RchiOBHm_Chr2g0165001

tRNA (Guanine(26)-N(2))-dimethyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001945
Physical Location & Seq
Forward (+)
80039575 .. 80044708
5134 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53298

Sequence Viewer

Length: 1740 bp
ATGTCGACGGATCTCAGTGATTTCATCGTCATCAAAGAAGGAGAGGCTGAGATTCTCATGCATACTAAAAACCAAGTCTTTTACAACAAAACCCAGGTTAACAATAGAGACATCTCGATTGCTGTTATGAGGGCATTTGTTGCCAAACGCAAAGAGGAGCATGAAGCGAGATTGTCCAAGAAAAAAAAGACAGCTCCCACGGATTCTGAGAAAGATGTTTCAGAATCTGTTGTAGAAGAAGCATCTAATGAACCTGCTATTAATGGAAATTCCAATGGAGATTGTGAAATGCCAGAAGATATATCTCAAGATGAACCATGTAGCATTCCAGAAGAACCAAGCAAGACCACAGAGGGAAAAGGACGAGGGGAATTGAAGCCACCAAGGGTTCTTGAGGCCTTGTCAGCTTCTGGCTTAAGAGCTCTTAGGTATGCTCGTGAAGTAGAAGGGATTGGTCAAGTTGTGGCCCTAGATAATGATAAAGTATCAGTTGAAGCTTGTAGGAGAAACATCAAGTTCAATGGTTCAGTTGCATGTTCAAAGGTGGAATCACATCTTGTTGATGCTCGTGTGCATATGCTCACCCACCCAAAAGAATTCGATGTGGTTGATCTTGATCCTTATGGTTCTCCTTCTGTCTTCTTGGATTCTGCGATTCAAGCTGTTGCTGATGGAGGCATGCTCATGTGTACCGCAACGGATATGGCAGTACTATGCGGTGGTAATGGGGAGGTTTGCTATTCAAAATATGGTTCCTATCCATTGAAAGGGAAATATTGCCACGAAATGGCTCTGAGGATCCTCTTAGCCTGCATTGAGAGCCATGCAAATCGGTACAAAAGATACATTGTTCCTGTGCTATCTGTTCAGATGGACTTTTATGTTCGTGTTTTCGTCCGAGTCTACTCCTCGGCAAGTGCTATGAAGAACACTCCCCTTAAGCTTTCCTATGTTTATCAGTGCATTGGATGTGATTCTTTTCATCTGCAGCCTCTTGGAAGGACGGTCACTAAGAACACCAGTGTGAGATATCTTCCGGGTTTTGGTCCCATTGTTCCTCAAGAATGCAGTGATTGTGGGAAGAAATTCAATATGGGTGGGCCTATATGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATACTATCAGATGTGAAATCAATGAAGGATCGTTATCCCGCTTATGATCGCATCTCGGCTGTATTGACAACAATCTCAGAGGAATTGCCAGATGTTCCTCTGTTCTTGAGTCTGCACAGCCTCTGTGCCACTCTCAAGTGCACTTCCCCATCCGCAGTGATTTTCCGTTCTGCTGTGATCAATGCAGGATATCGTATATCTGGAACTCATGTAAACCCATTGGGGTTGAAGTCAGATGCTCCTATGGATATCATTTGGGACATAATGCGCTGCTGGGTGAAAAATCATCCAGTGAAAGCTCAACCTCCAGAACATTCAGGAAGCGTGATACTTGCTAAGGAACCTGTTCTTCAAGCAAATTTTGCTCGAGCTGTAGCGTCTCTTAGCAAGGCACAGACCAAGAAAGTTGCACGGTTCCTTCCAAACCCAGAAAGGCATTGGGGTCCAAAGCTTAGGGCAGGTCGTCAAATCACCAGCAAGCACATTTCTCTTTTGGGTCCAGAGGCTGTCAATGAAATTCTTAACCAGGAAGAAGATGGTGAGGAGCACAATGCCAAGCGTCAAAAGACTGAAGATAATCCGCCCTCAGGTTGA

Protein Analysis

579

Amino Acids

63.92

Weight (kDa)

6.96

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRM PF02005 12 - 472 4.2e-115 N2,N2-dimethylguanosine tRNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 262, 1595
AccB7I CCANNNNNTGG 1 cut(s) 787
AccI GTMKAC 2 cut(s) 5, 903
AciI CCGC 5 cut(s) 693, 717, 1185, 1299, 1727
AclWI GGATC 5 cut(s) 18, 611, 793, 806, 1182
AcsI RAATTY 5 cut(s) 268, 596, 1085, 1504, 1662
AcuI CTGAAG 1 cut(s) 1737
AfaI GTAC 3 cut(s) 691, 711, 836
AfiI CCNNNNNNNGG 4 cut(s) 767, 787, 1043, 1733
AflII CTTAAG 2 cut(s) 415, 938
AjnI CCWGG 2 cut(s) 93, 1671
AleI CACNNNNGTG 1 cut(s) 1022
AluBI AGCT 9 cut(s) 194, 407, 422, 497, 662, 943, 1445, 1517, 1597
AluI AGCT 9 cut(s) 194, 407, 422, 497, 662, 943, 1445, 1517, 1597
Alw21I GWGCWC 3 cut(s) 424, 1289, 1695
Alw26I GTCTC 2 cut(s) 102, 1530
Alw44I GTGCAC 1 cut(s) 1285
AlwI GGATC 5 cut(s) 18, 611, 793, 806, 1182
AlwNI CAGNNNCTG 4 cut(s) 227, 410, 1269, 1652
Ama87I CYCGRG 1 cut(s) 1512
AoxI GGCC 3 cut(s) 396, 465, 1100
ApaLI GTGCAC 1 cut(s) 1285
ApeKI GCWGC 2 cut(s) 988, 1416
ApoI RAATTY 5 cut(s) 268, 596, 1085, 1504, 1662
AseI ATTAAT 1 cut(s) 261
Asp700I GAANNNNTTC 2 cut(s) 1085, 1491
AspLEI GCGC 1 cut(s) 1416
AspS9I GGNCC 5 cut(s) 466, 1046, 1100, 1589, 1643
AsuC2I CCSGG 1 cut(s) 1038
AsuHPI GGTGA 4 cut(s) 574, 1435, 1609, 1697
AvaI CYCGRG 1 cut(s) 1512
AvaII GGWCC 3 cut(s) 1046, 1589, 1643
AxyI CCTNAGG 1 cut(s) 1732
BaeGI GKGCMC 1 cut(s) 1289
BamHI GGATCC 1 cut(s) 798
BanII GRGCYC 1 cut(s) 424
BauI CACGAG 2 cut(s) 435, 567
BbsI GAAGAC 1 cut(s) 631
Bbv12I GWGCWC 3 cut(s) 424, 1289, 1695
BbvI GCAGC 2 cut(s) 1000, 1403
BccI CCATC 4 cut(s) 665, 865, 1303, 1676
BciT130I CCWGG 2 cut(s) 95, 1673
BclI TGATCA 2 cut(s) 1126, 1323
BcnI CCSGG 1 cut(s) 1038
BcoDI GTCTC 2 cut(s) 102, 1530
BfaI CTAG 1 cut(s) 470
BfmI CTRYAG 2 cut(s) 986, 1518
BfrI CTTAAG 2 cut(s) 415, 938
BfuAI ACCTGC 2 cut(s) 262, 1595
BisI GCNGC 2 cut(s) 989, 1417
BlsI GCNGC 2 cut(s) 990, 1418
BmcAI AGTACT 1 cut(s) 711
Bme1390I CCNGG 3 cut(s) 95, 1038, 1673
Bme18I GGWCC 3 cut(s) 1046, 1589, 1643
BmeT110I CYCGRG 1 cut(s) 1512
BmgT120I GGNCC 5 cut(s) 466, 1046, 1100, 1589, 1643
BmiI GGNNCC 7 cut(s) 754, 800, 1048, 1488, 1562, 1590, 1644
BmrFI CCNGG 3 cut(s) 95, 1038, 1673
BmsI GCATC 4 cut(s) 251, 553, 1206, 1372
BpiI GAAGAC 1 cut(s) 631
BplI GAGNNNNNCTC 2 cut(s) 666, 698
BpmI CTGGAG 1 cut(s) 1437
Bpu10I CCTNAGC 2 cut(s) 1482, 1598
BpuEI CTTGAG 5 cut(s) 291, 413, 1044, 1265, 1273
BpuMI CCSGG 1 cut(s) 1038
BsaBI GATNNNNATC 2 cut(s) 615, 1179
BsaJI CCNNGG 4 cut(s) 93, 198, 383, 909
Bsc4I CCNNNNNNNGG 4 cut(s) 767, 787, 1043, 1733
Bse1I ACTGG 2 cut(s) 1020, 1436
Bse21I CCTNAGG 1 cut(s) 1732
Bse8I GATNNNNATC 2 cut(s) 615, 1179
BseBI CCWGG 2 cut(s) 95, 1673
BseDI CCNNGG 4 cut(s) 93, 198, 383, 909
BseGI GGATG 3 cut(s) 974, 1295, 1432
BseJI GATNNNNATC 2 cut(s) 615, 1179
BseLI CCNNNNNNNGG 4 cut(s) 767, 787, 1043, 1733
BseMII CTCAG 5 cut(s) 28, 39, 198, 785, 1236
BseNI ACTGG 2 cut(s) 1020, 1436
BseRI GAGGAG 3 cut(s) 170, 898, 1703
BseSI GKGCMC 1 cut(s) 1289
BseXI GCAGC 2 cut(s) 1000, 1403
BseYI CCCAGC 1 cut(s) 1419
BsgI GTGCAG 1 cut(s) 1244
BshFI GGCC 3 cut(s) 398, 467, 1102
BsiHKAI GWGCWC 3 cut(s) 424, 1289, 1695
BsiHKCI CYCGRG 1 cut(s) 1512
BsiSI CCGG 1 cut(s) 1037
BslFI GGGAC 2 cut(s) 1032, 1418
BslI CCNNNNNNNGG 4 cut(s) 767, 787, 1043, 1733
BsmAI GTCTC 2 cut(s) 102, 1530
BsmBI CGTCTC 1 cut(s) 1530
BsmFI GGGAC 2 cut(s) 1032, 1418
BsmI GAATGC 2 cut(s) 324, 1070
BsnI GGCC 3 cut(s) 398, 467, 1102
BsoBI CYCGRG 1 cut(s) 1512
Bsp1286I GDGCHC 3 cut(s) 424, 1289, 1695
Bsp143I GATC 8 cut(s) 10, 610, 616, 798, 1126, 1174, 1192, 1323
BspACI CCGC 5 cut(s) 693, 717, 1185, 1299, 1727
BspANI GGCC 3 cut(s) 398, 467, 1102
BspCNI CTCAG 5 cut(s) 27, 40, 199, 786, 1235
BspLI GGNNCC 7 cut(s) 754, 800, 1048, 1488, 1562, 1590, 1644
BspMAI CTGCAG 1 cut(s) 990
BspMI ACCTGC 2 cut(s) 262, 1595
BspPI GGATC 5 cut(s) 18, 611, 793, 806, 1182
BspTI CTTAAG 2 cut(s) 415, 938
BsrI ACTGG 2 cut(s) 1020, 1436
BssECI CCNNGG 4 cut(s) 93, 198, 383, 909
BssMI GATC 8 cut(s) 10, 610, 616, 798, 1126, 1174, 1192, 1323
BssSI CACGAG 2 cut(s) 435, 567
BssT1I CCWWGG 1 cut(s) 383
Bst2BI CACGAG 2 cut(s) 435, 567
Bst2UI CCWGG 2 cut(s) 95, 1673
Bst4CI ACNGT 2 cut(s) 1006, 1560
BstAFI CTTAAG 2 cut(s) 415, 938
BstAPI GCANNNNNTGC 2 cut(s) 140, 1508
BstC8I GCNNGC 3 cut(s) 680, 811, 1625
BstDSI CCRYGG 1 cut(s) 198
BstF5I GGATG 3 cut(s) 974, 1295, 1432
BstHHI GCGC 1 cut(s) 1416
BstKTI GATC 8 cut(s) 13, 613, 619, 801, 1129, 1177, 1195, 1326
BstMAI GTCTC 2 cut(s) 102, 1530
BstMBI GATC 8 cut(s) 10, 610, 616, 798, 1126, 1174, 1192, 1323
BstMWI GCNNNNNNNGC 5 cut(s) 140, 404, 659, 819, 1508
BstNI CCWGG 2 cut(s) 95, 1673
BstNSI RCATGY 2 cut(s) 537, 682
BstSCI CCNGG 3 cut(s) 93, 1036, 1671
BstSFI CTRYAG 2 cut(s) 986, 1518
BstSLI GKGCMC 1 cut(s) 1289
BstV1I GCAGC 2 cut(s) 1000, 1403
BstV2I GAAGAC 1 cut(s) 631
BstX2I RGATCY 2 cut(s) 10, 798
BstYI RGATCY 2 cut(s) 10, 798
Bsu36I CCTNAGG 1 cut(s) 1732
BsuRI GGCC 3 cut(s) 398, 467, 1102
BtgI CCRYGG 1 cut(s) 198
BtsCI GGATG 3 cut(s) 974, 1295, 1432
BtsI GCAGTG 2 cut(s) 1075, 1308
BtsIMutI CAGTG 6 cut(s) 22, 965, 1027, 1075, 1308, 1443
BveI ACCTGC 2 cut(s) 262, 1595
Cac8I GCNNGC 3 cut(s) 680, 811, 1625
CaiI CAGNNNCTG 4 cut(s) 227, 410, 1269, 1652
CfoI GCGC 1 cut(s) 1416
Cfr13I GGNCC 5 cut(s) 466, 1046, 1100, 1589, 1643
CseI GACGC 2 cut(s) 1512, 1694
Csp6I GTAC 3 cut(s) 690, 710, 835
CspCI CAANNNNNGTGG 2 cut(s) 1078, 1113
CviAII CATG 9 cut(s) 58, 161, 318, 534, 679, 685, 824, 1124, 1355
CviQI GTAC 3 cut(s) 690, 710, 835
DpnI GATC 8 cut(s) 12, 612, 618, 800, 1128, 1176, 1194, 1325
DpnII GATC 8 cut(s) 10, 610, 616, 798, 1126, 1174, 1192, 1323
EciI GGCGGA 1 cut(s) 1716
Ecl136II GAGCTC 1 cut(s) 422
Eco130I CCWWGG 1 cut(s) 383
Eco147I AGGCCT 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 424
Eco32I GATATC 3 cut(s) 1031, 1337, 1396
Eco47I GGWCC 3 cut(s) 1046, 1589, 1643
Eco53kI GAGCTC 1 cut(s) 422
Eco57I CTGAAG 1 cut(s) 1737
Eco81I CCTNAGG 1 cut(s) 1732
Eco88I CYCGRG 1 cut(s) 1512
EcoICRI GAGCTC 1 cut(s) 422
EcoRI GAATTC 1 cut(s) 596
EcoRII CCWGG 2 cut(s) 93, 1671
EcoRV GATATC 3 cut(s) 1031, 1337, 1396
EcoT14I CCWWGG 1 cut(s) 383
EcoT22I ATGCAT 1 cut(s) 63
EcoT38I GRGCYC 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 383
Esp3I CGTCTC 1 cut(s) 1530
FaeI CATG 9 cut(s) 61, 164, 321, 537, 682, 688, 827, 1127, 1358
FaqI GGGAC 2 cut(s) 1032, 1418
FatI CATG 9 cut(s) 57, 160, 317, 533, 678, 684, 823, 1123, 1354
FauI CCCGC 1 cut(s) 1192
FauNDI CATATG 1 cut(s) 576
FbaI TGATCA 2 cut(s) 1126, 1323
FblI GTMKAC 2 cut(s) 5, 903
Fnu4HI GCNGC 2 cut(s) 989, 1417
FokI GGATG 3 cut(s) 981, 1282, 1419
FriOI GRGCYC 1 cut(s) 424
Fsp4HI GCNGC 2 cut(s) 989, 1417
FspBI CTAG 1 cut(s) 470
GlaI GCGC 1 cut(s) 1415
GluI GCNGC 2 cut(s) 989, 1417
GsaI CCCAGC 1 cut(s) 1423
GsuI CTGGAG 1 cut(s) 1437
HaeIII GGCC 3 cut(s) 398, 467, 1102
HapII CCGG 1 cut(s) 1037
HgaI GACGC 2 cut(s) 1512, 1694
HhaI GCGC 1 cut(s) 1416
Hin1II CATG 9 cut(s) 61, 164, 321, 537, 682, 688, 827, 1127, 1358
Hin6I GCGC 1 cut(s) 1414
HinP1I GCGC 1 cut(s) 1414
HincII GTYRAC 2 cut(s) 6, 100
HindII GTYRAC 2 cut(s) 6, 100
HindIII AAGCTT 3 cut(s) 495, 941, 1595
HinfI GANTC 9 cut(s) 52, 203, 224, 548, 647, 655, 900, 974, 1255
HpaI GTTAAC 1 cut(s) 100
HpaII CCGG 1 cut(s) 1037
HphI GGTGA 4 cut(s) 574, 1435, 1609, 1697
Hpy166II GTNNAC 6 cut(s) 6, 100, 690, 904, 1287, 1360
Hpy188I TCNGA 8 cut(s) 208, 223, 795, 870, 899, 1156, 1225, 1381
Hpy8I GTNNAC 6 cut(s) 6, 100, 690, 904, 1287, 1360
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 6 cut(s) 32, 440, 642, 993, 1165, 1574
HpyCH4III ACNGT 2 cut(s) 1006, 1560
HpyF10VI GCNNNNNNNGC 5 cut(s) 140, 404, 659, 819, 1508
Hsp92II CATG 9 cut(s) 61, 164, 321, 537, 682, 688, 827, 1127, 1358
HspAI GCGC 1 cut(s) 1414
Ksp22I TGATCA 2 cut(s) 1126, 1323
KspAI GTTAAC 1 cut(s) 100
Kzo9I GATC 8 cut(s) 10, 610, 616, 798, 1126, 1174, 1192, 1323
LmnI GCTCC 5 cut(s) 157, 199, 1120, 1390, 1690
Lsp1109I GCAGC 2 cut(s) 1000, 1403
LweI GCATC 4 cut(s) 251, 553, 1206, 1372
MaeI CTAG 1 cut(s) 470
MaeIII GTNAC 2 cut(s) 1006, 1138
MalI GATC 8 cut(s) 12, 612, 618, 800, 1128, 1176, 1194, 1325
MboI GATC 8 cut(s) 10, 610, 616, 798, 1126, 1174, 1192, 1323
MflI RGATCY 2 cut(s) 10, 798
MhlI GDGCHC 3 cut(s) 424, 1289, 1695
MluCI AATT 7 cut(s) 268, 371, 596, 1085, 1229, 1504, 1662
MlyI GAGTC 2 cut(s) 909, 1264
Mph1103I ATGCAT 1 cut(s) 63
MroXI GAANNNNTTC 2 cut(s) 1085, 1491
MseI TTAA 5 cut(s) 99, 261, 416, 939, 1668
MslI CAYNNNNRTG 2 cut(s) 683, 1022
MspCI CTTAAG 2 cut(s) 415, 938
MspI CCGG 1 cut(s) 1037
MspR9I CCNGG 3 cut(s) 95, 1038, 1673
Mva1269I GAATGC 2 cut(s) 324, 1070
MvaI CCWGG 2 cut(s) 95, 1673
MwoI GCNNNNNNNGC 5 cut(s) 140, 404, 659, 819, 1508
NciI CCSGG 1 cut(s) 1038
NdeI CATATG 1 cut(s) 576
NdeII GATC 8 cut(s) 10, 610, 616, 798, 1126, 1174, 1192, 1323
NlaIII CATG 9 cut(s) 61, 164, 321, 537, 682, 688, 827, 1127, 1358
NlaIV GGNNCC 7 cut(s) 754, 800, 1048, 1488, 1562, 1590, 1644
NmeAIII GCCGAG 2 cut(s) 890, 1181
NmuCI GTSAC 1 cut(s) 1006
NsiI ATGCAT 1 cut(s) 63
NspI RCATGY 2 cut(s) 537, 682
OliI CACNNNNGTG 1 cut(s) 1022
PaeI GCATGC 1 cut(s) 682
PaeR7I CTCGAG 1 cut(s) 1512
PceI AGGCCT 1 cut(s) 398
PctI GAATGC 2 cut(s) 324, 1070
PdmI GAANNNNTTC 2 cut(s) 1085, 1491
PfeI GAWTC 7 cut(s) 52, 203, 224, 548, 647, 655, 974
PflMI CCANNNNNTGG 1 cut(s) 787
PkrI GCNGC 2 cut(s) 990, 1418
PleI GAGTC 2 cut(s) 908, 1263
PpsI GAGTC 2 cut(s) 908, 1263
PshBI ATTAAT 1 cut(s) 261
Psp124BI GAGCTC 1 cut(s) 424
Psp6I CCWGG 2 cut(s) 93, 1671
PspFI CCCAGC 1 cut(s) 1419
PspGI CCWGG 2 cut(s) 93, 1671
PspN4I GGNNCC 7 cut(s) 754, 800, 1048, 1488, 1562, 1590, 1644
PspPI GGNCC 5 cut(s) 466, 1046, 1100, 1589, 1643
PspXI VCTCGAGB 1 cut(s) 1512
PstI CTGCAG 1 cut(s) 990
PstNI CAGNNNCTG 4 cut(s) 227, 410, 1269, 1652
PsuI RGATCY 2 cut(s) 10, 798
RsaI GTAC 3 cut(s) 691, 711, 836
RsaNI GTAC 3 cut(s) 690, 710, 835
RseI CAYNNNNRTG 2 cut(s) 683, 1022
SacI GAGCTC 1 cut(s) 424
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 5 cut(s) 99, 261, 416, 939, 1668
SatI GCNGC 2 cut(s) 989, 1417
Sau3AI GATC 8 cut(s) 10, 610, 616, 798, 1126, 1174, 1192, 1323
Sau96I GGNCC 5 cut(s) 466, 1046, 1100, 1589, 1643
ScaI AGTACT 1 cut(s) 711
SchI GAGTC 2 cut(s) 909, 1264
ScrFI CCNGG 3 cut(s) 95, 1038, 1673
SduI GDGCHC 3 cut(s) 424, 1289, 1695
SfaNI GCATC 4 cut(s) 251, 553, 1206, 1372
SfcI CTRYAG 2 cut(s) 986, 1518
Sfr274I CTCGAG 1 cut(s) 1512
SinI GGWCC 3 cut(s) 1046, 1589, 1643
SlaI CTCGAG 1 cut(s) 1512
SmiMI CAYNNNNRTG 2 cut(s) 683, 1022
SmlI CTYRAG 8 cut(s) 306, 392, 415, 938, 1059, 1252, 1280, 1512
SmoI CTYRAG 8 cut(s) 306, 392, 415, 938, 1059, 1252, 1280, 1512
SphI GCATGC 1 cut(s) 682
Sse9I AATT 7 cut(s) 268, 371, 596, 1085, 1229, 1504, 1662
SseBI AGGCCT 1 cut(s) 398
SsiI CCGC 5 cut(s) 693, 717, 1185, 1299, 1727
SspI AATATT 1 cut(s) 776
SspMI CTAG 1 cut(s) 470
SstI GAGCTC 1 cut(s) 424
StuI AGGCCT 1 cut(s) 398
StyD4I CCNGG 3 cut(s) 93, 1036, 1671
StyI CCWWGG 1 cut(s) 383
TaaI ACNGT 2 cut(s) 1006, 1560
TaqI TCGA 4 cut(s) 5, 116, 600, 1513
TasI AATT 7 cut(s) 268, 371, 596, 1085, 1229, 1504, 1662
TatI WGTACW 1 cut(s) 709
TfiI GAWTC 7 cut(s) 52, 203, 224, 548, 647, 655, 974
Tru1I TTAA 5 cut(s) 99, 261, 416, 939, 1668
Tru9I TTAA 5 cut(s) 99, 261, 416, 939, 1668
TscAI CASTG 6 cut(s) 22, 965, 1027, 1075, 1308, 1443
TseFI GTSAC 1 cut(s) 1006
TseI GCWGC 2 cut(s) 988, 1416
Tsp45I GTSAC 1 cut(s) 1006
TspDTI ATGAA 8 cut(s) 13, 177, 264, 327, 938, 971, 1184, 1674
TspGWI ACGGA 4 cut(s) 23, 215, 713, 1301
TspRI CASTG 6 cut(s) 22, 965, 1027, 1075, 1308, 1443
Van91I CCANNNNNTGG 1 cut(s) 787
Vha464I CTTAAG 2 cut(s) 415, 938
VneI GTGCAC 1 cut(s) 1285
VpaK11BI GGWCC 3 cut(s) 1046, 1589, 1643
VspI ATTAAT 1 cut(s) 261
XapI RAATTY 5 cut(s) 268, 596, 1085, 1504, 1662
XceI RCATGY 2 cut(s) 537, 682
XcmI CCANNNNNNNNNTGG 2 cut(s) 1581, 1679
XhoI CTCGAG 1 cut(s) 1512
XmiI GTMKAC 2 cut(s) 5, 903
XmnI GAANNNNTTC 2 cut(s) 1085, 1491
XspI CTAG 1 cut(s) 470
ZrmI AGTACT 1 cut(s) 711
Zsp2I ATGCAT 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.