pycom03g08250

Protein of unknown function (DUF1635)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002977
Physical Location & Seq
Forward (+)
6666129 .. 6668002
1874 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 609 bp
ATGTGGGTTCTCTCATTTTTCTTCTCCCTTCTCTTCCCTTCTTCCATTTTCTCACCAGAGGTTGAAAGTCTTCCAATGGAGGAACTGGGTTCTCTGTGGACTACTGATCCTGAGAGCGCTGAGGAGCTGAAGCAGAAGCTTCTGTACGCAACCCTTGAGCTAGAATCTGTAAAAACACGCGCAACGGATGAAATCCGGAAACGCGAGGAGAATGTGAAGAAACTAGTCAATCTTCTAAACGTTGCATACAAAGAAAGACAAGATGCCGTGGATCAGCTACAAAAGTTCATAAACAAGATCATGCCTTCAAGTCCAATGGAACTCCCCCTCAACATGTTTCCTCATGAAAGTACACTTTTGCTTCCCACCAAAGCAAACTCAAGCATAACTGAATCCAACAGCCTCTCTGAAACCTACAATCCCCAAAAATCGCACGCCTCTTCCTCTGTGGACTCCTTATTCGATGCGGTTTCTTCACCTGAGTTTTCGAACATGAACATGGCTGAATCTGGCTGCATTCCTTTTGTGAAGCAGCAGCCTCTGGTGCAAGAATATAATGGCATTGTATCTGCTGGTGTAGTCTCTTCACGGTGGGATGATCAAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1635

Protein Analysis

203

Amino Acids

22.75

Weight (kDa)

4.67

Isoelectric Point (pI)

62.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1635 PF07795 39 - 165 4e-19 Protein of unknown function (DUF1635)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 180, 204
AccIII TCCGGA 1 cut(s) 195
AciI CCGC 1 cut(s) 467
AclI AACGTT 1 cut(s) 240
AclWI GGATC 2 cut(s) 101, 279
AcuI CTGAAG 1 cut(s) 149
AfaI GTAC 2 cut(s) 146, 352
AfeI AGCGCT 1 cut(s) 118
AflIII ACRYGT 1 cut(s) 333
AgsI TTSAA 2 cut(s) 65, 309
AhlI ACTAGT 1 cut(s) 223
AluBI AGCT 4 cut(s) 127, 139, 160, 277
AluI AGCT 4 cut(s) 127, 139, 160, 277
Alw26I GTCTC 1 cut(s) 586
AlwI GGATC 2 cut(s) 101, 279
AlwNI CAGNNNCTG 1 cut(s) 541
Aor13HI TCCGGA 1 cut(s) 195
Aor51HI AGCGCT 1 cut(s) 118
ApeKI GCWGC 3 cut(s) 513, 532, 535
ArsI GACNNNNNNTTYG 2 cut(s) 443, 475
Asp700I GAANNNNTTC 1 cut(s) 69
AspLEI GCGC 2 cut(s) 119, 182
AsuHPI GGTGA 2 cut(s) 45, 468
AsuII TTCGAA 1 cut(s) 488
BarI GAAGNNNNNNTAC 2 cut(s) 128, 160
BbsI GAAGAC 1 cut(s) 62
BbvCI CCTCAGC 1 cut(s) 120
BbvI GCAGC 3 cut(s) 500, 544, 547
BceAI ACGGC 1 cut(s) 251
BclI TGATCA 1 cut(s) 598
BcoDI GTCTC 1 cut(s) 586
BcuI ACTAGT 1 cut(s) 223
BfaI CTAG 2 cut(s) 161, 224
BfoI RGCGCY 1 cut(s) 120
BisI GCNGC 3 cut(s) 514, 533, 536
BlsI GCNGC 3 cut(s) 515, 534, 537
BmrI ACTGGG 1 cut(s) 95
BmsI GCATC 2 cut(s) 253, 454
BmuI ACTGGG 1 cut(s) 95
BpiI GAAGAC 1 cut(s) 62
Bpu10I CCTNAGC 1 cut(s) 120
Bpu14I TTCGAA 1 cut(s) 488
BpuEI CTTGAG 2 cut(s) 176, 364
BsaJI CCNNGG 1 cut(s) 267
BsaWI WCCGGW 1 cut(s) 195
Bse1I ACTGG 1 cut(s) 90
BseAI TCCGGA 1 cut(s) 195
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 2 cut(s) 193, 601
BseMII CTCAG 3 cut(s) 102, 111, 471
BseNI ACTGG 1 cut(s) 90
BseRI GAGGAG 2 cut(s) 137, 221
BseXI GCAGC 3 cut(s) 500, 544, 547
Bsh1236I CGCG 2 cut(s) 180, 204
BsiSI CCGG 1 cut(s) 196
BsmAI GTCTC 1 cut(s) 586
BsmI GAATGC 1 cut(s) 516
Bsp119I TTCGAA 1 cut(s) 488
Bsp13I TCCGGA 1 cut(s) 195
Bsp143I GATC 4 cut(s) 106, 271, 297, 598
BspACI CCGC 1 cut(s) 467
BspCNI CTCAG 3 cut(s) 103, 112, 472
BspEI TCCGGA 1 cut(s) 195
BspFNI CGCG 2 cut(s) 180, 204
BspHI TCATGA 1 cut(s) 343
BspPI GGATC 2 cut(s) 101, 279
BspT104I TTCGAA 1 cut(s) 488
BsrI ACTGG 1 cut(s) 90
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 4 cut(s) 106, 271, 297, 598
Bst4CI ACNGT 1 cut(s) 591
Bst6I CTCTTC 3 cut(s) 38, 445, 589
BstBI TTCGAA 1 cut(s) 488
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 3 cut(s) 111, 120, 480
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 2 cut(s) 193, 601
BstFNI CGCG 2 cut(s) 180, 204
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 2 cut(s) 119, 182
BstKTI GATC 4 cut(s) 109, 274, 300, 601
BstMAI GTCTC 1 cut(s) 586
BstMBI GATC 4 cut(s) 106, 271, 297, 598
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstNSI RCATGY 1 cut(s) 337
BstUI CGCG 2 cut(s) 180, 204
BstV1I GCAGC 3 cut(s) 500, 544, 547
BstV2I GAAGAC 1 cut(s) 62
BtgI CCRYGG 1 cut(s) 267
BtsCI GGATG 2 cut(s) 193, 601
Cac8I GCNNGC 1 cut(s) 435
CaiI CAGNNNCTG 1 cut(s) 541
CciI TCATGA 1 cut(s) 343
CfoI GCGC 2 cut(s) 119, 182
Csp6I GTAC 2 cut(s) 145, 351
CviAII CATG 5 cut(s) 301, 334, 344, 493, 499
CviJI RGCY 8 cut(s) 127, 139, 160, 277, 402, 503, 513, 538
CviKI_1 RGCY 8 cut(s) 127, 139, 160, 277, 402, 503, 513, 538
CviQI GTAC 2 cut(s) 145, 351
DdeI CTNAG 3 cut(s) 111, 120, 480
DpnI GATC 4 cut(s) 108, 273, 299, 600
DpnII GATC 4 cut(s) 106, 271, 297, 598
Eam1104I CTCTTC 3 cut(s) 38, 445, 589
EarI CTCTTC 3 cut(s) 38, 445, 589
Eco47III AGCGCT 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 149
FaeI CATG 5 cut(s) 304, 337, 347, 496, 502
FaiI YATR 9 cut(s) 247, 290, 302, 335, 345, 386, 494, 500, 555
FatI CATG 5 cut(s) 300, 333, 343, 492, 498
FbaI TGATCA 1 cut(s) 598
Fnu4HI GCNGC 3 cut(s) 514, 533, 536
FokI GGATG 1 cut(s) 200
Fsp4HI GCNGC 3 cut(s) 514, 533, 536
FspBI CTAG 2 cut(s) 161, 224
GlaI GCGC 2 cut(s) 118, 181
GluI GCNGC 3 cut(s) 514, 533, 536
HaeII RGCGCY 1 cut(s) 120
HapII CCGG 1 cut(s) 196
HhaI GCGC 2 cut(s) 119, 182
Hin1II CATG 5 cut(s) 304, 337, 347, 496, 502
Hin6I GCGC 2 cut(s) 117, 180
HinP1I GCGC 2 cut(s) 117, 180
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 4 cut(s) 164, 392, 452, 506
HpaII CCGG 1 cut(s) 196
HphI GGTGA 2 cut(s) 45, 468
Hpy166II GTNNAC 3 cut(s) 99, 353, 451
Hpy188I TCNGA 1 cut(s) 409
Hpy188III TCNNGA 3 cut(s) 110, 196, 344
Hpy8I GTNNAC 3 cut(s) 99, 353, 451
HpyAV CCTTC 3 cut(s) 38, 48, 315
HpyCH4III ACNGT 1 cut(s) 591
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 3 cut(s) 245, 516, 547
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 3 cut(s) 111, 120, 480
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 5 cut(s) 304, 337, 347, 496, 502
HspAI GCGC 2 cut(s) 117, 180
Kpn2I TCCGGA 1 cut(s) 195
Ksp22I TGATCA 1 cut(s) 598
Kzo9I GATC 4 cut(s) 106, 271, 297, 598
LmnI GCTCC 1 cut(s) 124
LpnPI CCDG 8 cut(s) 69, 71, 123, 209, 492, 495, 527, 558
Lsp1109I GCAGC 3 cut(s) 500, 544, 547
LweI GCATC 2 cut(s) 253, 454
MaeI CTAG 2 cut(s) 161, 224
MaeII ACGT 1 cut(s) 240
MalI GATC 4 cut(s) 108, 273, 299, 600
MboI GATC 4 cut(s) 106, 271, 297, 598
MboII GAAGA 9 cut(s) 13, 25, 33, 62, 224, 229, 432, 465, 576
MlyI GAGTC 1 cut(s) 446
MmeI TCCRAC 1 cut(s) 420
MroI TCCGGA 1 cut(s) 195
MroXI GAANNNNTTC 1 cut(s) 69
MslI CAYNNNNRTG 1 cut(s) 497
MspI CCGG 1 cut(s) 196
Mva1269I GAATGC 1 cut(s) 516
MvnI CGCG 2 cut(s) 180, 204
MwoI GCNNNNNNNGC 1 cut(s) 544
NdeII GATC 4 cut(s) 106, 271, 297, 598
NlaIII CATG 5 cut(s) 304, 337, 347, 496, 502
NspI RCATGY 1 cut(s) 337
NspV TTCGAA 1 cut(s) 488
PagI TCATGA 1 cut(s) 343
PciI ACATGT 1 cut(s) 333
PctI GAATGC 1 cut(s) 516
PdmI GAANNNNTTC 1 cut(s) 69
PfeI GAWTC 3 cut(s) 164, 392, 506
PkrI GCNGC 3 cut(s) 515, 534, 537
PleI GAGTC 1 cut(s) 446
PpsI GAGTC 1 cut(s) 446
PscI ACATGT 1 cut(s) 333
Psp1406I AACGTT 1 cut(s) 240
PstNI CAGNNNCTG 1 cut(s) 541
RsaI GTAC 2 cut(s) 146, 352
RsaNI GTAC 2 cut(s) 145, 351
RseI CAYNNNNRTG 1 cut(s) 497
SatI GCNGC 3 cut(s) 514, 533, 536
Sau3AI GATC 4 cut(s) 106, 271, 297, 598
SchI GAGTC 1 cut(s) 446
SetI ASST 9 cut(s) 63, 129, 141, 162, 243, 279, 416, 481, 608
SfaNI GCATC 2 cut(s) 253, 454
SfuI TTCGAA 1 cut(s) 488
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 2 cut(s) 155, 379
SmoI CTYRAG 2 cut(s) 155, 379
SpeI ACTAGT 1 cut(s) 223
SsiI CCGC 1 cut(s) 467
SspMI CTAG 2 cut(s) 161, 224
TaaI ACNGT 1 cut(s) 591
TaiI ACGT 1 cut(s) 243
TaqI TCGA 2 cut(s) 462, 488
TatI WGTACW 1 cut(s) 350
TfiI GAWTC 3 cut(s) 164, 392, 506
TseI GCWGC 3 cut(s) 513, 532, 535
TspDTI ATGAA 4 cut(s) 204, 277, 360, 509
TspGWI ACGGA 1 cut(s) 200
XceI RCATGY 1 cut(s) 337
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 2 cut(s) 161, 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.