tRNA (Guanine(26)-N(2))-dimethyltransferase
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G02320 AT5G15810 |
| fragaria_vesca | FvH4_6g46450 FvH4_6g46450 FvH4_6g46450 |
| malus_domestica | MD09G1073500.v1.1 MD17G1065000.v1.1 |
| prunus_persica | Prupe.3G249800_v2.0.a1 Prupe.3G249800_v2.0.a1 |
| pyrus_communis | pycom09g00090 pycom17g06500 |
| rosa_chinensis | RchiOBHm_Chr2g0130081 RchiOBHm_Chr2g0165001 |
| rosa_laevigata | RLG00000008503 RLG00000021506 |
| rosa_multiflora | Rmu_co8255059.1_g000001 Rmu_sc0001097.1_g000019 Rmu_sc0002970.1_g000011 Rmu_sc0022378.1_g000001 Rmu_sc0026009.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00025420 Rroxscaffold_1G00038210 Rroxscaffold_1G00074000 Rroxscaffold_2G00083080 Rroxscaffold_5G00359260 Rroxscaffold_7G00169970 Rroxscaffold_7G00192270 Rroxscaffold_7G00205690 |
| rosa_rugosa | Rorug02G0291000 Rorug02G0512100 |
| rosa_samantha | Rh1AG215300 Rh1CG199800 Rh1DG211500 Rh2AG509800 Rh2AG580200 Rh2BG350400 Rh2BG572900 Rh2BG591500 Rh2CG495000 Rh2CG562000 Rh2DG601600 Rh4CG060300 |
| rosa_wichuraiana | Rw0G021070 Rw2G027680 Rw2G048160 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1598 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 790 |
| AccBSI | CCGCTC | 1 cut(s) | 1180 |
| AccII | CGCG | 1 cut(s) | 441 |
| AccIII | TCCGGA | 2 cut(s) | 1349, 1576 |
| AciI | CCGC | 3 cut(s) | 122, 656, 1180 |
| AclWI | GGATC | 4 cut(s) | 614, 796, 809, 1471 |
| AcsI | RAATTY | 4 cut(s) | 223, 486, 599, 1088 |
| AcuI | CTGAAG | 3 cut(s) | 398, 974, 1304 |
| AdeI | CACNNNGTG | 1 cut(s) | 1441 |
| AfaI | GTAC | 4 cut(s) | 694, 848, 1212, 1291 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 770, 790, 1046, 1486, 1681 |
| AflII | CTTAAG | 1 cut(s) | 941 |
| AgsI | TTSAA | 9 cut(s) | 143, 222, 497, 523, 543, 662, 747, 1093, 1718 |
| AleI | CACNNNNGTG | 1 cut(s) | 1285 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1482, 1514 |
| AluBI | AGCT | 5 cut(s) | 425, 500, 946, 1505, 1600 |
| AluI | AGCT | 5 cut(s) | 425, 500, 946, 1505, 1600 |
| Alw21I | GWGCWC | 1 cut(s) | 427 |
| Alw26I | GTCTC | 1 cut(s) | 1533 |
| AlwI | GGATC | 4 cut(s) | 614, 796, 809, 1471 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 701, 1655 |
| Aor13HI | TCCGGA | 2 cut(s) | 1349, 1576 |
| AoxI | GGCC | 3 cut(s) | 399, 1103, 1591 |
| ApaI | GGGCCC | 2 cut(s) | 1107, 1595 |
| ApeKI | GCWGC | 2 cut(s) | 296, 324 |
| ApoI | RAATTY | 4 cut(s) | 223, 486, 599, 1088 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1088, 1494 |
| AspLEI | GCGC | 1 cut(s) | 1419 |
| AspS9I | GGNCC | 6 cut(s) | 1049, 1103, 1104, 1591, 1592, 1646 |
| AsuC2I | CCSGG | 1 cut(s) | 1041 |
| AsuHPI | GGTGA | 4 cut(s) | 577, 1427, 1438, 1612 |
| AvaII | GGWCC | 2 cut(s) | 1049, 1646 |
| BaeGI | GKGCMC | 2 cut(s) | 1107, 1595 |
| BamHI | GGATCC | 1 cut(s) | 801 |
| BanII | GRGCYC | 3 cut(s) | 427, 1107, 1595 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 895, 927 |
| BauI | CACGAG | 1 cut(s) | 570 |
| BbsI | GAAGAC | 1 cut(s) | 634 |
| Bbv12I | GWGCWC | 1 cut(s) | 427 |
| BbvCI | CCTCAGC | 1 cut(s) | 1449 |
| BbvI | GCAGC | 2 cut(s) | 308, 336 |
| BccI | CCATC | 3 cut(s) | 35, 868, 1306 |
| BceAI | ACGGC | 1 cut(s) | 1505 |
| BciVI | GTATCC | 1 cut(s) | 1194 |
| BclI | TGATCA | 2 cut(s) | 1129, 1326 |
| BcnI | CCSGG | 1 cut(s) | 1041 |
| BcoDI | GTCTC | 1 cut(s) | 1533 |
| BfaI | CTAG | 3 cut(s) | 473, 809, 1515 |
| BfmI | CTRYAG | 1 cut(s) | 1302 |
| BfrI | CTTAAG | 1 cut(s) | 941 |
| BfuAI | ACCTGC | 1 cut(s) | 1598 |
| BfuI | GTATCC | 1 cut(s) | 1194 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 407 |
| BisI | GCNGC | 2 cut(s) | 297, 325 |
| BlsI | GCNGC | 2 cut(s) | 298, 326 |
| Bme1390I | CCNGG | 1 cut(s) | 1041 |
| Bme18I | GGWCC | 2 cut(s) | 1049, 1646 |
| BmgT120I | GGNCC | 6 cut(s) | 1049, 1103, 1104, 1591, 1592, 1646 |
| BmiI | GGNNCC | 9 cut(s) | 318, 757, 803, 1105, 1471, 1491, 1592, 1593, 1647 |
| BmrFI | CCNGG | 1 cut(s) | 1041 |
| BmrI | ACTGGG | 2 cut(s) | 1433, 1597 |
| BmsI | GCATC | 5 cut(s) | 48, 205, 556, 1209, 1375 |
| BmuI | ACTGGG | 2 cut(s) | 1433, 1597 |
| BpiI | GAAGAC | 1 cut(s) | 634 |
| BpmI | CTGGAG | 1 cut(s) | 1440 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1449, 1485, 1601 |
| BpuEI | CTTGAG | 4 cut(s) | 294, 416, 1047, 1276 |
| BpuMI | CCSGG | 1 cut(s) | 1041 |
| BsaBI | GATNNNNATC | 1 cut(s) | 618 |
| BsaJI | CCNNGG | 3 cut(s) | 1040, 1368, 1392 |
| BsaWI | WCCGGW | 2 cut(s) | 1349, 1576 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1482, 1512 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 770, 790, 1046, 1486, 1681 |
| Bse1I | ACTGG | 2 cut(s) | 1439, 1592 |
| Bse3DI | GCAATG | 2 cut(s) | 930, 1078 |
| Bse8I | GATNNNNATC | 1 cut(s) | 618 |
| BseAI | TCCGGA | 2 cut(s) | 1349, 1576 |
| BseDI | CCNNGG | 3 cut(s) | 1040, 1368, 1392 |
| BseGI | GGATG | 3 cut(s) | 1402, 1690, 1723 |
| BseJI | GATNNNNATC | 1 cut(s) | 618 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 770, 790, 1046, 1486, 1681 |
| BseMI | GCAATG | 2 cut(s) | 930, 1078 |
| BseMII | CTCAG | 7 cut(s) | 28, 39, 107, 119, 198, 666, 1463 |
| BseNI | ACTGG | 2 cut(s) | 1439, 1592 |
| BseRI | GAGGAG | 2 cut(s) | 170, 1244 |
| BseSI | GKGCMC | 2 cut(s) | 1107, 1595 |
| BseXI | GCAGC | 2 cut(s) | 308, 336 |
| BsgI | GTGCAG | 1 cut(s) | 1247 |
| Bsh1236I | CGCG | 1 cut(s) | 441 |
| Bsh1285I | CGRYCG | 1 cut(s) | 9 |
| BshFI | GGCC | 3 cut(s) | 401, 1105, 1593 |
| BsiEI | CGRYCG | 1 cut(s) | 9 |
| BsiHKAI | GWGCWC | 1 cut(s) | 427 |
| BsiSI | CCGG | 3 cut(s) | 1040, 1350, 1577 |
| BslFI | GGGAC | 1 cut(s) | 1421 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 770, 790, 1046, 1486, 1681 |
| BsmAI | GTCTC | 1 cut(s) | 1533 |
| BsmBI | CGTCTC | 1 cut(s) | 1533 |
| BsmFI | GGGAC | 1 cut(s) | 1421 |
| BsmI | GAATGC | 1 cut(s) | 1073 |
| BsnI | GGCC | 3 cut(s) | 401, 1105, 1593 |
| Bsp120I | GGGCCC | 2 cut(s) | 1103, 1591 |
| Bsp1286I | GDGCHC | 3 cut(s) | 427, 1107, 1595 |
| Bsp13I | TCCGGA | 2 cut(s) | 1349, 1576 |
| Bsp1407I | TGTACA | 1 cut(s) | 692 |
| Bsp19I | CCATGG | 1 cut(s) | 1392 |
| Bsp68I | TCGCGA | 1 cut(s) | 441 |
| BspACI | CCGC | 3 cut(s) | 122, 656, 1180 |
| BspANI | GGCC | 3 cut(s) | 401, 1105, 1593 |
| BspCNI | CTCAG | 7 cut(s) | 27, 40, 106, 120, 199, 665, 1462 |
| BspEI | TCCGGA | 2 cut(s) | 1349, 1576 |
| BspFNI | CGCG | 1 cut(s) | 441 |
| BspLI | GGNNCC | 9 cut(s) | 318, 757, 803, 1105, 1471, 1491, 1592, 1593, 1647 |
| BspMAI | CTGCAG | 1 cut(s) | 1306 |
| BspMI | ACCTGC | 1 cut(s) | 1598 |
| BspPI | GGATC | 4 cut(s) | 614, 796, 809, 1471 |
| BspTI | CTTAAG | 1 cut(s) | 941 |
| BsrBI | CCGCTC | 1 cut(s) | 1180 |
| BsrDI | GCAATG | 2 cut(s) | 930, 1078 |
| BsrGI | TGTACA | 1 cut(s) | 692 |
| BsrI | ACTGG | 2 cut(s) | 1439, 1592 |
| BssECI | CCNNGG | 3 cut(s) | 1040, 1368, 1392 |
| BssSI | CACGAG | 1 cut(s) | 570 |
| BssT1I | CCWWGG | 2 cut(s) | 1368, 1392 |
| Bst2BI | CACGAG | 1 cut(s) | 570 |
| Bst4CI | ACNGT | 4 cut(s) | 755, 846, 1009, 1474 |
| Bst6I | CTCTTC | 2 cut(s) | 916, 1674 |
| BstAFI | CTTAAG | 1 cut(s) | 941 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 972 |
| BstAUI | TGTACA | 1 cut(s) | 692 |
| BstC8I | GCNNGC | 4 cut(s) | 63, 439, 814, 1561 |
| BstDSI | CCRYGG | 1 cut(s) | 1392 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1484 |
| BstF5I | GGATG | 3 cut(s) | 1402, 1690, 1723 |
| BstFNI | CGCG | 1 cut(s) | 441 |
| BstHHI | GCGC | 1 cut(s) | 1419 |
| BstMAI | GTCTC | 1 cut(s) | 1533 |
| BstMCI | CGRYCG | 1 cut(s) | 9 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 407, 972, 1511 |
| BstNSI | RCATGY | 3 cut(s) | 65, 164, 540 |
| BstSCI | CCNGG | 1 cut(s) | 1039 |
| BstSFI | CTRYAG | 1 cut(s) | 1302 |
| BstSLI | GKGCMC | 2 cut(s) | 1107, 1595 |
| BstUI | CGCG | 1 cut(s) | 441 |
| BstV1I | GCAGC | 2 cut(s) | 308, 336 |
| BstV2I | GAAGAC | 1 cut(s) | 634 |
| BstX2I | RGATCY | 1 cut(s) | 801 |
| BstYI | RGATCY | 1 cut(s) | 801 |
| BsuI | GTATCC | 1 cut(s) | 1194 |
| BsuRI | GGCC | 3 cut(s) | 401, 1105, 1593 |
| BtgI | CCRYGG | 1 cut(s) | 1392 |
| BtsCI | GGATG | 3 cut(s) | 1402, 1690, 1723 |
| BtsI | GCAGTG | 3 cut(s) | 717, 1030, 1311 |
| BtsIMutI | CAGTG | 8 cut(s) | 22, 717, 968, 1030, 1280, 1311, 1446, 1585 |
| BtuMI | TCGCGA | 1 cut(s) | 441 |
| BveI | ACCTGC | 1 cut(s) | 1598 |
| Cac8I | GCNNGC | 4 cut(s) | 63, 439, 814, 1561 |
| CaiI | CAGNNNCTG | 2 cut(s) | 701, 1655 |
| CfoI | GCGC | 1 cut(s) | 1419 |
| Cfr13I | GGNCC | 6 cut(s) | 1049, 1103, 1104, 1591, 1592, 1646 |
| CseI | GACGC | 3 cut(s) | 1515, 1661, 1694 |
| Csp6I | GTAC | 4 cut(s) | 693, 847, 1211, 1290 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 1060, 1081, 1095, 1116 |
| CviAII | CATG | 9 cut(s) | 62, 161, 321, 537, 827, 1127, 1358, 1393, 1676 |
| CviQI | GTAC | 4 cut(s) | 693, 847, 1211, 1290 |
| DraIII | CACNNNGTG | 1 cut(s) | 1441 |
| Eam1104I | CTCTTC | 2 cut(s) | 916, 1674 |
| EarI | CTCTTC | 2 cut(s) | 916, 1674 |
| Ecl136II | GAGCTC | 1 cut(s) | 425 |
| Eco130I | CCWWGG | 2 cut(s) | 1368, 1392 |
| Eco147I | AGGCCT | 1 cut(s) | 401 |
| Eco24I | GRGCYC | 3 cut(s) | 427, 1107, 1595 |
| Eco32I | GATATC | 3 cut(s) | 306, 1034, 1340 |
| Eco47I | GGWCC | 2 cut(s) | 1049, 1646 |
| Eco53kI | GAGCTC | 1 cut(s) | 425 |
| Eco57I | CTGAAG | 3 cut(s) | 398, 974, 1304 |
| EcoICRI | GAGCTC | 1 cut(s) | 425 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1484 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1591 |
| EcoRV | GATATC | 3 cut(s) | 306, 1034, 1340 |
| EcoT14I | CCWWGG | 2 cut(s) | 1368, 1392 |
| EcoT22I | ATGCAT | 1 cut(s) | 63 |
| EcoT38I | GRGCYC | 3 cut(s) | 427, 1107, 1595 |
| ErhI | CCWWGG | 2 cut(s) | 1368, 1392 |
| Esp3I | CGTCTC | 1 cut(s) | 1533 |
| FaeI | CATG | 9 cut(s) | 65, 164, 324, 540, 830, 1130, 1361, 1396, 1679 |
| FaqI | GGGAC | 1 cut(s) | 1421 |
| FatI | CATG | 9 cut(s) | 61, 160, 320, 536, 826, 1126, 1357, 1392, 1675 |
| FauNDI | CATATG | 2 cut(s) | 579, 1109 |
| FbaI | TGATCA | 2 cut(s) | 1129, 1326 |
| Fnu4HI | GCNGC | 2 cut(s) | 297, 325 |
| FokI | GGATG | 3 cut(s) | 1409, 1697, 1710 |
| FriOI | GRGCYC | 3 cut(s) | 427, 1107, 1595 |
| Fsp4HI | GCNGC | 2 cut(s) | 297, 325 |
| FspBI | CTAG | 3 cut(s) | 473, 809, 1515 |
| GlaI | GCGC | 1 cut(s) | 1418 |
| GluI | GCNGC | 2 cut(s) | 297, 325 |
| GsuI | CTGGAG | 1 cut(s) | 1440 |
| HaeIII | GGCC | 3 cut(s) | 401, 1105, 1593 |
| HapII | CCGG | 3 cut(s) | 1040, 1350, 1577 |
| HgaI | GACGC | 3 cut(s) | 1515, 1661, 1694 |
| HhaI | GCGC | 1 cut(s) | 1419 |
| Hin1II | CATG | 9 cut(s) | 65, 164, 324, 540, 830, 1130, 1361, 1396, 1679 |
| Hin6I | GCGC | 1 cut(s) | 1417 |
| HinP1I | GCGC | 1 cut(s) | 1417 |
| HincII | GTYRAC | 2 cut(s) | 100, 673 |
| HindII | GTYRAC | 2 cut(s) | 100, 673 |
| HindIII | AAGCTT | 2 cut(s) | 498, 1598 |
| HinfI | GANTC | 9 cut(s) | 52, 251, 551, 650, 823, 832, 977, 1258, 1432 |
| HpaI | GTTAAC | 1 cut(s) | 100 |
| HpaII | CCGG | 3 cut(s) | 1040, 1350, 1577 |
| HphI | GGTGA | 4 cut(s) | 577, 1427, 1438, 1612 |
| Hpy166II | GTNNAC | 5 cut(s) | 100, 673, 693, 1290, 1363 |
| Hpy188I | TCNGA | 9 cut(s) | 57, 129, 208, 256, 334, 873, 1159, 1228, 1384 |
| Hpy8I | GTNNAC | 5 cut(s) | 100, 673, 693, 1290, 1363 |
| HpyAV | CCTTC | 6 cut(s) | 32, 443, 996, 1168, 1546, 1577 |
| HpyCH4III | ACNGT | 4 cut(s) | 755, 846, 1009, 1474 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 407, 972, 1511 |
| Hsp92II | CATG | 9 cut(s) | 65, 164, 324, 540, 830, 1130, 1361, 1396, 1679 |
| HspAI | GCGC | 1 cut(s) | 1417 |
| Kpn2I | TCCGGA | 2 cut(s) | 1349, 1576 |
| Ksp22I | TGATCA | 2 cut(s) | 1129, 1326 |
| KspAI | GTTAAC | 1 cut(s) | 100 |
| LmnI | GCTCC | 4 cut(s) | 157, 1123, 1177, 1393 |
| Lsp1109I | GCAGC | 2 cut(s) | 308, 336 |
| LweI | GCATC | 5 cut(s) | 48, 205, 556, 1209, 1375 |
| MaeI | CTAG | 3 cut(s) | 473, 809, 1515 |
| MaeIII | GTNAC | 3 cut(s) | 349, 836, 1141 |
| MbiI | CCGCTC | 1 cut(s) | 1180 |
| MfeI | CAATTG | 1 cut(s) | 117 |
| MflI | RGATCY | 1 cut(s) | 801 |
| MhlI | GDGCHC | 3 cut(s) | 427, 1107, 1595 |
| MluCI | AATT | 8 cut(s) | 117, 223, 486, 599, 1088, 1221, 1507, 1735 |
| MlyI | GAGTC | 4 cut(s) | 260, 644, 832, 1267 |
| Mph1103I | ATGCAT | 1 cut(s) | 63 |
| MroI | TCCGGA | 2 cut(s) | 1349, 1576 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1088, 1494 |
| MseI | TTAA | 6 cut(s) | 33, 99, 419, 942, 1376, 1671 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1285 |
| MspA1I | CMGCKG | 1 cut(s) | 656 |
| MspCI | CTTAAG | 1 cut(s) | 941 |
| MspI | CCGG | 3 cut(s) | 1040, 1350, 1577 |
| MspR9I | CCNGG | 1 cut(s) | 1041 |
| MunI | CAATTG | 1 cut(s) | 117 |
| Mva1269I | GAATGC | 1 cut(s) | 1073 |
| MvnI | CGCG | 1 cut(s) | 441 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 407, 972, 1511 |
| NciI | CCSGG | 1 cut(s) | 1041 |
| NcoI | CCATGG | 1 cut(s) | 1392 |
| NdeI | CATATG | 2 cut(s) | 579, 1109 |
| NlaIII | CATG | 9 cut(s) | 65, 164, 324, 540, 830, 1130, 1361, 1396, 1679 |
| NlaIV | GGNNCC | 9 cut(s) | 318, 757, 803, 1105, 1471, 1491, 1592, 1593, 1647 |
| NmuCI | GTSAC | 1 cut(s) | 349 |
| NruI | TCGCGA | 1 cut(s) | 441 |
| NsiI | ATGCAT | 1 cut(s) | 63 |
| NspI | RCATGY | 3 cut(s) | 65, 164, 540 |
| OliI | CACNNNNGTG | 1 cut(s) | 1285 |
| PaeI | GCATGC | 1 cut(s) | 65 |
| PceI | AGGCCT | 1 cut(s) | 401 |
| PctI | GAATGC | 1 cut(s) | 1073 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1088, 1494 |
| PfeI | GAWTC | 5 cut(s) | 52, 551, 832, 977, 1432 |
| PflFI | GACNNNGTC | 1 cut(s) | 1655 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 790 |
| PkrI | GCNGC | 2 cut(s) | 298, 326 |
| PleI | GAGTC | 4 cut(s) | 259, 644, 831, 1266 |
| PpsI | GAGTC | 4 cut(s) | 259, 644, 831, 1266 |
| Psp124BI | GAGCTC | 1 cut(s) | 427 |
| PspN4I | GGNNCC | 9 cut(s) | 318, 757, 803, 1105, 1471, 1491, 1592, 1593, 1647 |
| PspOMI | GGGCCC | 2 cut(s) | 1103, 1591 |
| PspPI | GGNCC | 6 cut(s) | 1049, 1103, 1104, 1591, 1592, 1646 |
| PstI | CTGCAG | 1 cut(s) | 1306 |
| PstNI | CAGNNNCTG | 2 cut(s) | 701, 1655 |
| PsuI | RGATCY | 1 cut(s) | 801 |
| PsyI | GACNNNGTC | 1 cut(s) | 1655 |
| RruI | TCGCGA | 1 cut(s) | 441 |
| RsaI | GTAC | 4 cut(s) | 694, 848, 1212, 1291 |
| RsaNI | GTAC | 4 cut(s) | 693, 847, 1211, 1290 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1285 |
| SacI | GAGCTC | 1 cut(s) | 427 |
| SaqAI | TTAA | 6 cut(s) | 33, 99, 419, 942, 1376, 1671 |
| SatI | GCNGC | 2 cut(s) | 297, 325 |
| Sau96I | GGNCC | 6 cut(s) | 1049, 1103, 1104, 1591, 1592, 1646 |
| SchI | GAGTC | 4 cut(s) | 260, 644, 832, 1267 |
| ScrFI | CCNGG | 1 cut(s) | 1041 |
| SduI | GDGCHC | 3 cut(s) | 427, 1107, 1595 |
| SfaNI | GCATC | 5 cut(s) | 48, 205, 556, 1209, 1375 |
| SfcI | CTRYAG | 1 cut(s) | 1302 |
| SinI | GGWCC | 2 cut(s) | 1049, 1646 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1285 |
| SmlI | CTYRAG | 5 cut(s) | 309, 395, 941, 1062, 1255 |
| SmoI | CTYRAG | 5 cut(s) | 309, 395, 941, 1062, 1255 |
| SphI | GCATGC | 1 cut(s) | 65 |
| Sse9I | AATT | 8 cut(s) | 117, 223, 486, 599, 1088, 1221, 1507, 1735 |
| SseBI | AGGCCT | 1 cut(s) | 401 |
| SsiI | CCGC | 3 cut(s) | 122, 656, 1180 |
| SspI | AATATT | 1 cut(s) | 779 |
| SspMI | CTAG | 3 cut(s) | 473, 809, 1515 |
| SstI | GAGCTC | 1 cut(s) | 427 |
| StuI | AGGCCT | 1 cut(s) | 401 |
| StyD4I | CCNGG | 1 cut(s) | 1039 |
| StyI | CCWWGG | 2 cut(s) | 1368, 1392 |
| TaaI | ACNGT | 4 cut(s) | 755, 846, 1009, 1474 |
| TaqI | TCGA | 1 cut(s) | 889 |
| TaqII | GACCGA | 1 cut(s) | 23 |
| TasI | AATT | 8 cut(s) | 117, 223, 486, 599, 1088, 1221, 1507, 1735 |
| TatI | WGTACW | 3 cut(s) | 692, 1210, 1289 |
| TfiI | GAWTC | 5 cut(s) | 52, 551, 832, 977, 1432 |
| Tru1I | TTAA | 6 cut(s) | 33, 99, 419, 942, 1376, 1671 |
| Tru9I | TTAA | 6 cut(s) | 33, 99, 419, 942, 1376, 1671 |
| TscAI | CASTG | 8 cut(s) | 22, 717, 968, 1030, 1287, 1311, 1446, 1592 |
| TseFI | GTSAC | 1 cut(s) | 349 |
| TseI | GCWGC | 2 cut(s) | 296, 324 |
| Tsp45I | GTSAC | 1 cut(s) | 349 |
| TspDTI | ATGAA | 5 cut(s) | 282, 941, 974, 1187, 1692 |
| TspGWI | ACGGA | 3 cut(s) | 690, 890, 1304 |
| TspRI | CASTG | 8 cut(s) | 22, 717, 968, 1030, 1287, 1311, 1446, 1592 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1655 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 790 |
| Vha464I | CTTAAG | 1 cut(s) | 941 |
| VpaK11BI | GGWCC | 2 cut(s) | 1049, 1646 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1484 |
| XapI | RAATTY | 4 cut(s) | 223, 486, 599, 1088 |
| XbaI | TCTAGA | 1 cut(s) | 472 |
| XceI | RCATGY | 3 cut(s) | 65, 164, 540 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1088, 1494 |
| XspI | CTAG | 3 cut(s) | 473, 809, 1515 |
| Zsp2I | ATGCAT | 1 cut(s) | 63 |