Rw2G028230

F-box associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004545
Physical Location & Seq
Reverse (-)
44994448 .. 44997257
2810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G028230.1

Sequence Viewer

Length: 1467 bp
ATGAAAAGGAAAAGGAAATCAATAACTTATCCCACCAAAGTAGGCAACAAAGATGAACACATTGAACAAATGGGCCCTGTTATAACAGATCTTCCAAAGTTTATTTGTCAAATCATTCTACTAAAGCTCCCAACACGCAGCATTCTCATATGCAAATGTGTATGCAAGACTTGGCGTGTGCTAATTTCTGATCCAGAATTTGCCAAGATGCACTTTGCACAAGCAGAGGCCTATCCCATGGTCCGACCTTGGGGACCAACACGGGTGTCAAGAACCCTTTACTTGGTTGAACCTGAAGATGACTTCAGTTTGGCTATAAAGGACAAGGACTATTTGTCCGATTGTGACTATGGCTCATTGAAGTTTGACAAGTGTCATTTCCGCATGAATCTTGCCAAATATAAATTACCATTGCGGGATGTTGAGAAGGTAATGAATAATGACGATAATGTCAATCTGATGCGTAATAGGAAACATGGCACTAAAAGGATGCCTTGCATCAAGATTAGGCCCAACGATCACAAATACAATGTGGTGAATTCGTGCAATGGCTTCCTTTGTTTGTCTGAACCAATTTTTAATGACCCAGTTGTTGTCTGCAACCCTGTAACAGGCGAGTTTATGCACCTTCCTGAAGCTTCTAAGCAGGAGAACCAAAAGGTTACCATCCACTGCGGAATTGGTTTCAGTCCAAAAGCTAACAGATATAAGGTCGTGCGAATCTTTGATAAAGGGTTACCTCATCCTATTACAATGGCTGAGGTACACACTCTTGGGTCAAGTTCATGGAAACTCATTGGTAGTTGTCCCTGCTCATCATATAACCAGCTAGCGTTCCCCACCTATATTGAAGGAGCACTTTATTGGTTTTACTATGATCCTTTAAAATACGTCATACTTTCATTTGACTTGGACAGTGAGAACTTCCAATCACTTCCACCACTGCCTTTTCAACGAGAGCAATATCGTAATGTGAGCATGGGAGTTTTGGGAAATTGTCTTTGTGTCTGTGATGCTCATGGTATTCATATCAATTTGTGGGTACTGAAAAATCATGGTGCAAAAAGATCTTGGAGGAGGAAGATTTCTATTGAAACAGATCATTTTGGGTTTGATGGTGAAAGGTGGCCTTCTGGCTTATATAAACCAATGAAATACTGTAAGGATGGGGGCTTGTTGATGTTTAACTATCGTACTAACGCGTTGATTTACCATCATCCAAAAACCCACAGATTTATTTATCTCAAGCTTCGTGGCATCAAGTCAAATTTTTCAGCTATCAGTCATGTTCCAAGCTTTATTTCACTCAAGGACATTTTGGTGGGGGATGATGTCAACATACTGAATATAAATTCAAGGTGTGCACAGATGAAGCTGCTAGGAGAGACAAGAGCTCTTTTTCTGGAGGAAGAAATTGCAGATTTAGCATCAGACTTTTTCACGTCTAATAGTAGCGATGAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

488

Amino Acids

56.21

Weight (kDa)

8.88

Isoelectric Point (pI)

43.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 28 - 67 1.1e-06 F-box domain
F-box-like PF12937 28 - 67 2e-06 F-box-like
FBA_1 PF07734 167 - 365 2.5e-24 F-box associated beta propeller domain
FBA_3 PF08268 171 - 367 7.5e-23 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 174 - 359 4.8e-07 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 83
AasI GACNNNNNNGTC 1 cut(s) 449
AccII CGCG 1 cut(s) 1202
AciI CCGC 3 cut(s) 382, 415, 675
AclWI GGATC 2 cut(s) 185, 872
AcsI RAATTY 4 cut(s) 197, 538, 1267, 1351
AcuI CTGAAG 3 cut(s) 289, 315, 654
AfaI GTAC 3 cut(s) 765, 1044, 1195
AfiI CCNNNNNNNGG 3 cut(s) 250, 283, 611
AflIII ACRYGT 1 cut(s) 1200
AgsI TTSAA 7 cut(s) 65, 290, 361, 851, 953, 1094, 1356
AhdI GACNNNNNGTC 1 cut(s) 334
AjiI CACGTC 1 cut(s) 1443
AjuI GAANNNNNNNTTGG 2 cut(s) 1285, 1317
AluBI AGCT 9 cut(s) 127, 638, 698, 829, 1249, 1277, 1296, 1375, 1394
AluI AGCT 9 cut(s) 127, 638, 698, 829, 1249, 1277, 1296, 1375, 1394
Alw21I GWGCWC 3 cut(s) 859, 1366, 1396
Alw26I GTCTC 1 cut(s) 1379
Alw44I GTGCAC 1 cut(s) 1362
AlwI GGATC 2 cut(s) 185, 872
AoxI GGCC 4 cut(s) 73, 228, 509, 1127
ApaI GGGCCC 1 cut(s) 77
ApaLI GTGCAC 1 cut(s) 1362
ApeKI GCWGC 2 cut(s) 138, 1375
ApoI RAATTY 4 cut(s) 197, 538, 1267, 1351
AspS9I GGNCC 5 cut(s) 73, 74, 241, 254, 510
AsuHPI GGTGA 2 cut(s) 547, 1130
AsuNHI GCTAGC 1 cut(s) 829
AvaII GGWCC 2 cut(s) 241, 254
BaeGI GKGCMC 2 cut(s) 77, 1366
BanII GRGCYC 2 cut(s) 77, 1396
Bbv12I GWGCWC 3 cut(s) 859, 1366, 1396
BbvCI CCTCAGC 1 cut(s) 759
BbvI GCAGC 2 cut(s) 150, 1362
BccI CCATC 4 cut(s) 674, 1109, 1160, 1221
BcoDI GTCTC 1 cut(s) 1379
BfaI CTAG 2 cut(s) 830, 1379
BglII AGATCT 2 cut(s) 88, 1067
BisI GCNGC 2 cut(s) 139, 1376
BlsI GCNGC 2 cut(s) 140, 1377
Bme18I GGWCC 2 cut(s) 241, 254
BmeRI GACNNNNNGTC 1 cut(s) 334
BmgBI CACGTC 1 cut(s) 1443
BmgT120I GGNCC 5 cut(s) 73, 74, 241, 254, 510
BmiI GGNNCC 2 cut(s) 75, 255
BmrI ACTGGG 1 cut(s) 581
BmsI GCATC 7 cut(s) 198, 450, 480, 507, 1003, 1266, 1436
BmtI GCTAGC 1 cut(s) 833
BmuI ACTGGG 1 cut(s) 581
BoxI GACNNNNGTC 1 cut(s) 372
BpmI CTGGAG 1 cut(s) 1424
Bpu10I CCTNAGC 1 cut(s) 759
BpuEI CTTGAG 2 cut(s) 1229, 1292
BsaJI CCNNGG 2 cut(s) 237, 248
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 3 cut(s) 250, 283, 611
Bse1I ACTGG 1 cut(s) 587
Bse3DI GCAATG 2 cut(s) 410, 553
BseDI CCNNGG 2 cut(s) 237, 248
BseGI GGATG 7 cut(s) 424, 495, 666, 742, 1171, 1216, 1333
BseLI CCNNNNNNNGG 3 cut(s) 250, 283, 611
BseMI GCAATG 2 cut(s) 410, 553
BseMII CTCAG 1 cut(s) 750
BseNI ACTGG 1 cut(s) 587
BseRI GAGGAG 1 cut(s) 1090
BseSI GKGCMC 2 cut(s) 77, 1366
BseXI GCAGC 2 cut(s) 150, 1362
Bsh1236I CGCG 1 cut(s) 1202
BshFI GGCC 4 cut(s) 75, 230, 511, 1129
BsiHKAI GWGCWC 3 cut(s) 859, 1366, 1396
BslFI GGGAC 2 cut(s) 267, 792
BslI CCNNNNNNNGG 3 cut(s) 250, 283, 611
BsmAI GTCTC 1 cut(s) 1379
BsmFI GGGAC 2 cut(s) 267, 792
BsmI GAATGC 1 cut(s) 141
BsnI GGCC 4 cut(s) 75, 230, 511, 1129
Bsp120I GGGCCC 1 cut(s) 73
Bsp1286I GDGCHC 4 cut(s) 77, 859, 1366, 1396
Bsp143I GATC 6 cut(s) 88, 190, 517, 877, 1067, 1099
Bsp19I CCATGG 1 cut(s) 237
BspACI CCGC 3 cut(s) 382, 415, 675
BspANI GGCC 4 cut(s) 75, 230, 511, 1129
BspCNI CTCAG 1 cut(s) 751
BspFNI CGCG 1 cut(s) 1202
BspLI GGNNCC 2 cut(s) 75, 255
BspOI GCTAGC 1 cut(s) 833
BspPI GGATC 2 cut(s) 185, 872
BsrDI GCAATG 2 cut(s) 410, 553
BsrI ACTGG 1 cut(s) 587
BssECI CCNNGG 2 cut(s) 237, 248
BssMI GATC 6 cut(s) 88, 190, 517, 877, 1067, 1099
BssT1I CCWWGG 2 cut(s) 237, 248
Bst4CI ACNGT 2 cut(s) 917, 1160
BstC8I GCNNGC 1 cut(s) 831
BstDEI CTNAG 2 cut(s) 642, 759
BstDSI CCRYGG 1 cut(s) 237
BstEII GGTNACC 2 cut(s) 661, 735
BstENI CCTNNNNNAGG 1 cut(s) 609
BstF5I GGATG 7 cut(s) 424, 495, 666, 742, 1171, 1216, 1333
BstFNI CGCG 1 cut(s) 1202
BstKTI GATC 6 cut(s) 91, 193, 520, 880, 1070, 1102
BstMAI GTCTC 1 cut(s) 1379
BstMBI GATC 6 cut(s) 88, 190, 517, 877, 1067, 1099
BstMWI GCNNNNNNNGC 1 cut(s) 1424
BstPAI GACNNNNGTC 1 cut(s) 372
BstPI GGTNACC 2 cut(s) 661, 735
BstSLI GKGCMC 2 cut(s) 77, 1366
BstUI CGCG 1 cut(s) 1202
BstV1I GCAGC 2 cut(s) 150, 1362
BstX2I RGATCY 2 cut(s) 88, 1067
BstYI RGATCY 2 cut(s) 88, 1067
BsuRI GGCC 4 cut(s) 75, 230, 511, 1129
BtgI CCRYGG 1 cut(s) 237
BtrI CACGTC 1 cut(s) 1443
BtsCI GGATG 7 cut(s) 424, 495, 666, 742, 1171, 1216, 1333
BtsI GCAGTG 2 cut(s) 670, 941
BtsIMutI CAGTG 3 cut(s) 670, 922, 941
Cac8I GCNNGC 1 cut(s) 831
Cfr13I GGNCC 5 cut(s) 73, 74, 241, 254, 510
Csp6I GTAC 3 cut(s) 764, 1043, 1194
CspCI CAANNNNNGTGG 4 cut(s) 829, 864, 1234, 1269
CviAII CATG 8 cut(s) 238, 385, 476, 786, 979, 1019, 1055, 1286
CviQI GTAC 3 cut(s) 764, 1043, 1194
DdeI CTNAG 2 cut(s) 642, 759
DpnI GATC 6 cut(s) 90, 192, 519, 879, 1069, 1101
DpnII GATC 6 cut(s) 88, 190, 517, 877, 1067, 1099
DraI TTTAAA 1 cut(s) 885
DrdI GACNNNNNNGTC 1 cut(s) 449
DriI GACNNNNNGTC 1 cut(s) 334
DseDI GACNNNNNNGTC 1 cut(s) 449
Eam1105I GACNNNNNGTC 1 cut(s) 334
Ecl136II GAGCTC 1 cut(s) 1394
Eco130I CCWWGG 2 cut(s) 237, 248
Eco147I AGGCCT 1 cut(s) 230
Eco24I GRGCYC 2 cut(s) 77, 1396
Eco47I GGWCC 2 cut(s) 241, 254
Eco53kI GAGCTC 1 cut(s) 1394
Eco57I CTGAAG 3 cut(s) 289, 315, 654
Eco91I GGTNACC 2 cut(s) 661, 735
EcoICRI GAGCTC 1 cut(s) 1394
EcoNI CCTNNNNNAGG 1 cut(s) 609
EcoO109I RGGNCCY 1 cut(s) 74
EcoO65I GGTNACC 2 cut(s) 661, 735
EcoRI GAATTC 1 cut(s) 538
EcoT14I CCWWGG 2 cut(s) 237, 248
EcoT38I GRGCYC 2 cut(s) 77, 1396
ErhI CCWWGG 2 cut(s) 237, 248
FaeI CATG 8 cut(s) 241, 388, 479, 789, 982, 1022, 1058, 1289
FalI AAGNNNNNCTT 8 cut(s) 197, 229, 478, 510, 843, 875, 1114, 1146
FaqI GGGAC 2 cut(s) 267, 792
FatI CATG 8 cut(s) 237, 384, 475, 785, 978, 1018, 1054, 1285
FauI CCCGC 1 cut(s) 408
FauNDI CATATG 1 cut(s) 149
Fnu4HI GCNGC 2 cut(s) 139, 1376
FokI GGATG 7 cut(s) 431, 502, 653, 729, 1178, 1203, 1340
FriOI GRGCYC 2 cut(s) 77, 1396
Fsp4HI GCNGC 2 cut(s) 139, 1376
FspBI CTAG 2 cut(s) 830, 1379
GluI GCNGC 2 cut(s) 139, 1376
GsuI CTGGAG 1 cut(s) 1424
HaeIII GGCC 4 cut(s) 75, 230, 511, 1129
Hin1II CATG 8 cut(s) 241, 388, 479, 789, 982, 1022, 1058, 1289
HincII GTYRAC 1 cut(s) 1336
HindII GTYRAC 1 cut(s) 1336
HindIII AAGCTT 3 cut(s) 636, 1247, 1294
HinfI GANTC 2 cut(s) 388, 720
HphI GGTGA 2 cut(s) 547, 1130
Hpy166II GTNNAC 3 cut(s) 766, 1336, 1364
Hpy188I TCNGA 6 cut(s) 190, 245, 340, 459, 568, 1432
Hpy188III TCNNGA 5 cut(s) 194, 270, 502, 632, 1403
Hpy8I GTNNAC 3 cut(s) 766, 1336, 1364
HpyAV CCTTC 4 cut(s) 421, 638, 845, 1140
HpyCH4III ACNGT 2 cut(s) 917, 1160
HpyCH4IV ACGT 2 cut(s) 891, 1442
HpyF10VI GCNNNNNNNGC 1 cut(s) 1424
HpyF3I CTNAG 2 cut(s) 642, 759
HpySE526I ACGT 2 cut(s) 891, 1442
Hsp92II CATG 8 cut(s) 241, 388, 479, 789, 982, 1022, 1058, 1289
Kzo9I GATC 6 cut(s) 88, 190, 517, 877, 1067, 1099
LmnI GCTCC 2 cut(s) 132, 854
Lsp1109I GCAGC 2 cut(s) 150, 1362
LweI GCATC 7 cut(s) 198, 450, 480, 507, 1003, 1266, 1436
MaeI CTAG 2 cut(s) 830, 1379
MaeII ACGT 2 cut(s) 891, 1442
MaeIII GTNAC 4 cut(s) 344, 607, 661, 735
MalI GATC 6 cut(s) 90, 192, 519, 879, 1069, 1101
MboI GATC 6 cut(s) 88, 190, 517, 877, 1067, 1099
MboII GAAGA 4 cut(s) 83, 308, 1093, 1421
MflI RGATCY 2 cut(s) 88, 1067
MhlI GDGCHC 4 cut(s) 77, 859, 1366, 1396
MluI ACGCGT 1 cut(s) 1200
MmeI TCCRAC 1 cut(s) 268
MnlI CCTC 6 cut(s) 220, 750, 754, 1068, 1071, 1399
MseI TTAA 3 cut(s) 579, 884, 1185
MslI CAYNNNNRTG 1 cut(s) 1319
Mva1269I GAATGC 1 cut(s) 141
MvnI CGCG 1 cut(s) 1202
MwoI GCNNNNNNNGC 1 cut(s) 1424
NcoI CCATGG 1 cut(s) 237
NdeI CATATG 1 cut(s) 149
NdeII GATC 6 cut(s) 88, 190, 517, 877, 1067, 1099
NheI GCTAGC 1 cut(s) 829
NlaIII CATG 8 cut(s) 241, 388, 479, 789, 982, 1022, 1058, 1289
NlaIV GGNNCC 2 cut(s) 75, 255
NmuCI GTSAC 1 cut(s) 344
PceI AGGCCT 1 cut(s) 230
PctI GAATGC 1 cut(s) 141
PfeI GAWTC 2 cut(s) 388, 720
PkrI GCNGC 2 cut(s) 140, 1377
PshAI GACNNNNGTC 1 cut(s) 372
PsiI TTATAA 1 cut(s) 83
Psp124BI GAGCTC 1 cut(s) 1396
PspEI GGTNACC 2 cut(s) 661, 735
PspN4I GGNNCC 2 cut(s) 75, 255
PspOMI GGGCCC 1 cut(s) 73
PspPI GGNCC 5 cut(s) 73, 74, 241, 254, 510
PsuI RGATCY 2 cut(s) 88, 1067
RsaI GTAC 3 cut(s) 765, 1044, 1195
RsaNI GTAC 3 cut(s) 764, 1043, 1194
RseI CAYNNNNRTG 1 cut(s) 1319
SacI GAGCTC 1 cut(s) 1396
SaqAI TTAA 3 cut(s) 579, 884, 1185
SatI GCNGC 2 cut(s) 139, 1376
Sau3AI GATC 6 cut(s) 88, 190, 517, 877, 1067, 1099
Sau96I GGNCC 5 cut(s) 73, 74, 241, 254, 510
SduI GDGCHC 4 cut(s) 77, 859, 1366, 1396
SfaNI GCATC 7 cut(s) 198, 450, 480, 507, 1003, 1266, 1436
SinI GGWCC 2 cut(s) 241, 254
SmiMI CAYNNNNRTG 1 cut(s) 1319
SmlI CTYRAG 2 cut(s) 1244, 1307
SmoI CTYRAG 2 cut(s) 1244, 1307
SseBI AGGCCT 1 cut(s) 230
SsiI CCGC 3 cut(s) 382, 415, 675
SspMI CTAG 2 cut(s) 830, 1379
SstI GAGCTC 1 cut(s) 1396
StuI AGGCCT 1 cut(s) 230
StyI CCWWGG 2 cut(s) 237, 248
TaaI ACNGT 2 cut(s) 917, 1160
TaiI ACGT 2 cut(s) 894, 1445
TfiI GAWTC 2 cut(s) 388, 720
Tru1I TTAA 3 cut(s) 579, 884, 1185
Tru9I TTAA 3 cut(s) 579, 884, 1185
TscAI CASTG 3 cut(s) 677, 922, 948
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 2 cut(s) 138, 1375
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 9 cut(s) 17, 69, 401, 449, 774, 891, 1016, 1166, 1385
TspRI CASTG 3 cut(s) 677, 922, 948
VneI GTGCAC 1 cut(s) 1362
VpaK11BI GGWCC 2 cut(s) 241, 254
XagI CCTNNNNNAGG 1 cut(s) 609
XapI RAATTY 4 cut(s) 197, 538, 1267, 1351
XcmI CCANNNNNNNNNTGG 1 cut(s) 677
XspI CTAG 2 cut(s) 830, 1379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.