pycom03g06430

RING-variant domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002651
Physical Location & Seq
Forward (+)
5024274 .. 5026401
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 621 bp
ATGATCCTTTTAACTCCTTTTAAACACTTCTCTGATCGCTGCAGTTTTACCCCCACCATCCTCCTTATAATTCGCCTTGGCTCCCGGACTTCTCTCTCTCTCTCTCTCTCTTCTCCCCACCATGTTCGATATCCAAATGCCGCTACTTTAACACTTCCCTACACCATTTCATTCCATTTCTCCTTCTGGGTTTCACGAATGTCAGCTTCCGAGGCAACCCACCACCGCCGTTCGGCCACCGGAGGCGGCGGCGCGTCGGTCTCGGATGACTCCGACGACGACCAATCTTGGCACTCTATGTCTGAGTCGGGCAATGTCCGGAGGAAGTCTTGTGAGTCGGATTGTCCAGTGGAGGAGGTGGATTTGGAGAGTGGGGTTCTGGAGCTGAAGAAGGTGCTGCATTTGAGTAAAGTTGAGAGAAATTGCAGGATTTGCCACATGGGTTTGGAAGGGGGCGGTGGGACTGACTCTGGCTCTGGGGTCCCAATTGATTTGGGGTGTTCTTGCAAGGGGGATTTGGGCGCTGCTCATAAGCAATGTGCTGAGACTTGGTTCAAGATCAAGGGAGATACTTTCTTTTTTCTTACTTCAATCTGGTCTTGTTTTTCACATGAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

207

Amino Acids

22.56

Weight (kDa)

6.4

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 142 - 190 2.2e-08 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 68
AccII CGCG 1 cut(s) 254
AccIII TCCGGA 1 cut(s) 318
AciI CCGC 5 cut(s) 141, 226, 246, 249, 456
AcoI YGGCCR 1 cut(s) 234
AcuI CTGAAG 1 cut(s) 407
AfiI CCNNNNNNNGG 1 cut(s) 232
AgsI TTSAA 2 cut(s) 556, 591
AluBI AGCT 2 cut(s) 206, 385
AluI AGCT 2 cut(s) 206, 385
Alw26I GTCTC 2 cut(s) 265, 539
Aor13HI TCCGGA 1 cut(s) 318
AoxI GGCC 1 cut(s) 234
ApeKI GCWGC 3 cut(s) 39, 397, 524
AspLEI GCGC 2 cut(s) 254, 524
AspS9I GGNCC 1 cut(s) 481
AsuC2I CCSGG 1 cut(s) 85
AvaII GGWCC 1 cut(s) 481
BbvI GCAGC 3 cut(s) 26, 384, 511
BccI CCATC 1 cut(s) 65
BceAI ACGGC 1 cut(s) 213
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 2 cut(s) 265, 539
BfmI CTRYAG 1 cut(s) 40
BfoI RGCGCY 1 cut(s) 525
BisI GCNGC 6 cut(s) 40, 141, 247, 250, 398, 525
BlsI GCNGC 6 cut(s) 41, 142, 248, 251, 399, 526
Bme1390I CCNGG 1 cut(s) 85
Bme18I GGWCC 1 cut(s) 481
BmgT120I GGNCC 1 cut(s) 481
BmiI GGNNCC 3 cut(s) 82, 482, 483
BmrFI CCNGG 1 cut(s) 85
BpmI CTGGAG 1 cut(s) 401
BpuMI CCSGG 1 cut(s) 85
BsaI GGTCTC 1 cut(s) 265
BsaJI CCNNGG 2 cut(s) 76, 210
BsaWI WCCGGW 2 cut(s) 239, 318
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse1I ACTGG 1 cut(s) 347
Bse3DI GCAATG 2 cut(s) 319, 542
BseAI TCCGGA 1 cut(s) 318
BseDI CCNNGG 2 cut(s) 76, 210
BseGI GGATG 2 cut(s) 57, 271
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMI GCAATG 2 cut(s) 319, 542
BseMII CTCAG 2 cut(s) 294, 534
BseNI ACTGG 1 cut(s) 347
BseRI GAGGAG 1 cut(s) 368
BseXI GCAGC 3 cut(s) 26, 384, 511
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 1 cut(s) 236
BsiSI CCGG 3 cut(s) 85, 240, 319
BslFI GGGAC 2 cut(s) 467, 475
BslI CCNNNNNNNGG 1 cut(s) 232
BsmAI GTCTC 2 cut(s) 265, 539
BsmFI GGGAC 2 cut(s) 467, 475
BsnI GGCC 1 cut(s) 236
Bso31I GGTCTC 1 cut(s) 265
Bsp13I TCCGGA 1 cut(s) 318
Bsp143I GATC 3 cut(s) 3, 34, 558
BspACI CCGC 5 cut(s) 141, 226, 246, 249, 456
BspANI GGCC 1 cut(s) 236
BspCNI CTCAG 2 cut(s) 295, 535
BspEI TCCGGA 1 cut(s) 318
BspFNI CGCG 1 cut(s) 254
BspLI GGNNCC 3 cut(s) 82, 482, 483
BspMAI CTGCAG 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 265
BsrDI GCAATG 2 cut(s) 319, 542
BsrI ACTGG 1 cut(s) 347
BssECI CCNNGG 2 cut(s) 76, 210
BssMI GATC 3 cut(s) 3, 34, 558
BssT1I CCWWGG 1 cut(s) 76
Bst6I CTCTTC 1 cut(s) 115
BstAPI GCANNNNNTGC 1 cut(s) 432
BstDEI CTNAG 2 cut(s) 303, 543
BstF5I GGATG 2 cut(s) 57, 271
BstFNI CGCG 1 cut(s) 254
BstH2I RGCGCY 1 cut(s) 525
BstHHI GCGC 2 cut(s) 254, 524
BstKTI GATC 3 cut(s) 6, 37, 561
BstMAI GTCTC 2 cut(s) 265, 539
BstMBI GATC 3 cut(s) 3, 34, 558
BstMWI GCNNNNNNNGC 2 cut(s) 212, 432
BstSCI CCNGG 1 cut(s) 83
BstSFI CTRYAG 1 cut(s) 40
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 3 cut(s) 26, 384, 511
BsuRI GGCC 1 cut(s) 236
BtsCI GGATG 2 cut(s) 57, 271
BtsIMutI CAGTG 1 cut(s) 354
CfoI GCGC 2 cut(s) 254, 524
Cfr13I GGNCC 1 cut(s) 481
CseI GACGC 1 cut(s) 243
CviAII CATG 3 cut(s) 122, 439, 611
CviJI RGCY 5 cut(s) 81, 206, 236, 385, 474
CviKI_1 RGCY 5 cut(s) 81, 206, 236, 385, 474
DdeI CTNAG 2 cut(s) 303, 543
DpnI GATC 3 cut(s) 5, 36, 560
DpnII GATC 3 cut(s) 3, 34, 558
DraI TTTAAA 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 234
Eam1104I CTCTTC 1 cut(s) 115
EarI CTCTTC 1 cut(s) 115
Eco130I CCWWGG 1 cut(s) 76
Eco31I GGTCTC 1 cut(s) 265
Eco32I GATATC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 481
Eco57I CTGAAG 1 cut(s) 407
EcoO109I RGGNCCY 1 cut(s) 481
EcoRV GATATC 1 cut(s) 131
EcoT14I CCWWGG 1 cut(s) 76
ErhI CCWWGG 1 cut(s) 76
FaeI CATG 3 cut(s) 125, 442, 614
FaiI YATR 6 cut(s) 68, 123, 299, 440, 531, 612
FaqI GGGAC 2 cut(s) 467, 475
FatI CATG 3 cut(s) 121, 438, 610
Fnu4HI GCNGC 6 cut(s) 40, 141, 247, 250, 398, 525
FokI GGATG 2 cut(s) 44, 278
Fsp4HI GCNGC 6 cut(s) 40, 141, 247, 250, 398, 525
GlaI GCGC 2 cut(s) 253, 523
GluI GCNGC 6 cut(s) 40, 141, 247, 250, 398, 525
GsuI CTGGAG 1 cut(s) 401
HaeII RGCGCY 1 cut(s) 525
HaeIII GGCC 1 cut(s) 236
HapII CCGG 3 cut(s) 85, 240, 319
HgaI GACGC 1 cut(s) 243
HhaI GCGC 2 cut(s) 254, 524
Hin1II CATG 3 cut(s) 125, 442, 614
Hin6I GCGC 2 cut(s) 252, 522
HinP1I GCGC 2 cut(s) 252, 522
HinfI GANTC 4 cut(s) 269, 305, 335, 467
HpaII CCGG 3 cut(s) 85, 240, 319
Hpy188I TCNGA 6 cut(s) 34, 211, 265, 274, 304, 340
Hpy188III TCNNGA 4 cut(s) 195, 319, 380, 556
Hpy99I CGWCG 3 cut(s) 259, 278, 281
HpyAV CCTTC 3 cut(s) 193, 385, 443
HpyCH4V TGCA 4 cut(s) 42, 400, 426, 507
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 432
HpyF3I CTNAG 2 cut(s) 303, 543
Hsp92II CATG 3 cut(s) 125, 442, 614
HspAI GCGC 2 cut(s) 252, 522
KflI GGGWCCC 1 cut(s) 481
Kpn2I TCCGGA 1 cut(s) 318
Kzo9I GATC 3 cut(s) 3, 34, 558
LmnI GCTCC 2 cut(s) 86, 382
Lsp1109I GCAGC 3 cut(s) 26, 384, 511
MalI GATC 3 cut(s) 5, 36, 560
MboI GATC 3 cut(s) 3, 34, 558
MboII GAAGA 2 cut(s) 102, 400
MfeI CAATTG 1 cut(s) 486
MluCI AATT 3 cut(s) 69, 421, 486
MlyI GAGTC 4 cut(s) 263, 314, 344, 461
MmeI TCCRAC 2 cut(s) 297, 318
MnlI CCTC 6 cut(s) 71, 205, 236, 315, 346, 349
MroI TCCGGA 1 cut(s) 318
MseI TTAA 4 cut(s) 11, 21, 149, 619
MspI CCGG 3 cut(s) 85, 240, 319
MspR9I CCNGG 1 cut(s) 85
MunI CAATTG 1 cut(s) 486
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 2 cut(s) 212, 432
NciI CCSGG 1 cut(s) 85
NdeII GATC 3 cut(s) 3, 34, 558
NlaIII CATG 3 cut(s) 125, 442, 614
NlaIV GGNNCC 3 cut(s) 82, 482, 483
PfoI TCCNGGA 1 cut(s) 83
PkrI GCNGC 6 cut(s) 41, 142, 248, 251, 399, 526
PleI GAGTC 4 cut(s) 263, 313, 343, 461
PpsI GAGTC 4 cut(s) 263, 313, 343, 461
PpuMI RGGWCCY 1 cut(s) 481
PsiI TTATAA 1 cut(s) 68
Psp5II RGGWCCY 1 cut(s) 481
PspN4I GGNNCC 3 cut(s) 82, 482, 483
PspPI GGNCC 1 cut(s) 481
PspPPI RGGWCCY 1 cut(s) 481
PstI CTGCAG 1 cut(s) 44
SaqAI TTAA 4 cut(s) 11, 21, 149, 619
SatI GCNGC 6 cut(s) 40, 141, 247, 250, 398, 525
Sau3AI GATC 3 cut(s) 3, 34, 558
Sau96I GGNCC 1 cut(s) 481
SchI GAGTC 4 cut(s) 263, 314, 344, 461
ScrFI CCNGG 1 cut(s) 85
SetI ASST 4 cut(s) 208, 360, 387, 396
SfcI CTRYAG 1 cut(s) 40
SinI GGWCC 1 cut(s) 481
Sse9I AATT 3 cut(s) 69, 421, 486
SsiI CCGC 5 cut(s) 141, 226, 246, 249, 456
StyD4I CCNGG 1 cut(s) 83
StyI CCWWGG 1 cut(s) 76
TaqI TCGA 1 cut(s) 127
TaqII GACCGA 1 cut(s) 247
TasI AATT 3 cut(s) 69, 421, 486
TauI GCSGC 3 cut(s) 143, 249, 252
Tru1I TTAA 4 cut(s) 11, 21, 149, 619
Tru9I TTAA 4 cut(s) 11, 21, 149, 619
TscAI CASTG 1 cut(s) 354
TseI GCWGC 3 cut(s) 39, 397, 524
TspDTI ATGAA 1 cut(s) 159
TspRI CASTG 1 cut(s) 354
VpaK11BI GGWCC 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.