MD17G1263300.v1.1

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004545
Physical Location & Seq
Forward (+)
32436789 .. 32440051
3263 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1263300.v1.1.491

Sequence Viewer

Length: 1488 bp
ATGGCTAAATCCTTCCAGGCAACAAGGATGAAGAGGAAAAGAAAATCTGCTGCTTGTGACACCCAAATCCAAATAGGCAACATGGATGATCAAATTGAAGAAAAGGGTCCCTTTATAACAGATCTTCCGAAGCCCGTCTTTTGGAATATTTTACTAAAGTTACCAACCAGGAGCATTCTCATTTGCAAATGTGTTTGCAAGACTTGGCGTGCTCGAATTTCGGACCCTGAATTTGCTAACCTGCACTTTGCACAAGCAGAGCCATGTCCTCTGCTGTCGCTTCTCGGTCCAACTCCAACACGTGTGCCAAGAACCCTTTACTTGGTTGAGCCTGAAGATCGCTCTGGGTTTGATTTGAAGGATTGTGCCTGTGCTGCTACTGGCAATGCTTGTGACCAGTCTCATGTCCACATGAAGCTTACCAAGTACAAGATCCCATTGCGCAACGATGAGGAGGTCATCAATAACAACAGTGATAACGGCAATGTGATGCCTAATGGGAACCGCGGCACCAAAAGGAAGACTTGCATCAGGATACCGCCACATCATCACAAGTACAATGTGGTGAATTCATGTAACGGCCTGCTTCTCTTGTCTGAGCCAATCCACAACGACCCTGCTGTTGTGTGCAATCCAGTAACCGGTGAATTTATAGACCTTCCTGAAAGCTCTAGGCCGCATCAGAATATAAGGATTCCTGTCGAATGTGGATTGGGTTTCAGTTCCGAGACTAATGAATATAAGGTGATACGAATTTTTGAAGCTGAAGCAGGCTATAGCTTTAAATTTGCTGAGATACACACACTTGGAACAGGTTCATGGAAGTCCATTGGTGAAGTTTCTGAAACAGCGGGTAACCTAGTATCCCCTACCTATGCGAAAGGAGTGCTTTATTGGTATGAGTCTTATGGCTATAGAATTCGTTCTTTTGATTTGAACACTGAAAAGTTCAAATCAGTTCCATCACCGCCTTTTCCGATCAAGCAATTTATGTATGTGGGCATGGGAGTTTTGGAAGGTTGTCTTTGCTTGTGTGATTCCTATGGTGATCTTATCACTGTGTGGACACTGACAGATTCTGGTGGAAAACATCGTTGGACGAAGGTGATTGCTATTGATAAAGATGATAGTGGAAGGTGGCCATATGGCGTATATACAGCTATGAAATACTATAAGAGCTATGGTTTGTTTATGTTTCATTCTCAAACAAATTCCTTCTTTTACTATCACCCAGAAAAACCAAGTTCATACGTTTATCTTAAGCTTTGTGGCTTCAAGGCAAATTTTCAAGCAATTAGTCATGTTCCGAGCTTTATTTTACTCAAGGACATTCTGGCAGGGAAGGATGTAGAAGTATTGAATATAAATACAAGGTGTGCAGGGTTGAAGCTGCAAGGAGAGACCAGAGCTCTATCTCTGAAGGAAGAAATTGCCGAATTCGAATCAGACCCTTTTTCGTCGGACAATTACTGTTTTAGCGACGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1 FBA_3

Protein Analysis

496

Amino Acids

55.89

Weight (kDa)

7.78

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 39 - 78 1.3e-07 F-box domain
F-box-like PF12937 39 - 77 1.7e-06 F-box-like
Beta-prop_KIB1-4 PF03478 176 - 380 1.8e-08 KIB1-4 beta-propeller
FBA_3 PF08268 177 - 413 3.5e-20 F-box associated beta propeller domain
FBA_1 PF07734 183 - 375 1.7e-17 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 116
Acc16I TGCGCA 1 cut(s) 443
Acc36I ACCTGC 1 cut(s) 249
AccB1I GGYRCC 1 cut(s) 509
AccII CGCG 1 cut(s) 507
AciI CCGC 6 cut(s) 505, 507, 539, 677, 851, 968
AclWI GGATC 1 cut(s) 427
AcoI YGGCCR 1 cut(s) 1139
AcuI CTGAAG 3 cut(s) 354, 786, 1439
AcvI CACGTG 1 cut(s) 302
AdeI CACNNNGTG 1 cut(s) 1062
AfaI GTAC 2 cut(s) 428, 557
AfiI CCNNNNNNNGG 3 cut(s) 141, 322, 641
AflII CTTAAG 1 cut(s) 1259
AflIII ACRYGT 2 cut(s) 299, 301
AgeI ACCGGT 1 cut(s) 641
AgsI TTSAA 9 cut(s) 98, 358, 761, 937, 952, 1276, 1289, 1360, 1387
AjnI CCWGG 2 cut(s) 15, 167
AjuI GAANNNNNNNTTGG 2 cut(s) 1078, 1110
Alw21I GWGCWC 2 cut(s) 214, 1411
Alw26I GTCTC 3 cut(s) 405, 722, 1394
AlwI GGATC 1 cut(s) 427
AlwNI CAGNNNCTG 1 cut(s) 1079
AoxI GGCC 3 cut(s) 580, 674, 1139
ApeKI GCWGC 3 cut(s) 50, 374, 1390
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
AsiGI ACCGGT 1 cut(s) 641
Asp700I GAANNNNTTC 2 cut(s) 814, 922
AspLEI GCGC 1 cut(s) 444
AspS9I GGNCC 3 cut(s) 107, 223, 287
AsuHPI GGTGA 8 cut(s) 577, 656, 757, 845, 957, 1058, 1117, 1220
AsuII TTCGAA 1 cut(s) 1440
AvaII GGWCC 3 cut(s) 107, 223, 287
BalI TGGCCA 1 cut(s) 1141
BanI GGYRCC 1 cut(s) 509
BanII GRGCYC 1 cut(s) 1411
BbrPI CACGTG 1 cut(s) 302
BbsI GAAGAC 1 cut(s) 527
Bbv12I GWGCWC 2 cut(s) 214, 1411
BbvI GCAGC 3 cut(s) 37, 361, 1377
BccI CCATC 1 cut(s) 970
BceAI ACGGC 2 cut(s) 496, 595
BcgI CGANNNNNNTGC 2 cut(s) 868, 902
BciT130I CCWGG 2 cut(s) 17, 169
BciVI GTATCC 2 cut(s) 528, 874
BclI TGATCA 1 cut(s) 88
BcoDI GTCTC 3 cut(s) 405, 722, 1394
BfaI CTAG 2 cut(s) 672, 860
BfmI CTRYAG 2 cut(s) 775, 913
BfrI CTTAAG 1 cut(s) 1259
BfuAI ACCTGC 1 cut(s) 249
BfuI GTATCC 2 cut(s) 528, 874
BglII AGATCT 1 cut(s) 121
BisI GCNGC 5 cut(s) 51, 375, 508, 677, 1391
BlsI GCNGC 5 cut(s) 52, 376, 509, 678, 1392
Bme1390I CCNGG 2 cut(s) 17, 169
Bme18I GGWCC 3 cut(s) 107, 223, 287
BmgT120I GGNCC 3 cut(s) 107, 223, 287
BmiI GGNNCC 5 cut(s) 108, 109, 225, 503, 511
BmrFI CCNGG 2 cut(s) 17, 169
BmsI GCATC 3 cut(s) 480, 537, 688
BpiI GAAGAC 1 cut(s) 527
Bpu14I TTCGAA 1 cut(s) 1440
BpuEI CTTGAG 1 cut(s) 1307
BsaAI YACGTR 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 1394
BsaJI CCNNGG 1 cut(s) 505
BsaWI WCCGGW 1 cut(s) 641
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 322, 641
Bse118I RCCGGY 1 cut(s) 641
Bse1I ACTGG 3 cut(s) 385, 397, 635
Bse3DI GCAATG 3 cut(s) 391, 437, 490
BseBI CCWGG 2 cut(s) 17, 169
BseDI CCNNGG 1 cut(s) 505
BseGI GGATG 3 cut(s) 33, 91, 1351
BseLI CCNNNNNNNGG 3 cut(s) 141, 322, 641
BseMI GCAATG 3 cut(s) 391, 437, 490
BseMII CTCAG 2 cut(s) 588, 783
BseNI ACTGG 3 cut(s) 385, 397, 635
BseRI GAGGAG 1 cut(s) 467
BseXI GCAGC 3 cut(s) 37, 361, 1377
BsgI GTGCAG 2 cut(s) 227, 1398
Bsh1236I CGCG 1 cut(s) 507
BshFI GGCC 3 cut(s) 582, 676, 1141
BshNI GGYRCC 1 cut(s) 509
BshTI ACCGGT 1 cut(s) 641
BsiHKAI GWGCWC 2 cut(s) 214, 1411
BsiSI CCGG 1 cut(s) 642
BslFI GGGAC 1 cut(s) 93
BslI CCNNNNNNNGG 3 cut(s) 141, 322, 641
BsmAI GTCTC 3 cut(s) 405, 722, 1394
BsmFI GGGAC 1 cut(s) 93
BsmI GAATGC 1 cut(s) 174
BsnI GGCC 3 cut(s) 582, 676, 1141
Bso31I GGTCTC 1 cut(s) 1394
Bsp119I TTCGAA 1 cut(s) 1440
Bsp1286I GDGCHC 2 cut(s) 214, 1411
Bsp143I GATC 6 cut(s) 88, 121, 337, 432, 978, 1048
BspACI CCGC 6 cut(s) 505, 507, 539, 677, 851, 968
BspANI GGCC 3 cut(s) 582, 676, 1141
BspCNI CTCAG 2 cut(s) 589, 784
BspFNI CGCG 1 cut(s) 507
BspLI GGNNCC 5 cut(s) 108, 109, 225, 503, 511
BspMI ACCTGC 1 cut(s) 249
BspPI GGATC 1 cut(s) 427
BspT104I TTCGAA 1 cut(s) 1440
BspT107I GGYRCC 1 cut(s) 509
BspTI CTTAAG 1 cut(s) 1259
BspTNI GGTCTC 1 cut(s) 1394
BsrDI GCAATG 3 cut(s) 391, 437, 490
BsrFI RCCGGY 1 cut(s) 641
BsrI ACTGG 3 cut(s) 385, 397, 635
BssAI RCCGGY 1 cut(s) 641
BssECI CCNNGG 1 cut(s) 505
BssMI GATC 6 cut(s) 88, 121, 337, 432, 978, 1048
Bst2UI CCWGG 2 cut(s) 17, 169
Bst4CI ACNGT 3 cut(s) 473, 1060, 1472
Bst6I CTCTTC 1 cut(s) 26
BstAFI CTTAAG 1 cut(s) 1259
BstBAI YACGTR 1 cut(s) 302
BstBI TTCGAA 1 cut(s) 1440
BstC8I GCNNGC 3 cut(s) 210, 584, 772
BstDEI CTNAG 2 cut(s) 597, 792
BstDSI CCRYGG 1 cut(s) 505
BstEII GGTNACC 1 cut(s) 854
BstF5I GGATG 3 cut(s) 33, 91, 1351
BstFNI CGCG 1 cut(s) 507
BstHHI GCGC 1 cut(s) 444
BstKTI GATC 6 cut(s) 91, 124, 340, 435, 981, 1051
BstMAI GTCTC 3 cut(s) 405, 722, 1394
BstMBI GATC 6 cut(s) 88, 121, 337, 432, 978, 1048
BstMWI GCNNNNNNNGC 1 cut(s) 374
BstNI CCWGG 2 cut(s) 17, 169
BstPI GGTNACC 1 cut(s) 854
BstSCI CCNGG 2 cut(s) 15, 167
BstSFI CTRYAG 2 cut(s) 775, 913
BstUI CGCG 1 cut(s) 507
BstV1I GCAGC 3 cut(s) 37, 361, 1377
BstV2I GAAGAC 1 cut(s) 527
BstX2I RGATCY 2 cut(s) 121, 432
BstYI RGATCY 2 cut(s) 121, 432
BsuI GTATCC 2 cut(s) 528, 874
BsuRI GGCC 3 cut(s) 582, 676, 1141
BtgI CCRYGG 1 cut(s) 505
BtsCI GGATG 3 cut(s) 33, 91, 1351
BtsIMutI CAGTG 4 cut(s) 478, 939, 1056, 1067
BveI ACCTGC 1 cut(s) 249
Cac8I GCNNGC 3 cut(s) 210, 584, 772
CaiI CAGNNNCTG 1 cut(s) 1079
CfoI GCGC 1 cut(s) 444
Cfr10I RCCGGY 1 cut(s) 641
Cfr13I GGNCC 3 cut(s) 107, 223, 287
Cfr42I CCGCGG 1 cut(s) 508
Csp6I GTAC 2 cut(s) 427, 556
CspAI ACCGGT 1 cut(s) 641
CviAII CATG 8 cut(s) 82, 264, 404, 412, 573, 819, 1003, 1301
CviQI GTAC 2 cut(s) 427, 556
DdeI CTNAG 2 cut(s) 597, 792
DpnI GATC 6 cut(s) 90, 123, 339, 434, 980, 1050
DpnII GATC 6 cut(s) 88, 121, 337, 432, 978, 1048
DraI TTTAAA 1 cut(s) 784
DraIII CACNNNGTG 1 cut(s) 1062
EaeI YGGCCR 1 cut(s) 1139
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 1409
Eco24I GRGCYC 1 cut(s) 1411
Eco31I GGTCTC 1 cut(s) 1394
Eco47I GGWCC 3 cut(s) 107, 223, 287
Eco53kI GAGCTC 1 cut(s) 1409
Eco57I CTGAAG 3 cut(s) 354, 786, 1439
Eco72I CACGTG 1 cut(s) 302
Eco91I GGTNACC 1 cut(s) 854
EcoICRI GAGCTC 1 cut(s) 1409
EcoO109I RGGNCCY 1 cut(s) 107
EcoO65I GGTNACC 1 cut(s) 854
EcoRI GAATTC 3 cut(s) 568, 918, 1436
EcoRII CCWGG 2 cut(s) 15, 167
EcoT38I GRGCYC 1 cut(s) 1411
FaeI CATG 8 cut(s) 85, 267, 407, 415, 576, 822, 1006, 1304
FaqI GGGAC 1 cut(s) 93
FatI CATG 8 cut(s) 81, 263, 403, 411, 572, 818, 1002, 1300
FauI CCCGC 1 cut(s) 844
FauNDI CATATG 1 cut(s) 1144
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 5 cut(s) 51, 375, 508, 677, 1391
FokI GGATG 3 cut(s) 40, 98, 1358
FriOI GRGCYC 1 cut(s) 1411
Fsp4HI GCNGC 5 cut(s) 51, 375, 508, 677, 1391
FspBI CTAG 2 cut(s) 672, 860
FspI TGCGCA 1 cut(s) 443
GlaI GCGC 1 cut(s) 443
GluI GCNGC 5 cut(s) 51, 375, 508, 677, 1391
HaeIII GGCC 3 cut(s) 582, 676, 1141
HapII CCGG 1 cut(s) 642
HhaI GCGC 1 cut(s) 444
Hin1II CATG 8 cut(s) 85, 267, 407, 415, 576, 822, 1006, 1304
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HindIII AAGCTT 2 cut(s) 416, 1262
HinfI GANTC 5 cut(s) 694, 902, 1037, 1076, 1442
HpaII CCGG 1 cut(s) 642
HphI GGTGA 8 cut(s) 577, 656, 757, 845, 957, 1058, 1117, 1220
Hpy166II GTNNAC 2 cut(s) 409, 1065
Hpy188III TCNNGA 2 cut(s) 532, 662
Hpy8I GTNNAC 2 cut(s) 409, 1065
Hpy99I CGWCG 2 cut(s) 1462, 1484
HpyAV CCTTC 9 cut(s) 22, 352, 668, 1010, 1096, 1128, 1225, 1336, 1414
HpyCH4III ACNGT 3 cut(s) 473, 1060, 1472
HpyCH4IV ACGT 2 cut(s) 301, 1251
HpyCH4V TGCA 8 cut(s) 186, 198, 244, 251, 528, 630, 1379, 1393
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
HpyF3I CTNAG 2 cut(s) 597, 792
HpySE526I ACGT 2 cut(s) 301, 1251
Hsp92II CATG 8 cut(s) 85, 267, 407, 415, 576, 822, 1006, 1304
HspAI GCGC 1 cut(s) 442
KflI GGGWCCC 1 cut(s) 107
Ksp22I TGATCA 1 cut(s) 88
KspI CCGCGG 1 cut(s) 508
Kzo9I GATC 6 cut(s) 88, 121, 337, 432, 978, 1048
LmnI GCTCC 1 cut(s) 171
Lsp1109I GCAGC 3 cut(s) 37, 361, 1377
LweI GCATC 3 cut(s) 480, 537, 688
MaeI CTAG 2 cut(s) 672, 860
MaeII ACGT 2 cut(s) 301, 1251
MaeIII GTNAC 6 cut(s) 56, 159, 392, 575, 637, 854
MalI GATC 6 cut(s) 90, 123, 339, 434, 980, 1050
MboI GATC 6 cut(s) 88, 121, 337, 432, 978, 1048
MboII GAAGA 6 cut(s) 43, 110, 116, 347, 532, 1436
MflI RGATCY 2 cut(s) 121, 432
MhlI GDGCHC 2 cut(s) 214, 1411
MlsI TGGCCA 1 cut(s) 1141
MluNI TGGCCA 1 cut(s) 1141
MlyI GAGTC 1 cut(s) 911
MmeI TCCRAC 4 cut(s) 314, 320, 1076, 1440
MnlI CCTC 4 cut(s) 27, 279, 445, 448
Mox20I TGGCCA 1 cut(s) 1141
MroXI GAANNNNTTC 2 cut(s) 814, 922
MscI TGGCCA 1 cut(s) 1141
MseI TTAA 2 cut(s) 783, 1260
Msp20I TGGCCA 1 cut(s) 1141
MspA1I CMGCKG 2 cut(s) 507, 851
MspCI CTTAAG 1 cut(s) 1259
MspI CCGG 1 cut(s) 642
MspR9I CCNGG 2 cut(s) 17, 169
Mva1269I GAATGC 1 cut(s) 174
MvaI CCWGG 2 cut(s) 17, 169
MvnI CGCG 1 cut(s) 507
MwoI GCNNNNNNNGC 1 cut(s) 374
NdeI CATATG 1 cut(s) 1144
NdeII GATC 6 cut(s) 88, 121, 337, 432, 978, 1048
NlaIII CATG 8 cut(s) 85, 267, 407, 415, 576, 822, 1006, 1304
NlaIV GGNNCC 5 cut(s) 108, 109, 225, 503, 511
NmuCI GTSAC 2 cut(s) 56, 392
NsbI TGCGCA 1 cut(s) 443
NspV TTCGAA 1 cut(s) 1440
PctI GAATGC 1 cut(s) 174
PdmI GAANNNNTTC 2 cut(s) 814, 922
PfeI GAWTC 4 cut(s) 694, 1037, 1076, 1442
PinAI ACCGGT 1 cut(s) 641
PkrI GCNGC 5 cut(s) 52, 376, 509, 678, 1392
PleI GAGTC 1 cut(s) 910
PmaCI CACGTG 1 cut(s) 302
PmlI CACGTG 1 cut(s) 302
PpsI GAGTC 1 cut(s) 910
Ppu21I YACGTR 1 cut(s) 302
PpuMI RGGWCCY 1 cut(s) 107
PsiI TTATAA 1 cut(s) 116
Psp124BI GAGCTC 1 cut(s) 1411
Psp5II RGGWCCY 1 cut(s) 107
Psp6I CCWGG 2 cut(s) 15, 167
PspCI CACGTG 1 cut(s) 302
PspEI GGTNACC 1 cut(s) 854
PspGI CCWGG 2 cut(s) 15, 167
PspN4I GGNNCC 5 cut(s) 108, 109, 225, 503, 511
PspPI GGNCC 3 cut(s) 107, 223, 287
PspPPI RGGWCCY 1 cut(s) 107
PstNI CAGNNNCTG 1 cut(s) 1079
PsuI RGATCY 2 cut(s) 121, 432
RsaI GTAC 2 cut(s) 428, 557
RsaNI GTAC 2 cut(s) 427, 556
SacI GAGCTC 1 cut(s) 1411
SacII CCGCGG 1 cut(s) 508
SaqAI TTAA 2 cut(s) 783, 1260
SatI GCNGC 5 cut(s) 51, 375, 508, 677, 1391
Sau3AI GATC 6 cut(s) 88, 121, 337, 432, 978, 1048
Sau96I GGNCC 3 cut(s) 107, 223, 287
SchI GAGTC 1 cut(s) 911
ScrFI CCNGG 2 cut(s) 17, 169
SduI GDGCHC 2 cut(s) 214, 1411
SfaNI GCATC 3 cut(s) 480, 537, 688
SfcI CTRYAG 2 cut(s) 775, 913
Sfr303I CCGCGG 1 cut(s) 508
SfuI TTCGAA 1 cut(s) 1440
SgrBI CCGCGG 1 cut(s) 508
SinI GGWCC 3 cut(s) 107, 223, 287
SmlI CTYRAG 2 cut(s) 1259, 1322
SmoI CTYRAG 2 cut(s) 1259, 1322
SsiI CCGC 6 cut(s) 505, 507, 539, 677, 851, 968
SspI AATATT 1 cut(s) 148
SspMI CTAG 2 cut(s) 672, 860
SstI GAGCTC 1 cut(s) 1411
StyD4I CCNGG 2 cut(s) 15, 167
TaaI ACNGT 3 cut(s) 473, 1060, 1472
TaiI ACGT 2 cut(s) 304, 1254
TaqI TCGA 3 cut(s) 214, 702, 1440
TaqII GACCGA 1 cut(s) 275
TatI WGTACW 2 cut(s) 426, 555
TauI GCSGC 2 cut(s) 510, 679
TfiI GAWTC 4 cut(s) 694, 1037, 1076, 1442
Tru1I TTAA 2 cut(s) 783, 1260
Tru9I TTAA 2 cut(s) 783, 1260
TscAI CASTG 4 cut(s) 478, 946, 1063, 1074
TseFI GTSAC 2 cut(s) 56, 392
TseI GCWGC 3 cut(s) 50, 374, 1390
Tsp45I GTSAC 2 cut(s) 56, 392
TspDTI ATGAA 8 cut(s) 44, 428, 561, 750, 807, 1178, 1187, 1236
TspRI CASTG 4 cut(s) 478, 946, 1063, 1074
Vha464I CTTAAG 1 cut(s) 1259
VpaK11BI GGWCC 3 cut(s) 107, 223, 287
XmnI GAANNNNTTC 2 cut(s) 814, 922
XspI CTAG 2 cut(s) 672, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.