Rh2AG509800

Arsenical pump-driving

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001945
Physical Location & Seq
Forward (+)
73514286 .. 73536062
21777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG509800.1

Sequence Viewer

Length: 522 bp
ATGGGAGCACCTGCAAAAGATGCGCGTTTGGAGCCCCTTTGCGCAGAAGCCTGGTTTGGGCTAGCAATGGTTGATCTTGATCCTTATGGTTCTCCCTCCGTCTTCTTGGATTCTGCGATTCAAGCTGTTGCTGATGGAGGCATGCTCATGTGTACCGCAACGGATATGCCAGTACTATGTGCTGGTAATGGGGAGGTGATACAATTTCTCGAATCCCCAGAATATAACATGTTTACTTGTATCGTCTTTGATACTGCACCCACGGGCCATACCTTGCGACTTTTATCCTTGCCAGACTTCTTGGATGCATCAATTGGCAAGATATTGAAGTCAGACAAATTAGAGAAATTACGGGAGAGGATGATTAAAGTGCATGAGCTTTTTCGTGATACCGATTCCACAGAATTTGTCATAGTAACAATACCCACGTTACTTTTAAAGTCAGTGCAAGAGAATGATAACTATGCTGCAATGAGAGAAAAGCCCAAAGATTCTCCAAGTGGAAGCATAGCAAGAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArsA_ATPase

Protein Analysis

173

Amino Acids

18.9

Weight (kDa)

4.65

Isoelectric Point (pI)

29.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRM PF02005 21 - 62 3.2e-14 N2,N2-dimethylguanosine tRNA methyltransferase
ArsA_ATPase PF02374 66 - 122 1.3e-11 Anion-transporting ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 19
Acc16I TGCGCA 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 19
AccII CGCG 1 cut(s) 25
AciI CCGC 1 cut(s) 156
AclWI GGATC 1 cut(s) 74
AcsI RAATTY 1 cut(s) 404
AfaI GTAC 2 cut(s) 154, 174
AfiI CCNNNNNNNGG 1 cut(s) 57
AflIII ACRYGT 1 cut(s) 228
AgsI TTSAA 2 cut(s) 122, 328
AjnI CCWGG 1 cut(s) 50
AjuI GAANNNNNNNTTGG 2 cut(s) 39, 71
AluBI AGCT 3 cut(s) 125, 379, 518
AluI AGCT 3 cut(s) 125, 379, 518
Alw21I GWGCWC 1 cut(s) 10
AlwI GGATC 1 cut(s) 74
AoxI GGCC 1 cut(s) 265
ApeKI GCWGC 1 cut(s) 467
ApoI RAATTY 1 cut(s) 404
AspLEI GCGC 2 cut(s) 25, 44
AspS9I GGNCC 1 cut(s) 265
AsuHPI GGTGA 1 cut(s) 208
AsuNHI GCTAGC 1 cut(s) 61
BanII GRGCYC 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 10
BbvI GCAGC 1 cut(s) 454
BccI CCATC 1 cut(s) 128
BciT130I CCWGG 1 cut(s) 52
BfaI CTAG 1 cut(s) 62
BfuAI ACCTGC 1 cut(s) 19
BisI GCNGC 1 cut(s) 468
BlsI GCNGC 1 cut(s) 469
BmcAI AGTACT 1 cut(s) 174
Bme1390I CCNGG 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 1 cut(s) 33
BmrFI CCNGG 1 cut(s) 52
BmsI GCATC 3 cut(s) 10, 295, 317
BmtI GCTAGC 1 cut(s) 65
BpiI GAAGAC 1 cut(s) 94
BplI GAGNNNNNCTC 2 cut(s) 129, 161
BsaBI GATNNNNATC 1 cut(s) 78
BsaJI CCNNGG 1 cut(s) 261
Bsc4I CCNNNNNNNGG 1 cut(s) 57
Bse1I ACTGG 1 cut(s) 170
Bse3DI GCAATG 2 cut(s) 72, 477
Bse8I GATNNNNATC 1 cut(s) 78
BseBI CCWGG 1 cut(s) 52
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 2 cut(s) 310, 366
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 1 cut(s) 57
BseMI GCAATG 2 cut(s) 72, 477
BseNI ACTGG 1 cut(s) 170
BseXI GCAGC 1 cut(s) 454
BsgI GTGCAG 1 cut(s) 240
Bsh1236I CGCG 1 cut(s) 25
BshFI GGCC 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 10
BslI CCNNNNNNNGG 1 cut(s) 57
BsnI GGCC 1 cut(s) 267
Bsp1286I GDGCHC 2 cut(s) 10, 36
Bsp143I GATC 2 cut(s) 73, 79
BspACI CCGC 1 cut(s) 156
BspANI GGCC 1 cut(s) 267
BspFNI CGCG 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 33
BspMI ACCTGC 1 cut(s) 19
BspOI GCTAGC 1 cut(s) 65
BspPI GGATC 1 cut(s) 74
BsrDI GCAATG 2 cut(s) 72, 477
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 2 cut(s) 73, 79
Bst2UI CCWGG 1 cut(s) 52
BstAPI GCANNNNNTGC 1 cut(s) 20
BstC8I GCNNGC 2 cut(s) 63, 143
BstDSI CCRYGG 1 cut(s) 261
BstF5I GGATG 2 cut(s) 310, 366
BstFNI CGCG 1 cut(s) 25
BstHHI GCGC 2 cut(s) 25, 44
BstKTI GATC 2 cut(s) 76, 82
BstMBI GATC 2 cut(s) 73, 79
BstMWI GCNNNNNNNGC 3 cut(s) 20, 31, 122
BstNI CCWGG 1 cut(s) 52
BstNSI RCATGY 2 cut(s) 145, 232
BstSCI CCNGG 1 cut(s) 50
BstUI CGCG 1 cut(s) 25
BstV1I GCAGC 1 cut(s) 454
BstV2I GAAGAC 1 cut(s) 94
BsuRI GGCC 1 cut(s) 267
BtgI CCRYGG 1 cut(s) 261
BtsCI GGATG 2 cut(s) 310, 366
BtsIMutI CAGTG 1 cut(s) 450
BveI ACCTGC 1 cut(s) 19
Cac8I GCNNGC 2 cut(s) 63, 143
CfoI GCGC 2 cut(s) 25, 44
Cfr13I GGNCC 1 cut(s) 265
Csp6I GTAC 2 cut(s) 153, 173
CspCI CAANNNNNGTGG 2 cut(s) 388, 423
CviAII CATG 4 cut(s) 142, 148, 229, 374
CviJI RGCY 8 cut(s) 34, 50, 61, 125, 267, 379, 484, 518
CviKI_1 RGCY 8 cut(s) 34, 50, 61, 125, 267, 379, 484, 518
CviQI GTAC 2 cut(s) 153, 173
DpnI GATC 2 cut(s) 75, 81
DpnII GATC 2 cut(s) 73, 79
DraI TTTAAA 1 cut(s) 438
Eco24I GRGCYC 1 cut(s) 36
EcoRII CCWGG 1 cut(s) 50
EcoT22I ATGCAT 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 36
FaeI CATG 4 cut(s) 145, 151, 232, 377
FatI CATG 4 cut(s) 141, 147, 228, 373
Fnu4HI GCNGC 1 cut(s) 468
FokI GGATG 2 cut(s) 317, 373
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 1 cut(s) 468
FspBI CTAG 1 cut(s) 62
FspI TGCGCA 1 cut(s) 43
GlaI GCGC 2 cut(s) 24, 43
GluI GCNGC 1 cut(s) 468
HaeIII GGCC 1 cut(s) 267
HhaI GCGC 2 cut(s) 25, 44
Hin1II CATG 4 cut(s) 145, 151, 232, 377
Hin6I GCGC 2 cut(s) 23, 42
HinP1I GCGC 2 cut(s) 23, 42
HinfI GANTC 5 cut(s) 110, 118, 212, 395, 491
HphI GGTGA 1 cut(s) 208
Hpy166II GTNNAC 2 cut(s) 153, 234
Hpy188I TCNGA 1 cut(s) 334
Hpy188III TCNNGA 3 cut(s) 77, 209, 386
Hpy8I GTNNAC 2 cut(s) 153, 234
HpyCH4IV ACGT 1 cut(s) 428
HpyCH4V TGCA 6 cut(s) 14, 257, 308, 373, 448, 470
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 31, 122
HpySE526I ACGT 1 cut(s) 428
Hsp92II CATG 4 cut(s) 145, 151, 232, 377
HspAI GCGC 2 cut(s) 23, 42
Kzo9I GATC 2 cut(s) 73, 79
LmnI GCTCC 2 cut(s) 5, 31
LpnPI CCDG 7 cut(s) 24, 37, 64, 168, 183, 231, 306
Lsp1109I GCAGC 1 cut(s) 454
LweI GCATC 3 cut(s) 10, 295, 317
MaeI CTAG 1 cut(s) 62
MaeII ACGT 1 cut(s) 428
MaeIII GTNAC 2 cut(s) 415, 429
MalI GATC 2 cut(s) 75, 81
MboI GATC 2 cut(s) 73, 79
MboII GAAGA 1 cut(s) 94
MfeI CAATTG 1 cut(s) 312
MhlI GDGCHC 2 cut(s) 10, 36
MluCI AATT 5 cut(s) 203, 312, 338, 347, 404
MnlI CCTC 4 cut(s) 106, 131, 187, 351
Mph1103I ATGCAT 1 cut(s) 310
MseI TTAA 3 cut(s) 366, 437, 520
MslI CAYNNNNRTG 1 cut(s) 146
MspR9I CCNGG 1 cut(s) 52
MunI CAATTG 1 cut(s) 312
MvaI CCWGG 1 cut(s) 52
MvnI CGCG 1 cut(s) 25
MwoI GCNNNNNNNGC 3 cut(s) 20, 31, 122
NdeII GATC 2 cut(s) 73, 79
NheI GCTAGC 1 cut(s) 61
NlaIII CATG 4 cut(s) 145, 151, 232, 377
NlaIV GGNNCC 1 cut(s) 33
NsbI TGCGCA 1 cut(s) 43
NsiI ATGCAT 1 cut(s) 310
NspI RCATGY 2 cut(s) 145, 232
PaeI GCATGC 1 cut(s) 145
PaqCI CACCTGC 1 cut(s) 19
PciI ACATGT 1 cut(s) 228
PfeI GAWTC 5 cut(s) 110, 118, 212, 395, 491
PkrI GCNGC 1 cut(s) 469
PscI ACATGT 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 50
PspN4I GGNNCC 1 cut(s) 33
PspPI GGNCC 1 cut(s) 265
RsaI GTAC 2 cut(s) 154, 174
RsaNI GTAC 2 cut(s) 153, 173
RseI CAYNNNNRTG 1 cut(s) 146
SaqAI TTAA 3 cut(s) 366, 437, 520
SatI GCNGC 1 cut(s) 468
Sau3AI GATC 2 cut(s) 73, 79
Sau96I GGNCC 1 cut(s) 265
ScaI AGTACT 1 cut(s) 174
ScrFI CCNGG 1 cut(s) 52
SduI GDGCHC 2 cut(s) 10, 36
SetI ASST 7 cut(s) 13, 127, 198, 275, 381, 431, 520
SfaNI GCATC 3 cut(s) 10, 295, 317
SmiMI CAYNNNNRTG 1 cut(s) 146
SphI GCATGC 1 cut(s) 145
Sse9I AATT 5 cut(s) 203, 312, 338, 347, 404
SsiI CCGC 1 cut(s) 156
SspMI CTAG 1 cut(s) 62
StyD4I CCNGG 1 cut(s) 50
TaiI ACGT 1 cut(s) 431
TaqI TCGA 1 cut(s) 210
TasI AATT 5 cut(s) 203, 312, 338, 347, 404
TatI WGTACW 1 cut(s) 172
TfiI GAWTC 5 cut(s) 110, 118, 212, 395, 491
Tru1I TTAA 3 cut(s) 366, 437, 520
Tru9I TTAA 3 cut(s) 366, 437, 520
TscAI CASTG 1 cut(s) 450
TseI GCWGC 1 cut(s) 467
TspGWI ACGGA 2 cut(s) 88, 176
TspRI CASTG 1 cut(s) 450
XapI RAATTY 1 cut(s) 404
XceI RCATGY 2 cut(s) 145, 232
XspI CTAG 1 cut(s) 62
ZrmI AGTACT 1 cut(s) 174
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.