pycom15g19230

Leucine-rich repeats, outliers

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002012
Physical Location & Seq
Forward (+)
13702473 .. 13705472
3000 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g19230.1

Sequence Viewer

Length: 1482 bp
ATGGAGCGGTTTCGCGAAACAGCTAGGCGAAATGCACGTCGATTTGCATACTTTTCTGCTGAAGAGGAACAGGTGAATCATTTGCCTCCTGATGCTGAAGTAGCACGGGATATCGAAGATTGGCCTGGTCCCTTCTCTACGGCCATGAAAATTATTAAGGATCGAGCAGAAAAAGATGTGCAAGTGCCCTCTAACAAGACCAAACAATCATCAGTGAATTGGGTTCCTAAGAAGTTTCCAGATCGTCCCCTCCCTAAGATTTCGGTCCCCTCACTGCAAGATCTGTGTTTGGTGGTTCTCGCTAAGAATGCTGATGCAATTGTTTCACTTGACCACGTGGCAGATGCTTTGAGGCACCGGCTAAGTCAGATGCTTTGTGATTCTCGAAAAATGAACAGTCATTTATTCGAACTTTTTGTTCAGGGATCTCCAACAGAGGTTCGGTTACGGGATTGCTCATGGATGACAGAGGAGCAGTTTACAGACTCTTTTCAGCAGTGCGACACCACCAATTTAACAGTGCTACAACTTGATCAGTGTGGGCGCTGTATGCCAGATTATATATTGCATTCTACTTTAGCTCAGTCATCAAATTGCTTGCCCAACCTAGTCACTTTATCCCTAAGTGGTGCATGCCGTCTTTCGGATATTGGGCTTGGTAAAGTTATATCTTCTGCTCCGGCACTAAGATCTCTAAACCTCAGCCAGTGCTCTCTTCTCACTTACTCAAGTATTGGCACTTTAGCTGACTCATTGGGATCAGTTCTGAAGGAACTTTATCTAAATGATTGCCAGGGCATTGATGCTATGCTTATGCTACCAGCACTCAAGAAGCTTGAGCGTTTGGAGGTTTTTTCGCTAGCAGGCTTTGAAAATGTTTATGACAGTTTTATTAGGGAGTTTATTACAGCTCGTGGTCACAGTTTGAAGGAGCTTGTTCTGACTGATTGTGTACAATTAACAGATTCTTCTGTGAAAGTGATAGCTGAAACCTGTTCGGGATTATGTGCGCTTGACCTTGCTAACTTGAACAAATTGACTGATTCTACTTTAGGATATCTTGCAAATGGTTGCCGAGCAATTCAAACATTAAATTTTCATCGGAATCCATTCAGTGATGAAGCCATTGCTGCATTCCTGGAAACTTCTGGAGAATGCTTACAGGAACTTTCACTAAATCATATCCAGATGGTCGGCCACAACACAGCCATATCACTTGCCAAACGATCAAGGATGTTGCATACCCTAGATCTATCATGGTGCCGGAATATTACAGACGAGGCCCTGGGTTTAATCGTGGACAGCTGCCTATCACTGAGAATACTAAAACTTGTCGGATGCACTCAGATCACAGATACTTTTCTGGATGGACACTCAAATCCAGAGGTGAGAATCATTGGCATGAAATTTTCTCCGATACTAGAACATCTCAAGGTCCCCAATCCTCACGAAGGTCCATTGCGTTATTCCGCCGTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

494

Amino Acids

54.79

Weight (kDa)

5.84

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 319 - 459 1.7e-08 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 354, 1260
AccBSI CCGCTC 1 cut(s) 7
AccII CGCG 1 cut(s) 15
AciI CCGC 2 cut(s) 7, 1470
AclWI GGATC 3 cut(s) 168, 433, 766
AcoI YGGCCR 2 cut(s) 141, 1195
AcsI RAATTY 2 cut(s) 1093, 1406
AcuI CTGAAG 3 cut(s) 81, 117, 788
AcvI CACGTG 1 cut(s) 337
AfaI GTAC 1 cut(s) 954
AfiI CCNNNNNNNGG 2 cut(s) 643, 1451
AgsI TTSAA 4 cut(s) 872, 928, 1030, 1085
AjiI CACGTC 1 cut(s) 38
AjnI CCWGG 4 cut(s) 124, 792, 1137, 1284
AluBI AGCT 8 cut(s) 23, 581, 746, 835, 911, 934, 986, 1305
AluI AGCT 8 cut(s) 23, 581, 746, 835, 911, 934, 986, 1305
Alw21I GWGCWC 1 cut(s) 713
AlwI GGATC 3 cut(s) 168, 433, 766
AoxI GGCC 4 cut(s) 122, 141, 1195, 1281
ApeKI GCWGC 2 cut(s) 1130, 1305
ApoI RAATTY 2 cut(s) 1093, 1406
ArsI GACNNNNNNTTYG 2 cut(s) 22, 54
Asp700I GAANNNNTTC 1 cut(s) 1109
AspLEI GCGC 2 cut(s) 546, 1012
AspS9I GGNCC 5 cut(s) 128, 265, 1282, 1435, 1454
AsuHPI GGTGA 2 cut(s) 85, 1399
AsuII TTCGAA 1 cut(s) 408
AsuNHI GCTAGC 1 cut(s) 859
AvaII GGWCC 4 cut(s) 128, 265, 1435, 1454
BaeGI GKGCMC 1 cut(s) 189
BanI GGYRCC 2 cut(s) 354, 1260
BauI CACGAG 1 cut(s) 912
BbrPI CACGTG 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 713
BbvCI CCTCAGC 1 cut(s) 701
BbvI GCAGC 2 cut(s) 1117, 1292
BccI CCATC 2 cut(s) 1183, 1361
BceAI ACGGC 3 cut(s) 156, 621, 1457
BciT130I CCWGG 4 cut(s) 126, 794, 1139, 1286
BclI TGATCA 1 cut(s) 532
BfaI CTAG 5 cut(s) 24, 608, 860, 1247, 1421
BfoI RGCGCY 1 cut(s) 547
BglII AGATCT 3 cut(s) 280, 689, 1249
BisI GCNGC 2 cut(s) 1131, 1306
BlsI GCNGC 2 cut(s) 1132, 1307
Bme1390I CCNGG 4 cut(s) 126, 794, 1139, 1286
Bme18I GGWCC 4 cut(s) 128, 265, 1435, 1454
BmgBI CACGTC 1 cut(s) 38
BmgT120I GGNCC 5 cut(s) 128, 265, 1282, 1435, 1454
BmiI GGNNCC 6 cut(s) 130, 225, 267, 356, 1262, 1437
BmrFI CCNGG 4 cut(s) 126, 794, 1139, 1286
BmsI GCATC 6 cut(s) 82, 304, 334, 360, 793, 1328
BmtI GCTAGC 1 cut(s) 863
BpmI CTGGAG 1 cut(s) 1170
Bpu10I CCTNAGC 1 cut(s) 701
Bpu14I TTCGAA 1 cut(s) 408
BpuEI CTTGAG 4 cut(s) 712, 812, 857, 1415
BsaAI YACGTR 1 cut(s) 337
BsaJI CCNNGG 3 cut(s) 793, 1284, 1285
Bsc4I CCNNNNNNNGG 2 cut(s) 643, 1451
Bse118I RCCGGY 1 cut(s) 357
Bse1I ACTGG 1 cut(s) 706
Bse3DI GCAATG 2 cut(s) 1125, 1457
BseBI CCWGG 4 cut(s) 126, 794, 1139, 1286
BseDI CCNNGG 3 cut(s) 793, 1284, 1285
BseGI GGATG 4 cut(s) 468, 1239, 1343, 1372
BseLI CCNNNNNNNGG 2 cut(s) 643, 1451
BseMI GCAATG 2 cut(s) 1125, 1457
BseMII CTCAG 4 cut(s) 596, 715, 1307, 1358
BseNI ACTGG 1 cut(s) 706
BseRI GAGGAG 1 cut(s) 485
BseSI GKGCMC 1 cut(s) 189
BseXI GCAGC 2 cut(s) 1117, 1292
Bsh1236I CGCG 1 cut(s) 15
BshFI GGCC 4 cut(s) 124, 143, 1197, 1283
BshNI GGYRCC 2 cut(s) 354, 1260
BsiHKAI GWGCWC 1 cut(s) 713
BsiSI CCGG 3 cut(s) 358, 680, 1264
BslFI GGGAC 4 cut(s) 114, 231, 251, 1421
BslI CCNNNNNNNGG 2 cut(s) 643, 1451
BsmFI GGGAC 4 cut(s) 114, 231, 251, 1421
BsmI GAATGC 4 cut(s) 313, 568, 1133, 1160
BsnI GGCC 4 cut(s) 124, 143, 1197, 1283
Bsp119I TTCGAA 1 cut(s) 408
Bsp1286I GDGCHC 2 cut(s) 189, 713
Bsp1407I TGTACA 1 cut(s) 952
Bsp68I TCGCGA 1 cut(s) 15
BspACI CCGC 2 cut(s) 7, 1470
BspANI GGCC 4 cut(s) 124, 143, 1197, 1283
BspCNI CTCAG 4 cut(s) 595, 714, 1308, 1357
BspFNI CGCG 1 cut(s) 15
BspLI GGNNCC 6 cut(s) 130, 225, 267, 356, 1262, 1437
BspOI GCTAGC 1 cut(s) 863
BspPI GGATC 3 cut(s) 168, 433, 766
BspT104I TTCGAA 1 cut(s) 408
BspT107I GGYRCC 2 cut(s) 354, 1260
BsrBI CCGCTC 1 cut(s) 7
BsrDI GCAATG 2 cut(s) 1125, 1457
BsrFI RCCGGY 1 cut(s) 357
BsrGI TGTACA 1 cut(s) 952
BsrI ACTGG 1 cut(s) 706
BssAI RCCGGY 1 cut(s) 357
BssECI CCNNGG 3 cut(s) 793, 1284, 1285
BssSI CACGAG 1 cut(s) 912
Bst2BI CACGAG 1 cut(s) 912
Bst2UI CCWGG 4 cut(s) 126, 794, 1139, 1286
Bst4CI ACNGT 4 cut(s) 398, 520, 887, 923
Bst6I CTCTTC 2 cut(s) 57, 720
BstAUI TGTACA 1 cut(s) 952
BstBAI YACGTR 1 cut(s) 337
BstBI TTCGAA 1 cut(s) 408
BstC8I GCNNGC 4 cut(s) 599, 634, 861, 865
BstENI CCTNNNNNAGG 1 cut(s) 1449
BstF5I GGATG 4 cut(s) 468, 1239, 1343, 1372
BstFNI CGCG 1 cut(s) 15
BstH2I RGCGCY 1 cut(s) 547
BstHHI GCGC 2 cut(s) 546, 1012
BstMWI GCNNNNNNNGC 4 cut(s) 101, 308, 550, 1130
BstNI CCWGG 4 cut(s) 126, 794, 1139, 1286
BstNSI RCATGY 1 cut(s) 636
BstSCI CCNGG 4 cut(s) 124, 792, 1137, 1284
BstSLI GKGCMC 1 cut(s) 189
BstUI CGCG 1 cut(s) 15
BstV1I GCAGC 2 cut(s) 1117, 1292
BstX2I RGATCY 4 cut(s) 280, 425, 689, 1249
BstYI RGATCY 4 cut(s) 280, 425, 689, 1249
BsuRI GGCC 4 cut(s) 124, 143, 1197, 1283
BtrI CACGTC 1 cut(s) 38
BtsCI GGATG 4 cut(s) 468, 1239, 1343, 1372
BtsI GCAGTG 2 cut(s) 272, 503
BtsIMutI CAGTG 8 cut(s) 219, 272, 503, 525, 542, 713, 1120, 1313
BtuMI TCGCGA 1 cut(s) 15
Cac8I GCNNGC 4 cut(s) 599, 634, 861, 865
CfoI GCGC 2 cut(s) 546, 1012
Cfr10I RCCGGY 1 cut(s) 357
Cfr13I GGNCC 5 cut(s) 128, 265, 1282, 1435, 1454
Csp6I GTAC 1 cut(s) 953
CviAII CATG 5 cut(s) 145, 459, 633, 1257, 1402
CviQI GTAC 1 cut(s) 953
EaeI YGGCCR 2 cut(s) 141, 1195
Eam1104I CTCTTC 2 cut(s) 57, 720
EarI CTCTTC 2 cut(s) 57, 720
EciI GGCGGA 1 cut(s) 1459
Eco32I GATATC 2 cut(s) 112, 1058
Eco47I GGWCC 4 cut(s) 128, 265, 1435, 1454
Eco57I CTGAAG 3 cut(s) 81, 117, 788
Eco72I CACGTG 1 cut(s) 337
EcoNI CCTNNNNNAGG 1 cut(s) 1449
EcoO109I RGGNCCY 2 cut(s) 1282, 1435
EcoRII CCWGG 4 cut(s) 124, 792, 1137, 1284
EcoRV GATATC 2 cut(s) 112, 1058
FaeI CATG 5 cut(s) 148, 462, 636, 1260, 1405
FaqI GGGAC 4 cut(s) 114, 231, 251, 1421
FatI CATG 5 cut(s) 144, 458, 632, 1256, 1401
FbaI TGATCA 1 cut(s) 532
Fnu4HI GCNGC 2 cut(s) 1131, 1306
FokI GGATG 4 cut(s) 475, 1246, 1350, 1379
Fsp4HI GCNGC 2 cut(s) 1131, 1306
FspBI CTAG 5 cut(s) 24, 608, 860, 1247, 1421
GlaI GCGC 2 cut(s) 545, 1011
GluI GCNGC 2 cut(s) 1131, 1306
GsuI CTGGAG 1 cut(s) 1170
HaeII RGCGCY 1 cut(s) 547
HaeIII GGCC 4 cut(s) 124, 143, 1197, 1283
HapII CCGG 3 cut(s) 358, 680, 1264
HhaI GCGC 2 cut(s) 546, 1012
Hin1II CATG 5 cut(s) 148, 462, 636, 1260, 1405
Hin6I GCGC 2 cut(s) 544, 1010
HinP1I GCGC 2 cut(s) 544, 1010
HindIII AAGCTT 1 cut(s) 833
HinfI GANTC 8 cut(s) 76, 380, 485, 749, 965, 1043, 1105, 1392
HpaII CCGG 3 cut(s) 358, 680, 1264
HphI GGTGA 2 cut(s) 85, 1399
Hpy166II GTNNAC 3 cut(s) 480, 953, 1300
Hpy188I TCNGA 8 cut(s) 369, 646, 768, 942, 1104, 1337, 1347, 1416
Hpy8I GTNNAC 3 cut(s) 480, 953, 1300
Hpy99I CGWCG 1 cut(s) 42
HpyAV CCTTC 4 cut(s) 142, 763, 922, 1445
HpyCH4III ACNGT 4 cut(s) 398, 520, 887, 923
HpyCH4IV ACGT 2 cut(s) 37, 336
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 308, 550, 1130
HpySE526I ACGT 2 cut(s) 37, 336
Hsp92II CATG 5 cut(s) 148, 462, 636, 1260, 1405
HspAI GCGC 2 cut(s) 544, 1010
Ksp22I TGATCA 1 cut(s) 532
LmnI GCTCC 4 cut(s) 4, 472, 682, 931
Lsp1109I GCAGC 2 cut(s) 1117, 1292
LweI GCATC 6 cut(s) 82, 304, 334, 360, 793, 1328
MaeI CTAG 5 cut(s) 24, 608, 860, 1247, 1421
MaeII ACGT 2 cut(s) 37, 336
MaeIII GTNAC 3 cut(s) 444, 610, 917
MbiI CCGCTC 1 cut(s) 7
MboII GAAGA 5 cut(s) 74, 128, 663, 707, 960
MfeI CAATTG 1 cut(s) 318
MflI RGATCY 4 cut(s) 280, 425, 689, 1249
MhlI GDGCHC 2 cut(s) 189, 713
MlyI GAGTC 2 cut(s) 479, 743
MmeI TCCRAC 2 cut(s) 455, 1315
MroXI GAANNNNTTC 1 cut(s) 1109
MseI TTAA 5 cut(s) 156, 515, 959, 1091, 1292
MslI CAYNNNNRTG 1 cut(s) 1400
MspA1I CMGCKG 1 cut(s) 1305
MspI CCGG 3 cut(s) 358, 680, 1264
MspR9I CCNGG 4 cut(s) 126, 794, 1139, 1286
MunI CAATTG 1 cut(s) 318
Mva1269I GAATGC 4 cut(s) 313, 568, 1133, 1160
MvaI CCWGG 4 cut(s) 126, 794, 1139, 1286
MvnI CGCG 1 cut(s) 15
MwoI GCNNNNNNNGC 4 cut(s) 101, 308, 550, 1130
NheI GCTAGC 1 cut(s) 859
NlaIII CATG 5 cut(s) 148, 462, 636, 1260, 1405
NlaIV GGNNCC 6 cut(s) 130, 225, 267, 356, 1262, 1437
NmeAIII GCCGAG 1 cut(s) 1100
NmuCI GTSAC 2 cut(s) 610, 917
NruI TCGCGA 1 cut(s) 15
NspI RCATGY 1 cut(s) 636
NspV TTCGAA 1 cut(s) 408
PaeI GCATGC 1 cut(s) 636
PasI CCCWGGG 1 cut(s) 1285
PctI GAATGC 4 cut(s) 313, 568, 1133, 1160
PdmI GAANNNNTTC 1 cut(s) 1109
PfeI GAWTC 6 cut(s) 76, 380, 965, 1043, 1105, 1392
PfoI TCCNGGA 1 cut(s) 1137
PkrI GCNGC 2 cut(s) 1132, 1307
PleI GAGTC 2 cut(s) 479, 743
PmaCI CACGTG 1 cut(s) 337
PmlI CACGTG 1 cut(s) 337
PpsI GAGTC 2 cut(s) 479, 743
Ppu21I YACGTR 1 cut(s) 337
PpuMI RGGWCCY 1 cut(s) 1435
Psp5II RGGWCCY 1 cut(s) 1435
Psp6I CCWGG 4 cut(s) 124, 792, 1137, 1284
PspCI CACGTG 1 cut(s) 337
PspGI CCWGG 4 cut(s) 124, 792, 1137, 1284
PspN4I GGNNCC 6 cut(s) 130, 225, 267, 356, 1262, 1437
PspPI GGNCC 5 cut(s) 128, 265, 1282, 1435, 1454
PspPPI RGGWCCY 1 cut(s) 1435
PsuI RGATCY 4 cut(s) 280, 425, 689, 1249
PvuII CAGCTG 1 cut(s) 1305
RruI TCGCGA 1 cut(s) 15
RsaI GTAC 1 cut(s) 954
RsaNI GTAC 1 cut(s) 953
RseI CAYNNNNRTG 1 cut(s) 1400
SaqAI TTAA 5 cut(s) 156, 515, 959, 1091, 1292
SatI GCNGC 2 cut(s) 1131, 1306
Sau96I GGNCC 5 cut(s) 128, 265, 1282, 1435, 1454
SchI GAGTC 2 cut(s) 479, 743
ScrFI CCNGG 4 cut(s) 126, 794, 1139, 1286
SduI GDGCHC 2 cut(s) 189, 713
SfaNI GCATC 6 cut(s) 82, 304, 334, 360, 793, 1328
SfuI TTCGAA 1 cut(s) 408
SinI GGWCC 4 cut(s) 128, 265, 1435, 1454
SmiMI CAYNNNNRTG 1 cut(s) 1400
SmlI CTYRAG 4 cut(s) 727, 827, 836, 1430
SmoI CTYRAG 4 cut(s) 727, 827, 836, 1430
SphI GCATGC 1 cut(s) 636
SsiI CCGC 2 cut(s) 7, 1470
SspI AATATT 1 cut(s) 1270
SspMI CTAG 5 cut(s) 24, 608, 860, 1247, 1421
StyD4I CCNGG 4 cut(s) 124, 792, 1137, 1284
TaaI ACNGT 4 cut(s) 398, 520, 887, 923
TaiI ACGT 2 cut(s) 40, 339
TaqI TCGA 5 cut(s) 40, 114, 163, 385, 408
TaqII GACCGA 1 cut(s) 253
TatI WGTACW 1 cut(s) 952
TfiI GAWTC 6 cut(s) 76, 380, 965, 1043, 1105, 1392
Tru1I TTAA 5 cut(s) 156, 515, 959, 1091, 1292
Tru9I TTAA 5 cut(s) 156, 515, 959, 1091, 1292
TscAI CASTG 8 cut(s) 219, 279, 503, 525, 542, 713, 1120, 1320
TseFI GTSAC 2 cut(s) 610, 917
TseI GCWGC 2 cut(s) 1130, 1305
Tsp45I GTSAC 2 cut(s) 610, 917
TspDTI ATGAA 5 cut(s) 161, 407, 1088, 1134, 1418
TspRI CASTG 8 cut(s) 219, 279, 503, 525, 542, 713, 1120, 1320
VpaK11BI GGWCC 4 cut(s) 128, 265, 1435, 1454
XagI CCTNNNNNAGG 1 cut(s) 1449
XapI RAATTY 2 cut(s) 1093, 1406
XceI RCATGY 1 cut(s) 636
XmnI GAANNNNTTC 1 cut(s) 1109
XspI CTAG 5 cut(s) 24, 608, 860, 1247, 1421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.