cut(s)	63
EcoT38I	GRGCYC	1 cut(s)	427
ErhI	CCWWGG	2 cut(s)	1392, 1485
Esp3I	CGTCTC	1 cut(s)	1533
FaqI	GGGAC	3 cut(s)	1035, 1421, 1483
FauI	CCCGC	1 cut(s)	1195
FauNDI	CATATG	1 cut(s)	579
FbaI	TGATCA	2 cut(s)	1129, 1326
Fnu4HI	GCNGC	2 cut(s)	297, 973
FokI	GGATG	3 cut(s)	576, 1409, 1481
FriOI	GRGCYC	1 cut(s)	427
Fsp4HI	GCNGC	2 cut(s)	297, 973
FspBI	CTAG	1 cut(s)	809
GlaI	GCGC	1 cut(s)	1418
GluI	GCNGC	2 cut(s)	297, 973
HaeIII	GGCC	2 cut(s)	401, 1594
HapII	CCGG	2 cut(s)	1040, 1649
HgaI	GACGC	2 cut(s)	1515, 1694
HhaI	GCGC	1 cut(s)	1419
Hin6I	GCGC	1 cut(s)	1417
HinP1I	GCGC	1 cut(s)	1417
HincII	GTYRAC	1 cut(s)	100
HindII	GTYRAC	1 cut(s)	100
HindIII	AAGCTT	2 cut(s)	498, 1598
HinfI	GANTC	9 cut(s)	52, 203, 224, 551, 650, 823, 977, 1258, 1432
HpaI	GTTAAC	1 cut(s)	100
HpaII	CCGG	2 cut(s)	1040, 1649
HphI	GGTGA	5 cut(s)	577, 1427, 1438, 1612, 1697
Hpy166II	GTNNAC	5 cut(s)	100, 693, 1103, 1290, 1363
Hpy188I	TCNGA	8 cut(s)	129, 208, 223, 334, 873, 1159, 1228, 1384
Hpy8I	GTNNAC	5 cut(s)	100, 693, 1103, 1290, 1363
HpyAV	CCTTC	8 cut(s)	32, 193, 443, 996, 1168, 1508, 1546, 1577
HpyCH4III	ACNGT	5 cut(s)	527, 846, 1009, 1079, 1474
HpyCH4IV	ACGT	1 cut(s)	1359
HpyF10VI	GCNNNNNNNGC	6 cut(s)	191, 407, 497, 972, 1511, 1591
HpySE526I	ACGT	1 cut(s)	1359
HspAI	GCGC	1 cut(s)	1417
Kpn2I	TCCGGA	1 cut(s)	1648
Ksp22I	TGATCA	2 cut(s)	1129, 1326
KspAI	GTTAAC	1 cut(s)	100
Kzo9I	GATC	9 cut(s)	10, 613, 619, 801, 1129, 1195, 1326, 1459, 1720
LmnI	GCTCC	4 cut(s)	157, 1123, 1177, 1393
Lsp1109I	GCAGC	2 cut(s)	308, 959
LweI	GCATC	5 cut(s)	205, 556, 1209, 1375, 1687
MaeI	CTAG	1 cut(s)	809
MaeII	ACGT	1 cut(s)	1359
MaeIII	GTNAC	6 cut(s)	349, 782, 836, 1009, 1073, 1141
MalI	GATC	9 cut(s)	12, 615, 621, 803, 1131, 1197, 1328, 1461, 1722
MbiI	CCGCTC	1 cut(s)	1180
MboI	GATC	9 cut(s)	10, 613, 619, 801, 1129, 1195, 1326, 1459, 1720
MflI	RGATCY	1 cut(s)	801
MhlI	GDGCHC	2 cut(s)	427, 1292
MluCI	AATT	5 cut(s)	453, 599, 1088, 1221, 1735
MlyI	GAGTC	4 cut(s)	212, 644, 832, 1267
Mph1103I	ATGCAT	1 cut(s)	63
MroI	TCCGGA	1 cut(s)	1648
MroXI	GAANNNNTTC	1 cut(s)	1088
MseI	TTAA	5 cut(s)	99, 419, 942, 1283, 1671
MslI	CAYNNNNRTG	2 cut(s)	1285, 1680
MspA1I	CMGCKG	1 cut(s)	1457
MspCI	CTTAAG	1 cut(s)	941
MspI	CCGG	2 cut(s)	1040, 1649
MspR9I	CCNGG	1 cut(s)	1041
Mva1269I	GAATGC	1 cut(s)	1073
MwoI	GCNNNNNNNGC	6 cut(s)	191, 407, 497, 972, 1511, 1591
NciI	CCSGG	1 cut(s)	1041
NcoI	CCATGG	1 cut(s)	1392
NdeI	CATATG	1 cut(s)	579
NdeII	GATC	9 cut(s)	10, 613, 619, 801, 1129, 1195, 1326, 1459, 1720
NlaIV	GGNNCC	7 cut(s)	757, 803, 1051, 1471, 1491, 1593, 1647
NmuCI	GTSAC	4 cut(s)	349, 782, 1009, 1073
NsiI	ATGCAT	1 cut(s)	63
NspI	RCATGY	2 cut(s)	65, 540
PaeI	GCATGC	1 cut(s)	65
PaeR7I	CTCGAG	1 cut(s)	1515
PctI	GAATGC	1 cut(s)	1073
PdmI	GAANNNNTTC	1 cut(s)	1088
PfeI	GAWTC	5 cut(s)	52, 224, 551, 977, 1432
PflFI	GACNNNGTC	1 cut(s)	347
PkrI	GCNGC	2 cut(s)	298, 974
PleI	GAGTC	4 cut(s)	211, 644, 831, 1266
PpsI	GAGTC	4 cut(s)	211, 644, 831, 1266
Ppu21I	YACGTR	1 cut(s)	1360
Psp124BI	GAGCTC	1 cut(s)	427
PspN4I	GGNNCC	7 cut(s)	757, 803, 1051, 1471, 1491, 1593, 1647
PspPI	GGNCC	6 cut(s)	400, 1049, 1103, 1470, 1592, 1646
PspXI	VCTCGAGB	1 cut(s)	1515
PstNI	CAGNNNCTG	5 cut(s)	122, 227, 413, 701, 1077
PsuI	RGATCY	1 cut(s)	801
PsyI	GACNNNGTC	1 cut(s)	347
RsaI	GTAC	5 cut(s)	243, 694, 848, 1212, 1615
RsaNI	GTAC	5 cut(s)	242, 693, 847, 1211, 1614
RseI	CAYNNNNRTG	2 cut(s)	1285, 1680
SacI	GAGCTC	1 cut(s)	427
SaqAI	TTAA	5 cut(s)	99, 419, 942, 1283, 1671
SatI	GCNGC	2 cut(s)	297, 973
Sau3AI	GATC	9 cut(s)	10, 613, 619, 801, 1129, 1195, 1326, 1459, 1720
Sau96I	GGNCC	6 cut(s)	400, 1049, 1103, 1470, 1592, 1646
SchI	GAGTC	4 cut(s)	212, 644, 832, 1267
ScrFI	CCNGG	1 cut(s)	1041
SduI	GDGCHC	2 cut(s)	427, 1292
SfaNI	GCATC	5 cut(s)	205, 556, 1209, 1375, 1687
Sfr274I	CTCGAG	1 cut(s)	1515
SinI	GGWCC	4 cut(s)	1049, 1103, 1470, 1646
SlaI	CTCGAG	1 cut(s)	1515
SmiMI	CAYNNNNRTG	2 cut(s)	1285, 1680
SmlI	CTYRAG	6 cut(s)	309, 395, 941, 1062, 1255, 1515
SmoI	CTYRAG	6 cut(s)	309, 395, 941, 1062, 1255, 1515
SphI	GCATGC	1 cut(s)	65
Sse9I	AATT	5 cut(s)	453, 599, 1088, 1221, 1735
SsiI	CCGC	4 cut(s)	656, 1180, 1188, 1457
SspMI	CTAG	1 cut(s)	809
SstI	GAGCTC	1 cut(s)	427
StyD4I	CCNGG	1 cut(s)	1039
StyI	CCWWGG	2 cut(s)	1392, 1485
TaaI	ACNGT	5 cut(s)	527, 846, 1009, 1079, 1474
TaiI	ACGT	1 cut(s)	1362
TaqI	TCGA	1 cut(s)	1516
TaqII	GACCGA	1 cut(s)	23
TasI	AATT	5 cut(s)	453, 599, 1088, 1221, 1735
TatI	WGTACW	2 cut(s)	692, 1210
TfiI	GAWTC	5 cut(s)	52, 224, 551, 977, 1432
Tru1I	TTAA	5 cut(s)	99, 419, 942, 1283, 1671
Tru9I	TTAA	5 cut(s)	99, 419, 942, 1283, 1671
TscAI	CASTG	7 cut(s)	22, 358, 717, 968, 1030, 1078, 1592
TseFI	GTSAC	4 cut(s)	349, 782, 1009, 1073
TseI	GCWGC	2 cut(s)	296, 972
Tsp45I	GTSAC	4 cut(s)	349, 782, 1009, 1073
TspGWI	ACGGA	1 cut(s)	1304
TspRI	CASTG	7 cut(s)	22, 358, 717, 968, 1030, 1078, 1592
Tth111I	GACNNNGTC	1 cut(s)	347
Vha464I	CTTAAG	1 cut(s)	941
VneI	GTGCAC	1 cut(s)	1288
VpaK11BI	GGWCC	4 cut(s)	1049, 1103, 1470, 1646
XapI	RAATTY	2 cut(s)	599, 1088
XceI	RCATGY	2 cut(s)	65, 540
XhoI	CTCGAG	1 cut(s)	1515
XmnI	GAANNNNTTC	1 cut(s)	1088
XspI	CTAG	1 cut(s)	809
Zsp2I	ATGCAT	1 cut(s)	63

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.