Rh2DG373000

F-box associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004545
Physical Location & Seq
Reverse (-)
53078749 .. 53081827
3079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG373000.1

Sequence Viewer

Length: 1362 bp
ATGAAAAGGAAAAGGAAGCCAATAGCTTATCCCACCAAAGTAGGCAACAAAGATGAACACATTGAACAAATGGGCCCTGTTATAACAGATCTTCCTAAGTTTATTTGTCAAATCATTCTACTAAAGCTCCCAACACGCAGCATTCTCATATGCAAATGTGTATGCAAGACTTGGCGTGTGCTAATTTCTGACCCGGAATTTGCCAAGATGCACTTTGCACAAGCAGAGGCCTATCCCATGGTCCGACCTTGGGGACCAACACGGGTGTCAAGAACCCTTTACTTGGTTGAACCTGAAGATGAATTCAGTTTGGCTATAAAGGACAAGGACTATTTGTCCGATTGTGACTATGGCTCATTGAAGTTTGACAAGTGTCATTTCCGCATGAATCTTGCCAAATATAAATTACCATTGCGGGATGCTGAGAAGGTAATGAATAATGATGATAATGTCAATCTGATGCGTAATAGGAAACATGGCACTAAAAGGATGCCTTGCATCAAGATTAGGCCCAACGATCACAAATACAATGTGGTGAATTCGTGCAACAGCTTCCTTTGTTTGTCTGAACCAATTTTTAATGACCCAGTTGTTGTCTGCAACCCTGTAACAGGAGAGTTTATGCACCTTCCTGAAGCTTCTAAGCGGGAGAACCAAAAGGTTACCATCCATTGCGGAATTGGTTTCAGTCCAAAAGCTAACAGATATAAGGTGGTGGGAATCTTTGATCAAGGGTCACCTCATCCTATTACGATGGCTGAGGTACACACTCTTGGGTCAAGTTCATGGAAGCTCATTGGTAGTTGTCCGTGCTCATCATATAACCAGCTAGCGTTCCCCACCTATATTGAAGGAGCACTTTATTGGTTTTACTATGATCCTTTAAAATACGTCATACTTTCATTTGACTTGGACAGTGAGAACTTCCAATCACTTCCACCACTGCCTTTTCAACGAGAGCAATATCGTAATGTGAGCATGGGAGTTTTGGGAAATTGTCTTTGTGTCTGTGATGCTCATGGTATTCATATCAATTTGTGGGTACTGAAAAATCATGGTGCAAAAAGATCTTGGAGGAGGAAGATTTCTATTGAAACAGATCATTTTGGGTTTGATGGTGAAAGGTGGCCTTCTGGCTTATATAAACCAATGAAATACTGTAAGGATGGGGGCTTGTTGATGTTTAACTATCGTACTAACACGTTGATTTACCATCATCCAAAAACCCACAGATTTATTTATCTCAAGCTTCGTGGCATCAAGTCAAATTTTTCAGCTATCAGTCATGTTCCAAGCTTTATTTCACTCAAGGACATTTTGGTGGGGGATGATGTCAACATACTGAATATAAATTCAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

453

Amino Acids

52.26

Weight (kDa)

9.19

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 28 - 67 9.6e-07 F-box domain
F-box-like PF12937 28 - 67 1.8e-06 F-box-like
FBA_1 PF07734 167 - 365 6.8e-23 F-box associated beta propeller domain
FBA_3 PF08268 172 - 366 1.3e-21 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 174 - 359 3.9e-07 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 83
AciI CCGC 4 cut(s) 382, 415, 646, 675
AclWI GGATC 1 cut(s) 872
AcsI RAATTY 5 cut(s) 197, 302, 538, 1267, 1351
AcuI CTGAAG 2 cut(s) 315, 654
AfaI GTAC 3 cut(s) 765, 1044, 1195
AfiI CCNNNNNNNGG 3 cut(s) 250, 283, 611
AflIII ACRYGT 1 cut(s) 1200
AgsI TTSAA 7 cut(s) 65, 290, 361, 851, 953, 1094, 1356
AhdI GACNNNNNGTC 1 cut(s) 334
AjuI GAANNNNNNNTTGG 2 cut(s) 1285, 1317
Alw21I GWGCWC 2 cut(s) 815, 859
AlwI GGATC 1 cut(s) 872
AoxI GGCC 4 cut(s) 73, 228, 509, 1127
ApaI GGGCCC 1 cut(s) 77
ApeKI GCWGC 1 cut(s) 138
ApoI RAATTY 5 cut(s) 197, 302, 538, 1267, 1351
ArsI GACNNNNNNTTYG 2 cut(s) 182, 214
AspS9I GGNCC 5 cut(s) 73, 74, 241, 254, 510
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 3 cut(s) 547, 729, 1130
AsuNHI GCTAGC 1 cut(s) 829
AvaII GGWCC 2 cut(s) 241, 254
BaeGI GKGCMC 1 cut(s) 77
BanII GRGCYC 1 cut(s) 77
Bbv12I GWGCWC 2 cut(s) 815, 859
BbvCI CCTCAGC 1 cut(s) 759
BbvI GCAGC 1 cut(s) 150
BccI CCATC 5 cut(s) 674, 748, 1109, 1160, 1221
BclI TGATCA 1 cut(s) 727
BcnI CCSGG 1 cut(s) 194
BfaI CTAG 1 cut(s) 830
BglII AGATCT 2 cut(s) 88, 1067
BisI GCNGC 1 cut(s) 139
BlsI GCNGC 1 cut(s) 140
Bme1390I CCNGG 1 cut(s) 194
Bme18I GGWCC 2 cut(s) 241, 254
BmeRI GACNNNNNGTC 1 cut(s) 334
BmgT120I GGNCC 5 cut(s) 73, 74, 241, 254, 510
BmiI GGNNCC 2 cut(s) 75, 255
BmrFI CCNGG 1 cut(s) 194
BmrI ACTGGG 1 cut(s) 581
BmsI GCATC 7 cut(s) 198, 409, 450, 480, 507, 1003, 1266
BmtI GCTAGC 1 cut(s) 833
BmuI ACTGGG 1 cut(s) 581
BoxI GACNNNNGTC 1 cut(s) 372
Bpu10I CCTNAGC 1 cut(s) 759
BpuEI CTTGAG 2 cut(s) 1229, 1292
BpuMI CCSGG 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 237, 248
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 3 cut(s) 250, 283, 611
Bse1I ACTGG 1 cut(s) 587
Bse3DI GCAATG 2 cut(s) 410, 670
BseDI CCNNGG 2 cut(s) 237, 248
BseGI GGATG 7 cut(s) 424, 495, 666, 742, 1171, 1216, 1333
BseLI CCNNNNNNNGG 3 cut(s) 250, 283, 611
BseMI GCAATG 2 cut(s) 410, 670
BseMII CTCAG 2 cut(s) 414, 750
BseNI ACTGG 1 cut(s) 587
BseRI GAGGAG 1 cut(s) 1090
BseSI GKGCMC 1 cut(s) 77
BseXI GCAGC 1 cut(s) 150
BshFI GGCC 4 cut(s) 75, 230, 511, 1129
BsiHKAI GWGCWC 2 cut(s) 815, 859
BsiSI CCGG 1 cut(s) 194
BslFI GGGAC 1 cut(s) 267
BslI CCNNNNNNNGG 3 cut(s) 250, 283, 611
BsmFI GGGAC 1 cut(s) 267
BsmI GAATGC 1 cut(s) 141
BsnI GGCC 4 cut(s) 75, 230, 511, 1129
Bsp120I GGGCCC 1 cut(s) 73
Bsp1286I GDGCHC 3 cut(s) 77, 815, 859
Bsp143I GATC 6 cut(s) 88, 517, 727, 877, 1067, 1099
Bsp19I CCATGG 1 cut(s) 237
BspACI CCGC 4 cut(s) 382, 415, 646, 675
BspANI GGCC 4 cut(s) 75, 230, 511, 1129
BspCNI CTCAG 2 cut(s) 415, 751
BspLI GGNNCC 2 cut(s) 75, 255
BspOI GCTAGC 1 cut(s) 833
BspPI GGATC 1 cut(s) 872
BsrDI GCAATG 2 cut(s) 410, 670
BsrI ACTGG 1 cut(s) 587
BssECI CCNNGG 2 cut(s) 237, 248
BssMI GATC 6 cut(s) 88, 517, 727, 877, 1067, 1099
BssT1I CCWWGG 2 cut(s) 237, 248
Bst4CI ACNGT 2 cut(s) 917, 1160
BstC8I GCNNGC 1 cut(s) 831
BstDEI CTNAG 4 cut(s) 96, 423, 642, 759
BstDSI CCRYGG 1 cut(s) 237
BstEII GGTNACC 2 cut(s) 661, 735
BstENI CCTNNNNNAGG 1 cut(s) 609
BstF5I GGATG 7 cut(s) 424, 495, 666, 742, 1171, 1216, 1333
BstKTI GATC 6 cut(s) 91, 520, 730, 880, 1070, 1102
BstMBI GATC 6 cut(s) 88, 517, 727, 877, 1067, 1099
BstPAI GACNNNNGTC 1 cut(s) 372
BstPI GGTNACC 2 cut(s) 661, 735
BstSCI CCNGG 1 cut(s) 192
BstSLI GKGCMC 1 cut(s) 77
BstV1I GCAGC 1 cut(s) 150
BstX2I RGATCY 2 cut(s) 88, 1067
BstYI RGATCY 2 cut(s) 88, 1067
BsuRI GGCC 4 cut(s) 75, 230, 511, 1129
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 7 cut(s) 424, 495, 666, 742, 1171, 1216, 1333
BtsI GCAGTG 1 cut(s) 941
BtsIMutI CAGTG 2 cut(s) 922, 941
Cac8I GCNNGC 1 cut(s) 831
Cfr13I GGNCC 5 cut(s) 73, 74, 241, 254, 510
Csp6I GTAC 3 cut(s) 764, 1043, 1194
CspCI CAANNNNNGTGG 4 cut(s) 829, 864, 1234, 1269
CviAII CATG 8 cut(s) 238, 385, 476, 786, 979, 1019, 1055, 1286
CviQI GTAC 3 cut(s) 764, 1043, 1194
DdeI CTNAG 4 cut(s) 96, 423, 642, 759
DpnI GATC 6 cut(s) 90, 519, 729, 879, 1069, 1101
DpnII GATC 6 cut(s) 88, 517, 727, 877, 1067, 1099
DraI TTTAAA 1 cut(s) 885
DriI GACNNNNNGTC 1 cut(s) 334
Eam1105I GACNNNNNGTC 1 cut(s) 334
Eco130I CCWWGG 2 cut(s) 237, 248
Eco147I AGGCCT 1 cut(s) 230
Eco24I GRGCYC 1 cut(s) 77
Eco47I GGWCC 2 cut(s) 241, 254
Eco57I CTGAAG 2 cut(s) 315, 654
Eco91I GGTNACC 2 cut(s) 661, 735
EcoNI CCTNNNNNAGG 1 cut(s) 609
EcoO109I RGGNCCY 1 cut(s) 74
EcoO65I GGTNACC 2 cut(s) 661, 735
EcoRI GAATTC 2 cut(s) 302, 538
EcoT14I CCWWGG 2 cut(s) 237, 248
EcoT38I GRGCYC 1 cut(s) 77
ErhI CCWWGG 2 cut(s) 237, 248
FaeI CATG 8 cut(s) 241, 388, 479, 789, 982, 1022, 1058, 1289
FalI AAGNNNNNCTT 8 cut(s) 197, 229, 478, 510, 843, 875, 1114, 1146
FaqI GGGAC 1 cut(s) 267
FatI CATG 8 cut(s) 237, 384, 475, 785, 978, 1018, 1054, 1285
FauI CCCGC 2 cut(s) 408, 639
FauNDI CATATG 1 cut(s) 149
FbaI TGATCA 1 cut(s) 727
Fnu4HI GCNGC 1 cut(s) 139
FokI GGATG 7 cut(s) 431, 502, 653, 729, 1178, 1203, 1340
FriOI GRGCYC 1 cut(s) 77
Fsp4HI GCNGC 1 cut(s) 139
FspBI CTAG 1 cut(s) 830
GluI GCNGC 1 cut(s) 139
HaeIII GGCC 4 cut(s) 75, 230, 511, 1129
HapII CCGG 1 cut(s) 194
Hin1II CATG 8 cut(s) 241, 388, 479, 789, 982, 1022, 1058, 1289
HincII GTYRAC 1 cut(s) 1336
HindII GTYRAC 1 cut(s) 1336
HindIII AAGCTT 3 cut(s) 636, 1247, 1294
HinfI GANTC 2 cut(s) 388, 720
HpaII CCGG 1 cut(s) 194
HphI GGTGA 3 cut(s) 547, 729, 1130
Hpy166II GTNNAC 2 cut(s) 766, 1336
Hpy188I TCNGA 5 cut(s) 190, 245, 340, 459, 568
Hpy188III TCNNGA 3 cut(s) 270, 502, 632
Hpy8I GTNNAC 2 cut(s) 766, 1336
HpyAV CCTTC 4 cut(s) 421, 638, 845, 1140
HpyCH4III ACNGT 2 cut(s) 917, 1160
HpyCH4IV ACGT 2 cut(s) 891, 1202
HpyCH4V TGCA 9 cut(s) 153, 165, 211, 218, 498, 546, 600, 625, 1061
HpyF3I CTNAG 4 cut(s) 96, 423, 642, 759
HpySE526I ACGT 2 cut(s) 891, 1202
Hsp92II CATG 8 cut(s) 241, 388, 479, 789, 982, 1022, 1058, 1289
Ksp22I TGATCA 1 cut(s) 727
Kzo9I GATC 6 cut(s) 88, 517, 727, 877, 1067, 1099
LmnI GCTCC 2 cut(s) 132, 854
LpnPI CCDG 9 cut(s) 90, 207, 306, 597, 600, 618, 645, 839, 1119
Lsp1109I GCAGC 1 cut(s) 150
LweI GCATC 7 cut(s) 198, 409, 450, 480, 507, 1003, 1266
MaeI CTAG 1 cut(s) 830
MaeII ACGT 2 cut(s) 891, 1202
MaeIII GTNAC 4 cut(s) 344, 607, 661, 735
MalI GATC 6 cut(s) 90, 519, 729, 879, 1069, 1101
MboI GATC 6 cut(s) 88, 517, 727, 877, 1067, 1099
MboII GAAGA 3 cut(s) 83, 308, 1093
MflI RGATCY 2 cut(s) 88, 1067
MhlI GDGCHC 3 cut(s) 77, 815, 859
MmeI TCCRAC 1 cut(s) 268
MnlI CCTC 5 cut(s) 220, 750, 754, 1068, 1071
MseI TTAA 3 cut(s) 579, 884, 1185
MslI CAYNNNNRTG 1 cut(s) 1319
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 1 cut(s) 194
Mva1269I GAATGC 1 cut(s) 141
NciI CCSGG 1 cut(s) 194
NcoI CCATGG 1 cut(s) 237
NdeI CATATG 1 cut(s) 149
NdeII GATC 6 cut(s) 88, 517, 727, 877, 1067, 1099
NheI GCTAGC 1 cut(s) 829
NlaIII CATG 8 cut(s) 241, 388, 479, 789, 982, 1022, 1058, 1289
NlaIV GGNNCC 2 cut(s) 75, 255
NmuCI GTSAC 2 cut(s) 344, 735
PceI AGGCCT 1 cut(s) 230
PctI GAATGC 1 cut(s) 141
PfeI GAWTC 2 cut(s) 388, 720
PkrI GCNGC 1 cut(s) 140
PshAI GACNNNNGTC 1 cut(s) 372
PsiI TTATAA 1 cut(s) 83
PspEI GGTNACC 2 cut(s) 661, 735
PspN4I GGNNCC 2 cut(s) 75, 255
PspOMI GGGCCC 1 cut(s) 73
PspPI GGNCC 5 cut(s) 73, 74, 241, 254, 510
PsuI RGATCY 2 cut(s) 88, 1067
RsaI GTAC 3 cut(s) 765, 1044, 1195
RsaNI GTAC 3 cut(s) 764, 1043, 1194
RseI CAYNNNNRTG 1 cut(s) 1319
SaqAI TTAA 3 cut(s) 579, 884, 1185
SatI GCNGC 1 cut(s) 139
Sau3AI GATC 6 cut(s) 88, 517, 727, 877, 1067, 1099
Sau96I GGNCC 5 cut(s) 73, 74, 241, 254, 510
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 3 cut(s) 77, 815, 859
SfaNI GCATC 7 cut(s) 198, 409, 450, 480, 507, 1003, 1266
SinI GGWCC 2 cut(s) 241, 254
SmiMI CAYNNNNRTG 1 cut(s) 1319
SmlI CTYRAG 2 cut(s) 1244, 1307
SmoI CTYRAG 2 cut(s) 1244, 1307
SseBI AGGCCT 1 cut(s) 230
SsiI CCGC 4 cut(s) 382, 415, 646, 675
SspMI CTAG 1 cut(s) 830
StuI AGGCCT 1 cut(s) 230
StyD4I CCNGG 1 cut(s) 192
StyI CCWWGG 2 cut(s) 237, 248
TaaI ACNGT 2 cut(s) 917, 1160
TaiI ACGT 2 cut(s) 894, 1205
TfiI GAWTC 2 cut(s) 388, 720
Tru1I TTAA 3 cut(s) 579, 884, 1185
Tru9I TTAA 3 cut(s) 579, 884, 1185
TscAI CASTG 2 cut(s) 922, 948
TseFI GTSAC 2 cut(s) 344, 735
TseI GCWGC 1 cut(s) 138
Tsp45I GTSAC 2 cut(s) 344, 735
TspDTI ATGAA 9 cut(s) 17, 69, 315, 401, 449, 774, 891, 1016, 1166
TspGWI ACGGA 1 cut(s) 798
TspRI CASTG 2 cut(s) 922, 948
VpaK11BI GGWCC 2 cut(s) 241, 254
XagI CCTNNNNNAGG 1 cut(s) 609
XapI RAATTY 5 cut(s) 197, 302, 538, 1267, 1351
XcmI CCANNNNNNNNNTGG 1 cut(s) 677
XspI CTAG 1 cut(s) 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.