Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0004545

Physical Location & Seq

Genomic Coordinates Forward (+)

40136304 .. 40144127

Gene Structure 7824 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0327300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2694 bp

ATGGCATCGCAAGATCCGAACACGAACACCGCAAGCGGAAGCGGCAGCGGCAAGCAGGACGAGCCCACCAAACCGCCGCCCCCAAAGGATTCCAAAAAGAAAGATGACAAGAAGGACGAGGATTTGTCCGATGAAGATTTGGCCTTGAAGCAACAATTGGAGTTATATGTGGAGAGGGTTCAAGATCCCGATCCTGGGTTGCAGAAGGTTGCCCTTGAGAGTATGAGGCAGGAAATTCGTACCTCAACTAGTTCCATGACCTCAGTTCCAAAGCCGTTAAAATATCTTCGCCCGCATTATGGAACTCTAAAAGCATATTATGAAACAATGAGTGATTCGGATTTGAAGAAATACCTCGCAGATATATTGTCGGTTTTGGCATTGACCATGTCTGCTGAAGGAGAACGGGAAAGCTTAAAGTTTAGATTATTGGGTTCAGATGGTGATATTGGTTCATGGGGCCATGAATATGTCAGGAACTTAGCTGGAGAAATTGCTCAAGAATATGCCAAGCGACAGTTACAGAGTGATGAGACCCCCTTTGATGATCTAATGGAACTAGTACAACAAATAGTTGCTTTTCACATGAGGCACAATGCTGAACCGGAAGCTGTTGACCTTTTAATGGAGGTGGAAGACCTTGATCTGTTAGTTGAGCATGTTGATAAGACAAATTTTAAGAGGACTTGTCTCTACCTCACCAGTTCGGCAAGATACCTTCCTGGACCAGATGATATGTTAGTTTTGGATATTGCATTCATGATATATCTGAAATTTGAAGAGTATCCAAATGCACTTCAGATTGCTCTTTTCCTCGACAATAAGGAGTATGTAAAGCAAGTCTTTACCTCTTGTGATGATCTGCTGCGTAAAAAGCAATTCTGTTACATCCTTGCCAGACATGGTAGTAATTTTGAGCTTAATGATGAGATGGTTGCAGAAGATGAAGAAAGAGAAGCATTGCAGGATATTATCAATAACGCTAAACTCAGTGAAGGTTATCTTACCCTTGCTCGTGATATTGAGGTCATGGAGGCCAAAACTCCAGAAGATATATACAAGGCACACTTACTTGATGGTCGGGCAAGTGCTGGTGCTAGTGTTGATTCAGCCAGACAGAACTTAGCTGCTACATTTGTTAATGCATTTGTCAATGCTGGCTTTGGTCAGGATAAGTTGATGACTGTCCCATCAGATGCCTCGAGTGGTGGTGCATCAGGAAACTGGCTTTTCAAGAATAAGGAACATGGGAAGACCAGTGCTGCAGCAAGTCTGGGTACAATTTTACTGTGGGATGTTGACTCCGGGCTGGCTCAAATTGACAAGTACTTCCACAGCAATGACAACCATGTTATTGCTGGTGCGTTGTTAGGGGTTGGGATTGTCAATTGCAGTATAAAAAATGATTGTGATCCAGCACTGGCACTTCTGGGCGAATACATAGATAAAGAAGATCCGTCTATCCGAATTGGTGCAATAATGGGTCTAGGGATTGCATATGCTGGTGCTGAGAACGAGCAGATACGTAGTAAGCTGACTCCTATACTAAATGACGCCAAAGCTCCCCTTGATGTTATCACATTCGCTGCAATCTCCTTGGGGTTGATATATGTTGGTTCTTGCAATGAGGAGGTGGCTCAGGCAATTATATTTGCACTAATGGACCGAAGTGACGCTGAGTTGGGGGAGCCCCTTGCTCGCCTAATACCCCTCAGCCTAGGTCTTCTGTACCTTGGGAAGCAGGAAAGTGTTGAGGCCACTGCTGAAGTTTCAAAGACATTTCATGAAAAAATCAGAAAACATTGTGATATGACTCTGCTTTCTTGTGCTTATGCGGGAACAGGAAATGTTCTCAAGGTTCAAAACCTTCTTGGCCATTGTTCACAACATCTTGAGAAGGGTGAAACACACCAGGGCCCTGCTGTGCTTGGTATTGCGATGATAGCAATGGCTGAAGAGTTGGGACTTGAGATGGCAATTCGTTCATTGGAGCATCTTCTACAATATGGAGAACAAAATATCCGTCGGGCAGTTCCTTTGGCTCTTGGTCTCCTTTGCATATCAAATCCAAAGGTCACCGTTATGGACACATTAAGCAGACTTAGCCATGATACGGATTCAGAAGTAGCAATGGCGGCAGTAATTTCCTTAGGTGTGATAGGGGCAGGAACCAACAATGCCAGAATAGCTGCCATGCTTCGCAATCTCTCAAGTTATTACTACAAGGATGCCAGCCTACTCTTCTGTGTGCGAATTGCTCAAGGTCTTGTTCATTTAGGAAAGGGTTTATTGACTCTAAATCCATATCATTCTGATCGCTTCTTGTTATCACCGGCAGCACTTGGTGGAATCATAACCATGCTGCATGCATGTCTTGATATGAAAGCCATCATTTTGGGGAAGTATCATTATGTTCTCTACTTTCTGATATTGGCAATGCAGCCGAGAATGTTGCTAACTGTGGACGAGAATCTAAAGCCGTTGTCTGTCCCTGTTCGGGTCGGCCAAGCTGTGGATGTTGTTGGCCAGGCGGGTCGTCCCAAAACAATTACTGGCTTTCAGACCCACTCAACACCAGTTCTTTTGGCTGCCGGCGATAGAGCTGAGCTAGCGACTGAGAAGTATATTCCCCTTTCCCCAATCCTTGAAGGGTTTGTGATTTTGAAAGAAAATCCAGAGTATCGGGAAGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0002791 GO:0002792 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0006928 GO:0008150 GO:0008283 GO:0009894 GO:0009966 GO:0009987 GO:0010646 GO:0012505 GO:0016020 GO:0016477 GO:0019222 GO:0023051 GO:0030162 GO:0031090 GO:0031323 GO:0031329 GO:0031984 GO:0032268 GO:0032879 GO:0032880 GO:0040011 GO:0042058 GO:0042175 GO:0042176 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048870 GO:0050708 GO:0050709 GO:0050789 GO:0050794 GO:0051046 GO:0051048 GO:0051049 GO:0051051 GO:0051171 GO:0051179 GO:0051223 GO:0051224 GO:0051246 GO:0051674 GO:0060255 GO:0061136 GO:0065007 GO:0070201 GO:0080090 GO:0090087 GO:0098588 GO:0098791 GO:0098827 GO:1901184 GO:1903050 GO:1903362 GO:1903530 GO:1903531 GO:1904950

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rhomboid

Protein Analysis

897

Amino Acids

98.47

Weight (kDa)

5.08

Isoelectric Point (pI)

34.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPN1_RPN2_N	PF17781	53 - 357	5.6e-126	RPN1 N-terminal domain
RPN1_C	PF18051	841 - 894	4.5e-31	26S proteasome non-ATPase regulatory subunit RPN1 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004545)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g27870 FvH4_6g28330
malus_domestica	MD01G1181800.v1.1 MD17G1263300.v1.1
prunus_persica	Prupe.3G111700_v2.0.a1 Prupe.3G112000_v2.0.a1 Prupe.3G112200_v2.0.a1 Prupe.3G112200_v2.0.a1 Prupe.3G112400_v2.0.a1 Prupe.3G112400_v2.0.a1
pyrus_communis	pycom17g26350
rosa_chinensis	RchiOBHm_Chr2g0131131
rosa_laevigata	RLG00000019173 RLG00000019176
rosa_multiflora	Rmu_sc0003629.1_g000070
rosa_roxburghii	Rroxscaffold_2G00113130
rosa_rugosa	Rorug02G0327300
rosa_samantha	Rh2AG347600 Rh2AG348000 Rh2BG355200 Rh2BG355300 Rh2CG333800 Rh2CG333900 Rh2CG334000 Rh2CG335100 Rh2DG373000 Rh2DG373300 Rh2DG374100
rosa_wichuraiana	Rw2G028230 Rw2G028250 Rw2G028280 Rw2G028310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	2512
AclWI	GGATC	5 cut(s)	8, 179, 185, 1406, 1448
AcoI	YGGCCR	3 cut(s)	1873, 2503, 2524
AcsI	RAATTY	3 cut(s)	234, 673, 773
AcuI	CTGAAG	4 cut(s)	417, 782, 1785, 1974
AcyI	GRCGYC	1 cut(s)	1554
AdeI	CACNNNGTG	1 cut(s)	2345
AfaI	GTAC	5 cut(s)	241, 564, 1279, 1328, 1730
AfiI	CCNNNNNNNGG	7 cut(s)	194, 195, 299, 625, 2113, 2497, 2512
AgsI	TTSAA	9 cut(s)	148, 182, 346, 779, 1234, 1773, 1862, 2648, 2665
AhlI	ACTAGT	2 cut(s)	248, 559
AjnI	CCWGG	4 cut(s)	193, 721, 1911, 2526
AjuI	GAANNNNNNNTTGG	2 cut(s)	140, 172
AloI	GAACNNNNNNTCC	2 cut(s)	2004, 2036
Alw26I	GTCTC	3 cut(s)	527, 695, 2054
AlwI	GGATC	5 cut(s)	8, 179, 185, 1406, 1448
Ama87I	CYCGRG	1 cut(s)	1201
AoxI	GGCC	8 cut(s)	141, 460, 1035, 1755, 1873, 1915, 2503, 2524
ApaI	GGGCCC	1 cut(s)	1919
ApoI	RAATTY	3 cut(s)	234, 673, 773
ArsI	GACNNNNNNTTYG	2 cut(s)	2008, 2040
AspA2I	CCTAGG	1 cut(s)	1717
AspS9I	GGNCC	5 cut(s)	460, 725, 1663, 1915, 1916
AsuC2I	CCSGG	1 cut(s)	1306
AsuHPI	GGTGA	5 cut(s)	455, 691, 1913, 2068, 2322
AsuNHI	GCTAGC	1 cut(s)	2608
AvaI	CYCGRG	1 cut(s)	1201
AvaII	GGWCC	2 cut(s)	725, 1663
AvrII	CCTAGG	1 cut(s)	1717
AxyI	CCTNAGG	1 cut(s)	2149
BaeGI	GKGCMC	1 cut(s)	1919
BalI	TGGCCA	2 cut(s)	1875, 2526
BanII	GRGCYC	3 cut(s)	66, 1692, 1919
BauI	CACGAG	1 cut(s)	1014
BbsI	GAAGAC	3 cut(s)	642, 1259, 1715
BbvCI	CCTCAGC	1 cut(s)	1712
BccI	CCATC	6 cut(s)	434, 925, 1070, 1198, 1966, 2396
BceAI	ACGGC	2 cut(s)	259, 2464
BcgI	CGANNNNNNTGC	4 cut(s)	218, 252, 2434, 2468
BciT130I	CCWGG	4 cut(s)	195, 723, 1913, 2528
BciVI	GTATCC	1 cut(s)	795
BcnI	CCSGG	1 cut(s)	1306
BcoDI	GTCTC	3 cut(s)	527, 695, 2054
BcuI	ACTAGT	2 cut(s)	248, 559
BfaI	CTAG	6 cut(s)	249, 560, 1098, 1487, 1718, 2609
BfmI	CTRYAG	1 cut(s)	1263
BfuI	GTATCC	1 cut(s)	795
BlnI	CCTAGG	1 cut(s)	1717
BlpI	GCTNAGC	1 cut(s)	2604
BmcAI	AGTACT	1 cut(s)	1328
Bme1390I	CCNGG	5 cut(s)	195, 723, 1306, 1913, 2528
Bme18I	GGWCC	2 cut(s)	725, 1663
BmeT110I	CYCGRG	1 cut(s)	1201
BmgT120I	GGNCC	5 cut(s)	460, 725, 1663, 1915, 1916
BmiI	GGNNCC	4 cut(s)	461, 1689, 1917, 2170
BmrFI	CCNGG	5 cut(s)	195, 723, 1306, 1913, 2528
BmsI	GCATC	5 cut(s)	14, 1186, 1223, 2002, 2218
BmtI	GCTAGC	1 cut(s)	2612
BpiI	GAAGAC	3 cut(s)	642, 1259, 1715
BpmI	CTGGAG	2 cut(s)	507, 1029
Bpu10I	CCTNAGC	2 cut(s)	1638, 1712
Bpu1102I	GCTNAGC	1 cut(s)	2604
BpuEI	CTTGAG	7 cut(s)	236, 483, 1838, 1913, 1988, 2194, 2244
BpuMI	CCSGG	1 cut(s)	1306
BsaAI	YACGTR	1 cut(s)	1526
BsaBI	GATNNNNATC	2 cut(s)	189, 1410
BsaHI	GRCGYC	1 cut(s)	1554
BsaI	GGTCTC	2 cut(s)	527, 2054
BsaJI	CCNNGG	5 cut(s)	194, 1596, 1717, 1732, 1912
BsaWI	WCCGGW	1 cut(s)	604
Bsc4I	CCNNNNNNNGG	7 cut(s)	194, 195, 299, 625, 2113, 2497, 2512
Bse118I	RCCGGY	2 cut(s)	2332, 2591
Bse1I	ACTGG	6 cut(s)	702, 1229, 1257, 1425, 2557, 2576
Bse21I	CCTNAGG	1 cut(s)	2149
Bse3DI	GCAATG	6 cut(s)	959, 1345, 1630, 1953, 2136, 2442
Bse8I	GATNNNNATC	2 cut(s)	189, 1410
BseBI	CCWGG	4 cut(s)	195, 723, 1913, 2528
BseDI	CCNNGG	5 cut(s)	194, 1596, 1717, 1732, 1912
BseGI	GGATG	4 cut(s)	888, 1300, 2233, 2521
BseJI	GATNNNNATC	2 cut(s)	189, 1410
BseLI	CCNNNNNNNGG	7 cut(s)	194, 195, 299, 625, 2113, 2497, 2512
BseMI	GCAATG	6 cut(s)	959, 1345, 1630, 1953, 2136, 2442
BseMII	CTCAG	8 cut(s)	276, 1003, 1500, 1652, 1668, 1726, 2595, 2607
BseNI	ACTGG	6 cut(s)	702, 1229, 1257, 1425, 2557, 2576
BseRI	GAGGAG	1 cut(s)	1643
BseSI	GKGCMC	1 cut(s)	1919
BshFI	GGCC	8 cut(s)	143, 462, 1037, 1757, 1875, 1917, 2505, 2526
BsiHKCI	CYCGRG	1 cut(s)	1201
BsiSI	CCGG	4 cut(s)	605, 1305, 2333, 2592
BslFI	GGGAC	4 cut(s)	1172, 1977, 2474, 2523
BslI	CCNNNNNNNGG	7 cut(s)	194, 195, 299, 625, 2113, 2497, 2512
BsmAI	GTCTC	3 cut(s)	527, 695, 2054
BsmFI	GGGAC	4 cut(s)	1172, 1977, 2474, 2523
BsmI	GAATGC	1 cut(s)	755
BsnI	GGCC	8 cut(s)	143, 462, 1037, 1757, 1875, 1917, 2505, 2526
Bso31I	GGTCTC	2 cut(s)	527, 2054
BsoBI	CYCGRG	1 cut(s)	1201
Bsp120I	GGGCCC	1 cut(s)	1915
Bsp1286I	GDGCHC	3 cut(s)	66, 1692, 1919
Bsp143I	GATC	9 cut(s)	13, 184, 190, 547, 643, 859, 1411, 1453, 2314
Bsp1720I	GCTNAGC	1 cut(s)	2604
BspANI	GGCC	8 cut(s)	143, 462, 1037, 1757, 1875, 1917, 2505, 2526
BspCNI	CTCAG	8 cut(s)	275, 1002, 1501, 1651, 1669, 1725, 2596, 2608
BspHI	TCATGA	2 cut(s)	759, 1783
BspLI	GGNNCC	4 cut(s)	461, 1689, 1917, 2170
BspMAI	CTGCAG	1 cut(s)	1267
BspOI	GCTAGC	1 cut(s)	2612
BspPI	GGATC	5 cut(s)	8, 179, 185, 1406, 1448
BspTNI	GGTCTC	2 cut(s)	527, 2054
BsrDI	GCAATG	6 cut(s)	959, 1345, 1630, 1953, 2136, 2442
BsrFI	RCCGGY	2 cut(s)	2332, 2591
BsrI	ACTGG	6 cut(s)	702, 1229, 1257, 1425, 2557, 2576
BssAI	RCCGGY	2 cut(s)	2332, 2591
BssECI	CCNNGG	5 cut(s)	194, 1596, 1717, 1732, 1912
BssMI	GATC	9 cut(s)	13, 184, 190, 547, 643, 859, 1411, 1453, 2314
BssNI	GRCGYC	1 cut(s)	1554
BssSI	CACGAG	1 cut(s)	1014
BssT1I	CCWWGG	3 cut(s)	1596, 1717, 1732
Bst2BI	CACGAG	1 cut(s)	1014
Bst2UI	CCWGG	4 cut(s)	195, 723, 1913, 2528
Bst4CI	ACNGT	5 cut(s)	519, 1186, 1290, 2080, 2461
Bst6I	CTCTTC	4 cut(s)	774, 1950, 2246, 2682
BstACI	GRCGYC	1 cut(s)	1554
BstBAI	YACGTR	1 cut(s)	1526
BstEII	GGTNACC	1 cut(s)	2074
BstF5I	GGATG	4 cut(s)	888, 1300, 2233, 2521
BstKTI	GATC	9 cut(s)	16, 187, 193, 550, 646, 862, 1414, 1456, 2317
BstMAI	GTCTC	3 cut(s)	527, 695, 2054
BstMBI	GATC	9 cut(s)	13, 184, 190, 547, 643, 859, 1411, 1453, 2314
BstMWI	GCNNNNNNNGC	9 cut(s)	42, 48, 61, 874, 1943, 2103, 2135, 2186, 2609
BstNI	CCWGG	4 cut(s)	195, 723, 1913, 2528
BstNSI	RCATGY	3 cut(s)	662, 2369, 2373
BstPI	GGTNACC	1 cut(s)	2074
BstSCI	CCNGG	5 cut(s)	193, 721, 1304, 1911, 2526
BstSFI	CTRYAG	1 cut(s)	1263
BstSLI	GKGCMC	1 cut(s)	1919
BstSNI	TACGTA	1 cut(s)	1526
BstV2I	GAAGAC	3 cut(s)	642, 1259, 1715
BstX2I	RGATCY	3 cut(s)	13, 184, 1453
BstXI	CCANNNNNNTGG	1 cut(s)	2395
BstYI	RGATCY	3 cut(s)	13, 184, 1453
Bsu36I	CCTNAGG	1 cut(s)	2149
BsuI	GTATCC	1 cut(s)	795
BsuRI	GGCC	8 cut(s)	143, 462, 1037, 1757, 1875, 1917, 2505, 2526
BtgZI	GCGATG	1 cut(s)	1952
BtsCI	GGATG	4 cut(s)	888, 1300, 2233, 2521
BtsI	GCAGTG	1 cut(s)	1758
BtsIMutI	CAGTG	4 cut(s)	997, 1264, 1418, 1758
CciI	TCATGA	2 cut(s)	759, 1783
Cfr10I	RCCGGY	2 cut(s)	2332, 2591
Cfr13I	GGNCC	5 cut(s)	460, 725, 1663, 1915, 1916
CseI	GACGC	2 cut(s)	1562, 1682
Csp6I	GTAC	5 cut(s)	240, 563, 1278, 1327, 1729
CviQI	GTAC	5 cut(s)	240, 563, 1278, 1327, 1729
DpnI	GATC	9 cut(s)	15, 186, 192, 549, 645, 861, 1413, 1455, 2316
DpnII	GATC	9 cut(s)	13, 184, 190, 547, 643, 859, 1411, 1453, 2314
DraIII	CACNNNGTG	1 cut(s)	2345
EaeI	YGGCCR	3 cut(s)	1873, 2503, 2524
Eam1104I	CTCTTC	4 cut(s)	774, 1950, 2246, 2682
EarI	CTCTTC	4 cut(s)	774, 1950, 2246, 2682
Eco105I	TACGTA	1 cut(s)	1526
Eco130I	CCWWGG	3 cut(s)	1596, 1717, 1732
Eco24I	GRGCYC	3 cut(s)	66, 1692, 1919
Eco31I	GGTCTC	2 cut(s)	527, 2054
Eco47I	GGWCC	2 cut(s)	725, 1663
Eco57I	CTGAAG	4 cut(s)	417, 782, 1785, 1974
Eco81I	CCTNAGG	1 cut(s)	2149
Eco88I	CYCGRG	1 cut(s)	1201
Eco91I	GGTNACC	1 cut(s)	2074
EcoO109I	RGGNCCY	2 cut(s)	1915, 1916
EcoO65I	GGTNACC	1 cut(s)	2074
EcoRII	CCWGG	4 cut(s)	193, 721, 1911, 2526
EcoT14I	CCWWGG	3 cut(s)	1596, 1717, 1732
EcoT22I	ATGCAT	2 cut(s)	1147, 2371
EcoT38I	GRGCYC	3 cut(s)	66, 1692, 1919
ErhI	CCWWGG	3 cut(s)	1596, 1717, 1732
FalI	AAGNNNNNCTT	8 cut(s)	827, 859, 987, 1019, 1052, 1084, 1551, 1583
FaqI	GGGAC	4 cut(s)	1172, 1977, 2474, 2523
FauI	CCCGC	3 cut(s)	300, 1828, 2524
FauNDI	CATATG	1 cut(s)	1498
FokI	GGATG	4 cut(s)	875, 1307, 2240, 2528
FriOI	GRGCYC	3 cut(s)	66, 1692, 1919
FspBI	CTAG	6 cut(s)	249, 560, 1098, 1487, 1718, 2609
GsuI	CTGGAG	2 cut(s)	507, 1029
HaeIII	GGCC	8 cut(s)	143, 462, 1037, 1757, 1875, 1917, 2505, 2526
HapII	CCGG	4 cut(s)	605, 1305, 2333, 2592
HgaI	GACGC	2 cut(s)	1562, 1682
Hin1I	GRCGYC	1 cut(s)	1554
HincII	GTYRAC	2 cut(s)	616, 1300
HindII	GTYRAC	2 cut(s)	616, 1300
HindIII	AAGCTT	1 cut(s)	412
HpaII	CCGG	4 cut(s)	605, 1305, 2333, 2592
HphI	GGTGA	5 cut(s)	455, 691, 1913, 2068, 2322
Hpy166II	GTNNAC	4 cut(s)	616, 1300, 1883, 2464
Hpy8I	GTNNAC	4 cut(s)	616, 1300, 1883, 2464
Hpy99I	CGWCG	1 cut(s)	2028
HpyAV	CCTTC	8 cut(s)	106, 199, 392, 728, 989, 1877, 1891, 2642
HpyCH4III	ACNGT	5 cut(s)	519, 1186, 1290, 2080, 2461
HpyCH4IV	ACGT	1 cut(s)	1525
HpyF10VI	GCNNNNNNNGC	9 cut(s)	42, 48, 61, 874, 1943, 2103, 2135, 2186, 2609
HpySE526I	ACGT	1 cut(s)	1525
Hsp92I	GRCGYC	1 cut(s)	1554
KroI	GCCGGC	1 cut(s)	2591
KroNI	GCCGGC	1 cut(s)	2593
Kzo9I	GATC	9 cut(s)	13, 184, 190, 547, 643, 859, 1411, 1453, 2314
LmnI	GCTCC	3 cut(s)	1567, 1687, 1990
LweI	GCATC	5 cut(s)	14, 1186, 1223, 2002, 2218
MaeI	CTAG	6 cut(s)	249, 560, 1098, 1487, 1718, 2609
MaeII	ACGT	1 cut(s)	1525
MaeIII	GTNAC	4 cut(s)	519, 884, 1670, 2074
MalI	GATC	9 cut(s)	15, 186, 192, 549, 645, 861, 1413, 1455, 2316
MboI	GATC	9 cut(s)	13, 184, 190, 547, 643, 859, 1411, 1453, 2314
MfeI	CAATTG	2 cut(s)	155, 1387
MflI	RGATCY	3 cut(s)	13, 184, 1453
MhlI	GDGCHC	3 cut(s)	66, 1692, 1919
MlsI	TGGCCA	2 cut(s)	1875, 2526
MluNI	TGGCCA	2 cut(s)	1875, 2526
MlyI	GAGTC	4 cut(s)	1295, 1531, 1807, 2287
Mox20I	TGGCCA	2 cut(s)	1875, 2526
Mph1103I	ATGCAT	2 cut(s)	1147, 2371
MroNI	GCCGGC	1 cut(s)	2591
MscI	TGGCCA	2 cut(s)	1875, 2526
MseI	TTAA	7 cut(s)	278, 416, 623, 678, 921, 1140, 2093
MslI	CAYNNNNRTG	4 cut(s)	468, 1338, 2081, 2357
Msp20I	TGGCCA	2 cut(s)	1875, 2526
MspA1I	CMGCKG	1 cut(s)	48
MspI	CCGG	4 cut(s)	605, 1305, 2333, 2592
MspR9I	CCNGG	5 cut(s)	195, 723, 1306, 1913, 2528
MunI	CAATTG	2 cut(s)	155, 1387
Mva1269I	GAATGC	1 cut(s)	755
MvaI	CCWGG	4 cut(s)	195, 723, 1913, 2528
MwoI	GCNNNNNNNGC	9 cut(s)	42, 48, 61, 874, 1943, 2103, 2135, 2186, 2609
NaeI	GCCGGC	1 cut(s)	2593
NciI	CCSGG	1 cut(s)	1306
NdeI	CATATG	1 cut(s)	1498
NdeII	GATC	9 cut(s)	13, 184, 190, 547, 643, 859, 1411, 1453, 2314
NgoMIV	GCCGGC	1 cut(s)	2591
NheI	GCTAGC	1 cut(s)	2608
NlaIV	GGNNCC	4 cut(s)	461, 1689, 1917, 2170
NmeAIII	GCCGAG	1 cut(s)	2469
NmuCI	GTSAC	2 cut(s)	1670, 2074
NsiI	ATGCAT	2 cut(s)	1147, 2371
NspI	RCATGY	3 cut(s)	662, 2369, 2373
PaeI	GCATGC	1 cut(s)	2369
PaeR7I	CTCGAG	1 cut(s)	1201
PagI	TCATGA	2 cut(s)	759, 1783
PcsI	WCGNNNNNNNCGW	1 cut(s)	14
PctI	GAATGC	1 cut(s)	755
PdiI	GCCGGC	1 cut(s)	2593
PfeI	GAWTC	6 cut(s)	89, 335, 1106, 2117, 2349, 2470
PflFI	GACNNNGTC	1 cut(s)	388
PflMI	CCANNNNNTGG	1 cut(s)	2512
PfoI	TCCNGGA	1 cut(s)	721
PleI	GAGTC	4 cut(s)	1295, 1531, 1807, 2287
PpsI	GAGTC	4 cut(s)	1295, 1531, 1807, 2287
Ppu21I	YACGTR	1 cut(s)	1526
Psp6I	CCWGG	4 cut(s)	193, 721, 1911, 2526
PspEI	GGTNACC	1 cut(s)	2074
PspGI	CCWGG	4 cut(s)	193, 721, 1911, 2526
PspN4I	GGNNCC	4 cut(s)	461, 1689, 1917, 2170
PspOMI	GGGCCC	1 cut(s)	1915
PspPI	GGNCC	5 cut(s)	460, 725, 1663, 1915, 1916
PspXI	VCTCGAGB	1 cut(s)	1201
PstI	CTGCAG	1 cut(s)	1267
PsuI	RGATCY	3 cut(s)	13, 184, 1453
PsyI	GACNNNGTC	1 cut(s)	388
RsaI	GTAC	5 cut(s)	241, 564, 1279, 1328, 1730
RsaNI	GTAC	5 cut(s)	240, 563, 1278, 1327, 1729
RseI	CAYNNNNRTG	4 cut(s)	468, 1338, 2081, 2357
SaqAI	TTAA	7 cut(s)	278, 416, 623, 678, 921, 1140, 2093
Sau3AI	GATC	9 cut(s)	13, 184, 190, 547, 643, 859, 1411, 1453, 2314
Sau96I	GGNCC	5 cut(s)	460, 725, 1663, 1915, 1916
ScaI	AGTACT	1 cut(s)	1328
SchI	GAGTC	4 cut(s)	1295, 1531, 1807, 2287
ScrFI	CCNGG	5 cut(s)	195, 723, 1306, 1913, 2528
SduI	GDGCHC	3 cut(s)	66, 1692, 1919
SfaNI	GCATC	5 cut(s)	14, 1186, 1223, 2002, 2218
SfcI	CTRYAG	1 cut(s)	1263
Sfr274I	CTCGAG	1 cut(s)	1201
SinI	GGWCC	2 cut(s)	725, 1663
SlaI	CTCGAG	1 cut(s)	1201
SmiMI	CAYNNNNRTG	4 cut(s)	468, 1338, 2081, 2357
SmlI	CTYRAG	8 cut(s)	215, 498, 1201, 1853, 1892, 1967, 2209, 2259
SmoI	CTYRAG	8 cut(s)	215, 498, 1201, 1853, 1892, 1967, 2209, 2259
SnaBI	TACGTA	1 cut(s)	1526
SpeI	ACTAGT	2 cut(s)	248, 559
SphI	GCATGC	1 cut(s)	2369
SspMI	CTAG	6 cut(s)	249, 560, 1098, 1487, 1718, 2609
StyD4I	CCNGG	5 cut(s)	193, 721, 1304, 1911, 2526
StyI	CCWWGG	3 cut(s)	1596, 1717, 1732
TaaI	ACNGT	5 cut(s)	519, 1186, 1290, 2080, 2461
TaiI	ACGT	1 cut(s)	1528
TaqI	TCGA	2 cut(s)	816, 1202
TaqII	GACCGA	1 cut(s)	1680
TatI	WGTACW	2 cut(s)	562, 1326
TauI	GCSGC	4 cut(s)	45, 51, 79, 2138
TfiI	GAWTC	6 cut(s)	89, 335, 1106, 2117, 2349, 2470
Tru1I	TTAA	7 cut(s)	278, 416, 623, 678, 921, 1140, 2093
Tru9I	TTAA	7 cut(s)	278, 416, 623, 678, 921, 1140, 2093
TscAI	CASTG	4 cut(s)	997, 1264, 1425, 1765
TseFI	GTSAC	2 cut(s)	1670, 2074
Tsp45I	GTSAC	2 cut(s)	1670, 2074
TspGWI	ACGGA	3 cut(s)	1446, 2012, 2129
TspRI	CASTG	4 cut(s)	997, 1264, 1425, 1765
Tth111I	GACNNNGTC	1 cut(s)	388
Van91I	CCANNNNNTGG	1 cut(s)	2512
VpaK11BI	GGWCC	2 cut(s)	725, 1663
XapI	RAATTY	3 cut(s)	234, 673, 773
XceI	RCATGY	3 cut(s)	662, 2369, 2373
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1355
XhoI	CTCGAG	1 cut(s)	1201
XmaJI	CCTAGG	1 cut(s)	1717
XspI	CTAG	6 cut(s)	249, 560, 1098, 1487, 1718, 2609
ZrmI	AGTACT	1 cut(s)	1328
Zsp2I	ATGCAT	2 cut(s)	1147, 2371

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.