RLG00000019173

F-box associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004545
Physical Location & Seq
Reverse (-)
47768388 .. 47772749
4362 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019173

Sequence Viewer

Length: 1569 bp
ATGCTTAGCTCTCACAGTACTGGGAATGCTCTCTCTAAGGTTAAGTGGGTCAATCCCCAGCGATCTTGTAGCATTCAGCGTCAACGTTGTGTTCCTAGGATAGCTATGAAAAGGAAAAGGAAGTCAATAGCTTATCCGACCAAAGTAGGCAACAAAGATGAACACATTGAACAAATGGGCCCTGTTATAACAGATCTTCCAAAGTTTATTTGTCAAATCATTCTACTAAAGCTCCCAACACGCAGCATTCTCATATGCAAATGTGTATGCAAGACTTGGCGTGTGCTAATTTCAGACCCGGAATTTGCCAAGATGCACTTTGAACAAGCAGAGGCCTATCCCATGGTCCGACCTTGGGGACCAACACGGGTGTCAAGAACCCTTTACTTGGTTGAACCTGAAGATGACTTCAGTTTGGCTATAAAGGACAAGGACTATTTGTCCGATTGTGACTATGGCTCATTGAAGTTTGACAAGTGTCATTTCCGCATGAATCTTGCCAAATATAAATTACCATTGCGGGATGCTGAGAAGGTAATGAATAATGACGATAATGTCAATCTGATGCGTAATAGGAAACATGGCACTAAAAGGATGCCTTGCATCAAGATTAGGCCCAACGATCACAAATACAATGTGGTGAATTCGTGCAACGGCTTCCTTTGTTTGTCTGAACCAATTTTTAATGACCCAGTTGTTGTCTGCAACCCTGTAACAGGCGAGTTTATGCACCTTCCTGAAGCTTCTAAGCAGGAGAACCAAAAGGTTACCATCCACTGCGGAATTGGTTTCAGTCCAAAAGGTAACAGATATAAGGTGGTGCGAATCTTTGATCAAGGGTCACCTCATCCTGTTACGATGGCTGAGGTACACACTCTTGGGTCAAGTTCATGGAAACTCATTGGAAGTTGTCCCTGCTCATCATATAACCAGCTAGCATTCCCCACCTATATTGAAGGAGCACTTTATTGGTTTTACTATGATTCTTTAAAATATGTCATACTTTCATTTGACTTGGACAGTGAGAACTTCCAATCACTTCCACCACTGCCTTTTCAACGAGAGCAATATCGTAATGTGAGCATGGGAGTTTTGGGAAATTGCCTTTGTGTCTGTGATGCTCATGGTATTCATATCAATTTGTGGGTACTGAAAAATCATGGTGCAAAAAGATCTTGGAGGAGGAAGATTTCTATTGAAACAGATCATTTTGTTGATGGTGAAAGGTGGCCTTCTGGCTTATATAAACCAATGAAATACTGTAAGGATGGGGGCTTGTTGATGTTTAACTATCGAACTAATGCGTTGATTTACCATCACCCAAAAACCCACAGATTTATTTATCTCAAGCTTCGTGGGATCAAGTCAAATTTTTCAGCTATCAGTCATGTTCCAAGCTTTGTTTCTCTCAAGGACATTTTGGTGGGGGATGATGTCAACATCCTGAATATAAATTCAAGGTGTGCACAGATGAAGCTGCTAGGAGAGACAAGAGCTCTTTTTCTGGAGGAAGAAATTGCAGATTTAGCATCAGACTTTTTCACGTCTGATAGTAGCGATGAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

523

Amino Acids

59.95

Weight (kDa)

8.98

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 63 - 102 1.2e-06 F-box domain
F-box-like PF12937 63 - 102 2.1e-06 F-box-like
FBA_1 PF07734 202 - 468 4.5e-25 F-box associated beta propeller domain
FBA_3 PF08268 206 - 406 1.1e-23 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 209 - 394 3.6e-08 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 188
AasI GACNNNNNNGTC 1 cut(s) 554
AciI CCGC 3 cut(s) 487, 520, 780
AclI AACGTT 1 cut(s) 85
AclWI GGATC 1 cut(s) 1367
AcsI RAATTY 4 cut(s) 302, 643, 1369, 1453
AcuI CTGAAG 3 cut(s) 394, 420, 759
AfaI GTAC 3 cut(s) 19, 870, 1149
AfiI CCNNNNNNNGG 3 cut(s) 355, 388, 716
AgsI TTSAA 8 cut(s) 170, 323, 395, 466, 956, 1058, 1199, 1458
AhdI GACNNNNNGTC 1 cut(s) 439
AjiI CACGTC 1 cut(s) 1545
AjuI GAANNNNNNNTTGG 2 cut(s) 1387, 1419
Alw21I GWGCWC 3 cut(s) 964, 1468, 1498
Alw26I GTCTC 1 cut(s) 1481
Alw44I GTGCAC 1 cut(s) 1464
AlwI GGATC 1 cut(s) 1367
AoxI GGCC 4 cut(s) 178, 333, 614, 1229
ApaI GGGCCC 1 cut(s) 182
ApaLI GTGCAC 1 cut(s) 1464
ApeKI GCWGC 2 cut(s) 243, 1477
ApoI RAATTY 4 cut(s) 302, 643, 1369, 1453
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AspA2I CCTAGG 1 cut(s) 95
AspS9I GGNCC 5 cut(s) 178, 179, 346, 359, 615
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 4 cut(s) 652, 834, 1232, 1310
AsuNHI GCTAGC 1 cut(s) 934
AvaII GGWCC 2 cut(s) 346, 359
AvrII CCTAGG 1 cut(s) 95
BaeGI GKGCMC 2 cut(s) 182, 1468
BanII GRGCYC 2 cut(s) 182, 1498
Bbv12I GWGCWC 3 cut(s) 964, 1468, 1498
BbvCI CCTCAGC 1 cut(s) 864
BbvI GCAGC 2 cut(s) 255, 1464
BccI CCATC 5 cut(s) 779, 853, 1211, 1262, 1323
BceAI ACGGC 1 cut(s) 670
BclI TGATCA 1 cut(s) 832
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 1 cut(s) 1481
BfaI CTAG 3 cut(s) 96, 935, 1481
BglII AGATCT 2 cut(s) 193, 1172
BisI GCNGC 2 cut(s) 244, 1478
BlnI CCTAGG 1 cut(s) 95
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 2 cut(s) 245, 1479
BmcAI AGTACT 1 cut(s) 19
Bme1390I CCNGG 1 cut(s) 299
Bme18I GGWCC 2 cut(s) 346, 359
BmeRI GACNNNNNGTC 1 cut(s) 439
BmgBI CACGTC 1 cut(s) 1545
BmgT120I GGNCC 5 cut(s) 178, 179, 346, 359, 615
BmiI GGNNCC 2 cut(s) 180, 360
BmrFI CCNGG 1 cut(s) 299
BmrI ACTGGG 2 cut(s) 30, 686
BmsI GCATC 7 cut(s) 303, 514, 555, 585, 612, 1108, 1538
BmtI GCTAGC 1 cut(s) 938
BmuI ACTGGG 2 cut(s) 30, 686
BoxI GACNNNNGTC 1 cut(s) 477
BpmI CTGGAG 1 cut(s) 1526
Bpu10I CCTNAGC 1 cut(s) 864
Bpu1102I GCTNAGC 1 cut(s) 5
BpuEI CTTGAG 2 cut(s) 1331, 1394
BpuMI CCSGG 1 cut(s) 299
BsaJI CCNNGG 3 cut(s) 95, 342, 353
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 3 cut(s) 355, 388, 716
Bse1I ACTGG 2 cut(s) 25, 692
Bse3DI GCAATG 1 cut(s) 515
BseDI CCNNGG 3 cut(s) 95, 342, 353
BseGI GGATG 7 cut(s) 529, 600, 771, 847, 1273, 1435, 1440
BseLI CCNNNNNNNGG 3 cut(s) 355, 388, 716
BseMI GCAATG 1 cut(s) 515
BseMII CTCAG 2 cut(s) 519, 855
BseNI ACTGG 2 cut(s) 25, 692
BseRI GAGGAG 1 cut(s) 1195
BseSI GKGCMC 2 cut(s) 182, 1468
BseXI GCAGC 2 cut(s) 255, 1464
BseYI CCCAGC 1 cut(s) 57
BshFI GGCC 4 cut(s) 180, 335, 616, 1231
BsiHKAI GWGCWC 3 cut(s) 964, 1468, 1498
BsiSI CCGG 1 cut(s) 299
BslFI GGGAC 2 cut(s) 372, 897
BslI CCNNNNNNNGG 3 cut(s) 355, 388, 716
BsmAI GTCTC 1 cut(s) 1481
BsmFI GGGAC 2 cut(s) 372, 897
BsmI GAATGC 4 cut(s) 31, 72, 246, 938
BsnI GGCC 4 cut(s) 180, 335, 616, 1231
Bsp120I GGGCCC 1 cut(s) 178
Bsp1286I GDGCHC 4 cut(s) 182, 964, 1468, 1498
Bsp143I GATC 7 cut(s) 62, 193, 622, 832, 1172, 1204, 1359
Bsp1720I GCTNAGC 1 cut(s) 5
Bsp19I CCATGG 1 cut(s) 342
BspACI CCGC 3 cut(s) 487, 520, 780
BspANI GGCC 4 cut(s) 180, 335, 616, 1231
BspCNI CTCAG 2 cut(s) 520, 856
BspLI GGNNCC 2 cut(s) 180, 360
BspOI GCTAGC 1 cut(s) 938
BspPI GGATC 1 cut(s) 1367
BsrDI GCAATG 1 cut(s) 515
BsrI ACTGG 2 cut(s) 25, 692
BssECI CCNNGG 3 cut(s) 95, 342, 353
BssMI GATC 7 cut(s) 62, 193, 622, 832, 1172, 1204, 1359
BssT1I CCWWGG 3 cut(s) 95, 342, 353
Bst4CI ACNGT 3 cut(s) 17, 1022, 1262
BstC8I GCNNGC 1 cut(s) 936
BstDEI CTNAG 5 cut(s) 5, 36, 528, 747, 864
BstDSI CCRYGG 1 cut(s) 342
BstEII GGTNACC 2 cut(s) 766, 840
BstENI CCTNNNNNAGG 1 cut(s) 714
BstF5I GGATG 7 cut(s) 529, 600, 771, 847, 1273, 1435, 1440
BstKTI GATC 7 cut(s) 65, 196, 625, 835, 1175, 1207, 1362
BstMAI GTCTC 1 cut(s) 1481
BstMBI GATC 7 cut(s) 62, 193, 622, 832, 1172, 1204, 1359
BstMWI GCNNNNNNNGC 1 cut(s) 1526
BstPAI GACNNNNGTC 1 cut(s) 477
BstPI GGTNACC 2 cut(s) 766, 840
BstSCI CCNGG 1 cut(s) 297
BstSLI GKGCMC 2 cut(s) 182, 1468
BstV1I GCAGC 2 cut(s) 255, 1464
BstX2I RGATCY 2 cut(s) 193, 1172
BstYI RGATCY 2 cut(s) 193, 1172
BsuRI GGCC 4 cut(s) 180, 335, 616, 1231
BtgI CCRYGG 1 cut(s) 342
BtrI CACGTC 1 cut(s) 1545
BtsCI GGATG 7 cut(s) 529, 600, 771, 847, 1273, 1435, 1440
BtsI GCAGTG 2 cut(s) 775, 1046
BtsIMutI CAGTG 3 cut(s) 775, 1027, 1046
Cac8I GCNNGC 1 cut(s) 936
Cfr13I GGNCC 5 cut(s) 178, 179, 346, 359, 615
CseI GACGC 1 cut(s) 68
Csp6I GTAC 3 cut(s) 18, 869, 1148
CspCI CAANNNNNGTGG 4 cut(s) 934, 969, 1336, 1371
CviAII CATG 8 cut(s) 343, 490, 581, 891, 1084, 1124, 1160, 1388
CviQI GTAC 3 cut(s) 18, 869, 1148
DdeI CTNAG 5 cut(s) 5, 36, 528, 747, 864
DpnI GATC 7 cut(s) 64, 195, 624, 834, 1174, 1206, 1361
DpnII GATC 7 cut(s) 62, 193, 622, 832, 1172, 1204, 1359
DraI TTTAAA 1 cut(s) 990
DrdI GACNNNNNNGTC 1 cut(s) 554
DriI GACNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 554
Eam1105I GACNNNNNGTC 1 cut(s) 439
Ecl136II GAGCTC 1 cut(s) 1496
Eco130I CCWWGG 3 cut(s) 95, 342, 353
Eco147I AGGCCT 1 cut(s) 335
Eco24I GRGCYC 2 cut(s) 182, 1498
Eco47I GGWCC 2 cut(s) 346, 359
Eco53kI GAGCTC 1 cut(s) 1496
Eco57I CTGAAG 3 cut(s) 394, 420, 759
Eco91I GGTNACC 2 cut(s) 766, 840
EcoICRI GAGCTC 1 cut(s) 1496
EcoNI CCTNNNNNAGG 1 cut(s) 714
EcoO109I RGGNCCY 1 cut(s) 179
EcoO65I GGTNACC 2 cut(s) 766, 840
EcoRI GAATTC 1 cut(s) 643
EcoT14I CCWWGG 3 cut(s) 95, 342, 353
EcoT38I GRGCYC 2 cut(s) 182, 1498
ErhI CCWWGG 3 cut(s) 95, 342, 353
FaeI CATG 8 cut(s) 346, 493, 584, 894, 1087, 1127, 1163, 1391
FalI AAGNNNNNCTT 8 cut(s) 302, 334, 583, 615, 948, 980, 1216, 1248
FaqI GGGAC 2 cut(s) 372, 897
FatI CATG 8 cut(s) 342, 489, 580, 890, 1083, 1123, 1159, 1387
FauI CCCGC 1 cut(s) 513
FauNDI CATATG 1 cut(s) 254
FbaI TGATCA 1 cut(s) 832
Fnu4HI GCNGC 2 cut(s) 244, 1478
FokI GGATG 7 cut(s) 536, 607, 758, 834, 1280, 1427, 1442
FriOI GRGCYC 2 cut(s) 182, 1498
Fsp4HI GCNGC 2 cut(s) 244, 1478
FspBI CTAG 3 cut(s) 96, 935, 1481
GluI GCNGC 2 cut(s) 244, 1478
GsaI CCCAGC 1 cut(s) 61
GsuI CTGGAG 1 cut(s) 1526
HaeIII GGCC 4 cut(s) 180, 335, 616, 1231
HapII CCGG 1 cut(s) 299
HgaI GACGC 1 cut(s) 68
Hin1II CATG 8 cut(s) 346, 493, 584, 894, 1087, 1127, 1163, 1391
HincII GTYRAC 2 cut(s) 83, 1438
HindII GTYRAC 2 cut(s) 83, 1438
HindIII AAGCTT 3 cut(s) 741, 1349, 1396
HinfI GANTC 3 cut(s) 493, 825, 983
HpaII CCGG 1 cut(s) 299
HphI GGTGA 4 cut(s) 652, 834, 1232, 1310
Hpy166II GTNNAC 4 cut(s) 83, 871, 1438, 1466
Hpy188I TCNGA 8 cut(s) 138, 295, 350, 445, 564, 673, 1534, 1549
Hpy188III TCNNGA 5 cut(s) 375, 607, 737, 1444, 1505
Hpy8I GTNNAC 4 cut(s) 83, 871, 1438, 1466
HpyAV CCTTC 4 cut(s) 526, 743, 950, 1242
HpyCH4III ACNGT 3 cut(s) 17, 1022, 1262
HpyCH4IV ACGT 2 cut(s) 85, 1544
HpyF10VI GCNNNNNNNGC 1 cut(s) 1526
HpyF3I CTNAG 5 cut(s) 5, 36, 528, 747, 864
HpySE526I ACGT 2 cut(s) 85, 1544
Hsp92II CATG 8 cut(s) 346, 493, 584, 894, 1087, 1127, 1163, 1391
Ksp22I TGATCA 1 cut(s) 832
Kzo9I GATC 7 cut(s) 62, 193, 622, 832, 1172, 1204, 1359
LmnI GCTCC 2 cut(s) 237, 959
Lsp1109I GCAGC 2 cut(s) 255, 1464
LweI GCATC 7 cut(s) 303, 514, 555, 585, 612, 1108, 1538
MaeI CTAG 3 cut(s) 96, 935, 1481
MaeII ACGT 2 cut(s) 85, 1544
MaeIII GTNAC 6 cut(s) 449, 712, 766, 803, 840, 853
MalI GATC 7 cut(s) 64, 195, 624, 834, 1174, 1206, 1361
MboI GATC 7 cut(s) 62, 193, 622, 832, 1172, 1204, 1359
MboII GAAGA 4 cut(s) 188, 413, 1198, 1523
MflI RGATCY 2 cut(s) 193, 1172
MhlI GDGCHC 4 cut(s) 182, 964, 1468, 1498
MmeI TCCRAC 2 cut(s) 161, 373
MnlI CCTC 6 cut(s) 325, 855, 859, 1173, 1176, 1501
MseI TTAA 4 cut(s) 42, 684, 989, 1287
MslI CAYNNNNRTG 1 cut(s) 1421
MspI CCGG 1 cut(s) 299
MspR9I CCNGG 1 cut(s) 299
Mva1269I GAATGC 4 cut(s) 31, 72, 246, 938
MwoI GCNNNNNNNGC 1 cut(s) 1526
NciI CCSGG 1 cut(s) 299
NcoI CCATGG 1 cut(s) 342
NdeI CATATG 1 cut(s) 254
NdeII GATC 7 cut(s) 62, 193, 622, 832, 1172, 1204, 1359
NheI GCTAGC 1 cut(s) 934
NlaIII CATG 8 cut(s) 346, 493, 584, 894, 1087, 1127, 1163, 1391
NlaIV GGNNCC 2 cut(s) 180, 360
NmuCI GTSAC 2 cut(s) 449, 840
PceI AGGCCT 1 cut(s) 335
PctI GAATGC 4 cut(s) 31, 72, 246, 938
PfeI GAWTC 3 cut(s) 493, 825, 983
PkrI GCNGC 2 cut(s) 245, 1479
PshAI GACNNNNGTC 1 cut(s) 477
PsiI TTATAA 1 cut(s) 188
Psp124BI GAGCTC 1 cut(s) 1498
Psp1406I AACGTT 1 cut(s) 85
PspEI GGTNACC 2 cut(s) 766, 840
PspFI CCCAGC 1 cut(s) 57
PspN4I GGNNCC 2 cut(s) 180, 360
PspOMI GGGCCC 1 cut(s) 178
PspPI GGNCC 5 cut(s) 178, 179, 346, 359, 615
PsuI RGATCY 2 cut(s) 193, 1172
RsaI GTAC 3 cut(s) 19, 870, 1149
RsaNI GTAC 3 cut(s) 18, 869, 1148
RseI CAYNNNNRTG 1 cut(s) 1421
SacI GAGCTC 1 cut(s) 1498
SaqAI TTAA 4 cut(s) 42, 684, 989, 1287
SatI GCNGC 2 cut(s) 244, 1478
Sau3AI GATC 7 cut(s) 62, 193, 622, 832, 1172, 1204, 1359
Sau96I GGNCC 5 cut(s) 178, 179, 346, 359, 615
ScaI AGTACT 1 cut(s) 19
ScrFI CCNGG 1 cut(s) 299
SduI GDGCHC 4 cut(s) 182, 964, 1468, 1498
SfaNI GCATC 7 cut(s) 303, 514, 555, 585, 612, 1108, 1538
SinI GGWCC 2 cut(s) 346, 359
SmiMI CAYNNNNRTG 1 cut(s) 1421
SmlI CTYRAG 2 cut(s) 1346, 1409
SmoI CTYRAG 2 cut(s) 1346, 1409
SseBI AGGCCT 1 cut(s) 335
SsiI CCGC 3 cut(s) 487, 520, 780
SspMI CTAG 3 cut(s) 96, 935, 1481
SstI GAGCTC 1 cut(s) 1498
StuI AGGCCT 1 cut(s) 335
StyD4I CCNGG 1 cut(s) 297
StyI CCWWGG 3 cut(s) 95, 342, 353
TaaI ACNGT 3 cut(s) 17, 1022, 1262
TaiI ACGT 2 cut(s) 88, 1547
TaqI TCGA 1 cut(s) 1294
TatI WGTACW 1 cut(s) 17
TfiI GAWTC 3 cut(s) 493, 825, 983
Tru1I TTAA 4 cut(s) 42, 684, 989, 1287
Tru9I TTAA 4 cut(s) 42, 684, 989, 1287
TscAI CASTG 3 cut(s) 782, 1027, 1053
TseFI GTSAC 2 cut(s) 449, 840
TseI GCWGC 2 cut(s) 243, 1477
Tsp45I GTSAC 2 cut(s) 449, 840
TspDTI ATGAA 9 cut(s) 122, 174, 506, 554, 879, 996, 1121, 1268, 1487
TspRI CASTG 3 cut(s) 782, 1027, 1053
VneI GTGCAC 1 cut(s) 1464
VpaK11BI GGWCC 2 cut(s) 346, 359
XagI CCTNNNNNAGG 1 cut(s) 714
XapI RAATTY 4 cut(s) 302, 643, 1369, 1453
XcmI CCANNNNNNNNNTGG 1 cut(s) 782
XmaJI CCTAGG 1 cut(s) 95
XspI CTAG 3 cut(s) 96, 935, 1481
ZrmI AGTACT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.