Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001945

Physical Location & Seq

Genomic Coordinates Forward (+)

75984517 .. 75993890

Gene Structure 9374 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021506

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2940 bp

ATGTCGACGGATCTCAGTGATTTCATCGTCATCAAAGAAGGAGAGGCTGAGATTCTCATGCATACTAAAAACCAAGTCTTTTACAACAAAACCCAGGTTAACAATAGAGACATCTCGATTGCTGTTATGAGGGCATTTGTAGCCAAACGCAAAGAGGAGCATGAAGCGAGATTGTCCAAGAAAAAAAAGACAGCTCCCACGGATTCTGAGAAAGATGTTTCAGAATCTGTTGTAGAAGAAGCATCTAATGAACCTGCTATTAATGGAAATTCCAATGGAGATTGTGAAACGCCAGAAGATATATCTCAAGATGAACCATGTAGCATTCCAGAAGAACCAAGCAAGACCACAGAAGGAAAAGGACGAGGGGAATTGAAGCCACCAAGAGTTCTTGAGCACAGGTATATCTATGGTGTCACTGGATTCATATCAGTTGAAGCTTGTAGGAGAAACATCAAATTCAATGGTTCAGTTGCATGTTCAAAGGTGGAATCACATCTTGTTGATGCTCGTGTGCATATGCTCACCCACCCAAAAGAATTTGATGTGGTTGATCTTGATCCTTATGGTTCTCCCTCTGTCTTCTTGGATTCTGCAATTCAAGCTGTTGCTGATGGAGGCATGCTCATGTGTACCGCAACGGATATGGCAGTACTATGTGGTGGTAATGGGGAGGTTTGCTATTCAAAATATGGTTCCTATCCATTGAAAGGGAAATATTGCCACGAAATGGCTCTGAGGATCCTCTTAGCCTGCATTGAGAGCCATGCAAATCGGTACAAAAGATACATTGTTCCTGTGCTATCTGTTCAGATGGACTTTTATGTTCGTGTTTTTGTCCGAGTCTACTCCTCGGCAAGTGCTATGAAGAACACTCCCCTTAAGCTTTCCTATGTTTATCAGTGCATTGGATGTGATTCTTTTCATCTGCAGCCTCTTGGAAGGACGGTCACTAAGAACACCAGTGTGAGATATCTTCCGGGTTTTGGTCCCATTGTTCCTCAAGAATGCAGTGATTGTGGGAAGAAATTCAATATGGGTGGGCCTGTATGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATACTATCAGATGTGAAATCAATGAAGGATCGTTATCCCGCTTATGATCGCATCTCGGCTGTACTGACAACAATCTCAGAGGAACTGCCAGATGTTCCTCTGTTCTTGAGTCTGCACAGCCTCTGTGCCACTCTCAAGTGCACTTCACCATCTGCAGTGATTTTCCGTTCTGCTGTGATCAATGCAGGATATCGTATATCTGGAACTCATGTAAACCCATTGGGGTTGAAGTCAGATGCTCCTATGGATATCATTTGGGACATAATGCGCTGCTGGGTGAAAAATCATCCAGTGAAAGCTCAACCTCCAGAACATTCAGGAAGCGTGATACTTGCTAAGGAACCTGTTCTTCAAGCAAATTTTGCTCGAGCTGTTGCATCTCTTAGCAAGGCACAGACCAAGAAGGTTGCACGGTTCCTTCCAAACCCGGAAAGGCATTGGGGTCCAAAGCTTAGGGCAGGTCGTCAAATCACCAGCAAGCACATTTCTCTCTTGGGTCCAGAGGCTGTCAATGAAATTCTTAACCAGGAAGAAGATGGTGAGGAACACAATGCCAAGCGTCAAAAGATTGAAGATAATCCGCCCTCAGATGCATATGATCCGCTTGATCCTAATGGCAACATCACAATCAAGTGGGATATTATAAGCTGGACACCTGACGGCTATGTTGCCGTTGTTACGCTTTTTAACTTCCAGCAGTATAGACACATTCAAGCACCAGGCTGGACGCTTGGATGGACATGGGCTAAGAAGGAGGTAATTTGGAACATGGTGGGAGGACAAGCCACCGATCAAGGTGATTGTTCAAAATTTAAGACAAATCCCCCACATTGCTGCAAAAAGACCCCAACCATTGTTGATCTACTCCCTGGAACTCCGTACAATCAGCAGTATTCCAATTGTTGTAGAGGGGGAGTGCTGAGCTCATGGATACAGGATCCATCCAATGCTGCAGCTTCTTTTCAACTAAGTGTTGGTCGATCTGGAACGACCAACAAGACAGTTAGGCTTCCGAAAAACTATACCCTAATGGGACCTGGGCCTGGGTACACTTGTGGTGTTGCAAAAGTTGTCAAACCAACTAAATTCTTTACAGCTGATAAAAGAAGAGTCTCTCAAGCCATGATGACATGGAATGTGACATGCACATATTCACAGTTCCTGGCTCAGAAAGCTCCTACTTGCTGTGTCTCGTTGTCTTCCTTCTACAATGACACAGTAGTGCCCTGCCCGACATGTTCGTGTGGCTGCCAAAACAACATAACTCATCCCGGAAGCTGTGTAGTGCCAGAGTCTCCGTATTTGGCTTCAGTTGTTTCAGCTGCTGATAAGAATTCACCTTTGGTTAGATGTACTAGCCATATGTGCCCTATCAGAGTTCATTGGCACGTCAAGCTGAATTACAAGGAATACTGGCGAGTGAAGGTCACAATTACAAACTTTGATTACAGGAGGAATTATTCTGATTGGAACTTGGTTGTCCAACACCCAAATTTTGACAATCTCACCCAAATTTTCAGCTTCAACGCAAAGTCCTTGACTCCTTATGGAACCATAAATGATACTGCAATGCTATGGGGAACCAAGTTCTACAACGACATGCTGGTGCAATCTGGGCCTCTCGGTAATGTGCAGTCAGAGATGCTTTTCCGGAAGAATCCAGCCACCTTCACTTTTGACAAGGGATGGGCGTTCCCAAGAAGGATCTATTTCAATGGTGATACTTGTGTAATGCCACCCCCAGATGCCTTTCCACATTTGCCAAATGCTGGTTTCCGACAGTATGTTTCTCTGCTTACTGTGATCATGATTTCAGTATCGACATTTGCATTCACATATGCCTATGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0002940 GO:0003674 GO:0003824 GO:0004809 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008175 GO:0008757 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016423 GO:0016740 GO:0016741 GO:0030488 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TRM

Protein Analysis

980

Amino Acids

109.13

Weight (kDa)

8.55

Isoelectric Point (pI)

38.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TRM	PF02005	145 - 453	1.9e-91	N2,N2-dimethylguanosine tRNA methyltransferase
COBRA	PF04833	585 - 748	2.2e-67	COBRA-like protein
COB_C	PF25079	757 - 948	2.3e-60	COBRA family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02320 AT5G15810
fragaria_vesca	FvH4_6g46450 FvH4_6g46450 FvH4_6g46450
malus_domestica	MD09G1073500.v1.1 MD17G1065000.v1.1
prunus_persica	Prupe.3G249800_v2.0.a1 Prupe.3G249800_v2.0.a1
pyrus_communis	pycom09g00090 pycom17g06500
rosa_chinensis	RchiOBHm_Chr2g0130081 RchiOBHm_Chr2g0165001
rosa_laevigata	RLG00000008503 RLG00000021506
rosa_multiflora	Rmu_co8255059.1_g000001 Rmu_sc0001097.1_g000019 Rmu_sc0002970.1_g000011 Rmu_sc0022378.1_g000001 Rmu_sc0026009.1_g000001
rosa_roxburghii	Rroxscaffold_1G00025420 Rroxscaffold_1G00038210 Rroxscaffold_1G00074000 Rroxscaffold_2G00083080 Rroxscaffold_5G00359260 Rroxscaffold_7G00169970 Rroxscaffold_7G00192270 Rroxscaffold_7G00205690
rosa_rugosa	Rorug02G0291000 Rorug02G0512100
rosa_samantha	Rh1AG215300 Rh1CG199800 Rh1DG211500 Rh2AG509800 Rh2AG580200 Rh2BG350400 Rh2BG572900 Rh2BG591500 Rh2CG495000 Rh2CG562000 Rh2DG601600 Rh4CG060300
rosa_wichuraiana	Rw0G021070 Rw2G027680 Rw2G048160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1733
Acc36I	ACCTGC	2 cut(s)	262, 1538
AccB7I	CCANNNNNTGG	2 cut(s)	730, 2858
AccI	GTMKAC	2 cut(s)	5, 846
AccIII	TCCGGA	1 cut(s)	2739
AciI	CCGC	4 cut(s)	636, 1128, 1670, 1691
AcuI	CTGAAG	1 cut(s)	2382
AfaI	GTAC	7 cut(s)	634, 654, 779, 1152, 1970, 2138, 2443
AfiI	CCNNNNNNNGG	5 cut(s)	710, 730, 986, 2132, 2858
AflII	CTTAAG	1 cut(s)	881
AflIII	ACRYGT	1 cut(s)	2324
AjiI	CACGTC	1 cut(s)	2479
AjnI	CCWGG	7 cut(s)	93, 1614, 1807, 1957, 2125, 2131, 2250
AjuI	GAANNNNNNNTTGG	2 cut(s)	2414, 2446
AleI	CACNNNNGTG	2 cut(s)	965, 2309
Alw21I	GWGCWC	3 cut(s)	399, 1232, 2015
Alw26I	GTCTC	4 cut(s)	102, 2206, 2284, 2388
Alw44I	GTGCAC	1 cut(s)	1228
AlwNI	CAGNNNCTG	5 cut(s)	227, 1212, 1595, 2251, 2414
Ama87I	CYCGRG	1 cut(s)	1455
Aor13HI	TCCGGA	1 cut(s)	2739
AoxI	GGCC	3 cut(s)	1043, 2129, 2705
ApaLI	GTGCAC	1 cut(s)	1228
ApeKI	GCWGC	7 cut(s)	931, 1359, 1923, 2039, 2042, 2337, 2411
AseI	ATTAAT	1 cut(s)	261
Asp700I	GAANNNNTTC	2 cut(s)	1028, 1434
AspLEI	GCGC	1 cut(s)	1359
AspS9I	GGNCC	7 cut(s)	989, 1043, 1532, 1586, 2123, 2129, 2705
AsuC2I	CCSGG	3 cut(s)	981, 1517, 2361
AsuHPI	GGTGA	9 cut(s)	517, 1227, 1378, 1552, 1640, 1898, 2418, 2587, 2819
AvaI	CYCGRG	1 cut(s)	1455
AvaII	GGWCC	4 cut(s)	989, 1532, 1586, 2123
BaeGI	GKGCMC	3 cut(s)	1232, 2316, 2459
BaeI	ACNNNNGTAYC	4 cut(s)	2128, 2161, 2802, 2835
BamHI	GGATCC	2 cut(s)	741, 2026
BanII	GRGCYC	1 cut(s)	2015
BauI	CACGAG	1 cut(s)	510
BbsI	GAAGAC	2 cut(s)	574, 2280
Bbv12I	GWGCWC	3 cut(s)	399, 1232, 2015
BbvI	GCAGC	7 cut(s)	943, 1346, 1910, 2026, 2054, 2324, 2398
BccI	CCATC	7 cut(s)	608, 808, 1246, 1619, 1818, 2038, 2769
BceAI	ACGGC	2 cut(s)	1745, 1765
BciT130I	CCWGG	7 cut(s)	95, 1616, 1809, 1959, 2127, 2133, 2252
BciVI	GTATCC	1 cut(s)	2013
BclI	TGATCA	3 cut(s)	1069, 1266, 2892
BcnI	CCSGG	3 cut(s)	981, 1517, 2361
BcoDI	GTCTC	4 cut(s)	102, 2206, 2284, 2388
BfaI	CTAG	1 cut(s)	2445
BfmI	CTRYAG	3 cut(s)	929, 1242, 2040
BfrI	CTTAAG	1 cut(s)	881
BfuAI	ACCTGC	2 cut(s)	262, 1538
BfuI	GTATCC	1 cut(s)	2013
BisI	GCNGC	7 cut(s)	932, 1360, 1924, 2040, 2043, 2338, 2412
BlpI	GCTNAGC	1 cut(s)	2009
BlsI	GCNGC	7 cut(s)	933, 1361, 1925, 2041, 2044, 2339, 2413
BmcAI	AGTACT	1 cut(s)	654
Bme18I	GGWCC	4 cut(s)	989, 1532, 1586, 2123
BmeT110I	CYCGRG	1 cut(s)	1455
BmgBI	CACGTC	1 cut(s)	2479
BmgT120I	GGNCC	7 cut(s)	989, 1043, 1532, 1586, 2123, 2129, 2705
BmsI	GCATC	8 cut(s)	251, 496, 1149, 1315, 1475, 1669, 2721, 2824
BpiI	GAAGAC	2 cut(s)	574, 2280
BplI	GAGNNNNNCTC	2 cut(s)	609, 641
BpmI	CTGGAG	1 cut(s)	1380
Bpu10I	CCTNAGC	2 cut(s)	1425, 1541
Bpu1102I	GCTNAGC	1 cut(s)	2009
BpuEI	CTTGAG	6 cut(s)	291, 413, 987, 1208, 1216, 2190
BpuMI	CCSGG	3 cut(s)	981, 1517, 2361
BsaBI	GATNNNNATC	3 cut(s)	427, 558, 1122
BsaJI	CCNNGG	6 cut(s)	93, 198, 852, 1957, 2126, 2132
BsaWI	WCCGGW	1 cut(s)	2739
Bsc4I	CCNNNNNNNGG	5 cut(s)	710, 730, 986, 2132, 2858
Bse1I	ACTGG	4 cut(s)	424, 963, 1379, 2507
Bse3DI	GCAATG	2 cut(s)	1918, 2664
Bse8I	GATNNNNATC	3 cut(s)	427, 558, 1122
BseAI	TCCGGA	1 cut(s)	2739
BseBI	CCWGG	7 cut(s)	95, 1616, 1809, 1959, 2127, 2133, 2252
BseDI	CCNNGG	6 cut(s)	93, 198, 852, 1957, 2126, 2132
BseGI	GGATG	6 cut(s)	917, 1375, 1829, 2030, 2356, 2780
BseJI	GATNNNNATC	3 cut(s)	427, 558, 1122
BseLI	CCNNNNNNNGG	5 cut(s)	710, 730, 986, 2132, 2858
BseMI	GCAATG	2 cut(s)	1918, 2664
BseMII	CTCAG	8 cut(s)	28, 39, 198, 728, 1179, 1689, 2000, 2270
BseNI	ACTGG	4 cut(s)	424, 963, 1379, 2507
BseRI	GAGGAG	2 cut(s)	170, 841
BseSI	GKGCMC	3 cut(s)	1232, 2316, 2459
BseXI	GCAGC	7 cut(s)	943, 1346, 1910, 2026, 2054, 2324, 2398
BseYI	CCCAGC	1 cut(s)	1362
BsgI	GTGCAG	2 cut(s)	1187, 2741
BshFI	GGCC	3 cut(s)	1045, 2131, 2707
BsiHKAI	GWGCWC	3 cut(s)	399, 1232, 2015
BsiHKCI	CYCGRG	1 cut(s)	1455
BsiSI	CCGG	4 cut(s)	980, 1517, 2361, 2740
BslFI	GGGAC	3 cut(s)	975, 1361, 2136
BslI	CCNNNNNNNGG	5 cut(s)	710, 730, 986, 2132, 2858
BsmAI	GTCTC	4 cut(s)	102, 2206, 2284, 2388
BsmFI	GGGAC	3 cut(s)	975, 1361, 2136
BsmI	GAATGC	3 cut(s)	324, 1013, 2918
BsnI	GGCC	3 cut(s)	1045, 2131, 2707
BsoBI	CYCGRG	1 cut(s)	1455
Bsp1286I	GDGCHC	5 cut(s)	399, 1232, 2015, 2316, 2459
Bsp13I	TCCGGA	1 cut(s)	2739
Bsp1720I	GCTNAGC	1 cut(s)	2009
BspACI	CCGC	4 cut(s)	636, 1128, 1670, 1691
BspANI	GGCC	3 cut(s)	1045, 2131, 2707
BspCNI	CTCAG	8 cut(s)	27, 40, 199, 729, 1178, 1688, 2001, 2269
BspEI	TCCGGA	1 cut(s)	2739
BspHI	TCATGA	1 cut(s)	2895
BspMAI	CTGCAG	3 cut(s)	933, 1246, 2044
BspMI	ACCTGC	2 cut(s)	262, 1538
BspTI	CTTAAG	1 cut(s)	881
BsrDI	GCAATG	2 cut(s)	1918, 2664
BsrI	ACTGG	4 cut(s)	424, 963, 1379, 2507
BssECI	CCNNGG	6 cut(s)	93, 198, 852, 1957, 2126, 2132
BssSI	CACGAG	1 cut(s)	510
Bst2BI	CACGAG	1 cut(s)	510
Bst2UI	CCWGG	7 cut(s)	95, 1616, 1809, 1959, 2127, 2133, 2252
Bst4CI	ACNGT	7 cut(s)	949, 1503, 2092, 2247, 2308, 2871, 2890
Bst6I	CTCTTC	1 cut(s)	2191
BstAFI	CTTAAG	1 cut(s)	881
BstAPI	GCANNNNNTGC	1 cut(s)	1451
BstC8I	GCNNGC	3 cut(s)	623, 754, 1568
BstDSI	CCRYGG	1 cut(s)	198
BstF5I	GGATG	6 cut(s)	917, 1375, 1829, 2030, 2356, 2780
BstHHI	GCGC	1 cut(s)	1359
BstMAI	GTCTC	4 cut(s)	102, 2206, 2284, 2388
BstMWI	GCNNNNNNNGC	8 cut(s)	140, 602, 762, 1451, 2261, 2454, 2482, 2704
BstNI	CCWGG	7 cut(s)	95, 1616, 1809, 1959, 2127, 2133, 2252
BstNSI	RCATGY	5 cut(s)	480, 625, 2235, 2328, 2692
BstSFI	CTRYAG	3 cut(s)	929, 1242, 2040
BstSLI	GKGCMC	3 cut(s)	1232, 2316, 2459
BstV1I	GCAGC	7 cut(s)	943, 1346, 1910, 2026, 2054, 2324, 2398
BstV2I	GAAGAC	2 cut(s)	574, 2280
BstX2I	RGATCY	4 cut(s)	10, 741, 2026, 2793
BstYI	RGATCY	4 cut(s)	10, 741, 2026, 2793
BsuI	GTATCC	1 cut(s)	2013
BsuRI	GGCC	3 cut(s)	1045, 2131, 2707
BtgI	CCRYGG	1 cut(s)	198
BtrI	CACGTC	1 cut(s)	2479
BtsCI	GGATG	6 cut(s)	917, 1375, 1829, 2030, 2356, 2780
BtsI	GCAGTG	2 cut(s)	1018, 1251
BtsIMutI	CAGTG	7 cut(s)	22, 417, 908, 970, 1018, 1251, 1386
BveI	ACCTGC	2 cut(s)	262, 1538
Cac8I	GCNNGC	3 cut(s)	623, 754, 1568
CaiI	CAGNNNCTG	5 cut(s)	227, 1212, 1595, 2251, 2414
CciI	TCATGA	1 cut(s)	2895
CfoI	GCGC	1 cut(s)	1359
Cfr13I	GGNCC	7 cut(s)	989, 1043, 1532, 1586, 2123, 2129, 2705
CseI	GACGC	2 cut(s)	1637, 1825
Csp6I	GTAC	7 cut(s)	633, 653, 778, 1151, 1969, 2137, 2442
CspCI	CAANNNNNGTGG	2 cut(s)	1021, 1056
CviQI	GTAC	7 cut(s)	633, 653, 778, 1151, 1969, 2137, 2442
Eam1104I	CTCTTC	1 cut(s)	2191
EarI	CTCTTC	1 cut(s)	2191
EciI	GGCGGA	1 cut(s)	1659
Ecl136II	GAGCTC	1 cut(s)	2013
Eco24I	GRGCYC	1 cut(s)	2015
Eco32I	GATATC	3 cut(s)	974, 1280, 1339
Eco47I	GGWCC	4 cut(s)	989, 1532, 1586, 2123
Eco53kI	GAGCTC	1 cut(s)	2013
Eco57I	CTGAAG	1 cut(s)	2382
Eco88I	CYCGRG	1 cut(s)	1455
EcoICRI	GAGCTC	1 cut(s)	2013
EcoO109I	RGGNCCY	1 cut(s)	2123
EcoRI	GAATTC	1 cut(s)	2422
EcoRII	CCWGG	7 cut(s)	93, 1614, 1807, 1957, 2125, 2131, 2250
EcoRV	GATATC	3 cut(s)	974, 1280, 1339
EcoT22I	ATGCAT	2 cut(s)	63, 1684
EcoT38I	GRGCYC	1 cut(s)	2015
FaqI	GGGAC	3 cut(s)	975, 1361, 2136
FauI	CCCGC	1 cut(s)	1135
FauNDI	CATATG	4 cut(s)	519, 1684, 2451, 2926
FbaI	TGATCA	3 cut(s)	1069, 1266, 2892
FblI	GTMKAC	2 cut(s)	5, 846
Fnu4HI	GCNGC	7 cut(s)	932, 1360, 1924, 2040, 2043, 2338, 2412
FokI	GGATG	6 cut(s)	924, 1362, 1836, 2017, 2343, 2787
FriOI	GRGCYC	1 cut(s)	2015
Fsp4HI	GCNGC	7 cut(s)	932, 1360, 1924, 2040, 2043, 2338, 2412
FspBI	CTAG	1 cut(s)	2445
GlaI	GCGC	1 cut(s)	1358
GluI	GCNGC	7 cut(s)	932, 1360, 1924, 2040, 2043, 2338, 2412
GsaI	CCCAGC	1 cut(s)	1366
GsuI	CTGGAG	1 cut(s)	1380
HaeIII	GGCC	3 cut(s)	1045, 2131, 2707
HapII	CCGG	4 cut(s)	980, 1517, 2361, 2740
HgaI	GACGC	2 cut(s)	1637, 1825
HhaI	GCGC	1 cut(s)	1359
Hin6I	GCGC	1 cut(s)	1357
HinP1I	GCGC	1 cut(s)	1357
HincII	GTYRAC	2 cut(s)	6, 100
HindII	GTYRAC	2 cut(s)	6, 100
HindIII	AAGCTT	3 cut(s)	438, 884, 1538
HpaI	GTTAAC	1 cut(s)	100
HpaII	CCGG	4 cut(s)	980, 1517, 2361, 2740
HphI	GGTGA	9 cut(s)	517, 1227, 1378, 1552, 1640, 1898, 2418, 2587, 2819
Hpy166II	GTNNAC	7 cut(s)	6, 100, 633, 847, 1230, 1303, 2139
Hpy8I	GTNNAC	7 cut(s)	6, 100, 633, 847, 1230, 1303, 2139
Hpy99I	CGWCG	1 cut(s)	10
HpyCH4III	ACNGT	7 cut(s)	949, 1503, 2092, 2247, 2308, 2871, 2890
HpyCH4IV	ACGT	1 cut(s)	2478
HpyF10VI	GCNNNNNNNGC	8 cut(s)	140, 602, 762, 1451, 2261, 2454, 2482, 2704
HpySE526I	ACGT	1 cut(s)	2478
HspAI	GCGC	1 cut(s)	1357
Kpn2I	TCCGGA	1 cut(s)	2739
Ksp22I	TGATCA	3 cut(s)	1069, 1266, 2892
KspAI	GTTAAC	1 cut(s)	100
LmnI	GCTCC	5 cut(s)	157, 199, 1063, 1333, 2269
Lsp1109I	GCAGC	7 cut(s)	943, 1346, 1910, 2026, 2054, 2324, 2398
LweI	GCATC	8 cut(s)	251, 496, 1149, 1315, 1475, 1669, 2721, 2824
MaeI	CTAG	1 cut(s)	2445
MaeII	ACGT	1 cut(s)	2478
MaeIII	GTNAC	6 cut(s)	415, 949, 1081, 1765, 2227, 2515
MfeI	CAATTG	1 cut(s)	1987
MflI	RGATCY	4 cut(s)	10, 741, 2026, 2793
MhlI	GDGCHC	5 cut(s)	399, 1232, 2015, 2316, 2459
MlyI	GAGTC	5 cut(s)	852, 1207, 2208, 2390, 2623
MmeI	TCCRAC	2 cut(s)	2596, 2890
Mph1103I	ATGCAT	2 cut(s)	63, 1684
MroI	TCCGGA	1 cut(s)	2739
MroXI	GAANNNNTTC	2 cut(s)	1028, 1434
MseI	TTAA	6 cut(s)	99, 261, 882, 1611, 1776, 1902
MslI	CAYNNNNRTG	5 cut(s)	626, 965, 2309, 2329, 2693
MspA1I	CMGCKG	2 cut(s)	2186, 2411
MspCI	CTTAAG	1 cut(s)	881
MspI	CCGG	4 cut(s)	980, 1517, 2361, 2740
MunI	CAATTG	1 cut(s)	1987
Mva1269I	GAATGC	3 cut(s)	324, 1013, 2918
MvaI	CCWGG	7 cut(s)	95, 1616, 1809, 1959, 2127, 2133, 2252
MwoI	GCNNNNNNNGC	8 cut(s)	140, 602, 762, 1451, 2261, 2454, 2482, 2704
NciI	CCSGG	3 cut(s)	981, 1517, 2361
NdeI	CATATG	4 cut(s)	519, 1684, 2451, 2926
NmeAIII	GCCGAG	2 cut(s)	833, 1124
NmuCI	GTSAC	4 cut(s)	415, 949, 2227, 2515
NsiI	ATGCAT	2 cut(s)	63, 1684
NspI	RCATGY	5 cut(s)	480, 625, 2235, 2328, 2692
OliI	CACNNNNGTG	2 cut(s)	965, 2309
PaeI	GCATGC	1 cut(s)	625
PaeR7I	CTCGAG	1 cut(s)	1455
PagI	TCATGA	1 cut(s)	2895
PciI	ACATGT	1 cut(s)	2324
PctI	GAATGC	3 cut(s)	324, 1013, 2918
PdmI	GAANNNNTTC	2 cut(s)	1028, 1434
PfeI	GAWTC	8 cut(s)	52, 203, 224, 423, 491, 590, 917, 2746
PflMI	CCANNNNNTGG	2 cut(s)	730, 2858
PfoI	TCCNGGA	1 cut(s)	2359
PkrI	GCNGC	7 cut(s)	933, 1361, 1925, 2041, 2044, 2339, 2413
PleI	GAGTC	5 cut(s)	851, 1206, 2207, 2389, 2623
PpsI	GAGTC	5 cut(s)	851, 1206, 2207, 2389, 2623
PpuMI	RGGWCCY	1 cut(s)	2123
PscI	ACATGT	1 cut(s)	2324
PshBI	ATTAAT	1 cut(s)	261
PsiI	TTATAA	1 cut(s)	1733
Psp124BI	GAGCTC	1 cut(s)	2015
Psp5II	RGGWCCY	1 cut(s)	2123
Psp6I	CCWGG	7 cut(s)	93, 1614, 1807, 1957, 2125, 2131, 2250
PspFI	CCCAGC	1 cut(s)	1362
PspGI	CCWGG	7 cut(s)	93, 1614, 1807, 1957, 2125, 2131, 2250
PspPI	GGNCC	7 cut(s)	989, 1043, 1532, 1586, 2123, 2129, 2705
PspPPI	RGGWCCY	1 cut(s)	2123
PspXI	VCTCGAGB	1 cut(s)	1455
PstI	CTGCAG	3 cut(s)	933, 1246, 2044
PstNI	CAGNNNCTG	5 cut(s)	227, 1212, 1595, 2251, 2414
PsuI	RGATCY	4 cut(s)	10, 741, 2026, 2793
PvuII	CAGCTG	2 cut(s)	2186, 2411
RsaI	GTAC	7 cut(s)	634, 654, 779, 1152, 1970, 2138, 2443
RsaNI	GTAC	7 cut(s)	633, 653, 778, 1151, 1969, 2137, 2442
RseI	CAYNNNNRTG	5 cut(s)	626, 965, 2309, 2329, 2693
SacI	GAGCTC	1 cut(s)	2015
SalI	GTCGAC	1 cut(s)	4
SaqAI	TTAA	6 cut(s)	99, 261, 882, 1611, 1776, 1902
SatI	GCNGC	7 cut(s)	932, 1360, 1924, 2040, 2043, 2338, 2412
Sau96I	GGNCC	7 cut(s)	989, 1043, 1532, 1586, 2123, 2129, 2705
ScaI	AGTACT	1 cut(s)	654
SchI	GAGTC	5 cut(s)	852, 1207, 2208, 2390, 2623
SduI	GDGCHC	5 cut(s)	399, 1232, 2015, 2316, 2459
SfaNI	GCATC	8 cut(s)	251, 496, 1149, 1315, 1475, 1669, 2721, 2824
SfcI	CTRYAG	3 cut(s)	929, 1242, 2040
Sfr274I	CTCGAG	1 cut(s)	1455
SinI	GGWCC	4 cut(s)	989, 1532, 1586, 2123
SlaI	CTCGAG	1 cut(s)	1455
SmiMI	CAYNNNNRTG	5 cut(s)	626, 965, 2309, 2329, 2693
SmlI	CTYRAG	8 cut(s)	306, 392, 881, 1002, 1195, 1223, 1455, 2205
SmoI	CTYRAG	8 cut(s)	306, 392, 881, 1002, 1195, 1223, 1455, 2205
SphI	GCATGC	1 cut(s)	625
SsiI	CCGC	4 cut(s)	636, 1128, 1670, 1691
SspI	AATATT	1 cut(s)	719
SspMI	CTAG	1 cut(s)	2445
SstI	GAGCTC	1 cut(s)	2015
TaaI	ACNGT	7 cut(s)	949, 1503, 2092, 2247, 2308, 2871, 2890
TaiI	ACGT	1 cut(s)	2481
TaqI	TCGA	5 cut(s)	5, 116, 1456, 2068, 2909
TatI	WGTACW	3 cut(s)	652, 1150, 2441
TfiI	GAWTC	8 cut(s)	52, 203, 224, 423, 491, 590, 917, 2746
Tru1I	TTAA	6 cut(s)	99, 261, 882, 1611, 1776, 1902
Tru9I	TTAA	6 cut(s)	99, 261, 882, 1611, 1776, 1902
TscAI	CASTG	7 cut(s)	22, 424, 908, 970, 1018, 1251, 1386
TseFI	GTSAC	4 cut(s)	415, 949, 2227, 2515
TseI	GCWGC	7 cut(s)	931, 1359, 1923, 2039, 2042, 2337, 2411
Tsp45I	GTSAC	4 cut(s)	415, 949, 2227, 2515
TspGWI	ACGGA	6 cut(s)	23, 215, 656, 1244, 1956, 2376
TspRI	CASTG	7 cut(s)	22, 424, 908, 970, 1018, 1251, 1386
Van91I	CCANNNNNTGG	2 cut(s)	730, 2858
Vha464I	CTTAAG	1 cut(s)	881
VneI	GTGCAC	1 cut(s)	1228
VpaK11BI	GGWCC	4 cut(s)	989, 1532, 1586, 2123
VspI	ATTAAT	1 cut(s)	261
XceI	RCATGY	5 cut(s)	480, 625, 2235, 2328, 2692
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1622
XhoI	CTCGAG	1 cut(s)	1455
XmiI	GTMKAC	2 cut(s)	5, 846
XmnI	GAANNNNTTC	2 cut(s)	1028, 1434
XspI	CTAG	1 cut(s)	2445
ZrmI	AGTACT	1 cut(s)	654
Zsp2I	ATGCAT	2 cut(s)	63, 1684

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.