pycom04g04510

glycerol-3-phosphate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001726
Physical Location & Seq
Reverse (-)
4106566 .. 4107390
825 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g04510.1

Sequence Viewer

Length: 444 bp
ATGTCATTTGCAATTCCGGCAATGCTATTATATCGAAAATATGGCAGCATTTCAAACAATGTCAACATTACACTTATGATGGTTGCTGGCTTGTTTATTAATGGACCCTATGCACTTATCACCACCGCAGTTTCTGCAGACCTTGGAACGCACAGTTCCCTTAGAGGGGATTCTCGAGCACTGGCAACAGTTACCGCCATAATTGATGGTACTGGATCAGTTGGTGCAGCTTTTGGCCCTCTTCTTTCTGGTTTCCTTTCAACGAAGGAATGGGATGCAGTATTTCTGATGTTAATGATTTGTGCTCTTATTGCGACGCTTCTTTTATCACGTTTGGTTATAGCTGAAATTTCTGAGAAGATGTGCAAACCAGTACCTGTATCCAGCGGGCAGCAAAGTTTTGGAGCTCCTGCATCACAGCCACTTCTAAGAGATCAACGGTGA

Protein Analysis

148

Amino Acids

15.5

Weight (kDa)

8.71

Isoelectric Point (pI)

36.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 21 - 113 6.1e-07 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 126, 195, 387
AclWI GGATC 1 cut(s) 223
AcsI RAATTY 1 cut(s) 348
AfaI GTAC 2 cut(s) 211, 375
AfiI CCNNNNNNNGG 2 cut(s) 165, 166
AgsI TTSAA 2 cut(s) 54, 261
AluBI AGCT 3 cut(s) 230, 344, 407
AluI AGCT 3 cut(s) 230, 344, 407
Alw21I GWGCWC 3 cut(s) 181, 307, 409
AlwI GGATC 1 cut(s) 223
AlwNI CAGNNNCTG 2 cut(s) 134, 377
Ama87I CYCGRG 1 cut(s) 174
AoxI GGCC 1 cut(s) 235
ApeKI GCWGC 3 cut(s) 45, 227, 391
ApoI RAATTY 1 cut(s) 348
AseI ATTAAT 1 cut(s) 99
AspS9I GGNCC 2 cut(s) 104, 236
AsuHPI GGTGA 1 cut(s) 112
AvaI CYCGRG 1 cut(s) 174
AvaII GGWCC 1 cut(s) 104
BanII GRGCYC 1 cut(s) 409
Bbv12I GWGCWC 3 cut(s) 181, 307, 409
BbvI GCAGC 3 cut(s) 57, 239, 403
BccI CCATC 2 cut(s) 73, 200
BciVI GTATCC 1 cut(s) 391
BfmI CTRYAG 1 cut(s) 135
BfuI GTATCC 1 cut(s) 391
BisI GCNGC 3 cut(s) 46, 228, 392
BlsI GCNGC 3 cut(s) 47, 229, 393
Bme18I GGWCC 1 cut(s) 104
BmeT110I CYCGRG 1 cut(s) 174
BmgT120I GGNCC 2 cut(s) 104, 236
BmiI GGNNCC 1 cut(s) 106
BmsI GCATC 2 cut(s) 265, 422
BsaJI CCNNGG 1 cut(s) 142
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 166
Bse1I ACTGG 3 cut(s) 186, 217, 371
Bse3DI GCAATG 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 1 cut(s) 280
BseLI CCNNNNNNNGG 2 cut(s) 165, 166
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 3 cut(s) 186, 217, 371
BseXI GCAGC 3 cut(s) 57, 239, 403
BsgI GTGCAG 1 cut(s) 246
BshFI GGCC 1 cut(s) 237
BsiHKAI GWGCWC 3 cut(s) 181, 307, 409
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 1 cut(s) 17
BslI CCNNNNNNNGG 2 cut(s) 165, 166
BsnI GGCC 1 cut(s) 237
BsoBI CYCGRG 1 cut(s) 174
Bsp1286I GDGCHC 3 cut(s) 181, 307, 409
Bsp143I GATC 2 cut(s) 215, 433
BspACI CCGC 3 cut(s) 126, 195, 387
BspANI GGCC 1 cut(s) 237
BspCNI CTCAG 1 cut(s) 346
BspLI GGNNCC 1 cut(s) 106
BspMAI CTGCAG 1 cut(s) 139
BspPI GGATC 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 27
BsrI ACTGG 3 cut(s) 186, 217, 371
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 2 cut(s) 215, 433
BssT1I CCWWGG 1 cut(s) 142
Bst4CI ACNGT 3 cut(s) 155, 190, 441
Bst6I CTCTTC 1 cut(s) 246
BstAPI GCANNNNNTGC 1 cut(s) 134
BstC8I GCNNGC 2 cut(s) 88, 389
BstDEI CTNAG 3 cut(s) 161, 354, 428
BstF5I GGATG 1 cut(s) 280
BstKTI GATC 2 cut(s) 218, 436
BstMBI GATC 2 cut(s) 215, 433
BstMWI GCNNNNNNNGC 3 cut(s) 17, 134, 311
BstSFI CTRYAG 1 cut(s) 135
BstV1I GCAGC 3 cut(s) 57, 239, 403
BsuI GTATCC 1 cut(s) 391
BsuRI GGCC 1 cut(s) 237
BtsCI GGATG 1 cut(s) 280
BtsIMutI CAGTG 1 cut(s) 179
Cac8I GCNNGC 2 cut(s) 88, 389
CaiI CAGNNNCTG 2 cut(s) 134, 377
Cfr13I GGNCC 2 cut(s) 104, 236
CseI GACGC 1 cut(s) 325
Csp6I GTAC 2 cut(s) 210, 374
CviJI RGCY 6 cut(s) 90, 230, 237, 344, 407, 421
CviKI_1 RGCY 6 cut(s) 90, 230, 237, 344, 407, 421
CviQI GTAC 2 cut(s) 210, 374
DdeI CTNAG 3 cut(s) 161, 354, 428
DpnI GATC 2 cut(s) 217, 435
DpnII GATC 2 cut(s) 215, 433
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Ecl136II GAGCTC 1 cut(s) 407
Eco130I CCWWGG 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 104
Eco53kI GAGCTC 1 cut(s) 407
Eco88I CYCGRG 1 cut(s) 174
EcoICRI GAGCTC 1 cut(s) 407
EcoT14I CCWWGG 1 cut(s) 142
EcoT38I GRGCYC 1 cut(s) 409
ErhI CCWWGG 1 cut(s) 142
FaiI YATR 6 cut(s) 31, 42, 77, 111, 200, 341
FauI CCCGC 1 cut(s) 380
Fnu4HI GCNGC 3 cut(s) 46, 228, 392
FokI GGATG 1 cut(s) 287
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 3 cut(s) 46, 228, 392
GluI GCNGC 3 cut(s) 46, 228, 392
HaeIII GGCC 1 cut(s) 237
HapII CCGG 1 cut(s) 17
HgaI GACGC 1 cut(s) 325
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HinfI GANTC 1 cut(s) 170
HpaII CCGG 1 cut(s) 17
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 2 cut(s) 288, 355
Hpy188III TCNNGA 1 cut(s) 174
Hpy8I GTNNAC 1 cut(s) 64
Hpy99I CGWCG 1 cut(s) 319
HpyAV CCTTC 1 cut(s) 259
HpyCH4III ACNGT 3 cut(s) 155, 190, 441
HpyCH4IV ACGT 1 cut(s) 331
HpyCH4V TGCA 7 cut(s) 11, 113, 137, 227, 278, 366, 413
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 134, 311
HpyF3I CTNAG 3 cut(s) 161, 354, 428
HpySE526I ACGT 1 cut(s) 331
Kzo9I GATC 2 cut(s) 215, 433
LmnI GCTCC 2 cut(s) 404, 412
LpnPI CCDG 9 cut(s) 30, 72, 167, 198, 234, 384, 390, 397, 423
Lsp1109I GCAGC 3 cut(s) 57, 239, 403
LweI GCATC 2 cut(s) 265, 422
MaeII ACGT 1 cut(s) 331
MaeIII GTNAC 1 cut(s) 190
MalI GATC 2 cut(s) 217, 435
MboI GATC 2 cut(s) 215, 433
MboII GAAGA 2 cut(s) 233, 370
MhlI GDGCHC 3 cut(s) 181, 307, 409
MluCI AATT 3 cut(s) 12, 201, 348
MnlI CCTC 2 cut(s) 158, 249
MseI TTAA 2 cut(s) 99, 293
MspA1I CMGCKG 1 cut(s) 387
MspI CCGG 1 cut(s) 17
MwoI GCNNNNNNNGC 3 cut(s) 17, 134, 311
NdeII GATC 2 cut(s) 215, 433
NlaIV GGNNCC 1 cut(s) 106
PaeR7I CTCGAG 1 cut(s) 174
PfeI GAWTC 1 cut(s) 170
PkrI GCNGC 3 cut(s) 47, 229, 393
PshBI ATTAAT 1 cut(s) 99
Psp124BI GAGCTC 1 cut(s) 409
PspN4I GGNNCC 1 cut(s) 106
PspPI GGNCC 2 cut(s) 104, 236
PstI CTGCAG 1 cut(s) 139
PstNI CAGNNNCTG 2 cut(s) 134, 377
RsaI GTAC 2 cut(s) 211, 375
RsaNI GTAC 2 cut(s) 210, 374
SacI GAGCTC 1 cut(s) 409
SaqAI TTAA 2 cut(s) 99, 293
SatI GCNGC 3 cut(s) 46, 228, 392
Sau3AI GATC 2 cut(s) 215, 433
Sau96I GGNCC 2 cut(s) 104, 236
SduI GDGCHC 3 cut(s) 181, 307, 409
SetI ASST 6 cut(s) 144, 232, 334, 346, 379, 409
SfaNI GCATC 2 cut(s) 265, 422
SfcI CTRYAG 1 cut(s) 135
Sfr274I CTCGAG 1 cut(s) 174
SinI GGWCC 1 cut(s) 104
SlaI CTCGAG 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 174
SmoI CTYRAG 1 cut(s) 174
Sse9I AATT 3 cut(s) 12, 201, 348
SsiI CCGC 3 cut(s) 126, 195, 387
SstI GAGCTC 1 cut(s) 409
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 3 cut(s) 155, 190, 441
TaiI ACGT 1 cut(s) 334
TaqI TCGA 2 cut(s) 34, 175
TasI AATT 3 cut(s) 12, 201, 348
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 2 cut(s) 99, 293
Tru9I TTAA 2 cut(s) 99, 293
TscAI CASTG 1 cut(s) 186
TseI GCWGC 3 cut(s) 45, 227, 391
TspRI CASTG 1 cut(s) 186
VpaK11BI GGWCC 1 cut(s) 104
VspI ATTAAT 1 cut(s) 99
XapI RAATTY 1 cut(s) 348
XhoI CTCGAG 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.