Rh2BG355300

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004545
Physical Location & Seq
Reverse (-)
48758857 .. 48763219
4363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG355300.1

Sequence Viewer

Length: 1548 bp
ATGCTTAGCTCTCACAGTACTGGGAATGCTCTCTCTAAGGGAAATTGCATTCAGCCTCAACGTTGTGTTCCTAGGATAGCTATGAAAAGGAAAAGGAAGTCAATAGCTTATCCCACCAAAGTAGGCAACAAAGATGAACACATTGAACAAATGGGCCCTGTTATAACAGATCTTCCTAAGTTTATTTGTCAAATCATTCTACTAAAGCTCCCAACACGCAGCATTCTCATATGCAAATGTGTATGCAAGACTTGGCGTGTGCTAATTTCAGACCCGGAATTTGCCAAGATGCACTTTGCACAAGCAGAGGCCTATCCCATGGTCCGACCTTGGGGACCAACACGGGTGTCAAGAACCCTTTACTTGGTTGAACCTGAAGATGAATTCAGTTTGGCTATAAAGGACAAGGACTATTTGTCCGATTGTGACTATGGCTCATTGAAGTTTGACAAGTGTCATTTCCGCATGAATCTTGCCAAATATAAATTACCATTGCGGGATGCTGAGAAGGTAATGAATAATGATGATAATGTCAATCTGATGCGTAATAGGAAACATGGCTCTAAAAGGATGCCTTGCATCAAGATTAGGCCCAACGATCACAAATACAATGTGGTGAATTCGTGCAACGGCTTCCTTTGTTTGTCTGAACCAATTTTTAATGACCCAGTTGTTGTCTGCAACCCTGTAACAGGAGAGTTTATGCACCTTCCTGAAGCTTCTAAGCGGGAGAACCAAAAGGTTACCATCCATTGCGGAATTGGTTTCAGTCCAAAAGCTAACAGATATAAGGTGGTGCGAATCTTTGATCAAGGGTCACCTCATCCTATTACGATGGCTGAGGCACACACTCTTGGGTCAAGTTCATGGAAGCTCATTGGTAGTTGTCCCTGCTCATCATATAACCAGCTAGCGTTCCCCACCTATATTGAAGGAGCACTTTATTGGTTTTACTATGATCCTTTAAAATATGTCATACTTTCATTTGACTTGGACAGTGAGAACTTCCAATCACTTCCACCACTGCCTTTTCAACGAGAGCAATATCGTAATGTGAGCATGGGAGTTTTGGGAAATTGCCTTTGTGTCTGTGATGCTCATGGTATTCATATCAATTTGTGGGTACTGAAAAATCATGGTGCAAAAAGATCTTGGAGGAGGAAGATTTCTATTGAAACAGATCATTTTGGGTTTGATGGTGAAAGGTGGCCTTCTGGCTTATATAAACCAATGAAATACTGTAAGGATGGGGGCTTGTTGATGTTTAACTATCGTACTAACGCGTTGATTTACCATCATCCAAAAACCCACAGATTTATTTATCTCAAGCTTCGTGGCATCAAGTCAAATTTTTCAGCTATCAGTCATGTTCCAAGCTTTATTTCACTCAAGGACATTTTGGTGGGGGATGATGTCAACATACTGAATATAAATTCGAGGTGTGCACAGATGAAGCTGCTAGGAGAGACAAGAGCTCTTTTTCTGGAGGAAGAAATTGCAGATTTAGCATCAGACTTTTTCACGTCTGATAGTAGCGATGAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

515

Amino Acids

58.97

Weight (kDa)

8.94

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 55 - 94 1.1e-06 F-box domain
F-box-like PF12937 55 - 94 2.2e-06 F-box-like
FBA_1 PF07734 196 - 392 7.5e-24 F-box associated beta propeller domain
FBA_3 PF08268 198 - 393 1.4e-21 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 201 - 386 3.3e-07 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 164
AccII CGCG 1 cut(s) 1283
AciI CCGC 4 cut(s) 463, 496, 727, 756
AclI AACGTT 1 cut(s) 61
AclWI GGATC 1 cut(s) 953
AcsI RAATTY 5 cut(s) 278, 383, 619, 1348, 1432
AcuI CTGAAG 2 cut(s) 396, 735
AfaI GTAC 3 cut(s) 19, 1125, 1276
AfiI CCNNNNNNNGG 3 cut(s) 331, 364, 692
AflIII ACRYGT 1 cut(s) 1281
AgsI TTSAA 6 cut(s) 146, 371, 442, 932, 1034, 1175
AhdI GACNNNNNGTC 1 cut(s) 415
AjiI CACGTC 1 cut(s) 1524
AjuI GAANNNNNNNTTGG 2 cut(s) 1366, 1398
Alw21I GWGCWC 3 cut(s) 940, 1447, 1477
Alw26I GTCTC 1 cut(s) 1460
Alw44I GTGCAC 1 cut(s) 1443
AlwI GGATC 1 cut(s) 953
AoxI GGCC 4 cut(s) 154, 309, 590, 1208
ApaI GGGCCC 1 cut(s) 158
ApaLI GTGCAC 1 cut(s) 1443
ApeKI GCWGC 2 cut(s) 219, 1456
ApoI RAATTY 5 cut(s) 278, 383, 619, 1348, 1432
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
AspA2I CCTAGG 1 cut(s) 71
AspS9I GGNCC 5 cut(s) 154, 155, 322, 335, 591
AsuC2I CCSGG 1 cut(s) 275
AsuHPI GGTGA 3 cut(s) 628, 810, 1211
AsuNHI GCTAGC 1 cut(s) 910
AvaII GGWCC 2 cut(s) 322, 335
AvrII CCTAGG 1 cut(s) 71
BaeGI GKGCMC 2 cut(s) 158, 1447
BanII GRGCYC 2 cut(s) 158, 1477
Bbv12I GWGCWC 3 cut(s) 940, 1447, 1477
BbvCI CCTCAGC 1 cut(s) 840
BbvI GCAGC 2 cut(s) 231, 1443
BccI CCATC 5 cut(s) 755, 829, 1190, 1241, 1302
BceAI ACGGC 1 cut(s) 646
BclI TGATCA 1 cut(s) 808
BcnI CCSGG 1 cut(s) 275
BcoDI GTCTC 1 cut(s) 1460
BfaI CTAG 3 cut(s) 72, 911, 1460
BglII AGATCT 2 cut(s) 169, 1148
BisI GCNGC 2 cut(s) 220, 1457
BlnI CCTAGG 1 cut(s) 71
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 2 cut(s) 221, 1458
BmcAI AGTACT 1 cut(s) 19
Bme1390I CCNGG 1 cut(s) 275
Bme18I GGWCC 2 cut(s) 322, 335
BmeRI GACNNNNNGTC 1 cut(s) 415
BmgBI CACGTC 1 cut(s) 1524
BmgT120I GGNCC 5 cut(s) 154, 155, 322, 335, 591
BmiI GGNNCC 2 cut(s) 156, 336
BmrFI CCNGG 1 cut(s) 275
BmrI ACTGGG 2 cut(s) 30, 662
BmsI GCATC 8 cut(s) 279, 490, 531, 561, 588, 1084, 1347, 1517
BmtI GCTAGC 1 cut(s) 914
BmuI ACTGGG 2 cut(s) 30, 662
BoxI GACNNNNGTC 1 cut(s) 453
BpmI CTGGAG 1 cut(s) 1505
Bpu10I CCTNAGC 1 cut(s) 840
Bpu1102I GCTNAGC 1 cut(s) 5
BpuEI CTTGAG 2 cut(s) 1310, 1373
BpuMI CCSGG 1 cut(s) 275
BsaJI CCNNGG 3 cut(s) 71, 318, 329
BsaXI ACNNNNNCTCC 2 cut(s) 192, 222
Bsc4I CCNNNNNNNGG 3 cut(s) 331, 364, 692
Bse1I ACTGG 2 cut(s) 25, 668
Bse3DI GCAATG 2 cut(s) 491, 751
BseDI CCNNGG 3 cut(s) 71, 318, 329
BseGI GGATG 7 cut(s) 505, 576, 747, 823, 1252, 1297, 1414
BseLI CCNNNNNNNGG 3 cut(s) 331, 364, 692
BseMI GCAATG 2 cut(s) 491, 751
BseMII CTCAG 2 cut(s) 495, 831
BseNI ACTGG 2 cut(s) 25, 668
BseRI GAGGAG 1 cut(s) 1171
BseSI GKGCMC 2 cut(s) 158, 1447
BseXI GCAGC 2 cut(s) 231, 1443
Bsh1236I CGCG 1 cut(s) 1283
BshFI GGCC 4 cut(s) 156, 311, 592, 1210
BsiHKAI GWGCWC 3 cut(s) 940, 1447, 1477
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 2 cut(s) 348, 873
BslI CCNNNNNNNGG 3 cut(s) 331, 364, 692
BsmAI GTCTC 1 cut(s) 1460
BsmFI GGGAC 2 cut(s) 348, 873
BsmI GAATGC 3 cut(s) 31, 48, 222
BsnI GGCC 4 cut(s) 156, 311, 592, 1210
Bsp120I GGGCCC 1 cut(s) 154
Bsp1286I GDGCHC 4 cut(s) 158, 940, 1447, 1477
Bsp143I GATC 6 cut(s) 169, 598, 808, 958, 1148, 1180
Bsp1720I GCTNAGC 1 cut(s) 5
Bsp19I CCATGG 1 cut(s) 318
BspACI CCGC 4 cut(s) 463, 496, 727, 756
BspANI GGCC 4 cut(s) 156, 311, 592, 1210
BspCNI CTCAG 2 cut(s) 496, 832
BspFNI CGCG 1 cut(s) 1283
BspLI GGNNCC 2 cut(s) 156, 336
BspOI GCTAGC 1 cut(s) 914
BspPI GGATC 1 cut(s) 953
BsrDI GCAATG 2 cut(s) 491, 751
BsrI ACTGG 2 cut(s) 25, 668
BssECI CCNNGG 3 cut(s) 71, 318, 329
BssMI GATC 6 cut(s) 169, 598, 808, 958, 1148, 1180
BssT1I CCWWGG 3 cut(s) 71, 318, 329
Bst4CI ACNGT 3 cut(s) 17, 998, 1241
BstC8I GCNNGC 1 cut(s) 912
BstDEI CTNAG 6 cut(s) 5, 36, 177, 504, 723, 840
BstDSI CCRYGG 1 cut(s) 318
BstEII GGTNACC 2 cut(s) 742, 816
BstENI CCTNNNNNAGG 1 cut(s) 690
BstF5I GGATG 7 cut(s) 505, 576, 747, 823, 1252, 1297, 1414
BstFNI CGCG 1 cut(s) 1283
BstKTI GATC 6 cut(s) 172, 601, 811, 961, 1151, 1183
BstMAI GTCTC 1 cut(s) 1460
BstMBI GATC 6 cut(s) 169, 598, 808, 958, 1148, 1180
BstMWI GCNNNNNNNGC 1 cut(s) 1505
BstPAI GACNNNNGTC 1 cut(s) 453
BstPI GGTNACC 2 cut(s) 742, 816
BstSCI CCNGG 1 cut(s) 273
BstSLI GKGCMC 2 cut(s) 158, 1447
BstUI CGCG 1 cut(s) 1283
BstV1I GCAGC 2 cut(s) 231, 1443
BstX2I RGATCY 2 cut(s) 169, 1148
BstYI RGATCY 2 cut(s) 169, 1148
BsuRI GGCC 4 cut(s) 156, 311, 592, 1210
BtgI CCRYGG 1 cut(s) 318
BtrI CACGTC 1 cut(s) 1524
BtsCI GGATG 7 cut(s) 505, 576, 747, 823, 1252, 1297, 1414
BtsI GCAGTG 1 cut(s) 1022
BtsIMutI CAGTG 2 cut(s) 1003, 1022
Cac8I GCNNGC 1 cut(s) 912
Cfr13I GGNCC 5 cut(s) 154, 155, 322, 335, 591
Csp6I GTAC 3 cut(s) 18, 1124, 1275
CspCI CAANNNNNGTGG 4 cut(s) 910, 945, 1315, 1350
CviAII CATG 8 cut(s) 319, 466, 557, 867, 1060, 1100, 1136, 1367
CviQI GTAC 3 cut(s) 18, 1124, 1275
DdeI CTNAG 6 cut(s) 5, 36, 177, 504, 723, 840
DpnI GATC 6 cut(s) 171, 600, 810, 960, 1150, 1182
DpnII GATC 6 cut(s) 169, 598, 808, 958, 1148, 1180
DraI TTTAAA 1 cut(s) 966
DriI GACNNNNNGTC 1 cut(s) 415
Eam1105I GACNNNNNGTC 1 cut(s) 415
Ecl136II GAGCTC 1 cut(s) 1475
Eco130I CCWWGG 3 cut(s) 71, 318, 329
Eco147I AGGCCT 1 cut(s) 311
Eco24I GRGCYC 2 cut(s) 158, 1477
Eco47I GGWCC 2 cut(s) 322, 335
Eco53kI GAGCTC 1 cut(s) 1475
Eco57I CTGAAG 2 cut(s) 396, 735
Eco91I GGTNACC 2 cut(s) 742, 816
EcoICRI GAGCTC 1 cut(s) 1475
EcoNI CCTNNNNNAGG 1 cut(s) 690
EcoO109I RGGNCCY 1 cut(s) 155
EcoO65I GGTNACC 2 cut(s) 742, 816
EcoRI GAATTC 2 cut(s) 383, 619
EcoT14I CCWWGG 3 cut(s) 71, 318, 329
EcoT38I GRGCYC 2 cut(s) 158, 1477
ErhI CCWWGG 3 cut(s) 71, 318, 329
FaeI CATG 8 cut(s) 322, 469, 560, 870, 1063, 1103, 1139, 1370
FalI AAGNNNNNCTT 8 cut(s) 278, 310, 559, 591, 924, 956, 1195, 1227
FaqI GGGAC 2 cut(s) 348, 873
FatI CATG 8 cut(s) 318, 465, 556, 866, 1059, 1099, 1135, 1366
FauI CCCGC 2 cut(s) 489, 720
FauNDI CATATG 1 cut(s) 230
FbaI TGATCA 1 cut(s) 808
Fnu4HI GCNGC 2 cut(s) 220, 1457
FokI GGATG 7 cut(s) 512, 583, 734, 810, 1259, 1284, 1421
FriOI GRGCYC 2 cut(s) 158, 1477
Fsp4HI GCNGC 2 cut(s) 220, 1457
FspBI CTAG 3 cut(s) 72, 911, 1460
GluI GCNGC 2 cut(s) 220, 1457
GsuI CTGGAG 1 cut(s) 1505
HaeIII GGCC 4 cut(s) 156, 311, 592, 1210
HapII CCGG 1 cut(s) 275
Hin1II CATG 8 cut(s) 322, 469, 560, 870, 1063, 1103, 1139, 1370
HincII GTYRAC 1 cut(s) 1417
HindII GTYRAC 1 cut(s) 1417
HindIII AAGCTT 3 cut(s) 717, 1328, 1375
HinfI GANTC 2 cut(s) 469, 801
HpaII CCGG 1 cut(s) 275
HphI GGTGA 3 cut(s) 628, 810, 1211
Hpy166II GTNNAC 2 cut(s) 1417, 1445
Hpy188I TCNGA 7 cut(s) 271, 326, 421, 540, 649, 1513, 1528
Hpy188III TCNNGA 4 cut(s) 351, 583, 713, 1484
Hpy8I GTNNAC 2 cut(s) 1417, 1445
HpyAV CCTTC 4 cut(s) 502, 719, 926, 1221
HpyCH4III ACNGT 3 cut(s) 17, 998, 1241
HpyCH4IV ACGT 2 cut(s) 61, 1523
HpyF10VI GCNNNNNNNGC 1 cut(s) 1505
HpyF3I CTNAG 6 cut(s) 5, 36, 177, 504, 723, 840
HpySE526I ACGT 2 cut(s) 61, 1523
Hsp92II CATG 8 cut(s) 322, 469, 560, 870, 1063, 1103, 1139, 1370
Ksp22I TGATCA 1 cut(s) 808
Kzo9I GATC 6 cut(s) 169, 598, 808, 958, 1148, 1180
LmnI GCTCC 2 cut(s) 213, 935
Lsp1109I GCAGC 2 cut(s) 231, 1443
LweI GCATC 8 cut(s) 279, 490, 531, 561, 588, 1084, 1347, 1517
MaeI CTAG 3 cut(s) 72, 911, 1460
MaeII ACGT 2 cut(s) 61, 1523
MaeIII GTNAC 4 cut(s) 425, 688, 742, 816
MalI GATC 6 cut(s) 171, 600, 810, 960, 1150, 1182
MboI GATC 6 cut(s) 169, 598, 808, 958, 1148, 1180
MboII GAAGA 4 cut(s) 164, 389, 1174, 1502
MflI RGATCY 2 cut(s) 169, 1148
MhlI GDGCHC 4 cut(s) 158, 940, 1447, 1477
MluI ACGCGT 1 cut(s) 1281
MmeI TCCRAC 1 cut(s) 349
MnlI CCTC 8 cut(s) 66, 301, 831, 835, 1149, 1152, 1431, 1480
MseI TTAA 3 cut(s) 660, 965, 1266
MslI CAYNNNNRTG 1 cut(s) 1400
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 1 cut(s) 275
Mva1269I GAATGC 3 cut(s) 31, 48, 222
MvnI CGCG 1 cut(s) 1283
MwoI GCNNNNNNNGC 1 cut(s) 1505
NciI CCSGG 1 cut(s) 275
NcoI CCATGG 1 cut(s) 318
NdeI CATATG 1 cut(s) 230
NdeII GATC 6 cut(s) 169, 598, 808, 958, 1148, 1180
NheI GCTAGC 1 cut(s) 910
NlaIII CATG 8 cut(s) 322, 469, 560, 870, 1063, 1103, 1139, 1370
NlaIV GGNNCC 2 cut(s) 156, 336
NmuCI GTSAC 2 cut(s) 425, 816
PceI AGGCCT 1 cut(s) 311
PctI GAATGC 3 cut(s) 31, 48, 222
PfeI GAWTC 2 cut(s) 469, 801
PkrI GCNGC 2 cut(s) 221, 1458
PshAI GACNNNNGTC 1 cut(s) 453
PsiI TTATAA 1 cut(s) 164
Psp124BI GAGCTC 1 cut(s) 1477
Psp1406I AACGTT 1 cut(s) 61
PspEI GGTNACC 2 cut(s) 742, 816
PspN4I GGNNCC 2 cut(s) 156, 336
PspOMI GGGCCC 1 cut(s) 154
PspPI GGNCC 5 cut(s) 154, 155, 322, 335, 591
PsuI RGATCY 2 cut(s) 169, 1148
RsaI GTAC 3 cut(s) 19, 1125, 1276
RsaNI GTAC 3 cut(s) 18, 1124, 1275
RseI CAYNNNNRTG 1 cut(s) 1400
SacI GAGCTC 1 cut(s) 1477
SaqAI TTAA 3 cut(s) 660, 965, 1266
SatI GCNGC 2 cut(s) 220, 1457
Sau3AI GATC 6 cut(s) 169, 598, 808, 958, 1148, 1180
Sau96I GGNCC 5 cut(s) 154, 155, 322, 335, 591
ScaI AGTACT 1 cut(s) 19
ScrFI CCNGG 1 cut(s) 275
SduI GDGCHC 4 cut(s) 158, 940, 1447, 1477
SfaNI GCATC 8 cut(s) 279, 490, 531, 561, 588, 1084, 1347, 1517
SinI GGWCC 2 cut(s) 322, 335
SmiMI CAYNNNNRTG 1 cut(s) 1400
SmlI CTYRAG 2 cut(s) 1325, 1388
SmoI CTYRAG 2 cut(s) 1325, 1388
SseBI AGGCCT 1 cut(s) 311
SsiI CCGC 4 cut(s) 463, 496, 727, 756
SspMI CTAG 3 cut(s) 72, 911, 1460
SstI GAGCTC 1 cut(s) 1477
StuI AGGCCT 1 cut(s) 311
StyD4I CCNGG 1 cut(s) 273
StyI CCWWGG 3 cut(s) 71, 318, 329
TaaI ACNGT 3 cut(s) 17, 998, 1241
TaiI ACGT 2 cut(s) 64, 1526
TaqI TCGA 1 cut(s) 1436
TatI WGTACW 1 cut(s) 17
TfiI GAWTC 2 cut(s) 469, 801
Tru1I TTAA 3 cut(s) 660, 965, 1266
Tru9I TTAA 3 cut(s) 660, 965, 1266
TscAI CASTG 2 cut(s) 1003, 1029
TseFI GTSAC 2 cut(s) 425, 816
TseI GCWGC 2 cut(s) 219, 1456
Tsp45I GTSAC 2 cut(s) 425, 816
TspRI CASTG 2 cut(s) 1003, 1029
VneI GTGCAC 1 cut(s) 1443
VpaK11BI GGWCC 2 cut(s) 322, 335
XagI CCTNNNNNAGG 1 cut(s) 690
XapI RAATTY 5 cut(s) 278, 383, 619, 1348, 1432
XcmI CCANNNNNNNNNTGG 1 cut(s) 758
XmaJI CCTAGG 1 cut(s) 71
XspI CTAG 3 cut(s) 72, 911, 1460
ZrmI AGTACT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.