pycom02g01270

glycerol-3-phosphate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001726
Physical Location & Seq
Forward (+)
726930 .. 728623
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g01270.1

Sequence Viewer

Length: 1539 bp
ATGCGATCCGAAACCCTAGCCCTAGCTCCAGCTTTCAATTTTTTCCCAAATCTCAAACCCCCACACAAAACCCTAGCTTTCCACCAAATTTCAGTCCTAATCATCACCTTCCTCGCCTATGCTTCCTTCCATGCCTCCAGAAAGCCCCCAAGCATTGTCAAGAGCGTGCTTGGGCCCACAGTTCTGGCCAATTCGAGCTCCGTCAACACGGGGTGGGCTCCCTTTGATGGTCCCCGGGGCACCCACCGGCTAGGAGAGCTTGATCGATCGTTTCTTTCTGCGTATTCGATTGGAATGTACTTTGCAGGGCATGTCGGTGATCGGATTGATTTGAGGTTGTTTCTTGTGTTTGGGATGATGTGTAGTGGCATTTTAACGATATTTTTCGGGTTAGGATATTGGTTTGATGTTCATGTATTGGGGTACTTTATGGCGGTTCAACTTCTTTGTGGATTGGTTCAGTCGATTGGGTGGCCCTGTGTGGTGGCTATAGTGGGAAACTGGTTTAAGAAAGGGAAGAGGGGGTTGATCATGGGGGTCTGGAGCTCGCATACCTCGGTTGGAAACATCATTGGGTCGGTTGTGGCATCTGGGGTCTTGGAATTCGGATGGGGTTGGTCCTTTGTGGTGCCCGGGGTTTTGGTGATTTTGGTTGGGATTGTGGTGTTTTTGTTTCTTCCTGTGTGTCCAGAAGACTTGGGGTTTGAGTCTCCGGTGAAGGAGATTCAGATGAATGTGGAAGTTAATGGCGTGGAGAATTTGGAGGAGAAAGTAGAATCAGAGGAAGCAGGGCTTCTTGGATCAGAGACTGCAGATTTTGGAACAGAGAATGCAGATGCCGATGCAGACACAGGTTCTTTGGCTGCAGTTGGGTTTCTAGAGGCATGGAGGTTACCAGGTGTGGCGCCATTTGCTTTCTGCCTCTTCTTTTCGAAGCTTGTGTCTTACACTTTCCTTTATTGGTTGCCCTTTTACATAAGGCACACAGCTGTTGCCGGCATGCATTTGTCACACAAAACTGCTGGGAACCTTTCAGCCATATTCGATATTGGAGGAGTCTTTGGTGGAATTTTAGCAGGATTGATATCTGATATGCTTGAAGCTCGAGCTGTAACCTCAATCGCGTTTTTGTTACTTTCGGTTCCAGCCCTTGTTCTCTACCGGCTTTATGGAAGCCTATCGATGGTCGCCAACATTGTTTTGATGTTTCTTTCGGGATTGCTGGTGAATGGCCCTTACTCACTCATCACAACAGCCGTTGCTGCTGATCTTGGTACGCAGACCACGATCAAAGGAAATTCCCGAGCATTAGCCACTGTAACTGCAATCATAGACGGAACAGGTTCTGTTGGGGCAGCTCTTGGCCCGCTTTTGGCCGGGTATACCTCCACGACGGGGTGGAACAACGTGTTTGTTATGCTGATTCTTGCTATTTTCTGTGCAACTTTGTTTTTGATTCGGATTGCGAGAACCGAGATTAAAGGGAAATTGAATGAGGGAAAATGGTCTTTATATAGCCGCATCGGAGGCACACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0042592 GO:0048878 GO:0050801 GO:0055062 GO:0055081 GO:0055083 GO:0065007 GO:0065008 GO:0072505 GO:0072506 GO:0098771
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1

Protein Analysis

513

Amino Acids

54.91

Weight (kDa)

6.55

Isoelectric Point (pI)

26.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 91 - 423 1.1e-36 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 239, 628, 904
AccI GTMKAC 1 cut(s) 1382
AccII CGCG 1 cut(s) 1124
AciI CCGC 3 cut(s) 434, 1367, 1519
AclWI GGATC 1 cut(s) 808
AcoI YGGCCR 2 cut(s) 186, 1374
AcsI RAATTY 5 cut(s) 87, 602, 757, 1068, 1297
AcyI GRCGYC 1 cut(s) 905
AdeI CACNNNGTG 2 cut(s) 213, 1536
AfaI GTAC 3 cut(s) 299, 425, 1276
AfiI CCNNNNNNNGG 4 cut(s) 227, 1183, 1372, 1395
AflIII ACRYGT 1 cut(s) 1407
AgsI TTSAA 4 cut(s) 37, 440, 1100, 1492
AjnI CCWGG 1 cut(s) 895
Alw21I GWGCWC 2 cut(s) 200, 548
Alw26I GTCTC 2 cut(s) 714, 800
AlwI GGATC 1 cut(s) 808
AlwNI CAGNNNCTG 2 cut(s) 809, 1346
Ama87I CYCGRG 4 cut(s) 234, 632, 1104, 1302
AoxI GGCC 6 cut(s) 173, 186, 473, 1231, 1363, 1374
ApaI GGGCCC 1 cut(s) 177
ApeKI GCWGC 3 cut(s) 863, 1262, 1355
ApoI RAATTY 5 cut(s) 87, 602, 757, 1068, 1297
ArsI GACNNNNNNTTYG 4 cut(s) 686, 718, 926, 958
Asp700I GAANNNNTTC 1 cut(s) 1342
AspLEI GCGC 1 cut(s) 907
AspS9I GGNCC 7 cut(s) 173, 174, 230, 474, 618, 1232, 1364
AsuC2I CCSGG 5 cut(s) 235, 236, 633, 634, 1378
AsuHPI GGTGA 5 cut(s) 97, 329, 655, 727, 1237
AsuII TTCGAA 1 cut(s) 932
AvaI CYCGRG 4 cut(s) 234, 632, 1104, 1302
AvaII GGWCC 2 cut(s) 230, 618
BaeGI GKGCMC 3 cut(s) 177, 242, 633
BalI TGGCCA 1 cut(s) 188
BanI GGYRCC 3 cut(s) 239, 628, 904
BanII GRGCYC 4 cut(s) 177, 200, 220, 548
BbsI GAAGAC 1 cut(s) 699
Bbv12I GWGCWC 2 cut(s) 200, 548
BbvI GCAGC 3 cut(s) 850, 1249, 1367
BccI CCATC 3 cut(s) 221, 603, 1177
BceAI ACGGC 1 cut(s) 1241
BciT130I CCWGG 1 cut(s) 897
BclI TGATCA 1 cut(s) 528
BcnI CCSGG 5 cut(s) 235, 236, 633, 634, 1378
BcoDI GTCTC 2 cut(s) 714, 800
BfaI CTAG 5 cut(s) 17, 23, 74, 251, 878
BfmI CTRYAG 3 cut(s) 489, 810, 864
BfoI RGCGCY 1 cut(s) 908
BisI GCNGC 4 cut(s) 864, 1263, 1356, 1519
BlsI GCNGC 4 cut(s) 865, 1264, 1357, 1520
Bme1390I CCNGG 6 cut(s) 235, 236, 633, 634, 897, 1378
Bme18I GGWCC 2 cut(s) 230, 618
BmeT110I CYCGRG 4 cut(s) 234, 632, 1104, 1302
BmgT120I GGNCC 7 cut(s) 173, 174, 230, 474, 618, 1232, 1364
BmiI GGNNCC 8 cut(s) 175, 219, 232, 241, 630, 906, 1028, 1143
BmrFI CCNGG 6 cut(s) 235, 236, 633, 634, 897, 1378
BmsI GCATC 4 cut(s) 596, 826, 832, 1530
BpiI GAAGAC 1 cut(s) 699
BpmI CTGGAG 3 cut(s) 12, 121, 562
Bpu14I TTCGAA 1 cut(s) 932
BpuMI CCSGG 5 cut(s) 235, 236, 633, 634, 1378
Bsa29I ATCGAT 2 cut(s) 265, 1181
BsaBI GATNNNNATC 1 cut(s) 1084
BsaHI GRCGYC 1 cut(s) 905
BsaJI CCNNGG 6 cut(s) 233, 234, 235, 555, 632, 633
BsaWI WCCGGW 1 cut(s) 712
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 1183, 1372, 1395
Bse118I RCCGGY 3 cut(s) 246, 995, 1161
Bse1I ACTGG 1 cut(s) 506
Bse8I GATNNNNATC 1 cut(s) 1084
BseBI CCWGG 1 cut(s) 897
BseCI ATCGAT 2 cut(s) 265, 1181
BseDI CCNNGG 6 cut(s) 233, 234, 235, 555, 632, 633
BseGI GGATG 2 cut(s) 360, 614
BseJI GATNNNNATC 1 cut(s) 1084
BseLI CCNNNNNNNGG 4 cut(s) 227, 1183, 1372, 1395
BseNI ACTGG 1 cut(s) 506
BseRI GAGGAG 2 cut(s) 779, 1068
BseSI GKGCMC 3 cut(s) 177, 242, 633
BseXI GCAGC 3 cut(s) 850, 1249, 1367
BseYI CCCAGC 1 cut(s) 1022
Bsh1236I CGCG 1 cut(s) 1124
Bsh1285I CGRYCG 1 cut(s) 269
BshFI GGCC 6 cut(s) 175, 188, 475, 1233, 1365, 1376
BshNI GGYRCC 3 cut(s) 239, 628, 904
BshVI ATCGAT 2 cut(s) 265, 1181
BsiEI CGRYCG 1 cut(s) 269
BsiHKAI GWGCWC 2 cut(s) 200, 548
BsiHKCI CYCGRG 4 cut(s) 234, 632, 1104, 1302
BsiSI CCGG 7 cut(s) 235, 247, 633, 713, 996, 1162, 1377
BslFI GGGAC 1 cut(s) 216
BslI CCNNNNNNNGG 4 cut(s) 227, 1183, 1372, 1395
BsmAI GTCTC 2 cut(s) 714, 800
BsmFI GGGAC 1 cut(s) 216
BsmI GAATGC 1 cut(s) 835
BsnI GGCC 6 cut(s) 175, 188, 475, 1233, 1365, 1376
BsoBI CYCGRG 4 cut(s) 234, 632, 1104, 1302
Bsp119I TTCGAA 1 cut(s) 932
Bsp120I GGGCCC 1 cut(s) 173
Bsp1286I GDGCHC 6 cut(s) 177, 200, 220, 242, 548, 633
Bsp143I GATC 8 cut(s) 5, 262, 266, 319, 528, 800, 1267, 1287
BspACI CCGC 3 cut(s) 434, 1367, 1519
BspANI GGCC 6 cut(s) 175, 188, 475, 1233, 1365, 1376
BspDI ATCGAT 2 cut(s) 265, 1181
BspFNI CGCG 1 cut(s) 1124
BspLI GGNNCC 8 cut(s) 175, 219, 232, 241, 630, 906, 1028, 1143
BspMAI CTGCAG 2 cut(s) 814, 868
BspPI GGATC 1 cut(s) 808
BspT104I TTCGAA 1 cut(s) 932
BspT107I GGYRCC 3 cut(s) 239, 628, 904
BsrFI RCCGGY 3 cut(s) 246, 995, 1161
BsrI ACTGG 1 cut(s) 506
BssAI RCCGGY 3 cut(s) 246, 995, 1161
BssECI CCNNGG 6 cut(s) 233, 234, 235, 555, 632, 633
BssMI GATC 8 cut(s) 5, 262, 266, 319, 528, 800, 1267, 1287
BssNAI GTATAC 1 cut(s) 1383
BssNI GRCGYC 1 cut(s) 905
Bst1107I GTATAC 1 cut(s) 1383
Bst2UI CCWGG 1 cut(s) 897
Bst4CI ACNGT 3 cut(s) 181, 1318, 1536
Bst6I CTCTTC 2 cut(s) 512, 929
BstACI GRCGYC 1 cut(s) 905
BstBI TTCGAA 1 cut(s) 932
BstC8I GCNNGC 5 cut(s) 167, 548, 997, 1001, 1367
BstEII GGTNACC 1 cut(s) 891
BstF5I GGATG 2 cut(s) 360, 614
BstFNI CGCG 1 cut(s) 1124
BstH2I RGCGCY 1 cut(s) 908
BstHHI GCGC 1 cut(s) 907
BstKTI GATC 8 cut(s) 8, 265, 269, 322, 531, 803, 1270, 1290
BstMAI GTCTC 2 cut(s) 714, 800
BstMBI GATC 8 cut(s) 5, 262, 266, 319, 528, 800, 1267, 1287
BstMCI CGRYCG 1 cut(s) 269
BstMWI GCNNNNNNNGC 4 cut(s) 256, 911, 1262, 1527
BstNI CCWGG 1 cut(s) 897
BstNSI RCATGY 2 cut(s) 314, 1003
BstPI GGTNACC 1 cut(s) 891
BstSCI CCNGG 6 cut(s) 233, 234, 631, 632, 895, 1376
BstSFI CTRYAG 3 cut(s) 489, 810, 864
BstSLI GKGCMC 3 cut(s) 177, 242, 633
BstUI CGCG 1 cut(s) 1124
BstV1I GCAGC 3 cut(s) 850, 1249, 1367
BstV2I GAAGAC 1 cut(s) 699
BstXI CCANNNNNNTGG 1 cut(s) 184
BstZ17I GTATAC 1 cut(s) 1383
Bsu15I ATCGAT 2 cut(s) 265, 1181
BsuRI GGCC 6 cut(s) 175, 188, 475, 1233, 1365, 1376
BsuTUI ATCGAT 2 cut(s) 265, 1181
BtsCI GGATG 2 cut(s) 360, 614
BtsIMutI CAGTG 1 cut(s) 1314
Cac8I GCNNGC 5 cut(s) 167, 548, 997, 1001, 1367
CaiI CAGNNNCTG 2 cut(s) 809, 1346
CfoI GCGC 1 cut(s) 907
Cfr10I RCCGGY 3 cut(s) 246, 995, 1161
Cfr13I GGNCC 7 cut(s) 173, 174, 230, 474, 618, 1232, 1364
Cfr9I CCCGGG 2 cut(s) 234, 632
ClaI ATCGAT 2 cut(s) 265, 1181
CsiI ACCWGGT 1 cut(s) 895
Csp6I GTAC 3 cut(s) 298, 424, 1275
CviAII CATG 6 cut(s) 131, 311, 413, 532, 885, 1000
CviQI GTAC 3 cut(s) 298, 424, 1275
DinI GGCGCC 1 cut(s) 906
DpnI GATC 8 cut(s) 7, 264, 268, 321, 530, 802, 1269, 1289
DpnII GATC 8 cut(s) 5, 262, 266, 319, 528, 800, 1267, 1287
DraIII CACNNNGTG 2 cut(s) 213, 1536
EaeI YGGCCR 2 cut(s) 186, 1374
Eam1104I CTCTTC 2 cut(s) 512, 929
EarI CTCTTC 2 cut(s) 512, 929
Ecl136II GAGCTC 2 cut(s) 198, 546
Eco24I GRGCYC 4 cut(s) 177, 200, 220, 548
Eco32I GATATC 1 cut(s) 1086
Eco47I GGWCC 2 cut(s) 230, 618
Eco53kI GAGCTC 2 cut(s) 198, 546
Eco88I CYCGRG 4 cut(s) 234, 632, 1104, 1302
Eco91I GGTNACC 1 cut(s) 891
EcoICRI GAGCTC 2 cut(s) 198, 546
EcoO65I GGTNACC 1 cut(s) 891
EcoRI GAATTC 1 cut(s) 602
EcoRII CCWGG 1 cut(s) 895
EcoRV GATATC 1 cut(s) 1086
EcoT22I ATGCAT 1 cut(s) 1005
EcoT38I GRGCYC 4 cut(s) 177, 200, 220, 548
EgeI GGCGCC 1 cut(s) 906
EheI GGCGCC 1 cut(s) 906
FaeI CATG 6 cut(s) 134, 314, 416, 535, 888, 1003
FalI AAGNNNNNCTT 2 cut(s) 777, 809
FaqI GGGAC 1 cut(s) 216
FatI CATG 6 cut(s) 130, 310, 412, 531, 884, 999
FauI CCCGC 1 cut(s) 1374
FbaI TGATCA 1 cut(s) 528
FblI GTMKAC 1 cut(s) 1382
Fnu4HI GCNGC 4 cut(s) 864, 1263, 1356, 1519
FokI GGATG 2 cut(s) 367, 621
FriOI GRGCYC 4 cut(s) 177, 200, 220, 548
Fsp4HI GCNGC 4 cut(s) 864, 1263, 1356, 1519
FspBI CTAG 5 cut(s) 17, 23, 74, 251, 878
GlaI GCGC 1 cut(s) 906
GluI GCNGC 4 cut(s) 864, 1263, 1356, 1519
GsaI CCCAGC 1 cut(s) 1026
GsuI CTGGAG 3 cut(s) 12, 121, 562
HaeII RGCGCY 1 cut(s) 908
HaeIII GGCC 6 cut(s) 175, 188, 475, 1233, 1365, 1376
HapII CCGG 7 cut(s) 235, 247, 633, 713, 996, 1162, 1377
HhaI GCGC 1 cut(s) 907
Hin1I GRCGYC 1 cut(s) 905
Hin1II CATG 6 cut(s) 134, 314, 416, 535, 888, 1003
Hin6I GCGC 1 cut(s) 905
HinP1I GCGC 1 cut(s) 905
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 935
HinfI GANTC 6 cut(s) 707, 724, 776, 1056, 1423, 1456
HpaII CCGG 7 cut(s) 235, 247, 633, 713, 996, 1162, 1377
HphI GGTGA 5 cut(s) 97, 329, 655, 727, 1237
Hpy166II GTNNAC 2 cut(s) 205, 1383
Hpy188I TCNGA 9 cut(s) 10, 324, 608, 729, 781, 805, 1090, 1461, 1526
Hpy188III TCNNGA 7 cut(s) 138, 160, 541, 689, 878, 1215, 1302
Hpy8I GTNNAC 2 cut(s) 205, 1383
Hpy99I CGWCG 1 cut(s) 1396
HpyAV CCTTC 3 cut(s) 118, 136, 712
HpyCH4III ACNGT 3 cut(s) 181, 1318, 1536
HpyCH4IV ACGT 1 cut(s) 1407
HpyCH4V TGCA 8 cut(s) 305, 812, 833, 845, 866, 1003, 1325, 1442
HpyF10VI GCNNNNNNNGC 4 cut(s) 256, 911, 1262, 1527
HpySE526I ACGT 1 cut(s) 1407
Hsp92I GRCGYC 1 cut(s) 905
Hsp92II CATG 6 cut(s) 134, 314, 416, 535, 888, 1003
HspAI GCGC 1 cut(s) 905
KasI GGCGCC 1 cut(s) 904
KroI GCCGGC 1 cut(s) 995
KroNI GCCGGC 1 cut(s) 997
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 8 cut(s) 5, 262, 266, 319, 528, 800, 1267, 1287
LmnI GCTCC 4 cut(s) 31, 203, 223, 543
Lsp1109I GCAGC 3 cut(s) 850, 1249, 1367
LweI GCATC 4 cut(s) 596, 826, 832, 1530
MabI ACCWGGT 1 cut(s) 895
MaeI CTAG 5 cut(s) 17, 23, 74, 251, 878
MaeII ACGT 1 cut(s) 1407
MaeIII GTNAC 5 cut(s) 891, 1008, 1111, 1131, 1318
MalI GATC 8 cut(s) 7, 264, 268, 321, 530, 802, 1269, 1289
MboI GATC 8 cut(s) 5, 262, 266, 319, 528, 800, 1267, 1287
MboII GAAGA 4 cut(s) 529, 668, 704, 916
MhlI GDGCHC 6 cut(s) 177, 200, 220, 242, 548, 633
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 8 cut(s) 37, 87, 190, 602, 757, 1068, 1297, 1487
MluNI TGGCCA 1 cut(s) 188
Mly113I GGCGCC 1 cut(s) 905
MlyI GAGTC 2 cut(s) 716, 1065
MmeI TCCRAC 1 cut(s) 541
Mox20I TGGCCA 1 cut(s) 188
Mph1103I ATGCAT 1 cut(s) 1005
MroNI GCCGGC 1 cut(s) 995
MroXI GAANNNNTTC 1 cut(s) 1342
MscI TGGCCA 1 cut(s) 188
MseI TTAA 4 cut(s) 374, 507, 744, 1479
MslI CAYNNNNRTG 1 cut(s) 315
Msp20I TGGCCA 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 989
MspI CCGG 7 cut(s) 235, 247, 633, 713, 996, 1162, 1377
MspR9I CCNGG 6 cut(s) 235, 236, 633, 634, 897, 1378
Mva1269I GAATGC 1 cut(s) 835
MvaI CCWGG 1 cut(s) 897
MvnI CGCG 1 cut(s) 1124
MwoI GCNNNNNNNGC 4 cut(s) 256, 911, 1262, 1527
NaeI GCCGGC 1 cut(s) 997
NarI GGCGCC 1 cut(s) 905
NciI CCSGG 5 cut(s) 235, 236, 633, 634, 1378
NdeII GATC 8 cut(s) 5, 262, 266, 319, 528, 800, 1267, 1287
NgoMIV GCCGGC 1 cut(s) 995
NlaIII CATG 6 cut(s) 134, 314, 416, 535, 888, 1003
NlaIV GGNNCC 8 cut(s) 175, 219, 232, 241, 630, 906, 1028, 1143
NmuCI GTSAC 1 cut(s) 1008
NsiI ATGCAT 1 cut(s) 1005
NspI RCATGY 2 cut(s) 314, 1003
NspV TTCGAA 1 cut(s) 932
PaeI GCATGC 1 cut(s) 1003
PaeR7I CTCGAG 1 cut(s) 1104
PcsI WCGNNNNNNNCGW 1 cut(s) 1283
PctI GAATGC 1 cut(s) 835
PdiI GCCGGC 1 cut(s) 997
PdmI GAANNNNTTC 1 cut(s) 1342
PfeI GAWTC 4 cut(s) 724, 776, 1423, 1456
PkrI GCNGC 4 cut(s) 865, 1264, 1357, 1520
Ple19I CGATCG 1 cut(s) 269
PleI GAGTC 2 cut(s) 715, 1064
PluTI GGCGCC 1 cut(s) 908
PpsI GAGTC 2 cut(s) 715, 1064
Psp124BI GAGCTC 2 cut(s) 200, 548
Psp6I CCWGG 1 cut(s) 895
PspEI GGTNACC 1 cut(s) 891
PspFI CCCAGC 1 cut(s) 1022
PspGI CCWGG 1 cut(s) 895
PspN4I GGNNCC 8 cut(s) 175, 219, 232, 241, 630, 906, 1028, 1143
PspOMI GGGCCC 1 cut(s) 173
PspPI GGNCC 7 cut(s) 173, 174, 230, 474, 618, 1232, 1364
PspXI VCTCGAGB 1 cut(s) 1104
PstI CTGCAG 2 cut(s) 814, 868
PstNI CAGNNNCTG 2 cut(s) 809, 1346
PvuI CGATCG 1 cut(s) 269
PvuII CAGCTG 1 cut(s) 989
RsaI GTAC 3 cut(s) 299, 425, 1276
RsaNI GTAC 3 cut(s) 298, 424, 1275
RseI CAYNNNNRTG 1 cut(s) 315
SacI GAGCTC 2 cut(s) 200, 548
SaqAI TTAA 4 cut(s) 374, 507, 744, 1479
SatI GCNGC 4 cut(s) 864, 1263, 1356, 1519
Sau3AI GATC 8 cut(s) 5, 262, 266, 319, 528, 800, 1267, 1287
Sau96I GGNCC 7 cut(s) 173, 174, 230, 474, 618, 1232, 1364
SchI GAGTC 2 cut(s) 716, 1065
ScrFI CCNGG 6 cut(s) 235, 236, 633, 634, 897, 1378
SduI GDGCHC 6 cut(s) 177, 200, 220, 242, 548, 633
SexAI ACCWGGT 1 cut(s) 895
SfaNI GCATC 4 cut(s) 596, 826, 832, 1530
SfcI CTRYAG 3 cut(s) 489, 810, 864
SfoI GGCGCC 1 cut(s) 906
Sfr274I CTCGAG 1 cut(s) 1104
SfuI TTCGAA 1 cut(s) 932
SinI GGWCC 2 cut(s) 230, 618
SlaI CTCGAG 1 cut(s) 1104
SmaI CCCGGG 2 cut(s) 236, 634
SmiMI CAYNNNNRTG 1 cut(s) 315
SmlI CTYRAG 1 cut(s) 1104
SmoI CTYRAG 1 cut(s) 1104
SphI GCATGC 1 cut(s) 1003
Sse9I AATT 8 cut(s) 37, 87, 190, 602, 757, 1068, 1297, 1487
SsiI CCGC 3 cut(s) 434, 1367, 1519
SspDI GGCGCC 1 cut(s) 904
SspMI CTAG 5 cut(s) 17, 23, 74, 251, 878
SstI GAGCTC 2 cut(s) 200, 548
StyD4I CCNGG 6 cut(s) 233, 234, 631, 632, 895, 1376
TaaI ACNGT 3 cut(s) 181, 1318, 1536
TaiI ACGT 1 cut(s) 1410
TaqI TCGA 8 cut(s) 194, 265, 287, 464, 932, 1044, 1105, 1181
TasI AATT 8 cut(s) 37, 87, 190, 602, 757, 1068, 1297, 1487
TatI WGTACW 1 cut(s) 297
TauI GCSGC 1 cut(s) 1521
TfiI GAWTC 4 cut(s) 724, 776, 1423, 1456
Tru1I TTAA 4 cut(s) 374, 507, 744, 1479
Tru9I TTAA 4 cut(s) 374, 507, 744, 1479
TscAI CASTG 1 cut(s) 1321
TseFI GTSAC 1 cut(s) 1008
TseI GCWGC 3 cut(s) 863, 1262, 1355
Tsp45I GTSAC 1 cut(s) 1008
TspDTI ATGAA 2 cut(s) 401, 746
TspGWI ACGGA 2 cut(s) 190, 1350
TspMI CCCGGG 2 cut(s) 234, 632
TspRI CASTG 1 cut(s) 1321
VpaK11BI GGWCC 2 cut(s) 230, 618
XapI RAATTY 5 cut(s) 87, 602, 757, 1068, 1297
XbaI TCTAGA 1 cut(s) 877
XceI RCATGY 2 cut(s) 314, 1003
XhoI CTCGAG 1 cut(s) 1104
XmaI CCCGGG 2 cut(s) 234, 632
XmiI GTMKAC 1 cut(s) 1382
XmnI GAANNNNTTC 1 cut(s) 1342
XspI CTAG 5 cut(s) 17, 23, 74, 251, 878
Zsp2I ATGCAT 1 cut(s) 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.