pycom02g07220

Leucine-rich repeats, outliers

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002012
Physical Location & Seq
Forward (+)
5053289 .. 5054735
1447 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g07220.1

Sequence Viewer

Length: 675 bp
ATGCTTATGCTACCAGAACTGAAGAAGCTTGAGTGTTTGGAGGTTTTCTCGCTAGCAGGCTTCGAAAATGTTTGTGACAATTTTATTAGGGAATTTATCACTGCTTGTGGTCACAGTTTGAAGGAGCTTGTTCTGACTGATTGTGTGAAATTAACAGATTCTTCTGTGAAAGTGATAGCTGAAAGCTGTTCGGGATTATGTGCACTTGACCTTGCTAACTTGAACAAATTGACTGATTCTACTTTAGGATATCTTGCAAATGGTTGCCGAGCAATTCAAACATTAAATTTTCGACGGAATCCATTCAGTGATGAAGCCATTGCTGCATTTTTGGAAACTTCTGGAGAATGCTTACAGGAACTTTCACTAAATCATATCGAGATGGTCGGCCACAACACAGCCATATCACTTGCCAAACGTTCAAGGATGTTGCATACTCTAGATCTATCATGGTGCCGGAATTTGACAGATGAGGCCCTCGGTTTAATTGTGGACAGCTGCCTATCACTGAGGATACTAAAACTTCTTGGATGCACTCAGATCACAGATACTTTTCTGGATGGACACTCAAATCCAGAGGTGAGAATTATTGGCTTGAAATTTTCTCCGATACTAGAACATCTCAAGGCGCTTAATCCTCACGAAGGGCCATTGCGTTATTCCTCAGTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

225

Amino Acids

24.73

Weight (kDa)

5.49

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 50 - 198 2e-08 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 453
AclI AACGTT 1 cut(s) 418
AcoI YGGCCR 1 cut(s) 388
AcsI RAATTY 4 cut(s) 92, 286, 460, 599
AcuI CTGAAG 1 cut(s) 41
AfiI CCNNNNNNNGG 1 cut(s) 644
AgsI TTSAA 5 cut(s) 121, 223, 278, 423, 598
AluBI AGCT 5 cut(s) 28, 127, 179, 186, 498
AluI AGCT 5 cut(s) 28, 127, 179, 186, 498
Alw21I GWGCWC 1 cut(s) 205
Alw44I GTGCAC 1 cut(s) 201
AoxI GGCC 3 cut(s) 388, 474, 647
ApaLI GTGCAC 1 cut(s) 201
ApeKI GCWGC 2 cut(s) 323, 498
ApoI RAATTY 4 cut(s) 92, 286, 460, 599
Asp700I GAANNNNTTC 1 cut(s) 302
AspLEI GCGC 1 cut(s) 631
AspS9I GGNCC 2 cut(s) 475, 647
AsuHPI GGTGA 1 cut(s) 592
AsuII TTCGAA 1 cut(s) 63
AsuNHI GCTAGC 1 cut(s) 52
BaeGI GKGCMC 1 cut(s) 205
BanI GGYRCC 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 205
BbvI GCAGC 2 cut(s) 310, 485
BccI CCATC 2 cut(s) 376, 554
BciVI GTATCC 1 cut(s) 507
BfaI CTAG 3 cut(s) 53, 440, 614
BfoI RGCGCY 1 cut(s) 632
BfuI GTATCC 1 cut(s) 507
BglII AGATCT 1 cut(s) 442
BisI GCNGC 2 cut(s) 324, 499
BlsI GCNGC 2 cut(s) 325, 500
BmgT120I GGNCC 2 cut(s) 475, 647
BmiI GGNNCC 1 cut(s) 455
BmsI GCATC 1 cut(s) 521
BmtI GCTAGC 1 cut(s) 56
BplI GAGNNNNNCTC 2 cut(s) 32, 64
BpmI CTGGAG 1 cut(s) 363
Bpu14I TTCGAA 1 cut(s) 63
BpuEI CTTGAG 2 cut(s) 50, 608
BsaJI CCNNGG 1 cut(s) 478
Bsc4I CCNNNNNNNGG 1 cut(s) 644
Bse3DI GCAATG 2 cut(s) 318, 650
BseDI CCNNGG 1 cut(s) 478
BseGI GGATG 3 cut(s) 432, 536, 565
BseLI CCNNNNNNNGG 1 cut(s) 644
BseMI GCAATG 2 cut(s) 318, 650
BseMII CTCAG 2 cut(s) 500, 551
BseSI GKGCMC 1 cut(s) 205
BseXI GCAGC 2 cut(s) 310, 485
BshFI GGCC 3 cut(s) 390, 476, 649
BshNI GGYRCC 1 cut(s) 453
BsiHKAI GWGCWC 1 cut(s) 205
BsiSI CCGG 1 cut(s) 457
BslI CCNNNNNNNGG 1 cut(s) 644
BsmI GAATGC 1 cut(s) 353
BsnI GGCC 3 cut(s) 390, 476, 649
Bsp119I TTCGAA 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 205
Bsp143I GATC 2 cut(s) 442, 540
BspANI GGCC 3 cut(s) 390, 476, 649
BspCNI CTCAG 2 cut(s) 501, 550
BspLI GGNNCC 1 cut(s) 455
BspOI GCTAGC 1 cut(s) 56
BspT104I TTCGAA 1 cut(s) 63
BspT107I GGYRCC 1 cut(s) 453
BsrDI GCAATG 2 cut(s) 318, 650
BssECI CCNNGG 1 cut(s) 478
BssMI GATC 2 cut(s) 442, 540
Bst4CI ACNGT 1 cut(s) 116
BstBI TTCGAA 1 cut(s) 63
BstC8I GCNNGC 2 cut(s) 54, 58
BstDEI CTNAG 3 cut(s) 509, 537, 664
BstENI CCTNNNNNAGG 1 cut(s) 642
BstF5I GGATG 3 cut(s) 432, 536, 565
BstH2I RGCGCY 1 cut(s) 632
BstHHI GCGC 1 cut(s) 631
BstKTI GATC 2 cut(s) 445, 543
BstMBI GATC 2 cut(s) 442, 540
BstMWI GCNNNNNNNGC 1 cut(s) 323
BstSLI GKGCMC 1 cut(s) 205
BstV1I GCAGC 2 cut(s) 310, 485
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BsuI GTATCC 1 cut(s) 507
BsuRI GGCC 3 cut(s) 390, 476, 649
BtsCI GGATG 3 cut(s) 432, 536, 565
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 3 cut(s) 99, 313, 506
Cac8I GCNNGC 2 cut(s) 54, 58
CfoI GCGC 1 cut(s) 631
Cfr13I GGNCC 2 cut(s) 475, 647
CviAII CATG 1 cut(s) 450
DdeI CTNAG 3 cut(s) 509, 537, 664
DpnI GATC 2 cut(s) 444, 542
DpnII GATC 2 cut(s) 442, 540
EaeI YGGCCR 1 cut(s) 388
Eco32I GATATC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 41
EcoNI CCTNNNNNAGG 1 cut(s) 642
EcoO109I RGGNCCY 1 cut(s) 475
EcoRV GATATC 1 cut(s) 251
FaeI CATG 1 cut(s) 453
FaiI YATR 6 cut(s) 8, 199, 375, 404, 435, 451
FatI CATG 1 cut(s) 449
Fnu4HI GCNGC 2 cut(s) 324, 499
FokI GGATG 3 cut(s) 439, 543, 572
Fsp4HI GCNGC 2 cut(s) 324, 499
FspBI CTAG 3 cut(s) 53, 440, 614
GlaI GCGC 1 cut(s) 630
GluI GCNGC 2 cut(s) 324, 499
GsuI CTGGAG 1 cut(s) 363
HaeII RGCGCY 1 cut(s) 632
HaeIII GGCC 3 cut(s) 390, 476, 649
HapII CCGG 1 cut(s) 457
HhaI GCGC 1 cut(s) 631
Hin1II CATG 1 cut(s) 453
Hin6I GCGC 1 cut(s) 629
HinP1I GCGC 1 cut(s) 629
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 3 cut(s) 158, 236, 298
HpaII CCGG 1 cut(s) 457
HphI GGTGA 1 cut(s) 592
Hpy166II GTNNAC 2 cut(s) 203, 493
Hpy188I TCNGA 3 cut(s) 135, 540, 609
Hpy188III TCNNGA 7 cut(s) 192, 342, 379, 440, 557, 575, 641
Hpy8I GTNNAC 2 cut(s) 203, 493
Hpy99I CGWCG 1 cut(s) 297
HpyAV CCTTC 2 cut(s) 115, 638
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 1 cut(s) 418
HpyCH4V TGCA 5 cut(s) 203, 257, 326, 433, 534
HpyF10VI GCNNNNNNNGC 1 cut(s) 323
HpyF3I CTNAG 3 cut(s) 509, 537, 664
HpySE526I ACGT 1 cut(s) 418
Hsp92II CATG 1 cut(s) 453
HspAI GCGC 1 cut(s) 629
Kzo9I GATC 2 cut(s) 442, 540
LmnI GCTCC 1 cut(s) 124
LpnPI CCDG 7 cut(s) 27, 42, 327, 341, 470, 542, 588
Lsp1109I GCAGC 2 cut(s) 310, 485
LweI GCATC 1 cut(s) 521
MaeI CTAG 3 cut(s) 53, 440, 614
MaeII ACGT 1 cut(s) 418
MaeIII GTNAC 2 cut(s) 74, 110
MalI GATC 2 cut(s) 444, 542
MboI GATC 2 cut(s) 442, 540
MboII GAAGA 2 cut(s) 34, 153
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 1 cut(s) 205
MnlI CCTC 7 cut(s) 34, 466, 488, 504, 571, 648, 673
MroXI GAANNNNTTC 1 cut(s) 302
MseI TTAA 4 cut(s) 152, 284, 485, 633
MspA1I CMGCKG 1 cut(s) 498
MspI CCGG 1 cut(s) 457
Mva1269I GAATGC 1 cut(s) 353
MwoI GCNNNNNNNGC 1 cut(s) 323
NdeII GATC 2 cut(s) 442, 540
NheI GCTAGC 1 cut(s) 52
NlaIII CATG 1 cut(s) 453
NlaIV GGNNCC 1 cut(s) 455
NmeAIII GCCGAG 1 cut(s) 293
NmuCI GTSAC 2 cut(s) 74, 110
NspV TTCGAA 1 cut(s) 63
PctI GAATGC 1 cut(s) 353
PdmI GAANNNNTTC 1 cut(s) 302
PfeI GAWTC 3 cut(s) 158, 236, 298
PkrI GCNGC 2 cut(s) 325, 500
Psp1406I AACGTT 1 cut(s) 418
PspN4I GGNNCC 1 cut(s) 455
PspPI GGNCC 2 cut(s) 475, 647
PsuI RGATCY 1 cut(s) 442
PvuII CAGCTG 1 cut(s) 498
SaqAI TTAA 4 cut(s) 152, 284, 485, 633
SatI GCNGC 2 cut(s) 324, 499
Sau3AI GATC 2 cut(s) 442, 540
Sau96I GGNCC 2 cut(s) 475, 647
SduI GDGCHC 1 cut(s) 205
SetI ASST 9 cut(s) 30, 45, 129, 181, 188, 213, 421, 500, 582
SfaNI GCATC 1 cut(s) 521
SfuI TTCGAA 1 cut(s) 63
SmlI CTYRAG 2 cut(s) 29, 623
SmoI CTYRAG 2 cut(s) 29, 623
SspMI CTAG 3 cut(s) 53, 440, 614
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 1 cut(s) 421
TaqI TCGA 3 cut(s) 63, 292, 378
TfiI GAWTC 3 cut(s) 158, 236, 298
Tru1I TTAA 4 cut(s) 152, 284, 485, 633
Tru9I TTAA 4 cut(s) 152, 284, 485, 633
TscAI CASTG 3 cut(s) 106, 313, 513
TseFI GTSAC 2 cut(s) 74, 110
TseI GCWGC 2 cut(s) 323, 498
Tsp45I GTSAC 2 cut(s) 74, 110
TspDTI ATGAA 1 cut(s) 327
TspGWI ACGGA 1 cut(s) 310
TspRI CASTG 3 cut(s) 106, 313, 513
VneI GTGCAC 1 cut(s) 201
XagI CCTNNNNNAGG 1 cut(s) 642
XapI RAATTY 4 cut(s) 92, 286, 460, 599
XbaI TCTAGA 1 cut(s) 439
XmnI GAANNNNTTC 1 cut(s) 302
XspI CTAG 3 cut(s) 53, 440, 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.